MD11G1209600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010819
Physical Location & Seq
Forward (+)
30651174 .. 30653615
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1209600.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGAAGCACTCGGGTCGAGATTGGGCAGGGCGTCCTCCAGGTACGGCCAAACAGCCACCGTCTTCAACGGCCCCGTCCGCAGGTGGAAGAAGAGGTGGGTCCACGTGTCCTCTTCATCCCCCTCCCTTACTTACCAAAACCCTCACTCCCAATCCAACAACAGCAGCTCTCGCCTCCTCCTCTGCCGCTGGACCCCAATCTCTTCCGCTACCTCCCCCGACGAACCCGCCGCGGCTTCGGATGAGCCCCCTCGCCGGAAGTTCCGCTACACGCCCGTTTCTGTGCTAGAGGAACAGAAAAAGCCAATTCAGAAAAAGGTTGGAGATGAGGAGAAAACTAGTGAGACTGATGAATCGGTTAGCCCAAGTGATAAGGTGCATCGAAAGCCAAATATTAATCAGATTATGAAGGAACAAGCTGAGGAGTTGGACGAGGACGAATCAGGAGTGCAGGATTCAAACAGAAGCCATCTGGATTTAGATTTGGGCTTGAAAGATGGTCACAATGTCAACCTTGATTCAATGATTCAGATGAAGAAAGCAAGTTCAGGTAGATTTTGGTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

21.28

Weight (kDa)

7.86

Isoelectric Point (pI)

