RchiOBHm_Chr5g0034311

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008363
Physical Location & Seq
Forward (+)
28155392 .. 28161907
6516 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31333

Sequence Viewer

Length: 2112 bp
ATGAGGGGTCCTTTGGGGGCAGTGATAGGGAGGTACCCATCAAGTGATGGGAGTGACCAGATGAGCTCAATCATCAGGCACAACAGGAAATGCAAGGACCTTGTGTTCCTGGTCATCTTTATAGCCTTCTGGGTTGCTATGATTGTCAACTCAAGCTTTGGATTCAATCAAGGAAACCCATTAAGGCTCACTTATGGACTGGACTACAAAGGAAATGTTTGTGGCGACAGGCATGCAAAACCTGGTCTTCGGGAACTCGAACTCAAATATTGGTTAAATCCAAACCAGGTTTACCTGAGTGGTCTAAAAGACAATCAGTTCGAGCTGGCCGATGCCCGGAGTATATGCCTATTGGATTGCCCCGCCCCTTCTGAAGATACACTGAATTGGGTGTGTGATTACCCTGAAGGAGAGATCCGTCTCGCAATGAATGATTGGATTGACAGGAATTATGATTACTTTGAGTTCCTTACTCCTGAAATGCGAAATGCCTCTCTTCAACTTCGGGGTCCTTGTTACCCTGTCATATTTCCTAGTGTAAACGTCTATTGGAGCTGCCAATTTATTGCTCGTCCGTCAAACAATTCGCTGAAGCATTGGCAGCAAATGGGTGGAGTGAATATTAATGAGGACATTATTATAGACAAATCTATTCACAGTTCCATCAATTCTCGGTCAGCTGTCTTAAAGAGATACATGGCCGATATTGGAAAGTCATGGCGAGTGCTGCTTGTTTGTGGAGGAATCGTGCCACTGGCTTTGTCATTGATTTGGCTGCTTCTGATTCGTCATTTTGTTGCTGCAATGCCTTGGATAACAGTGGCCCTCTTTAATATTCTCATAATATCAGTCACAATGTTTTACTACTTGAAAGCTGGATGGATAGGAAATGATGCCATCTCCCCAATCATTGGTGAGCACGATCCGTACATTCGCATAATTGGAAGGGAGTTACATCATCTCCGTGCTGTTGCAGTTCTCATGACCTTTATTATGGTTGTTTCTGTTCTTACATCAATTGCTATAGTTCGCCGCATCCTTATGGCAACATCTGTCCTTAAGGTTGCTGCCAAGGTCATAGGAGAAGCTCAAGCACTCATAATATTTCCAATAATTCCATATACCATCCTTGGAGTTTTTTACATGATCTGGTTTTCAGCTGCTTTTCACCTGTTCAGTTCTGGTCAAGTAGTCCAGAATAGCTGCAACTCGAACTGCTGTGCCTATGATCTTGCATCAAAACAAGTTCACTGTGATCGTTGTTGCGGTTATAGCATTCATTACACTCCTCATATTGGAATTGCTATCCTTTTCCACCTATTTGGGTGTTATTGGGCTACACAGTTTTTCAAAGCAGCCTCTTCAACTGTTATTGCTGGTTCTGTGGCCTCTTACTATTGGGCTCGTGGTGAAACATCAGGTGAAATACCATTTCTTCCGGTCTTTTCCTCTATGAAACGGCTGATGCGCTATAGCCTCGGGTCGGTGGCTCTTGGCTCCCTGATTGTGTCATTTGTGGAATCAATAAGGTTTATGCTCGAGTCAATTCGTCGCCGATTAAAAGTTTCTGGTACCACGCCTAATAACTGGTTCGGAAAGGCTGCATTCTCTACTGCTAGGTTTTGCCTGAGCTGTATTGAGTGGACAATCAAGTCGGTTAATCGCAATGCCTACATTATGATTGCTATAACAGGCAAAAGCTTCTGCAAGTCTTCTGCAGTTGCTACAGAACTGATCATCAGTAACATCCTTCGGATAGGGAGGGTGAACGTGATTGGAGATGTGATACTATTCCTTGGCAAATTGTGTGTCAGCCTATCAACTGCCCTTTTTGCTTTTCTTATGTTGGATACCCACAGATACAAATCTGCGCATAACAAGATCTCTTCTCCACTGTTTCCAGTGTTGGTTTGCTGGGCCCTTGGTTATGTGGTTGCTACACTTTTCTTTGCTGTGGTGGAGATGTCAATCGATACGATCATTCTTTCATTCTGCCAGGATTCCGAAGAGCACCAAGGGACTGCACAGTATGCCCCTCCACTTCTCATCGAAACTCTAAATGACGAAAATGAGATGCAGAGACTTACCCAAGGATCCAATGATATACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000139 GO:0000325 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0005215 GO:0005326 