Rh5BG283300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010819
Physical Location & Seq
Reverse (-)
36017067 .. 36019380
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG283300.1

Sequence Viewer

Length: 600 bp
ATGGAGGGAGCCGGGTCCAGACTGAGCCGAGCGTCCTCCAGGTACGGCCCAACAACCACCGTCTTCAGCGGCCCCGTCAGGAGGTGGAAGAAGAAGTGGGTCCACGTGCCGCCGTCACCGTCCTCCTTCAGCTCTCGCTCCCAATCCAACGCCCGAAACAACGACAACAGCTCTCGCCTCCTCCTTTGCCGCTGGACCCCAATTTCTTCTGCACCTAACTCCGCAGCCGCCGAGCCCGGCTCCGGCGACGAGCGGCCCCGCCGGAAGTTCCGCTACACGCCTGTTGTTTTGCTAGAGGAGCAGAGGAAGGCGGTTGAGAAAAGGATTGAGGACATGGAGGATGCGGAGAACGCTAGTGAGGGAGACCAATCTACTGTGCTGAGTGATAAGATGTATGGGAAGTCAAACGCTGATCAATTCAAGGAGCAAGAAGCTGAGGTAAGACCTTTAAACTCCTACTCAACTATCATTTGCATTTGTGCTTTTTGTTTGTGGTGTAGACTCTGCATTCCTCTGTGTTTAGGAACTGCTGTGGCCCCTCCCTGTCCAATAGCATCTTATAGGGTTATTGGTCACAGCTATTTAATTCATTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

22.13

Weight (kDa)

9.08

Isoelectric Point (pI)

