Rh5BG237300

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008363
Physical Location & Seq
Forward (+)
28315708 .. 28324068
8361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG237300.1

Sequence Viewer

Length: 2250 bp
ATGAGGGGTCCTTTGGGGGCAGTGATAGGGAGGTACCCATCAAGTGATGGGAGTGACCAGATGAGCTCAATCATCAGGCACAACAGGAAATGCAAGGACCTTGTCTTCCTGGTCATCTTTATAGCCTTCTGGGTTGCTATGATTGTCAACTCAAGCTTTGGATTCAATCAAGGAAACCCATTAAGGCTCACTTATGGACTGGACTACAAAGGAAATGTTTGTGGCGACAGGCATGCAAAACCTGGTCTTCGGGAACTCGAACTCAAATATTGGTTAAATCCAAACCAGGTTTACCTGAGTGGTCTAAAAGACAATCAGTTCGACCTGGCTGATGCCCGGAGTATATGCCTATTGGATTGCCCCGCCCCTTCTGAAGATACACTGAATTGGGTGTGTGATTACCCTGAAGGAGAGATCCGTCTTTCAATGAATGATTGGATTGACAGGAATTATGATTATTTTGAGTTCCTTACTCCTGAAATGCGAAATGCCTCTCTTCAACTTCGGGGTCCTTGTTACCCTGTCATATTTCCTAGTGTAAACGTCTATTGGAGCTGCCAATTTATTGCCCGTCCATCAAACAATTCGTTGAAGCATTGGCAGCAAATGGGTGGAGTGAATATTAATGAGGACATTATTATAGACAAATCTATTCACAGTTCCATCAATTCTCGGTCAGCTGTCTTAAAGAGATACATGGCCGATATTGGAAAGTCATGGCGAGTGCTGCTTGTTTGTGGAGGAATCGTGCCACTGGCTTTGTCATTGATTTGGCTGCTTCTGATTCGTCATTTTGTTACTGCAATGCCTTGGATAACAGTGGCCCTCTTTAATATTCTCATAATATTAGTCACAATGTTTTACTACTTGAAAGTAGATCCTATATTACTTGTCCCCCAAAGGGCAGAGAGACAGCAGGAGGAAGGGGGGGGGGTGTTGTTCCGAGAGGGAAGATTGAGTACAAAAATTGGAATTGTGTTTATTTATGTCCAATATGTTGTCTTCTACAAGGCTGGATGGATAGGAAATGATGCCATCTCCCCAATCATTGGTGAGCACGATCCGTACATTCGCATAATTGGAAGGGAGTTACATCATCTCCGTGCTGTTGCAGTTCTCATGACCTTTATTATGGTTGTTTCTGTTCTTACATCAATTGCTATAGTTCGCCGCATCCTTATGGCAACATCTGTCCTTAAGGTTGCTGCCAAGGTCATAGGAGAAGCTCAAGCACTCATAATATTTCCAATAATTCCATATACCATCCTTGGAGTTTTTTACATGATCTGGTTTTCAGCTGCTTTTCACCTGTTCAGTTCTGGTCAAGTAGTCCAGAATAGCTGCAACTCGAACTGCTGTGCCTATGATCTTGCATCAAAACAAGTTCACTGTGATCGTTGTTGCGGTTATAGCATTCATTACACTCCTCATATTGGAATTGCTATCCTTTTCCACCTATTTGGGTGTTATTGGGCTACACAGTTTTTCAAAGCAGCCTCTTCAACTGTTATTGCTGGTTCTGTGGCCTCTTACTATTGGGCTCGTGGTGAAACATCAGGTGAAATACCATTTCTTCCGGTCTTTTCCTCTATGAAACGGCTGATGCGCTATAGCCTCGGGTCGGTGGCTCTTGGCTCCCTGATTGTGTCATTTGTGGAATCAATAAGGTTTATGCTCGAGTCAATTCGTCGCCGATTAAAAGTTTCTGGTACCACGCCTAATAACTGGTTGGGAAAGGCTGCATTCTCTACTGCTAGGTTTTGCCTGAGCTGTATTGAGTGGACAATCAAGTCGGTTAATCGCAATGCCTACATTATGATTGCTATAACAGGCAAAAGCTTCTGCAAGTCTTCTGCAGTTGCTACAGATCTGATCATCAGTAACATCCTTCGGATAGGGAGGGTGAACGTGATTGGAGATGTGATACTATTCCTTGGCAAATTGTGTGTCAGCCTATCAACTGCCCTTTTTGCTTTTCTTATGTTGGATACCCACAGATACAAATCTGCGCATAACAAGATCTCTTCTCCACTGTTTCCAGTGTTGGTTTGCTGGGCCCTTGGTTATGTGGTTGCTACACTTTTCTTTGCTGTGGTGGAGATGTCAATCGATACGATCATTCTTTCATTCTGCCAGGATTCCGAAGAGCACCAAGGGACTGCGCAGTATGCCCCTCCACTTCTCATCGAAACTCTAAATGACGAAAATGAGATGCAGAGACTTACCCAAGGATCCAATGATATACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000139 