Rw5G021280

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008363
Physical Location & Seq
Reverse (-)
28547386 .. 28553482
6097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021280.1

Sequence Viewer

Length: 1944 bp
ATGAGGGGTCCTTTGGGGGCAGTGATAGGGAGGTACCCATCAAGTGATGGGAGTGACCAGATGAGCTCAATCATCAGGCACAACAGGAAATGCAAGGACCTTGTGTTCCTGGTCATCTTTATAGCCTTCTGGGTTGCTATGATTGTCAACTCAAGCTTTGGATTCAATCAAGGAAACCCATTAAGGCTCACTTATGGACTGGACTACAAAGGAAATGTTTGTGGCGACAGGCATGCAAAGCCTGGTCTTCGGGAACTCGAACTCAAATATTGGTTAAATCCAAACCAGGTTTACCTGAGTGGTCTAAAAGACAATCAGTTCGACCTGGCTGATGCCCGGAGTATATGCCTATTGGATTGCCCCGCCCCTTCTGAAGATACACTGAATTGGGTGTGTGATTACCCTGAAGGAGAGATCCGTCTTTCAATGAATGATTGGATTGACAGGAATTATGATTATTTTGAGTTCCTTACGCCTGAAATGCGAAATGCCTCTCTTCAACTCGGGGTCCTTGTTACCCTCTATTGGAGCTGCCAATTTATTGCCCGTCCATCAAACAATTCGTTGAAGCATTGGCAGCAAATGGACAAATCTATTCACAGTTCCATCAATTCTCGGTCAGCTGTCTTAAAGAGATACATGGCCGATATTGGAAAGTCATGGCGAGTGCTGCTTGTTTGTGGAGGAATCGTGCCACTGGCTTTGTCATTGATTTGGCTGCTTCTGATTCGTCATTTTGTTGCTGCAATGCCTTGGATAACAGTGGCCCTCTTTAATATTCTCATAATATTAGTCACAATGTTTTACTACTTGAAAGCTGGATGGATAGGAAATGATGCCATCTCCCCAATCATGGAGTTGCATCATCTCCGTGCTGTTGCAGTTCTCATGACCTTTATTATGGTTGTTTCTGTTCTTACATCAATTGCTATAGTTCGCCGCATCCTTATGGCAACATCTGTCCTTAAGGCAAGTTGCTGCCAAGGTCATAGGAGAAGCTCAAGCACTCATAATATTTCCAATAATTCCATATACCATCCTTGGAGTTTTTACATGATCTGGTTTTCAGCTGCTTTTCACCTGTTCAGTTCTGGTCAAGTAGTCCAGAATAGCTGCAACTCGAACTGCTGTGCCTATGATCTTGCATCAAAACAAGTTCACTGTGATCGTTGTTGCGGTTATAGCATTCATTACACTCCTCATATTGGAATTGCTATCCTTTTACTATTGGTTTTATTTGGTTATGTGCCATTTTTATCTTACTCATTATTTTTTGTAATGCAGGGTGAAATACCATTTCTTCCGGTCTTTTCCTCTATGAAACGGCTGATGCGCTATAGCCTCGGGTCGGTGGCTCTTGGCTCCCTGATTGTGTCATTTGTGGAATCAATAAGGTTTATGCTCGAGTCAATTCGTCGCCGGAGTCAATTCGCTGCATTCTCTACTGCTAGGTTTTGCCTGAGCTGTATTGAGTGGACAATCAAGTCGGTTAATCGCAATGCCTACATTATGATTGCTATAACAGGCAAAAGCTTCTGCAAGTCTTCTGCAGTTGCTACAGATCTGATCATCAGTAACATCCTTCGGATAGGGAGGGTGAACGTGATTGGAGATGTGATACTATTCCTTGGCAAATTGTGCGTCAGCCTATCAACTGCCCTTTTTGCTTTTCTTATGTTGGATACCCACAGATACAAATCTGCGCATAACAAGATCTCTTCTCCACTGTTTCCAGTGTTGGTTTGCTGGGCCCTTGGTTATGTGGTTGCTACACTTTTCTTTGCTGTGGTGGAGATGTCAATCGATACGATCATTCTTTCATTCTGCCAGGATTCCGAAGAGCACCAAGGGACTGCGCAGTATGCCCCTCCACTTCTCATCGAAACTCTAAATGACGAAAATGAGATGCAGAGACTTACCCAAGGATCCAATGATATACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000139 GO:0000325 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0005215 GO:0005326 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006836 GO:0007275 GO:0007389 GO:0008104 GO:0008150 GO:0008324 GO:0008519 GO:0009505 GO:0009506 GO:0009524 GO:0009551 GO:0009663 GO:0009705 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010008 GO:0010051 GO:0010065 GO:0010067 GO:0010087 GO:0010088 GO:0010154 GO:0010496 GO:0010497 GO:0010817 GO:0012505 