MD03G1212800.v1.1

psbQ-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014562
Physical Location & Seq
Forward (+)
29201001 .. 29202066
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1212800.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGCTCGCTTGGCAAACTTAAATGGAGTCTCCGAAACGCTGCCGGTCCTTCCAAGGTTTCCGAGCGTACAAACAACCCGAAAGAAAGCGAGCTTTATTTGCTTTACGGGCAAGAAAAGCAATGATTTTCAGGAACAGCCACTACAAACTACAAGAAGAATTGCATTGGGCCTGGCCTCTGTTGCACTTGTTGGCAACCCCTGCAATGGTGTCTCTCTTGCTGAGGGCAATGGGTTCTGGCTCGACGGCCCTCTGCCCGTGCCTTCGGCTCAAAACAAAATTGCAAATGAGAAGACGGGAACGCGGTCTTTTCTGAAGAGGGGACTCTACATGGCAAATATCGGAACCAAAGGGAGGAAGTTCAGGCTTAAGAAGTACGCAAATGATCTCCTGGCAATGGCGGATTTGATTACGAAAGATACACTAAACTATGTCCGAAGGTATCTGCGGCTGAAATCCACATTCATGTACTTTGATTTCGACCAAGTTATCTCTGCAGCACCGGTCGACGAGAAACAACCACTCACAAATCTTGCTAACAGATTGTTTGACACCGTCGAAAAGCTTGATGGCGCCGTGAAGCAACGCGACCTCCCTCAGACAGAAACATACTACAAAGACACAACAGTTGTTCTCCAAGAGGTCATGGATCGAATGGCGTGA

Protein Analysis

221

Amino Acids

24.66

Weight (kDa)

