MD03G1225500.v1.1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008363
Physical Location & Seq
Reverse (-)
31102744 .. 31109341
6598 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1225500.v1.1.491

Sequence Viewer

Length: 2121 bp
ATGAGGGGTCCTATGGGGGCAGTGATAGGGAGGTACCCACCAAGTGATGGGAGTGCCCAAATGGGTTCAATCATTAGGCACAACAGGAAATGCAGGGACCTTGTCTTCCTCGTAATCTTCATAGCCTTCTGGGTTGCCATGATTGTTAACTCCAGCTTTGGATTCAACCAAGGAAACCCTTTAAGGCTCACTTATGGACTGGACTACAAAGGAAATGTTTGTGGTGACAAGCATGCAAAACCTGGTCTTCATGAACTGGAACTCAAATATTGGTTAAATCCTAACCAGGTTTACCTAAGTGGTTTAAAAGACAATCAATTTAAATTGGCTAATGCTCGGAGTATATGCTTAATGGATTGCCCTACCCCATCTGTAGATTCATTAAGTTGGGTGTGTGATTATCCTGATGGAGATATCCGTCTCAAAATGGGTGATTGGATCGACAGGAATTATGATTATTATGAGTTCCTTACACCAGAAATGAGAAACACCTCTCTTCAACTTCAGGGTCCTTGCTACCCAGTCATATTTCCCAGTGTAAATGTTTATTGGACCTGCCAATTTATTGCTCGTCCCTCCAACGTATCTTTGAGGCATTGGCAGCAAATGGGTGGAGTGAATATCAACGAGGACATTATTATAGACAAATCTATTCACAAGGCCATCAATTCTCGGGCATCTGTCTTTAAGAGATACATGGCTGATATTGGAAAGGCATGGCCAGTGCTGATTGTTTGTGGAGGAATATTGCCACTTTTTCTGTCTGTGATTTGGCTGCTTCTTATTCGTCATTTTGTTGCTGCAATGCCTTGGATTACAGTCGGCCTCTTTAATATTCTCATGATAGCAGTCACAATGTTCTATTACTTGAAAGCTGGATGGATAGGAAATGATGGCATCTCCCCAATTATTGGTGAGCACGATCCATACATCCGCATAACTGGAAGGGAGTTACATCATATCCGTGTTGTTGCTGTTATTATGACCTTTATTATGGTTGTTGCTGTTCTTACATCAATAGCCATAGTTCGACGCATCCTTATGGCAACATCTGTTCTTAAGGTTGCTGCAAAGGTCATAGGAGAAGCTCAGGCGCTCATAATATTTCCAGTCATTCCATATACCATCCTTGCAATTTTCTACATGTTCTGGTTTTCAGCTGCTTTCCATCTGTTAAGTTCTGGGCAGGTAGTCCAGAATGACTGCAATTTGAACTGCTGTGCTTATGATCTTGTATCAAAACGAGTCAACTGTGATCATTGTTGCGGTTATAGCATTCATTACACCCCTCATATTGGAGTTTCCATCCTTTTCCACCTATTTGGGGGATATTGGGCTACCCAGTTTTTTATAGCATGCTCTTCCACAGTTATTGCGGGTTCTGTTGCCTCTTATTATTGGGCTCGTGGTGAAACTTCACCGGAAATTCCATTTCTTCCAGTCTTTGCCTCCATGAGAAGGCTTGCTCGCTATAACCTTGGGTCGGTTGCTCTCGGCTCCCTGATTGTGTCATTTGTGGAATCAATCCGCTTTATGCTTGAGTCAATTCGTCGCAGAATAAAAGTTGGTGGAACCACACCCAATAACTGGTTCGGAAGGGCAGTGTACCATACTTCTAGATTTTGCCTGATGTGTATTGAGTGGACAATCAGATCAGCCAATCGCAATGCCTACATTATGATTGCCATAACAGGTAAAAGCTTCTGCAACGCTTCTGCAATCGCAACAGATTTGATCCTTAATAACATTCTTCGGATAGGGAGGGTGAATGTAATTGGAGATGTTATCCTATTCCTTGGAAAATTATGTGTCAGCCTCTCAACAGCCCTCTTTGCTTTCCTGATGTTGGATACCCACAAATACAAATCTGCCCACAACAAGATCACTTCTCCACTGTTTCCCGTGTTGGTTTGCTGGGGTCTTGGTTATGTGGTTGCCACTCTTTTCTTTGGAGTGGTGGAGATGTCAATCGATACCATCATTCTTTCATACTGCCAGGATTCTGAAGAGCACCAAGGGACTGCCCAATATGCTCCGCCACTTCTGATCGAAACTCTAAACGACCAAAACGAGATGCAGAGACTCACCCAAGGACCCCAATCAAACAGCCTAGATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000139 GO:0000325 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0005215 GO:0005326 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006836 GO:0007275 GO:0007389 GO:0008104 GO:0008150 GO:0008324 GO:0008519 GO:0009505 GO:0009506 GO:0009524 GO:0009551 GO:0009663 GO:0009705 