Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0011122

Physical Location & Seq

Genomic Coordinates Reverse (-)

32516428 .. 32520917

Gene Structure 4490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1222000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2817 bp

ATGAGTTTTGCCGGACCCAGAATTTCCAAACCGGCTAAGCCCCCAAACCCATCACCCACAACGCCGCCGCAGCCGCAGCAACCACCAAACTCCTCTTCAAAGTCCTCTGCTTCTTCCGCTTCTTCTTCCCTCTCGACCCACCTGGCCATGGTGGAGCTCAAGCAGAGAATCCTCACCTCCCTCTCCAAACTCTCCGACCGAGACACGTACCAAATTGCCGTTGAAGATCTCGAGAAAATCATCCAAACCCTCCCGCCGGAGGGCCTCCCTATGCTCCTCAACTGCCTCTACGACGCCTCCGCCGATCCGAAACCCGCCGTCAAGAAAGAGTCTTTGCGCCTGCTCGCATTGGTCTCTGCTTCCCACCCCGATTTCACCTCCACCCATTTGACCAAAATCATTGCCCACATCGTCAAGAGGCTCAAGGATGCCGATTCCGGCGTCCGGGACGCGTGCCGCGACGCCATTGGCGCGCTGTCGGCGCAGTACTTGAAGGCAGAGAGTGTGAGCGACAATGGGTCGGTTGTGGGTTTGTTTACGAAGCCTTTGTTTGAGGCTATGGCCGAGCAGAACAAAGGGGTGCAATCTGGTGCGGCACTGTGTATGGCTAAGATTGTAGACTGCGCTTTAGACCCGCCTGTTTCGTCTTTTCAAAGGCTGTTTCCAAGGATCTGCAAGTTGCTTAACAATCCTAACTTCTTGGCTAAGGCATCGCTCTTGCCGGTTGTTTCGAGCCTGTCTCAGGTTGGAGCGATTGCGCCCCAAAGCTTGGATAATCTACTGCAAAGTATTCATGAATGCCTTGGGAGCACAGATTGGGCAACACGTAAAGCAGCAGCTGATGTGCTGGTTGCATTAGCATTGCATTCGAGCAACTTGATTACAGATAGAACCGCTTCCACCCTGACTGTCCTCGAGTCTTGCCGTTTTGACAAGATTAAACCTGTCAGAGATAGCATGACAGAGGCATTGCAGTTCTGGAAAAAGATAGCTGGGAAAGGAGAAGATGAAGCTCCAAATGACAAGAAAGTCTCGTCTCATGCTGAGTTGTCAGAGAAGAATGAGCCAAAAAATTCAAAGCCTAGTGAGAAAACAGATCAAGGAGCAAAGGGTTCATCTAATGGTTCATCTCCTACTTCAGATTCTGTTTCTAAATCCAAAGCCAGTGGAGATAAAGCGGGTGTAGCTTTGAAGAAGAAACTGCCCATCTTAACTGACAAAGAGCTAAACCCAGAATTCTTCCAGAAACTTGAAAAGAGGGTTTCTGATGATTTGCCCGTTGAGGTAGTTGTTCCTCGTAGACATGTCAATTCTTCAAACTCAAATAATGAGGTAGAACCAGAGGCAAATGATACAAATTCCAAAGAAACTCAAAACCGGATTGCAAACAGCCAATTTAATGATATTCATGGATCTTTCAGTAGTAAATACCGTAACACAGAGAGAGGAATAGCTGGTCTGTATACTAAACAACGAGATCATGATGACCTTGCGAGGGATAAATGGCCAGAAGAAAGGGCGAATGGAAAAGACTCCAGAACGAGAGCAGTTGATATTGATGACAGGATTGATATAAATCAAAGGGAGTCGTCCAGTAGTCGTGCAGGTTTCTCTAAACCCGATGGTCAACCTGAAGGATCCTTTGTCAATAACAAAGGAAATTGGTTGGCTATCCAAAGGCAATTGTTGCAGCTGGAGAGACAACAAGGTCATCTTATGAGCATGTTGCAGGATTTCATGGGCGGGTCTCATGACAGCATGATAACTTTGGAGAACAGAGTAAGGGGTCTTGAGAGAGTTGTTGAAGACTTGGCACGAGATTTATCAATATCATCAGGTAGAAGAGGCGGTAATTTTTCGATGGGGTATGAGGGGTCTTCTAATAGGCCTTTAGGCAAGTATAACGGCTTTCCTGATTACACTGGTAACAAGTTTGGAAGAGGTGGAGATGGACGAATTCCATATGGCGAACGATTTGCCCAAAATGATGGACATGTTTCCGGCATGAGGGGACGGGGCCCTACTTGGAAATCTGACATGTCTGAGGTGTGGGATTTTCCAACATATGGTGGTTCCAGAGGTGGACATATTGGTTCGAGGAAGGCTGTAGGAGGTGGTCCTGGGGATGGTAGGTCGCCCAAATCAGAGAATGGGAGTGATCAAGGTAACAGCAGGCGAGCATGGGATAAAGGGGCTGGACCTGTTAGGTTTGGTGAGGGGCCTTCCGCAAGAAGTGTTTGGCAAGCTTCCAAAGATGAAGCTACCTTGGAAGCAATTCGTGTGGCTGGAGAGGACAATGGAACATCTAGATCTGGAAGAGTAGCTATACCTGAATTGACTGCAGAGGCTATGGAAGATGATAATGTGGGCCAGGAGCGGAATCCAATCTGGAATTCTTGGACTAATGCAATGGATGCTCTTCAAGTGGGTGATCTGGATACGGCCTTTTTGGAAGTGTTGTCTACAGGAGATGATATCTTGCTTGTAAAGTTGATGGACAGATCAGGTCCTGTGATTGACCAACTTTCAAATGAGACAGCAACCGAGGTCTTGCATGCTGTTGGGCAATTCTTACCAGAGCCAAACTTGTTTGACATCTGTTTGGCTTGGATTCAGCAGTTGGTTGAAATAGTGCTCGAAAATGGATCTGATGTTTTCGGTCTTCCCACTGACGTAAAGAAAGAACTCGTTTTGAATCTGCATGAAGCTTCTGTAACACTGAATCTGCCCGAGGACTGGGAAGGTGCAACACCGGATCAACTTTTGGTGCAGTTGGCATCATCCTGGGGAATCAATCCTCAGCAGCATGACAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009653 GO:0009826 GO:0009987 GO:0010005 GO:0010031 GO:0015630 GO:0015631 GO:0016043 GO:0016049 GO:0030863 GO:0030981 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050879 GO:0055028 GO:0060560 GO:0071840 GO:0071944 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

939

Amino Acids

101.89

Weight (kDa)

5.84

Isoelectric Point (pI)

37.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOR1L1_N	PF24714	51 - 329	1.6e-122	TORTIFOLIA1 N-terminal
TOR1L1_C	PF24713	796 - 929	2.8e-64	TORTIFOLIA1 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27060
fragaria_vesca	FvH4_3g22240
malus_domestica	MD03G1206600.v1.1 MD11G1222000.v1.1
prunus_persica	Prupe.4G209400_v2.0.a1 Prupe.4G209400_v2.0.a1
pyrus_communis	pycom03g15640 pycom11g19580
rosa_chinensis	RchiOBHm_Chr5g0038291
rosa_laevigata	RLG00000033844
rosa_multiflora	Rmu_sc0000764.1_g000003 Rmu_sc0003858.1_g000004 Rmu_sc0035524.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042730
rosa_rugosa	Rorug05G0171800
rosa_samantha	Rh5AG259100 Rh5BG262900 Rh5CG295300 Rh5DG271900
rosa_wichuraiana	Rw5G024250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1028, 1707
Acc36I	ACCTGC	1 cut(s)	1595
AccB7I	CCANNNNNTGG	2 cut(s)	769, 2066
AccBSI	CCGCTC	1 cut(s)	2377
AccI	GTMKAC	4 cut(s)	618, 1300, 1463, 2462
AccII	CGCG	3 cut(s)	452, 459, 473
AclWI	GGATC	7 cut(s)	299, 677, 1420, 1632, 1645, 2653, 2763
AcoI	YGGCCR	3 cut(s)	144, 561, 1505
AcsI	RAATTY	6 cut(s)	21, 1072, 1235, 1357, 1956, 2392
AcuI	CTGAAG	2 cut(s)	1122, 1653