GO:0000325 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0005215 GO:0005326 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006836 GO:0007275 GO:0007389 GO:0008104 GO:0008150 GO:0008324 GO:0008519 GO:0009505 GO:0009506 GO:0009524 GO:0009551 GO:0009663 GO:0009705 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010008 GO:0010051 GO:0010065 GO:0010067 GO:0010087 GO:0010088 GO:0010154 GO:0010496 GO:0010497 GO:0010817 GO:0012505 GO:0015031 GO:0015075 GO:0015101 GO:0015220 GO:0015695 GO:0015696 GO:0015833 GO:0015871 GO:0016020 GO:0016043 GO:0016192 GO:0022414 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0030054 GO:0030100 GO:0030154 GO:0030312 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0033036 GO:0034220 GO:0034330 GO:0040008 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044426 GO:0044431 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044462 GO:0044464 GO:0045184 GO:0045216 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051510 GO:0055044 GO:0055085 GO:0055088 GO:0060627 GO:0061458 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072488 GO:0090603 GO:0097218 GO:0097708 GO:0098588 GO:0098655 GO:0098791 GO:0098805 GO:0099402 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko05231 map05231
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

749

Amino Acids

84.13

Weight (kDa)

8.72

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 373 - 715 3.1e-89 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1789
Acc16I TGCGCA 2 cut(s) 2010, 2163
Acc65I GGTACC 2 cut(s) 33, 1709
AccB1I GGYRCC 2 cut(s) 33, 1709
AccB7I CCANNNNNTGG 1 cut(s) 1049
AciI CCGC 3 cut(s) 363, 1171, 1404
AclWI GGATC 5 cut(s) 409, 872, 1055, 2226, 2239
AcoI YGGCCR 1 cut(s) 699
AcuI CTGAAG 2 cut(s) 393, 426
AfaI GTAC 4 cut(s) 35, 961, 1067, 1711
AfiI CCNNNNNNNGG 4 cut(s) 901, 1049, 1433, 1621
AflII CTTAAG 1 cut(s) 1196
AgsI TTSAA 7 cut(s) 166, 426, 500, 592, 871, 1489, 1503
AjnI CCWGG 5 cut(s) 108, 241, 285, 324, 2133
AluBI AGCT 9 cut(s) 66, 156, 555, 680, 1226, 1298, 1341, 1770, 1839
AluI AGCT 9 cut(s) 66, 156, 555, 680, 1226, 1298, 1341, 1770, 1839
Alw21I GWGCWC 3 cut(s) 68, 1059, 2151
Alw26I GTCTC 2 cut(s) 904, 2212
