Rh5CG280000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0016789
Physical Location & Seq
Forward (+)
30534432 .. 30536764
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG280000.1

Sequence Viewer

Length: 1281 bp
ATGGATTTCGAAAAGGACACCGCCACCCTTCCTCTTGCGTGCACTGCCCATGGATGTCCTGCAAAGGCCCAAGTGAGGCAGAAGAAGAAGAAGACGAGCGCTGTCAAGTTCTTCCTATGCTTTCAAAGAGAGGATGTAGTGTTTCCGTGTGTGGTGCCGCTGCCCGTGCCGGTGGTTTCGTCATCCATGGCGACATCGGATAGTGATTTGGATGTAGGAAAATGCAACAATCAACAAAGGAAGCCAGAACGGCATGGATTCTTTCATGTTTTGAAAGCAATTTTGTTCAAGATGTTATTGGTCAAGAAGATTCAACAAAAGAAATCAGACAGCAGTTTTGCAAATATTCCAACAAATGAGAGTTCAATTACTTCAAGGCACACTCAAGAATGGTCAGATCACGAAGATGTCCCAAAAATGATCACCAAAACAACTTCAGGGGGTAAGACCGTTTTCACTTACGTCAAAGAGAAGGAAGAGGTTGTCTTTCCGACGACGCCTTCATCGTGGCTTAAAAATGAAGAAACATGTCAAGATCAATGGATCAACAAAGGACGACGTAGATTATCTCACATTTTAAAGGCCGTCTTGTTCGAGACATCACTGGCTAGGAAGATTCAAGCAAGAAAGTTGGGGAAAAAATACGAATCAAATACGACTTTAAGGTCTAGCACTGGAGAATCGTGCGAGGAAGATAATCATGACCTGGGAAAGTCTGCGTCTTCGTTAACTAACTCTTCTGTCTTCACTTCATCTTTATTGAACTCTACATCAAGTTCATCAACAGTGTCACGCTCCACATCACACTCGAGGTCTTTATCCGAGCGAATCAAATCTTTCCGATCAATCTCATCAAGCAATCTAAAAGCCAATTTGAGGAACTTGTCCGACAAAATTCGATCAAACTCCAACCATTTGAAGTCCAATTCGAGAAACAAGATGTCGTTGGAATCCATTAATTCGAGTGGTTTGAAAGAAGAGCAACATGCACATTTTAATGATGATGTGACTGACTCTGGAAAGAACAATAAGAAAGGAGGGTGTTGTCGTTCAGCTTCGGGTCTTTGCATCTTGCTAATGAGTCTAGTGGGGTTGATTTTTTATGGTAAGCTTTGTGCCATATTGTGTACTTCAATGTGGCTTGTTTGTGTACCTCTTTGGAGTTTCGGATATCATAACTCATCAACAAACAAGGTGGTTGAGAATAAGAAAGTCACTATGGGTGGAGGAATGCTAGGGGGGACTGCTCGAACTCGAACTCGAATTTTTCAGAGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

47.29

Weight (kDa)

9.66

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016789)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21200
malus_domestica MD03G1217100.v1.1 MD11G1234800.v1.1
prunus_persica Prupe.4G192300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0036261
rosa_laevigata RLG00000033682
rosa_multiflora Rmu_sc0000247.1_g000012
rosa_roxburghii Rroxscaffold_1G00044530
rosa_rugosa Rorug05G0154400
rosa_samantha Rh5AG248100 Rh5BG248700 Rh5CG280000 Rh5DG257000
rosa_wichuraiana Rw5G022750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 664
AccB1I GGYRCC 1 cut(s) 154
AciI CCGC 2 cut(s) 21, 158
AclWI GGATC 1 cut(s) 551
AcsI RAATTY 2 cut(s) 894, 1263
AcuI CTGAAG 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 497
AfaI GTAC 2 cut(s) 1129, 1152
AfeI AGCGCT 1 cut(s) 100
AfiI CCNNNNNNNGG 2 cut(s) 75, 876
AflIII ACRYGT 1 cut(s) 527
AjnI CCWGG 1 cut(s) 705
AluBI AGCT 2 cut(s) 1055, 1111
AluI AGCT 2 cut(s) 1055, 1111
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 590
Alw44I GTGCAC 1 cut(s) 40
AlwI GGATC 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 808
Aor51HI AGCGCT 1 cut(s) 100
AoxI GGCC 2 cut(s) 66, 582
ApaLI GTGCAC 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 2 cut(s) 894, 1263
