MD11G1234800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016789
Physical Location & Seq
Reverse (-)
34189893 .. 34192162
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1234800.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGCATCGCGAAAACCACAGCCCGCTATGTCCCCCAAGTACAACAAGTGCCAATAGATATTCGACAACGACCCAAGTGAAGACCAAGAAGAAGAAAAATAAAATAACCAATTTCTTCCCATGCTTTCGATCGTCTGATGTGACTGTTTTGGAACTAGGACGACTTCCTAAGAATGTTTTCACATACATGGCTATAGATGATAAGGAACGTGGGGTGTTTCCTAAGGTGGTACCAATGTCTTTATCGTCGTCGTTGGCAACATCAAATGATGAAGGAGATGATGAAAAATGTCACTGTACTCAACAAGAAGAGAAAGGACGATGTGGATTTTCTCAAGTTTTAAAGGCAGTTTTGGTCAAAACAAAACTGGTTAAGAAGATCCATGAAAGAAAATTTGAAACGAATTTTGCTAACACAACAAATGAGAGTTCAATTACCTCAACTTCTAATCTAGATTGGTCGGAGGGAGATGTGCCGAAAGTGATCTCCAAGAAAAGGTCATACATTGGCATGGAAGAAAAGGGAGGTTTGGTTTTCCCGAAAGTGTCACCAATGGCTTCACCATTGCTATGTAATGAAGAAAATAATGACAAACGTGACAGTCAATATTGGAGAAGTAAAGGCCGAAATAAATTATCGAATGTTTTGAAGGCGATTTTGTTCGATACATCTTTGGCCAAGAAGATTCAAAAAAGAAAGAAGGGAAAGAAACTTGGAAGAAATGAGAGTTCAAAGTACAATTCCAAGGAAGATGACCCAAAAGTGATGTCTGACACTGCTTCTTTTTTAACTAATTGTTCTATCTTCACTTCATCTTCTATAAACTCTTCATCAAGTTCGATATCGCAAACATCCAATTCAAGCATCAGGTATTTGTCCAAGAGGATCAAATCTTTTCGATCAAGCTCGTCTTGTAATTTTAAAGCCAATTTGAGGTACTTGTCCGTCAAATTTCGATCAACTTCAACTAGTACTAGTTCGAAGGCCAATTCAAGGTCTAATTCTGATAAACACAAATTGTTTGAAGCAAATAAAAATGCTTCAAAAGAAGGGCAAGGACAACAAGACCAAGATGTTACCAGGATTGAGAAGAACAAGTACAAAAAAGTATGTTACAGATCGATTGTGAGTTTGTGTTTCCTGCTCATGGTTTTGTGGGGTTTGATGTTTTATGGTAAGATTTGTGCCATTTTATGTACTTTGGTGTGGCTCTTCAGTGTGCCTCTTTGGTGTACAAGTGTTGGACATCATGGTTTAACAAACAAGATTGACAAGAAGAAATTCATCATTGGAGGGTTGCTACCAAGAGACACTCAAACAATTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

443

Amino Acids

49.8

Weight (kDa)

9.8

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016789)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21200
malus_domestica MD03G1217100.v1.1 MD11G1234800.v1.1
prunus_persica Prupe.4G192300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0036261
rosa_laevigata RLG00000033682
rosa_multiflora Rmu_sc0000247.1_g000012
rosa_roxburghii Rroxscaffold_1G00044530
rosa_rugosa Rorug05G0154400
rosa_samantha Rh5AG248100 Rh5BG248700 Rh5CG280000 Rh5DG257000
rosa_wichuraiana Rw5G022750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 229
AccB1I GGYRCC 1 cut(s) 229
AccII CGCG 1 cut(s) 9
AciI CCGC 1 cut(s) 23
AclWI GGATC 2 cut(s) 373, 893
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 4 cut(s) 392, 403, 950, 1280
AcuI CTGAAG 1 cut(s) 1198
AfaI GTAC 9 cut(s) 40, 231, 298, 737, 938, 973, 1100, 1198, 1234
AfiI CCNNNNNNNGG 4 cut(s) 495, 933, 993, 1147
AhlI ACTAGT 2 cut(s) 968, 974
AjnI CCWGG 1 cut(s) 1079
AluBI AGCT 1 cut(s) 906
AluI AGCT 1 cut(s) 906
