Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011122

Physical Location & Seq

Genomic Coordinates Reverse (-)

28255815 .. 28260250

Gene Structure 4436 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1206600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2811 bp

ATGAGTTTCGCCGGACCCAGAATCTCAAAACCCGCAAAACCCCCAAACCCATCAGCCACAACGCCGCCGCAGCCGCAGCCGCAGCAACTTTCCTCTTCAAAGTCCTCTTCTTCTTCCGCTTCATCTTCCCTCTCGACCCACCTGGCCATGGTGGAGCTCAAGCAGAGAATCCTCACCTCCCTCTCGAAACTCTCCGATCGAGACACGTACCAAATCGCCGTCGAAGATCTCGAGAAAATTATCCAAACTCTCCCGCCGGAGAGCCTCCCTATGCTCCTCAACTGCCTCTACGATGCCTCCGCCGATCCGAAACCTGCCGTCAAAAAAGAGTCTTTGCGCCTGCTCTCATTGGTCTCTGCTTCCCATCCCGATTTCACATCCACCCATTTGACCAAAATCATTGCCCACGTTGTCAAGAGGCTCAAGGACGCCGATTCCGGCGTCAGGGACGCGTGCCGCGACTCCATTGGCTCGCTCTCGGCGCAGTACTTGAAGGCAGATAGTGTGAGCGACAATGGGTCGGTTGTGGGTTTATTTATGAAGCCTTTGTTTGAGGCTATGATGGAGCAGAACAAAGGGGTGCAATCTGGTGCGGCATTGTGCATGGCTAAGATTGTGGACTGCGCTTTAGAACCGCCTGTTTCGTCTTTTCAAAAGCTGTGTCCAAGGATCTGCAAGTTGCTCAACAATCCTAACTTCTTGGCTAAGTCATCGCTCTTGCCGGTTGTTTCGAGCCTGTCTCAGGTTGGAGCGATTGCGCCCCAAAGCTTGGATAATCTACAGCAAAGTATTCATGACTGCCTTGGAAGCCCAGATTGGGCAACACGTAAAGCAGCAGCCGATGTGCTGATTGCATTAGCATTGCATTCAAGCAACATGATTACAGATAGAACTGCTTCCACCCTGGCTGTGCTTGAGGCTTGCCGTTTTGACAAGATTAAACCTGTTAGAGATAGCATGACAGAGGCATTGCAGTTTTGGAAAAAGATAGCTGGGAAAGGAGGAGATGAAGCTCCAAATGAAAAGAAAGTCCCGTCTCATACTGAGTTGTCAGAGAAGAATGAGCCAAAAAATTCAAAGGCTGGTGAGAGAATAGAGCAAGGAGCAAAGGGTTCATCTAATGGTTCATCCCCTACTTCAGATTCTGTTTCTAAATCCAAAGCCAGTGGAGATAAAGTGGTTGTAGCTTTGAAGAAGAAACCACCTGTCTTAACTGATAAAGAGCTAAACCCAGAATTCTTCCAGAAACTTGAAAAAAGGGTTTCTGATGATTTGCCTGTTGAGGTAGTTGTTCCTCGTAGACACGTCAATTCTTCAAACTCAAATAATGAGGTGGAACCAGAGGCAAATGATACAGATTCCAAGGAAACTTTAAACCGGATTGCAAACAGCCAATCTGATGATATTCATGGATCTTTCAGTAGTAAATACCGTAACATAGAGAGAGGATTAGCTGGTCTCTATACTAAACAACGAGATCATGATGACCTTGCACGAGATAAATTTCCAGAAGAAAGGGTGAATGGAAAAGACTCCAGAACAAGGGCAGTTGATATTGATGAGAGGATTGAGATAAATCAAAGGGAGTTGTCCACTAGTCGTGCAGGTTTCTCCAAAACTGATGGTCAACCTGAAGGATCCTTTGTCAATAACAAAGGAAATTGGCTGGCTATCCAAAGGCAATTGTTGCAGCTGGAGAGACAACAAGGTCATCTGATGAGCATGTTGCAGGATTTCATGGGCGGTTCTCATGACAGTATGGTAACTTTGGAGAACAGAGTAAGGGGTCTTGAGAGAGTTGTTGAGGACTTGGCAAGAGATTTATCAATATCATCAGGTAGAAGAGGCGGTAATTTTTCAATGGGATATGAGGGGGCATCTAATAGGCCTTTAGGTAAGTACAACGGTTTTCCGGATTACACTAGTAACAAGTTTGGAAGAGGTGGAGATGGACGAATTCTGTTTGGCGAAAGATTTTCCCAAAATGATGGACATGTTTCCGGCATGAGGGGGCGGGGCCCTACTTGGAGATCTGACATGTCTGAGGTGTGGGATTTTTCAACATATGGTGGTTCCAGAAATGGACAGACTAGTTCGAGGAAGGCCGTAGGAGGTGGTCCTGTGGATGGTAGGTCACCCAAATCAGAGAATGGGAGTGACCAGGGTAACAGCAGGCGAGCATGGGATAAAGGGGCTGGACCTGTAAGGTTTGGTGAGGGGCCTTCCGCAAGAAGTGTTTGGCAAGCTTCAAAAGATGAAGCTACCTTGGAAGCAATTCGTGTGGCTGGAGAGGACAATGGAACATCTAGAGCAGGAAGAGTAGCTATACCTGAATTGACTGCAGAGGCTATGGAAGATGATACTGTGGGCCAAGAGCGCAATCCAATTTGGAATTCTTGGACTAATGCAATGGATGCTCTTCAAGTGGGTGATGTGGATACGGCCTTTGTGGAAGTATTGTCTACAGGAGATGATCTCTTGCTTGTAAAGTTAATGGACAGATCAGGTCCTGCGATTGACCAACTTTCAAATGAGACAGCAACTGAGGTCTTGCATGCCGTTGGGCAATTTTTACCAGAGCCAAACTTGTTTGATGTCTGTTTGGCTTGGATTCAGCAGTTGGTTGAAATAGTGCTGGAAAATGGATCTGATGTTTTCGGCCTTCCCGCTGACGTAAAGAAGGAACTCGTTTTGAATCTGCATGAGGCTTCTGTAAATCCACCCGAGGACTGGGAAGGTGCAACGCCGGATCAACTTTTGGTGCAGTTGGCATCATCCTGGGGAATAAATCCGCAGCATCATGACAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009653 GO:0009826 GO:0009987 GO:0010005 GO:0010031 GO:0015630 GO:0015631 GO:0016043 GO:0016049 GO:0030863 GO:0030981 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050879 GO:0055028 GO:0060560 GO:0071840 GO:0071944 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

937

Amino Acids

101.8

Weight (kDa)

5.79

Isoelectric Point (pI)

39.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOR1L1_N	PF24714	51 - 329	4.2e-121	TORTIFOLIA1 N-terminal
TOR1L1_C	PF24713	796 - 927	1.4e-62	TORTIFOLIA1 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27060
fragaria_vesca	FvH4_3g22240
malus_domestica	MD03G1206600.v1.1 MD11G1222000.v1.1
prunus_persica	Prupe.4G209400_v2.0.a1 Prupe.4G209400_v2.0.a1
pyrus_communis	pycom03g15640 pycom11g19580
rosa_chinensis	RchiOBHm_Chr5g0038291
rosa_laevigata	RLG00000033844
rosa_multiflora	Rmu_sc0000764.1_g000003 Rmu_sc0003858.1_g000004 Rmu_sc0035524.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042730
rosa_rugosa	Rorug05G0171800
rosa_samantha	Rh5AG259100 Rh5BG262900 Rh5CG295300 Rh5DG271900
rosa_wichuraiana	Rw5G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1707
Acc36I	ACCTGC	2 cut(s)	322, 1595
AccB7I	CCANNNNNTGG	1 cut(s)	769
AccI	GTMKAC	2 cut(s)	1300, 2462
AccII	CGCG	2 cut(s)	452, 459
AccIII	TCCGGA	1 cut(s)	1912
AclWI	GGATC	7 cut(s)	299, 677, 1420, 1632, 1645, 2653, 2757
AcoI	YGGCCR	1 cut(s)	144
AcsI	RAATTY	5 cut(s)	1072, 1235, 1502, 1956, 2392
