Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012741

Physical Location & Seq

Genomic Coordinates Forward (+)

24311076 .. 24320771

Gene Structure 9696 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1176900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGACCCCAAAAAGGTCTCCTCTCTGTCCCCCGTTGAATCTGTGTCTCTTTGTCTCCAAATTCAAAGCCAAAACCGCCGCTGCCGGCGCCGCCTCACATTCCATGGCCATCCCAGCTGTAGTTGCCATCTTACCTGTCGGCGTTCTCTTCATTCTCTCTGGCCTCATTGTTAATCTCATGCAGATAGTGTGCTTCCTCCTTGTTCGTCCTGTATCAAAGAGAATGTATAGAAAGATCAACAAACTAGTAGCAGAATTGCTGTGGTTGGAGCTCATATGGCTCATTGATTGGTGGGCAGGAATCAAGGCATGCTTCTTTCTTAAAGTTAATTCTTTCAGTCGAAGAGAGGGATTTAAGATTTTGATGGCAAAAGAAAGAGAAGTTGAACTTTGTAAAGAACATGCACTTGTCATTTCCAATCACAAAAGTGACATTGATTGGCTTGTCGGATGGCTTGTAGCTCAGCGCTCAGGTTGCCTTGGTAGCGCACTAGCCATCATAAAGAAAGAAATAATGTTCCTCCCAGTCATCGGTTGGTCAATGTGGTTTTCTGAATATGTCTTCTTGCAAAGAAGATGGACAAAAGATGAAAGCACATTGAAGTCAGGTTTTCGAAGACTAGAGGACTTCCCCATGCCTTTTTGGTTGGCTCTCTTTGTAGAAGGAACACGATTTACGCAGGCAAAGTTAATGGCAGCTCAAGAGTTTGCAGCTTCTAGAGGGTTGCCTATTCCTAGGAACGTCTTGCTTCCACGGACGAAGGGGTTTGTTTCATCGGTAATCCATATGCGCTCTTTCATTCCAGCAATTTATGACTGTACACTGGCCGTCCCCAACAATCAGCCACCACCTACATTGTTGAGAATATTCAGAGGGCAATCTTCTGTGTTAAAATTACAAATCAGGAGACACTCAATGCAGGAGTTGCCAGAAACGGCCGATGGAATTGGACAATGGTGTAAAGATGTGTTTGTTACCAAGGATGCCTTATTGGAGAAGTACTTTGCCAAAGGTTCATTCAGTGACCAACAACTTCAAAACATTGGCAGACCTACCAAGTCCTTAATTGTTGTCATACTTTGGTCGATCCTCCTTGCCTATGGAATTTACAAATTTGTGCAATGCTCTGCCCTCCTAGCCTCATGGGAAGGCATTGCATTTTCAGCAACATTCTTGGTCCTTATTGTAATTGTCATGCAAATTCTCATCCAGTCATCTGAGTCAGAGCGTTCTACTGCTCTCACGGTACCCCAAAAAGACCCATCACAGCAGCGACTCATTCAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003841 GO:0008374 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0042171 GO:0071617

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072

Pfam Domains

Protein Families

Acyltransf_C Acyltransferase

Protein Analysis

430

Amino Acids

48.58

Weight (kDa)

9.8

Isoelectric Point (pI)