Alw26I GTCTC 1 cut(s) 1302
AlwI GGATC 2 cut(s) 373, 893
AoxI GGCC 3 cut(s) 622, 675, 984
ApoI RAATTY 4 cut(s) 392, 403, 950, 1280
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
Asp700I GAANNNNTTC 2 cut(s) 176, 1280
Asp718I GGTACC 1 cut(s) 229
AsuHPI GGTGA 2 cut(s) 540, 552
AsuII TTCGAA 1 cut(s) 980
AxyI CCTNAGG 1 cut(s) 222
BaeI ACNNNNGTAYC 2 cut(s) 221, 254
BalI TGGCCA 1 cut(s) 677
BanI GGYRCC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 1082, 1114
BbsI GAAGAC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 1081
BcoDI GTCTC 1 cut(s) 1302
BcuI ACTAGT 2 cut(s) 968, 974
BfaI CTAG 4 cut(s) 155, 452, 969, 975
BfmI CTRYAG 1 cut(s) 192
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 1 cut(s) 1081
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 1081
BmsI GCATC 2 cut(s) 13, 873
BpiI GAAGAC 1 cut(s) 86
Bpu14I TTCGAA 1 cut(s) 980
BpuEI CTTGAG 1 cut(s) 318
Bsa29I ATCGAT 1 cut(s) 1121
BsaJI CCNNGG 1 cut(s) 744
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 4 cut(s) 495, 933, 993, 1147
Bse1I ACTGG 1 cut(s) 372
Bse21I CCTNAGG 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 563
BseBI CCWGG 1 cut(s) 1081
BseCI ATCGAT 1 cut(s) 1121
BseDI CCNNGG 1 cut(s) 744
BseGI GGATG 1 cut(s) 851
BseLI CCNNNNNNNGG 4 cut(s) 495, 933, 993, 1147
BseMI GCAATG 1 cut(s) 563
BseNI ACTGG 1 cut(s) 372
Bsh1236I CGCG 1 cut(s) 9
Bsh1285I CGRYCG 1 cut(s) 131
BshFI GGCC 3 cut(s) 624, 677, 986
BshNI GGYRCC 1 cut(s) 229
BshVI ATCGAT 1 cut(s) 1121
BsiEI CGRYCG 1 cut(s) 131
BslFI GGGAC 1 cut(s) 15
BslI CCNNNNNNNGG 4 cut(s) 495, 933, 993, 1147
BsmAI GTCTC 1 cut(s) 1302
BsmFI GGGAC 1 cut(s) 15
BsnI GGCC 3 cut(s) 624, 677, 986
Bsp119I TTCGAA 1 cut(s) 980
Bsp1407I TGTACA 1 cut(s) 1232
Bsp143I GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
Bsp68I TCGCGA 1 cut(s) 9
BspACI CCGC 1 cut(s) 23
BspANI GGCC 3 cut(s) 624, 677, 986
BspDI ATCGAT 1 cut(s) 1121
BspFNI CGCG 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 2 cut(s) 373, 893
BspQI GCTCTTC 1 cut(s) 1217
BspT104I TTCGAA 1 cut(s) 980
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 563
BsrGI TGTACA 1 cut(s) 1232
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 1 cut(s) 744
BssMI GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
BssT1I CCWWGG 1 cut(s) 744
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 3 cut(s) 145, 296, 602
Bst6I CTCTTC 3 cut(s) 303, 832, 1217
BstAUI TGTACA 1 cut(s) 1232
BstBI TTCGAA 1 cut(s) 980
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 168, 222
BstF5I GGATG 1 cut(s) 851
BstFNI CGCG 1 cut(s) 9
BstKTI GATC 7 cut(s) 131, 381, 486, 888, 902, 959, 1121
