MD03G1209200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013914
Physical Location & Seq
Forward (+)
28869191 .. 28870425
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1209200.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGAAGATCGCTCCAGAAACTCTATGATGGGAAGAGGTAATTCTTCTGATACAAGAACCACTACTCAAGAGTCCCCTGAGGAGAGTAGTTGGACTATGTACTTGCAAGATTTCATGATAACCAATAGTCAAGACGATGACGATGACGATGACGACCGCAGTTCTTTCTCCTCATACGGTTATGAGAATTGTTCCATAATCTCCGACGCCGCTTCTTCAGTCACCCGAAAGTTTACTATTAATGGAGAAGTTTTAGGGTTTGGCGTGGTTGGAGGTTATAATAGATCTGACGTCATGATTAAGAGAAAACCCAAGGAAGCTTTTCTTGATGATGCTTTGGAGGACACAGCTAGTTCTCCAGTAAATAGTCCCAAGGTTTGTAATCTAAGCCAGTCAACTGTGGAACCAACAATCCAAAAAGATTATAAAGACATACCTAATGCGGAGAAAGGAAGTGCTTCTGGCCGGGTGGATGAGAGAAATGAATCGGGATTTATTGGGAGAGAGAGTGACTGCACAGAATTGAAGAAAAGGGGACTTTGCTTGGTTCCATTTTCTATGGTAGCAAACTATTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

21.37

Weight (kDa)