GO:0015031 GO:0015075 GO:0015101 GO:0015220 GO:0015695 GO:0015696 GO:0015833 GO:0015871 GO:0016020 GO:0016043 GO:0016192 GO:0022414 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0030054 GO:0030100 GO:0030154 GO:0030312 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0033036 GO:0034220 GO:0034330 GO:0040008 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044426 GO:0044431 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044462 GO:0044464 GO:0045184 GO:0045216 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051510 GO:0055044 GO:0055085 GO:0055088 GO:0060627 GO:0061458 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072488 GO:0090603 GO:0097218 GO:0097708 GO:0098588 GO:0098655 GO:0098791 GO:0098805 GO:0099402 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko05231 map05231
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

647

Amino Acids

73.0

Weight (kDa)

8.7

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 296 - 613 3.6e-58 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1483
Acc16I TGCGCA 2 cut(s) 1704, 1857
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 1 cut(s) 33
AciI CCGC 3 cut(s) 363, 940, 1176
AclWI GGATC 3 cut(s) 409, 1920, 1933
AcoI YGGCCR 1 cut(s) 642
AcuI CTGAAG 2 cut(s) 393, 426
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 4 cut(s) 525, 853, 1205, 1348
AflII CTTAAG 1 cut(s) 965
AgsI TTSAA 5 cut(s) 166, 426, 500, 568, 814
AjnI CCWGG 5 cut(s) 108, 241, 285, 324, 1827
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
Alw21I GWGCWC 2 cut(s) 68, 1845
Alw26I GTCTC 1 cut(s) 1906
AlwI GGATC 3 cut(s) 409, 1920, 1933
Ama87I CYCGRG 3 cut(s) 503, 1343, 1403
AoxI GGCC 3 cut(s) 642, 765, 1749
ApaI GGGCCC 1 cut(s) 1753
ApeKI GCWGC 9 cut(s) 531, 577, 670, 718, 743, 978, 1070, 1113, 1433
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 1626, 1658
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 3 cut(s) 1335, 1705, 1858
AspS9I GGNCC 6 cut(s) 8, 97, 508, 766, 1749, 1750
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 1070, 1298, 1609
AvaI CYCGRG 3 cut(s) 503, 1343, 1403
AvaII GGWCC 3 cut(s) 8, 97, 508
BaeGI GKGCMC 1 cut(s) 1753
BamHI GGATCC 1 cut(s) 1925
BanI GGYRCC 1 cut(s) 33
BanII GRGCYC 2 cut(s) 68, 1753
BbsI GAAGAC 2 cut(s) 239, 1536
Bbv12I GWGCWC 2 cut(s) 68, 1845
BbvI GCAGC 9 cut(s) 518, 589, 657, 705, 730, 965, 1057, 1100, 1420
BccI CCATC 7 cut(s) 41, 46, 559, 614, 816, 848, 1044
BceAI ACGGC 1 cut(s) 1340
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 5 cut(s) 110, 243, 287, 326, 1829
BciVI GTATCC 1 cut(s) 1675
BclI TGATCA 1 cut(s) 1566
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 1906
BfaI CTAG 1 cut(s) 1449
