MD11G1169600.v1.1

F-box protein At2g27310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011837
Physical Location & Seq
Reverse (-)
18182608 .. 18183618
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1169600.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGAGCTTCTCTGCTGCTTCAACCACACCGGCCGCCGACAAGGGCAACGGAGCTTCAATCTCCGCCGTCCATCACGACATCATCACGGCCCACATCCTCACCCGCCTCAACGGCCAGACCTTGGCCGCTGTGGCCTGCGCATCGTCCGAACTACGCGCTTTCTCCACCGAAGGAAAACTCTGGCGGGACATCAGCACCGCCATGTGGCCTTCAACCACCGATACACGTGTTAACGACCTCATTTCCACTTTCCCAGCCGGTCACCGCTCTTTTTTCTCCGACTCTTTCCCGCTCCTCGACTACTCCCCCTCCCAATTCGCTCTCAGCCCTTCGTCTCGCACCGCGGAGTTAATCTCGGCCGTCGATATTTTCTACAAAGGCCAGCTCATTTTCTCTAAGGTTCAGGAATCAGAGACCGAATCCGGGTGGTTCTTATGCTCGCCCTTCCGGGTGGACTTGCTTGACCCGAAGGAAACCTTTCCGACACCAATCCGACATGTCGGAGAAGGCCAAAAGTCATTGAAGCACTTGGAGGAGAACTTGAGCTTGAGCTGGATCGTGATCGATCCAACCCGGAAACGGGCGGCGAATTTGTCGAGCCGGAGGCCGGTGACGGTGCAGCAGCACTGGCTGACGGGGGAAATACAGCTGCGGTTCACGACGATATTGGCGGGGGAGAAGAAGGGTGAGTATGTGCAGTGCGGTACGGTGGTCACGTGCAAGGGGACCGAAGGTGGGGAGCTGCACGTGAGGGAGGTGAGCATGCATGTGGAGGCCATGGAGGAGAATCATTTGAACGGGAGGGAGAGCTTGGTAATATTGCAGAGGGCGATCGAAGGAGGGAAGAGGAGGAAGGAGATAAACGGGAAGGCGAGGTTGGAGGAGTACTTGGAGATGAAGAGAGAGAGGAGGAAGAGAAACGAGAGGAGAGAGAAGGCTTTCGACATGATCTGCCTTGCTGCTGGGGTTACTATTTTCATGGCGTTCTGGTCATTCGTCTTGTTCAGATAG

Protein Analysis

337

Amino Acids

37.71

Weight (kDa)

8.55

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27310
fragaria_vesca FvH4_3g25740
malus_domestica MD03G1150200.v1.1 MD03G1150400.v1.1 MD11G1169600.v1.1 MD11G1169800.v1.1
prunus_persica Prupe.6G137300_v2.0.a1
pyrus_communis pycom03g10430 pycom11g14220
rosa_chinensis RchiOBHm_Chr5g0046101
rosa_laevigata RLG00000034384
rosa_multiflora Rmu_sc0001035.1_g000053
rosa_roxburghii Rroxscaffold_1G00035360
rosa_rugosa Rorug05G0225800
rosa_samantha Rh5AG306500 Rh5BG314900 Rh5CG341200 Rh5DG325100
rosa_wichuraiana Rw5G028660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 121
AccBSI CCGCTC 2 cut(s) 267, 292
AccII CGCG 2 cut(s) 156, 344
AclWI GGATC 2 cut(s) 560, 563
AcoI YGGCCR 4 cut(s) 30, 112, 123, 357
AcsI RAATTY 1 cut(s) 589
AcvI CACGTG 3 cut(s) 227, 717, 748
AfaI GTAC 2 cut(s) 706, 887
AfiI CCNNNNNNNGG 7 cut(s) 121, 204, 423, 579, 580, 607, 735
AflIII ACRYGT 3 cut(s) 224, 226, 496
AgsI TTSAA 5 cut(s) 21, 57, 213, 523, 796
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 8 cut(s) 6, 53, 385, 546, 552, 649, 742, 810
AluI AGCT 8 cut(s) 6, 53, 385, 546, 552, 649, 742, 810