AcyI	GRCGYC	3 cut(s)	294, 441, 462
AfaI	GTAC	2 cut(s)	209, 488
AflIII	ACRYGT	6 cut(s)	204, 450, 824, 1303, 1993, 2037
AhdI	GACNNNNNGTC	1 cut(s)	517
AjnI	CCWGG	4 cut(s)	141, 2119, 2370, 2783
AjuI	GAANNNNNNNTTGG	4 cut(s)	79, 111, 2377, 2409
Alw21I	GWGCWC	3 cut(s)	159, 812, 2637
Alw26I	GTCTC	8 cut(s)	195, 358, 744, 1036, 1041, 1693, 1752, 2528
AlwI	GGATC	7 cut(s)	299, 677, 1420, 1632, 1645, 2653, 2763
AlwNI	CAGNNNCTG	3 cut(s)	839, 1145, 2510
Ama87I	CYCGRG	3 cut(s)	230, 914, 2729
AoxI	GGCC	9 cut(s)	144, 262, 561, 1505, 1886, 2017, 2219, 2368, 2442
ApaI	GGGCCC	1 cut(s)	2021
ApeKI	GCWGC	6 cut(s)	70, 76, 833, 836, 1690, 2803
ApoI	RAATTY	6 cut(s)	21, 1072, 1235, 1357, 1956, 2392
Asp700I	GAANNNNTTC	2 cut(s)	895, 2274
AspLEI	GCGC	6 cut(s)	339, 473, 475, 484, 626, 760
AspS9I	GGNCC	9 cut(s)	14, 262, 2017, 2018, 2117, 2198, 2219, 2368, 2507
AsuC2I	CCSGG	1 cut(s)	446
AsuHPI	GGTGA	5 cut(s)	45, 166, 367, 2225, 2441
AvaI	CYCGRG	3 cut(s)	230, 914, 2729
AvaII	GGWCC	4 cut(s)	14, 2117, 2198, 2507
BaeGI	GKGCMC	1 cut(s)	2021
BalI	TGGCCA	2 cut(s)	146, 1507
BamHI	GGATCC	1 cut(s)	1637
BanII	GRGCYC	2 cut(s)	159, 2021
BauI	CACGAG	1 cut(s)	1815
BbsI	GAAGAC	3 cut(s)	1812, 1869, 2654
Bbv12I	GWGCWC	3 cut(s)	159, 812, 2637
BbvCI	CCTCAGC	1 cut(s)	2799
BbvI	GCAGC	5 cut(s)	82, 88, 845, 848, 1702
BccI	CCATC	8 cut(s)	58, 1214, 1616, 1855, 1943, 1982, 2120, 2488
BceAI	ACGGC	5 cut(s)	203, 302, 909, 1921, 2457
BcgI	CGANNNNNNTGC	4 cut(s)	849, 883, 1958, 1992
BciT130I	CCWGG	4 cut(s)	143, 2121, 2372, 2785
BciVI	GTATCC	1 cut(s)	2431
BclI	TGATCA	1 cut(s)	2158
BcnI	CCSGG	1 cut(s)	446
BcoDI	GTCTC	8 cut(s)	195, 358, 744, 1036, 1041, 1693, 1752, 2528
BfaI	CTAG	2 cut(s)	1083, 2307
BfmI	CTRYAG	3 cut(s)	2106, 2340, 2463
BfuAI	ACCTGC	1 cut(s)	1595
BfuI	GTATCC	1 cut(s)	2431
BglII	AGATCT	2 cut(s)	226, 2309
BlpI	GCTNAGC	1 cut(s)	36
BmcAI	AGTACT	1 cut(s)	488
Bme1390I	CCNGG	5 cut(s)	143, 446, 2121, 2372, 2785
Bme18I	GGWCC	4 cut(s)	14, 2117, 2198, 2507
BmeRI	GACNNNNNGTC	1 cut(s)	517
BmeT110I	CYCGRG	3 cut(s)	230, 914, 2729
BmgT120I	GGNCC	9 cut(s)	14, 262, 2017, 2018, 2117, 2198, 2219, 2368, 2507
BmiI	GGNNCC	6 cut(s)	16, 1639, 2018, 2019, 2074, 2220
BmrFI	CCNGG	5 cut(s)	143, 446, 2121, 2372, 2785
BmrI	ACTGGG	1 cut(s)	2746
BmsI	GCATC	4 cut(s)	418, 719, 2404, 2786
BmuI	ACTGGG	1 cut(s)	2746
BpiI	GAAGAC	3 cut(s)	1812, 1869, 2654
