pycom11g14220

F-box protein At2g27310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011837
Physical Location & Seq
Reverse (-)
14046690 .. 14047700
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g14220.1

Sequence Viewer

Length: 1011 bp
ATGTGCTTCTCTGCTGCTTCAACCACACCGGCCGTCGACAAGGGCGACAGAGCTTCAATCTATGCCGTCCATCCCGACATCATCACGGCCCACATCCTCACCTGCCTCGACGGCCAGACCTTGGCCGCTGTGGCCTGCGCATCGTCCGAACTACGCGCTTTCTCCGCCGACGAAAAACTCTGGCGGGACATCAGCACCGCCATGTGGCCTTCAACCACCGATCCACGTGTTAACGACTTCATTTCCACTTTCCCAGCCGGTCACCGCTCTTTCTTCTCCGACTCTTTCCCGCTCCTCGACCACTCCCCCTCCCAATTCGCTCTCAGCCCTTCGTCTCCCACCGCGGAGTTAATCTCGGCCGTCGATATTTTCTACAAAGGCCAGCTCATTTTCTCTAAGGTTCAGGCATCAGAGACCGAATCCGAGTGGTTCCTCTGCTCGCCCTTCCGGGTGGACTTGCTTGGCCCGAAAGAAACCTTTCCCACACCAATCCGACATGCCGGAGAAGGCCAAAAGTCATTGAAGCACTTGGAGGAGAACTTGAGTTTGAGCTGGATCGTGATCGATCCGACCCGGAAACGGGCGGCGAATTTGTCGAGCCGAAGGCCGGTGACGGTGCAGAAGCACAGGCTGACGGGGGAAATACACCTGCGGTTCGCGACGATATTGGTGGGGGAGAAGAAGGGTGAGTATGTGCAGTGCGGTGCAGTGGTCACGTGCAAGGGGACCGAAGGTGGGGAGCTGCACGTGAGGGAGGTGAGCATGCATGTGGAGGCCATGGAGGGGAATCATTTGAACGGGAGGGAGAGCTTGGTAATATTGCAGACGTCGATCGAAGGAGGGAAGAGGAGCAAGGAGATAAAGGGGAAGGCGAGGTTGGAGGAGTACTTGGAGATGAAGAGAGAGAGGAAAAAGAGAAGGGAGAGGAGAGAGAAGGCTTTGGACATGATCTGCATTGCCACTGGGGTTACTATTTTTATGGCGTTCTGGTCATTCATCTTGTTCAGATAG

Protein Analysis

337

Amino Acids

37.54

Weight (kDa)

