MD03G1200300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009377
Physical Location & Seq
Forward (+)
27399387 .. 27400721
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1200300.v1.1.491

Sequence Viewer

Length: 489 bp
ATGTTGTTGCAACCAAAGCTGATGGCGCTGCAGCTTCGACCGCATCGAAACCTGGGTATGGGAGAGATGATGAGAGGTGCGGGAGGCCCTCTTCTATGCATAGGCGATCTGCTGAGCGACGTGGGGGAATCAGATGCTTCGCCGCCACCGCAACAACCTGAGACCTCTTCGTCTTCTTTTGTTTCCAGCTCCGATCAGAGCTTGGACCTCACCAAGTTGTTCCAGGAGAACTATGAGCAGCTGAATGAGGCGCTTGACGGTACAGATCATTCATGGGCAGCTCTTACTTTGAAGTTATGCAGTGCTTTGGAAACTGCAAACAAGTTAGTTCAGTCAACAAACTCAAATGTCATGTCAATGTCGGAGAAGGTTGGAGAGCTTGAGGGAGTTATCAAGAGGGCAGATTCTGCCATAACAGCAGCAAGGGTGGTTCATGGTTCACTAAACAAAAAGGAAGGACCACTTATTGGCAATCAAAATGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.31

Weight (kDa)