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006836 GO:0007275 GO:0007389 GO:0008104 GO:0008150 GO:0008324 GO:0008519 GO:0009505 GO:0009506 GO:0009524 GO:0009551 GO:0009663 GO:0009705 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010008 GO:0010051 GO:0010065 GO:0010067 GO:0010087 GO:0010088 GO:0010154 GO:0010496 GO:0010497 GO:0010817 GO:0012505 GO:0015031 GO:0015075 GO:0015101 GO:0015220 GO:0015695 GO:0015696 GO:0015833 GO:0015871 GO:0016020 GO:0016043 GO:0016192 GO:0022414 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0030054 GO:0030100 GO:0030154 GO:0030312 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0033036 GO:0034220 GO:0034330 GO:0040008 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044426 GO:0044431 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044462 GO:0044464 GO:0045184 GO:0045216 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051510 GO:0055044 GO:0055085 GO:0055088 GO:0060627 GO:0061458 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072488 GO:0090603 GO:0097218 GO:0097708 GO:0098588 GO:0098655 GO:0098791 GO:0098805 GO:0099402 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko05231 map05231
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

703

Amino Acids

78.82

Weight (kDa)

8.67

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 327 - 669 2.6e-89 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1651
Acc16I TGCGCA 1 cut(s) 1872
Acc65I GGTACC 2 cut(s) 33, 1571
AccB1I GGYRCC 2 cut(s) 33, 1571
AccB7I CCANNNNNTGG 1 cut(s) 911
AciI CCGC 3 cut(s) 363, 1033, 1266
AclWI GGATC 4 cut(s) 409, 917, 2088, 2101
AcoI YGGCCR 2 cut(s) 327, 699
AcuI CTGAAG 3 cut(s) 393, 426, 611
AfaI GTAC 3 cut(s) 35, 929, 1573
AfiI CCNNNNNNNGG 4 cut(s) 336, 911, 1295, 1483
AflII CTTAAG 1 cut(s) 1058
AgsI TTSAA 5 cut(s) 166, 500, 871, 1351, 1365
AjnI CCWGG 4 cut(s) 108, 241, 285, 1995
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
Alw21I GWGCWC 3 cut(s) 68, 921, 2013
Alw26I GTCTC 2 cut(s) 425, 2074
AlwI GGATC 4 cut(s) 409, 917, 2088, 2101
Ama87I CYCGRG 2 cut(s) 1478, 1538
AoxI GGCC 5 cut(s) 327, 699, 822, 1386, 1917
ApaI GGGCCC 1 cut(s) 1921
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 1794, 1826
AseI ATTAAT 1 cut(s) 624
Asp718I GGTACC 2 cut(s) 33, 1571
AspLEI GCGC 2 cut(s) 1470, 1873
AspS9I GGNCC 6 cut(s) 8, 97, 509, 823, 1917, 1918
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 5 cut(s) 926, 1160, 1421, 1433, 1777
AvaI CYCGRG 2 cut(s) 1478, 1538
AvaII GGWCC 3 cut(s) 8, 97, 509
BaeGI GKGCMC 1 cut(s) 1921