8.42

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27310
fragaria_vesca FvH4_3g25740
malus_domestica MD03G1150200.v1.1 MD03G1150400.v1.1 MD11G1169600.v1.1 MD11G1169800.v1.1
prunus_persica Prupe.6G137300_v2.0.a1
pyrus_communis pycom03g10430 pycom11g14220
rosa_chinensis RchiOBHm_Chr5g0046101
rosa_laevigata RLG00000034384
rosa_multiflora Rmu_sc0001035.1_g000053
rosa_roxburghii Rroxscaffold_1G00035360
rosa_rugosa Rorug05G0225800
rosa_samantha Rh5AG306500 Rh5BG314900 Rh5CG341200 Rh5DG325100
rosa_wichuraiana Rw5G028660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 110, 657
AatII GACGTC 1 cut(s) 830
Acc16I TGCGCA 1 cut(s) 139
Acc36I ACCTGC 2 cut(s) 110, 657
AccB7I CCANNNNNTGG 1 cut(s) 121
AccBSI CCGCTC 2 cut(s) 267, 292
AccI GTMKAC 1 cut(s) 36
AccII CGCG 3 cut(s) 156, 344, 659
AclWI GGATC 3 cut(s) 215, 560, 563
AcoI YGGCCR 4 cut(s) 30, 112, 123, 357
AcsI RAATTY 1 cut(s) 589
AcvI CACGTG 3 cut(s) 227, 717, 748
AcyI GRCGYC 1 cut(s) 827
AfaI GTAC 1 cut(s) 887
AfiI CCNNNNNNNGG 7 cut(s) 121, 204, 579, 580, 607, 735, 783
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 5 cut(s) 21, 57, 213, 523, 796
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 5 cut(s) 53, 385, 552, 742, 810
AluI AGCT 5 cut(s) 53, 385, 552, 742, 810
Alw26I GTCTC 2 cut(s) 339, 407
AlwI GGATC 3 cut(s) 215, 560, 563
ApeKI GCWGC 2 cut(s) 14, 742
ApoI RAATTY 1 cut(s) 589
Asp700I GAANNNNTTC 1 cut(s) 477
AspLEI GCGC 2 cut(s) 140, 158
AspS9I GGNCC 3 cut(s) 88, 464, 726
AsuC2I CCSGG 2 cut(s) 449, 574
AsuHPI GGTGA 5 cut(s) 91, 254, 622, 698, 769
AvaII GGWCC 1 cut(s) 726
BbrPI CACGTG 3 cut(s) 227, 717, 748
BbvI GCAGC 1 cut(s) 729
BccI CCATC 1 cut(s) 78
BceAI ACGGC 5 cut(s) 17, 50, 102, 127, 344
BcnI CCSGG 2 cut(s) 449, 574
BcoDI GTCTC 2 cut(s) 339, 407
BfuAI ACCTGC 2 cut(s) 110, 657
BglI GCCNNNNNGGC 2 cut(s) 111, 131
BisI GCNGC 4 cut(s) 15, 126, 585, 743
BlsI GCNGC 4 cut(s) 16, 127, 586, 744
BmcAI AGTACT 1 cut(s) 887
Bme1390I CCNGG 2 cut(s) 449, 574
Bme18I GGWCC 1 cut(s) 726
BmgT120I GGNCC 3 cut(s) 88, 464, 726
BmiI GGNNCC 2 cut(s) 431, 727
BmrFI CCNGG 2 cut(s) 449, 574
BmrI ACTGGG 1 cut(s) 972
BmsI GCATC 2 cut(s) 149, 416
BmuI ACTGGG 1 cut(s) 972
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpuEI CTTGAG 1 cut(s) 562
BpuMI CCSGG 2 cut(s) 449, 574
Bsa29I ATCGAT 1 cut(s) 564
BsaAI YACGTR 3 cut(s) 227, 717, 748
BsaBI GATNNNNATC 1 cut(s) 560
BsaHI GRCGYC 1 cut(s) 827
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 3 cut(s) 120, 342, 777
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 7 cut(s) 121, 204, 579, 580, 607, 735, 783
Bse118I RCCGGY 3 cut(s) 28, 257, 607
Bse1I ACTGG 1 cut(s) 967
Bse3DI GCAATG 1 cut(s) 954
Bse8I GATNNNNATC 1 cut(s) 560
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 120, 342, 777
BseGI GGATG 2 cut(s) 70, 93