BstMAI GTCTC 1 cut(s) 1302
BstMBI GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
BstMCI CGRYCG 1 cut(s) 131
BstNI CCWGG 1 cut(s) 1081
BstSCI CCNGG 1 cut(s) 1079
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 1 cut(s) 9
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 378
BstYI RGATCY 1 cut(s) 378
Bsu15I ATCGAT 1 cut(s) 1121
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 3 cut(s) 624, 677, 986
BsuTUI ATCGAT 1 cut(s) 1121
BtsCI GGATG 1 cut(s) 851
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 4 cut(s) 292, 774, 1222, 1324
BtuMI TCGCGA 1 cut(s) 9
Cac8I GCNNGC 1 cut(s) 23
ClaI ATCGAT 1 cut(s) 1121
Csp6I GTAC 9 cut(s) 39, 230, 297, 736, 937, 972, 1099, 1197, 1233
CviAII CATG 6 cut(s) 120, 187, 383, 511, 1147, 1250
CviJI RGCY 9 cut(s) 21, 191, 557, 624, 677, 906, 926, 986, 1210
CviKI_1 RGCY 9 cut(s) 21, 191, 557, 624, 677, 906, 926, 986, 1210
CviQI GTAC 9 cut(s) 39, 230, 297, 736, 937, 972, 1099, 1197, 1233
DdeI CTNAG 2 cut(s) 168, 222
DpnI GATC 7 cut(s) 130, 380, 485, 887, 901, 958, 1120
DpnII GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
DraI TTTAAA 2 cut(s) 342, 922
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 3 cut(s) 303, 832, 1217
EarI CTCTTC 3 cut(s) 303, 832, 1217
Eco130I CCWWGG 1 cut(s) 744
Eco32I GATATC 1 cut(s) 843
Eco57I CTGAAG 1 cut(s) 1198
Eco81I CCTNAGG 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 1079
EcoRV GATATC 1 cut(s) 843
EcoT14I CCWWGG 1 cut(s) 744
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 744
FaeI CATG 6 cut(s) 123, 190, 386, 514, 1150, 1253
FalI AAGNNNNNCTT 2 cut(s) 895, 927
FaqI GGGAC 1 cut(s) 15
FatI CATG 6 cut(s) 119, 186, 382, 510, 1146, 1249
FauI CCCGC 1 cut(s) 30
FokI GGATG 1 cut(s) 838
FspBI CTAG 4 cut(s) 155, 452, 969, 975
HaeIII GGCC 3 cut(s) 624, 677, 986
Hin1II CATG 6 cut(s) 123, 190, 386, 514, 1150, 1253
HinfI GANTC 1 cut(s) 685
HphI GGTGA 2 cut(s) 540, 552
Hpy166II GTNNAC 1 cut(s) 1233
Hpy188I TCNGA 4 cut(s) 136, 463, 772, 1006
Hpy188III TCNNGA 3 cut(s) 8, 452, 538
Hpy8I GTNNAC 1 cut(s) 1233
Hpy99I CGWCG 2 cut(s) 250, 253
HpyAV CCTTC 5 cut(s) 266, 643, 694, 976, 1043
HpyCH4III ACNGT 3 cut(s) 145, 296, 602
HpyCH4IV ACGT 2 cut(s) 208, 595
HpyCH4V TGCA 1 cut(s) 4
HpyF3I CTNAG 2 cut(s) 168, 222
HpySE526I ACGT 2 cut(s) 208, 595
Hsp92II CATG 6 cut(s) 123, 190, 386, 514, 1150, 1253
KpnI GGTACC 1 cut(s) 233
Kzo9I GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
LguI GCTCTTC 1 cut(s) 1217
LpnPI CCDG 5 cut(s) 353, 853, 1066, 1093, 1154
LweI GCATC 2 cut(s) 13, 873
MaeI CTAG 4 cut(s) 155, 452, 969, 975
MaeII ACGT 2 cut(s) 208, 595
MaeIII GTNAC 6 cut(s) 139, 290, 546, 596, 1075, 1112
