Rw5G028660

F-box protein At2g27310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011837
Physical Location & Seq
Reverse (-)
43429648 .. 43431391
1744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028660.1

Sequence Viewer

Length: 1020 bp
ATGGCTTCTTCTGGTGCAGCGACTACCGTTGAACAAGGCTGCGACGCCTCCATCTCCGCCCTCCACTCCGACATAATCACGTCCCACATCCTCACCCGCCTCGACGGCCCCACCCTCGCCTCCGCCGCTTGTGCTTCCTCACAATTGCACGCCTTCTCCGCCGAACAGACACTCTGGAAGGACATCTGCGCCTCCACGTGGCCTTCCGTCTCCGATGAACGTGTTGAAGGTATCATATCCACGTTCCCGTCCGGCCACCGCTCGTTTTTCTCTGACTCCTTCCCTCTTCTTGATCACTCCTTCTCGTCCTCGTGTAATCCCAGCCGCTTGGCTCCGGCATCGGAGCTGATCTCCGCCGTGGATATTTTCTACAAGGGAGAGCTGGTTTTCTCCAAGGTTCAAGAGACTGAGACGGAGACCGGGTGGTTCCTATGCTCACCCTTCCGGATCGACCTGCTAGAGCAGAAAGAGACTGTTCCAACTCCGATACGACACATCGGAGACACCACCGAGTCCCTTAAGCACTTGGAGGAGAACTTGACATTGAGCTGGATCGTGATCGACCCGGCCCAAAAGCGCGCGGCCAATCTTTCCAGCCGGAAGCCAGTGTCGGTGACCCGGCACTGGCTCACCGGTGAGGTACAGCTCCGTTTCGCTACGATCATGCCCGGCGATCGTCGCGGGGAGTTCGTGCAGTGCGGGATGGTGGTCACGTGCAAGGGAAGCGACGGAGGGGAGCTACACGTGAGAGAGGTGAGCATGCAGGTGGAGGCCATGGAGGGGAACCACCTGAACGGGAAGGAGAGTTTGGTAATTTTGCAAGGAGCGATTGAGGGAGGGAAGAGGAAGAGGGAGATCGAGGAGGGAAGTGAAGGGAAGGAGAGGTTTGGGGAGTTTCTGGAGATGAAGAGAGAAAGGAAAGAGAGGTCGCAGAGGAGAGAGAGGGCTTTGGATATGATCTGCATTGCTACTGGGGTTACTTTGTTTATGGCGTTTTGGTCTTTCGTCTTGTTCAGATAG

Protein Analysis

339

Amino Acids

37.6

Weight (kDa)