AcuI	CTGAAG	2 cut(s)	1122, 1653
AcyI	GRCGYC	2 cut(s)	429, 441
AfaI	GTAC	3 cut(s)	209, 488, 1901
AfiI	CCNNNNNNNGG	9 cut(s)	148, 444, 742, 769, 817, 1542, 2007, 2126, 2730
AflIII	ACRYGT	6 cut(s)	204, 450, 824, 1303, 1993, 2037
AhdI	GACNNNNNGTC	1 cut(s)	517
AhlI	ACTAGT	3 cut(s)	1595, 1922, 2090
AjiI	CACGTC	1 cut(s)	1306
AjnI	CCWGG	4 cut(s)	141, 903, 2160, 2777
AjuI	GAANNNNNNNTTGG	4 cut(s)	799, 831, 2377, 2409
Alw21I	GWGCWC	1 cut(s)	159
Alw26I	GTCTC	7 cut(s)	195, 358, 744, 1041, 1463, 1693, 2528
AlwI	GGATC	7 cut(s)	299, 677, 1420, 1632, 1645, 2653, 2757
AlwNI	CAGNNNCTG	3 cut(s)	1145, 2510, 2543
Ama87I	CYCGRG	2 cut(s)	230, 2723
Aor13HI	TCCGGA	1 cut(s)	1912
AoxI	GGCC	8 cut(s)	144, 1886, 2017, 2103, 2219, 2368, 2442, 2659
ApaI	GGGCCC	1 cut(s)	2021
ApeKI	GCWGC	7 cut(s)	70, 76, 82, 833, 836, 1690, 2794
ApoI	RAATTY	5 cut(s)	1072, 1235, 1502, 1956, 2392
ArsI	GACNNNNNNTTYG	2 cut(s)	646, 678
Asp700I	GAANNNNTTC	2 cut(s)	895, 2274
AspLEI	GCGC	5 cut(s)	339, 484, 626, 760, 2379
AspS9I	GGNCC	8 cut(s)	14, 2017, 2018, 2117, 2198, 2219, 2368, 2507
AsuHPI	GGTGA	6 cut(s)	166, 1097, 1531, 2127, 2225, 2441
AvaI	CYCGRG	2 cut(s)	230, 2723
AvaII	GGWCC	4 cut(s)	14, 2117, 2198, 2507
BaeGI	GKGCMC	1 cut(s)	2021
BalI	TGGCCA	1 cut(s)	146
BamHI	GGATCC	1 cut(s)	1637
BanII	GRGCYC	2 cut(s)	159, 2021
BauI	CACGAG	1 cut(s)	1494
Bbv12I	GWGCWC	1 cut(s)	159
BbvI	GCAGC	7 cut(s)	82, 88, 94, 845, 848, 1702, 2806
BccI	CCATC	7 cut(s)	58, 372, 556, 1616, 1943, 1982, 2120
BceAI	ACGGC	6 cut(s)	203, 302, 909, 2090, 2457, 2543
BciT130I	CCWGG	4 cut(s)	143, 905, 2162, 2779
BciVI	GTATCC	1 cut(s)	2431
BcoDI	GTCTC	7 cut(s)	195, 358, 744, 1041, 1463, 1693, 2528
BcuI	ACTAGT	3 cut(s)	1595, 1922, 2090
BfaI	CTAG	4 cut(s)	1596, 1923, 2091, 2307
BfmI	CTRYAG	3 cut(s)	779, 2340, 2463
BfuAI	ACCTGC	2 cut(s)	322, 1595
BfuI	GTATCC	1 cut(s)	2431
BglII	AGATCT	2 cut(s)	226, 2030
BmcAI	AGTACT	1 cut(s)	488
Bme1390I	CCNGG	4 cut(s)	143, 905, 2162, 2779
Bme18I	GGWCC	4 cut(s)	14, 2117, 2198, 2507
BmeRI	GACNNNNNGTC	1 cut(s)	517
BmeT110I	CYCGRG	2 cut(s)	230, 2723
BmgBI	CACGTC	1 cut(s)	1306
BmgT120I	GGNCC	8 cut(s)	14, 2017, 2018, 2117, 2198, 2219, 2368, 2507
BmiI	GGNNCC	7 cut(s)	16, 1338, 1639, 2018, 2019, 2074, 2220
BmrFI	CCNGG	4 cut(s)	143, 905, 2162, 2779
BmrI	ACTGGG	1 cut(s)	2740
BmsI	GCATC	5 cut(s)	283, 1886, 2404, 2780, 2806
