Rh5AG356500

30S ribosomal protein S10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010010
Physical Location & Seq
Forward (+)
59150995 .. 59154301
3307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG356500.1

Sequence Viewer

Length: 594 bp
ATGGCGGTTTCTTCTATTTCCGCAACCCTAATTCCTTCTCTTCCCCTCTCCAATTCTTCTCCATTTCCTTCCTCTAAAGCCAAGCTCACTTCTTTCTCTCTGCCCGCTTCCCACAATGCCACAAAGTTTCCAGCTTTGAGACCCTCACTGTCGTCATCCACTAGGGTCTATGCTGCTCCTGAAGTCCTCGATAGCCCAGTTCCGCCTCCAGAAATCCTTCAAGGGCCCAATTCCGACGAGACTCCGAGCACTTCGTCACTCAGCATTGGTGGGGATGATAAGATAACACCGAAGCAGAAGATTAGGATTAAGCTTAGATCGTACTTTGTGCCTCTGATAGAGGATTCTTGCAAGCAGATAATGGATGCTGCAAGGAACACAAATGCAAAGACTATGGGCCCTGTGCCCTTACCAACCAAAAAGCGGATCTACTGTGTTCTGAAATCACCCCATGTCCACAAGGATGCAAGGTTCCATTTTGAGATCCGTACCCACCAGCGACTTATTGACATTCTTTACCCAACTGCCCAGACGATCGATTCCTTGATGCAACTTGACCTTCCTGCTGGAGTTGATGTTGAGGTCAAGCTTTGA

Protein Analysis

197

Amino Acids

21.48

Weight (kDa)

9.39

Isoelectric Point (pI)