BfmI CTRYAG 4 cut(s) 930, 1336, 1548, 1557
BfrI CTTAAG 1 cut(s) 965
BfuI GTATCC 1 cut(s) 1675
BglII AGATCT 2 cut(s) 1561, 1713
Bme1390I CCNGG 6 cut(s) 110, 243, 287, 326, 337, 1829
Bme18I GGWCC 3 cut(s) 8, 97, 508
BmeT110I CYCGRG 3 cut(s) 503, 1343, 1403
BmgT120I GGNCC 6 cut(s) 8, 97, 508, 766, 1749, 1750
BmiI GGNNCC 6 cut(s) 9, 35, 509, 1363, 1751, 1927
BmrFI CCNGG 6 cut(s) 110, 243, 287, 326, 337, 1829
BmsI GCATC 7 cut(s) 322, 826, 871, 951, 1154, 1320, 1896
BpiI GAAGAC 2 cut(s) 239, 1536
Bpu10I CCTNAGC 1 cut(s) 1460
BpuEI CTTGAG 2 cut(s) 136, 985
BpuMI CCSGG 1 cut(s) 337
Bsa29I ATCGAT 1 cut(s) 1803
BsaBI GATNNNNATC 2 cut(s) 1696, 1799
BsaJI CCNNGG 8 cut(s) 752, 982, 1040, 1342, 1627, 1753, 1846, 1921
BsaWI WCCGGW 1 cut(s) 1303
BsaXI ACNNNNNCTCC 2 cut(s) 675, 705
Bsc4I CCNNNNNNNGG 4 cut(s) 525, 853, 1205, 1348
Bse1I ACTGG 3 cut(s) 204, 702, 1733
Bse3DI GCAATG 2 cut(s) 753, 1504
Bse8I GATNNNNATC 2 cut(s) 1696, 1799
BseBI CCWGG 5 cut(s) 110, 243, 287, 326, 1829
BseCI ATCGAT 1 cut(s) 1803
BseDI CCNNGG 8 cut(s) 752, 982, 1040, 1342, 1627, 1753, 1846, 1921
BseGI GGATG 4 cut(s) 827, 942, 1036, 1578
BseJI GATNNNNATC 2 cut(s) 1696, 1799
BseLI CCNNNNNNNGG 4 cut(s) 525, 853, 1205, 1348
BseMI GCAATG 2 cut(s) 753, 1504
BseMII CTCAG 2 cut(s) 287, 1451
BseNI ACTGG 3 cut(s) 204, 702, 1733
BseRI GAGGAG 1 cut(s) 1188
BseSI GKGCMC 1 cut(s) 1753
BseXI GCAGC 9 cut(s) 518, 589, 657, 705, 730, 965, 1057, 1100, 1420
BseYI CCCAGC 1 cut(s) 1746
BshFI GGCC 3 cut(s) 644, 767, 1751
BshNI GGYRCC 1 cut(s) 33
BshVI ATCGAT 1 cut(s) 1803
BsiHKAI GWGCWC 2 cut(s) 68, 1845
BsiHKCI CYCGRG 3 cut(s) 503, 1343, 1403
BsiSI CCGG 3 cut(s) 337, 1304, 1420
BslFI GGGAC 1 cut(s) 1864
BslI CCNNNNNNNGG 4 cut(s) 525, 853, 1205, 1348
BsmAI GTCTC 1 cut(s) 1906
BsmFI GGGAC 1 cut(s) 1864
BsmI GAATGC 2 cut(s) 1185, 1436
BsnI GGCC 3 cut(s) 644, 767, 1751
BsoBI CYCGRG 3 cut(s) 503, 1343, 1403
Bsp120I GGGCCC 1 cut(s) 1749
Bsp1286I GDGCHC 3 cut(s) 68, 1753, 1845
Bsp143I GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
BspACI CCGC 3 cut(s) 363, 940, 1176
BspANI GGCC 3 cut(s) 644, 767, 1751
BspCNI CTCAG 2 cut(s) 288, 1452
BspDI ATCGAT 1 cut(s) 1803
BspHI TCATGA 1 cut(s) 888
BspLI GGNNCC 6 cut(s) 9, 35, 509, 1363, 1751, 1927
BspMAI CTGCAG 1 cut(s) 1552
BspPI GGATC 3 cut(s) 409, 1920, 1933
BspQI GCTCTTC 1 cut(s) 1833
BspT107I GGYRCC 1 cut(s) 33
BspTI CTTAAG 1 cut(s) 965
BsrDI GCAATG 2 cut(s) 753, 1504
BsrI ACTGG 3 cut(s) 204, 702, 1733
BssECI CCNNGG 8 cut(s) 752, 982, 1040, 1342, 1627, 1753, 1846, 1921
BssMI GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
BssT1I CCWWGG 7 cut(s) 752, 982, 1040, 1627, 1753, 1846, 1921
Bst2UI CCWGG 5 cut(s) 110, 243, 287, 326, 1829