AlwI GGATC 5 cut(s) 409, 872, 1055, 2226, 2239
Ama87I CYCGRG 2 cut(s) 1616, 1676
AoxI GGCC 4 cut(s) 699, 822, 1524, 2055
ApaI GGGCCC 1 cut(s) 2059
ApeKI GCWGC 9 cut(s) 555, 601, 727, 775, 1205, 1298, 1341, 1493, 1739
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 1932, 1964
AseI ATTAAT 1 cut(s) 624
Asp718I GGTACC 2 cut(s) 33, 1709
AspLEI GCGC 3 cut(s) 1608, 2011, 2164
AspS9I GGNCC 6 cut(s) 8, 97, 509, 823, 2055, 2056
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 5 cut(s) 1064, 1298, 1559, 1571, 1915
AvaI CYCGRG 2 cut(s) 1616, 1676
AvaII GGWCC 3 cut(s) 8, 97, 509
BaeGI GKGCMC 1 cut(s) 2059
BamHI GGATCC 1 cut(s) 2231
BanI GGYRCC 2 cut(s) 33, 1709
BanII GRGCYC 3 cut(s) 68, 1543, 2059
BarI GAAGNNNNNNTAC 2 cut(s) 943, 975
BauI CACGAG 1 cut(s) 1542
BbsI GAAGAC 4 cut(s) 97, 239, 994, 1842
Bbv12I GWGCWC 3 cut(s) 68, 1059, 2151
BbvI GCAGC 9 cut(s) 542, 613, 714, 762, 1192, 1285, 1328, 1505, 1726
BccI CCATC 7 cut(s) 41, 46, 583, 671, 1011, 1043, 1271
BceAI ACGGC 1 cut(s) 1613
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 5 cut(s) 110, 243, 287, 326, 2135
BciVI GTATCC 1 cut(s) 1981
BclI TGATCA 1 cut(s) 1872
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 2 cut(s) 904, 2212
BfaI CTAG 2 cut(s) 534, 1755
BfmI CTRYAG 4 cut(s) 1161, 1609, 1854, 1863
BfrI CTTAAG 1 cut(s) 1196
BfuI GTATCC 1 cut(s) 1981
BglII AGATCT 2 cut(s) 1867, 2019
Bme1390I CCNGG 6 cut(s) 110, 243, 287, 326, 337, 2135
Bme18I GGWCC 3 cut(s) 8, 97, 509
BmeT110I CYCGRG 2 cut(s) 1616, 1676
BmgT120I GGNCC 6 cut(s) 8, 97, 509, 823, 2055, 2056
BmiI GGNNCC 7 cut(s) 9, 35, 510, 1636, 1711, 2057, 2233
BmrFI CCNGG 6 cut(s) 110, 243, 287, 326, 337, 2135
BmsI GCATC 6 cut(s) 322, 1021, 1182, 1382, 1593, 2202
BpiI GAAGAC 4 cut(s) 97, 239, 994, 1842
Bpu10I CCTNAGC 1 cut(s) 1766
BpuEI CTTGAG 2 cut(s) 136, 1212
BpuMI CCSGG 1 cut(s) 337
Bsa29I ATCGAT 1 cut(s) 2109
BsaBI GATNNNNATC 2 cut(s) 2002, 2105
BsaJI CCNNGG 8 cut(s) 809, 1209, 1267, 1615, 1933, 2059, 2152, 2227
BsaWI WCCGGW 1 cut(s) 1576
BsaXI ACNNNNNCTCC 4 cut(s) 732, 762, 1083, 1113
Bsc4I CCNNNNNNNGG 4 cut(s) 901, 1049, 1433, 1621
Bse1I ACTGG 4 cut(s) 204, 759, 1730, 2039
Bse3DI GCAATG 2 cut(s) 810, 1810
Bse8I GATNNNNATC 2 cut(s) 2002, 2105
BseBI CCWGG 5 cut(s) 110, 243, 287, 326, 2135
BseCI ATCGAT 1 cut(s) 2109
BseDI CCNNGG 8 cut(s) 809, 1209, 1267, 1615, 1933, 2059, 2152, 2227