Alw26I GTCTC 2 cut(s) 339, 407
AlwI GGATC 2 cut(s) 560, 563
ApeKI GCWGC 6 cut(s) 14, 619, 622, 649, 742, 959
ApoI RAATTY 1 cut(s) 589
Asp700I GAANNNNTTC 2 cut(s) 477, 938
AspLEI GCGC 2 cut(s) 140, 158
AspS9I GGNCC 2 cut(s) 88, 726
AsuC2I CCSGG 3 cut(s) 424, 449, 574
AsuHPI GGTGA 5 cut(s) 91, 254, 622, 698, 769
AvaII GGWCC 1 cut(s) 726
BaeI ACNNNNGTAYC 4 cut(s) 213, 246, 696, 729
BbrPI CACGTG 3 cut(s) 227, 717, 748
BbvI GCAGC 5 cut(s) 631, 634, 636, 729, 946
BccI CCATC 1 cut(s) 78
BceAI ACGGC 4 cut(s) 50, 102, 127, 344
BcnI CCSGG 3 cut(s) 424, 449, 574
BcoDI GTCTC 2 cut(s) 339, 407
BglI GCCNNNNNGGC 2 cut(s) 111, 131
BisI GCNGC 9 cut(s) 15, 33, 126, 585, 620, 623, 650, 743, 960
BlsI GCNGC 9 cut(s) 16, 34, 127, 586, 621, 624, 651, 744, 961
BmcAI AGTACT 1 cut(s) 887
Bme1390I CCNGG 3 cut(s) 424, 449, 574
Bme18I GGWCC 1 cut(s) 726
BmgT120I GGNCC 2 cut(s) 88, 726
BmiI GGNNCC 1 cut(s) 727
BmrFI CCNGG 3 cut(s) 424, 449, 574
BmsI GCATC 1 cut(s) 149
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpuEI CTTGAG 2 cut(s) 562, 568
BpuMI CCSGG 3 cut(s) 424, 449, 574
Bsa29I ATCGAT 1 cut(s) 564
BsaAI YACGTR 3 cut(s) 227, 717, 748
BsaBI GATNNNNATC 1 cut(s) 560
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 3 cut(s) 120, 342, 777
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 7 cut(s) 121, 204, 423, 579, 580, 607, 735
Bse118I RCCGGY 3 cut(s) 28, 257, 607
Bse1I ACTGG 1 cut(s) 632
Bse8I GATNNNNATC 1 cut(s) 560
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 120, 342, 777
BseGI GGATG 1 cut(s) 93
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 7 cut(s) 121, 204, 423, 579, 580, 607, 735
BseMII CTCAG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 632
BseRI GAGGAG 7 cut(s) 284, 548, 797, 862, 896, 922, 940
BseX3I CGGCCG 2 cut(s) 30, 357
BseXI GCAGC 5 cut(s) 631, 634, 636, 729, 946
BseYI CCCAGC 2 cut(s) 253, 962
BsgI GTGCAG 3 cut(s) 638, 716, 728
Bsh1236I CGCG 2 cut(s) 156, 344
Bsh1285I CGRYCG 3 cut(s) 33, 360, 834
BshVI ATCGAT 1 cut(s) 564
BsiEI CGRYCG 3 cut(s) 33, 360, 834
BsiSI CCGG 7 cut(s) 29, 258, 423, 448, 574, 601, 608
BslFI GGGAC 2 cut(s) 200, 739
BslI CCNNNNNNNGG 7 cut(s) 121, 204, 423, 579, 580, 607, 735
BsmAI GTCTC 2 cut(s) 339, 407
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 2 cut(s) 200, 739
Bso31I GGTCTC 1 cut(s) 407
Bsp143I GATC 5 cut(s) 555, 561, 565, 831, 948
Bsp19I CCATGG 1 cut(s) 777
BspCNI CTCAG 1 cut(s) 336
BspDI ATCGAT 1 cut(s) 564
