MD03G1217100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016789
Physical Location & Seq
Reverse (-)
30122258 .. 30125211
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1217100.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGCATCGCGAAAACAACAGCCCACTGTGTCACGCTAGCACAACAAGTGCCAATGGATATTCTACAACGACCCAAGTGAGGACGAAGAAGAAGAAAAAGAAAGCTACAAATTTCTTCCTATGTTTTCGATCGTTTGATGTGGTCGGTTCAGAACCTGGACGACCTTCTAGGAATGTTTTCACATATGTGGCTGTGGGCGAGAGGGAAGCTGGGGTGTTTCCCAAGGTGGTGCCAGTGTCGTCGTCGCTGGCAACCTCAGATAATGAAGGAGACAATGAAAAATGTCGTAGTACTCAAAAAGAAAAGAAAACACGATGTGGATTTTATCAAGTTTTAAAGGCAGTTTTGTTCAAAACAAAATTGGTCAATAAGATTAAAGAAAGAAACTCGGAAAAGAATTTTGCAAACACAACACATGAGAGTTCAATTACCTCAACTCCTAATCGACGTCGGTCGGAGGATGTGCCGAAAGTGATCTCCAAGAAAAATTCATACGTTGGCGTGGAAGAAAAGCAAGGTGTGGTGCTCCCGAAGATGTCACCAATGGCTTCACCATTGCTAGGTAATGAAGAAAATGATGAGAAACGTGACAGTCAATATTGGAGAAACAAGGGCCGACGTAAATTATCTCGTGTTTTGAAGGCGATTATGTTCGAGACATCTTTGGCCAAGAAGATTCAAAAAAGAAAGAAGGGAAATAAACTTGGAAGAAATGAGAGTTCAAAGTACAACTCCGAGGAAGATGACCCAAGAGTGATGTCTGACACTGCTTCTTCTTTAACTAACTGTTCTGTCTTCATTTCATCTTCTGTAAACTCTTCATCAAGTTCGTTATCGCAAACGTCCAATTCAAGCATCAGGTCTTTGTCCAAGAGGATCAAATCTCTTCGATCAAGCTCATCGTGTAATTTTAAAGCCAATTTGAAGTACTTGTCCGGCAAATTTCGACCAACTTCGACTAGTAACAATTCAAGATCTAATTATGCTAAAAACAAGTCGTTTGAAGCAAATACAAATGCTTCAAAAGAAGGGCAAGGACAAAGAGACCTAGATGTAACCAGGATTGAGAAGAACAAGTACAAAAAAGGATGTTACAGATCCATCGCGAGTTTGGGTTTCCTGCTCATGGGTTTGTTGGTTTTGATATTTTACGGTAAGATTTATGCCATTTTATGCACTTTGGTGTCGCTCTTTAGTGTGCCTCTTTGGTGTTCCAGTGTTGGACAGCACCATGGTTCAACAAACAAGATTGACAAGAAGAAATTCATCATTGGAGGATTACTACCAAGAGACTCGAACAATTCACTGCAAATGACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

49.14

Weight (kDa)

10.07

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016789)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21200
malus_domestica MD03G1217100.v1.1 MD11G1234800.v1.1
prunus_persica Prupe.4G192300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0036261
rosa_laevigata RLG00000033682
rosa_multiflora Rmu_sc0000247.1_g000012
rosa_roxburghii Rroxscaffold_1G00044530
rosa_rugosa Rorug05G0154400
rosa_samantha Rh5AG248100 Rh5BG248700 Rh5CG280000 Rh5DG257000
rosa_wichuraiana Rw5G022750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 229
AccII CGCG 2 cut(s) 9, 1106
AclWI GGATC 2 cut(s) 884, 1092
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 5 cut(s) 109, 397, 487, 941, 1262
AcyI GRCGYC 1 cut(s) 448
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 4 cut(s) 292, 728, 929, 1079
AfiI CCNNNNNNNGG 3 cut(s) 78, 560, 1126
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 2 cut(s) 154, 1058
AleI CACNNNNGTG 2 cut(s) 185, 1181
AluBI AGCT 3 cut(s) 104, 209, 897
AluI AGCT 3 cut(s) 104, 209, 897
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 4 cut(s) 264, 650, 1038, 1284
AlwI GGATC 2 cut(s) 884, 1092
