Rh5DG257000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016789
Physical Location & Seq
Forward (+)
30286349 .. 30288681
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG257000.1

Sequence Viewer

Length: 1281 bp
ATGGATTTCGAAAAGGACACCGCCACCCTTCCTCTTGCGTGCACTGCCCATGGATGTCCTGCAAAGGCCCAAGTGAGGCAAAAGAAGAAGAAGACGAGCGCTGTCAAGTTCTTCCTGTGCTTTCAAAGAGACGATGTAGTGTTTCCGTGTGTGGTGCCGATGCCCGTGCCGGCGGTTTCGTCATCCATGGCGACATCGGATAGTGATTTGGATGTAGGAAAATGCAACAATCAACAAAGGAAGCCAGAACGGCATGGATTCTTTCATGTTTTGAAAGCAATTTTGTTCAAGATGTTATTGGTCAAGAAGATTCAACAAAAGAAATCAGACAGCAGTTTTGCAAATATTCCAACAAAGGAGAGTTCAATTACTTCAAGGCACACTCAAGAATGGTCAGATCACAAAGATGTCCCAAAAATGATCACCAAAACAACTTCAGGGGGTAAGACCGTTTTCACTTACGTCAAAGAGAAGGAAGAGGTTGTCTTTCCGACGATGCCTTCATCGTGGCTTAAAAATGAAGAAACATGTCAAGATCAATGGATCAACAAAGGACGACGTAGATTATCTCACATTTTAAAGGCCGTCTTGTTCGAGACATCACTGGCTAGGAAGATTCAAGCAAGAAAGTTGGGGAAAAAATACGAATCAAATACGAGTTTAAGGTCTAGCACTGGAGAATCGTGCGAGGAAGATAATCATGACCTGGGAAAGTCTGCGTCTTCGTTAACTAACTCTTCTGTCTTCACTTCATCTTTATTGAACTCTACATCAAGTTCATCAACAGTGTCACGCTCCACATCACACTCGAGGTCTTTATCCGAGCGAATCAAATCTTTCCGATCAATCTCATCAAGCAATCTAAAAGCCAATTTGAGGAACTTGTCCGACAAAATTCGATCAAACTCCAACCATTTGAAGTCCAATTCGAGAAACAAGATGTCGTTGGAATCCATTAATTCGAGTGGTTTGAAAGAAGAGCAACATGCACATTTTAATGATGACGTGACTGACACTGGAAAGAACAATAAGAAAGGAGGGTGTTATCGTTCAGCTTCGGGCCTTTGCTTCTTGCTAATGAGTCTAGTGGGGTTGATTTTTTATGGTAAGCTTTGTGCCATATTGTGCACTTCAATGTGGCTTGTTTGTGTACCTCTTTGGAGTTTCGGATATCATAACTCATCAACAAACAAGGTGGTTGAGAATAAGAAAGTCACTATGGGTGGAGGAATGCTAGGGGGGACTGCTGGAACTCGAACTCGAATTTTTCAGAGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

47.3

Weight (kDa)

9.73

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016789)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21200
malus_domestica MD03G1217100.v1.1 MD11G1234800.v1.1
prunus_persica Prupe.4G192300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0036261
rosa_laevigata RLG00000033682
rosa_multiflora Rmu_sc0000247.1_g000012
rosa_roxburghii Rroxscaffold_1G00044530
rosa_rugosa Rorug05G0154400
rosa_samantha Rh5AG248100 Rh5BG248700 Rh5CG280000 Rh5DG257000
rosa_wichuraiana Rw5G022750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AciI CCGC 2 cut(s) 21, 173
AclWI GGATC 1 cut(s) 551
AcsI RAATTY 2 cut(s) 894, 1263
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 1 cut(s) 1152
AfeI AGCGCT 1 cut(s) 100
AfiI CCNNNNNNNGG 2 cut(s) 75, 876
AflIII ACRYGT 1 cut(s) 527
AjiI CACGTC 1 cut(s) 1006
AjnI CCWGG 1 cut(s) 705
AluBI AGCT 2 cut(s) 1055, 1111
AluI AGCT 2 cut(s) 1055, 1111
Alw21I GWGCWC 2 cut(s) 44, 1130
Alw26I GTCTC 2 cut(s) 123, 590
Alw44I GTGCAC 2 cut(s) 40, 1126
AlwI GGATC 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 808
Aor51HI AGCGCT 1 cut(s) 100
AoxI GGCC 3 cut(s) 66, 582, 1060
ApaLI GTGCAC 2 cut(s) 40, 1126
ApoI RAATTY 2 cut(s) 894, 1263
