Rroxscaffold_3G00240180

1-acyl-sn-glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012741
Physical Location & Seq
Reverse (-)
31843709 .. 31848588
4880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00240180.1

Sequence Viewer

Length: 1080 bp
ATGGCGATCCCAGCTGTAGTGGCTATTGTTCCGGTGGGCATATTGTTCATTCTCTCAGGCCTCATTGTTAATCTCATGCAGGCAGTTCTTTTCATCCTTATTCGTCCTATATCAAAGAAATTGTACAGAAAGATCAACAAAGTAGTTGCAGAATTGCTGTGGTTGGAACTCATATGGCTAATTGATTGGTGGGCGGGCATCAAGGTTGAGTTGTATGTAGACTCAGAAACTTTTAAATTAATGGGCAAAGAACATGCACTTCTCATTTGTAATCACAGAAGTGACATTGATTGGCTTGTTGGATGGGTCATGGCTCAGCGCTCGGGTTGCCTTGGTAGCGCACTGGCCATCATGAAGAAAGAAGCAATGTTCCTCCCAAGTTCTGCAATACAGTCAGGTTTTCGACGGCTAGAGGATTTCCCTATGCCATTTTGGTTGGCTCTCTTTGTAGAAGGAACACGGTTTACACAGTCAAAGCTAATGGCAGCTCAAGAGTATGCTGCTTCAAGAGGATTGCCTATTCCTAGGAATGTCTTGCTTCCACGTACGAAGGGGTTTGTTTCATCAGTAAGTCATATGCGCTCATTTGTTCCAGCAATTTATGATTGTACATTGGCTATCCCCAACAATCAGCCACCACCTACACTATTGAGAATGTTTAGAGGTCAATCTTCTGTGATAAAATTACAAATCAGGAGACATCCAATGCAAGACTTACCAGAAACAGGGGACGGAATTGGACAGTGGTGTAAAGATGTATTTGTTACCAAGGATGCCTTATTGGAGAAGTATTATACTAAAGCCACATTCAGTGAACTGCAACTTCAAAACATTGGCAGACCTATAAAGTCCTTGCTTGTTGTCATAATTTGGTCTTGCCTCCTCGGCTATGGAATTTTCAAGTTCATTCAGTGGTCTTCCCTACTATCCACATGGGAAGGCATTGCATTTTCAGTGACTTTCTTGGTCCTTGTTGTAATTCTTATGCAAATCCTCATCCAGTCATCTGACTCGGAGCGTTCTACACCTCTCAAGGTGCCCCCAAAAGACTCGGTGACGGAGCAACTCATTCAACAATGA

Protein Analysis

359

Amino Acids

40.61

Weight (kDa)