9.72

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbQ PF05757 1 - 220 4.1e-53 Oxygen evolving enhancer protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014562)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01440
fragaria_vesca FvH4_3g21690
malus_domestica MD03G1212800.v1.1 MD11G1227900.v1.1
prunus_persica Prupe.4G201300_v2.0.a1
pyrus_communis pycom03g16160 pycom11g20060
rosa_chinensis RchiOBHm_Chr5g0037191
rosa_laevigata RLG00000033755
rosa_multiflora Rmu_co8022472.1_g000001
rosa_roxburghii Rroxscaffold_1G00043790
rosa_rugosa Rorug05G0161500
rosa_samantha Rh5AG253100 Rh5BG255000 Rh5CG287700 Rh5DG263500
rosa_wichuraiana Rw5G023380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 572
AccI GTMKAC 1 cut(s) 507
AccII CGCG 2 cut(s) 304, 588
AciI CCGC 3 cut(s) 304, 401, 448
AclWI GGATC 1 cut(s) 657
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 3 cut(s) 69, 377, 470
AfiI CCNNNNNNNGG 3 cut(s) 206, 354, 397
AflII CTTAAG 1 cut(s) 368
AgeI ACCGGT 1 cut(s) 502
AjnI CCWGG 2 cut(s) 171, 390
AluBI AGCT 2 cut(s) 93, 565
AluI AGCT 2 cut(s) 93, 565
Alw26I GTCTC 2 cut(s) 34, 217
AlwI GGATC 1 cut(s) 657
AoxI GGCC 3 cut(s) 169, 174, 247
ApeKI GCWGC 2 cut(s) 40, 497
AsiGI ACCGGT 1 cut(s) 502
AspLEI GCGC 1 cut(s) 575
AspS9I GGNCC 3 cut(s) 46, 169, 248
AvaII GGWCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 299
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 27, 509
BccI CCATC 1 cut(s) 563
BceAI ACGGC 2 cut(s) 262, 560
BciT130I CCWGG 2 cut(s) 173, 392
BcoDI GTCTC 2 cut(s) 34, 217
BfmI CTRYAG 1 cut(s) 495
BfoI RGCGCY 1 cut(s) 576
BfrI CTTAAG 1 cut(s) 368
BisI GCNGC 3 cut(s) 41, 449, 498
BlsI GCNGC 3 cut(s) 42, 450, 499
Bme1390I CCNGG 2 cut(s) 173, 392
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 46, 169, 248
BmiI GGNNCC 2 cut(s) 346, 574
BmrFI CCNGG 2 cut(s) 173, 392
BpiI GAAGAC 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 222
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 53
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 354, 397
Bse118I RCCGGY 2 cut(s) 43, 502
Bse3DI GCAATG 4 cut(s) 127, 211, 235, 402
BseBI CCWGG 2 cut(s) 173, 392
BseDI CCNNGG 1 cut(s) 53
BseLI CCNNNNNNNGG 3 cut(s) 206, 354, 397
BseMI GCAATG 4 cut(s) 127, 211, 235, 402
BseMII CTCAG 2 cut(s) 213, 611
BseXI GCAGC 2 cut(s) 27, 509
Bsh1236I CGCG 2 cut(s) 304, 588
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 3 cut(s) 171, 176, 249
BshNI GGYRCC 1 cut(s) 572
BshTI ACCGGT 1 cut(s) 502
BsiEI CGRYCG 1 cut(s) 507
BsiSI CCGG 2 cut(s) 44, 503
BslFI GGGAC 1 cut(s) 336
BslI CCNNNNNNNGG 3 cut(s) 206, 354, 397
BsmAI GTCTC 2 cut(s) 34, 217
BsmFI GGGAC 1 cut(s) 336
BsnI GGCC 3 cut(s) 171, 176, 249
Bsp143I GATC 2 cut(s) 385, 649
BspACI CCGC 3 cut(s) 304, 401, 448
BspANI GGCC 3 cut(s) 171, 176, 249
BspCNI CTCAG 2 cut(s) 214, 610
BspFNI CGCG 2 cut(s) 304, 588
BspLI GGNNCC 2 cut(s) 346, 574
BspMAI CTGCAG 1 cut(s) 499
BspPI GGATC 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 368
BsrDI GCAATG 4 cut(s) 127, 211, 235, 402
BsrFI RCCGGY 2 cut(s) 43, 502
BssAI RCCGGY 2 cut(s) 43, 502
BssECI CCNNGG 1 cut(s) 53
BssMI GATC 2 cut(s) 385, 649
BssNI GRCGYC 1 cut(s) 573
BssT1I CCWWGG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 173, 392
Bst4CI ACNGT 2 cut(s) 556, 628
Bst6I CTCTTC 1 cut(s) 311
BstACI GRCGYC 1 cut(s) 573
BstAFI CTTAAG 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 7, 91
BstDEI CTNAG 2 cut(s) 222, 597
BstFNI CGCG 2 cut(s) 304, 588
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 1 cut(s) 575
BstKTI GATC 2 cut(s) 388, 652
BstMAI GTCTC 2 cut(s) 34, 217
BstMBI GATC 2 cut(s) 385, 649
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 6 cut(s) 11, 99, 108, 117, 182, 201
BstNI CCWGG 2 cut(s) 173, 392
BstSCI CCNGG 2 cut(s) 171, 390
BstSFI CTRYAG 1 cut(s) 495
BstUI CGCG 2 cut(s) 304, 588
BstV1I GCAGC 2 cut(s) 27, 509
BstV2I GAAGAC 1 cut(s) 299
