Prupe.4G223100_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0010819
Physical Location & Seq
Forward (+)
14007947 .. 14010656
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G223100.1

Sequence Viewer

Length: 597 bp
ATGGAGGGAGTGGGGTCCAGATTGGGCCGGGCCTCCTCGAGGTACGGCCAAACCGCCACCGTCTTCAGCGGCCCAGTTCGCAGGTGGAAGAAGAAGTGGGTCCACGTGCCCTCATCATCCTCCGTTACTTACCAGAACCCTCACTCCCAATCCAACGGCAACAACAACAGCTCCCGCCTCCTCCTCCGCCGCTGGACCCCAATCTCTCCGGCCACCACCGCCGCAGAACCCCTCTCCGGCTCCGGCGCCTCGACGGAGGAGCCGCCGCGCCGGAAGTTCCGCTACACGCCCGTTGCTGTGCTAGAGGAACAGAAGAAGACAGCCAAGGGAAAGGTTGGAGATGTGGTGAAAGCTAGTGAGGGAGGCCAATCTATAGCCACGAGTACTAAGATGCATGAAATTTCAGACATTGATCAAGATATGGAGGAACAAGCTGAGGAATTGGATGAGACCCATTCTGGCTTGCAGGATTCAAATGGAAGCCATCTGGATTTAGATTTGGGTTTGAAAGGTCACAGTGCCAACCGTGATTCCATTAGCCAGATTGAAGATGCTGAGATGAAAAAAGCAAGCCCGGGTAGATTTTGGTTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

21.58

Weight (kDa)

7.99

Isoelectric Point (pI)