BspFNI CGCG 2 cut(s) 156, 344
BspLI GGNNCC 1 cut(s) 727
BspPI GGATC 2 cut(s) 560, 563
BspTNI GGTCTC 1 cut(s) 407
BsrBI CCGCTC 2 cut(s) 267, 292
BsrFI RCCGGY 3 cut(s) 28, 257, 607
BsrI ACTGG 1 cut(s) 632
BssAI RCCGGY 3 cut(s) 28, 257, 607
BssECI CCNNGG 3 cut(s) 120, 342, 777
BssMI GATC 5 cut(s) 555, 561, 565, 831, 948
BssT1I CCWWGG 2 cut(s) 120, 777
Bst4CI ACNGT 2 cut(s) 616, 709
Bst6I CTCTTC 3 cut(s) 839, 893, 908
BstBAI YACGTR 3 cut(s) 227, 717, 748
BstC8I GCNNGC 4 cut(s) 136, 383, 440, 764
BstDEI CTNAG 2 cut(s) 323, 396
BstDSI CCRYGG 2 cut(s) 342, 777
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 156, 344
BstHHI GCGC 2 cut(s) 140, 158
BstKTI GATC 5 cut(s) 558, 564, 568, 834, 951
BstMAI GTCTC 2 cut(s) 339, 407
BstMBI GATC 5 cut(s) 555, 561, 565, 831, 948
BstMCI CGRYCG 3 cut(s) 33, 360, 834
BstMWI GCNNNNNNNGC 3 cut(s) 111, 131, 628
BstNSI RCATGY 3 cut(s) 500, 766, 770
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 422, 447, 572
BstUI CGCG 2 cut(s) 156, 344
BstV1I GCAGC 5 cut(s) 631, 634, 636, 729, 946
BstZI CGGCCG 2 cut(s) 30, 357
Bsu15I ATCGAT 1 cut(s) 564
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 2 cut(s) 342, 777
BtsCI GGATG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 704
BtsIMutI CAGTG 2 cut(s) 625, 704
Cac8I GCNNGC 4 cut(s) 136, 383, 440, 764
CfoI GCGC 2 cut(s) 140, 158
Cfr10I RCCGGY 3 cut(s) 28, 257, 607
Cfr13I GGNCC 2 cut(s) 88, 726
Cfr42I CCGCGG 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 2 cut(s) 705, 886
CviAII CATG 7 cut(s) 202, 497, 763, 767, 778, 946, 979
CviQI GTAC 2 cut(s) 705, 886
DdeI CTNAG 2 cut(s) 323, 396
DpnI GATC 5 cut(s) 557, 563, 567, 833, 950
DpnII GATC 5 cut(s) 555, 561, 565, 831, 948
EaeI YGGCCR 4 cut(s) 30, 112, 123, 357
EagI CGGCCG 2 cut(s) 30, 357
Eam1104I CTCTTC 3 cut(s) 839, 893, 908
EarI CTCTTC 3 cut(s) 839, 893, 908
EciI GGCGGA 1 cut(s) 52
EclXI CGGCCG 2 cut(s) 30, 357
Eco130I CCWWGG 2 cut(s) 120, 777
Eco31I GGTCTC 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 726
Eco52I CGGCCG 2 cut(s) 30, 357
Eco72I CACGTG 3 cut(s) 227, 717, 748
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoT14I CCWWGG 2 cut(s) 120, 777
EcoT22I ATGCAT 1 cut(s) 768
ErhI CCWWGG 2 cut(s) 120, 777
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 7 cut(s) 205, 500, 766, 770, 781, 949, 982
FaiI YATR 9 cut(s) 203, 436, 498, 693, 764, 768, 779, 947, 980
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 2 cut(s) 200, 739