AlwNI CAGNNNCTG 1 cut(s) 155
AoxI GGCC 2 cut(s) 613, 666
ApoI RAATTY 5 cut(s) 109, 397, 487, 941, 1262
Asp700I GAANNNNTTC 2 cut(s) 176, 1262
AspS9I GGNCC 1 cut(s) 613
AsuHPI GGTGA 2 cut(s) 531, 543
AsuNHI GCTAGC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 668
BanI GGYRCC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 1061, 1093
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 787
Bbv12I GWGCWC 1 cut(s) 528
BccI CCATC 1 cut(s) 1109
BciT130I CCWGG 2 cut(s) 156, 1060
BcoDI GTCTC 4 cut(s) 264, 650, 1038, 1284
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 5 cut(s) 36, 168, 560, 960, 1049
BglII AGATCT 1 cut(s) 974
BmcAI AGTACT 2 cut(s) 292, 929
Bme1390I CCNGG 2 cut(s) 156, 1060
BmgT120I GGNCC 1 cut(s) 613
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 2 cut(s) 156, 1060
BmsI GCATC 2 cut(s) 13, 864
BmtI GCTAGC 1 cut(s) 39
BoxI GACNNNNGTC 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 787
BsaHI GRCGYC 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 1038
BsaJI CCNNGG 3 cut(s) 222, 735, 1231
BsaXI ACNNNNNCTCC 2 cut(s) 421, 451
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 560, 1126
Bse1I ACTGG 2 cut(s) 233, 1215
Bse3DI GCAATG 1 cut(s) 554
BseBI CCWGG 2 cut(s) 156, 1060
BseDI CCNNGG 3 cut(s) 222, 735, 1231
BseGI GGATG 2 cut(s) 466, 1094
BseLI CCNNNNNNNGG 3 cut(s) 78, 560, 1126
BseMI GCAATG 1 cut(s) 554
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 2 cut(s) 233, 1215
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 9, 1106
Bsh1285I CGRYCG 2 cut(s) 131, 455
BshFI GGCC 2 cut(s) 615, 668
BshNI GGYRCC 1 cut(s) 229
BsiEI CGRYCG 2 cut(s) 131, 455
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 1 cut(s) 936
BslI CCNNNNNNNGG 3 cut(s) 78, 560, 1126
BsmAI GTCTC 4 cut(s) 264, 650, 1038, 1284
BsnI GGCC 2 cut(s) 615, 668
Bso31I GGTCTC 1 cut(s) 1038
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
Bsp19I CCATGG 1 cut(s) 1231
Bsp68I TCGCGA 2 cut(s) 9, 1106
BspANI GGCC 2 cut(s) 615, 668
BspCNI CTCAG 1 cut(s) 269
BspFNI CGCG 2 cut(s) 9, 1106
BspLI GGNNCC 1 cut(s) 231
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 2 cut(s) 884, 1092
BspT107I GGYRCC 1 cut(s) 229
BspTNI GGTCTC 1 cut(s) 1038
BsrDI GCAATG 1 cut(s) 554
BsrI ACTGG 2 cut(s) 233, 1215
BssECI CCNNGG 3 cut(s) 222, 735, 1231
BssMI GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
BssNI GRCGYC 1 cut(s) 448
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 2 cut(s) 222, 1231
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 2 cut(s) 156, 1060
Bst4CI ACNGT 4 cut(s) 27, 593, 788, 1154
Bst6I CTCTTC 2 cut(s) 823, 891
BstACI GRCGYC 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 37, 249
BstDEI CTNAG 1 cut(s) 256
BstDSI CCRYGG 1 cut(s) 1231
BstF5I GGATG 2 cut(s) 466, 1094
BstFNI CGCG 2 cut(s) 9, 1106