BsuRI GGCC 3 cut(s) 171, 176, 249
Cac8I GCNNGC 2 cut(s) 7, 91
CfoI GCGC 1 cut(s) 575
Cfr10I RCCGGY 2 cut(s) 43, 502
Cfr13I GGNCC 3 cut(s) 46, 169, 248
Csp6I GTAC 3 cut(s) 68, 376, 469
CspAI ACCGGT 1 cut(s) 502
CviAII CATG 3 cut(s) 331, 466, 646
CviQI GTAC 3 cut(s) 68, 376, 469
DdeI CTNAG 2 cut(s) 222, 597
DinI GGCGCC 1 cut(s) 574
DpnI GATC 2 cut(s) 387, 651
DpnII GATC 2 cut(s) 385, 649
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
EciI GGCGGA 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 171, 390
EcoT14I CCWWGG 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 574
EheI GGCGCC 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 3 cut(s) 334, 469, 649
FaiI YATR 5 cut(s) 332, 432, 467, 610, 647
FaqI GGGAC 1 cut(s) 336
FatI CATG 3 cut(s) 330, 465, 645
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 41, 449, 498
Fsp4HI GCNGC 3 cut(s) 41, 449, 498
GlaI GCGC 1 cut(s) 574
GluI GCNGC 3 cut(s) 41, 449, 498
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 3 cut(s) 171, 176, 249
HapII CCGG 2 cut(s) 44, 503
HhaI GCGC 1 cut(s) 575
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 3 cut(s) 334, 469, 649
Hin6I GCGC 1 cut(s) 573
HinP1I GCGC 1 cut(s) 573
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 2 cut(s) 27, 324
HpaII CCGG 2 cut(s) 44, 503
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 6 cut(s) 34, 63, 315, 344, 437, 600
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 3 cut(s) 248, 512, 560
HpyAV CCTTC 3 cut(s) 59, 273, 432
HpyCH4III ACNGT 2 cut(s) 556, 628
HpyCH4V TGCA 5 cut(s) 164, 185, 204, 284, 497
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 99, 108, 117, 182, 201
HpyF3I CTNAG 2 cut(s) 222, 597
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 3 cut(s) 334, 469, 649
HspAI GCGC 1 cut(s) 573
KasI GGCGCC 1 cut(s) 572
Kzo9I GATC 2 cut(s) 385, 649
Lsp1109I GCAGC 2 cut(s) 27, 509
MalI GATC 2 cut(s) 387, 651
MboI GATC 2 cut(s) 385, 649
MboII GAAGA 3 cut(s) 168, 304, 328
MluCI AATT 2 cut(s) 159, 279
Mly113I GGCGCC 1 cut(s) 573
MlyI GAGTC 2 cut(s) 36, 318
MnlI CCTC 8 cut(s) 187, 217, 261, 312, 348, 602, 606, 634
MseI TTAA 2 cut(s) 20, 369
MslI CAYNNNNRTG 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 2 cut(s) 44, 503
MspR9I CCNGG 2 cut(s) 173, 392
MvaI CCWGG 2 cut(s) 173, 392
MvnI CGCG 2 cut(s) 304, 588
MwoI GCNNNNNNNGC 6 cut(s) 11, 99, 108, 117, 182, 201
NarI GGCGCC 1 cut(s) 573
NdeII GATC 2 cut(s) 385, 649
NlaIII CATG 3 cut(s) 334, 469, 649
NlaIV GGNNCC 2 cut(s) 346, 574
PflFI GACNNNGTC 1 cut(s) 554
PinAI ACCGGT 1 cut(s) 502
PkrI GCNGC 3 cut(s) 42, 450, 499
PleI GAGTC 2 cut(s) 35, 318
PluTI GGCGCC 1 cut(s) 576
PpsI GAGTC 2 cut(s) 35, 318
Psp6I CCWGG 2 cut(s) 171, 390
PspGI CCWGG 2 cut(s) 171, 390
PspN4I GGNNCC 2 cut(s) 346, 574
PspPI GGNCC 3 cut(s) 46, 169, 248
PsrI GAACNNNNNNTAC 2 cut(s) 126, 158
PstI CTGCAG 1 cut(s) 499
PsyI GACNNNGTC 1 cut(s) 554
RsaI GTAC 3 cut(s) 69, 377, 470
RsaNI GTAC 3 cut(s) 68, 376, 469
RseI CAYNNNNRTG 1 cut(s) 464
SalI GTCGAC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 20, 369
SatI GCNGC 3 cut(s) 41, 449, 498
Sau3AI GATC 2 cut(s) 385, 649
Sau96I GGNCC 3 cut(s) 46, 169, 248
SchI GAGTC 2 cut(s) 36, 318
ScrFI CCNGG 2 cut(s) 173, 392
SetI ASST 6 cut(s) 59, 95, 443, 567, 594, 645
SfcI CTRYAG 1 cut(s) 495
SfoI GGCGCC 1 cut(s) 574
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 159, 279
SsiI CCGC 3 cut(s) 304, 401, 448
SspDI GGCGCC 1 cut(s) 572
StyD4I CCNGG 2 cut(s) 171, 390
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 2 cut(s) 556, 628
TaqI TCGA 5 cut(s) 243, 480, 507, 558, 652
TasI AATT 2 cut(s) 159, 279
TatI WGTACW 1 cut(s) 468
TauI GCSGC 1 cut(s) 451
Tru1I TTAA 2 cut(s) 20, 369
Tru9I TTAA 2 cut(s) 20, 369
TseI GCWGC 2 cut(s) 40, 497
TspDTI ATGAA 1 cut(s) 454
Tth111I GACNNNGTC 1 cut(s) 554
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.