FatI CATG 7 cut(s) 201, 496, 762, 766, 777, 945, 978
FauI CCCGC 4 cut(s) 110, 177, 297, 664
Fnu4HI GCNGC 9 cut(s) 15, 33, 126, 585, 620, 623, 650, 743, 960
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 9 cut(s) 15, 33, 126, 585, 620, 623, 650, 743, 960
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 2 cut(s) 139, 157
GluI GCNGC 9 cut(s) 15, 33, 126, 585, 620, 623, 650, 743, 960
GsaI CCCAGC 2 cut(s) 257, 966
HapII CCGG 7 cut(s) 29, 258, 423, 448, 574, 601, 608
HhaI GCGC 2 cut(s) 140, 158
Hin1II CATG 7 cut(s) 205, 500, 766, 770, 781, 949, 982
Hin6I GCGC 2 cut(s) 138, 156
HinP1I GCGC 2 cut(s) 138, 156
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 4 cut(s) 281, 407, 419, 787
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 7 cut(s) 29, 258, 423, 448, 574, 601, 608
HphI GGTGA 5 cut(s) 91, 254, 622, 698, 769
Hpy166II GTNNAC 3 cut(s) 232, 454, 657
Hpy188I TCNGA 7 cut(s) 148, 280, 412, 483, 494, 503, 1007
Hpy188III TCNNGA 4 cut(s) 74, 404, 559, 658
Hpy8I GTNNAC 3 cut(s) 232, 454, 657
Hpy99I CGWCG 2 cut(s) 365, 664
HpyCH4III ACNGT 2 cut(s) 616, 709
HpyCH4IV ACGT 3 cut(s) 226, 716, 747
HpyCH4V TGCA 6 cut(s) 619, 697, 720, 745, 766, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 131, 628
HpyF3I CTNAG 2 cut(s) 323, 396
HpySE526I ACGT 3 cut(s) 226, 716, 747
Hsp92II CATG 7 cut(s) 205, 500, 766, 770, 781, 949, 982
HspAI GCGC 2 cut(s) 138, 156
KspAI GTTAAC 1 cut(s) 232
KspI CCGCGG 1 cut(s) 345
Kzo9I GATC 5 cut(s) 555, 561, 565, 831, 948
LmnI GCTCC 3 cut(s) 50, 297, 739
Lsp1109I GCAGC 5 cut(s) 631, 634, 636, 729, 946
LweI GCATC 1 cut(s) 149
MaeII ACGT 3 cut(s) 226, 716, 747
MaeIII GTNAC 4 cut(s) 260, 610, 712, 967
MalI GATC 5 cut(s) 557, 563, 567, 833, 950
MbiI CCGCTC 2 cut(s) 267, 292
MboI GATC 5 cut(s) 555, 561, 565, 831, 948
MboII GAAGA 4 cut(s) 691, 856, 910, 925
MluCI AATT 2 cut(s) 314, 589
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 6 cut(s) 303, 481, 506, 517, 593, 858
Mph1103I ATGCAT 1 cut(s) 768
MroXI GAANNNNTTC 2 cut(s) 477, 938
MseI TTAA 2 cut(s) 231, 350
MslI CAYNNNNRTG 2 cut(s) 200, 767
MspA1I CMGCKG 3 cut(s) 128, 344, 649
MspI CCGG 7 cut(s) 29, 258, 423, 448, 574, 601, 608
MspR9I CCNGG 3 cut(s) 424, 449, 574
MvnI CGCG 2 cut(s) 156, 344
MwoI GCNNNNNNNGC 3 cut(s) 111, 131, 628
NciI CCSGG 3 cut(s) 424, 449, 574
NcoI CCATGG 1 cut(s) 777
NdeII GATC 5 cut(s) 555, 561, 565, 831, 948
NlaIII CATG 7 cut(s) 205, 500, 766, 770, 781, 949, 982