BstKTI GATC 6 cut(s) 131, 477, 879, 893, 977, 1100
BstMAI GTCTC 4 cut(s) 264, 650, 1038, 1284
BstMBI GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
BstMCI CGRYCG 2 cut(s) 131, 455
BstNI CCWGG 2 cut(s) 156, 1060
BstPAI GACNNNNGTC 1 cut(s) 451
BstSCI CCNGG 2 cut(s) 154, 1058
BstUI CGCG 2 cut(s) 9, 1106
BstV2I GAAGAC 1 cut(s) 787
BstX2I RGATCY 2 cut(s) 974, 1097
BstYI RGATCY 2 cut(s) 974, 1097
BsuRI GGCC 2 cut(s) 615, 668
BtgI CCRYGG 1 cut(s) 1231
BtgZI GCGATG 1 cut(s) 1087
BtsCI GGATG 2 cut(s) 466, 1094
BtsI GCAGTG 2 cut(s) 765, 1304
BtsIMutI CAGTG 5 cut(s) 23, 240, 765, 1222, 1304
BtuMI TCGCGA 2 cut(s) 9, 1106
Cac8I GCNNGC 2 cut(s) 37, 249
CaiI CAGNNNCTG 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 613
Csp6I GTAC 4 cut(s) 291, 727, 928, 1078
CviAII CATG 3 cut(s) 416, 1126, 1232
CviJI RGCY 9 cut(s) 21, 104, 191, 209, 548, 615, 668, 897, 917
CviKI_1 RGCY 9 cut(s) 21, 104, 191, 209, 548, 615, 668, 897, 917
CviQI GTAC 4 cut(s) 291, 727, 928, 1078
DdeI CTNAG 1 cut(s) 256
DpnI GATC 6 cut(s) 130, 476, 878, 892, 976, 1099
DpnII GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
DraI TTTAAA 2 cut(s) 336, 913
DraIII CACNNNGTG 1 cut(s) 317
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 2 cut(s) 823, 891
EarI CTCTTC 2 cut(s) 823, 891
Eco130I CCWWGG 2 cut(s) 222, 1231
Eco31I GGTCTC 1 cut(s) 1038
EcoRII CCWGG 2 cut(s) 154, 1058
EcoT14I CCWWGG 2 cut(s) 222, 1231
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 222, 1231
FaeI CATG 3 cut(s) 419, 1129, 1235
FatI CATG 3 cut(s) 415, 1125, 1231
FauNDI CATATG 1 cut(s) 184
FokI GGATG 2 cut(s) 473, 1101
FspBI CTAG 5 cut(s) 36, 168, 560, 960, 1049
GsaI CCCAGC 1 cut(s) 213
HaeIII GGCC 2 cut(s) 615, 668
HapII CCGG 1 cut(s) 936
Hin1I GRCGYC 1 cut(s) 448
Hin1II CATG 3 cut(s) 419, 1129, 1235
HinfI GANTC 2 cut(s) 676, 1292
HpaII CCGG 1 cut(s) 936
HphI GGTGA 2 cut(s) 531, 543
Hpy166II GTNNAC 1 cut(s) 814
Hpy188I TCNGA 6 cut(s) 151, 259, 391, 457, 736, 763
Hpy188III TCNNGA 5 cut(s) 8, 529, 655, 972, 1105
Hpy8I GTNNAC 1 cut(s) 814
Hpy99I CGWCG 5 cut(s) 244, 247, 450, 453, 621
HpyAV CCTTC 5 cut(s) 174, 260, 634, 685, 1022
HpyCH4III ACNGT 4 cut(s) 27, 593, 788, 1154
HpyCH4IV ACGT 5 cut(s) 448, 495, 586, 619, 842
HpyCH4V TGCA 4 cut(s) 4, 404, 1176, 1309
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 5 cut(s) 448, 495, 586, 619, 842
Hsp92I GRCGYC 1 cut(s) 448
Hsp92II CATG 3 cut(s) 419, 1129, 1235
Kzo9I GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
LmnI GCTCC 1 cut(s) 531
LweI GCATC 2 cut(s) 13, 864
MaeI CTAG 5 cut(s) 36, 168, 560, 960, 1049
MaeII ACGT 5 cut(s) 448, 495, 586, 619, 842
MaeIII GTNAC 6 cut(s) 29, 537, 587, 962, 1054, 1091
MalI GATC 6 cut(s) 130, 476, 878, 892, 976, 1099
MboI GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