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 7 cut(s) 121, 204, 579, 580, 607, 735, 783
BseMI GCAATG 1 cut(s) 954
BseMII CTCAG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 967
BseRI GAGGAG 5 cut(s) 284, 548, 862, 896, 940
BseX3I CGGCCG 2 cut(s) 30, 357
BseXI GCAGC 1 cut(s) 729
BseYI CCCAGC 1 cut(s) 253
BsgI GTGCAG 4 cut(s) 638, 716, 726, 728
Bsh1236I CGCG 3 cut(s) 156, 344, 659
Bsh1285I CGRYCG 3 cut(s) 33, 360, 834
BshVI ATCGAT 1 cut(s) 564
BsiEI CGRYCG 3 cut(s) 33, 360, 834
BsiSI CCGG 6 cut(s) 29, 258, 448, 501, 574, 608
BslFI GGGAC 2 cut(s) 200, 739
BslI CCNNNNNNNGG 7 cut(s) 121, 204, 579, 580, 607, 735, 783
BsmAI GTCTC 2 cut(s) 339, 407
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 2 cut(s) 200, 739
Bso31I GGTCTC 1 cut(s) 407
Bsp143I GATC 6 cut(s) 220, 555, 561, 565, 831, 948
Bsp19I CCATGG 1 cut(s) 777
Bsp68I TCGCGA 1 cut(s) 659
BspCNI CTCAG 1 cut(s) 336
BspDI ATCGAT 1 cut(s) 564
BspFNI CGCG 3 cut(s) 156, 344, 659
BspLI GGNNCC 2 cut(s) 431, 727
BspMI ACCTGC 2 cut(s) 110, 657
BspPI GGATC 3 cut(s) 215, 560, 563
BspTNI GGTCTC 1 cut(s) 407
BsrBI CCGCTC 2 cut(s) 267, 292
BsrDI GCAATG 1 cut(s) 954
BsrFI RCCGGY 3 cut(s) 28, 257, 607
BsrI ACTGG 1 cut(s) 967
BssAI RCCGGY 3 cut(s) 28, 257, 607
BssECI CCNNGG 3 cut(s) 120, 342, 777
BssMI GATC 6 cut(s) 220, 555, 561, 565, 831, 948
BssNI GRCGYC 1 cut(s) 827
BssT1I CCWWGG 2 cut(s) 120, 777
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 2 cut(s) 839, 893
BstACI GRCGYC 1 cut(s) 827
BstBAI YACGTR 3 cut(s) 227, 717, 748
BstC8I GCNNGC 4 cut(s) 136, 383, 440, 764
BstDEI CTNAG 2 cut(s) 323, 396
BstDSI CCRYGG 2 cut(s) 342, 777
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 2 cut(s) 70, 93
BstFNI CGCG 3 cut(s) 156, 344, 659
BstHHI GCGC 2 cut(s) 140, 158
BstKTI GATC 6 cut(s) 223, 558, 564, 568, 834, 951
BstMAI GTCTC 2 cut(s) 339, 407
BstMBI GATC 6 cut(s) 220, 555, 561, 565, 831, 948
BstMCI CGRYCG 3 cut(s) 33, 360, 834
BstMWI GCNNNNNNNGC 3 cut(s) 111, 131, 164
BstNSI RCATGY 3 cut(s) 500, 766, 770
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 447, 572
BstUI CGCG 3 cut(s) 156, 344, 659
BstV1I GCAGC 1 cut(s) 729
BstZI CGGCCG 2 cut(s) 30, 357
Bsu15I ATCGAT 1 cut(s) 564
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 2 cut(s) 342, 777
BtsCI GGATG 2 cut(s) 70, 93
BtsI GCAGTG 2 cut(s) 704, 714
BtsIMutI CAGTG 3 cut(s) 704, 714, 960
BtuMI TCGCGA 1 cut(s) 659
BveI ACCTGC 2 cut(s) 110, 657
Cac8I GCNNGC 4 cut(s) 136, 383, 440, 764
CfoI GCGC 2 cut(s) 140, 158
Cfr10I RCCGGY 3 cut(s) 28, 257, 607
Cfr13I GGNCC 3 cut(s) 88, 464, 726
Cfr42I CCGCGG 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 1 cut(s) 886
CviAII CATG 6 cut(s) 202, 497, 763, 767, 778, 946
CviQI GTAC 1 cut(s) 886
DdeI CTNAG 2 cut(s) 323, 396
DpnI GATC 6 cut(s) 222, 557, 563, 567, 833, 950