NlaIV GGNNCC 1 cut(s) 727
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 3 cut(s) 260, 610, 712
NsbI TGCGCA 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 3 cut(s) 500, 766, 770
PaeI GCATGC 1 cut(s) 766
PciI ACATGT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 713
PdmI GAANNNNTTC 2 cut(s) 477, 938
PfeI GAWTC 3 cut(s) 407, 419, 787
PflMI CCANNNNNTGG 1 cut(s) 121
PkrI GCNGC 9 cut(s) 16, 34, 127, 586, 621, 624, 651, 744, 961
Ple19I CGATCG 1 cut(s) 834
PleI GAGTC 1 cut(s) 275
PmaCI CACGTG 3 cut(s) 227, 717, 748
PmlI CACGTG 3 cut(s) 227, 717, 748
PpsI GAGTC 1 cut(s) 275
Ppu21I YACGTR 3 cut(s) 227, 717, 748
PscI ACATGT 1 cut(s) 496
PspCI CACGTG 3 cut(s) 227, 717, 748
PspEI GGTNACC 1 cut(s) 260
PspFI CCCAGC 2 cut(s) 253, 962
PspN4I GGNNCC 1 cut(s) 727
PspPI GGNCC 2 cut(s) 88, 726
PvuI CGATCG 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 649
RsaI GTAC 2 cut(s) 706, 887
RsaNI GTAC 2 cut(s) 705, 886
RseI CAYNNNNRTG 2 cut(s) 200, 767
SacII CCGCGG 1 cut(s) 345
SaqAI TTAA 2 cut(s) 231, 350
SatI GCNGC 9 cut(s) 15, 33, 126, 585, 620, 623, 650, 743, 960
Sau3AI GATC 5 cut(s) 555, 561, 565, 831, 948
Sau96I GGNCC 2 cut(s) 88, 726
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 424, 449, 574
SfaNI GCATC 1 cut(s) 149
SfiI GGCCNNNNNGGCC 1 cut(s) 131
Sfr303I CCGCGG 1 cut(s) 345
SgrBI CCGCGG 1 cut(s) 345
SinI GGWCC 1 cut(s) 726
SmiMI CAYNNNNRTG 2 cut(s) 200, 767
SmlI CTYRAG 2 cut(s) 541, 547
SmoI CTYRAG 2 cut(s) 541, 547
SphI GCATGC 1 cut(s) 766
Sse9I AATT 2 cut(s) 314, 589
SspI AATATT 1 cut(s) 819
StyD4I CCNGG 3 cut(s) 422, 447, 572
StyI CCWWGG 2 cut(s) 120, 777
TaaI ACNGT 2 cut(s) 616, 709
TaiI ACGT 3 cut(s) 229, 719, 750
TaqI TCGA 6 cut(s) 297, 363, 564, 596, 834, 942
TaqII GACCGA 2 cut(s) 431, 743
TasI AATT 2 cut(s) 314, 589
TatI WGTACW 1 cut(s) 885
TauI GCSGC 3 cut(s) 35, 128, 587
TfiI GAWTC 3 cut(s) 407, 419, 787
Tru1I TTAA 2 cut(s) 231, 350
Tru9I TTAA 2 cut(s) 231, 350
TscAI CASTG 2 cut(s) 632, 704
TseFI GTSAC 3 cut(s) 260, 610, 712
TseI GCWGC 6 cut(s) 14, 619, 622, 649, 742, 959
Tsp45I GTSAC 3 cut(s) 260, 610, 712
TspDTI ATGAA 2 cut(s) 911, 967
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 2 cut(s) 632, 704
Van91I CCANNNNNTGG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 726
XapI RAATTY 1 cut(s) 589
XceI RCATGY 3 cut(s) 500, 766, 770
XmnI GAANNNNTTC 2 cut(s) 477, 938
ZrmI AGTACT 1 cut(s) 887
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.