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
AseI ATTAAT 1 cut(s) 957
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 67, 1060
AsuHPI GGTGA 1 cut(s) 415
AsuII TTCGAA 1 cut(s) 9
AvaI CYCGRG 1 cut(s) 808
BaeGI GKGCMC 2 cut(s) 44, 1130
BanI GGYRCC 1 cut(s) 154
BbsI GAAGAC 3 cut(s) 98, 714, 736
Bbv12I GWGCWC 2 cut(s) 44, 1130
BceAI ACGGC 2 cut(s) 266, 569
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 1 cut(s) 707
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 2 cut(s) 123, 590
BfaI CTAG 4 cut(s) 609, 669, 1085, 1235
BfoI RGCGCY 1 cut(s) 102
BglI GCCNNNNNGGC 1 cut(s) 250
Bme1390I CCNGG 1 cut(s) 707
BmeT110I CYCGRG 1 cut(s) 808
BmgBI CACGTC 1 cut(s) 1006
BmgT120I GGNCC 2 cut(s) 67, 1060
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 1 cut(s) 707
BmsI GCATC 2 cut(s) 150, 486
BpiI GAAGAC 3 cut(s) 98, 714, 736
BpmI CTGGAG 1 cut(s) 696
Bpu14I TTCGAA 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 49, 186, 706
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 876
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 609, 679, 1021
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 3 cut(s) 49, 186, 706
BseGI GGATG 3 cut(s) 59, 182, 217
BseLI CCNNNNNNNGG 2 cut(s) 75, 876
BseNI ACTGG 3 cut(s) 609, 679, 1021
BseSI GKGCMC 2 cut(s) 44, 1130
BshFI GGCC 3 cut(s) 68, 584, 1062
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 44, 1130
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 1 cut(s) 170
BslFI GGGAC 2 cut(s) 395, 1255
BslI CCNNNNNNNGG 2 cut(s) 75, 876
BsmAI GTCTC 2 cut(s) 123, 590
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 395, 1255
BsmI GAATGC 1 cut(s) 1236
BsnI GGCC 3 cut(s) 68, 584, 1062
BsoBI CYCGRG 1 cut(s) 808
Bsp119I TTCGAA 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 44, 1130
Bsp143I GATC 6 cut(s) 397, 420, 535, 543, 842, 899
Bsp19I CCATGG 2 cut(s) 49, 186
BspACI CCGC 2 cut(s) 21, 173
BspANI GGCC 3 cut(s) 68, 584, 1062
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 1 cut(s) 551
BspQI GCTCTTC 1 cut(s) 972
BspT104I TTCGAA 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 3 cut(s) 609, 679, 1021
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 49, 186, 706
BssMI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
BssT1I CCWWGG 2 cut(s) 49, 186
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 2 cut(s) 451, 787
Bst6I CTCTTC 3 cut(s) 471, 742, 972
BstBI TTCGAA 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 40, 171
BstDSI CCRYGG 2 cut(s) 49, 186
BstF5I GGATG 3 cut(s) 59, 182, 217
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 6 cut(s) 400, 423, 538, 546, 845, 902
BstMAI GTCTC 2 cut(s) 123, 590
BstMBI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
BstMWI GCNNNNNNNGC 2 cut(s) 44, 250
BstNI CCWGG 1 cut(s) 707