5.65

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27310
fragaria_vesca FvH4_3g25740
malus_domestica MD03G1150200.v1.1 MD03G1150400.v1.1 MD11G1169600.v1.1 MD11G1169800.v1.1
prunus_persica Prupe.6G137300_v2.0.a1
pyrus_communis pycom03g10430 pycom11g14220
rosa_chinensis RchiOBHm_Chr5g0046101
rosa_laevigata RLG00000034384
rosa_multiflora Rmu_sc0001035.1_g000053
rosa_roxburghii Rroxscaffold_1G00035360
rosa_rugosa Rorug05G0225800
rosa_samantha Rh5AG306500 Rh5BG314900 Rh5CG341200 Rh5DG325100
rosa_wichuraiana Rw5G028660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 754
Acc36I ACCTGC 2 cut(s) 462, 754
AccBSI CCGCTC 1 cut(s) 261
AccII CGCG 3 cut(s) 579, 581, 681
AccIII TCCGGA 1 cut(s) 444
AclWI GGATC 2 cut(s) 455, 560
AcoI YGGCCR 2 cut(s) 253, 582
AcvI CACGTG 3 cut(s) 198, 714, 745
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 1 cut(s) 642
AfiI CCNNNNNNNGG 3 cut(s) 198, 624, 780
AflII CTTAAG 1 cut(s) 518
AflIII ACRYGT 2 cut(s) 220, 742
AgeI ACCGGT 1 cut(s) 632
AgsI TTSAA 3 cut(s) 32, 227, 401
AjiI CACGTC 1 cut(s) 81
AjuI GAANNNNNNNTTGG 2 cut(s) 791, 823
AluBI AGCT 5 cut(s) 346, 382, 549, 646, 739
AluI AGCT 5 cut(s) 346, 382, 549, 646, 739
Alw26I GTCTC 6 cut(s) 214, 398, 404, 410, 464, 495
AlwI GGATC 2 cut(s) 455, 560
Aor13HI TCCGGA 1 cut(s) 444
AoxI GGCC 6 cut(s) 106, 200, 253, 567, 582, 771
ApeKI GCWGC 2 cut(s) 17, 39
AsiGI ACCGGT 1 cut(s) 632
AspLEI GCGC 3 cut(s) 191, 579, 581
AspS9I GGNCC 2 cut(s) 107, 568
AsuC2I CCSGG 4 cut(s) 421, 566, 619, 669
AsuHPI GGTGA 6 cut(s) 85, 429, 622, 625, 647, 766
BauI CACGAG 1 cut(s) 310
BbrPI CACGTG 3 cut(s) 198, 714, 745
BbvI GCAGC 2 cut(s) 26, 29
BccI CCATC 2 cut(s) 59, 697
BceAI ACGGC 2 cut(s) 121, 341
BclI TGATCA 1 cut(s) 292
BcnI CCSGG 4 cut(s) 421, 566, 619, 669
BcoDI GTCTC 6 cut(s) 214, 398, 404, 410, 464, 495
BfaI CTAG 1 cut(s) 458
BfrI CTTAAG 1 cut(s) 518
BfuAI ACCTGC 2 cut(s) 462, 754
BglI GCCNNNNNGGC 1 cut(s) 105
BisI GCNGC 5 cut(s) 18, 40, 126, 325, 582
BlsI GCNGC 5 cut(s) 19, 41, 127, 326, 583
Bme1390I CCNGG 4 cut(s) 421, 566, 619, 669
BmgBI CACGTC 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 107, 568
BmiI GGNNCC 4 cut(s) 109, 333, 428, 785
BmrFI CCNGG 4 cut(s) 421, 566, 619, 669
BmrI ACTGGG 1 cut(s) 981
BmsI GCATC 1 cut(s) 347
BmuI ACTGGG 1 cut(s) 981
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 1 cut(s) 920
BpuMI CCSGG 4 cut(s) 421, 566, 619, 669
BsaAI YACGTR 3 cut(s) 198, 714, 745
BsaBI GATNNNNATC 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 357, 393, 774
BsaWI WCCGGW 2 cut(s) 444, 632
BsaXI ACNNNNNCTCC 6 cut(s) 140, 170, 369, 399, 407, 437
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 624, 780
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 3 cut(s) 605, 629, 976