BplI	GAGNNNNNCTC	2 cut(s)	724, 756
BpmI	CTGGAG	3 cut(s)	1519, 1715, 2307
Bpu10I	CCTNAGC	2 cut(s)	705, 2799
Bpu1102I	GCTNAGC	1 cut(s)	36
BpuEI	CTTGAG	3 cut(s)	143, 407, 1811
BpuMI	CCSGG	1 cut(s)	446
BsaAI	YACGTR	2 cut(s)	207, 827
BsaBI	GATNNNNATC	1 cut(s)	1575
BsaHI	GRCGYC	3 cut(s)	294, 441, 462
BsaI	GGTCTC	2 cut(s)	358, 1752
BsaJI	CCNNGG	8 cut(s)	147, 665, 802, 2120, 2265, 2544, 2730, 2784
BsaWI	WCCGGW	2 cut(s)	1377, 2752
BsaXI	ACNNNNNCTCC	2 cut(s)	281, 311
Bse118I	RCCGGY	2 cut(s)	31, 721
Bse1I	ACTGG	4 cut(s)	1164, 1593, 1927, 2741
Bse3DI	GCAATG	4 cut(s)	399, 860, 968, 2415
Bse8I	GATNNNNATC	1 cut(s)	1575
BseBI	CCWGG	4 cut(s)	143, 2121, 2372, 2785
BseDI	CCNNGG	8 cut(s)	147, 665, 802, 2120, 2265, 2544, 2730, 2784
BseGI	GGATG	5 cut(s)	240, 433, 2131, 2419, 2780
BseJI	GATNNNNATC	1 cut(s)	1575
BseMI	GCAATG	4 cut(s)	399, 860, 968, 2415
BseMII	CTCAG	4 cut(s)	755, 1035, 2034, 2813
BseNI	ACTGG	4 cut(s)	1164, 1593, 1927, 2741
BsePI	GCGCGC	1 cut(s)	471
BseRI	GAGGAG	2 cut(s)	82, 266
BseSI	GKGCMC	1 cut(s)	2021
BseXI	GCAGC	5 cut(s)	82, 88, 845, 848, 1702
BseYI	CCCAGC	1 cut(s)	992
BsgI	GTGCAG	2 cut(s)	1623, 2789
Bsh1236I	CGCG	3 cut(s)	452, 459, 473
Bsh1285I	CGRYCG	1 cut(s)	199
BshFI	GGCC	9 cut(s)	146, 264, 563, 1507, 1888, 2019, 2221, 2370, 2444
BsiEI	CGRYCG	1 cut(s)	199
BsiHKAI	GWGCWC	3 cut(s)	159, 812, 2637
BsiHKCI	CYCGRG	3 cut(s)	230, 914, 2729
BsiSI	CCGG	9 cut(s)	12, 32, 257, 438, 445, 722, 1378, 2001, 2753
BslFI	GGGAC	2 cut(s)	461, 2025
BsmAI	GTCTC	8 cut(s)	195, 358, 744, 1036, 1041, 1693, 1752, 2528
BsmBI	CGTCTC	1 cut(s)	1041
BsmFI	GGGAC	2 cut(s)	461, 2025
BsmI	GAATGC	2 cut(s)	803, 865
BsnI	GGCC	9 cut(s)	146, 264, 563, 1507, 1888, 2019, 2221, 2370, 2444
Bso31I	GGTCTC	2 cut(s)	358, 1752
BsoBI	CYCGRG	3 cut(s)	230, 914, 2729
Bsp120I	GGGCCC	1 cut(s)	2017
Bsp1286I	GDGCHC	4 cut(s)	159, 812, 2021, 2637
Bsp1720I	GCTNAGC	1 cut(s)	36
Bsp19I	CCATGG	1 cut(s)	147
BspANI	GGCC	9 cut(s)	146, 264, 563, 1507, 1888, 2019, 2221, 2370, 2444
BspCNI	CTCAG	4 cut(s)	754, 1036, 2035, 2812
BspFNI	CGCG	3 cut(s)	452, 459, 473
BspHI	TCATGA	3 cut(s)	793, 1480, 1750
BspLI	GGNNCC	6 cut(s)	16, 1639, 2018, 2019, 2074, 2220
BspMAI	CTGCAG	1 cut(s)	2344
BspMI	ACCTGC	1 cut(s)	1595
BspPI	GGATC	7 cut(s)	299, 677, 1420, 1632, 1645, 2653, 2763
BspQI	GCTCTTC	1 cut(s)	2424
BspTNI	GGTCTC	2 cut(s)	358, 1752
BsrBI	CCGCTC	1 cut(s)	2377