9.4

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransf_C PF16076 218 - 291 2.2e-23 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1036
AccI GTMKAC 1 cut(s) 219
AciI CCGC 1 cut(s) 194
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 894
AfaI GTAC 3 cut(s) 125, 547, 610
AfeI AGCGCT 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 725
AgsI TTSAA 4 cut(s) 507, 827, 901, 1073
AleI CACNNNNGTG 1 cut(s) 279
AluBI AGCT 3 cut(s) 14, 478, 488
AluI AGCT 3 cut(s) 14, 478, 488
Alw26I GTCTC 1 cut(s) 691
Ama87I CYCGRG 1 cut(s) 322
Aor51HI AGCGCT 1 cut(s) 320
AoxI GGCC 2 cut(s) 58, 345
ApeKI GCWGC 2 cut(s) 485, 500
ApoI RAATTY 1 cut(s) 894
AseI ATTAAT 1 cut(s) 239
AspA2I CCTAGG 1 cut(s) 524
AspLEI GCGC 3 cut(s) 321, 341, 582
AspS9I GGNCC 1 cut(s) 967
AsuHPI GGTGA 1 cut(s) 1066
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 1 cut(s) 967
AvrII CCTAGG 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 1041
BalI TGGCCA 1 cut(s) 347
BanI GGYRCC 1 cut(s) 1036
BbsI GAAGAC 1 cut(s) 909
BbvI GCAGC 2 cut(s) 487, 497
BccI CCATC 2 cut(s) 297, 356
BceAI ACGGC 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 2 cut(s) 410, 525
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 322
BglI GCCNNNNNGGC 1 cut(s) 885
BisI GCNGC 2 cut(s) 486, 501
BlnI CCTAGG 1 cut(s) 524
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 2 cut(s) 487, 502
Bme18I GGWCC 1 cut(s) 967
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 967
BmiI GGNNCC 1 cut(s) 1038
BmsI GCATC 2 cut(s) 207, 763
BpiI GAAGAC 1 cut(s) 909
Bpu1102I GCTNAGC 1 cut(s) 315
BpuEI CTTGAG 2 cut(s) 474, 1016
BsaAI YACGTR 1 cut(s) 545
BsaJI CCNNGG 4 cut(s) 331, 524, 768, 883
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse1I ACTGG 2 cut(s) 348, 1000
Bse3DI GCAATG 2 cut(s) 372, 942
BseDI CCNNGG 4 cut(s) 331, 524, 768, 883
BseGI GGATG 5 cut(s) 93, 308, 700, 778, 996
BseLI CCNNNNNNNGG 1 cut(s) 725
BseMI GCAATG 2 cut(s) 372, 942
BseMII CTCAG 3 cut(s) 69, 237, 329
BseNI ACTGG 2 cut(s) 348, 1000
BseRI GAGGAG 1 cut(s) 872
BseSI GKGCMC 1 cut(s) 1041
BseXI GCAGC 2 cut(s) 487, 497
BseYI CCCAGC 1 cut(s) 10
BshFI GGCC 2 cut(s) 60, 347
BshNI GGYRCC 1 cut(s) 1036
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 1 cut(s) 32
BsiWI CGTACG 1 cut(s) 545
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 1 cut(s) 725
BsmAI GTCTC 1 cut(s) 691
BsmFI GGGAC 1 cut(s) 743
BsnI GGCC 2 cut(s) 60, 347
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 1041
Bsp1407I TGTACA 2 cut(s) 123, 608
Bsp143I GATC 2 cut(s) 6, 132
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 1 cut(s) 194
BspANI GGCC 2 cut(s) 60, 347
BspCNI CTCAG 3 cut(s) 68, 236, 328
BspHI TCATGA 1 cut(s) 351
BspLI GGNNCC 1 cut(s) 1038
BspT107I GGYRCC 1 cut(s) 1036
BsrDI GCAATG 2 cut(s) 372, 942
BsrGI TGTACA 2 cut(s) 123, 608
BsrI ACTGG 2 cut(s) 348, 1000
BssECI CCNNGG 4 cut(s) 331, 524, 768, 883
BssMI GATC 2 cut(s) 6, 132
BssT1I CCWWGG 3 cut(s) 331, 524, 768
Bst4CI ACNGT 4 cut(s) 393, 462, 471, 744
BstAUI TGTACA 2 cut(s) 123, 608
BstBAI YACGTR 1 cut(s) 545
BstC8I GCNNGC 2 cut(s) 81, 196
BstDEI CTNAG 3 cut(s) 55, 223, 315
BstF5I GGATG 5 cut(s) 93, 308, 700, 778, 996
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 3 cut(s) 321, 341, 582
BstKTI GATC 2 cut(s) 9, 135
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 2 cut(s) 6, 132
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 327, 336, 885
BstNSI RCATGY 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 1041
BstV1I GCAGC 2 cut(s) 487, 497
BstV2I GAAGAC 1 cut(s) 909
BsuRI GGCC 2 cut(s) 60, 347
BtsCI GGATG 5 cut(s) 93, 308, 700, 778, 996
BtsIMutI CAGTG 5 cut(s) 341, 749, 817, 917, 960
Cac8I GCNNGC 2 cut(s) 81, 196
CciI TCATGA 1 cut(s) 351
CfoI GCGC 3 cut(s) 321, 341, 582
Cfr13I GGNCC 1 cut(s) 967
Csp6I GTAC 3 cut(s) 124, 546, 609
CviAII CATG 5 cut(s) 76, 254, 310, 352, 933
CviQI GTAC 3 cut(s) 124, 546, 609
DdeI CTNAG 3 cut(s) 55, 223, 315
DpnI GATC 2 cut(s) 8, 134
DpnII GATC 2 cut(s) 6, 132
DraI TTTAAA 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 345
Eco130I CCWWGG 3 cut(s) 331, 524, 768
Eco147I AGGCCT 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 967
Eco47III AGCGCT 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 322