63.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 253
AccI GTMKAC 1 cut(s) 499
AcuI CTGAAG 2 cut(s) 49, 112
AcvI CACGTG 1 cut(s) 106
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 2 cut(s) 81, 242
AgsI TTSAA 1 cut(s) 421
AjnI CCWGG 1 cut(s) 38
AluBI AGCT 4 cut(s) 132, 171, 434, 579
AluI AGCT 4 cut(s) 132, 171, 434, 579
Alw26I GTCTC 1 cut(s) 357
AoxI GGCC 4 cut(s) 46, 70, 254, 534
ApeKI GCWGC 1 cut(s) 224
AspS9I GGNCC 7 cut(s) 15, 47, 71, 100, 195, 255, 535
AsuC2I CCSGG 2 cut(s) 13, 237
AsuHPI GGTGA 1 cut(s) 108
AvaII GGWCC 3 cut(s) 15, 100, 195
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BbrPI CACGTG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 55
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 1 cut(s) 236
BceAI ACGGC 2 cut(s) 61, 97
BciT130I CCWGG 1 cut(s) 40
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 2 cut(s) 13, 237
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 2 cut(s) 293, 354
BisI GCNGC 6 cut(s) 70, 110, 190, 225, 228, 254
BlsI GCNGC 6 cut(s) 71, 111, 191, 226, 229, 255
Bme1390I CCNGG 3 cut(s) 13, 40, 237
Bme18I GGWCC 3 cut(s) 15, 100, 195
BmgT120I GGNCC 7 cut(s) 15, 47, 71, 100, 195, 255, 535
BmiI GGNNCC 8 cut(s) 10, 16, 73, 101, 197, 241, 257, 537
BmrFI CCNGG 3 cut(s) 13, 40, 237
BmsI GCATC 2 cut(s) 331, 563
BpiI GAAGAC 1 cut(s) 55
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpmI CTGGAG 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 435
BpuMI CCSGG 2 cut(s) 13, 237
BsaAI YACGTR 1 cut(s) 106
BsaI GGTCTC 1 cut(s) 357
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 242
BseBI CCWGG 1 cut(s) 40
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 2 cut(s) 81, 242
BseMII CTCAG 3 cut(s) 14, 371, 426
BseRI GAGGAG 2 cut(s) 170, 311
BseXI GCAGC 1 cut(s) 236
BsgI GTGCAG 1 cut(s) 195
BshFI GGCC 4 cut(s) 48, 72, 256, 536
BsiSI CCGG 4 cut(s) 12, 237, 243, 262
BslI CCNNNNNNNGG 2 cut(s) 81, 242
BsmAI GTCTC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 507
BsnI GGCC 4 cut(s) 48, 72, 256, 536
Bso31I GGTCTC 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 1 cut(s) 412
BspANI GGCC 4 cut(s) 48, 72, 256, 536
BspCNI CTCAG 3 cut(s) 15, 372, 427
BspLI GGNNCC 8 cut(s) 10, 16, 73, 101, 197, 241, 257, 537
BspTNI GGTCTC 1 cut(s) 357
BsrBI CCGCTC 1 cut(s) 253
BssMI GATC 1 cut(s) 412
Bst2UI CCWGG 1 cut(s) 40
Bst4CI ACNGT 3 cut(s) 61, 120, 376
BstBAI YACGTR 1 cut(s) 106
BstDEI CTNAG 3 cut(s) 23, 380, 435
BstF5I GGATG 1 cut(s) 346
BstKTI GATC 1 cut(s) 415
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 1 cut(s) 412
BstMWI GCNNNNNNNGC 2 cut(s) 298, 350
BstNI CCWGG 1 cut(s) 40
BstSCI CCNGG 3 cut(s) 11, 38, 235
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 55
BsuRI GGCC 4 cut(s) 48, 72, 256, 536
BtsCI GGATG 1 cut(s) 346
Cfr13I GGNCC 7 cut(s) 15, 47, 71, 100, 195, 255, 535
CseI GACGC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 43
CviAII CATG 1 cut(s) 334
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 3 cut(s) 23, 380, 435
DpnI GATC 1 cut(s) 414
DpnII GATC 1 cut(s) 412
DraI TTTAAA 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 357
Eco47I GGWCC 3 cut(s) 15, 100, 195
Eco57I CTGAAG 2 cut(s) 49, 112
Eco72I CACGTG 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 1 cut(s) 337
FaiI YATR 3 cut(s) 335, 396, 561
FatI CATG 1 cut(s) 333
FauI CCCGC 1 cut(s) 266
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 6 cut(s) 70, 110, 190, 225, 228, 254
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 6 cut(s) 70, 110, 190, 225, 228, 254
FspBI CTAG 2 cut(s) 293, 354
GluI GCNGC 6 cut(s) 70, 110, 190, 225, 228, 254
GsuI CTGGAG 1 cut(s) 22
HaeIII GGCC 4 cut(s) 48, 72, 256, 536
HapII CCGG 4 cut(s) 12, 237, 243, 262
HgaI GACGC 1 cut(s) 21
Hin1II CATG 1 cut(s) 337
HinfI GANTC 1 cut(s) 501
HpaII CCGG 4 cut(s) 12, 237, 243, 262
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 2 cut(s) 103, 500
Hpy188III TCNNGA 2 cut(s) 18, 79
Hpy8I GTNNAC 2 cut(s) 103, 500
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 2 cut(s) 136, 301
HpyCH4III ACNGT 3 cut(s) 61, 120, 376
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 3 cut(s) 212, 474, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 350
HpyF3I CTNAG 3 cut(s) 23, 380, 435
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 1 cut(s) 412
LmnI GCTCC 5 cut(s) 8, 143, 245, 298, 424
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 2 cut(s) 331, 563
MaeI CTAG 2 cut(s) 293, 354
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 114, 572
MalI GATC 1 cut(s) 414
MbiI CCGCTC 1 cut(s) 253
MboI GATC 1 cut(s) 412
MboII GAAGA 4 cut(s) 55, 100, 103, 198
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 3 cut(s) 201, 416, 585
MlyI GAGTC 1 cut(s) 495
MmeI TCCRAC 1 cut(s) 171
MseI TTAA 2 cut(s) 449, 584
MspA1I CMGCKG 2 cut(s) 69, 192
MspI CCGG 4 cut(s) 12, 237, 243, 262
MspR9I CCNGG 3 cut(s) 13, 40, 237
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 1 cut(s) 40
MwoI GCNNNNNNNGC 2 cut(s) 298, 350
NciI CCSGG 2 cut(s) 13, 237
NdeII GATC 1 cut(s) 412
NlaIII CATG 1 cut(s) 337
NlaIV GGNNCC 8 cut(s) 10, 16, 73, 101, 197, 241, 257, 537
NmeAIII GCCGAG 2 cut(s) 53, 256
NmuCI GTSAC 2 cut(s) 114, 572
PctI GAATGC 1 cut(s) 507
PkrI GCNGC 6 cut(s) 71, 111, 191, 226, 229, 255
PleI GAGTC 1 cut(s) 495
PmaCI CACGTG 1 cut(s) 106
PmlI CACGTG 1 cut(s) 106
PpsI GAGTC 1 cut(s) 495
Ppu21I YACGTR 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 38
PspCI CACGTG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 38
PspN4I GGNNCC 8 cut(s) 10, 16, 73, 101, 197, 241, 257, 537
PspPI GGNCC 7 cut(s) 15, 47, 71, 100, 195, 255, 535
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SaqAI TTAA 2 cut(s) 449, 584
SatI GCNGC 6 cut(s) 70, 110, 190, 225, 228, 254
Sau3AI GATC 1 cut(s) 412
Sau96I GGNCC 7 cut(s) 15, 47, 71, 100, 195, 255, 535
SchI GAGTC 1 cut(s) 495
ScrFI CCNGG 3 cut(s) 13, 40, 237
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 2 cut(s) 331, 563
SinI GGWCC 3 cut(s) 15, 100, 195
Sse9I AATT 3 cut(s) 201, 416, 585
SspMI CTAG 2 cut(s) 293, 354
StyD4I CCNGG 3 cut(s) 11, 38, 235
TaaI ACNGT 3 cut(s) 61, 120, 376
TaiI ACGT 1 cut(s) 108
TasI AATT 3 cut(s) 201, 416, 585
TauI GCSGC 5 cut(s) 72, 112, 192, 230, 256
Tru1I TTAA 2 cut(s) 449, 584
Tru9I TTAA 2 cut(s) 449, 584
TseFI GTSAC 2 cut(s) 114, 572
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 114, 572
TspDTI ATGAA 1 cut(s) 578
VpaK11BI GGWCC 3 cut(s) 15, 100, 195
XmiI GTMKAC 1 cut(s) 499
XspI CTAG 2 cut(s) 293, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.