BmuI	ACTGGG	1 cut(s)	2740
BplI	GAGNNNNNCTC	4 cut(s)	724, 756, 2460, 2492
BpmI	CTGGAG	3 cut(s)	1519, 1715, 2307
BpuEI	CTTGAG	4 cut(s)	143, 407, 935, 1811
BsaAI	YACGTR	2 cut(s)	207, 827
BsaHI	GRCGYC	2 cut(s)	429, 441
BsaI	GGTCTC	2 cut(s)	358, 1463
BsaJI	CCNNGG	9 cut(s)	147, 665, 802, 903, 1362, 2161, 2265, 2724, 2778
BsaWI	WCCGGW	2 cut(s)	1377, 1912
BsaXI	ACNNNNNCTCC	4 cut(s)	281, 311, 1161, 1191
Bsc4I	CCNNNNNNNGG	9 cut(s)	148, 444, 742, 769, 817, 1542, 2007, 2126, 2730
Bse118I	RCCGGY	1 cut(s)	721
Bse1I	ACTGG	2 cut(s)	1164, 2735
Bse3DI	GCAATG	4 cut(s)	399, 860, 968, 2415
BseAI	TCCGGA	1 cut(s)	1912
BseBI	CCWGG	4 cut(s)	143, 905, 2162, 2779
BseDI	CCNNGG	9 cut(s)	147, 665, 802, 903, 1362, 2161, 2265, 2724, 2778
BseGI	GGATG	6 cut(s)	364, 377, 1127, 2131, 2419, 2774
BseLI	CCNNNNNNNGG	9 cut(s)	148, 444, 742, 769, 817, 1542, 2007, 2126, 2730
BseMI	GCAATG	4 cut(s)	399, 860, 968, 2415
BseMII	CTCAG	4 cut(s)	755, 1035, 2034, 2535
BseNI	ACTGG	2 cut(s)	1164, 2735
BseRI	GAGGAG	2 cut(s)	266, 1017
BseSI	GKGCMC	1 cut(s)	2021
BseXI	GCAGC	7 cut(s)	82, 88, 94, 845, 848, 1702, 2806
BseYI	CCCAGC	1 cut(s)	992
BsgI	GTGCAG	2 cut(s)	1623, 2783
Bsh1236I	CGCG	2 cut(s)	452, 459
Bsh1285I	CGRYCG	1 cut(s)	199
BshFI	GGCC	8 cut(s)	146, 1888, 2019, 2105, 2221, 2370, 2444, 2661
BsiEI	CGRYCG	1 cut(s)	199
BsiHKAI	GWGCWC	1 cut(s)	159
BsiHKCI	CYCGRG	2 cut(s)	230, 2723
BsiSI	CCGG	8 cut(s)	12, 257, 438, 722, 1378, 1913, 2001, 2747
BslFI	GGGAC	2 cut(s)	461, 1016
BslI	CCNNNNNNNGG	9 cut(s)	148, 444, 742, 769, 817, 1542, 2007, 2126, 2730
BsmAI	GTCTC	7 cut(s)	195, 358, 744, 1041, 1463, 1693, 2528
BsmBI	CGTCTC	1 cut(s)	1041
BsmFI	GGGAC	2 cut(s)	461, 1016
BsmI	GAATGC	1 cut(s)	865
BsnI	GGCC	8 cut(s)	146, 1888, 2019, 2105, 2221, 2370, 2444, 2661
Bso31I	GGTCTC	2 cut(s)	358, 1463
BsoBI	CYCGRG	2 cut(s)	230, 2723
Bsp120I	GGGCCC	1 cut(s)	2017
Bsp1286I	GDGCHC	2 cut(s)	159, 2021
Bsp13I	TCCGGA	1 cut(s)	1912
Bsp19I	CCATGG	1 cut(s)	147
BspANI	GGCC	8 cut(s)	146, 1888, 2019, 2105, 2221, 2370, 2444, 2661
BspCNI	CTCAG	4 cut(s)	754, 1036, 2035, 2536
BspEI	TCCGGA	1 cut(s)	1912
BspFNI	CGCG	2 cut(s)	452, 459
BspHI	TCATGA	4 cut(s)	793, 1480, 1750, 2800
BspLI	GGNNCC	7 cut(s)	16, 1338, 1639, 2018, 2019, 2074, 2220
BspMAI	CTGCAG	1 cut(s)	2344
BspMI	ACCTGC	2 cut(s)	322, 1595
