MD03G1187000.v1.1

30S ribosomal protein S10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010010
Physical Location & Seq
Forward (+)
25558878 .. 25561938
3061 bp
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UTR
Exon/CDS
Intron
MD03G1187000.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGCAGTTTCTTCTATGTCTTCAATCCTAATTCCTTCACTTCCTCTCTCCAATTCTTCTGCATTTCCGACTTCCAAGCCAAAGCTTTCTTCTTTCGCTTTGCCCTCGGCCAACAATGCCTTAAAGCTCCGAGCTTCAAGACCCTCACAGTCATCCACTAGGGTTTATGCCGCTCCTGAAGTCTTGGAGTCCCAGGAAACCCTAATCCCACCTCCAGAAACCCTCGAGGTTGGAAGTTCTGAGACCCCGAGTACTTCATCACTCAGTATTGGTGAAGATGGAGATAAGGCCTCACCCAAGCAGAAAATCAGGATTAAACTGAGGTCTTACTTTGTGCCATTGATAGAAGATTCCTGCAAGCAGATAATGGATGCTGCTAGGAACACGAATGCGAAAACAATGGGTCCTGTACCCTTGCCAACTAAAAAGCGAATCTACTGTGTTCTCAAATCTCCCCATGTCCACAAGGATGCAAGGTTCCATTTTGAAATTCGTACCCACCAACGCCTCATTGATATTCTATACCCAACTGCACAAACAATAGATTCCTTAATGCAACTTGACCTTCCTGCTGGAGTTGATGTGGAGGTCAAACTGTGA

Protein Analysis

200

Amino Acids

21.75

Weight (kDa)

9.25

Isoelectric Point (pI)