51.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyltransferase	PF01553	132 - 269	2.2e-14	Acyltransferase
Acyltransf_C	PF16076	288 - 361	1.6e-23	Acyltransferase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012741)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51260
malus_domestica	MD00G1164400.v1.1 MD03G1176900.v1.1
prunus_persica	Prupe.4G245900_v2.0.a1 Prupe.4G245900_v2.0.a1 Prupe.4G245900_v2.0.a1 Prupe.4G245900_v2.0.a1
pyrus_communis	pycom03g13410 pycom11g17030
rosa_chinensis	RchiOBHm_Chr7g0219171
rosa_laevigata	RLG00000002305
rosa_roxburghii	Rroxscaffold_3G00240180
rosa_rugosa	Rorug07G0182800
rosa_samantha	Rh7AG326500 Rh7BG316300 Rh7CG343800 Rh7DG321800
rosa_wichuraiana	Rw7G027500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1246
AccB1I	GGYRCC	2 cut(s)	86, 1246
AciI	CCGC	3 cut(s)	75, 78, 90
AclWI	GGATC	1 cut(s)	1081
AcoI	YGGCCR	3 cut(s)	105, 825, 936
AcsI	RAATTY	4 cut(s)	59, 1104, 1112, 1200
AcyI	GRCGYC	1 cut(s)	87
AfaI	GTAC	3 cut(s)	820, 1001, 1248
AfeI	AGCGCT	1 cut(s)	467
AfiI	CCNNNNNNNGG	2 cut(s)	12, 530
AgsI	TTSAA	5 cut(s)	37, 64, 386, 601, 1037
AhlI	ACTAGT	1 cut(s)	244
AjuI	GAANNNNNNNTTGG	2 cut(s)	1001, 1033
AleI	CACNNNNGTG	1 cut(s)	426
AluBI	AGCT	5 cut(s)	116, 271, 461, 698, 713
AluI	AGCT	5 cut(s)	116, 271, 461, 698, 713
Alw21I	GWGCWC	1 cut(s)	273
Alw26I	GTCTC	4 cut(s)	21, 50, 58, 901
AlwI	GGATC	1 cut(s)	1081
Aor51HI	AGCGCT	1 cut(s)	467
AoxI	GGCC	4 cut(s)	105, 160, 825, 936
ApeKI	GCWGC	4 cut(s)	80, 695, 710, 1270
ApoI	RAATTY	4 cut(s)	59, 1104, 1112, 1200
Asp718I	GGTACC	1 cut(s)	1246
AspA2I	CCTAGG	1 cut(s)	734
AspLEI	GCGC	4 cut(s)	89, 468, 488, 792
AspS9I	GGNCC	1 cut(s)	1177
AsuII	TTCGAA	1 cut(s)	613
AvaII	GGWCC	1 cut(s)	1177
AvrII	CCTAGG	1 cut(s)	734
BalI	TGGCCA	1 cut(s)	107
BanI	GGYRCC	2 cut(s)	86, 1246
BanII	GRGCYC	1 cut(s)	273
BbsI	GAAGAC	2 cut(s)	553, 622
Bbv12I	GWGCWC	1 cut(s)	273
BbvI	GCAGC	4 cut(s)	67, 707, 722, 1282
BccI	CCATC	8 cut(s)	116, 134, 358, 444, 503, 570, 935, 1270
BceAI	ACGGC	2 cut(s)	812, 951
BcoDI	GTCTC	4 cut(s)	21, 50, 58, 901
BcuI	ACTAGT	1 cut(s)	244
BfaI	CTAG	6 cut(s)	245, 491, 620, 717, 735, 1136
BfmI	CTRYAG	1 cut(s)	117
BfoI	RGCGCY	2 cut(s)	90, 469