BsrDI	GCAATG	4 cut(s)	399, 860, 968, 2415
BsrFI	RCCGGY	2 cut(s)	31, 721
BsrI	ACTGG	4 cut(s)	1164, 1593, 1927, 2741
BssAI	RCCGGY	2 cut(s)	31, 721
BssECI	CCNNGG	8 cut(s)	147, 665, 802, 2120, 2265, 2544, 2730, 2784
BssHII	GCGCGC	1 cut(s)	471
BssNAI	GTATAC	1 cut(s)	1464
BssNI	GRCGYC	3 cut(s)	294, 441, 462
BssSI	CACGAG	1 cut(s)	1815
BssT1I	CCWWGG	4 cut(s)	147, 665, 802, 2265
Bst1107I	GTATAC	1 cut(s)	1464
Bst2BI	CACGAG	1 cut(s)	1815
Bst2UI	CCWGG	4 cut(s)	143, 2121, 2372, 2785
Bst4CI	ACNGT	3 cut(s)	600, 910, 1433
Bst6I	CTCTTC	5 cut(s)	100, 1837, 1933, 2311, 2424
BstACI	GRCGYC	3 cut(s)	294, 441, 462
BstAPI	GCANNNNNTGC	2 cut(s)	1687, 2414
BstBAI	YACGTR	2 cut(s)	207, 827
BstC8I	GCNNGC	8 cut(s)	341, 345, 454, 473, 2174, 2178, 2244, 2556
BstDEI	CTNAG	7 cut(s)	36, 609, 705, 741, 1044, 2043, 2799
BstDSI	CCRYGG	1 cut(s)	147
BstENI	CCTNNNNNAGG	1 cut(s)	740
BstF5I	GGATG	5 cut(s)	240, 433, 2131, 2419, 2780
BstFNI	CGCG	3 cut(s)	452, 459, 473
BstHHI	GCGC	6 cut(s)	339, 473, 475, 484, 626, 760
BstMAI	GTCTC	8 cut(s)	195, 358, 744, 1036, 1041, 1693, 1752, 2528
BstMCI	CGRYCG	1 cut(s)	199
BstNI	CCWGG	4 cut(s)	143, 2121, 2372, 2785
BstNSI	RCATGY	5 cut(s)	1307, 1726, 1997, 2041, 2558
BstSCI	CCNGG	5 cut(s)	141, 444, 2119, 2370, 2783
BstSFI	CTRYAG	3 cut(s)	2106, 2340, 2463
BstSLI	GKGCMC	1 cut(s)	2021
BstUI	CGCG	3 cut(s)	452, 459, 473
BstV1I	GCAGC	5 cut(s)	82, 88, 845, 848, 1702
BstV2I	GAAGAC	3 cut(s)	1812, 1869, 2654
BstX2I	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2309, 2645
BstXI	CCANNNNNNTGG	1 cut(s)	1988
BstYI	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2309, 2645
BstZ17I	GTATAC	1 cut(s)	1464
BsuI	GTATCC	1 cut(s)	2431
BsuRI	GGCC	9 cut(s)	146, 264, 563, 1507, 1888, 2019, 2221, 2370, 2444
BtgI	CCRYGG	1 cut(s)	147
BtgZI	GCGATG	1 cut(s)	696
BtsCI	GGATG	5 cut(s)	240, 433, 2131, 2419, 2780
BtsIMutI	CAGTG	5 cut(s)	596, 1171, 1920, 2667, 2717
BveI	ACCTGC	1 cut(s)	1595
Cac8I	GCNNGC	8 cut(s)	341, 345, 454, 473, 2174, 2178, 2244, 2556
CaiI	CAGNNNCTG	3 cut(s)	839, 1145, 2510
CciI	TCATGA	3 cut(s)	793, 1480, 1750
CfoI	GCGC	6 cut(s)	339, 473, 475, 484, 626, 760
Cfr10I	RCCGGY	2 cut(s)	31, 721
Cfr13I	GGNCC	9 cut(s)	14, 262, 2017, 2018, 2117, 2198, 2219, 2368, 2507
CseI	GACGC	4 cut(s)	302, 430, 458, 470
Csp6I	GTAC	2 cut(s)	208, 487
CspCI	CAANNNNNGTGG	4 cut(s)	1147, 1182, 2262, 2297
CviQI	GTAC	2 cut(s)	208, 487