BstNSI RCATGY 2 cut(s) 531, 989
BstSCI CCNGG 1 cut(s) 705
BstSLI GKGCMC 2 cut(s) 44, 1130
BstV2I GAAGAC 3 cut(s) 98, 714, 736
BsuRI GGCC 3 cut(s) 68, 584, 1062
BtgI CCRYGG 2 cut(s) 49, 186
BtrI CACGTC 1 cut(s) 1006
BtsCI GGATG 3 cut(s) 59, 182, 217
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 5 cut(s) 42, 602, 672, 792, 1014
Cac8I GCNNGC 2 cut(s) 40, 171
CciI TCATGA 1 cut(s) 700
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 67, 1060
CseI GACGC 1 cut(s) 708
Csp6I GTAC 1 cut(s) 1151
CspCI CAANNNNNGTGG 2 cut(s) 1176, 1211
CviAII CATG 7 cut(s) 50, 187, 254, 266, 528, 701, 986
CviQI GTAC 1 cut(s) 1151
DpnI GATC 6 cut(s) 399, 422, 537, 545, 844, 901
DpnII GATC 6 cut(s) 397, 420, 535, 543, 842, 899
DraI TTTAAA 1 cut(s) 579
Eam1104I CTCTTC 3 cut(s) 471, 742, 972
EarI CTCTTC 3 cut(s) 471, 742, 972
Eco130I CCWWGG 2 cut(s) 49, 186
Eco32I GATATC 1 cut(s) 1172
Eco47III AGCGCT 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 808
EcoRII CCWGG 1 cut(s) 705
EcoRV GATATC 1 cut(s) 1172
EcoT14I CCWWGG 2 cut(s) 49, 186
ErhI CCWWGG 2 cut(s) 49, 186
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FaqI GGGAC 2 cut(s) 395, 1255
FatI CATG 7 cut(s) 49, 186, 253, 265, 527, 700, 985
FbaI TGATCA 1 cut(s) 420
FokI GGATG 3 cut(s) 66, 169, 224
FspBI CTAG 4 cut(s) 609, 669, 1085, 1235
GlaI GCGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 696
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 3 cut(s) 68, 584, 1062
HapII CCGG 1 cut(s) 170
HgaI GACGC 1 cut(s) 708
HhaI GCGC 1 cut(s) 101
Hin1II CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 1109
HinfI GANTC 9 cut(s) 258, 310, 616, 647, 680, 828, 950, 1081, 1273
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 4 cut(s) 42, 729, 1128, 1151
Hpy188I TCNGA 9 cut(s) 199, 328, 397, 492, 823, 842, 889, 1169, 1272
Hpy188III TCNNGA 7 cut(s) 289, 304, 386, 533, 595, 701, 930
Hpy8I GTNNAC 4 cut(s) 42, 729, 1128, 1151
Hpy99I CGWCG 2 cut(s) 496, 561
HpyAV CCTTC 3 cut(s) 38, 466, 510
HpyCH4III ACNGT 2 cut(s) 451, 787
HpyCH4IV ACGT 3 cut(s) 462, 559, 1005
HpyCH4V TGCA 6 cut(s) 42, 62, 225, 341, 989, 1128
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 250
HpySE526I ACGT 3 cut(s) 462, 559, 1005
Hsp92II CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
HspAI GCGC 1 cut(s) 99
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Ksp22I TGATCA 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 6 cut(s) 397, 420, 535, 543, 842, 899
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 1 cut(s) 800
LweI GCATC 2 cut(s) 150, 486
MaeI CTAG 4 cut(s) 609, 669, 1085, 1235
MaeII ACGT 3 cut(s) 462, 559, 1005
MaeIII GTNAC 3 cut(s) 789, 1006, 1213
MalI GATC 6 cut(s) 399, 422, 537, 545, 844, 901
MboI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
MhlI GDGCHC 2 cut(s) 44, 1130