BisI	GCNGC	6 cut(s)	78, 81, 90, 696, 711, 1271
BlnI	CCTAGG	1 cut(s)	734
BlpI	GCTNAGC	1 cut(s)	462
BlsI	GCNGC	6 cut(s)	79, 82, 91, 697, 712, 1272
BmcAI	AGTACT	1 cut(s)	1001
Bme18I	GGWCC	1 cut(s)	1177
BmgT120I	GGNCC	1 cut(s)	1177
BmiI	GGNNCC	2 cut(s)	88, 1248
BmrI	ACTGGG	1 cut(s)	518
BmsI	GCATC	1 cut(s)	973
BmuI	ACTGGG	1 cut(s)	518
BpiI	GAAGAC	2 cut(s)	553, 622
Bpu10I	CCTNAGC	1 cut(s)	469
Bpu1102I	GCTNAGC	1 cut(s)	462
Bpu14I	TTCGAA	1 cut(s)	613
BpuEI	CTTGAG	1 cut(s)	684
BsaHI	GRCGYC	1 cut(s)	87
BsaI	GGTCTC	1 cut(s)	21
BsaJI	CCNNGG	5 cut(s)	102, 478, 734, 752, 978
Bsc4I	CCNNNNNNNGG	2 cut(s)	12, 530
Bse118I	RCCGGY	1 cut(s)	83
Bse1I	ACTGG	3 cut(s)	524, 828, 1210
Bse3DI	GCAATG	2 cut(s)	1127, 1152
BseDI	CCNNGG	5 cut(s)	102, 478, 734, 752, 978
BseGI	GGATG	4 cut(s)	108, 455, 988, 1206
BseLI	CCNNNNNNNGG	2 cut(s)	12, 530
BseMI	GCAATG	2 cut(s)	1127, 1152
BseMII	CTCAG	3 cut(s)	476, 483, 1209
BseNI	ACTGG	3 cut(s)	524, 828, 1210
BseRI	GAGGAG	1 cut(s)	9
BseX3I	CGGCCG	1 cut(s)	936
BseXI	GCAGC	4 cut(s)	67, 707, 722, 1282
BseYI	CCCAGC	1 cut(s)	112
Bsh1285I	CGRYCG	1 cut(s)	939
BshFI	GGCC	4 cut(s)	107, 162, 827, 938
BshNI	GGYRCC	2 cut(s)	86, 1246
BsiEI	CGRYCG	1 cut(s)	939
BsiHKAI	GWGCWC	1 cut(s)	273
BsiSI	CCGG	1 cut(s)	84
BslFI	GGGAC	2 cut(s)	12, 815
BslI	CCNNNNNNNGG	2 cut(s)	12, 530
BsmAI	GTCTC	4 cut(s)	21, 50, 58, 901
BsmFI	GGGAC	2 cut(s)	12, 815
BsnI	GGCC	4 cut(s)	107, 162, 827, 938
Bso31I	GGTCTC	1 cut(s)	21
Bsp119I	TTCGAA	1 cut(s)	613
Bsp1286I	GDGCHC	1 cut(s)	273
Bsp1407I	TGTACA	1 cut(s)	818
Bsp143I	GATC	2 cut(s)	234, 1086
Bsp1720I	GCTNAGC	1 cut(s)	462
Bsp19I	CCATGG	1 cut(s)	102
BspACI	CCGC	3 cut(s)	75, 78, 90
BspANI	GGCC	4 cut(s)	107, 162, 827, 938
BspCNI	CTCAG	3 cut(s)	475, 482, 1210
BspLI	GGNNCC	2 cut(s)	88, 1248
BspPI	GGATC	1 cut(s)	1081
BspT104I	TTCGAA	1 cut(s)	613
BspT107I	GGYRCC	2 cut(s)	86, 1246
BspTNI	GGTCTC	1 cut(s)	21
BsrDI	GCAATG	2 cut(s)	1127, 1152
BsrFI	RCCGGY	1 cut(s)	83
BsrGI	TGTACA	1 cut(s)	818
BsrI	ACTGG	3 cut(s)	524, 828, 1210
BssAI	RCCGGY	1 cut(s)	83
BssECI	CCNNGG	5 cut(s)	102, 478, 734, 752, 978
BssMI	GATC	2 cut(s)	234, 1086
BssNI	GRCGYC	1 cut(s)	87
BssT1I	CCWWGG	4 cut(s)	102, 478, 734, 978