DdeI	CTNAG	7 cut(s)	36, 609, 705, 741, 1044, 2043, 2799
DrdI	GACNNNNNNGTC	2 cut(s)	1028, 1707
DriI	GACNNNNNGTC	1 cut(s)	517
DseDI	GACNNNNNNGTC	2 cut(s)	1028, 1707
EaeI	YGGCCR	3 cut(s)	144, 561, 1505
Eam1104I	CTCTTC	5 cut(s)	100, 1837, 1933, 2311, 2424
Eam1105I	GACNNNNNGTC	1 cut(s)	517
EarI	CTCTTC	5 cut(s)	100, 1837, 1933, 2311, 2424
EciI	GGCGGA	1 cut(s)	289
Ecl136II	GAGCTC	1 cut(s)	157
Eco130I	CCWWGG	4 cut(s)	147, 665, 802, 2265
Eco147I	AGGCCT	1 cut(s)	1888
Eco24I	GRGCYC	2 cut(s)	159, 2021
Eco31I	GGTCTC	2 cut(s)	358, 1752
Eco32I	GATATC	1 cut(s)	2476
Eco47I	GGWCC	4 cut(s)	14, 2117, 2198, 2507
Eco53kI	GAGCTC	1 cut(s)	157
Eco57I	CTGAAG	2 cut(s)	1122, 1653
Eco88I	CYCGRG	3 cut(s)	230, 914, 2729
EcoICRI	GAGCTC	1 cut(s)	157
EcoNI	CCTNNNNNAGG	1 cut(s)	740
EcoO109I	RGGNCCY	5 cut(s)	262, 2017, 2018, 2219, 2507
EcoRI	GAATTC	3 cut(s)	1235, 1956, 2392
EcoRII	CCWGG	4 cut(s)	141, 2119, 2370, 2783
EcoRV	GATATC	1 cut(s)	2476
EcoT14I	CCWWGG	4 cut(s)	147, 665, 802, 2265
EcoT38I	GRGCYC	2 cut(s)	159, 2021
ErhI	CCWWGG	4 cut(s)	147, 665, 802, 2265
Esp3I	CGTCTC	1 cut(s)	1041
FalI	AAGNNNNNCTT	2 cut(s)	1698, 1730
FaqI	GGGAC	2 cut(s)	461, 2025
FauI	CCCGC	5 cut(s)	261, 322, 642, 1171, 1736
FauNDI	CATATG	2 cut(s)	1963, 2065
FbaI	TGATCA	1 cut(s)	2158
FblI	GTMKAC	4 cut(s)	618, 1300, 1463, 2462
FokI	GGATG	5 cut(s)	227, 440, 2138, 2426, 2767
FriOI	GRGCYC	2 cut(s)	159, 2021
FspBI	CTAG	2 cut(s)	1083, 2307
GlaI	GCGC	6 cut(s)	338, 472, 474, 483, 625, 759
GsaI	CCCAGC	1 cut(s)	996
GsuI	CTGGAG	3 cut(s)	1519, 1715, 2307
HaeIII	GGCC	9 cut(s)	146, 264, 563, 1507, 1888, 2019, 2221, 2370, 2444
HapII	CCGG	9 cut(s)	12, 32, 257, 438, 445, 722, 1378, 2001, 2753
HgaI	GACGC	4 cut(s)	302, 430, 458, 470
HhaI	GCGC	6 cut(s)	339, 473, 475, 484, 626, 760
Hin1I	GRCGYC	3 cut(s)	294, 441, 462
Hin6I	GCGC	6 cut(s)	337, 471, 473, 482, 624, 758
HinP1I	GCGC	6 cut(s)	337, 471, 473, 482, 624, 758
HincII	GTYRAC	1 cut(s)	1628
HindII	GTYRAC	1 cut(s)	1628
HindIII	AAGCTT	3 cut(s)	766, 2244, 2706
HpaII	CCGG	9 cut(s)	12, 32, 257, 438, 445, 722, 1378, 2001, 2753
HphI	GGTGA	5 cut(s)	45, 166, 367, 2225, 2441
Hpy166II	GTNNAC	7 cut(s)	537, 619, 1301, 1464, 1628, 2084, 2463
Hpy8I	GTNNAC	7 cut(s)	537, 619, 1301, 1464, 1628, 2084, 2463
Hpy99I	CGWCG	2 cut(s)	296, 464
HpyAV	CCTTC	5 cut(s)	487, 1628, 2095, 2232, 2735
HpyCH4III	ACNGT	3 cut(s)	600, 910, 1433