63.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S10 PF00338 104 - 198 1.4e-33 Ribosomal protein S10p/S20e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13120 AT3G13120 AT3G13120
fragaria_vesca FvH4_3g29500
malus_domestica MD03G1187000.v1.1 MD11G1202000.v1.1
prunus_persica Prupe.4G239600_v2.0.a1
pyrus_communis pycom03g13930 pycom11g17450
rosa_chinensis RchiOBHm_Chr5g0054381
rosa_laevigata RLG00000034928
rosa_multiflora Rmu_sc0005705.1_g000022
rosa_roxburghii Rroxscaffold_1G00026490
rosa_rugosa Rorug05G0286000 Rorug05G0286000
rosa_samantha Rh5AG356500 Rh5BG368100 Rh5CG391400 Rh5DG382300
rosa_wichuraiana Rw5G033490 Rw5G033660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 224
AccBSI CCGCTC 1 cut(s) 173
AciI CCGC 1 cut(s) 171
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 489
AcuI CTGAAG 1 cut(s) 198
AfaI GTAC 3 cut(s) 253, 411, 496
AgsI TTSAA 3 cut(s) 24, 138, 488
AjnI CCWGG 1 cut(s) 192
AjuI GAANNNNNNNTTGG 2 cut(s) 73, 105
AloI GAACNNNNNNTCC 2 cut(s) 461, 493
AluBI AGCT 3 cut(s) 85, 127, 134
AluI AGCT 3 cut(s) 85, 127, 134
Alw26I GTCTC 1 cut(s) 236
Ama87I CYCGRG 2 cut(s) 224, 247
AoxI GGCC 2 cut(s) 108, 288
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 1 cut(s) 489
AspS9I GGNCC 1 cut(s) 404
AsuHPI GGTGA 2 cut(s) 284, 285
AvaI CYCGRG 2 cut(s) 224, 247
AvaII GGWCC 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 361
BccI CCATC 1 cut(s) 272
BciT130I CCWGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 2 cut(s) 159, 378
BisI GCNGC 2 cut(s) 171, 375
BlsI GCNGC 2 cut(s) 172, 376
BmcAI AGTACT 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 1 cut(s) 404
BmeT110I CYCGRG 2 cut(s) 224, 247
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 2 cut(s) 405, 479
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 361, 460
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 2 cut(s) 198, 594
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 105, 192
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 105, 192
BseGI GGATG 3 cut(s) 152, 376, 475
BseMII CTCAG 3 cut(s) 231, 277, 311
BseXI GCAGC 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 516
BshFI GGCC 2 cut(s) 110, 290
BsiHKCI CYCGRG 2 cut(s) 224, 247
BslFI GGGAC 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 2 cut(s) 110, 290
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 2 cut(s) 224, 247
BspACI CCGC 1 cut(s) 171
BspANI GGCC 2 cut(s) 110, 290
BspCNI CTCAG 3 cut(s) 232, 276, 312
BspLI GGNNCC 2 cut(s) 405, 479
BspTNI GGTCTC 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 105, 192
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 150, 440, 597
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 3 cut(s) 240, 263, 320
BstF5I GGATG 3 cut(s) 152, 376, 475
BstMAI GTCTC 1 cut(s) 236
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 1 cut(s) 12
BsuRI GGCC 2 cut(s) 110, 290
BtsCI GGATG 3 cut(s) 152, 376, 475
Cac8I GCNNGC 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 404
Csp6I GTAC 3 cut(s) 252, 410, 495
CviAII CATG 1 cut(s) 458
CviJI RGCY 6 cut(s) 79, 85, 110, 127, 134, 290
CviKI_1 RGCY 6 cut(s) 79, 85, 110, 127, 134, 290
CviQI GTAC 3 cut(s) 252, 410, 495
DdeI CTNAG 3 cut(s) 240, 263, 320
EaeI YGGCCR 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 404
Eco57I CTGAAG 1 cut(s) 198
Eco88I CYCGRG 2 cut(s) 224, 247
EcoO109I RGGNCCY 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 192
FaeI CATG 1 cut(s) 461
FaiI YATR 4 cut(s) 17, 168, 459, 523
FaqI GGGAC 1 cut(s) 175
FatI CATG 1 cut(s) 457
Fnu4HI GCNGC 2 cut(s) 171, 375
FokI GGATG 3 cut(s) 139, 383, 482
Fsp4HI GCNGC 2 cut(s) 171, 375
FspBI CTAG 2 cut(s) 159, 378
GluI GCNGC 2 cut(s) 171, 375
GsuI CTGGAG 2 cut(s) 198, 594
HaeIII GGCC 2 cut(s) 110, 290
Hin1II CATG 1 cut(s) 461
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 4 cut(s) 188, 350, 432, 545
HphI GGTGA 2 cut(s) 284, 285
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 3 cut(s) 69, 131, 241
Hpy188III TCNNGA 4 cut(s) 138, 176, 215, 310
Hpy8I GTNNAC 1 cut(s) 463
HpyAV CCTTC 2 cut(s) 45, 575
HpyCH4III ACNGT 3 cut(s) 150, 440, 597
HpyCH4V TGCA 5 cut(s) 62, 357, 473, 533, 556
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 3 cut(s) 240, 263, 320
Hsp92II CATG 1 cut(s) 461
LmnI GCTCC 2 cut(s) 132, 178
LpnPI CCDG 9 cut(s) 179, 189, 206, 228, 295, 367, 420, 558, 582
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 2 cut(s) 361, 460
MaeI CTAG 2 cut(s) 159, 378
MbiI CCGCTC 1 cut(s) 173
MboII GAAGA 6 cut(s) 3, 12, 48, 81, 287, 359
MluCI AATT 3 cut(s) 30, 52, 489
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 92, 211
MseI TTAA 3 cut(s) 122, 315, 551
MslI CAYNNNNRTG 1 cut(s) 468
MspR9I CCNGG 1 cut(s) 194
Mva1269I GAATGC 1 cut(s) 394
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 461
NlaIV GGNNCC 2 cut(s) 405, 479
NmeAIII GCCGAG 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 224
PceI AGGCCT 1 cut(s) 290
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 3 cut(s) 350, 432, 545
PkrI GCNGC 2 cut(s) 172, 376
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 2 cut(s) 405, 479
PspPI GGNCC 1 cut(s) 404
PspPPI RGGWCCY 1 cut(s) 404
PspXI VCTCGAGB 1 cut(s) 224
RsaI GTAC 3 cut(s) 253, 411, 496
RsaNI GTAC 3 cut(s) 252, 410, 495
RseI CAYNNNNRTG 1 cut(s) 468
SaqAI TTAA 3 cut(s) 122, 315, 551
SatI GCNGC 2 cut(s) 171, 375
Sau96I GGNCC 1 cut(s) 404
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 194
SetI ASST 9 cut(s) 87, 129, 136, 214, 231, 326, 479, 567, 591
SfaNI GCATC 2 cut(s) 361, 460
Sfr274I CTCGAG 1 cut(s) 224
SinI GGWCC 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 3 cut(s) 30, 52, 489
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 1 cut(s) 171
SspMI CTAG 2 cut(s) 159, 378
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 3 cut(s) 150, 440, 597
TaqI TCGA 1 cut(s) 225
TasI AATT 3 cut(s) 30, 52, 489
TatI WGTACW 1 cut(s) 251
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 3 cut(s) 350, 432, 545
Tru1I TTAA 3 cut(s) 122, 315, 551
Tru9I TTAA 3 cut(s) 122, 315, 551
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 1 cut(s) 489
XhoI CTCGAG 1 cut(s) 224
XspI CTAG 2 cut(s) 159, 378
ZrmI AGTACT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.