MD03G1193400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010819
Physical Location & Seq
Forward (+)
26586525 .. 26588921
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1193400.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGAAGGACTCGGGTCGAGATTGGGCAGGGCGTCCTCTAGGTACGGCCAAACAGCCACCGTCTTCAATGGCCCCGTCCGGAGGTGGAAGAAGAGGTGGGTCCACGTGTCCTCTTCATCCCCCTCCCTTACTTACCAAAACCCTCGCTCCCAATCCAACAACAACGGCAATAACTCTCGCCTCGTCCTCTGCCGCTGGACCGCAATCTCTCCCGCCAACTCCCCCGCCGAACCTGCCGCCGCTCCGGATGAGCCCCCTCGCCGGAAGTTCCGCTACACGCCCGTTTCTGTGGTAGAGGAACATAAAAAGGGAATTCAGAAAAAGGTTGGAGATGCTGAGAAAACTAGTGAGGTTGATGAATCAGTTACCCCGAGTGATGAGATGCATGTAAAACCAAACATTGGTCAAATTATGAAGGAACAAGATGAGGAGTCGGACGAGAACCAGTCTGGCTTGCAGGATTCAAACAGAAGCCATCTGGATTTAGATTTGGGCTTGAAAGGTCACAGTTCCAACCTTGATTTAATAGTTCAGGTGAAGAAGGGAAGCTCAGAGAGATTTTGGTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.18

Weight (kDa)

8.67

Isoelectric Point (pI)