Bse3DI GCAATG 1 cut(s) 963
Bse8I GATNNNNATC 1 cut(s) 557
BseAI TCCGGA 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 357, 393, 774
BseGI GGATG 2 cut(s) 87, 708
BseJI GATNNNNATC 1 cut(s) 557
BseLI CCNNNNNNNGG 3 cut(s) 198, 624, 780
BseMI GCAATG 1 cut(s) 963
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 3 cut(s) 605, 629, 976
BsePI GCGCGC 1 cut(s) 577
BseRI GAGGAG 3 cut(s) 545, 875, 949
BseXI GCAGC 2 cut(s) 26, 29
BseYI CCCAGC 1 cut(s) 320
BsgI GTGCAG 2 cut(s) 36, 713
Bsh1236I CGCG 3 cut(s) 579, 581, 681
Bsh1285I CGRYCG 1 cut(s) 676
BshFI GGCC 6 cut(s) 108, 202, 255, 569, 584, 773
BshTI ACCGGT 1 cut(s) 632
BsiEI CGRYCG 1 cut(s) 676
BsiSI CCGG 9 cut(s) 252, 335, 420, 445, 566, 598, 619, 633, 669
BslFI GGGAC 2 cut(s) 67, 499
BslI CCNNNNNNNGG 3 cut(s) 198, 624, 780
BsmAI GTCTC 6 cut(s) 214, 398, 404, 410, 464, 495
BsmBI CGTCTC 2 cut(s) 214, 404
BsmFI GGGAC 2 cut(s) 67, 499
BsnI GGCC 6 cut(s) 108, 202, 255, 569, 584, 773
Bso31I GGTCTC 1 cut(s) 410
Bsp13I TCCGGA 1 cut(s) 444
Bsp143I GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
Bsp19I CCATGG 1 cut(s) 774
BspANI GGCC 6 cut(s) 108, 202, 255, 569, 584, 773
BspCNI CTCAG 1 cut(s) 400
BspEI TCCGGA 1 cut(s) 444
BspFNI CGCG 3 cut(s) 579, 581, 681
BspLI GGNNCC 4 cut(s) 109, 333, 428, 785
BspMI ACCTGC 2 cut(s) 462, 754
BspPI GGATC 2 cut(s) 455, 560
BspTI CTTAAG 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 410
BsrBI CCGCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 963
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 3 cut(s) 605, 629, 976
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 3 cut(s) 357, 393, 774
BssHII GCGCGC 1 cut(s) 577
BssMI GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
BssNI GRCGYC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 310
BssT1I CCWWGG 2 cut(s) 393, 774
Bst2BI CACGAG 1 cut(s) 310
Bst4CI ACNGT 2 cut(s) 28, 475
Bst6I CTCTTC 4 cut(s) 291, 836, 842, 902
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 518
BstBAI YACGTR 3 cut(s) 198, 714, 745
BstC8I GCNNGC 3 cut(s) 150, 579, 761
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 2 cut(s) 357, 774
BstEII GGTNACC 1 cut(s) 613
BstF5I GGATG 2 cut(s) 87, 708
BstFNI CGCG 3 cut(s) 579, 581, 681
BstHHI GCGC 3 cut(s) 191, 579, 581
BstKTI GATC 9 cut(s) 295, 351, 450, 555, 561, 663, 676, 858, 960
BstMAI GTCTC 6 cut(s) 214, 398, 404, 410, 464, 495
BstMBI GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
BstMCI CGRYCG 1 cut(s) 676
BstMWI GCNNNNNNNGC 6 cut(s) 105, 125, 131, 158, 678, 723
BstNSI RCATGY 1 cut(s) 763
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 4 cut(s) 419, 564, 617, 667
BstUI CGCG 3 cut(s) 579, 581, 681
BstV1I GCAGC 2 cut(s) 26, 29
BstXI CCANNNNNNTGG 1 cut(s) 328