DpnII GATC 6 cut(s) 220, 555, 561, 565, 831, 948
EaeI YGGCCR 4 cut(s) 30, 112, 123, 357
EagI CGGCCG 2 cut(s) 30, 357
Eam1104I CTCTTC 2 cut(s) 839, 893
EarI CTCTTC 2 cut(s) 839, 893
EciI GGCGGA 1 cut(s) 154
EclXI CGGCCG 2 cut(s) 30, 357
Eco130I CCWWGG 2 cut(s) 120, 777
Eco31I GGTCTC 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 726
Eco52I CGGCCG 2 cut(s) 30, 357
Eco72I CACGTG 3 cut(s) 227, 717, 748
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoT14I CCWWGG 2 cut(s) 120, 777
EcoT22I ATGCAT 1 cut(s) 768
ErhI CCWWGG 2 cut(s) 120, 777
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 6 cut(s) 205, 500, 766, 770, 781, 949
FaiI YATR 9 cut(s) 63, 203, 498, 693, 764, 768, 779, 947, 980
FaqI GGGAC 2 cut(s) 200, 739
FatI CATG 6 cut(s) 201, 496, 762, 766, 777, 945
FauI CCCGC 2 cut(s) 177, 297
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 4 cut(s) 15, 126, 585, 743
FokI GGATG 2 cut(s) 57, 80
Fsp4HI GCNGC 4 cut(s) 15, 126, 585, 743
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 2 cut(s) 139, 157
GluI GCNGC 4 cut(s) 15, 126, 585, 743
GsaI CCCAGC 1 cut(s) 257
HapII CCGG 6 cut(s) 29, 258, 448, 501, 574, 608
HhaI GCGC 2 cut(s) 140, 158
Hin1I GRCGYC 1 cut(s) 827
Hin1II CATG 6 cut(s) 205, 500, 766, 770, 781, 949
Hin6I GCGC 2 cut(s) 138, 156
HinP1I GCGC 2 cut(s) 138, 156
HincII GTYRAC 2 cut(s) 37, 232
HindII GTYRAC 2 cut(s) 37, 232
HinfI GANTC 3 cut(s) 281, 419, 787
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 6 cut(s) 29, 258, 448, 501, 574, 608
HphI GGTGA 5 cut(s) 91, 254, 622, 698, 769
Hpy166II GTNNAC 3 cut(s) 37, 232, 454
Hpy188I TCNGA 7 cut(s) 148, 280, 412, 424, 494, 570, 1007
Hpy188III TCNNGA 3 cut(s) 74, 559, 658
Hpy8I GTNNAC 3 cut(s) 37, 232, 454
Hpy99I CGWCG 6 cut(s) 38, 113, 173, 365, 664, 832
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 4 cut(s) 226, 716, 747, 827
HpyCH4V TGCA 8 cut(s) 619, 697, 707, 720, 745, 766, 823, 954
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 131, 164
HpyF3I CTNAG 2 cut(s) 323, 396
HpySE526I ACGT 4 cut(s) 226, 716, 747, 827
Hsp92I GRCGYC 1 cut(s) 827
Hsp92II CATG 6 cut(s) 205, 500, 766, 770, 781, 949
HspAI GCGC 2 cut(s) 138, 156
KspAI GTTAAC 1 cut(s) 232
KspI CCGCGG 1 cut(s) 345
Kzo9I GATC 6 cut(s) 220, 555, 561, 565, 831, 948
LmnI GCTCC 3 cut(s) 297, 739, 849
Lsp1109I GCAGC 1 cut(s) 729
LweI GCATC 2 cut(s) 149, 416
MaeII ACGT 4 cut(s) 226, 716, 747, 827
MaeIII GTNAC 4 cut(s) 260, 610, 712, 967
MalI GATC 6 cut(s) 222, 557, 563, 567, 833, 950
MbiI CCGCTC 2 cut(s) 267, 292
MboI GATC 6 cut(s) 220, 555, 561, 565, 831, 948
MboII GAAGA 4 cut(s) 265, 691, 856, 910
MluCI AATT 2 cut(s) 314, 589
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 4 cut(s) 303, 517, 593, 858
Mph1103I ATGCAT 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 477