Bst4CI	ACNGT	2 cut(s)	818, 1246
Bst6I	CTCTTC	2 cut(s)	152, 337
BstACI	GRCGYC	1 cut(s)	87
BstAPI	GCANNNNNTGC	1 cut(s)	925
BstAUI	TGTACA	1 cut(s)	818
BstBI	TTCGAA	1 cut(s)	613
BstC8I	GCNNGC	3 cut(s)	85, 310, 681
BstDEI	CTNAG	3 cut(s)	462, 469, 1218
BstDSI	CCRYGG	2 cut(s)	102, 752
BstF5I	GGATG	4 cut(s)	108, 455, 988, 1206
BstH2I	RGCGCY	2 cut(s)	90, 469
BstHHI	GCGC	4 cut(s)	89, 468, 488, 792
BstKTI	GATC	2 cut(s)	237, 1089
BstMAI	GTCTC	4 cut(s)	21, 50, 58, 901
BstMBI	GATC	2 cut(s)	234, 1086
BstMCI	CGRYCG	1 cut(s)	939
BstNSI	RCATGY	2 cut(s)	312, 404
BstSFI	CTRYAG	1 cut(s)	117
BstV1I	GCAGC	4 cut(s)	67, 707, 722, 1282
BstV2I	GAAGAC	2 cut(s)	553, 622
BstZI	CGGCCG	1 cut(s)	936
BsuRI	GGCC	4 cut(s)	107, 162, 827, 938
BtgI	CCRYGG	2 cut(s)	102, 752
BtsCI	GGATG	4 cut(s)	108, 455, 988, 1206
BtsIMutI	CAGTG	2 cut(s)	821, 1027
Cac8I	GCNNGC	3 cut(s)	85, 310, 681
CfoI	GCGC	4 cut(s)	89, 468, 488, 792
Cfr10I	RCCGGY	1 cut(s)	83
Cfr13I	GGNCC	1 cut(s)	1177
Csp6I	GTAC	3 cut(s)	819, 1000, 1247
CspCI	CAANNNNNGTGG	2 cut(s)	837, 872
CviAII	CATG	7 cut(s)	103, 178, 309, 401, 634, 1143, 1195
CviQI	GTAC	3 cut(s)	819, 1000, 1247
DdeI	CTNAG	3 cut(s)	462, 469, 1218
DinI	GGCGCC	1 cut(s)	88
DpnI	GATC	2 cut(s)	236, 1088
DpnII	GATC	2 cut(s)	234, 1086
EaeI	YGGCCR	3 cut(s)	105, 825, 936
EagI	CGGCCG	1 cut(s)	936
Eam1104I	CTCTTC	2 cut(s)	152, 337
EarI	CTCTTC	2 cut(s)	152, 337
Ecl136II	GAGCTC	1 cut(s)	271
EclXI	CGGCCG	1 cut(s)	936
Eco130I	CCWWGG	4 cut(s)	102, 478, 734, 978
Eco24I	GRGCYC	1 cut(s)	273
Eco31I	GGTCTC	1 cut(s)	21
Eco47I	GGWCC	1 cut(s)	1177
Eco47III	AGCGCT	1 cut(s)	467
Eco52I	CGGCCG	1 cut(s)	936
Eco53kI	GAGCTC	1 cut(s)	271
EcoICRI	GAGCTC	1 cut(s)	271
EcoT14I	CCWWGG	4 cut(s)	102, 478, 734, 978
EcoT38I	GRGCYC	1 cut(s)	273
EgeI	GGCGCC	1 cut(s)	88
EheI	GGCGCC	1 cut(s)	88
ErhI	CCWWGG	4 cut(s)	102, 478, 734, 978
FaeI	CATG	7 cut(s)	106, 181, 312, 404, 637, 1146, 1198
FalI	AAGNNNNNCTT	6 cut(s)	296, 328, 372, 404, 971, 1003
FaqI	GGGAC	2 cut(s)	12, 815
FatI	CATG	7 cut(s)	102, 177, 308, 400, 633, 1142, 1194
FauNDI	CATATG	2 cut(s)	275, 786
Fnu4HI	GCNGC	6 cut(s)	78, 81, 90, 696, 711, 1271
FokI	GGATG	4 cut(s)	95, 462, 995, 1193
FriOI	GRGCYC	1 cut(s)	273