MflI RGATCY 2 cut(s) 974, 1097
MhlI GDGCHC 1 cut(s) 528
MlsI TGGCCA 1 cut(s) 668
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 1 cut(s) 1286
MmeI TCCRAC 2 cut(s) 435, 1201
MnlI CCTC 9 cut(s) 72, 195, 265, 442, 451, 730, 867, 1212, 1268
Mox20I TGGCCA 1 cut(s) 668
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 176, 1262
MscI TGGCCA 1 cut(s) 668
MseI TTAA 5 cut(s) 335, 375, 779, 912, 1321
MslI CAYNNNNRTG 2 cut(s) 185, 1181
Msp20I TGGCCA 1 cut(s) 668
MspI CCGG 1 cut(s) 936
MspR9I CCNGG 2 cut(s) 156, 1060
MvaI CCWGG 2 cut(s) 156, 1060
MvnI CGCG 2 cut(s) 9, 1106
NcoI CCATGG 1 cut(s) 1231
NdeI CATATG 1 cut(s) 184
NdeII GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
NheI GCTAGC 1 cut(s) 35
NlaIII CATG 3 cut(s) 419, 1129, 1235
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 3 cut(s) 29, 537, 587
NruI TCGCGA 2 cut(s) 9, 1106
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 2 cut(s) 185, 1181
PdmI GAANNNNTTC 2 cut(s) 176, 1262
PfeI GAWTC 1 cut(s) 676
Ple19I CGATCG 1 cut(s) 131
PleI GAGTC 1 cut(s) 1286
PpsI GAGTC 1 cut(s) 1286
PshAI GACNNNNGTC 1 cut(s) 451
Psp6I CCWGG 2 cut(s) 154, 1058
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 2 cut(s) 154, 1058
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 613
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 2 cut(s) 974, 1097
PvuI CGATCG 1 cut(s) 131
RruI TCGCGA 2 cut(s) 9, 1106
RsaI GTAC 4 cut(s) 292, 728, 929, 1079
RsaNI GTAC 4 cut(s) 291, 727, 928, 1078
RseI CAYNNNNRTG 2 cut(s) 185, 1181
SaqAI TTAA 5 cut(s) 335, 375, 779, 912, 1321
Sau3AI GATC 6 cut(s) 128, 474, 876, 890, 974, 1097
Sau96I GGNCC 1 cut(s) 613
ScaI AGTACT 2 cut(s) 292, 929
SchI GAGTC 1 cut(s) 1286
ScrFI CCNGG 2 cut(s) 156, 1060
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 2 cut(s) 13, 864
SmiMI CAYNNNNRTG 2 cut(s) 185, 1181
SpeI ACTAGT 1 cut(s) 959
SspI AATATT 1 cut(s) 599
SspMI CTAG 5 cut(s) 36, 168, 560, 960, 1049
StyD4I CCNGG 2 cut(s) 154, 1058
StyI CCWWGG 2 cut(s) 222, 1231
TaaI ACNGT 4 cut(s) 27, 593, 788, 1154
TaiI ACGT 5 cut(s) 451, 498, 589, 622, 845
TaqI TCGA 7 cut(s) 127, 445, 654, 889, 946, 956, 1295
TaqII GACCGA 1 cut(s) 441
TatI WGTACW 4 cut(s) 290, 726, 927, 1077
TfiI GAWTC 1 cut(s) 676
Tru1I TTAA 5 cut(s) 335, 375, 779, 912, 1321
Tru9I TTAA 5 cut(s) 335, 375, 779, 912, 1321
TscAI CASTG 5 cut(s) 30, 240, 772, 1222, 1311
TseFI GTSAC 3 cut(s) 29, 537, 587
Tsp45I GTSAC 3 cut(s) 29, 537, 587
TspDTI ATGAA 8 cut(s) 279, 291, 480, 582, 787, 792, 810, 1255
TspRI CASTG 5 cut(s) 30, 240, 772, 1222, 1311
XapI RAATTY 5 cut(s) 109, 397, 487, 941, 1262
XcmI CCANNNNNNNNNTGG 1 cut(s) 1108
XmnI GAANNNNTTC 2 cut(s) 176, 1262
XspI CTAG 5 cut(s) 36, 168, 560, 960, 1049
ZraI GACGTC 1 cut(s) 449
ZrmI AGTACT 2 cut(s) 292, 929
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.