BamHI GGATCC 1 cut(s) 2093
BanI GGYRCC 2 cut(s) 33, 1571
BanII GRGCYC 3 cut(s) 68, 1405, 1921
BauI CACGAG 1 cut(s) 1404
BbsI GAAGAC 2 cut(s) 239, 1704
Bbv12I GWGCWC 3 cut(s) 68, 921, 2013
BccI CCATC 6 cut(s) 41, 46, 671, 873, 905, 1133
BceAI ACGGC 1 cut(s) 1475
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 4 cut(s) 110, 243, 287, 1997
BciVI GTATCC 1 cut(s) 1843
BclI TGATCA 1 cut(s) 1734
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 2 cut(s) 425, 2074
BfaI CTAG 2 cut(s) 534, 1617
BfmI CTRYAG 4 cut(s) 1023, 1471, 1716, 1725
BfrI CTTAAG 1 cut(s) 1058
BfuI GTATCC 1 cut(s) 1843
BglII AGATCT 1 cut(s) 1881
Bme1390I CCNGG 5 cut(s) 110, 243, 287, 337, 1997
Bme18I GGWCC 3 cut(s) 8, 97, 509
BmeT110I CYCGRG 2 cut(s) 1478, 1538
BmgT120I GGNCC 6 cut(s) 8, 97, 509, 823, 1917, 1918
BmiI GGNNCC 7 cut(s) 9, 35, 510, 1498, 1573, 1919, 2095
BmrFI CCNGG 5 cut(s) 110, 243, 287, 337, 1997
BmsI GCATC 6 cut(s) 322, 883, 1044, 1244, 1455, 2064
BpiI GAAGAC 2 cut(s) 239, 1704
Bpu10I CCTNAGC 1 cut(s) 1628
BpuEI CTTGAG 2 cut(s) 136, 1074
BpuMI CCSGG 1 cut(s) 337
Bsa29I ATCGAT 1 cut(s) 1971
BsaBI GATNNNNATC 2 cut(s) 1864, 1967
BsaJI CCNNGG 8 cut(s) 809, 1071, 1129, 1477, 1795, 1921, 2014, 2089
BsaWI WCCGGW 1 cut(s) 1438
BsaXI ACNNNNNCTCC 4 cut(s) 732, 762, 945, 975
Bsc4I CCNNNNNNNGG 4 cut(s) 336, 911, 1295, 1483
Bse1I ACTGG 4 cut(s) 204, 759, 1592, 1901
Bse3DI GCAATG 3 cut(s) 432, 810, 1672
Bse8I GATNNNNATC 2 cut(s) 1864, 1967
BseBI CCWGG 4 cut(s) 110, 243, 287, 1997
BseCI ATCGAT 1 cut(s) 1971
BseDI CCNNGG 8 cut(s) 809, 1071, 1129, 1477, 1795, 1921, 2014, 2089
BseGI GGATG 4 cut(s) 884, 1035, 1125, 1746
BseJI GATNNNNATC 2 cut(s) 1864, 1967
BseLI CCNNNNNNNGG 4 cut(s) 336, 911, 1295, 1483
BseMI GCAATG 3 cut(s) 432, 810, 1672
BseMII CTCAG 2 cut(s) 287, 1619
BseNI ACTGG 4 cut(s) 204, 759, 1592, 1901
BseRI GAGGAG 1 cut(s) 1278
BseSI GKGCMC 1 cut(s) 1921
BseYI CCCAGC 1 cut(s) 1914
BsgI GTGCAG 1 cut(s) 2007
BshFI GGCC 5 cut(s) 329, 701, 824, 1388, 1919
BshNI GGYRCC 2 cut(s) 33, 1571
BshVI ATCGAT 1 cut(s) 1971
BsiHKAI GWGCWC 3 cut(s) 68, 921, 2013
BsiHKCI CYCGRG 2 cut(s) 1478, 1538
BsiSI CCGG 2 cut(s) 337, 1439
BslFI GGGAC 1 cut(s) 2032
BslI CCNNNNNNNGG 4 cut(s) 336, 911, 1295, 1483
BsmAI GTCTC 2 cut(s) 425, 2074
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 1 cut(s) 2032
BsmI GAATGC 2 cut(s) 1275, 1604
BsnI GGCC 5 cut(s) 329, 701, 824, 1388, 1919
BsoBI CYCGRG 2 cut(s) 1478, 1538
Bsp120I GGGCCC 1 cut(s) 1917
Bsp1286I GDGCHC 5 cut(s) 68, 921, 1405, 1921, 2013