68.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 72
AbsI CCTCGAGG 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 245
AccII CGCG 1 cut(s) 268
AcoI YGGCCR 2 cut(s) 46, 210
AcsI RAATTY 1 cut(s) 399
AcuI CTGAAG 1 cut(s) 49
AcvI CACGTG 1 cut(s) 106
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 2 cut(s) 44, 385
AfiI CCNNNNNNNGG 2 cut(s) 39, 236
AgsI TTSAA 3 cut(s) 474, 508, 548
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 3 cut(s) 171, 353, 434
AluI AGCT 3 cut(s) 171, 353, 434
Alw26I GTCTC 1 cut(s) 443
Ama87I CYCGRG 2 cut(s) 37, 574
AoxI GGCC 6 cut(s) 25, 30, 46, 70, 210, 364
ApoI RAATTY 1 cut(s) 399
AspLEI GCGC 2 cut(s) 248, 270
AspS9I GGNCC 6 cut(s) 15, 25, 30, 71, 100, 195
AsuC2I CCSGG 3 cut(s) 29, 575, 576
AsuHPI GGTGA 1 cut(s) 358
AvaI CYCGRG 2 cut(s) 37, 574
AvaII GGWCC 3 cut(s) 15, 100, 195
BaeGI GKGCMC 1 cut(s) 111
BanI GGYRCC 1 cut(s) 245
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BauI CACGAG 1 cut(s) 379
BbrPI CACGTG 1 cut(s) 106
BbsI GAAGAC 2 cut(s) 55, 323
BbvCI CCTCAGC 1 cut(s) 435
BccI CCATC 1 cut(s) 492
BceAI ACGGC 2 cut(s) 61, 172
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 3 cut(s) 29, 575, 576
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 302, 354
BfmI CTRYAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 72
BisI GCNGC 5 cut(s) 70, 190, 222, 263, 266
BlsI GCNGC 5 cut(s) 71, 191, 223, 264, 267
BmcAI AGTACT 1 cut(s) 385
Bme1390I CCNGG 3 cut(s) 29, 575, 576
Bme18I GGWCC 3 cut(s) 15, 100, 195
BmeT110I CYCGRG 2 cut(s) 37, 574
BmgT120I GGNCC 6 cut(s) 15, 25, 30, 71, 100, 195
BmiI GGNNCC 6 cut(s) 16, 101, 197, 241, 247, 261
BmrFI CCNGG 3 cut(s) 29, 575, 576
BmrI ACTGGG 1 cut(s) 68
BmsI GCATC 2 cut(s) 381, 541
BmuI ACTGGG 1 cut(s) 68
BpiI GAAGAC 2 cut(s) 55, 323
Bpu10I CCTNAGC 1 cut(s) 435
BpuMI CCSGG 3 cut(s) 29, 575, 576
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 324, 574
BsaXI ACNNNNNCTCC 6 cut(s) 128, 155, 158, 185, 330, 360
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 236
Bse1I ACTGG 1 cut(s) 74
BseDI CCNNGG 2 cut(s) 324, 574
BseGI GGATG 2 cut(s) 116, 451
BseLI CCNNNNNNNGG 2 cut(s) 39, 236
BseMII CTCAG 2 cut(s) 426, 546
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 4 cut(s) 25, 170, 173, 272
BseSI GKGCMC 1 cut(s) 111
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 6 cut(s) 27, 32, 48, 72, 212, 366
BshNI GGYRCC 1 cut(s) 245
BsiHKCI CYCGRG 2 cut(s) 37, 574
BsiSI CCGG 6 cut(s) 28, 209, 237, 243, 271, 575
BslI CCNNNNNNNGG 2 cut(s) 39, 236
BsmAI GTCTC 1 cut(s) 443
BsnI GGCC 6 cut(s) 27, 32, 48, 72, 212, 366
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 2 cut(s) 37, 574
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 412
BspANI GGCC 6 cut(s) 27, 32, 48, 72, 212, 366
BspCNI CTCAG 2 cut(s) 427, 547
BspFNI CGCG 1 cut(s) 268
BspLI GGNNCC 6 cut(s) 16, 101, 197, 241, 247, 261
BspMI ACCTGC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 443
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 324, 574
BssMI GATC 1 cut(s) 412
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 379
Bst4CI ACNGT 3 cut(s) 61, 518, 527
BstACI GRCGYC 1 cut(s) 246
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 464, 571
BstDEI CTNAG 3 cut(s) 387, 435, 555
BstENI CCTNNNNNAGG 1 cut(s) 37
BstF5I GGATG 2 cut(s) 116, 451
BstFNI CGCG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 2 cut(s) 248, 270
BstKTI GATC 1 cut(s) 415
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 1 cut(s) 412
BstMWI GCNNNNNNNGC 2 cut(s) 78, 218
BstSCI CCNGG 3 cut(s) 27, 573, 574
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 111
BstUI CGCG 1 cut(s) 268
BstV2I GAAGAC 2 cut(s) 55, 323
BsuRI GGCC 6 cut(s) 27, 32, 48, 72, 212, 366
BtsCI GGATG 2 cut(s) 116, 451
BtsIMutI CAGTG 1 cut(s) 523
BveI ACCTGC 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 464, 571
CfoI GCGC 2 cut(s) 248, 270
Cfr13I GGNCC 6 cut(s) 15, 25, 30, 71, 100, 195
Cfr9I CCCGGG 1 cut(s) 574
Csp6I GTAC 2 cut(s) 43, 384
CviAII CATG 1 cut(s) 395
CviQI GTAC 2 cut(s) 43, 384
DdeI CTNAG 3 cut(s) 387, 435, 555
DinI GGCGCC 1 cut(s) 247
DpnI GATC 1 cut(s) 414
DpnII GATC 1 cut(s) 412
EaeI YGGCCR 2 cut(s) 46, 210
EciI GGCGGA 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 3 cut(s) 15, 100, 195