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010008 GO:0010051 GO:0010065 GO:0010067 GO:0010087 GO:0010088 GO:0010154 GO:0010496 GO:0010497 GO:0010817 GO:0012505 GO:0015031 GO:0015075 GO:0015101 GO:0015220 GO:0015695 GO:0015696 GO:0015833 GO:0015871 GO:0016020 GO:0016043 GO:0016192 GO:0022414 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0030054 GO:0030100 GO:0030154 GO:0030312 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0033036 GO:0034220 GO:0034330 GO:0040008 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044426 GO:0044431 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044462 GO:0044464 GO:0045184 GO:0045216 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051510 GO:0055044 GO:0055085 GO:0055088 GO:0060627 GO:0061458 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072488 GO:0090603 GO:0097218 GO:0097708 GO:0098588 GO:0098655 GO:0098791 GO:0098805 GO:0099402 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko05231 map05231
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

707

Amino Acids

79.3

Weight (kDa)

8.79

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 326 - 669 4.2e-88 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 563, 1177
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 1 cut(s) 33
AccB7I CCANNNNNTGG 3 cut(s) 47, 911, 1587
AciI CCGC 5 cut(s) 934, 1266, 1376, 1528, 2036
AclWI GGATC 3 cut(s) 446, 917, 1729
AcoI YGGCCR 1 cut(s) 719
AcsI RAATTY 1 cut(s) 1425
AcuI CTGAAG 2 cut(s) 488, 2025
AdeI CACNNNGTG 1 cut(s) 44
AfaI GTAC 2 cut(s) 35, 1607
AfiI CCNNNNNNNGG 8 cut(s) 47, 911, 1295, 1324, 1458, 1483, 1587, 1846
AflII CTTAAG 1 cut(s) 1058
AflIII ACRYGT 1 cut(s) 1143
AgsI TTSAA 5 cut(s) 69, 166, 500, 871, 1213
AjnI CCWGG 3 cut(s) 241, 285, 1995
AjuI GAANNNNNNNTTGG 2 cut(s) 1574, 1606
AluBI AGCT 5 cut(s) 156, 875, 1088, 1160, 1701
AluI AGCT 5 cut(s) 156, 875, 1088, 1160, 1701
Alw21I GWGCWC 2 cut(s) 921, 2013
Alw26I GTCTC 2 cut(s) 425, 2074
AlwI GGATC 3 cut(s) 446, 917, 1729
Ama87I CYCGRG 1 cut(s) 672
AoxI GGCC 3 cut(s) 660, 719, 823
ApeKI GCWGC 5 cut(s) 601, 775, 800, 1067, 1160
ApoI RAATTY 1 cut(s) 1425
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 1 cut(s) 1096
AspS9I GGNCC 5 cut(s) 8, 97, 509, 552, 2093
AsuHPI GGTGA 7 cut(s) 236, 443, 926, 1410, 1421, 1777, 2077
AvaI CYCGRG 1 cut(s) 672
AvaII GGWCC 5 cut(s) 8, 97, 509, 552, 2093
BaeGI GKGCMC 1 cut(s) 58
BalI TGGCCA 1 cut(s) 721
BanI GGYRCC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 1405
BauI CACGAG 1 cut(s) 1404
BbsI GAAGAC 2 cut(s) 97, 239
Bbv12I GWGCWC 2 cut(s) 921, 2013
BbvI GCAGC 5 cut(s) 613, 762, 787, 1054, 1147
BciT130I CCWGG 3 cut(s) 243, 287, 1997
BciVI GTATCC 1 cut(s) 1843