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
AseI ATTAAT 1 cut(s) 957
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 1 cut(s) 67
AsuHPI GGTGA 1 cut(s) 415
AsuII TTCGAA 1 cut(s) 9
AvaI CYCGRG 1 cut(s) 808
BaeGI GKGCMC 1 cut(s) 44
BanI GGYRCC 1 cut(s) 154
BbsI GAAGAC 3 cut(s) 98, 714, 736
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 147
BceAI ACGGC 2 cut(s) 266, 569
BciT130I CCWGG 1 cut(s) 707
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 590
BfaI CTAG 4 cut(s) 609, 669, 1085, 1235
BfoI RGCGCY 1 cut(s) 102
BglI GCCNNNNNGGC 1 cut(s) 250
BisI GCNGC 2 cut(s) 158, 161
BlsI GCNGC 2 cut(s) 159, 162
Bme1390I CCNGG 1 cut(s) 707
BmeT110I CYCGRG 1 cut(s) 808
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 1 cut(s) 707
BmsI GCATC 1 cut(s) 1077
BpiI GAAGAC 3 cut(s) 98, 714, 736
BpmI CTGGAG 1 cut(s) 696
Bpu14I TTCGAA 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 49, 186, 706
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 876
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 609, 679
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 3 cut(s) 49, 186, 706
BseGI GGATG 4 cut(s) 59, 139, 182, 217
BseLI CCNNNNNNNGG 2 cut(s) 75, 876
BseNI ACTGG 2 cut(s) 609, 679
BseSI GKGCMC 1 cut(s) 44
BseXI GCAGC 1 cut(s) 147
BshFI GGCC 2 cut(s) 68, 584
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 1 cut(s) 170
BslFI GGGAC 2 cut(s) 395, 1255
BslI CCNNNNNNNGG 2 cut(s) 75, 876
BsmAI GTCTC 1 cut(s) 590
BsmFI GGGAC 2 cut(s) 395, 1255
BsmI GAATGC 1 cut(s) 1236
BsnI GGCC 2 cut(s) 68, 584
BsoBI CYCGRG 1 cut(s) 808
Bsp119I TTCGAA 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 6 cut(s) 397, 420, 535, 543, 842, 899
Bsp19I CCATGG 2 cut(s) 49, 186
BspACI CCGC 2 cut(s) 21, 158
BspANI GGCC 2 cut(s) 68, 584
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 1 cut(s) 551
BspQI GCTCTTC 1 cut(s) 972
BspT104I TTCGAA 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 609, 679
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 49, 186, 706
BssMI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
BssNI GRCGYC 1 cut(s) 497
BssT1I CCWWGG 2 cut(s) 49, 186
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 2 cut(s) 451, 787
Bst6I CTCTTC 3 cut(s) 471, 742, 972
BstACI GRCGYC 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 40
BstDSI CCRYGG 2 cut(s) 49, 186
BstF5I GGATG 4 cut(s) 59, 139, 182, 217
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 6 cut(s) 400, 423, 538, 546, 845, 902
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
BstMWI GCNNNNNNNGC 3 cut(s) 44, 166, 250
BstNI CCWGG 1 cut(s) 707
BstNSI RCATGY 2 cut(s) 531, 989
BstSCI CCNGG 1 cut(s) 705
BstSLI GKGCMC 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 147
BstV2I GAAGAC 3 cut(s) 98, 714, 736
BsuRI GGCC 2 cut(s) 68, 584
BtgI CCRYGG 2 cut(s) 49, 186