BspPI	GGATC	7 cut(s)	299, 677, 1420, 1632, 1645, 2653, 2757
BspQI	GCTCTTC	1 cut(s)	2424
BspTNI	GGTCTC	2 cut(s)	358, 1463
BsrDI	GCAATG	4 cut(s)	399, 860, 968, 2415
BsrFI	RCCGGY	1 cut(s)	721
BsrI	ACTGG	2 cut(s)	1164, 2735
BssAI	RCCGGY	1 cut(s)	721
BssECI	CCNNGG	9 cut(s)	147, 665, 802, 903, 1362, 2161, 2265, 2724, 2778
BssNI	GRCGYC	2 cut(s)	429, 441
BssSI	CACGAG	1 cut(s)	1494
BssT1I	CCWWGG	5 cut(s)	147, 665, 802, 1362, 2265
Bst2BI	CACGAG	1 cut(s)	1494
Bst2UI	CCWGG	4 cut(s)	143, 905, 2162, 2779
Bst4CI	ACNGT	4 cut(s)	1433, 1757, 1907, 2365
Bst6I	CTCTTC	6 cut(s)	100, 112, 1837, 1933, 2311, 2424
BstACI	GRCGYC	2 cut(s)	429, 441
BstAPI	GCANNNNNTGC	2 cut(s)	1687, 2414
BstBAI	YACGTR	2 cut(s)	207, 827
BstC8I	GCNNGC	9 cut(s)	341, 454, 473, 922, 1668, 2174, 2178, 2244, 2556
BstDEI	CTNAG	6 cut(s)	609, 705, 741, 1044, 2043, 2544
BstDSI	CCRYGG	1 cut(s)	147
BstEII	GGTNACC	1 cut(s)	2133
BstENI	CCTNNNNNAGG	1 cut(s)	740
BstF5I	GGATG	6 cut(s)	364, 377, 1127, 2131, 2419, 2774
BstFNI	CGCG	2 cut(s)	452, 459
BstHHI	GCGC	5 cut(s)	339, 484, 626, 760, 2379
BstMAI	GTCTC	7 cut(s)	195, 358, 744, 1041, 1463, 1693, 2528
BstMCI	CGRYCG	1 cut(s)	199
BstNI	CCWGG	4 cut(s)	143, 905, 2162, 2779
BstNSI	RCATGY	4 cut(s)	1726, 1997, 2041, 2558
BstPI	GGTNACC	1 cut(s)	2133
BstSCI	CCNGG	4 cut(s)	141, 903, 2160, 2777
BstSFI	CTRYAG	3 cut(s)	779, 2340, 2463
BstSLI	GKGCMC	1 cut(s)	2021
BstUI	CGCG	2 cut(s)	452, 459
BstV1I	GCAGC	7 cut(s)	82, 88, 94, 845, 848, 1702, 2806
BstX2I	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2030, 2645
BstXI	CCANNNNNNTGG	1 cut(s)	1988
BstYI	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2030, 2645
BsuI	GTATCC	1 cut(s)	2431
BsuRI	GGCC	8 cut(s)	146, 1888, 2019, 2105, 2221, 2370, 2444, 2661
BtgI	CCRYGG	1 cut(s)	147
BtgZI	GCGATG	1 cut(s)	696
BtrI	CACGTC	1 cut(s)	1306
BtsCI	GGATG	6 cut(s)	364, 377, 1127, 2131, 2419, 2774
BtsIMutI	CAGTG	1 cut(s)	1171
BveI	ACCTGC	2 cut(s)	322, 1595
Cac8I	GCNNGC	9 cut(s)	341, 454, 473, 922, 1668, 2174, 2178, 2244, 2556
CaiI	CAGNNNCTG	3 cut(s)	1145, 2510, 2543
CciI	TCATGA	4 cut(s)	793, 1480, 1750, 2800
CfoI	GCGC	5 cut(s)	339, 484, 626, 760, 2379
Cfr10I	RCCGGY	1 cut(s)	721
Cfr13I	GGNCC	8 cut(s)	14, 2017, 2018, 2117, 2198, 2219, 2368, 2507
CseI	GACGC	3 cut(s)	430, 437, 458
Csp6I	GTAC	3 cut(s)	208, 487, 1900
CspCI	CAANNNNNGTGG	4 cut(s)	1147, 1182, 2262, 2297