Bst4CI ACNGT 4 cut(s) 602, 763, 1163, 1728
Bst6I CTCTTC 3 cut(s) 501, 1723, 1833
BstAFI CTTAAG 1 cut(s) 965
BstAPI GCANNNNNTGC 1 cut(s) 1638
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 3 cut(s) 296, 1460, 1941
BstF5I GGATG 4 cut(s) 827, 942, 1036, 1578
BstHHI GCGC 3 cut(s) 1335, 1705, 1858
BstKTI GATC 9 cut(s) 417, 1059, 1141, 1168, 1564, 1569, 1716, 1812, 1928
BstMAI GTCTC 1 cut(s) 1906
BstMBI GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
BstMWI GCNNNNNNNGC 8 cut(s) 238, 481, 577, 670, 1182, 1638, 1664, 1862
BstNI CCWGG 5 cut(s) 110, 243, 287, 326, 1829
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 6 cut(s) 108, 241, 285, 324, 335, 1827
BstSFI CTRYAG 4 cut(s) 930, 1336, 1548, 1557
BstSLI GKGCMC 1 cut(s) 1753
BstV1I GCAGC 9 cut(s) 518, 589, 657, 705, 730, 965, 1057, 1100, 1420
BstV2I GAAGAC 2 cut(s) 239, 1536
BstX2I RGATCY 4 cut(s) 414, 1561, 1713, 1925
BstYI RGATCY 4 cut(s) 414, 1561, 1713, 1925
Bsu15I ATCGAT 1 cut(s) 1803
BsuI GTATCC 1 cut(s) 1675
BsuRI GGCC 3 cut(s) 644, 767, 1751
BsuTUI ATCGAT 1 cut(s) 1803
BtsCI GGATG 4 cut(s) 827, 942, 1036, 1578
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 7 cut(s) 27, 380, 695, 768, 1159, 1724, 1740
Cac8I GCNNGC 1 cut(s) 234
CciI TCATGA 1 cut(s) 888
CfoI GCGC 3 cut(s) 1335, 1705, 1858
Cfr13I GGNCC 6 cut(s) 8, 97, 508, 766, 1749, 1750
ClaI ATCGAT 1 cut(s) 1803
CseI GACGC 1 cut(s) 1630
CsiI ACCWGGT 1 cut(s) 285
Csp6I GTAC 1 cut(s) 34
CspCI CAANNNNNGTGG 2 cut(s) 684, 719
CviAII CATG 6 cut(s) 233, 640, 660, 853, 889, 1054
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 3 cut(s) 296, 1460, 1941
DpnI GATC 9 cut(s) 416, 1058, 1140, 1167, 1563, 1568, 1715, 1811, 1927
DpnII GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
DrdI GACNNNNNNGTC 1 cut(s) 1483
DseDI GACNNNNNNGTC 1 cut(s) 1483
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 3 cut(s) 501, 1723, 1833
EarI CTCTTC 3 cut(s) 501, 1723, 1833
Ecl136II GAGCTC 1 cut(s) 66
Eco130I CCWWGG 7 cut(s) 752, 982, 1040, 1627, 1753, 1846, 1921
Eco24I GRGCYC 2 cut(s) 68, 1753
Eco47I GGWCC 3 cut(s) 8, 97, 508
Eco53kI GAGCTC 1 cut(s) 66
Eco57I CTGAAG 2 cut(s) 393, 426
Eco88I CYCGRG 3 cut(s) 503, 1343, 1403
EcoICRI GAGCTC 1 cut(s) 66
EcoO109I RGGNCCY 4 cut(s) 8, 97, 508, 1750
EcoRII CCWGG 5 cut(s) 108, 241, 285, 324, 1827
EcoT14I CCWWGG 7 cut(s) 752, 982, 1040, 1627, 1753, 1846, 1921
EcoT38I GRGCYC 2 cut(s) 68, 1753
ErhI CCWWGG 7 cut(s) 752, 982, 1040, 1627, 1753, 1846, 1921
FaeI CATG 6 cut(s) 236, 643, 663, 856, 892, 1057
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 1 cut(s) 1864
FatI CATG 6 cut(s) 232, 639, 659, 852, 888, 1053
FauI CCCGC 1 cut(s) 370
FbaI TGATCA 1 cut(s) 1566
FokI GGATG 4 cut(s) 834, 929, 1023, 1565