BclI TGATCA 1 cut(s) 1255
BcoDI GTCTC 2 cut(s) 425, 2074
BfaI CTAG 2 cut(s) 1617, 2111
BfmI CTRYAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 1097
BfrI CTTAAG 1 cut(s) 1058
BfuAI ACCTGC 2 cut(s) 563, 1177
BfuI GTATCC 1 cut(s) 1843
BisI GCNGC 5 cut(s) 602, 776, 801, 1068, 1161
BlsI GCNGC 5 cut(s) 603, 777, 802, 1069, 1162
Bme1390I CCNGG 3 cut(s) 243, 287, 1997
Bme18I GGWCC 5 cut(s) 8, 97, 509, 552, 2093
BmeT110I CYCGRG 1 cut(s) 672
BmgT120I GGNCC 5 cut(s) 8, 97, 509, 552, 2093
BmiI GGNNCC 7 cut(s) 9, 35, 98, 510, 1498, 1573, 2095
BmrFI CCNGG 3 cut(s) 243, 287, 1997
BmrI ACTGGG 3 cut(s) 515, 528, 1336
BmsI GCATC 4 cut(s) 686, 906, 1044, 2064
BmuI ACTGGG 3 cut(s) 515, 528, 1336
BpiI GAAGAC 2 cut(s) 97, 239
BpmI CTGGAG 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 1089
BpuEI CTTGAG 1 cut(s) 1559
Bsa29I ATCGAT 1 cut(s) 1971
BsaBI GATNNNNATC 1 cut(s) 1967
BsaJI CCNNGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
BsaWI WCCGGW 1 cut(s) 1420
Bsc4I CCNNNNNNNGG 8 cut(s) 47, 911, 1295, 1324, 1458, 1483, 1587, 1846
Bse3DI GCAATG 2 cut(s) 810, 1672
Bse8I GATNNNNATC 1 cut(s) 1967
BseBI CCWGG 3 cut(s) 243, 287, 1997
BseCI ATCGAT 1 cut(s) 1971
BseDI CCNNGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
BseGI GGATG 5 cut(s) 884, 930, 1035, 1125, 1305
BseJI GATNNNNATC 1 cut(s) 1967
BseLI CCNNNNNNNGG 8 cut(s) 47, 911, 1295, 1324, 1458, 1483, 1587, 1846
BseMI GCAATG 2 cut(s) 810, 1672
BseMII CTCAG 1 cut(s) 1103
BseSI GKGCMC 1 cut(s) 58
BseXI GCAGC 5 cut(s) 613, 762, 787, 1054, 1147
BseYI CCCAGC 1 cut(s) 1914
BshFI GGCC 3 cut(s) 662, 721, 825
BshNI GGYRCC 1 cut(s) 33
BshVI ATCGAT 1 cut(s) 1971
BsiHKAI GWGCWC 2 cut(s) 921, 2013
BsiHKCI CYCGRG 1 cut(s) 672
BsiSI CCGG 1 cut(s) 1421
BslFI GGGAC 3 cut(s) 110, 558, 2032
BslI CCNNNNNNNGG 8 cut(s) 47, 911, 1295, 1324, 1458, 1483, 1587, 1846
BsmAI GTCTC 2 cut(s) 425, 2074
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 3 cut(s) 110, 558, 2032
BsmI GAATGC 1 cut(s) 1275
BsnI GGCC 3 cut(s) 662, 721, 825
BsoBI CYCGRG 1 cut(s) 672
Bsp1286I GDGCHC 4 cut(s) 58, 921, 1405, 2013
Bsp143I GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
BspACI CCGC 5 cut(s) 934, 1266, 1376, 1528, 2036
BspANI GGCC 3 cut(s) 662, 721, 825
BspCNI CTCAG 1 cut(s) 1102
BspDI ATCGAT 1 cut(s) 1971
BspHI TCATGA 2 cut(s) 250, 840
BspLI GGNNCC 7 cut(s) 9, 35, 98, 510, 1498, 1573, 2095
BspMI ACCTGC 2 cut(s) 563, 1177
BspPI GGATC 3 cut(s) 446, 917, 1729
BspQI GCTCTTC 2 cut(s) 1366, 2001
BspT107I GGYRCC 1 cut(s) 33
BspTI CTTAAG 1 cut(s) 1058
BsrDI GCAATG 2 cut(s) 810, 1672
BssECI CCNNGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
BssMI GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
BssSI CACGAG 1 cut(s) 1404