MseI TTAA 2 cut(s) 231, 350
MslI CAYNNNNRTG 2 cut(s) 200, 767
MspA1I CMGCKG 2 cut(s) 128, 344
MspI CCGG 6 cut(s) 29, 258, 448, 501, 574, 608
MspR9I CCNGG 2 cut(s) 449, 574
MvnI CGCG 3 cut(s) 156, 344, 659
MwoI GCNNNNNNNGC 3 cut(s) 111, 131, 164
NciI CCSGG 2 cut(s) 449, 574
NcoI CCATGG 1 cut(s) 777
NdeII GATC 6 cut(s) 220, 555, 561, 565, 831, 948
NlaIII CATG 6 cut(s) 205, 500, 766, 770, 781, 949
NlaIV GGNNCC 2 cut(s) 431, 727
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 3 cut(s) 260, 610, 712
NruI TCGCGA 1 cut(s) 659
NsbI TGCGCA 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 3 cut(s) 500, 766, 770
PaeI GCATGC 1 cut(s) 766
PaqCI CACCTGC 2 cut(s) 110, 657
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 477
PfeI GAWTC 2 cut(s) 419, 787
PflMI CCANNNNNTGG 1 cut(s) 121
PkrI GCNGC 4 cut(s) 16, 127, 586, 744
Ple19I CGATCG 1 cut(s) 834
PleI GAGTC 1 cut(s) 275
PmaCI CACGTG 3 cut(s) 227, 717, 748
PmlI CACGTG 3 cut(s) 227, 717, 748
PpsI GAGTC 1 cut(s) 275
Ppu21I YACGTR 3 cut(s) 227, 717, 748
PspCI CACGTG 3 cut(s) 227, 717, 748
PspEI GGTNACC 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 431, 727
PspPI GGNCC 3 cut(s) 88, 464, 726
PvuI CGATCG 1 cut(s) 834
RruI TCGCGA 1 cut(s) 659
RsaI GTAC 1 cut(s) 887
RsaNI GTAC 1 cut(s) 886
RseI CAYNNNNRTG 2 cut(s) 200, 767
SacII CCGCGG 1 cut(s) 345
SalI GTCGAC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 231, 350
SatI GCNGC 4 cut(s) 15, 126, 585, 743
Sau3AI GATC 6 cut(s) 220, 555, 561, 565, 831, 948
Sau96I GGNCC 3 cut(s) 88, 464, 726
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 449, 574
SfaNI GCATC 2 cut(s) 149, 416
SfiI GGCCNNNNNGGCC 1 cut(s) 131
Sfr303I CCGCGG 1 cut(s) 345
SgrBI CCGCGG 1 cut(s) 345
SinI GGWCC 1 cut(s) 726
SmiMI CAYNNNNRTG 2 cut(s) 200, 767
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
SphI GCATGC 1 cut(s) 766
Sse9I AATT 2 cut(s) 314, 589
SspI AATATT 1 cut(s) 819
StyD4I CCNGG 2 cut(s) 447, 572
StyI CCWWGG 2 cut(s) 120, 777
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 4 cut(s) 229, 719, 750, 830
TaqI TCGA 8 cut(s) 36, 108, 297, 363, 564, 596, 830, 834
TaqII GACCGA 2 cut(s) 431, 743
TasI AATT 2 cut(s) 314, 589
TatI WGTACW 1 cut(s) 885
TauI GCSGC 2 cut(s) 128, 587
TfiI GAWTC 2 cut(s) 419, 787
Tru1I TTAA 2 cut(s) 231, 350
Tru9I TTAA 2 cut(s) 231, 350
TscAI CASTG 3 cut(s) 704, 714, 967
TseFI GTSAC 3 cut(s) 260, 610, 712
TseI GCWGC 2 cut(s) 14, 742
Tsp45I GTSAC 3 cut(s) 260, 610, 712
TspDTI ATGAA 3 cut(s) 229, 911, 985
TspRI CASTG 3 cut(s) 704, 714, 967
Van91I CCANNNNNTGG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 726
XapI RAATTY 1 cut(s) 589
XceI RCATGY 3 cut(s) 500, 766, 770
XmiI GTMKAC 1 cut(s) 36
XmnI GAANNNNTTC 1 cut(s) 477
ZraI GACGTC 1 cut(s) 828
ZrmI AGTACT 1 cut(s) 887
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.