MalI GATC 7 cut(s) 130, 380, 485, 887, 901, 958, 1120
MboI GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
MflI RGATCY 1 cut(s) 378
MlsI TGGCCA 1 cut(s) 677
MluNI TGGCCA 1 cut(s) 677
MmeI TCCRAC 2 cut(s) 441, 1222
MnlI CCTC 7 cut(s) 448, 457, 518, 876, 927, 1233, 1286
Mox20I TGGCCA 1 cut(s) 677
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 176, 1280
MscI TGGCCA 1 cut(s) 677
MseI TTAA 5 cut(s) 341, 372, 788, 921, 1256
MslI CAYNNNNRTG 3 cut(s) 185, 509, 568
Msp20I TGGCCA 1 cut(s) 677
MspR9I CCNGG 1 cut(s) 1081
MvaI CCWGG 1 cut(s) 1081
MvnI CGCG 1 cut(s) 9
NdeII GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
NlaIII CATG 6 cut(s) 123, 190, 386, 514, 1150, 1253
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 4 cut(s) 139, 290, 546, 596
NruI TCGCGA 1 cut(s) 9
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 980
PciSI GCTCTTC 1 cut(s) 1217
PdmI GAANNNNTTC 2 cut(s) 176, 1280
PfeI GAWTC 1 cut(s) 685
Ple19I CGATCG 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 1079
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 1 cut(s) 231
PsuI RGATCY 1 cut(s) 378
PvuI CGATCG 1 cut(s) 131
RruI TCGCGA 1 cut(s) 9
RsaI GTAC 9 cut(s) 40, 231, 298, 737, 938, 973, 1100, 1198, 1234
RsaNI GTAC 9 cut(s) 39, 230, 297, 736, 937, 972, 1099, 1197, 1233
RseI CAYNNNNRTG 3 cut(s) 185, 509, 568
SapI GCTCTTC 1 cut(s) 1217
SaqAI TTAA 5 cut(s) 341, 372, 788, 921, 1256
Sau3AI GATC 7 cut(s) 128, 378, 483, 885, 899, 956, 1118
ScaI AGTACT 1 cut(s) 973
ScrFI CCNGG 1 cut(s) 1081
SfaNI GCATC 2 cut(s) 13, 873
SfcI CTRYAG 1 cut(s) 192
SfuI TTCGAA 1 cut(s) 980
SmiMI CAYNNNNRTG 3 cut(s) 185, 509, 568
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
SpeI ACTAGT 2 cut(s) 968, 974
SsiI CCGC 1 cut(s) 23
SspI AATATT 1 cut(s) 608
SspMI CTAG 4 cut(s) 155, 452, 969, 975
StyD4I CCNGG 1 cut(s) 1079
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 3 cut(s) 145, 296, 602
TaiI ACGT 2 cut(s) 211, 598
TaqI TCGA 9 cut(s) 62, 127, 638, 663, 839, 898, 955, 980, 1121
TatI WGTACW 7 cut(s) 38, 296, 735, 971, 1098, 1196, 1232
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 5 cut(s) 341, 372, 788, 921, 1256
Tru9I TTAA 5 cut(s) 341, 372, 788, 921, 1256
TscAI CASTG 3 cut(s) 299, 781, 1222
TseFI GTSAC 4 cut(s) 139, 290, 546, 596
Tsp45I GTSAC 4 cut(s) 139, 290, 546, 596
TspDTI ATGAA 7 cut(s) 285, 297, 399, 591, 801, 819, 1273
TspGWI ACGGA 1 cut(s) 934
TspRI CASTG 3 cut(s) 299, 781, 1222
XapI RAATTY 4 cut(s) 392, 403, 950, 1280
XbaI TCTAGA 1 cut(s) 451
XmnI GAANNNNTTC 2 cut(s) 176, 1280
XspI CTAG 4 cut(s) 155, 452, 969, 975
ZrmI AGTACT 1 cut(s) 973
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.