BssT1I CCWWGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
Bst2BI CACGAG 1 cut(s) 1404
Bst2UI CCWGG 3 cut(s) 243, 287, 1997
Bst4CI ACNGT 4 cut(s) 820, 1253, 1369, 1896
Bst6I CTCTTC 3 cut(s) 501, 1366, 2001
BstAFI CTTAAG 1 cut(s) 1058
BstC8I GCNNGC 4 cut(s) 234, 1357, 1464, 1468
BstDEI CTNAG 2 cut(s) 296, 1089
BstF5I GGATG 5 cut(s) 884, 930, 1035, 1125, 1305
BstH2I RGCGCY 1 cut(s) 1097
BstHHI GCGC 1 cut(s) 1096
BstKTI GATC 8 cut(s) 441, 925, 1231, 1258, 1655, 1737, 1884, 2049
BstMAI GTCTC 2 cut(s) 425, 2074
BstMBI GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
BstMWI GCNNNNNNNGC 4 cut(s) 601, 1272, 1832, 2030
BstNI CCWGG 3 cut(s) 243, 287, 1997
BstNSI RCATGY 3 cut(s) 236, 1147, 1359
BstSCI CCNGG 3 cut(s) 241, 285, 1995
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 58
BstV1I GCAGC 5 cut(s) 613, 762, 787, 1054, 1147
BstV2I GAAGAC 2 cut(s) 97, 239
BstXI CCANNNNNNTGG 1 cut(s) 1322
Bsu15I ATCGAT 1 cut(s) 1971
BsuI GTATCC 1 cut(s) 1843
BsuRI GGCC 3 cut(s) 662, 721, 825
BsuTUI ATCGAT 1 cut(s) 1971
BtsCI GGATG 5 cut(s) 884, 930, 1035, 1125, 1305
BtsI GCAGTG 2 cut(s) 27, 1608
BtsIMutI CAGTG 5 cut(s) 27, 541, 729, 1608, 1892
BveI ACCTGC 2 cut(s) 563, 1177
Cac8I GCNNGC 4 cut(s) 234, 1357, 1464, 1468
CciI TCATGA 2 cut(s) 250, 840
CfoI GCGC 1 cut(s) 1096
Cfr13I GGNCC 5 cut(s) 8, 97, 509, 552, 2093
ClaI ATCGAT 1 cut(s) 1971
CseI GACGC 1 cut(s) 1041
CsiI ACCWGGT 2 cut(s) 241, 285
Csp6I GTAC 2 cut(s) 34, 1606
CspCI CAANNNNNGTGG 2 cut(s) 1354, 1389
CviAII CATG 9 cut(s) 139, 233, 251, 697, 717, 841, 1144, 1356, 1453
CviQI GTAC 2 cut(s) 34, 1606
DdeI CTNAG 2 cut(s) 296, 1089
DpnI GATC 8 cut(s) 440, 924, 1230, 1257, 1654, 1736, 1883, 2048
DpnII GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
DraI TTTAAA 2 cut(s) 306, 322
DraIII CACNNNGTG 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 719
Eam1104I CTCTTC 3 cut(s) 501, 1366, 2001
EarI CTCTTC 3 cut(s) 501, 1366, 2001
EciI GGCGGA 1 cut(s) 2025
Eco130I CCWWGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
Eco24I GRGCYC 1 cut(s) 1405
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 5 cut(s) 8, 97, 509, 552, 2093
Eco57I CTGAAG 2 cut(s) 488, 2025
Eco88I CYCGRG 1 cut(s) 672
EcoO109I RGGNCCY 4 cut(s) 8, 97, 509, 2093
EcoRII CCWGG 3 cut(s) 241, 285, 1995
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
EcoT38I GRGCYC 1 cut(s) 1405
ErhI CCWWGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 9 cut(s) 142, 236, 254, 700, 720, 844, 1147, 1359, 1456
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 3 cut(s) 110, 558, 2032
FatI CATG 9 cut(s) 138, 232, 250, 696, 716, 840, 1143, 1355, 1452
FauI CCCGC 1 cut(s) 1369
FbaI TGATCA 1 cut(s) 1255
Fnu4HI GCNGC 5 cut(s) 602, 776, 801, 1068, 1161