69.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 72
AccII CGCG 1 cut(s) 233
AciI CCGC 7 cut(s) 79, 187, 207, 228, 231, 233, 265
AcoI YGGCCR 1 cut(s) 46
AcvI CACGTG 1 cut(s) 106
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 2 cut(s) 81, 255
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 4 cut(s) 67, 459, 493, 522
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 1 cut(s) 38
AluBI AGCT 2 cut(s) 168, 419
AluI AGCT 2 cut(s) 168, 419
Alw26I GTCTC 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 2 cut(s) 46, 70
ApeKI GCWGC 1 cut(s) 165
AseI ATTAAT 1 cut(s) 396
AspS9I GGNCC 3 cut(s) 71, 100, 192
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 2 cut(s) 100, 192
BanII GRGCYC 1 cut(s) 249
BarI GAAGNNNNNNTAC 2 cut(s) 251, 283
BbrPI CACGTG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 55
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 177
BccI CCATC 2 cut(s) 477, 491
BceAI ACGGC 2 cut(s) 61, 85
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 338
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 2 cut(s) 287, 339
BfuAI ACCTGC 1 cut(s) 72
BisI GCNGC 4 cut(s) 166, 187, 231, 234
BlsI GCNGC 4 cut(s) 167, 188, 232, 235
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 2 cut(s) 100, 192
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 3 cut(s) 71, 100, 192
BmiI GGNNCC 3 cut(s) 73, 101, 194
BmrFI CCNGG 1 cut(s) 40
BmsI GCATC 1 cut(s) 388
BpiI GAAGAC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 420
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 255
BseBI CCWGG 1 cut(s) 40
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 2 cut(s) 116, 247
BseLI CCNNNNNNNGG 2 cut(s) 81, 255
BseMII CTCAG 1 cut(s) 411
BseRI GAGGAG 4 cut(s) 167, 170, 344, 437
BseXI GCAGC 1 cut(s) 177
BsgI GTGCAG 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 233
BshFI GGCC 2 cut(s) 48, 72
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 256
BslI CCNNNNNNNGG 2 cut(s) 81, 255
BsmAI GTCTC 1 cut(s) 338
BsnI GGCC 2 cut(s) 48, 72
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 249
BspACI CCGC 7 cut(s) 79, 187, 207, 228, 231, 233, 265
BspANI GGCC 2 cut(s) 48, 72
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 3 cut(s) 73, 101, 194
BspMI ACCTGC 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 231
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 1 cut(s) 40
Bst4CI ACNGT 1 cut(s) 61
Bst6I CTCTTC 3 cut(s) 86, 118, 208
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 420
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 116, 247
BstFNI CGCG 1 cut(s) 233
BstMAI GTCTC 1 cut(s) 338
BstMWI GCNNNNNNNGC 3 cut(s) 78, 171, 385
BstNI CCWGG 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 38
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 1 cut(s) 177
BstV2I GAAGAC 1 cut(s) 55
BsuRI GGCC 2 cut(s) 48, 72
BtgI CCRYGG 1 cut(s) 231
BtsCI GGATG 2 cut(s) 116, 247
BveI ACCTGC 1 cut(s) 72
Cfr13I GGNCC 3 cut(s) 71, 100, 192
Cfr42I CCGCGG 1 cut(s) 234
CseI GACGC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 43
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 1 cut(s) 420
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 3 cut(s) 86, 118, 208
EarI CTCTTC 3 cut(s) 86, 118, 208
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 100, 192
Eco72I CACGTG 1 cut(s) 106
Eco88I CYCGRG 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 249
FaiI YATR 1 cut(s) 407
FauI CCCGC 1 cut(s) 235
Fnu4HI GCNGC 4 cut(s) 166, 187, 231, 234
FokI GGATG 2 cut(s) 103, 254
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 166, 187, 231, 234
FspBI CTAG 2 cut(s) 287, 339
GluI GCNGC 4 cut(s) 166, 187, 231, 234
GsuI CTGGAG 1 cut(s) 22
HaeIII GGCC 2 cut(s) 48, 72
HapII CCGG 1 cut(s) 256
HgaI GACGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 5 cut(s) 353, 440, 455, 518, 526
HpaII CCGG 1 cut(s) 256
Hpy166II GTNNAC 2 cut(s) 103, 511
Hpy188I TCNGA 4 cut(s) 241, 312, 402, 531
Hpy188III TCNNGA 3 cut(s) 18, 444, 473
Hpy8I GTNNAC 2 cut(s) 103, 511
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 2 cut(s) 379, 451
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 171, 385
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 32
KspI CCGCGG 1 cut(s) 234
Lsp1109I GCAGC 1 cut(s) 177
LweI GCATC 1 cut(s) 388
MaeI CTAG 2 cut(s) 287, 339
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 1 cut(s) 500
MboII GAAGA 6 cut(s) 55, 100, 103, 105, 195, 547
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 1 cut(s) 306
MmeI TCCRAC 4 cut(s) 180, 301, 408, 543
MseI TTAA 1 cut(s) 396
MspA1I CMGCKG 2 cut(s) 189, 233
MspI CCGG 1 cut(s) 256
MspR9I CCNGG 1 cut(s) 40
MvaI CCWGG 1 cut(s) 40
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 3 cut(s) 78, 171, 385
NlaIV GGNNCC 3 cut(s) 73, 101, 194
NmuCI GTSAC 1 cut(s) 500
PaqCI CACCTGC 1 cut(s) 72
PfeI GAWTC 5 cut(s) 353, 440, 455, 518, 526
PkrI GCNGC 4 cut(s) 167, 188, 232, 235
PmaCI CACGTG 1 cut(s) 106
PmlI CACGTG 1 cut(s) 106
Ppu21I YACGTR 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 38
PspCI CACGTG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 38
PspN4I GGNNCC 3 cut(s) 73, 101, 194
PspPI GGNCC 3 cut(s) 71, 100, 192
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SacII CCGCGG 1 cut(s) 234
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 4 cut(s) 166, 187, 231, 234
Sau96I GGNCC 3 cut(s) 71, 100, 192
ScrFI CCNGG 1 cut(s) 40
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 1 cut(s) 388
Sfr303I CCGCGG 1 cut(s) 234
SgrBI CCGCGG 1 cut(s) 234
SinI GGWCC 2 cut(s) 100, 192
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 1 cut(s) 306
SsiI CCGC 7 cut(s) 79, 187, 207, 228, 231, 233, 265
SspI AATATT 1 cut(s) 394
SspMI CTAG 2 cut(s) 287, 339
StyD4I CCNGG 1 cut(s) 38
TaaI ACNGT 1 cut(s) 61
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 17, 382
TasI AATT 1 cut(s) 306
TauI GCSGC 3 cut(s) 189, 233, 236
TfiI GAWTC 5 cut(s) 353, 440, 455, 518, 526
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 1 cut(s) 165
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 4 cut(s) 105, 366, 422, 548
VpaK11BI GGWCC 2 cut(s) 100, 192
VspI ATTAAT 1 cut(s) 396
XspI CTAG 2 cut(s) 287, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.