Bsp143I GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
BspACI CCGC 3 cut(s) 363, 1033, 1266
BspANI GGCC 5 cut(s) 329, 701, 824, 1388, 1919
BspCNI CTCAG 2 cut(s) 288, 1620
BspDI ATCGAT 1 cut(s) 1971
BspHI TCATGA 1 cut(s) 981
BspLI GGNNCC 7 cut(s) 9, 35, 510, 1498, 1573, 1919, 2095
BspMAI CTGCAG 1 cut(s) 1720
BspPI GGATC 1 cut(s) 409
BspQI GCTCTTC 1 cut(s) 2001
BspT107I GGYRCC 2 cut(s) 33, 1571
BspTI CTTAAG 1 cut(s) 1058
BsrDI GCAATG 3 cut(s) 432, 810, 1672
BsrI ACTGG 4 cut(s) 204, 759, 1592, 1901
BssECI CCNNGG 8 cut(s) 809, 1071, 1129, 1477, 1795, 1921, 2014, 2089
BssMI GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
BssSI CACGAG 1 cut(s) 1404
BssT1I CCWWGG 7 cut(s) 809, 1071, 1129, 1795, 1921, 2014, 2089
Bst2BI CACGAG 1 cut(s) 1404
Bst2UI CCWGG 4 cut(s) 110, 243, 287, 1997
Bst4CI ACNGT 7 cut(s) 659, 820, 1253, 1344, 1369, 1896, 2028
Bst6I CTCTTC 4 cut(s) 501, 1366, 1891, 2001
BstAFI CTTAAG 1 cut(s) 1058
BstAPI GCANNNNNTGC 1 cut(s) 2030
BstC8I GCNNGC 2 cut(s) 234, 327
BstDEI CTNAG 3 cut(s) 296, 1628, 2109
BstF5I GGATG 4 cut(s) 884, 1035, 1125, 1746
BstHHI GCGC 2 cut(s) 1470, 1873
BstKTI GATC 9 cut(s) 417, 925, 1149, 1231, 1258, 1737, 1884, 1980, 2096
BstMAI GTCTC 2 cut(s) 425, 2074
BstMBI GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
BstMWI GCNNNNNNNGC 5 cut(s) 601, 727, 1272, 1832, 2030
BstNI CCWGG 4 cut(s) 110, 243, 287, 1997
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 5 cut(s) 108, 241, 285, 335, 1995
BstSFI CTRYAG 4 cut(s) 1023, 1471, 1716, 1725
BstSLI GKGCMC 1 cut(s) 1921
BstV2I GAAGAC 2 cut(s) 239, 1704
BstX2I RGATCY 3 cut(s) 414, 1881, 2093
BstXI CCANNNNNNTGG 1 cut(s) 1322
BstYI RGATCY 3 cut(s) 414, 1881, 2093
Bsu15I ATCGAT 1 cut(s) 1971
BsuI GTATCC 1 cut(s) 1843
BsuRI GGCC 5 cut(s) 329, 701, 824, 1388, 1919
BsuTUI ATCGAT 1 cut(s) 1971
BtsCI GGATG 4 cut(s) 884, 1035, 1125, 1746
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 7 cut(s) 27, 380, 752, 825, 1249, 1892, 1908
Cac8I GCNNGC 2 cut(s) 234, 327
CciI TCATGA 1 cut(s) 981
CfoI GCGC 2 cut(s) 1470, 1873
Cfr13I GGNCC 6 cut(s) 8, 97, 509, 823, 1917, 1918
ClaI ATCGAT 1 cut(s) 1971
CsiI ACCWGGT 2 cut(s) 241, 285
Csp6I GTAC 3 cut(s) 34, 928, 1572
CspCI CAANNNNNGTGG 2 cut(s) 741, 776
CviAII CATG 5 cut(s) 233, 697, 717, 982, 1144
CviQI GTAC 3 cut(s) 34, 928, 1572
DdeI CTNAG 3 cut(s) 296, 1628, 2109
DpnI GATC 9 cut(s) 416, 924, 1148, 1230, 1257, 1736, 1883, 1979, 2095
DpnII GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
DrdI GACNNNNNNGTC 1 cut(s) 1651
DseDI GACNNNNNNGTC 1 cut(s) 1651