BsuRI GGCC 6 cut(s) 108, 202, 255, 569, 584, 773
BtgI CCRYGG 2 cut(s) 357, 774
BtrI CACGTC 1 cut(s) 81
BtsCI GGATG 2 cut(s) 87, 708
BtsI GCAGTG 1 cut(s) 701
BtsIMutI CAGTG 3 cut(s) 612, 622, 701
BveI ACCTGC 2 cut(s) 462, 754
Cac8I GCNNGC 3 cut(s) 150, 579, 761
CfoI GCGC 3 cut(s) 191, 579, 581
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 2 cut(s) 107, 568
CseI GACGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 641
CspAI ACCGGT 1 cut(s) 632
CviAII CATG 3 cut(s) 664, 760, 775
CviQI GTAC 1 cut(s) 641
DdeI CTNAG 1 cut(s) 408
DpnI GATC 9 cut(s) 294, 350, 449, 554, 560, 662, 675, 857, 959
DpnII GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
EaeI YGGCCR 2 cut(s) 253, 582
Eam1104I CTCTTC 4 cut(s) 291, 836, 842, 902
EarI CTCTTC 4 cut(s) 291, 836, 842, 902
EciI GGCGGA 4 cut(s) 46, 112, 148, 343
Eco130I CCWWGG 2 cut(s) 393, 774
Eco31I GGTCTC 1 cut(s) 410
Eco72I CACGTG 3 cut(s) 198, 714, 745
Eco91I GGTNACC 1 cut(s) 613
EcoO65I GGTNACC 1 cut(s) 613
EcoT14I CCWWGG 2 cut(s) 393, 774
ErhI CCWWGG 2 cut(s) 393, 774
Esp3I CGTCTC 2 cut(s) 214, 404
FaeI CATG 3 cut(s) 667, 763, 778
FaiI YATR 8 cut(s) 74, 236, 433, 665, 761, 776, 956, 989
FaqI GGGAC 2 cut(s) 67, 499
FatI CATG 3 cut(s) 663, 759, 774
FauI CCCGC 3 cut(s) 104, 674, 692
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 5 cut(s) 18, 40, 126, 325, 582
FokI GGATG 2 cut(s) 74, 715
Fsp4HI GCNGC 5 cut(s) 18, 40, 126, 325, 582
FspBI CTAG 1 cut(s) 458
GlaI GCGC 3 cut(s) 190, 578, 580
GluI GCNGC 5 cut(s) 18, 40, 126, 325, 582
GsaI CCCAGC 1 cut(s) 324
GsuI CTGGAG 1 cut(s) 920
HaeIII GGCC 6 cut(s) 108, 202, 255, 569, 584, 773
HapII CCGG 9 cut(s) 252, 335, 420, 445, 566, 598, 619, 633, 669
HgaI GACGC 1 cut(s) 53
HhaI GCGC 3 cut(s) 191, 579, 581
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 667, 763, 778
Hin6I GCGC 3 cut(s) 189, 577, 579
HinP1I GCGC 3 cut(s) 189, 577, 579
HinfI GANTC 2 cut(s) 275, 512
HpaII CCGG 9 cut(s) 252, 335, 420, 445, 566, 598, 619, 633, 669
HphI GGTGA 6 cut(s) 85, 429, 622, 625, 647, 766
Hpy188I TCNGA 7 cut(s) 70, 214, 274, 343, 486, 500, 1016
Hpy188III TCNNGA 6 cut(s) 175, 290, 401, 445, 556, 899
Hpy99I CGWCG 4 cut(s) 47, 107, 681, 731
HpyCH4III ACNGT 2 cut(s) 28, 475
HpyCH4IV ACGT 6 cut(s) 80, 197, 220, 242, 713, 744
HpyCH4V TGCA 7 cut(s) 17, 148, 694, 717, 763, 820, 963
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 125, 131, 158, 678, 723
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 6 cut(s) 80, 197, 220, 242, 713, 744
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 667, 763, 778
HspAI GCGC 3 cut(s) 189, 577, 579
Kpn2I TCCGGA 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
LmnI GCTCC 5 cut(s) 337, 343, 651, 736, 824
Lsp1109I GCAGC 2 cut(s) 26, 29