FriOI GRGCYC 2 cut(s) 68, 1753
FspBI CTAG 1 cut(s) 1449
FspI TGCGCA 2 cut(s) 1704, 1857
GlaI GCGC 3 cut(s) 1334, 1704, 1857
GsaI CCCAGC 1 cut(s) 1750
HaeIII GGCC 3 cut(s) 644, 767, 1751
HapII CCGG 3 cut(s) 337, 1304, 1420
HgaI GACGC 1 cut(s) 1630
HhaI GCGC 3 cut(s) 1335, 1705, 1858
Hin1II CATG 6 cut(s) 236, 643, 663, 856, 892, 1057
Hin6I GCGC 3 cut(s) 1333, 1703, 1856
HinP1I GCGC 3 cut(s) 1333, 1703, 1856
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 154, 1531
HinfI GANTC 7 cut(s) 162, 687, 727, 1385, 1406, 1423, 1832
HpaII CCGG 3 cut(s) 337, 1304, 1420
HphI GGTGA 3 cut(s) 1070, 1298, 1609
Hpy166II GTNNAC 5 cut(s) 148, 292, 1159, 1476, 1600
Hpy188I TCNGA 5 cut(s) 373, 726, 1566, 1587, 1837
Hpy188III TCNNGA 3 cut(s) 251, 889, 1105
Hpy8I GTNNAC 5 cut(s) 148, 292, 1159, 1476, 1600
Hpy99I CGWCG 1 cut(s) 1419
HpyAV CCTTC 4 cut(s) 136, 378, 401, 1592
HpyCH4III ACNGT 4 cut(s) 602, 763, 1163, 1728
HpyCH4IV ACGT 1 cut(s) 1602
HpyF10VI GCNNNNNNNGC 8 cut(s) 238, 481, 577, 670, 1182, 1638, 1664, 1862
HpyF3I CTNAG 3 cut(s) 296, 1460, 1941
HpySE526I ACGT 1 cut(s) 1602
Hsp92II CATG 6 cut(s) 236, 643, 663, 856, 892, 1057
HspAI GCGC 3 cut(s) 1333, 1703, 1856
KpnI GGTACC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 1566
Kzo9I GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
LguI GCTCTTC 1 cut(s) 1833
LmnI GCTCC 2 cut(s) 528, 1367
Lsp1109I GCAGC 9 cut(s) 518, 589, 657, 705, 730, 965, 1057, 1100, 1420
LweI GCATC 7 cut(s) 322, 826, 871, 951, 1154, 1320, 1896
MabI ACCWGGT 1 cut(s) 285
MaeI CTAG 1 cut(s) 1449
MaeII ACGT 1 cut(s) 1602
MaeIII GTNAC 4 cut(s) 53, 514, 793, 1574
MalI GATC 9 cut(s) 416, 1058, 1140, 1167, 1563, 1568, 1715, 1811, 1927
MboI GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
MboII GAAGA 7 cut(s) 239, 386, 488, 1292, 1536, 1710, 1850
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 4 cut(s) 414, 1561, 1713, 1925
MhlI GDGCHC 3 cut(s) 68, 1753, 1845
MlyI GAGTC 2 cut(s) 1415, 1432
MmeI TCCRAC 1 cut(s) 1659
MseI TTAA 6 cut(s) 182, 275, 629, 774, 966, 1491
MslI CAYNNNNRTG 2 cut(s) 870, 947
MspA1I CMGCKG 2 cut(s) 623, 1070
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 3 cut(s) 337, 1304, 1420
MspR9I CCNGG 6 cut(s) 110, 243, 287, 326, 337, 1829
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 2 cut(s) 1185, 1436
MvaI CCWGG 5 cut(s) 110, 243, 287, 326, 1829
MwoI GCNNNNNNNGC 8 cut(s) 238, 481, 577, 670, 1182, 1638, 1664, 1862
NciI CCSGG 1 cut(s) 337
NdeII GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
NlaIII CATG 6 cut(s) 236, 643, 663, 856, 892, 1057
NlaIV GGNNCC 6 cut(s) 9, 35, 509, 1363, 1751, 1927
NmuCI GTSAC 2 cut(s) 53, 793
NsbI TGCGCA 2 cut(s) 1704, 1857