EaeI YGGCCR 2 cut(s) 327, 699
Eam1104I CTCTTC 4 cut(s) 501, 1366, 1891, 2001
EarI CTCTTC 4 cut(s) 501, 1366, 1891, 2001
Ecl136II GAGCTC 1 cut(s) 66
Eco130I CCWWGG 7 cut(s) 809, 1071, 1129, 1795, 1921, 2014, 2089
Eco24I GRGCYC 3 cut(s) 68, 1405, 1921
Eco47I GGWCC 3 cut(s) 8, 97, 509
Eco53kI GAGCTC 1 cut(s) 66
Eco57I CTGAAG 3 cut(s) 393, 426, 611
Eco88I CYCGRG 2 cut(s) 1478, 1538
EcoICRI GAGCTC 1 cut(s) 66
EcoO109I RGGNCCY 4 cut(s) 8, 97, 509, 1918
EcoRII CCWGG 4 cut(s) 108, 241, 285, 1995
EcoT14I CCWWGG 7 cut(s) 809, 1071, 1129, 1795, 1921, 2014, 2089
EcoT38I GRGCYC 3 cut(s) 68, 1405, 1921
ErhI CCWWGG 7 cut(s) 809, 1071, 1129, 1795, 1921, 2014, 2089
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 5 cut(s) 236, 700, 720, 985, 1147
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 1 cut(s) 2032
FatI CATG 5 cut(s) 232, 696, 716, 981, 1143
FauI CCCGC 1 cut(s) 370
FbaI TGATCA 1 cut(s) 1734
FokI GGATG 4 cut(s) 891, 1022, 1112, 1733
FriOI GRGCYC 3 cut(s) 68, 1405, 1921
FspBI CTAG 2 cut(s) 534, 1617
FspI TGCGCA 1 cut(s) 1872
GlaI GCGC 2 cut(s) 1469, 1872
GsaI CCCAGC 1 cut(s) 1918
HaeIII GGCC 5 cut(s) 329, 701, 824, 1388, 1919
HapII CCGG 2 cut(s) 337, 1439
HhaI GCGC 2 cut(s) 1470, 1873
Hin1II CATG 5 cut(s) 236, 700, 720, 985, 1147
Hin6I GCGC 2 cut(s) 1468, 1871
HinP1I GCGC 2 cut(s) 1468, 1871
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 154, 1699
HinfI GANTC 6 cut(s) 162, 744, 784, 1520, 1541, 2000
HpaII CCGG 2 cut(s) 337, 1439
HphI GGTGA 5 cut(s) 926, 1160, 1421, 1433, 1777
Hpy166II GTNNAC 6 cut(s) 148, 292, 541, 1249, 1644, 1768
Hpy188I TCNGA 5 cut(s) 373, 783, 1595, 1755, 2005
Hpy188III TCNNGA 4 cut(s) 251, 476, 982, 1195
Hpy8I GTNNAC 6 cut(s) 148, 292, 541, 1249, 1644, 1768
Hpy99I CGWCG 1 cut(s) 1554
HpyAV CCTTC 5 cut(s) 136, 378, 401, 939, 1760
HpyCH4III ACNGT 7 cut(s) 659, 820, 1253, 1344, 1369, 1896, 2028
HpyCH4IV ACGT 2 cut(s) 543, 1770
HpyF10VI GCNNNNNNNGC 5 cut(s) 601, 727, 1272, 1832, 2030
HpyF3I CTNAG 3 cut(s) 296, 1628, 2109
HpySE526I ACGT 2 cut(s) 543, 1770
Hsp92II CATG 5 cut(s) 236, 700, 720, 985, 1147
HspAI GCGC 2 cut(s) 1468, 1871
KpnI GGTACC 2 cut(s) 37, 1575
Ksp22I TGATCA 1 cut(s) 1734
Kzo9I GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
LguI GCTCTTC 1 cut(s) 2001
LmnI GCTCC 2 cut(s) 552, 1502
LweI GCATC 6 cut(s) 322, 883, 1044, 1244, 1455, 2064
MabI ACCWGGT 2 cut(s) 241, 285
MaeI CTAG 2 cut(s) 534, 1617
MaeII ACGT 2 cut(s) 543, 1770
MaeIII GTNAC 5 cut(s) 53, 515, 850, 951, 1742
MalI GATC 9 cut(s) 416, 924, 1148, 1230, 1257, 1736, 1883, 1979, 2095