BtsCI GGATG 4 cut(s) 59, 139, 182, 217
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 4 cut(s) 42, 602, 672, 792
Cac8I GCNNGC 1 cut(s) 40
CciI TCATGA 1 cut(s) 700
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 67
CseI GACGC 2 cut(s) 505, 708
Csp6I GTAC 2 cut(s) 1128, 1151
CspCI CAANNNNNGTGG 2 cut(s) 1176, 1211
CviAII CATG 7 cut(s) 50, 187, 254, 266, 528, 701, 986
CviJI RGCY 9 cut(s) 68, 244, 511, 584, 608, 869, 1055, 1111, 1141
CviKI_1 RGCY 9 cut(s) 68, 244, 511, 584, 608, 869, 1055, 1111, 1141
CviQI GTAC 2 cut(s) 1128, 1151
DpnI GATC 6 cut(s) 399, 422, 537, 545, 844, 901
DpnII GATC 6 cut(s) 397, 420, 535, 543, 842, 899
DraI TTTAAA 1 cut(s) 579
DrdI GACNNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 664
Eam1104I CTCTTC 3 cut(s) 471, 742, 972
EarI CTCTTC 3 cut(s) 471, 742, 972
Eco130I CCWWGG 2 cut(s) 49, 186
Eco32I GATATC 1 cut(s) 1172
Eco47III AGCGCT 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 808
EcoRII CCWGG 1 cut(s) 705
EcoRV GATATC 1 cut(s) 1172
EcoT14I CCWWGG 2 cut(s) 49, 186
ErhI CCWWGG 2 cut(s) 49, 186
FaeI CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FaqI GGGAC 2 cut(s) 395, 1255
FatI CATG 7 cut(s) 49, 186, 253, 265, 527, 700, 985
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 158, 161
FokI GGATG 4 cut(s) 66, 146, 169, 224
Fsp4HI GCNGC 2 cut(s) 158, 161
FspBI CTAG 4 cut(s) 609, 669, 1085, 1235
GlaI GCGC 1 cut(s) 100
GluI GCNGC 2 cut(s) 158, 161
GsuI CTGGAG 1 cut(s) 696
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 2 cut(s) 68, 584
HapII CCGG 1 cut(s) 170
HgaI GACGC 2 cut(s) 505, 708
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 497
Hin1II CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 1109
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 4 cut(s) 42, 729, 1128, 1151
Hpy188I TCNGA 9 cut(s) 199, 328, 397, 492, 823, 842, 889, 1169, 1272
Hpy188III TCNNGA 9 cut(s) 289, 304, 386, 401, 533, 595, 701, 930, 1017
Hpy8I GTNNAC 4 cut(s) 42, 729, 1128, 1151
Hpy99I CGWCG 3 cut(s) 496, 499, 561
HpyAV CCTTC 3 cut(s) 38, 466, 510
HpyCH4III ACNGT 2 cut(s) 451, 787
HpyCH4IV ACGT 2 cut(s) 462, 559
HpyCH4V TGCA 6 cut(s) 42, 62, 225, 341, 989, 1068
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 166, 250
HpySE526I ACGT 2 cut(s) 462, 559
Hsp92I GRCGYC 1 cut(s) 497
Hsp92II CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
HspAI GCGC 1 cut(s) 99
Ksp22I TGATCA 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 6 cut(s) 397, 420, 535, 543, 842, 899
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 1 cut(s) 800
LpnPI CCDG 9 cut(s) 72, 183, 258, 423, 590, 660, 692, 719, 1002
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 1 cut(s) 1077
MaeI CTAG 4 cut(s) 609, 669, 1085, 1235
MaeII ACGT 2 cut(s) 462, 559
MaeIII GTNAC 3 cut(s) 789, 1006, 1213
MalI GATC 6 cut(s) 399, 422, 537, 545, 844, 901