MluCI AATT 7 cut(s) 279, 366, 871, 894, 925, 958, 1263
MlyI GAGTC 1 cut(s) 1090
MmeI TCCRAC 5 cut(s) 374, 515, 912, 927, 933
MnlI CCTC 9 cut(s) 42, 69, 472, 682, 804, 870, 1031, 1164, 1220
MroNI GCCGGC 1 cut(s) 169
MseI TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
MslI CAYNNNNRTG 3 cut(s) 405, 996, 1133
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 1236
MvaI CCWGG 1 cut(s) 707
MwoI GCNNNNNNNGC 2 cut(s) 44, 250
NaeI GCCGGC 1 cut(s) 171
NcoI CCATGG 2 cut(s) 49, 186
NdeII GATC 6 cut(s) 397, 420, 535, 543, 842, 899
NgoMIV GCCGGC 1 cut(s) 169
NlaIII CATG 7 cut(s) 53, 190, 257, 269, 531, 704, 989
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 3 cut(s) 789, 1006, 1213
NspI RCATGY 2 cut(s) 531, 989
NspV TTCGAA 1 cut(s) 9
PaeR7I CTCGAG 1 cut(s) 808
PagI TCATGA 1 cut(s) 700
PciI ACATGT 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 972
PctI GAATGC 1 cut(s) 1236
PdiI GCCGGC 1 cut(s) 171
PfeI GAWTC 7 cut(s) 258, 310, 616, 647, 680, 828, 950
PleI GAGTC 1 cut(s) 1089
PpsI GAGTC 1 cut(s) 1089
PscI ACATGT 1 cut(s) 527
PshBI ATTAAT 1 cut(s) 957
Psp6I CCWGG 1 cut(s) 705
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 67, 1060
PspXI VCTCGAGB 1 cut(s) 808
RsaI GTAC 1 cut(s) 1152
RsaNI GTAC 1 cut(s) 1151
RseI CAYNNNNRTG 3 cut(s) 405, 996, 1133
SapI GCTCTTC 1 cut(s) 972
SaqAI TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
Sau3AI GATC 6 cut(s) 397, 420, 535, 543, 842, 899
Sau96I GGNCC 2 cut(s) 67, 1060
SchI GAGTC 1 cut(s) 1090
ScrFI CCNGG 1 cut(s) 707
SduI GDGCHC 2 cut(s) 44, 1130
SfaNI GCATC 2 cut(s) 150, 486
Sfr274I CTCGAG 1 cut(s) 808
SfuI TTCGAA 1 cut(s) 9
SlaI CTCGAG 1 cut(s) 808
SmiMI CAYNNNNRTG 3 cut(s) 405, 996, 1133
SmlI CTYRAG 2 cut(s) 384, 808
SmoI CTYRAG 2 cut(s) 384, 808
Sse9I AATT 7 cut(s) 279, 366, 871, 894, 925, 958, 1263
SsiI CCGC 2 cut(s) 21, 173
SspI AATATT 1 cut(s) 346
SspMI CTAG 4 cut(s) 609, 669, 1085, 1235
StyD4I CCNGG 1 cut(s) 705
StyI CCWWGG 2 cut(s) 49, 186
TaaI ACNGT 2 cut(s) 451, 787
TaiI ACGT 3 cut(s) 465, 562, 1008
TaqI TCGA 8 cut(s) 9, 594, 809, 898, 929, 962, 1255, 1261
TasI AATT 7 cut(s) 279, 366, 871, 894, 925, 958, 1263
TfiI GAWTC 7 cut(s) 258, 310, 616, 647, 680, 828, 950
Tru1I TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
Tru9I TTAA 6 cut(s) 513, 578, 662, 728, 957, 996
TscAI CASTG 5 cut(s) 49, 609, 679, 792, 1021
TseFI GTSAC 3 cut(s) 789, 1006, 1213
Tsp45I GTSAC 3 cut(s) 789, 1006, 1213
TspDTI ATGAA 5 cut(s) 254, 492, 534, 741, 768
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 5 cut(s) 49, 609, 679, 792, 1021
VneI GTGCAC 2 cut(s) 40, 1126
VspI ATTAAT 1 cut(s) 957
XapI RAATTY 2 cut(s) 894, 1263
XceI RCATGY 2 cut(s) 531, 989
XhoI CTCGAG 1 cut(s) 808
XspI CTAG 4 cut(s) 609, 669, 1085, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.