5.16

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 169
AccB7I CCANNNNNTGG 1 cut(s) 467
AciI CCGC 4 cut(s) 41, 80, 143, 149
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 2 cut(s) 58, 467
AgsI TTSAA 1 cut(s) 292
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 2 cut(s) 51, 222
AluBI AGCT 7 cut(s) 19, 34, 189, 201, 241, 281, 379
AluI AGCT 7 cut(s) 19, 34, 189, 201, 241, 281, 379
Alw26I GTCTC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 407
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 5 cut(s) 28, 31, 238, 278, 419
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AspLEI GCGC 2 cut(s) 28, 253
AspS9I GGNCC 3 cut(s) 86, 205, 458
AsuHPI GGTGA 1 cut(s) 202
AvaII GGWCC 2 cut(s) 205, 458
BbsI GAAGAC 1 cut(s) 165
BbvI GCAGC 5 cut(s) 15, 43, 250, 290, 431
BccI CCATC 1 cut(s) 16
BciT130I CCWGG 2 cut(s) 53, 224
BcoDI GTCTC 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 29
BfoI RGCGCY 2 cut(s) 29, 254
BisI GCNGC 6 cut(s) 29, 32, 143, 239, 279, 420
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 6 cut(s) 30, 33, 144, 240, 280, 421
Bme1390I CCNGG 2 cut(s) 53, 224
Bme18I GGWCC 2 cut(s) 205, 458
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 86, 205, 458
BmrFI CCNGG 2 cut(s) 53, 224
BmsI GCATC 2 cut(s) 52, 124
BpiI GAAGAC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 401
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 52
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 467
BseBI CCWGG 2 cut(s) 53, 224
BseDI CCNNGG 1 cut(s) 52
BseLI CCNNNNNNNGG 2 cut(s) 58, 467
BseMII CTCAG 2 cut(s) 104, 150
BseXI GCAGC 5 cut(s) 15, 43, 250, 290, 431
Bsh1285I CGRYCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 58, 467
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 1 cut(s) 87
Bso31I GGTCTC 1 cut(s) 155
Bsp143I GATC 3 cut(s) 106, 193, 265
Bsp1720I GCTNAGC 1 cut(s) 113
BspACI CCGC 4 cut(s) 41, 80, 143, 149
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 2 cut(s) 105, 151
BspMAI CTGCAG 1 cut(s) 33
BspTNI GGTCTC 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 3 cut(s) 106, 193, 265
Bst2UI CCWGG 2 cut(s) 53, 224
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 2 cut(s) 96, 172
BstAPI GCANNNNNTGC 1 cut(s) 407
BstDEI CTNAG 2 cut(s) 113, 159
BstH2I RGCGCY 2 cut(s) 29, 254
BstHHI GCGC 2 cut(s) 28, 253
BstKTI GATC 3 cut(s) 109, 196, 268
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 106, 193, 265
BstMCI CGRYCG 1 cut(s) 41
BstMWI GCNNNNNNNGC 6 cut(s) 16, 25, 40, 148, 407, 416
BstNI CCWGG 2 cut(s) 53, 224
BstSCI CCNGG 2 cut(s) 51, 222
BstSFI CTRYAG 1 cut(s) 29
BstV1I GCAGC 5 cut(s) 15, 43, 250, 290, 431
BstV2I GAAGAC 1 cut(s) 165
BsuRI GGCC 1 cut(s) 87
BtrI CACGTC 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 307
CaiI CAGNNNCTG 1 cut(s) 407
CfoI GCGC 2 cut(s) 28, 253
Cfr13I GGNCC 3 cut(s) 86, 205, 458
Csp6I GTAC 1 cut(s) 261
CviAII CATG 3 cut(s) 273, 352, 434
CviJI RGCY 8 cut(s) 19, 34, 87, 189, 201, 241, 281, 379
CviKI_1 RGCY 8 cut(s) 19, 34, 87, 189, 201, 241, 281, 379
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 2 cut(s) 113, 159
DpnI GATC 3 cut(s) 108, 195, 267
DpnII GATC 3 cut(s) 106, 193, 265
DrdI GACNNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 169
Eam1104I CTCTTC 2 cut(s) 96, 172
EarI CTCTTC 2 cut(s) 96, 172
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 205, 458
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 51, 222
EcoT22I ATGCAT 1 cut(s) 101
FaeI CATG 3 cut(s) 276, 355, 437
FaiI YATR 9 cut(s) 59, 97, 101, 234, 274, 298, 353, 413, 435
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FatI CATG 3 cut(s) 272, 351, 433
FauI CCCGC 1 cut(s) 73
Fnu4HI GCNGC 6 cut(s) 29, 32, 143, 239, 279, 420
Fsp4HI GCNGC 6 cut(s) 29, 32, 143, 239, 279, 420
GlaI GCGC 2 cut(s) 27, 252
GluI GCNGC 6 cut(s) 29, 32, 143, 239, 279, 420
HaeII RGCGCY 2 cut(s) 29, 254
HaeIII GGCC 1 cut(s) 87
HhaI GCGC 2 cut(s) 28, 253
Hin1II CATG 3 cut(s) 276, 355, 437
Hin6I GCGC 2 cut(s) 26, 251
HinP1I GCGC 2 cut(s) 26, 251
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 2 cut(s) 128, 404
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 2 cut(s) 336, 440
Hpy188I TCNGA 4 cut(s) 133, 193, 198, 364
Hpy188III TCNNGA 1 cut(s) 394
Hpy8I GTNNAC 2 cut(s) 336, 440
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 361, 449
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 5 cut(s) 10, 31, 99, 300, 317
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 25, 40, 148, 407, 416
HpyF3I CTNAG 2 cut(s) 113, 159
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 3 cut(s) 276, 355, 437
HspAI GCGC 2 cut(s) 26, 251
Kzo9I GATC 3 cut(s) 106, 193, 265
LmnI GCTCC 1 cut(s) 194
LpnPI CCDG 6 cut(s) 38, 65, 171, 199, 209, 236
Lsp1109I GCAGC 5 cut(s) 15, 43, 250, 290, 431
LweI GCATC 2 cut(s) 52, 124
MaeII ACGT 1 cut(s) 120
MalI GATC 3 cut(s) 108, 195, 267
MboI GATC 3 cut(s) 106, 193, 265
MboII GAAGA 3 cut(s) 83, 159, 165
MmeI TCCRAC 2 cut(s) 342, 352
MnlI CCTC 8 cut(s) 68, 77, 99, 175, 218, 241, 376, 390
Mph1103I ATGCAT 1 cut(s) 101
MslI CAYNNNNRTG 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 241
MspR9I CCNGG 2 cut(s) 53, 224
MvaI CCWGG 2 cut(s) 53, 224
MwoI GCNNNNNNNGC 6 cut(s) 16, 25, 40, 148, 407, 416
NdeII GATC 3 cut(s) 106, 193, 265
NlaIII CATG 3 cut(s) 276, 355, 437
NsiI ATGCAT 1 cut(s) 101
PcsI WCGNNNNNNNCGW 1 cut(s) 43
PfeI GAWTC 2 cut(s) 128, 404
PflMI CCANNNNNTGG 1 cut(s) 467
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 6 cut(s) 30, 33, 144, 240, 280, 421
Psp6I CCWGG 2 cut(s) 51, 222
PspGI CCWGG 2 cut(s) 51, 222
PspPI GGNCC 3 cut(s) 86, 205, 458
PstI CTGCAG 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 356
SatI GCNGC 6 cut(s) 29, 32, 143, 239, 279, 420
Sau3AI GATC 3 cut(s) 106, 193, 265
Sau96I GGNCC 3 cut(s) 86, 205, 458
ScrFI CCNGG 2 cut(s) 53, 224
SfaNI GCATC 2 cut(s) 52, 124
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 2 cut(s) 205, 458
SmiMI CAYNNNNRTG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SsiI CCGC 4 cut(s) 41, 80, 143, 149
StyD4I CCNGG 2 cut(s) 51, 222
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 1 cut(s) 123
TaqI TCGA 2 cut(s) 37, 46
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 2 cut(s) 128, 404
TscAI CASTG 1 cut(s) 307
TseI GCWGC 5 cut(s) 28, 31, 238, 278, 419
TspDTI ATGAA 2 cut(s) 261, 422
TspRI CASTG 1 cut(s) 307
Van91I CCANNNNNTGG 1 cut(s) 467
VpaK11BI GGWCC 2 cut(s) 205, 458
Zsp2I ATGCAT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.