MD03G1174000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011266
Physical Location & Seq
Reverse (-)
23805497 .. 23807068
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1174000.v1.1.491

Sequence Viewer

Length: 528 bp
ATGCTTCCCCTGTATTCTCCTTCACTTCACGGTAAACCACCATTGTCACTGCAACCATTTTCTCCCACAAGTTATAAGCTTAAAACATGCATTTCTGAATTGCTAGAGCCTGGCCATGGCATTTTCAGGAAGAAGAACACAAACCCATATCACCATAATTCTTCTTTCTCCTCCTCTTCCTGTATTGTTTGTGCAGTGGAGAACGAGTCGCAGCGGTTTGAGGTAGACCCAGATAAGGCCAGAGAAGCTCTTAAAAATCTTGACCAGCAACTTCAATCCCGCTCCCAAACTAAATTCCGCCCTCCAAAGGAAAAGGCACCGGACGTGACTTTTACAAGAGATCGAACAAAAGATGAGCAAGTTGAAGAATTTTCAGGATCGTTCTTCACAATCACGGCTGCTGCAGTCTTTGCTTTCACCGTCTTCTATAATGTGCTGTTTTACACTGTTATAAAACCAGCCATAGATGGACCGGGGGCAGCTCCATACACAGTTGTTCAAAGAGAAATCCTAAAGGACCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.77

Weight (kDa)