CviQI	GTAC	3 cut(s)	208, 487, 1900
DdeI	CTNAG	6 cut(s)	609, 705, 741, 1044, 2043, 2544
DraI	TTTAAA	1 cut(s)	1374
DrdI	GACNNNNNNGTC	1 cut(s)	1707
DriI	GACNNNNNGTC	1 cut(s)	517
DseDI	GACNNNNNNGTC	1 cut(s)	1707
EaeI	YGGCCR	1 cut(s)	144
Eam1104I	CTCTTC	6 cut(s)	100, 112, 1837, 1933, 2311, 2424
Eam1105I	GACNNNNNGTC	1 cut(s)	517
EarI	CTCTTC	6 cut(s)	100, 112, 1837, 1933, 2311, 2424
EciI	GGCGGA	1 cut(s)	289
Ecl136II	GAGCTC	1 cut(s)	157
Eco130I	CCWWGG	5 cut(s)	147, 665, 802, 1362, 2265
Eco147I	AGGCCT	1 cut(s)	1888
Eco24I	GRGCYC	2 cut(s)	159, 2021
Eco31I	GGTCTC	2 cut(s)	358, 1463
Eco47I	GGWCC	4 cut(s)	14, 2117, 2198, 2507
Eco53kI	GAGCTC	1 cut(s)	157
Eco57I	CTGAAG	2 cut(s)	1122, 1653
Eco88I	CYCGRG	2 cut(s)	230, 2723
Eco91I	GGTNACC	1 cut(s)	2133
EcoICRI	GAGCTC	1 cut(s)	157
EcoNI	CCTNNNNNAGG	1 cut(s)	740
EcoO109I	RGGNCCY	4 cut(s)	2017, 2018, 2219, 2507
EcoO65I	GGTNACC	1 cut(s)	2133
EcoRI	GAATTC	3 cut(s)	1235, 1956, 2392
EcoRII	CCWGG	4 cut(s)	141, 903, 2160, 2777
EcoT14I	CCWWGG	5 cut(s)	147, 665, 802, 1362, 2265
EcoT38I	GRGCYC	2 cut(s)	159, 2021
ErhI	CCWWGG	5 cut(s)	147, 665, 802, 1362, 2265
Esp3I	CGTCTC	1 cut(s)	1041
FaqI	GGGAC	2 cut(s)	461, 1016
FauI	CCCGC	4 cut(s)	40, 261, 2007, 2674
FauNDI	CATATG	1 cut(s)	2065
FblI	GTMKAC	2 cut(s)	1300, 2462
FokI	GGATG	6 cut(s)	351, 364, 1114, 2138, 2426, 2761
FriOI	GRGCYC	2 cut(s)	159, 2021
FspBI	CTAG	4 cut(s)	1596, 1923, 2091, 2307
GlaI	GCGC	5 cut(s)	338, 483, 625, 759, 2378
GsaI	CCCAGC	1 cut(s)	996
GsuI	CTGGAG	3 cut(s)	1519, 1715, 2307
HaeIII	GGCC	8 cut(s)	146, 1888, 2019, 2105, 2221, 2370, 2444, 2661
HapII	CCGG	8 cut(s)	12, 257, 438, 722, 1378, 1913, 2001, 2747
HgaI	GACGC	3 cut(s)	430, 437, 458
HhaI	GCGC	5 cut(s)	339, 484, 626, 760, 2379
Hin1I	GRCGYC	2 cut(s)	429, 441
Hin6I	GCGC	5 cut(s)	337, 482, 624, 758, 2377
HinP1I	GCGC	5 cut(s)	337, 482, 624, 758, 2377
HincII	GTYRAC	1 cut(s)	1628
HindII	GTYRAC	1 cut(s)	1628
HindIII	AAGCTT	2 cut(s)	766, 2244
HpaII	CCGG	8 cut(s)	12, 257, 438, 722, 1378, 1913, 2001, 2747
HphI	GGTGA	6 cut(s)	166, 1097, 1531, 2127, 2225, 2441
Hpy166II	GTNNAC	5 cut(s)	619, 1301, 1593, 1628, 2463
Hpy8I	GTNNAC	5 cut(s)	619, 1301, 1593, 1628, 2463
Hpy99I	CGWCG	1 cut(s)	224
HpyAV	CCTTC	7 cut(s)	487, 1628, 2095, 2232, 2672, 2674, 2729
HpyCH4III	ACNGT	4 cut(s)	1433, 1757, 1907, 2365