BseGI GGATG 4 cut(s) 1022, 1173, 1263, 1884
BseJI GATNNNNATC 2 cut(s) 2002, 2105
BseLI CCNNNNNNNGG 4 cut(s) 901, 1049, 1433, 1621
BseMI GCAATG 2 cut(s) 810, 1810
BseMII CTCAG 2 cut(s) 287, 1757
BseNI ACTGG 4 cut(s) 204, 759, 1730, 2039
BseRI GAGGAG 1 cut(s) 1416
BseSI GKGCMC 1 cut(s) 2059
BseXI GCAGC 9 cut(s) 542, 613, 714, 762, 1192, 1285, 1328, 1505, 1726
BseYI CCCAGC 1 cut(s) 2052
BshFI GGCC 4 cut(s) 701, 824, 1526, 2057
BshNI GGYRCC 2 cut(s) 33, 1709
BshVI ATCGAT 1 cut(s) 2109
BsiHKAI GWGCWC 3 cut(s) 68, 1059, 2151
BsiHKCI CYCGRG 2 cut(s) 1616, 1676
BsiSI CCGG 2 cut(s) 337, 1577
BslFI GGGAC 2 cut(s) 878, 2170
BslI CCNNNNNNNGG 4 cut(s) 901, 1049, 1433, 1621
BsmAI GTCTC 2 cut(s) 904, 2212
BsmFI GGGAC 2 cut(s) 878, 2170
BsmI GAATGC 2 cut(s) 1413, 1742
BsnI GGCC 4 cut(s) 701, 824, 1526, 2057
BsoBI CYCGRG 2 cut(s) 1616, 1676
Bsp120I GGGCCC 1 cut(s) 2055
Bsp1286I GDGCHC 5 cut(s) 68, 1059, 1543, 2059, 2151
BspACI CCGC 3 cut(s) 363, 1171, 1404
BspANI GGCC 4 cut(s) 701, 824, 1526, 2057
BspCNI CTCAG 2 cut(s) 288, 1758
BspDI ATCGAT 1 cut(s) 2109
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 7 cut(s) 9, 35, 510, 1636, 1711, 2057, 2233
BspMAI CTGCAG 1 cut(s) 1858
BspPI GGATC 5 cut(s) 409, 872, 1055, 2226, 2239
BspQI GCTCTTC 1 cut(s) 2139
BspT107I GGYRCC 2 cut(s) 33, 1709
BspTI CTTAAG 1 cut(s) 1196
BsrDI GCAATG 2 cut(s) 810, 1810
BsrI ACTGG 4 cut(s) 204, 759, 1730, 2039
BssECI CCNNGG 8 cut(s) 809, 1209, 1267, 1615, 1933, 2059, 2152, 2227
BssSI CACGAG 1 cut(s) 1542
BssT1I CCWWGG 7 cut(s) 809, 1209, 1267, 1933, 2059, 2152, 2227
Bst2BI CACGAG 1 cut(s) 1542
Bst2UI CCWGG 5 cut(s) 110, 243, 287, 326, 2135
Bst4CI ACNGT 6 cut(s) 659, 820, 1391, 1482, 1507, 2034
Bst6I CTCTTC 4 cut(s) 501, 1504, 2029, 2139
BstAFI CTTAAG 1 cut(s) 1196
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 3 cut(s) 296, 1766, 2247
BstF5I GGATG 4 cut(s) 1022, 1173, 1263, 1884
BstHHI GCGC 3 cut(s) 1608, 2011, 2164
BstMAI GTCTC 2 cut(s) 904, 2212
BstMWI GCNNNNNNNGC 5 cut(s) 601, 727, 1410, 1970, 2168
BstNI CCWGG 5 cut(s) 110, 243, 287, 326, 2135
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 6 cut(s) 108, 241, 285, 324, 335, 2133
BstSFI CTRYAG 4 cut(s) 1161, 1609, 1854, 1863
BstSLI GKGCMC 1 cut(s) 2059
BstV1I GCAGC 9 cut(s) 542, 613, 714, 762, 1192, 1285, 1328, 1505, 1726
BstV2I GAAGAC 4 cut(s) 97, 239, 994, 1842