FokI GGATG 5 cut(s) 891, 917, 1022, 1112, 1292
FriOI GRGCYC 1 cut(s) 1405
Fsp4HI GCNGC 5 cut(s) 602, 776, 801, 1068, 1161
FspBI CTAG 2 cut(s) 1617, 2111
GlaI GCGC 1 cut(s) 1095
GluI GCNGC 5 cut(s) 602, 776, 801, 1068, 1161
GsaI CCCAGC 1 cut(s) 1918
GsuI CTGGAG 1 cut(s) 136
HaeII RGCGCY 1 cut(s) 1097
HaeIII GGCC 3 cut(s) 662, 721, 825
HapII CCGG 1 cut(s) 1421
HgaI GACGC 1 cut(s) 1041
HhaI GCGC 1 cut(s) 1096
Hin1II CATG 9 cut(s) 142, 236, 254, 700, 720, 844, 1147, 1359, 1456
Hin6I GCGC 1 cut(s) 1094
HinP1I GCGC 1 cut(s) 1094
HincII GTYRAC 2 cut(s) 148, 1249
HindII GTYRAC 2 cut(s) 148, 1249
HindIII AAGCTT 1 cut(s) 1699
HinfI GANTC 7 cut(s) 162, 377, 1245, 1520, 1541, 2000, 2082
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 1 cut(s) 1421
HphI GGTGA 7 cut(s) 236, 443, 926, 1410, 1421, 1777, 2077
Hpy166II GTNNAC 5 cut(s) 148, 292, 1249, 1606, 1644
Hpy188I TCNGA 6 cut(s) 339, 1595, 1652, 1755, 2005, 2046
Hpy188III TCNNGA 6 cut(s) 251, 404, 841, 1195, 1617, 1840
Hpy8I GTNNAC 5 cut(s) 148, 292, 1249, 1606, 1644
Hpy99I CGWCG 2 cut(s) 1035, 1554
HpyAV CCTTC 4 cut(s) 136, 939, 1452, 1590
HpyCH4III ACNGT 4 cut(s) 820, 1253, 1369, 1896
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 9 cut(s) 93, 236, 803, 1070, 1133, 1206, 1707, 1718, 2077
HpyF10VI GCNNNNNNNGC 4 cut(s) 601, 1272, 1832, 2030
HpyF3I CTNAG 2 cut(s) 296, 1089
HpySE526I ACGT 1 cut(s) 582
Hsp92II CATG 9 cut(s) 142, 236, 254, 700, 720, 844, 1147, 1359, 1456
HspAI GCGC 1 cut(s) 1094
KpnI GGTACC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 1255
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
LguI GCTCTTC 2 cut(s) 1366, 2001
LmnI GCTCC 2 cut(s) 1502, 2038
Lsp1109I GCAGC 5 cut(s) 613, 762, 787, 1054, 1147
LweI GCATC 4 cut(s) 686, 906, 1044, 2064
MabI ACCWGGT 2 cut(s) 241, 285
MaeI CTAG 2 cut(s) 1617, 2111
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 3 cut(s) 224, 850, 951
MalI GATC 8 cut(s) 440, 924, 1230, 1257, 1654, 1736, 1883, 2048
MboI GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
MboII GAAGA 8 cut(s) 97, 109, 239, 488, 1353, 1427, 1742, 2018
MhlI GDGCHC 4 cut(s) 58, 921, 1405, 2013
MlsI TGGCCA 1 cut(s) 721
MluNI TGGCCA 1 cut(s) 721
MlyI GAGTC 3 cut(s) 1254, 1550, 2076
MmeI TCCRAC 2 cut(s) 603, 1827
Mox20I TGGCCA 1 cut(s) 721
MscI TGGCCA 1 cut(s) 721
MslI CAYNNNNRTG 2 cut(s) 963, 1040
Msp20I TGGCCA 1 cut(s) 721
MspA1I CMGCKG 1 cut(s) 1160
MspCI CTTAAG 1 cut(s) 1058
MspI CCGG 1 cut(s) 1421
MspR9I CCNGG 3 cut(s) 243, 287, 1997
Mva1269I GAATGC 1 cut(s) 1275
MvaI CCWGG 3 cut(s) 243, 287, 1997
MwoI GCNNNNNNNGC 4 cut(s) 601, 1272, 1832, 2030
NdeII GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
NlaIII CATG 9 cut(s) 142, 236, 254, 700, 720, 844, 1147, 1359, 1456