8.39

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01870
fragaria_vesca FvH4_3g28750
malus_domestica MD03G1174000.v1.1 MD11G1192200.v1.1
prunus_persica Prupe.4G250100_v2.0.a1
pyrus_communis pycom03g13160 pycom11g16800
rosa_chinensis RchiOBHm_Chr5g0052301
rosa_laevigata RLG00000034827
rosa_multiflora Rmu_sc0021240.1_g000003 Rmu_sc0028377.1_g000002
rosa_roxburghii Rroxscaffold_1G00028130 Rroxscaffold_1G00028140
rosa_rugosa Rorug05G0273000
rosa_samantha Rh5AG345900 Rh5BG356600 Rh5CG381900 Rh5DG371800
rosa_wichuraiana Rw5G032620 Rw5G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 75, 452
AccB1I GGYRCC 1 cut(s) 316
AccBSI CCGCTC 1 cut(s) 282
AccI GTMKAC 1 cut(s) 225
AciI CCGC 3 cut(s) 214, 280, 298
AclWI GGATC 1 cut(s) 385
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 2 cut(s) 293, 368
AfiI CCNNNNNNNGG 3 cut(s) 116, 235, 307
AgsI TTSAA 3 cut(s) 275, 365, 500
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 3 cut(s) 79, 248, 482
AluI AGCT 3 cut(s) 79, 248, 482
AlwI GGATC 1 cut(s) 385
AoxI GGCC 2 cut(s) 112, 237
ApeKI GCWGC 4 cut(s) 211, 398, 401, 479
ApoI RAATTY 2 cut(s) 293, 368
AspS9I GGNCC 2 cut(s) 470, 517
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 2 cut(s) 143, 409
AvaII GGWCC 2 cut(s) 470, 517
BalI TGGCCA 1 cut(s) 114
BanI GGYRCC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 4 cut(s) 223, 385, 388, 491
BccI CCATC 1 cut(s) 461
BceAI ACGGC 1 cut(s) 411
BciT130I CCWGG 1 cut(s) 111
BcnI CCSGG 1 cut(s) 474
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 402
BisI GCNGC 4 cut(s) 212, 399, 402, 480
BlsI GCNGC 4 cut(s) 213, 400, 403, 481
Bme1390I CCNGG 2 cut(s) 111, 474
Bme18I GGWCC 2 cut(s) 470, 517
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 2 cut(s) 470, 517
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 2 cut(s) 111, 474
BpiI GAAGAC 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 115, 473
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 235, 307
Bse1I ACTGG 1 cut(s) 520
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 115, 473
BseLI CCNNNNNNNGG 3 cut(s) 116, 235, 307
BseNI ACTGG 1 cut(s) 520
BseRI GAGGAG 2 cut(s) 160, 163
BseXI GCAGC 4 cut(s) 223, 385, 388, 491
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 2 cut(s) 114, 239
BshNI GGYRCC 1 cut(s) 316
BsiSI CCGG 2 cut(s) 320, 473
BslI CCNNNNNNNGG 3 cut(s) 116, 235, 307
BsnI GGCC 2 cut(s) 114, 239
Bsp143I GATC 2 cut(s) 340, 377
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 3 cut(s) 214, 280, 298
BspANI GGCC 2 cut(s) 114, 239
BspLI GGNNCC 1 cut(s) 318
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 316
BsrBI CCGCTC 1 cut(s) 282
BsrI ACTGG 1 cut(s) 520
BssECI CCNNGG 2 cut(s) 115, 473
BssMI GATC 2 cut(s) 340, 377
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 4 cut(s) 32, 421, 448, 493
Bst6I CTCTTC 1 cut(s) 181
BstAPI GCANNNNNTGC 1 cut(s) 410
BstDSI CCRYGG 1 cut(s) 115
BstKTI GATC 2 cut(s) 343, 380
BstMBI GATC 2 cut(s) 340, 377
BstMWI GCNNNNNNNGC 2 cut(s) 245, 410
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 109, 472
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 4 cut(s) 223, 385, 388, 491
BstV2I GAAGAC 1 cut(s) 415
BsuRI GGCC 2 cut(s) 114, 239
BtgI CCRYGG 1 cut(s) 115
BtrI CACGTC 1 cut(s) 325
BtsI GCAGTG 2 cut(s) 47, 201
BtsIMutI CAGTG 3 cut(s) 47, 201, 444
Cfr13I GGNCC 2 cut(s) 470, 517
CviAII CATG 2 cut(s) 87, 116
CviJI RGCY 8 cut(s) 79, 109, 114, 239, 248, 398, 461, 482
CviKI_1 RGCY 8 cut(s) 79, 109, 114, 239, 248, 398, 461, 482
DpnI GATC 2 cut(s) 342, 379
DpnII GATC 2 cut(s) 340, 377
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
EciI GGCGGA 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 470, 517
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 115
EcoT22I ATGCAT 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 2 cut(s) 90, 119
FaiI YATR 9 cut(s) 75, 88, 117, 148, 156, 429, 452, 464, 487
FatI CATG 2 cut(s) 86, 115
FauI CCCGC 1 cut(s) 287
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 212, 399, 402, 480
Fsp4HI GCNGC 4 cut(s) 212, 399, 402, 480
FspBI CTAG 1 cut(s) 104
GluI GCNGC 4 cut(s) 212, 399, 402, 480
HaeIII GGCC 2 cut(s) 114, 239
HapII CCGG 2 cut(s) 320, 473
Hin1II CATG 2 cut(s) 90, 119
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 1 cut(s) 206
HpaII CCGG 2 cut(s) 320, 473
HphI GGTGA 2 cut(s) 143, 409
Hpy166II GTNNAC 2 cut(s) 35, 226
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 3 cut(s) 127, 260, 375
Hpy8I GTNNAC 2 cut(s) 35, 226
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 4 cut(s) 32, 421, 448, 493
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 4 cut(s) 52, 90, 194, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 410
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 2 cut(s) 90, 119
Kzo9I GATC 2 cut(s) 340, 377
LmnI GCTCC 2 cut(s) 287, 487
Lsp1109I GCAGC 4 cut(s) 223, 385, 388, 491
MaeI CTAG 1 cut(s) 104
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 45, 325
MalI GATC 2 cut(s) 342, 379
MbiI CCGCTC 1 cut(s) 282
MboI GATC 2 cut(s) 340, 377
MboII GAAGA 7 cut(s) 142, 145, 153, 168, 376, 377, 415
MlsI TGGCCA 1 cut(s) 114
MluCI AATT 4 cut(s) 98, 157, 293, 368
MluNI TGGCCA 1 cut(s) 114
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 4 cut(s) 181, 184, 214, 312
Mox20I TGGCCA 1 cut(s) 114
Mph1103I ATGCAT 1 cut(s) 92
MscI TGGCCA 1 cut(s) 114
MseI TTAA 2 cut(s) 81, 252
Msp20I TGGCCA 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 2 cut(s) 320, 473
MspR9I CCNGG 2 cut(s) 111, 474
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 245, 410
NciI CCSGG 1 cut(s) 474
NcoI CCATGG 1 cut(s) 115
NdeII GATC 2 cut(s) 340, 377
NlaIII CATG 2 cut(s) 90, 119
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 45, 325
NsiI ATGCAT 1 cut(s) 92
NspI RCATGY 1 cut(s) 90
PkrI GCNGC 4 cut(s) 213, 400, 403, 481
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PsiI TTATAA 2 cut(s) 75, 452
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 470, 517
PstI CTGCAG 1 cut(s) 406
SaqAI TTAA 2 cut(s) 81, 252
SatI GCNGC 4 cut(s) 212, 399, 402, 480
Sau3AI GATC 2 cut(s) 340, 377
Sau96I GGNCC 2 cut(s) 470, 517
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 2 cut(s) 111, 474
SetI ASST 5 cut(s) 81, 225, 250, 327, 484
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 2 cut(s) 470, 517
Sse9I AATT 4 cut(s) 98, 157, 293, 368
SsiI CCGC 3 cut(s) 214, 280, 298
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 109, 472
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 4 cut(s) 32, 421, 448, 493
TaiI ACGT 1 cut(s) 327
TaqI TCGA 1 cut(s) 343
TasI AATT 4 cut(s) 98, 157, 293, 368
Tru1I TTAA 2 cut(s) 81, 252
Tru9I TTAA 2 cut(s) 81, 252
TscAI CASTG 3 cut(s) 54, 201, 451
TseFI GTSAC 2 cut(s) 45, 325
TseI GCWGC 4 cut(s) 211, 398, 401, 479
Tsp45I GTSAC 2 cut(s) 45, 325
TspRI CASTG 3 cut(s) 54, 201, 451
VpaK11BI GGWCC 2 cut(s) 470, 517
XapI RAATTY 2 cut(s) 293, 368
XceI RCATGY 1 cut(s) 90
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 104
Zsp2I ATGCAT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.