HpyCH4IV	ACGT	3 cut(s)	206, 826, 2673
HpyF3I	CTNAG	7 cut(s)	36, 609, 705, 741, 1044, 2043, 2799
HpySE526I	ACGT	3 cut(s)	206, 826, 2673
Hsp92I	GRCGYC	3 cut(s)	294, 441, 462
HspAI	GCGC	6 cut(s)	337, 471, 473, 482, 624, 758
Ksp22I	TGATCA	1 cut(s)	2158
LguI	GCTCTTC	1 cut(s)	2424
LmnI	GCTCC	7 cut(s)	154, 279, 749, 807, 1018, 1103, 2374
Lsp1109I	GCAGC	5 cut(s)	82, 88, 845, 848, 1702
LweI	GCATC	4 cut(s)	418, 719, 2404, 2786
MaeI	CTAG	2 cut(s)	1083, 2307
MaeII	ACGT	3 cut(s)	206, 826, 2673
MaeIII	GTNAC	4 cut(s)	1433, 1925, 2165, 2713
MbiI	CCGCTC	1 cut(s)	2377
MfeI	CAATTG	1 cut(s)	1682
MflI	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2309, 2645
MhlI	GDGCHC	4 cut(s)	159, 812, 2021, 2637
MlsI	TGGCCA	2 cut(s)	146, 1507
MluI	ACGCGT	1 cut(s)	450
MluNI	TGGCCA	2 cut(s)	146, 1507
MlyI	GAGTC	4 cut(s)	338, 926, 1526, 1595
MmeI	TCCRAC	3 cut(s)	219, 727, 2084
Mox20I	TGGCCA	2 cut(s)	146, 1507
MroXI	GAANNNNTTC	2 cut(s)	895, 2274
MscI	TGGCCA	2 cut(s)	146, 1507
MseI	TTAA	4 cut(s)	684, 939, 1211, 1398
Msp20I	TGGCCA	2 cut(s)	146, 1507
MspA1I	CMGCKG	2 cut(s)	839, 1693
MspI	CCGG	9 cut(s)	12, 32, 257, 438, 445, 722, 1378, 2001, 2753
MspR9I	CCNGG	5 cut(s)	143, 446, 2121, 2372, 2785
MunI	CAATTG	1 cut(s)	1682
Mva1269I	GAATGC	2 cut(s)	803, 865
MvaI	CCWGG	4 cut(s)	143, 2121, 2372, 2785
MvnI	CGCG	3 cut(s)	452, 459, 473
NciI	CCSGG	1 cut(s)	446
NcoI	CCATGG	1 cut(s)	147
NdeI	CATATG	2 cut(s)	1963, 2065
NlaIV	GGNNCC	6 cut(s)	16, 1639, 2018, 2019, 2074, 2220
NmeAIII	GCCGAG	1 cut(s)	589
NspI	RCATGY	5 cut(s)	1307, 1726, 1997, 2041, 2558
PaeI	GCATGC	1 cut(s)	2558
PaeR7I	CTCGAG	2 cut(s)	230, 914
PagI	TCATGA	3 cut(s)	793, 1480, 1750
PauI	GCGCGC	1 cut(s)	471
PceI	AGGCCT	1 cut(s)	1888
PciI	ACATGT	3 cut(s)	1303, 1993, 2037
PciSI	GCTCTTC	1 cut(s)	2424
PcsI	WCGNNNNNNNCGW	2 cut(s)	300, 456
PctI	GAATGC	2 cut(s)	803, 865
PdmI	GAANNNNTTC	2 cut(s)	895, 2274
PfeI	GAWTC	8 cut(s)	168, 434, 1142, 2380, 2611, 2695, 2722, 2790
PflMI	CCANNNNNTGG	2 cut(s)	769, 2066
PfoI	TCCNGGA	1 cut(s)	444
PleI	GAGTC	4 cut(s)	337, 925, 1526, 1594
PpsI	GAGTC	4 cut(s)	337, 925, 1526, 1594
Ppu21I	YACGTR	2 cut(s)	207, 827
PpuMI	RGGWCCY	1 cut(s)	2507
PscI	ACATGT	3 cut(s)	1303, 1993, 2037
Psp124BI	GAGCTC	1 cut(s)	159
Psp5II	RGGWCCY	1 cut(s)	2507
Psp6I	CCWGG	4 cut(s)	141, 2119, 2370, 2783
PspFI	CCCAGC	1 cut(s)	992
PspGI	CCWGG	4 cut(s)	141, 2119, 2370, 2783