NlaIV GGNNCC 7 cut(s) 9, 35, 98, 510, 1498, 1573, 2095
NmeAIII GCCGAG 1 cut(s) 1473
NmuCI GTSAC 2 cut(s) 224, 850
NspI RCATGY 3 cut(s) 236, 1147, 1359
PaeI GCATGC 2 cut(s) 236, 1359
PagI TCATGA 2 cut(s) 250, 840
PciI ACATGT 1 cut(s) 1143
PciSI GCTCTTC 2 cut(s) 1366, 2001
PcsI WCGNNNNNNNCGW 1 cut(s) 2067
PctI GAATGC 1 cut(s) 1275
PfeI GAWTC 4 cut(s) 162, 377, 1520, 2000
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 3 cut(s) 47, 911, 1587
PkrI GCNGC 5 cut(s) 603, 777, 802, 1069, 1162
PleI GAGTC 3 cut(s) 1253, 1549, 2076
PpsI GAGTC 3 cut(s) 1253, 1549, 2076
PpuMI RGGWCCY 4 cut(s) 8, 97, 509, 2093
PscI ACATGT 1 cut(s) 1143
Psp5II RGGWCCY 4 cut(s) 8, 97, 509, 2093
Psp6I CCWGG 3 cut(s) 241, 285, 1995
PspFI CCCAGC 1 cut(s) 1914
PspGI CCWGG 3 cut(s) 241, 285, 1995
PspN4I GGNNCC 7 cut(s) 9, 35, 98, 510, 1498, 1573, 2095
PspPI GGNCC 5 cut(s) 8, 97, 509, 552, 2093
PspPPI RGGWCCY 4 cut(s) 8, 97, 509, 2093
PsyI GACNNNGTC 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 2 cut(s) 35, 1607
RsaNI GTAC 2 cut(s) 34, 1606
RseI CAYNNNNRTG 2 cut(s) 963, 1040
SapI GCTCTTC 2 cut(s) 1366, 2001
SatI GCNGC 5 cut(s) 602, 776, 801, 1068, 1161
Sau3AI GATC 8 cut(s) 438, 922, 1228, 1255, 1652, 1734, 1881, 2046
Sau96I GGNCC 5 cut(s) 8, 97, 509, 552, 2093
SchI GAGTC 3 cut(s) 1254, 1550, 2076
ScrFI CCNGG 3 cut(s) 243, 287, 1997
SduI GDGCHC 4 cut(s) 58, 921, 1405, 2013
SexAI ACCWGGT 2 cut(s) 241, 285
SfaNI GCATC 4 cut(s) 686, 906, 1044, 2064
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 5 cut(s) 8, 97, 509, 552, 2093
SmiI ATTTAAAT 1 cut(s) 322
SmiMI CAYNNNNRTG 2 cut(s) 963, 1040
SmlI CTYRAG 2 cut(s) 1058, 1538
SmoI CTYRAG 2 cut(s) 1058, 1538
SphI GCATGC 2 cut(s) 236, 1359
SsiI CCGC 5 cut(s) 934, 1266, 1376, 1528, 2036
SspI AATATT 4 cut(s) 269, 747, 835, 1104
SspMI CTAG 2 cut(s) 1617, 2111
StyD4I CCNGG 3 cut(s) 241, 285, 1995
StyI CCWWGG 6 cut(s) 169, 809, 1477, 1795, 2014, 2089
SwaI ATTTAAAT 1 cut(s) 322
TaaI ACNGT 4 cut(s) 820, 1253, 1369, 1896
TaiI ACGT 1 cut(s) 585
TaqI TCGA 4 cut(s) 441, 1030, 1971, 2049
TfiI GAWTC 4 cut(s) 162, 377, 1520, 2000
TscAI CASTG 5 cut(s) 27, 541, 729, 1608, 1899
TseFI GTSAC 2 cut(s) 224, 850
TseI GCWGC 5 cut(s) 601, 775, 800, 1067, 1160
Tsp45I GTSAC 2 cut(s) 224, 850
TspDTI ATGAA 6 cut(s) 109, 239, 267, 369, 1268, 1977
TspGWI ACGGA 2 cut(s) 407, 953
TspRI CASTG 5 cut(s) 27, 541, 729, 1608, 1899
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 3 cut(s) 47, 911, 1587
Vha464I CTTAAG 1 cut(s) 1058
VpaK11BI GGWCC 5 cut(s) 8, 97, 509, 552, 2093
XapI RAATTY 1 cut(s) 1425
XbaI TCTAGA 1 cut(s) 1616
XceI RCATGY 3 cut(s) 236, 1147, 1359
XspI CTAG 2 cut(s) 1617, 2111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.