MboI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 7 cut(s) 279, 366, 871, 894, 925, 958, 1263
MlyI GAGTC 2 cut(s) 1007, 1090
MmeI TCCRAC 5 cut(s) 374, 515, 912, 927, 933
MseI TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
MslI CAYNNNNRTG 2 cut(s) 405, 996
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 1236
MvaI CCWGG 1 cut(s) 707
MwoI GCNNNNNNNGC 3 cut(s) 44, 166, 250
NcoI CCATGG 2 cut(s) 49, 186
NdeII GATC 6 cut(s) 397, 420, 535, 543, 842, 899
NlaIII CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 3 cut(s) 789, 1006, 1213
NspI RCATGY 2 cut(s) 531, 989
NspV TTCGAA 1 cut(s) 9
PaeR7I CTCGAG 1 cut(s) 808
PagI TCATGA 1 cut(s) 700
PciI ACATGT 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 972
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PctI GAATGC 1 cut(s) 1236
PfeI GAWTC 7 cut(s) 258, 310, 616, 647, 680, 828, 950
PkrI GCNGC 2 cut(s) 159, 162
PleI GAGTC 2 cut(s) 1007, 1089
PpsI GAGTC 2 cut(s) 1007, 1089
PscI ACATGT 1 cut(s) 527
PshBI ATTAAT 1 cut(s) 957
Psp6I CCWGG 1 cut(s) 705
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 1 cut(s) 67
PspXI VCTCGAGB 1 cut(s) 808
RsaI GTAC 2 cut(s) 1129, 1152
RsaNI GTAC 2 cut(s) 1128, 1151
RseI CAYNNNNRTG 2 cut(s) 405, 996
SapI GCTCTTC 1 cut(s) 972
SaqAI TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
SatI GCNGC 2 cut(s) 158, 161
Sau3AI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 2 cut(s) 1007, 1090
ScrFI CCNGG 1 cut(s) 707
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 1 cut(s) 1077
Sfr274I CTCGAG 1 cut(s) 808
SfuI TTCGAA 1 cut(s) 9
SlaI CTCGAG 1 cut(s) 808
SmiMI CAYNNNNRTG 2 cut(s) 405, 996
SmlI CTYRAG 2 cut(s) 384, 808
SmoI CTYRAG 2 cut(s) 384, 808
Sse9I AATT 7 cut(s) 279, 366, 871, 894, 925, 958, 1263
SsiI CCGC 2 cut(s) 21, 158
SspI AATATT 1 cut(s) 346
SspMI CTAG 4 cut(s) 609, 669, 1085, 1235
StyD4I CCNGG 1 cut(s) 705
StyI CCWWGG 2 cut(s) 49, 186
TaaI ACNGT 2 cut(s) 451, 787
TaiI ACGT 2 cut(s) 465, 562
TaqI TCGA 9 cut(s) 9, 594, 809, 898, 929, 962, 1249, 1255, 1261
TasI AATT 7 cut(s) 279, 366, 871, 894, 925, 958, 1263
TatI WGTACW 1 cut(s) 1127
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 7 cut(s) 258, 310, 616, 647, 680, 828, 950
Tru1I TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
Tru9I TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
TscAI CASTG 4 cut(s) 49, 609, 679, 792
TseFI GTSAC 3 cut(s) 789, 1006, 1213
TseI GCWGC 1 cut(s) 160
Tsp45I GTSAC 3 cut(s) 789, 1006, 1213
TspDTI ATGAA 5 cut(s) 254, 492, 534, 741, 768
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 4 cut(s) 49, 609, 679, 792
VneI GTGCAC 1 cut(s) 40
VspI ATTAAT 1 cut(s) 957
XapI RAATTY 2 cut(s) 894, 1263
XceI RCATGY 2 cut(s) 531, 989
XhoI CTCGAG 1 cut(s) 808
XspI CTAG 4 cut(s) 609, 669, 1085, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.