pycom03g13160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011266
Physical Location & Seq
Reverse (-)
14073600 .. 14075095
1496 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13160.1

Sequence Viewer

Length: 591 bp
ATGAACCGGTTTGGTTCAGTTTTCGGAGTTGTGGCTGTGCTTTCATTTATCCCATTCAAATCAATGCTTCCTCTGTATTCTCCTTCACTTCACGGTAAACCACCATTGTCACTGCAACCATTTTCTCCCACAAGTTATAAGCTTAAAACATGCATTTCTGAATTGCTAGAGCCTGGCTATGGCGTTTTCAGGAATAAGAACACAAACCCATATCACCATAATTCTTCTTCTTCCTCCTCTTCCTGTATTGTTTGTGCAGTGGAGAACGAGTCGCAGCGGTTTGAGGTAGACCCGGATAAGGCCAGAGAAGCTCTTAAAAATCTTGACCAGCAACTTCAATCCCGCTCCCAAACTAAATTCCGCCCTCCAAAGGAAAAGGCACCGGATGTGACTTTTACAAGAGATCGAACAAAAGATGAGCAAGTTGAAGAATTTTCAGGATCGTTCTTCACAATCGCGGCTGCTGCAGTCTTTGCTTTCACCGTCTTCTATAATGTGCTGTTTTACACTGTTATAAAACCAGCCATAGATGGACCGGGGGCAGCTCCATACACAGTTGTTCAAAGAGAAATCCTAAAGGACCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.96

Weight (kDa)