71.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 241
AccB7I CCANNNNNTGG 1 cut(s) 401
AccBSI CCGCTC 1 cut(s) 242
AccIII TCCGGA 2 cut(s) 78, 244
AciI CCGC 7 cut(s) 193, 201, 213, 225, 237, 240, 271
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 312
AcvI CACGTG 1 cut(s) 106
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 3 cut(s) 81, 261, 401
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 3 cut(s) 67, 465, 499
AhlI ACTAGT 1 cut(s) 344
AluBI AGCT 1 cut(s) 549
AluI AGCT 1 cut(s) 549
Ama87I CYCGRG 2 cut(s) 11, 370
Aor13HI TCCGGA 2 cut(s) 78, 244
AoxI GGCC 2 cut(s) 46, 70
ApoI RAATTY 1 cut(s) 312
AspS9I GGNCC 3 cut(s) 71, 100, 198
AsuHPI GGTGA 1 cut(s) 547
AvaI CYCGRG 2 cut(s) 11, 370
AvaII GGWCC 2 cut(s) 100, 198
BanII GRGCYC 1 cut(s) 255
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BbrPI CACGTG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 55
BccI CCATC 1 cut(s) 483
BceAI ACGGC 2 cut(s) 61, 181
BcuI ACTAGT 1 cut(s) 344
BfaI CTAG 2 cut(s) 39, 345
BfuAI ACCTGC 1 cut(s) 241
BisI GCNGC 3 cut(s) 193, 237, 240
BlsI GCNGC 3 cut(s) 194, 238, 241
Bme18I GGWCC 2 cut(s) 100, 198
BmeT110I CYCGRG 2 cut(s) 11, 370
BmgT120I GGNCC 3 cut(s) 71, 100, 198
BmiI GGNNCC 2 cut(s) 73, 101
BmsI GCATC 2 cut(s) 322, 372
BoxI GACNNNNGTC 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 55
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 32
BsaWI WCCGGW 2 cut(s) 78, 244
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 261, 401
Bse1I ACTGG 1 cut(s) 445
BseAI TCCGGA 2 cut(s) 78, 244
BseGI GGATG 2 cut(s) 116, 253
BseLI CCNNNNNNNGG 3 cut(s) 81, 261, 401
BseMII CTCAG 2 cut(s) 327, 564
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 1 cut(s) 443
BshFI GGCC 2 cut(s) 48, 72
BsiHKCI CYCGRG 2 cut(s) 11, 370
BsiSI CCGG 3 cut(s) 79, 245, 262
BslI CCNNNNNNNGG 3 cut(s) 81, 261, 401
BsnI GGCC 2 cut(s) 48, 72
BsoBI CYCGRG 2 cut(s) 11, 370
Bsp1286I GDGCHC 1 cut(s) 255
Bsp13I TCCGGA 2 cut(s) 78, 244
BspACI CCGC 7 cut(s) 193, 201, 213, 225, 237, 240, 271
BspANI GGCC 2 cut(s) 48, 72
BspCNI CTCAG 2 cut(s) 328, 563
BspEI TCCGGA 2 cut(s) 78, 244
BspLI GGNNCC 2 cut(s) 73, 101
BspMI ACCTGC 1 cut(s) 241
BsrBI CCGCTC 1 cut(s) 242
BsrI ACTGG 1 cut(s) 445
BssNI GRCGYC 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 61, 509
Bst6I CTCTTC 2 cut(s) 86, 118
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 455
BstDEI CTNAG 2 cut(s) 336, 550
BstF5I GGATG 2 cut(s) 116, 253
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNSI RCATGY 1 cut(s) 389
BstPAI GACNNNNGTC 1 cut(s) 13
BstV2I GAAGAC 1 cut(s) 55
BsuRI GGCC 2 cut(s) 48, 72
BtsCI GGATG 2 cut(s) 116, 253
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 1 cut(s) 455
Cfr13I GGNCC 3 cut(s) 71, 100, 198
CseI GACGC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 43
CviAII CATG 1 cut(s) 386
CviJI RGCY 8 cut(s) 48, 56, 72, 253, 453, 474, 495, 549
CviKI_1 RGCY 8 cut(s) 48, 56, 72, 253, 453, 474, 495, 549
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 2 cut(s) 336, 550
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 86, 118
EarI CTCTTC 2 cut(s) 86, 118
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 100, 198
Eco72I CACGTG 1 cut(s) 106
Eco88I CYCGRG 2 cut(s) 11, 370
EcoRI GAATTC 1 cut(s) 312
EcoT22I ATGCAT 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 255
FaeI CATG 1 cut(s) 389
FaiI YATR 3 cut(s) 303, 387, 413
FatI CATG 1 cut(s) 385
FauI CCCGC 2 cut(s) 220, 232
Fnu4HI GCNGC 3 cut(s) 193, 237, 240
FokI GGATG 2 cut(s) 103, 260
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 3 cut(s) 193, 237, 240
FspBI CTAG 2 cut(s) 39, 345
GluI GCNGC 3 cut(s) 193, 237, 240
HaeIII GGCC 2 cut(s) 48, 72
HapII CCGG 3 cut(s) 79, 245, 262
HgaI GACGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 1 cut(s) 389
HinfI GANTC 4 cut(s) 9, 359, 431, 461
HpaII CCGG 3 cut(s) 79, 245, 262
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 3 cut(s) 318, 436, 553
Hpy188III TCNNGA 4 cut(s) 18, 79, 245, 479
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 409, 535
HpyCH4III ACNGT 2 cut(s) 61, 509
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 2 cut(s) 385, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 2 cut(s) 336, 550
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 1 cut(s) 389
Kpn2I TCCGGA 2 cut(s) 78, 244
LmnI GCTCC 2 cut(s) 152, 247
LweI GCATC 2 cut(s) 322, 372
MaeI CTAG 2 cut(s) 39, 345
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 364, 503
MbiI CCGCTC 1 cut(s) 242
MboII GAAGA 5 cut(s) 55, 100, 103, 105, 550
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 2 cut(s) 312, 408
MlyI GAGTC 2 cut(s) 3, 440
MmeI TCCRAC 5 cut(s) 180, 307, 414, 537, 546
Mph1103I ATGCAT 1 cut(s) 387
MroI TCCGGA 2 cut(s) 78, 244
MseI TTAA 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 3 cut(s) 79, 245, 262
MwoI GCNNNNNNNGC 1 cut(s) 233
NlaIII CATG 1 cut(s) 389
NlaIV GGNNCC 2 cut(s) 73, 101
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 387
NspI RCATGY 1 cut(s) 389
PfeI GAWTC 2 cut(s) 359, 461
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 3 cut(s) 194, 238, 241
PleI GAGTC 2 cut(s) 3, 439
PmaCI CACGTG 1 cut(s) 106
PmlI CACGTG 1 cut(s) 106
PpsI GAGTC 2 cut(s) 3, 439
Ppu21I YACGTR 1 cut(s) 106
PshAI GACNNNNGTC 1 cut(s) 13
PspCI CACGTG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 73, 101
PspPI GGNCC 3 cut(s) 71, 100, 198
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SaqAI TTAA 1 cut(s) 524
SatI GCNGC 3 cut(s) 193, 237, 240
Sau96I GGNCC 3 cut(s) 71, 100, 198
SchI GAGTC 2 cut(s) 3, 440
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 2 cut(s) 322, 372
SinI GGWCC 2 cut(s) 100, 198
SpeI ACTAGT 1 cut(s) 344
Sse9I AATT 2 cut(s) 312, 408
SsiI CCGC 7 cut(s) 193, 201, 213, 225, 237, 240, 271
SspMI CTAG 2 cut(s) 39, 345
TaaI ACNGT 2 cut(s) 61, 509
TaiI ACGT 1 cut(s) 108
TaqI TCGA 1 cut(s) 17
TasI AATT 2 cut(s) 312, 408
TauI GCSGC 3 cut(s) 195, 239, 242
TfiI GAWTC 2 cut(s) 359, 461
Tru1I TTAA 1 cut(s) 524
Tru9I TTAA 1 cut(s) 524
TseFI GTSAC 1 cut(s) 503
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 3 cut(s) 105, 372, 428
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 100, 198
XapI RAATTY 1 cut(s) 312
XceI RCATGY 1 cut(s) 389
XspI CTAG 2 cut(s) 39, 345
Zsp2I ATGCAT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.