HpyCH4IV	ACGT	5 cut(s)	206, 408, 826, 1305, 2673
HpyF3I	CTNAG	6 cut(s)	609, 705, 741, 1044, 2043, 2544
HpySE526I	ACGT	5 cut(s)	206, 408, 826, 1305, 2673
Hsp92I	GRCGYC	2 cut(s)	429, 441
HspAI	GCGC	5 cut(s)	337, 482, 624, 758, 2377
Kpn2I	TCCGGA	1 cut(s)	1912
LguI	GCTCTTC	1 cut(s)	2424
LmnI	GCTCC	6 cut(s)	154, 279, 565, 749, 1018, 1103
Lsp1109I	GCAGC	7 cut(s)	82, 88, 94, 845, 848, 1702, 2806
LweI	GCATC	5 cut(s)	283, 1886, 2404, 2780, 2806
MaeI	CTAG	4 cut(s)	1596, 1923, 2091, 2307
MaeII	ACGT	5 cut(s)	206, 408, 826, 1305, 2673
MaeIII	GTNAC	6 cut(s)	1433, 1762, 1925, 2133, 2156, 2165
MfeI	CAATTG	1 cut(s)	1682
MflI	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2030, 2645
MhlI	GDGCHC	2 cut(s)	159, 2021
MlsI	TGGCCA	1 cut(s)	146
MluI	ACGCGT	1 cut(s)	450
MluNI	TGGCCA	1 cut(s)	146
MlyI	GAGTC	3 cut(s)	338, 455, 1526
MmeI	TCCRAC	1 cut(s)	727
Mox20I	TGGCCA	1 cut(s)	146
MroI	TCCGGA	1 cut(s)	1912
MroXI	GAANNNNTTC	2 cut(s)	895, 2274
MscI	TGGCCA	1 cut(s)	146
MseI	TTAA	4 cut(s)	939, 1211, 1373, 2492
Msp20I	TGGCCA	1 cut(s)	146
MspA1I	CMGCKG	2 cut(s)	1693, 2669
MspI	CCGG	8 cut(s)	12, 257, 438, 722, 1378, 1913, 2001, 2747
MspR9I	CCNGG	4 cut(s)	143, 905, 2162, 2779
MunI	CAATTG	1 cut(s)	1682
Mva1269I	GAATGC	1 cut(s)	865
MvaI	CCWGG	4 cut(s)	143, 905, 2162, 2779
MvnI	CGCG	2 cut(s)	452, 459
NcoI	CCATGG	1 cut(s)	147
NdeI	CATATG	1 cut(s)	2065
NlaIV	GGNNCC	7 cut(s)	16, 1338, 1639, 2018, 2019, 2074, 2220
NmeAIII	GCCGAG	1 cut(s)	458
NmuCI	GTSAC	2 cut(s)	2133, 2156
NspI	RCATGY	4 cut(s)	1726, 1997, 2041, 2558
PaeI	GCATGC	1 cut(s)	2558
PaeR7I	CTCGAG	1 cut(s)	230
PagI	TCATGA	4 cut(s)	793, 1480, 1750, 2800
PceI	AGGCCT	1 cut(s)	1888
PciI	ACATGT	2 cut(s)	1993, 2037
PciSI	GCTCTTC	1 cut(s)	2424
PcsI	WCGNNNNNNNCGW	2 cut(s)	228, 456
PctI	GAATGC	1 cut(s)	865
PdmI	GAANNNNTTC	2 cut(s)	895, 2274
PfeI	GAWTC	7 cut(s)	21, 168, 434, 1142, 1358, 2611, 2695
PflMI	CCANNNNNTGG	1 cut(s)	769
Ple19I	CGATCG	1 cut(s)	199
PleI	GAGTC	3 cut(s)	337, 455, 1526
PpsI	GAGTC	3 cut(s)	337, 455, 1526
Ppu21I	YACGTR	2 cut(s)	207, 827
PpuMI	RGGWCCY	1 cut(s)	2507
PscI	ACATGT	2 cut(s)	1993, 2037
Psp124BI	GAGCTC	1 cut(s)	159
Psp5II	RGGWCCY	1 cut(s)	2507
Psp6I	CCWGG	4 cut(s)	141, 903, 2160, 2777
PspEI	GGTNACC	1 cut(s)	2133
PspFI	CCCAGC	1 cut(s)	992
PspGI	CCWGG	4 cut(s)	141, 903, 2160, 2777
PspN4I	GGNNCC	7 cut(s)	16, 1338, 1639, 2018, 2019, 2074, 2220