PspN4I	GGNNCC	6 cut(s)	16, 1639, 2018, 2019, 2074, 2220
PspOMI	GGGCCC	1 cut(s)	2017
PspPI	GGNCC	9 cut(s)	14, 262, 2017, 2018, 2117, 2198, 2219, 2368, 2507
PspPPI	RGGWCCY	1 cut(s)	2507
PspXI	VCTCGAGB	1 cut(s)	914
PstI	CTGCAG	1 cut(s)	2344
PstNI	CAGNNNCTG	3 cut(s)	839, 1145, 2510
PsuI	RGATCY	6 cut(s)	226, 669, 1412, 1637, 2309, 2645
PteI	GCGCGC	1 cut(s)	471
PvuII	CAGCTG	2 cut(s)	839, 1693
RsaI	GTAC	2 cut(s)	209, 488
RsaNI	GTAC	2 cut(s)	208, 487
SacI	GAGCTC	1 cut(s)	159
SapI	GCTCTTC	1 cut(s)	2424
SaqAI	TTAA	4 cut(s)	684, 939, 1211, 1398
Sau96I	GGNCC	9 cut(s)	14, 262, 2017, 2018, 2117, 2198, 2219, 2368, 2507
ScaI	AGTACT	1 cut(s)	488
SchI	GAGTC	4 cut(s)	338, 926, 1526, 1595
ScrFI	CCNGG	5 cut(s)	143, 446, 2121, 2372, 2785
SduI	GDGCHC	4 cut(s)	159, 812, 2021, 2637
SfaNI	GCATC	4 cut(s)	418, 719, 2404, 2786
SfcI	CTRYAG	3 cut(s)	2106, 2340, 2463
Sfr274I	CTCGAG	2 cut(s)	230, 914
SinI	GGWCC	4 cut(s)	14, 2117, 2198, 2507
SlaI	CTCGAG	2 cut(s)	230, 914
SmlI	CTYRAG	5 cut(s)	158, 230, 422, 914, 1790
SmoI	CTYRAG	5 cut(s)	158, 230, 422, 914, 1790
SphI	GCATGC	1 cut(s)	2558
SseBI	AGGCCT	1 cut(s)	1888
SspMI	CTAG	2 cut(s)	1083, 2307
SstI	GAGCTC	1 cut(s)	159
StuI	AGGCCT	1 cut(s)	1888
StyD4I	CCNGG	5 cut(s)	141, 444, 2119, 2370, 2783
StyI	CCWWGG	4 cut(s)	147, 665, 802, 2265
TaaI	ACNGT	3 cut(s)	600, 910, 1433
TaiI	ACGT	3 cut(s)	209, 829, 2676
TaqI	TCGA	8 cut(s)	134, 231, 731, 869, 915, 1859, 2096, 2637
TaqII	GACCGA	2 cut(s)	213, 2648
TatI	WGTACW	1 cut(s)	486
TauI	GCSGC	5 cut(s)	67, 70, 76, 459, 596
TfiI	GAWTC	8 cut(s)	168, 434, 1142, 2380, 2611, 2695, 2722, 2790
Tru1I	TTAA	4 cut(s)	684, 939, 1211, 1398
Tru9I	TTAA	4 cut(s)	684, 939, 1211, 1398
TscAI	CASTG	5 cut(s)	603, 1171, 1927, 2674, 2724
TseI	GCWGC	6 cut(s)	70, 76, 833, 836, 1690, 2803
TspDTI	ATGAA	9 cut(s)	782, 810, 1023, 1104, 1116, 1397, 1726, 2271, 2718
TspRI	CASTG	5 cut(s)	603, 1171, 1927, 2674, 2724
Van91I	CCANNNNNTGG	2 cut(s)	769, 2066
VpaK11BI	GGWCC	4 cut(s)	14, 2117, 2198, 2507
XagI	CCTNNNNNAGG	1 cut(s)	740
XapI	RAATTY	6 cut(s)	21, 1072, 1235, 1357, 1956, 2392
XbaI	TCTAGA	1 cut(s)	2306
XceI	RCATGY	5 cut(s)	1307, 1726, 1997, 2041, 2558
XhoI	CTCGAG	2 cut(s)	230, 914
XmiI	GTMKAC	4 cut(s)	618, 1300, 1463, 2462
XmnI	GAANNNNTTC	2 cut(s)	895, 2274
XspI	CTAG	2 cut(s)	1083, 2307
ZrmI	AGTACT	1 cut(s)	488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.