67.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S10 PF00338 102 - 196 1.4e-33 Ribosomal protein S10p/S20e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13120 AT3G13120 AT3G13120
fragaria_vesca FvH4_3g29500
malus_domestica MD03G1187000.v1.1 MD11G1202000.v1.1
prunus_persica Prupe.4G239600_v2.0.a1
pyrus_communis pycom03g13930 pycom11g17450
rosa_chinensis RchiOBHm_Chr5g0054381
rosa_laevigata RLG00000034928
rosa_multiflora Rmu_sc0005705.1_g000022
rosa_roxburghii Rroxscaffold_1G00026490
rosa_rugosa Rorug05G0286000 Rorug05G0286000
rosa_samantha Rh5AG356500 Rh5BG368100 Rh5CG391400 Rh5DG382300
rosa_wichuraiana Rw5G033490 Rw5G033660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 5, 21, 105, 203, 424
AclWI GGATC 2 cut(s) 434, 478
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 2 cut(s) 323, 490
AfiI CCNNNNNNNGG 1 cut(s) 423
AgsI TTSAA 1 cut(s) 221
AloI GAACNNNNNNTCC 2 cut(s) 455, 487
AluBI AGCT 4 cut(s) 85, 134, 313, 589
AluI AGCT 4 cut(s) 85, 134, 313, 589
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 2 cut(s) 133, 233
AlwI GGATC 2 cut(s) 434, 478
AoxI GGCC 2 cut(s) 224, 397
ApaI GGGCCC 2 cut(s) 228, 401
ApeKI GCWGC 2 cut(s) 173, 368
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 4 cut(s) 224, 225, 397, 398
AsuHPI GGTGA 1 cut(s) 438
BaeGI GKGCMC 3 cut(s) 228, 401, 408
BanII GRGCYC 2 cut(s) 228, 401
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 2 cut(s) 160, 355
BcoDI GTCTC 2 cut(s) 133, 233
BfaI CTAG 1 cut(s) 162
BisI GCNGC 2 cut(s) 174, 369
BlsI GCNGC 2 cut(s) 175, 370
BmgT120I GGNCC 4 cut(s) 224, 225, 397, 398
BmiI GGNNCC 3 cut(s) 226, 399, 473
BmrI ACTGGG 1 cut(s) 191
BmsI GCATC 3 cut(s) 355, 454, 537
BmuI ACTGGG 1 cut(s) 191
BpmI CTGGAG 2 cut(s) 192, 588
Bsa29I ATCGAT 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 1 cut(s) 423
Bse1I ACTGG 1 cut(s) 197
BseCI ATCGAT 1 cut(s) 537
BseGI GGATG 4 cut(s) 155, 280, 370, 469
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 274
BseNI ACTGG 1 cut(s) 197
BseSI GKGCMC 3 cut(s) 228, 401, 408
BseXI GCAGC 2 cut(s) 160, 355
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 2 cut(s) 226, 399
BshVI ATCGAT 1 cut(s) 537
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 423
BsmAI GTCTC 2 cut(s) 133, 233
BsnI GGCC 2 cut(s) 226, 399
Bso31I GGTCTC 1 cut(s) 133
Bsp120I GGGCCC 2 cut(s) 224, 397
Bsp1286I GDGCHC 4 cut(s) 228, 251, 401, 408
Bsp143I GATC 4 cut(s) 317, 426, 483, 534
BspACI CCGC 5 cut(s) 5, 21, 105, 203, 424
BspANI GGCC 2 cut(s) 226, 399
BspCNI CTCAG 1 cut(s) 273
BspDI ATCGAT 1 cut(s) 537
BspLI GGNNCC 3 cut(s) 226, 399, 473
BspPI GGATC 2 cut(s) 434, 478
BspTNI GGTCTC 1 cut(s) 133
BsrI ACTGG 1 cut(s) 197
BssMI GATC 4 cut(s) 317, 426, 483, 534
Bst4CI ACNGT 2 cut(s) 150, 434
Bst6I CTCTTC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 105, 353
BstDEI CTNAG 2 cut(s) 260, 314
BstF5I GGATG 4 cut(s) 155, 280, 370, 469
BstKTI GATC 4 cut(s) 320, 429, 486, 537
BstMAI GTCTC 2 cut(s) 133, 233
BstMBI GATC 4 cut(s) 317, 426, 483, 534
BstMCI CGRYCG 1 cut(s) 537
BstSLI GKGCMC 3 cut(s) 228, 401, 408
BstV1I GCAGC 2 cut(s) 160, 355
BstX2I RGATCY 2 cut(s) 426, 483
BstYI RGATCY 2 cut(s) 426, 483
Bsu15I ATCGAT 1 cut(s) 537
BsuRI GGCC 2 cut(s) 226, 399
BsuTUI ATCGAT 1 cut(s) 537
BtsCI GGATG 4 cut(s) 155, 280, 370, 469
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 2 cut(s) 105, 353
Cfr13I GGNCC 4 cut(s) 224, 225, 397, 398
ClaI ATCGAT 1 cut(s) 537
Csp6I GTAC 2 cut(s) 322, 489
CviAII CATG 1 cut(s) 452
CviJI RGCY 8 cut(s) 80, 85, 134, 195, 226, 313, 399, 589
CviKI_1 RGCY 8 cut(s) 80, 85, 134, 195, 226, 313, 399, 589
CviQI GTAC 2 cut(s) 322, 489
DdeI CTNAG 2 cut(s) 260, 314
DpnI GATC 4 cut(s) 319, 428, 485, 536
DpnII GATC 4 cut(s) 317, 426, 483, 534
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 1 cut(s) 192
Eco24I GRGCYC 2 cut(s) 228, 401
Eco31I GGTCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 201
EcoO109I RGGNCCY 2 cut(s) 224, 398
EcoT38I GRGCYC 2 cut(s) 228, 401
FaeI CATG 1 cut(s) 455
FaiI YATR 3 cut(s) 171, 395, 453
FatI CATG 1 cut(s) 451
FauI CCCGC 1 cut(s) 112
Fnu4HI GCNGC 2 cut(s) 174, 369
FokI GGATG 4 cut(s) 142, 287, 377, 476
FriOI GRGCYC 2 cut(s) 228, 401
Fsp4HI GCNGC 2 cut(s) 174, 369
FspBI CTAG 1 cut(s) 162
GluI GCNGC 2 cut(s) 174, 369
GsuI CTGGAG 2 cut(s) 192, 588
HaeIII GGCC 2 cut(s) 226, 399
Hin1II CATG 1 cut(s) 455
HindIII AAGCTT 2 cut(s) 311, 587
HinfI GANTC 3 cut(s) 241, 344, 539
HphI GGTGA 1 cut(s) 438
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 4 cut(s) 235, 246, 336, 441
Hpy188III TCNNGA 2 cut(s) 179, 209
Hpy8I GTNNAC 1 cut(s) 457
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 4 cut(s) 45, 78, 227, 569
HpyCH4III ACNGT 2 cut(s) 150, 434
HpyCH4V TGCA 5 cut(s) 351, 371, 386, 467, 550
HpyF3I CTNAG 2 cut(s) 260, 314
Hsp92II CATG 1 cut(s) 455
Kzo9I GATC 4 cut(s) 317, 426, 483, 534
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 9 cut(s) 144, 192, 210, 222, 414, 509, 542, 552, 576
Lsp1109I GCAGC 2 cut(s) 160, 355
LweI GCATC 3 cut(s) 355, 454, 537
MaeI CTAG 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 255
MalI GATC 4 cut(s) 319, 428, 485, 536
MboI GATC 4 cut(s) 317, 426, 483, 534
MboII GAAGA 4 cut(s) 3, 32, 48, 310
MflI RGATCY 2 cut(s) 426, 483
MhlI GDGCHC 4 cut(s) 228, 251, 401, 408
MluCI AATT 3 cut(s) 30, 52, 229
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 8 cut(s) 56, 82, 154, 197, 216, 334, 342, 574
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 462
NdeII GATC 4 cut(s) 317, 426, 483, 534
NlaIII CATG 1 cut(s) 455
NlaIV GGNNCC 3 cut(s) 226, 399, 473
NmuCI GTSAC 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 344, 539
PkrI GCNGC 2 cut(s) 175, 370
Ple19I CGATCG 1 cut(s) 537
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 226, 399, 473
PspOMI GGGCCC 2 cut(s) 224, 397
PspPI GGNCC 4 cut(s) 224, 225, 397, 398
PsuI RGATCY 2 cut(s) 426, 483
PvuI CGATCG 1 cut(s) 537
RsaI GTAC 2 cut(s) 323, 490
RsaNI GTAC 2 cut(s) 322, 489
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 174, 369
Sau3AI GATC 4 cut(s) 317, 426, 483, 534
Sau96I GGNCC 4 cut(s) 224, 225, 397, 398
SchI GAGTC 1 cut(s) 235
SduI GDGCHC 4 cut(s) 228, 251, 401, 408
SetI ASST 7 cut(s) 87, 136, 315, 473, 561, 585, 591
SfaNI GCATC 3 cut(s) 355, 454, 537
SmiMI CAYNNNNRTG 1 cut(s) 462
Sse9I AATT 3 cut(s) 30, 52, 229
SsiI CCGC 5 cut(s) 5, 21, 105, 203, 424
SspMI CTAG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 150, 434
TaqI TCGA 2 cut(s) 189, 537
TasI AATT 3 cut(s) 30, 52, 229
TfiI GAWTC 2 cut(s) 344, 539
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 173, 368
Tsp45I GTSAC 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.