BstX2I RGATCY 5 cut(s) 414, 877, 1867, 2019, 2231
BstXI CCANNNNNNTGG 1 cut(s) 1460
BstYI RGATCY 5 cut(s) 414, 877, 1867, 2019, 2231
Bsu15I ATCGAT 1 cut(s) 2109
BsuI GTATCC 1 cut(s) 1981
BsuRI GGCC 4 cut(s) 701, 824, 1526, 2057
BsuTUI ATCGAT 1 cut(s) 2109
BtsCI GGATG 4 cut(s) 1022, 1173, 1263, 1884
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 7 cut(s) 27, 380, 752, 825, 1387, 2030, 2046
Cac8I GCNNGC 1 cut(s) 234
CciI TCATGA 1 cut(s) 1119
CfoI GCGC 3 cut(s) 1608, 2011, 2164
Cfr13I GGNCC 6 cut(s) 8, 97, 509, 823, 2055, 2056
ClaI ATCGAT 1 cut(s) 2109
CsiI ACCWGGT 2 cut(s) 241, 285
Csp6I GTAC 4 cut(s) 34, 960, 1066, 1710
CspCI CAANNNNNGTGG 2 cut(s) 741, 776
CviAII CATG 5 cut(s) 233, 697, 717, 1120, 1282
CviQI GTAC 4 cut(s) 34, 960, 1066, 1710
DdeI CTNAG 3 cut(s) 296, 1766, 2247
DrdI GACNNNNNNGTC 1 cut(s) 1789
DseDI GACNNNNNNGTC 1 cut(s) 1789
EaeI YGGCCR 1 cut(s) 699
Eam1104I CTCTTC 4 cut(s) 501, 1504, 2029, 2139
EarI CTCTTC 4 cut(s) 501, 1504, 2029, 2139
Ecl136II GAGCTC 1 cut(s) 66
Eco130I CCWWGG 7 cut(s) 809, 1209, 1267, 1933, 2059, 2152, 2227
Eco24I GRGCYC 3 cut(s) 68, 1543, 2059
Eco47I GGWCC 3 cut(s) 8, 97, 509
Eco53kI GAGCTC 1 cut(s) 66
Eco57I CTGAAG 2 cut(s) 393, 426
Eco88I CYCGRG 2 cut(s) 1616, 1676
EcoICRI GAGCTC 1 cut(s) 66
EcoO109I RGGNCCY 4 cut(s) 8, 97, 509, 2056
EcoRII CCWGG 5 cut(s) 108, 241, 285, 324, 2133
EcoT14I CCWWGG 7 cut(s) 809, 1209, 1267, 1933, 2059, 2152, 2227
EcoT38I GRGCYC 3 cut(s) 68, 1543, 2059
ErhI CCWWGG 7 cut(s) 809, 1209, 1267, 1933, 2059, 2152, 2227
FaeI CATG 5 cut(s) 236, 700, 720, 1123, 1285
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 2 cut(s) 878, 2170
FatI CATG 5 cut(s) 232, 696, 716, 1119, 1281
FauI CCCGC 1 cut(s) 370
FbaI TGATCA 1 cut(s) 1872
FokI GGATG 4 cut(s) 1029, 1160, 1250, 1871
FriOI GRGCYC 3 cut(s) 68, 1543, 2059
FspBI CTAG 2 cut(s) 534, 1755
FspI TGCGCA 2 cut(s) 2010, 2163
GlaI GCGC 3 cut(s) 1607, 2010, 2163
GsaI CCCAGC 1 cut(s) 2056
HaeIII GGCC 4 cut(s) 701, 824, 1526, 2057
HapII CCGG 2 cut(s) 337, 1577
HhaI GCGC 3 cut(s) 1608, 2011, 2164
Hin1II CATG 5 cut(s) 236, 700, 720, 1123, 1285
Hin6I GCGC 3 cut(s) 1606, 2009, 2162
HinP1I GCGC 3 cut(s) 1606, 2009, 2162
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 154, 1837
HinfI GANTC 6 cut(s) 162, 744, 784, 1658, 1679, 2138
HpaII CCGG 2 cut(s) 337, 1577
HphI GGTGA 5 cut(s) 1064, 1298, 1559, 1571, 1915