Fsp4HI	GCNGC	6 cut(s)	78, 81, 90, 696, 711, 1271
FspBI	CTAG	6 cut(s)	245, 491, 620, 717, 735, 1136
GlaI	GCGC	4 cut(s)	88, 467, 487, 791
GluI	GCNGC	6 cut(s)	78, 81, 90, 696, 711, 1271
GsaI	CCCAGC	1 cut(s)	116
HaeII	RGCGCY	2 cut(s)	90, 469
HaeIII	GGCC	4 cut(s)	107, 162, 827, 938
HapII	CCGG	1 cut(s)	84
HhaI	GCGC	4 cut(s)	89, 468, 488, 792
Hin1I	GRCGYC	1 cut(s)	87
Hin1II	CATG	7 cut(s)	106, 181, 312, 404, 637, 1146, 1198
Hin6I	GCGC	4 cut(s)	87, 466, 486, 790
HinP1I	GCGC	4 cut(s)	87, 466, 486, 790
HinfI	GANTC	4 cut(s)	37, 300, 1220, 1275
HpaII	CCGG	1 cut(s)	84
Hpy166II	GTNNAC	1 cut(s)	821
Hpy188I	TCNGA	6 cut(s)	449, 553, 872, 1219, 1225, 1284
Hpy188III	TCNNGA	3 cut(s)	701, 717, 904
Hpy8I	GTNNAC	1 cut(s)	821
HpyAV	CCTTC	3 cut(s)	656, 754, 1142
HpyCH4III	ACNGT	2 cut(s)	818, 1246
HpyCH4IV	ACGT	1 cut(s)	741
HpyCH4V	TGCA	8 cut(s)	181, 404, 568, 710, 919, 1120, 1157, 1198
HpyF3I	CTNAG	3 cut(s)	462, 469, 1218
HpySE526I	ACGT	1 cut(s)	741
Hsp92I	GRCGYC	1 cut(s)	87
Hsp92II	CATG	7 cut(s)	106, 181, 312, 404, 637, 1146, 1198
HspAI	GCGC	4 cut(s)	87, 466, 486, 790
KasI	GGCGCC	1 cut(s)	86
KpnI	GGTACC	1 cut(s)	1250
KroI	GCCGGC	1 cut(s)	83
KroNI	GCCGGC	1 cut(s)	85
Kzo9I	GATC	2 cut(s)	234, 1086
LmnI	GCTCC	1 cut(s)	268
Lsp1109I	GCAGC	4 cut(s)	67, 707, 722, 1282
LweI	GCATC	1 cut(s)	973
MaeI	CTAG	6 cut(s)	245, 491, 620, 717, 735, 1136
MaeII	ACGT	1 cut(s)	741
MaeIII	GTNAC	3 cut(s)	428, 973, 1022
MalI	GATC	2 cut(s)	236, 1088
MboI	GATC	2 cut(s)	234, 1086
MboII	GAAGA	6 cut(s)	139, 354, 553, 585, 627, 873
MhlI	GDGCHC	1 cut(s)	273
MlsI	TGGCCA	1 cut(s)	107
MluNI	TGGCCA	1 cut(s)	107
Mly113I	GGCGCC	1 cut(s)	87
MlyI	GAGTC	2 cut(s)	1229, 1269
MmeI	TCCRAC	2 cut(s)	246, 427
Mox20I	TGGCCA	1 cut(s)	107
MroNI	GCCGGC	1 cut(s)	83
MscI	TGGCCA	1 cut(s)	107
MseI	TTAA	7 cut(s)	171, 321, 327, 354, 689, 890, 1064
MslI	CAYNNNNRTG	1 cut(s)	426
Msp20I	TGGCCA	1 cut(s)	107
MspA1I	CMGCKG	2 cut(s)	80, 116
MspI	CCGG	1 cut(s)	84
NaeI	GCCGGC	1 cut(s)	85
NarI	GGCGCC	1 cut(s)	87
NcoI	CCATGG	1 cut(s)	102
NdeI	CATATG	2 cut(s)	275, 786
NdeII	GATC	2 cut(s)	234, 1086
NgoMIV	GCCGGC	1 cut(s)	83
NlaIII	CATG	7 cut(s)	106, 181, 312, 404, 637, 1146, 1198
NlaIV	GGNNCC	2 cut(s)	88, 1248