EcoT14I CCWWGG 3 cut(s) 331, 524, 768
ErhI CCWWGG 3 cut(s) 331, 524, 768
FaeI CATG 5 cut(s) 79, 257, 313, 355, 936
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 1 cut(s) 743
FatI CATG 5 cut(s) 75, 253, 309, 351, 932
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 2 cut(s) 173, 576
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 486, 501
FokI GGATG 5 cut(s) 80, 315, 687, 785, 983
Fsp4HI GCNGC 2 cut(s) 486, 501
FspBI CTAG 2 cut(s) 410, 525
GlaI GCGC 3 cut(s) 320, 340, 581
GluI GCNGC 2 cut(s) 486, 501
GsaI CCCAGC 1 cut(s) 14
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 2 cut(s) 60, 347
HapII CCGG 1 cut(s) 32
HhaI GCGC 3 cut(s) 321, 341, 582
Hin1II CATG 5 cut(s) 79, 257, 313, 355, 936
Hin6I GCGC 3 cut(s) 319, 339, 580
HinP1I GCGC 3 cut(s) 319, 339, 580
HinfI GANTC 3 cut(s) 221, 1010, 1049
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 1066
Hpy166II GTNNAC 3 cut(s) 220, 465, 815
Hpy188I TCNGA 3 cut(s) 226, 1009, 1015
Hpy188III TCNNGA 4 cut(s) 352, 491, 507, 694
Hpy8I GTNNAC 3 cut(s) 220, 465, 815
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 3 cut(s) 446, 544, 932
HpyCH4III ACNGT 4 cut(s) 393, 462, 471, 744
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 8 cut(s) 79, 149, 257, 386, 709, 820, 947, 988
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 327, 336, 885
HpyF3I CTNAG 3 cut(s) 55, 223, 315
HpySE526I ACGT 1 cut(s) 544
Hsp92II CATG 5 cut(s) 79, 257, 313, 355, 936
HspAI GCGC 3 cut(s) 319, 339, 580
Kzo9I GATC 2 cut(s) 6, 132
LmnI GCTCC 2 cut(s) 1015, 1060
Lsp1109I GCAGC 2 cut(s) 487, 497
LweI GCATC 2 cut(s) 207, 763
MaeI CTAG 2 cut(s) 410, 525
MaeII ACGT 1 cut(s) 544
MaeIII GTNAC 4 cut(s) 281, 763, 955, 1054
MalI GATC 2 cut(s) 8, 134
MboI GATC 2 cut(s) 6, 132
MboII GAAGA 3 cut(s) 367, 663, 909
MhlI GDGCHC 1 cut(s) 1041
MlsI TGGCCA 1 cut(s) 347
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 3 cut(s) 215, 1004, 1043
MmeI TCCRAC 2 cut(s) 144, 280
MnlI CCTC 9 cut(s) 71, 383, 406, 503, 656, 890, 893, 1004, 1038
Mox20I TGGCCA 1 cut(s) 347
MscI TGGCCA 1 cut(s) 347
MseI TTAA 3 cut(s) 69, 234, 239
MslI CAYNNNNRTG 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 1 cut(s) 32
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 327, 336, 885
NdeI CATATG 2 cut(s) 173, 576
NdeII GATC 2 cut(s) 6, 132
NlaIII CATG 5 cut(s) 79, 257, 313, 355, 936
NlaIV GGNNCC 1 cut(s) 1038
NmeAIII GCCGAG 1 cut(s) 864
NmuCI GTSAC 3 cut(s) 281, 955, 1054
NspI RCATGY 1 cut(s) 257
OliI CACNNNNGTG 1 cut(s) 279
PagI TCATGA 1 cut(s) 351
PceI AGGCCT 1 cut(s) 60
Pfl23II CGTACG 1 cut(s) 545
PkrI GCNGC 2 cut(s) 487, 502
PleI GAGTC 3 cut(s) 215, 1004, 1043
PpsI GAGTC 3 cut(s) 215, 1004, 1043
Ppu21I YACGTR 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 10
PspLI CGTACG 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 1038
PspPI GGNCC 1 cut(s) 967
PsrI GAACNNNNNNTAC 2 cut(s) 448, 480
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 3 cut(s) 125, 547, 610
RsaNI GTAC 3 cut(s) 124, 546, 609
RseI CAYNNNNRTG 1 cut(s) 279
SaqAI TTAA 3 cut(s) 69, 234, 239
SatI GCNGC 2 cut(s) 486, 501
Sau3AI GATC 2 cut(s) 6, 132
Sau96I GGNCC 1 cut(s) 967
SchI GAGTC 3 cut(s) 215, 1004, 1043
SduI GDGCHC 1 cut(s) 1041
SfaNI GCATC 2 cut(s) 207, 763
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 1 cut(s) 967
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 2 cut(s) 489, 1031
SmoI CTYRAG 2 cut(s) 489, 1031
SseBI AGGCCT 1 cut(s) 60
SsiI CCGC 1 cut(s) 194
SspMI CTAG 2 cut(s) 410, 525
StuI AGGCCT 1 cut(s) 60
StyI CCWWGG 3 cut(s) 331, 524, 768
TaaI ACNGT 4 cut(s) 393, 462, 471, 744
TaiI ACGT 1 cut(s) 547
TaqI TCGA 1 cut(s) 403
TatI WGTACW 2 cut(s) 123, 608
Tru1I TTAA 3 cut(s) 69, 234, 239
Tru9I TTAA 3 cut(s) 69, 234, 239
TscAI CASTG 5 cut(s) 348, 749, 817, 917, 960
TseFI GTSAC 3 cut(s) 281, 955, 1054
TseI GCWGC 2 cut(s) 485, 500
Tsp45I GTSAC 3 cut(s) 281, 955, 1054
TspDTI ATGAA 5 cut(s) 37, 82, 368, 552, 895
TspGWI ACGGA 2 cut(s) 747, 1073
TspRI CASTG 5 cut(s) 348, 749, 817, 917, 960
VpaK11BI GGWCC 1 cut(s) 967
VspI ATTAAT 1 cut(s) 239
XapI RAATTY 1 cut(s) 894
XceI RCATGY 1 cut(s) 257
XmaJI CCTAGG 1 cut(s) 524
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 2 cut(s) 410, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.