Hpy166II GTNNAC 6 cut(s) 148, 292, 541, 1387, 1782, 1906
Hpy188I TCNGA 6 cut(s) 373, 783, 944, 1872, 1893, 2143
Hpy188III TCNNGA 4 cut(s) 251, 476, 1120, 1333
Hpy8I GTNNAC 6 cut(s) 148, 292, 541, 1387, 1782, 1906
Hpy99I CGWCG 1 cut(s) 1692
HpyAV CCTTC 6 cut(s) 136, 378, 401, 917, 1077, 1898
HpyCH4III ACNGT 6 cut(s) 659, 820, 1391, 1482, 1507, 2034
HpyCH4IV ACGT 2 cut(s) 543, 1908
HpyF10VI GCNNNNNNNGC 5 cut(s) 601, 727, 1410, 1970, 2168
HpyF3I CTNAG 3 cut(s) 296, 1766, 2247
HpySE526I ACGT 2 cut(s) 543, 1908
Hsp92II CATG 5 cut(s) 236, 700, 720, 1123, 1285
HspAI GCGC 3 cut(s) 1606, 2009, 2162
KpnI GGTACC 2 cut(s) 37, 1713
Ksp22I TGATCA 1 cut(s) 1872
LguI GCTCTTC 1 cut(s) 2139
LmnI GCTCC 2 cut(s) 552, 1640
Lsp1109I GCAGC 9 cut(s) 542, 613, 714, 762, 1192, 1285, 1328, 1505, 1726
LweI GCATC 6 cut(s) 322, 1021, 1182, 1382, 1593, 2202
MabI ACCWGGT 2 cut(s) 241, 285
MaeI CTAG 2 cut(s) 534, 1755
MaeII ACGT 2 cut(s) 543, 1908
MaeIII GTNAC 6 cut(s) 53, 515, 796, 850, 1089, 1880
MfeI CAATTG 1 cut(s) 1155
MflI RGATCY 5 cut(s) 414, 877, 1867, 2019, 2231
MhlI GDGCHC 5 cut(s) 68, 1059, 1543, 2059, 2151
MlyI GAGTC 1 cut(s) 1688
MmeI TCCRAC 1 cut(s) 1965
MseI TTAA 8 cut(s) 182, 275, 624, 686, 831, 1197, 1697, 1797
MslI CAYNNNNRTG 2 cut(s) 1101, 1178
MspA1I CMGCKG 2 cut(s) 680, 1298
MspCI CTTAAG 1 cut(s) 1196
MspI CCGG 2 cut(s) 337, 1577
MspR9I CCNGG 6 cut(s) 110, 243, 287, 326, 337, 2135
MunI CAATTG 1 cut(s) 1155
Mva1269I GAATGC 2 cut(s) 1413, 1742
MvaI CCWGG 5 cut(s) 110, 243, 287, 326, 2135
MwoI GCNNNNNNNGC 5 cut(s) 601, 727, 1410, 1970, 2168
NciI CCSGG 1 cut(s) 337
NlaIII CATG 5 cut(s) 236, 700, 720, 1123, 1285
NlaIV GGNNCC 7 cut(s) 9, 35, 510, 1636, 1711, 2057, 2233
NmuCI GTSAC 2 cut(s) 53, 850
NsbI TGCGCA 2 cut(s) 2010, 2163
NspI RCATGY 1 cut(s) 236
PaeI GCATGC 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 1676
PagI TCATGA 1 cut(s) 1119
PciSI GCTCTTC 1 cut(s) 2139
PctI GAATGC 2 cut(s) 1413, 1742
PfeI GAWTC 5 cut(s) 162, 744, 784, 1658, 2138
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 1049
PleI GAGTC 1 cut(s) 1687
PpsI GAGTC 1 cut(s) 1687
PpuMI RGGWCCY 3 cut(s) 8, 97, 509
PshBI ATTAAT 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 68
Psp5II RGGWCCY 3 cut(s) 8, 97, 509
Psp6I CCWGG 5 cut(s) 108, 241, 285, 324, 2133
PspFI CCCAGC 1 cut(s) 2052
PspGI CCWGG 5 cut(s) 108, 241, 285, 324, 2133
PspN4I GGNNCC 7 cut(s) 9, 35, 510, 1636, 1711, 2057, 2233