LweI GCATC 1 cut(s) 347
MaeI CTAG 1 cut(s) 458
MaeII ACGT 6 cut(s) 80, 197, 220, 242, 713, 744
MaeIII GTNAC 3 cut(s) 613, 709, 976
MalI GATC 9 cut(s) 294, 350, 449, 554, 560, 662, 675, 857, 959
MbiI CCGCTC 1 cut(s) 261
MboI GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
MboII GAAGA 4 cut(s) 278, 853, 859, 919
MfeI CAATTG 1 cut(s) 143
MluCI AATT 2 cut(s) 143, 813
MlyI GAGTC 2 cut(s) 269, 521
MmeI TCCRAC 2 cut(s) 93, 503
MroI TCCGGA 1 cut(s) 444
MseI TTAA 1 cut(s) 519
MslI CAYNNNNRTG 1 cut(s) 764
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 9 cut(s) 252, 335, 420, 445, 566, 598, 619, 633, 669
MspR9I CCNGG 4 cut(s) 421, 566, 619, 669
MunI CAATTG 1 cut(s) 143
MvnI CGCG 3 cut(s) 579, 581, 681
MwoI GCNNNNNNNGC 6 cut(s) 105, 125, 131, 158, 678, 723
NciI CCSGG 4 cut(s) 421, 566, 619, 669
NcoI CCATGG 1 cut(s) 774
NdeII GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
NlaIII CATG 3 cut(s) 667, 763, 778
NlaIV GGNNCC 4 cut(s) 109, 333, 428, 785
NmuCI GTSAC 2 cut(s) 613, 709
NspI RCATGY 1 cut(s) 763
PaeI GCATGC 1 cut(s) 763
PaqCI CACCTGC 1 cut(s) 754
PauI GCGCGC 1 cut(s) 577
PinAI ACCGGT 1 cut(s) 632
PkrI GCNGC 5 cut(s) 19, 41, 127, 326, 583
Ple19I CGATCG 1 cut(s) 676
PleI GAGTC 2 cut(s) 269, 520
PmaCI CACGTG 3 cut(s) 198, 714, 745
PmlI CACGTG 3 cut(s) 198, 714, 745
PpsI GAGTC 2 cut(s) 269, 520
Ppu21I YACGTR 3 cut(s) 198, 714, 745
PspCI CACGTG 3 cut(s) 198, 714, 745
PspEI GGTNACC 1 cut(s) 613
PspFI CCCAGC 1 cut(s) 320
PspN4I GGNNCC 4 cut(s) 109, 333, 428, 785
PspPI GGNCC 2 cut(s) 107, 568
PteI GCGCGC 1 cut(s) 577
PvuI CGATCG 1 cut(s) 676
RsaI GTAC 1 cut(s) 642
RsaNI GTAC 1 cut(s) 641
RseI CAYNNNNRTG 1 cut(s) 764
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 5 cut(s) 18, 40, 126, 325, 582
Sau3AI GATC 9 cut(s) 292, 348, 447, 552, 558, 660, 673, 855, 957
Sau96I GGNCC 2 cut(s) 107, 568
SchI GAGTC 2 cut(s) 269, 521
ScrFI CCNGG 4 cut(s) 421, 566, 619, 669
SfaNI GCATC 1 cut(s) 347
SgrAI CRCCGGYG 1 cut(s) 632
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SphI GCATGC 1 cut(s) 763
Sse9I AATT 2 cut(s) 143, 813
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 4 cut(s) 419, 564, 617, 667
StyI CCWWGG 2 cut(s) 393, 774
TaaI ACNGT 2 cut(s) 28, 475
TaiI ACGT 6 cut(s) 83, 200, 223, 245, 716, 747
TaqI TCGA 4 cut(s) 102, 450, 561, 858
TasI AATT 2 cut(s) 143, 813
TauI GCSGC 3 cut(s) 128, 327, 584
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 3 cut(s) 612, 629, 701
TseFI GTSAC 2 cut(s) 613, 709
TseI GCWGC 2 cut(s) 17, 39
Tsp45I GTSAC 2 cut(s) 613, 709
TspDTI ATGAA 2 cut(s) 231, 920
TspGWI ACGGA 4 cut(s) 196, 428, 638, 744
TspRI CASTG 3 cut(s) 612, 629, 701
Vha464I CTTAAG 1 cut(s) 518
XceI RCATGY 1 cut(s) 763
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.