NspI RCATGY 1 cut(s) 236
PaeI GCATGC 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 1403
PagI TCATGA 1 cut(s) 888
PciSI GCTCTTC 1 cut(s) 1833
PctI GAATGC 2 cut(s) 1185, 1436
PfeI GAWTC 5 cut(s) 162, 687, 727, 1385, 1832
PleI GAGTC 2 cut(s) 1414, 1431
PpsI GAGTC 2 cut(s) 1414, 1431
PpuMI RGGWCCY 3 cut(s) 8, 97, 508
Psp124BI GAGCTC 1 cut(s) 68
Psp5II RGGWCCY 3 cut(s) 8, 97, 508
Psp6I CCWGG 5 cut(s) 108, 241, 285, 324, 1827
PspFI CCCAGC 1 cut(s) 1746
PspGI CCWGG 5 cut(s) 108, 241, 285, 324, 1827
PspN4I GGNNCC 6 cut(s) 9, 35, 509, 1363, 1751, 1927
PspOMI GGGCCC 1 cut(s) 1749
PspPI GGNCC 6 cut(s) 8, 97, 508, 766, 1749, 1750
PspPPI RGGWCCY 3 cut(s) 8, 97, 508
PspXI VCTCGAGB 1 cut(s) 1403
PstI CTGCAG 1 cut(s) 1552
PsuI RGATCY 4 cut(s) 414, 1561, 1713, 1925
PvuII CAGCTG 2 cut(s) 623, 1070
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 2 cut(s) 870, 947
SacI GAGCTC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 1833
SaqAI TTAA 6 cut(s) 182, 275, 629, 774, 966, 1491
Sau3AI GATC 9 cut(s) 414, 1056, 1138, 1165, 1561, 1566, 1713, 1809, 1925
Sau96I GGNCC 6 cut(s) 8, 97, 508, 766, 1749, 1750
SchI GAGTC 2 cut(s) 1415, 1432
ScrFI CCNGG 6 cut(s) 110, 243, 287, 326, 337, 1829
SduI GDGCHC 3 cut(s) 68, 1753, 1845
SexAI ACCWGGT 1 cut(s) 285
SfaNI GCATC 7 cut(s) 322, 826, 871, 951, 1154, 1320, 1896
SfcI CTRYAG 4 cut(s) 930, 1336, 1548, 1557
Sfr274I CTCGAG 1 cut(s) 1403
SinI GGWCC 3 cut(s) 8, 97, 508
SlaI CTCGAG 1 cut(s) 1403
SmiMI CAYNNNNRTG 2 cut(s) 870, 947
SmlI CTYRAG 4 cut(s) 151, 965, 1000, 1403
SmoI CTYRAG 4 cut(s) 151, 965, 1000, 1403
SphI GCATGC 1 cut(s) 236
SsiI CCGC 3 cut(s) 363, 940, 1176
SspI AATATT 4 cut(s) 269, 778, 789, 1015
SspMI CTAG 1 cut(s) 1449
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 6 cut(s) 108, 241, 285, 324, 335, 1827
StyI CCWWGG 7 cut(s) 752, 982, 1040, 1627, 1753, 1846, 1921
TaaI ACNGT 4 cut(s) 602, 763, 1163, 1728
TaiI ACGT 1 cut(s) 1605
TaqI TCGA 6 cut(s) 258, 321, 1121, 1404, 1803, 1881
TaqII GACCGA 1 cut(s) 606
TauI GCSGC 1 cut(s) 942
TfiI GAWTC 5 cut(s) 162, 687, 727, 1385, 1832
Tru1I TTAA 6 cut(s) 182, 275, 629, 774, 966, 1491
Tru9I TTAA 6 cut(s) 182, 275, 629, 774, 966, 1491
TscAI CASTG 7 cut(s) 27, 387, 702, 768, 1166, 1731, 1740
TseFI GTSAC 2 cut(s) 53, 793
TseI GCWGC 9 cut(s) 531, 577, 670, 718, 743, 978, 1070, 1113, 1433
Tsp45I GTSAC 2 cut(s) 53, 793
TspDTI ATGAA 4 cut(s) 443, 1178, 1334, 1809
TspGWI ACGGA 2 cut(s) 407, 860
TspRI CASTG 7 cut(s) 27, 387, 702, 768, 1166, 1731, 1740
Vha464I CTTAAG 1 cut(s) 965
VpaK11BI GGWCC 3 cut(s) 8, 97, 508
XceI RCATGY 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 1403
XspI CTAG 1 cut(s) 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.