PspOMI GGGCCC 1 cut(s) 2055
PspPI GGNCC 6 cut(s) 8, 97, 509, 823, 2055, 2056
PspPPI RGGWCCY 3 cut(s) 8, 97, 509
PspXI VCTCGAGB 1 cut(s) 1676
PstI CTGCAG 1 cut(s) 1858
PsuI RGATCY 5 cut(s) 414, 877, 1867, 2019, 2231
PsyI GACNNNGTC 1 cut(s) 101
PvuII CAGCTG 2 cut(s) 680, 1298
RsaI GTAC 4 cut(s) 35, 961, 1067, 1711
RsaNI GTAC 4 cut(s) 34, 960, 1066, 1710
RseI CAYNNNNRTG 2 cut(s) 1101, 1178
SacI GAGCTC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 2139
SaqAI TTAA 8 cut(s) 182, 275, 624, 686, 831, 1197, 1697, 1797
Sau96I GGNCC 6 cut(s) 8, 97, 509, 823, 2055, 2056
SchI GAGTC 1 cut(s) 1688
ScrFI CCNGG 6 cut(s) 110, 243, 287, 326, 337, 2135
SduI GDGCHC 5 cut(s) 68, 1059, 1543, 2059, 2151
SexAI ACCWGGT 2 cut(s) 241, 285
SfaNI GCATC 6 cut(s) 322, 1021, 1182, 1382, 1593, 2202
SfcI CTRYAG 4 cut(s) 1161, 1609, 1854, 1863
Sfr274I CTCGAG 1 cut(s) 1676
SinI GGWCC 3 cut(s) 8, 97, 509
SlaI CTCGAG 1 cut(s) 1676
SmiMI CAYNNNNRTG 2 cut(s) 1101, 1178
SmlI CTYRAG 4 cut(s) 151, 1196, 1227, 1676
SmoI CTYRAG 4 cut(s) 151, 1196, 1227, 1676
SphI GCATGC 1 cut(s) 236
SsiI CCGC 3 cut(s) 363, 1171, 1404
SspI AATATT 5 cut(s) 269, 622, 835, 846, 1242
SspMI CTAG 2 cut(s) 534, 1755
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 6 cut(s) 108, 241, 285, 324, 335, 2133
StyI CCWWGG 7 cut(s) 809, 1209, 1267, 1933, 2059, 2152, 2227
TaaI ACNGT 6 cut(s) 659, 820, 1391, 1482, 1507, 2034
TaiI ACGT 2 cut(s) 546, 1911
TaqI TCGA 6 cut(s) 258, 321, 1349, 1677, 2109, 2187
TaqII GACCGA 1 cut(s) 663
TatI WGTACW 1 cut(s) 959
TauI GCSGC 1 cut(s) 1173
TfiI GAWTC 5 cut(s) 162, 744, 784, 1658, 2138
Tru1I TTAA 8 cut(s) 182, 275, 624, 686, 831, 1197, 1697, 1797
Tru9I TTAA 8 cut(s) 182, 275, 624, 686, 831, 1197, 1697, 1797
TscAI CASTG 7 cut(s) 27, 387, 759, 825, 1394, 2037, 2046
TseFI GTSAC 2 cut(s) 53, 850
TseI GCWGC 9 cut(s) 555, 601, 727, 775, 1205, 1298, 1341, 1493, 1739
Tsp45I GTSAC 2 cut(s) 53, 850
TspDTI ATGAA 4 cut(s) 443, 1406, 1607, 2115
TspGWI ACGGA 3 cut(s) 407, 1053, 1091
TspRI CASTG 7 cut(s) 27, 387, 759, 825, 1394, 2037, 2046
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 1049
Vha464I CTTAAG 1 cut(s) 1196
VpaK11BI GGWCC 3 cut(s) 8, 97, 509
VspI ATTAAT 1 cut(s) 624
XceI RCATGY 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 1676
XspI CTAG 2 cut(s) 534, 1755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.