4.5

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013914)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54790
fragaria_vesca FvH4_3g22010 FvH4_3g22010
malus_domestica MD03G1209200.v1.1 MD11G1224000.v1.1
prunus_persica Prupe.4G205800_v2.0.a1 Prupe.4G205800_v2.0.a1
pyrus_communis pycom03g15810 pycom11g19760
rosa_laevigata RLG00000033818
rosa_roxburghii Rroxscaffold_1G00043060
rosa_rugosa Rorug05G0168200 Rorug05G0168200
rosa_samantha Rh5BG259500 Rh5CG292500 Rh5DG269400
rosa_wichuraiana Rw5G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 279, 426
AatII GACGTC 1 cut(s) 294
AciI CCGC 3 cut(s) 157, 210, 443
AcoI YGGCCR 1 cut(s) 463
AcuI CTGAAG 1 cut(s) 201
AcyI GRCGYC 2 cut(s) 207, 291
AfaI GTAC 1 cut(s) 101
AgsI TTSAA 1 cut(s) 526
AloI GAACNNNNNNTCC 2 cut(s) 396, 428
AluBI AGCT 2 cut(s) 320, 350
AluI AGCT 2 cut(s) 320, 350
AoxI GGCC 1 cut(s) 463
AseI ATTAAT 1 cut(s) 240
Asp700I GAANNNNTTC 2 cut(s) 321, 457
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 214
AxyI CCTNAGG 1 cut(s) 78
BccI CCATC 1 cut(s) 22
BcnI CCSGG 1 cut(s) 467
BfaI CTAG 1 cut(s) 351
BglII AGATCT 1 cut(s) 284
BisI GCNGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 211
Bme1390I CCNGG 1 cut(s) 467
BmiI GGNNCC 2 cut(s) 405, 549
BmrFI CCNGG 1 cut(s) 467
BmsI GCATC 1 cut(s) 322
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 467
BsaHI GRCGYC 2 cut(s) 207, 291
BsaJI CCNNGG 2 cut(s) 312, 372
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bse1I ACTGG 2 cut(s) 359, 391
Bse21I CCTNAGG 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 312, 372
BseGI GGATG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 2 cut(s) 359, 391
BseRI GAGGAG 2 cut(s) 95, 160
BsgI GTGCAG 1 cut(s) 499
Bsh1285I CGRYCG 1 cut(s) 157
BshFI GGCC 1 cut(s) 465
BsiEI CGRYCG 1 cut(s) 157
BsiSI CCGG 1 cut(s) 466
BslFI GGGAC 3 cut(s) 58, 354, 549
BsmFI GGGAC 3 cut(s) 58, 354, 549
BsnI GGCC 1 cut(s) 465
Bsp143I GATC 2 cut(s) 7, 284
BspACI CCGC 3 cut(s) 157, 210, 443
BspANI GGCC 1 cut(s) 465
BspCNI CTCAG 1 cut(s) 70
BspHI TCATGA 2 cut(s) 114, 294
BspLI GGNNCC 2 cut(s) 405, 549
BsrI ACTGG 2 cut(s) 359, 391
BssECI CCNNGG 2 cut(s) 312, 372
BssMI GATC 2 cut(s) 7, 284
BssNI GRCGYC 2 cut(s) 207, 291
BssT1I CCWWGG 2 cut(s) 312, 372
Bst4CI ACNGT 2 cut(s) 179, 400
Bst6I CTCTTC 1 cut(s) 28
BstACI GRCGYC 2 cut(s) 207, 291
BstDEI CTNAG 2 cut(s) 78, 386
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 2 cut(s) 10, 287
BstMBI GATC 2 cut(s) 7, 284
BstMCI CGRYCG 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
Bsu36I CCTNAGG 1 cut(s) 78
BsuRI GGCC 1 cut(s) 465
BtsCI GGATG 1 cut(s) 478
CciI TCATGA 2 cut(s) 114, 294
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 115, 295
CviJI RGCY 4 cut(s) 320, 350, 390, 465
CviKI_1 RGCY 4 cut(s) 320, 350, 390, 465
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 78, 386
DpnI GATC 2 cut(s) 9, 286
DpnII GATC 2 cut(s) 7, 284
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 312, 372
Eco57I CTGAAG 1 cut(s) 201
Eco81I CCTNAGG 1 cut(s) 78
EcoT14I CCWWGG 2 cut(s) 312, 372
ErhI CCWWGG 2 cut(s) 312, 372
FaeI CATG 2 cut(s) 118, 298
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 3 cut(s) 58, 354, 549
FatI CATG 2 cut(s) 114, 294
Fnu4HI GCNGC 1 cut(s) 210
FokI GGATG 1 cut(s) 485
Fsp4HI GCNGC 1 cut(s) 210
FspBI CTAG 1 cut(s) 351
GluI GCNGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 465
HapII CCGG 1 cut(s) 466
HgaI GACGC 1 cut(s) 215
Hin1I GRCGYC 2 cut(s) 207, 291
Hin1II CATG 2 cut(s) 118, 298
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 2 cut(s) 71, 485
HpaII CCGG 1 cut(s) 466
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 2 cut(s) 234, 396
Hpy188I TCNGA 3 cut(s) 49, 205, 289
Hpy188III TCNNGA 7 cut(s) 15, 68, 115, 131, 295, 326, 489
Hpy8I GTNNAC 2 cut(s) 234, 396
Hpy99I CGWCG 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 179, 400
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 2 cut(s) 106, 516
HpyF3I CTNAG 2 cut(s) 78, 386
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 2 cut(s) 207, 291
Hsp92II CATG 2 cut(s) 118, 298
Kzo9I GATC 2 cut(s) 7, 284
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 6 cut(s) 28, 90, 372, 404, 447, 479
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 220, 509
MalI GATC 2 cut(s) 9, 286
MboI GATC 2 cut(s) 7, 284
MboII GAAGA 5 cut(s) 17, 36, 45, 207, 538
MflI RGATCY 1 cut(s) 284
MluCI AATT 3 cut(s) 40, 187, 521
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 3 cut(s) 71, 228, 250
MnlI CCTC 5 cut(s) 29, 73, 181, 266, 334
MroXI GAANNNNTTC 2 cut(s) 321, 457
MseI TTAA 2 cut(s) 240, 300
MspI CCGG 1 cut(s) 466
MspR9I CCNGG 1 cut(s) 467
NciI CCSGG 1 cut(s) 467
NdeII GATC 2 cut(s) 7, 284
NlaIII CATG 2 cut(s) 118, 298
NlaIV GGNNCC 2 cut(s) 405, 549
NmuCI GTSAC 2 cut(s) 220, 509
PagI TCATGA 2 cut(s) 114, 294
PdmI GAANNNNTTC 2 cut(s) 321, 457
PfeI GAWTC 1 cut(s) 485
PkrI GCNGC 1 cut(s) 211
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PshBI ATTAAT 1 cut(s) 240
PsiI TTATAA 2 cut(s) 279, 426
PspN4I GGNNCC 2 cut(s) 405, 549
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 2 cut(s) 240, 300
SatI GCNGC 1 cut(s) 210
Sau3AI GATC 2 cut(s) 7, 284
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 1 cut(s) 467
SetI ASST 7 cut(s) 40, 277, 294, 322, 352, 378, 439
SfaNI GCATC 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 3 cut(s) 40, 187, 521
SsiI CCGC 3 cut(s) 157, 210, 443
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 465
StyI CCWWGG 2 cut(s) 312, 372
TaaI ACNGT 2 cut(s) 179, 400
TaiI ACGT 1 cut(s) 294
TasI AATT 3 cut(s) 40, 187, 521
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 2 cut(s) 240, 300
Tru9I TTAA 2 cut(s) 240, 300
TseFI GTSAC 2 cut(s) 220, 509
Tsp45I GTSAC 2 cut(s) 220, 509
TspDTI ATGAA 2 cut(s) 103, 498
VspI ATTAAT 1 cut(s) 240
XmnI GAANNNNTTC 2 cut(s) 321, 457
XspI CTAG 1 cut(s) 351
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.