NmuCI	GTSAC	2 cut(s)	428, 1022
NspI	RCATGY	2 cut(s)	312, 404
NspV	TTCGAA	1 cut(s)	613
OliI	CACNNNNGTG	1 cut(s)	426
PaeI	GCATGC	1 cut(s)	312
PdiI	GCCGGC	1 cut(s)	85
PfeI	GAWTC	2 cut(s)	37, 300
PkrI	GCNGC	6 cut(s)	79, 82, 91, 697, 712, 1272
PleI	GAGTC	2 cut(s)	1228, 1269
PluTI	GGCGCC	1 cut(s)	90
PpsI	GAGTC	2 cut(s)	1228, 1269
Psp124BI	GAGCTC	1 cut(s)	273
PspFI	CCCAGC	1 cut(s)	112
PspN4I	GGNNCC	2 cut(s)	88, 1248
PspPI	GGNCC	1 cut(s)	1177
PsrI	GAACNNNNNNTAC	2 cut(s)	658, 690
PvuII	CAGCTG	1 cut(s)	116
RsaI	GTAC	3 cut(s)	820, 1001, 1248
RsaNI	GTAC	3 cut(s)	819, 1000, 1247
RseI	CAYNNNNRTG	1 cut(s)	426
SacI	GAGCTC	1 cut(s)	273
SaqAI	TTAA	7 cut(s)	171, 321, 327, 354, 689, 890, 1064
SatI	GCNGC	6 cut(s)	78, 81, 90, 696, 711, 1271
Sau3AI	GATC	2 cut(s)	234, 1086
Sau96I	GGNCC	1 cut(s)	1177
ScaI	AGTACT	1 cut(s)	1001
SchI	GAGTC	2 cut(s)	1229, 1269
SduI	GDGCHC	1 cut(s)	273
SfaNI	GCATC	1 cut(s)	973
SfcI	CTRYAG	1 cut(s)	117
SfoI	GGCGCC	1 cut(s)	88
SfuI	TTCGAA	1 cut(s)	613
SinI	GGWCC	1 cut(s)	1177
SmiMI	CAYNNNNRTG	1 cut(s)	426
SmlI	CTYRAG	1 cut(s)	699
SmoI	CTYRAG	1 cut(s)	699
SpeI	ACTAGT	1 cut(s)	244
SphI	GCATGC	1 cut(s)	312
SsiI	CCGC	3 cut(s)	75, 78, 90
SspDI	GGCGCC	1 cut(s)	86
SspI	AATATT	1 cut(s)	867
SspMI	CTAG	6 cut(s)	245, 491, 620, 717, 735, 1136
SstI	GAGCTC	1 cut(s)	273
StyI	CCWWGG	4 cut(s)	102, 478, 734, 978
TaaI	ACNGT	2 cut(s)	818, 1246
TaiI	ACGT	1 cut(s)	744
TaqI	TCGA	3 cut(s)	340, 613, 1085
TatI	WGTACW	2 cut(s)	818, 999
TauI	GCSGC	2 cut(s)	80, 92
TfiI	GAWTC	2 cut(s)	37, 300
Tru1I	TTAA	7 cut(s)	171, 321, 327, 354, 689, 890, 1064
Tru9I	TTAA	7 cut(s)	171, 321, 327, 354, 689, 890, 1064
TscAI	CASTG	2 cut(s)	828, 1027
TseFI	GTSAC	2 cut(s)	428, 1022
TseI	GCWGC	4 cut(s)	80, 695, 710, 1270
Tsp45I	GTSAC	2 cut(s)	428, 1022
TspDTI	ATGAA	5 cut(s)	139, 603, 762, 787, 1005
TspGWI	ACGGA	1 cut(s)	769
TspRI	CASTG	2 cut(s)	828, 1027
VpaK11BI	GGWCC	1 cut(s)	1177
XapI	RAATTY	4 cut(s)	59, 1104, 1112, 1200
XbaI	TCTAGA	1 cut(s)	716
XceI	RCATGY	2 cut(s)	312, 404
XcmI	CCANNNNNNNNNTGG	1 cut(s)	531
XmaJI	CCTAGG	1 cut(s)	734
XspI	CTAG	6 cut(s)	245, 491, 620, 717, 735, 1136
ZrmI	AGTACT	1 cut(s)	1001

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.