PspOMI	GGGCCC	1 cut(s)	2017
PspPI	GGNCC	8 cut(s)	14, 2017, 2018, 2117, 2198, 2219, 2368, 2507
PspPPI	RGGWCCY	1 cut(s)	2507
PstI	CTGCAG	1 cut(s)	2344
PstNI	CAGNNNCTG	3 cut(s)	1145, 2510, 2543
PsuI	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2030, 2645
PvuI	CGATCG	1 cut(s)	199
PvuII	CAGCTG	1 cut(s)	1693
RsaI	GTAC	3 cut(s)	209, 488, 1901
RsaNI	GTAC	3 cut(s)	208, 487, 1900
SacI	GAGCTC	1 cut(s)	159
SapI	GCTCTTC	1 cut(s)	2424
SaqAI	TTAA	4 cut(s)	939, 1211, 1373, 2492
Sau96I	GGNCC	8 cut(s)	14, 2017, 2018, 2117, 2198, 2219, 2368, 2507
ScaI	AGTACT	1 cut(s)	488
SchI	GAGTC	3 cut(s)	338, 455, 1526
ScrFI	CCNGG	4 cut(s)	143, 905, 2162, 2779
SduI	GDGCHC	2 cut(s)	159, 2021
SfaNI	GCATC	5 cut(s)	283, 1886, 2404, 2780, 2806
SfcI	CTRYAG	3 cut(s)	779, 2340, 2463
Sfr274I	CTCGAG	1 cut(s)	230
SinI	GGWCC	4 cut(s)	14, 2117, 2198, 2507
SlaI	CTCGAG	1 cut(s)	230
SmlI	CTYRAG	5 cut(s)	158, 230, 422, 914, 1790
SmoI	CTYRAG	5 cut(s)	158, 230, 422, 914, 1790
SpeI	ACTAGT	3 cut(s)	1595, 1922, 2090
SphI	GCATGC	1 cut(s)	2558
SseBI	AGGCCT	1 cut(s)	1888
SspMI	CTAG	4 cut(s)	1596, 1923, 2091, 2307
SstI	GAGCTC	1 cut(s)	159
StuI	AGGCCT	1 cut(s)	1888
StyD4I	CCNGG	4 cut(s)	141, 903, 2160, 2777
StyI	CCWWGG	5 cut(s)	147, 665, 802, 1362, 2265
TaaI	ACNGT	4 cut(s)	1433, 1757, 1907, 2365
TaiI	ACGT	5 cut(s)	209, 411, 829, 1308, 2676
TaqI	TCGA	7 cut(s)	134, 185, 199, 222, 231, 731, 2096
TatI	WGTACW	2 cut(s)	486, 1899
TauI	GCSGC	6 cut(s)	67, 70, 76, 82, 459, 596
TfiI	GAWTC	7 cut(s)	21, 168, 434, 1142, 1358, 2611, 2695
Tru1I	TTAA	4 cut(s)	939, 1211, 1373, 2492
Tru9I	TTAA	4 cut(s)	939, 1211, 1373, 2492
TscAI	CASTG	1 cut(s)	1171
TseFI	GTSAC	2 cut(s)	2133, 2156
TseI	GCWGC	7 cut(s)	70, 76, 82, 833, 836, 1690, 2794
Tsp45I	GTSAC	2 cut(s)	2133, 2156
TspRI	CASTG	1 cut(s)	1171
Van91I	CCANNNNNTGG	1 cut(s)	769
VpaK11BI	GGWCC	4 cut(s)	14, 2117, 2198, 2507
XagI	CCTNNNNNAGG	1 cut(s)	740
XapI	RAATTY	5 cut(s)	1072, 1235, 1502, 1956, 2392
XbaI	TCTAGA	1 cut(s)	2306
XceI	RCATGY	4 cut(s)	1726, 1997, 2041, 2558
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2727
XhoI	CTCGAG	1 cut(s)	230
XmiI	GTMKAC	2 cut(s)	1300, 2462
XmnI	GAANNNNTTC	2 cut(s)	895, 2274
XspI	CTAG	4 cut(s)	1596, 1923, 2091, 2307
ZrmI	AGTACT	1 cut(s)	488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.