Eco57I CTGAAG 1 cut(s) 49
Eco72I CACGTG 1 cut(s) 106
Eco88I CYCGRG 2 cut(s) 37, 574
EcoNI CCTNNNNNAGG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 324
EcoT22I ATGCAT 1 cut(s) 396
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 1 cut(s) 398
FaiI YATR 3 cut(s) 374, 396, 422
FatI CATG 1 cut(s) 394
FauI CCCGC 1 cut(s) 182
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 5 cut(s) 70, 190, 222, 263, 266
FokI GGATG 2 cut(s) 103, 458
Fsp4HI GCNGC 5 cut(s) 70, 190, 222, 263, 266
FspBI CTAG 2 cut(s) 302, 354
GlaI GCGC 2 cut(s) 247, 269
GluI GCNGC 5 cut(s) 70, 190, 222, 263, 266
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 6 cut(s) 27, 32, 48, 72, 212, 366
HapII CCGG 6 cut(s) 28, 209, 237, 243, 271, 575
HhaI GCGC 2 cut(s) 248, 270
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 1 cut(s) 398
Hin6I GCGC 2 cut(s) 246, 268
HinP1I GCGC 2 cut(s) 246, 268
HinfI GANTC 2 cut(s) 470, 530
HpaII CCGG 6 cut(s) 28, 209, 237, 243, 271, 575
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 1 cut(s) 406
Hpy188III TCNNGA 3 cut(s) 18, 416, 488
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 1 cut(s) 256
HpyCH4III ACNGT 3 cut(s) 61, 518, 527
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 2 cut(s) 394, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 218
HpyF3I CTNAG 3 cut(s) 387, 435, 555
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 1 cut(s) 398
HspAI GCGC 2 cut(s) 246, 268
KasI GGCGCC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 1 cut(s) 412
LmnI GCTCC 3 cut(s) 176, 245, 259
LweI GCATC 2 cut(s) 381, 541
MaeI CTAG 2 cut(s) 302, 354
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 124, 512
MalI GATC 1 cut(s) 414
MboI GATC 1 cut(s) 412
MboII GAAGA 6 cut(s) 55, 100, 103, 325, 328, 560
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 2 cut(s) 399, 440
Mly113I GGCGCC 1 cut(s) 246
MmeI TCCRAC 2 cut(s) 177, 316
Mph1103I ATGCAT 1 cut(s) 396
MspA1I CMGCKG 2 cut(s) 69, 192
MspI CCGG 6 cut(s) 28, 209, 237, 243, 271, 575
MspR9I CCNGG 3 cut(s) 29, 575, 576
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 2 cut(s) 78, 218
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 3 cut(s) 29, 575, 576
NdeII GATC 1 cut(s) 412
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 6 cut(s) 16, 101, 197, 241, 247, 261
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 396
PaeR7I CTCGAG 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 72
PfeI GAWTC 2 cut(s) 470, 530
PkrI GCNGC 5 cut(s) 71, 191, 223, 264, 267
PluTI GGCGCC 1 cut(s) 249
PmaCI CACGTG 1 cut(s) 106
PmlI CACGTG 1 cut(s) 106
Ppu21I YACGTR 1 cut(s) 106
PspCI CACGTG 1 cut(s) 106
PspN4I GGNNCC 6 cut(s) 16, 101, 197, 241, 247, 261
PspPI GGNCC 6 cut(s) 15, 25, 30, 71, 100, 195
PspXI VCTCGAGB 1 cut(s) 37
RsaI GTAC 2 cut(s) 44, 385
RsaNI GTAC 2 cut(s) 43, 384
SatI GCNGC 5 cut(s) 70, 190, 222, 263, 266
Sau3AI GATC 1 cut(s) 412
Sau96I GGNCC 6 cut(s) 15, 25, 30, 71, 100, 195
ScaI AGTACT 1 cut(s) 385
ScrFI CCNGG 3 cut(s) 29, 575, 576
SduI GDGCHC 1 cut(s) 111
SetI ASST 8 cut(s) 44, 86, 108, 173, 336, 355, 436, 514
SfaNI GCATC 2 cut(s) 381, 541
SfcI CTRYAG 1 cut(s) 372
SfoI GGCGCC 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 37
SinI GGWCC 3 cut(s) 15, 100, 195
SlaI CTCGAG 1 cut(s) 37
SmaI CCCGGG 1 cut(s) 576
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 2 cut(s) 399, 440
SspDI GGCGCC 1 cut(s) 245
SspMI CTAG 2 cut(s) 302, 354
StyD4I CCNGG 3 cut(s) 27, 573, 574
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 3 cut(s) 61, 518, 527
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 38, 251
TasI AATT 2 cut(s) 399, 440
TatI WGTACW 1 cut(s) 383
TauI GCSGC 5 cut(s) 72, 192, 224, 265, 268
TfiI GAWTC 2 cut(s) 470, 530
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 512
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 2 cut(s) 411, 575
TspGWI ACGGA 2 cut(s) 112, 269
TspMI CCCGGG 1 cut(s) 574
TspRI CASTG 1 cut(s) 523
VpaK11BI GGWCC 3 cut(s) 15, 100, 195
XagI CCTNNNNNAGG 1 cut(s) 37
XapI RAATTY 1 cut(s) 399
XcmI CCANNNNNNNNNTGG 1 cut(s) 81
XhoI CTCGAG 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 574
XspI CTAG 2 cut(s) 302, 354
ZrmI AGTACT 1 cut(s) 385
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.