8.72

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01870
fragaria_vesca FvH4_3g28750
malus_domestica MD03G1174000.v1.1 MD11G1192200.v1.1
prunus_persica Prupe.4G250100_v2.0.a1
pyrus_communis pycom03g13160 pycom11g16800
rosa_chinensis RchiOBHm_Chr5g0052301
rosa_laevigata RLG00000034827
rosa_multiflora Rmu_sc0021240.1_g000003 Rmu_sc0028377.1_g000002
rosa_roxburghii Rroxscaffold_1G00028130 Rroxscaffold_1G00028140
rosa_rugosa Rorug05G0273000
rosa_samantha Rh5AG345900 Rh5BG356600 Rh5CG381900 Rh5DG371800
rosa_wichuraiana Rw5G032620 Rw5G032660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 138, 515
AccB1I GGYRCC 1 cut(s) 379
AccBSI CCGCTC 1 cut(s) 345
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 458
AciI CCGC 4 cut(s) 277, 343, 361, 458
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 2 cut(s) 356, 431
AfiI CCNNNNNNNGG 3 cut(s) 179, 298, 370
AgeI ACCGGT 1 cut(s) 6
AgsI TTSAA 4 cut(s) 58, 338, 428, 563
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 3 cut(s) 142, 311, 545
AluI AGCT 3 cut(s) 142, 311, 545
AlwI GGATC 1 cut(s) 448
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 4 cut(s) 274, 461, 464, 542
ApoI RAATTY 2 cut(s) 356, 431
AsiGI ACCGGT 1 cut(s) 6
AspS9I GGNCC 2 cut(s) 533, 580
AsuC2I CCSGG 2 cut(s) 293, 537
AsuHPI GGTGA 2 cut(s) 206, 472
AvaII GGWCC 2 cut(s) 533, 580
BanI GGYRCC 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 478
BbvI GCAGC 4 cut(s) 286, 448, 451, 554
BccI CCATC 1 cut(s) 524
BciT130I CCWGG 1 cut(s) 174
BcnI CCSGG 2 cut(s) 293, 537
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 465
BisI GCNGC 5 cut(s) 275, 459, 462, 465, 543
BlsI GCNGC 5 cut(s) 276, 460, 463, 466, 544
Bme1390I CCNGG 3 cut(s) 174, 293, 537
Bme18I GGWCC 2 cut(s) 533, 580
BmgT120I GGNCC 2 cut(s) 533, 580
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 3 cut(s) 174, 293, 537
BpiI GAAGAC 1 cut(s) 478
BpuMI CCSGG 2 cut(s) 293, 537
BsaJI CCNNGG 1 cut(s) 536
BsaWI WCCGGW 2 cut(s) 6, 382
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 298, 370
Bse118I RCCGGY 1 cut(s) 6
Bse1I ACTGG 1 cut(s) 583
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 536
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 3 cut(s) 179, 298, 370
BseNI ACTGG 1 cut(s) 583
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 4 cut(s) 286, 448, 451, 554
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 458
BshFI GGCC 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 379
BshTI ACCGGT 1 cut(s) 6
BsiSI CCGG 4 cut(s) 7, 293, 383, 536
BslI CCNNNNNNNGG 3 cut(s) 179, 298, 370
BsnI GGCC 1 cut(s) 302
Bsp143I GATC 2 cut(s) 403, 440
BspACI CCGC 4 cut(s) 277, 343, 361, 458
BspANI GGCC 1 cut(s) 302
BspFNI CGCG 1 cut(s) 458
BspLI GGNNCC 1 cut(s) 381
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 1 cut(s) 448
BspT107I GGYRCC 1 cut(s) 379
BsrBI CCGCTC 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 6
BsrI ACTGG 1 cut(s) 583
BssAI RCCGGY 1 cut(s) 6
BssECI CCNNGG 1 cut(s) 536
BssMI GATC 2 cut(s) 403, 440
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 4 cut(s) 95, 484, 511, 556
Bst6I CTCTTC 1 cut(s) 244
BstAPI GCANNNNNTGC 1 cut(s) 473
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 458
BstKTI GATC 2 cut(s) 406, 443
BstMBI GATC 2 cut(s) 403, 440
BstMWI GCNNNNNNNGC 3 cut(s) 308, 464, 473
BstNI CCWGG 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 3 cut(s) 172, 291, 535
BstSFI CTRYAG 1 cut(s) 465
BstUI CGCG 1 cut(s) 458
BstV1I GCAGC 4 cut(s) 286, 448, 451, 554
BstV2I GAAGAC 1 cut(s) 478
BsuRI GGCC 1 cut(s) 302
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 2 cut(s) 110, 264
BtsIMutI CAGTG 3 cut(s) 110, 264, 507
Cfr10I RCCGGY 1 cut(s) 6
Cfr13I GGNCC 2 cut(s) 533, 580
CspAI ACCGGT 1 cut(s) 6
CviAII CATG 1 cut(s) 150
CviJI RGCY 9 cut(s) 35, 142, 172, 177, 302, 311, 461, 524, 545
CviKI_1 RGCY 9 cut(s) 35, 142, 172, 177, 302, 311, 461, 524, 545
DpnI GATC 2 cut(s) 405, 442
DpnII GATC 2 cut(s) 403, 440
Eam1104I CTCTTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 244
EciI GGCGGA 1 cut(s) 350
Eco47I GGWCC 2 cut(s) 533, 580
EcoRII CCWGG 1 cut(s) 172
EcoT22I ATGCAT 1 cut(s) 155
FaeI CATG 1 cut(s) 153
FaiI YATR 9 cut(s) 138, 151, 180, 211, 219, 492, 515, 527, 550
FatI CATG 1 cut(s) 149
FauI CCCGC 1 cut(s) 350
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 5 cut(s) 275, 459, 462, 465, 543
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 5 cut(s) 275, 459, 462, 465, 543
FspBI CTAG 1 cut(s) 167
GluI GCNGC 5 cut(s) 275, 459, 462, 465, 543
HaeIII GGCC 1 cut(s) 302
HapII CCGG 4 cut(s) 7, 293, 383, 536
Hin1II CATG 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 1 cut(s) 269
HpaII CCGG 4 cut(s) 7, 293, 383, 536
HphI GGTGA 2 cut(s) 206, 472
Hpy166II GTNNAC 2 cut(s) 98, 289
Hpy188I TCNGA 2 cut(s) 26, 160
Hpy188III TCNNGA 3 cut(s) 190, 323, 438
Hpy8I GTNNAC 2 cut(s) 98, 289
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 4 cut(s) 95, 484, 511, 556
HpyCH4V TGCA 4 cut(s) 115, 153, 257, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 308, 464, 473
Hsp92II CATG 1 cut(s) 153
Kzo9I GATC 2 cut(s) 403, 440
LmnI GCTCC 2 cut(s) 350, 550
Lsp1109I GCAGC 4 cut(s) 286, 448, 451, 554
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 2 cut(s) 108, 388
MalI GATC 2 cut(s) 405, 442
MbiI CCGCTC 1 cut(s) 345
MboI GATC 2 cut(s) 403, 440
MboII GAAGA 7 cut(s) 216, 219, 222, 231, 439, 440, 478
MluCI AATT 4 cut(s) 161, 220, 356, 431
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 5 cut(s) 81, 244, 247, 277, 375
Mph1103I ATGCAT 1 cut(s) 155
MseI TTAA 2 cut(s) 144, 315
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 4 cut(s) 7, 293, 383, 536
MspR9I CCNGG 3 cut(s) 174, 293, 537
MvaI CCWGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 458
MwoI GCNNNNNNNGC 3 cut(s) 308, 464, 473
NciI CCSGG 2 cut(s) 293, 537
NdeII GATC 2 cut(s) 403, 440
NlaIII CATG 1 cut(s) 153
NlaIV GGNNCC 1 cut(s) 381
NmuCI GTSAC 2 cut(s) 108, 388
NsiI ATGCAT 1 cut(s) 155
NspI RCATGY 1 cut(s) 153
PinAI ACCGGT 1 cut(s) 6
PkrI GCNGC 5 cut(s) 276, 460, 463, 466, 544
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PsiI TTATAA 2 cut(s) 138, 515
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 2 cut(s) 533, 580
PstI CTGCAG 1 cut(s) 469
SaqAI TTAA 2 cut(s) 144, 315
SatI GCNGC 5 cut(s) 275, 459, 462, 465, 543
Sau3AI GATC 2 cut(s) 403, 440
Sau96I GGNCC 2 cut(s) 533, 580
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 3 cut(s) 174, 293, 537
SetI ASST 4 cut(s) 144, 288, 313, 547
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 2 cut(s) 533, 580
Sse9I AATT 4 cut(s) 161, 220, 356, 431
SsiI CCGC 4 cut(s) 277, 343, 361, 458
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 3 cut(s) 172, 291, 535
TaaI ACNGT 4 cut(s) 95, 484, 511, 556
TaqI TCGA 1 cut(s) 406
TasI AATT 4 cut(s) 161, 220, 356, 431
TauI GCSGC 1 cut(s) 461
Tru1I TTAA 2 cut(s) 144, 315
Tru9I TTAA 2 cut(s) 144, 315
TscAI CASTG 3 cut(s) 117, 264, 514
TseFI GTSAC 2 cut(s) 108, 388
TseI GCWGC 4 cut(s) 274, 461, 464, 542
Tsp45I GTSAC 2 cut(s) 108, 388
TspDTI ATGAA 2 cut(s) 17, 33
TspRI CASTG 3 cut(s) 117, 264, 514
VpaK11BI GGWCC 2 cut(s) 533, 580
XapI RAATTY 2 cut(s) 356, 431
XceI RCATGY 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.