MboI GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
MboII GAAGA 8 cut(s) 239, 386, 488, 1353, 1427, 1704, 1878, 2018
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 3 cut(s) 414, 1881, 2093
MhlI GDGCHC 5 cut(s) 68, 921, 1405, 1921, 2013
MlyI GAGTC 1 cut(s) 1550
MmeI TCCRAC 1 cut(s) 1827
MseI TTAA 8 cut(s) 182, 275, 624, 686, 831, 1059, 1559, 1659
MslI CAYNNNNRTG 2 cut(s) 963, 1040
MspA1I CMGCKG 2 cut(s) 680, 1160
MspCI CTTAAG 1 cut(s) 1058
MspI CCGG 2 cut(s) 337, 1439
MspR9I CCNGG 5 cut(s) 110, 243, 287, 337, 1997
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 2 cut(s) 1275, 1604
MvaI CCWGG 4 cut(s) 110, 243, 287, 1997
MwoI GCNNNNNNNGC 5 cut(s) 601, 727, 1272, 1832, 2030
NciI CCSGG 1 cut(s) 337
NdeII GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
NlaIII CATG 5 cut(s) 236, 700, 720, 985, 1147
NlaIV GGNNCC 7 cut(s) 9, 35, 510, 1498, 1573, 1919, 2095
NmuCI GTSAC 2 cut(s) 53, 850
NsbI TGCGCA 1 cut(s) 1872
NspI RCATGY 1 cut(s) 236
PaeI GCATGC 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 1538
PagI TCATGA 1 cut(s) 981
PciSI GCTCTTC 1 cut(s) 2001
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PctI GAATGC 2 cut(s) 1275, 1604
PfeI GAWTC 5 cut(s) 162, 744, 784, 1520, 2000
PflMI CCANNNNNTGG 1 cut(s) 911
PleI GAGTC 1 cut(s) 1549
PpsI GAGTC 1 cut(s) 1549
PpuMI RGGWCCY 3 cut(s) 8, 97, 509
PshBI ATTAAT 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 68
Psp5II RGGWCCY 3 cut(s) 8, 97, 509
Psp6I CCWGG 4 cut(s) 108, 241, 285, 1995
PspFI CCCAGC 1 cut(s) 1914
PspGI CCWGG 4 cut(s) 108, 241, 285, 1995
PspN4I GGNNCC 7 cut(s) 9, 35, 510, 1498, 1573, 1919, 2095
PspOMI GGGCCC 1 cut(s) 1917
PspPI GGNCC 6 cut(s) 8, 97, 509, 823, 1917, 1918
PspPPI RGGWCCY 3 cut(s) 8, 97, 509
PspXI VCTCGAGB 1 cut(s) 1538
PstI CTGCAG 1 cut(s) 1720
PsuI RGATCY 3 cut(s) 414, 1881, 2093
PvuII CAGCTG 2 cut(s) 680, 1160
RsaI GTAC 3 cut(s) 35, 929, 1573
RsaNI GTAC 3 cut(s) 34, 928, 1572
RseI CAYNNNNRTG 2 cut(s) 963, 1040
SacI GAGCTC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 2001
SaqAI TTAA 8 cut(s) 182, 275, 624, 686, 831, 1059, 1559, 1659
Sau3AI GATC 9 cut(s) 414, 922, 1146, 1228, 1255, 1734, 1881, 1977, 2093
Sau96I GGNCC 6 cut(s) 8, 97, 509, 823, 1917, 1918
SchI GAGTC 1 cut(s) 1550
ScrFI CCNGG 5 cut(s) 110, 243, 287, 337, 1997
SduI GDGCHC 5 cut(s) 68, 921, 1405, 1921, 2013
SexAI ACCWGGT 2 cut(s) 241, 285
SfaNI GCATC 6 cut(s) 322, 883, 1044, 1244, 1455, 2064
SfcI CTRYAG 4 cut(s) 1023, 1471, 1716, 1725
Sfr274I CTCGAG 1 cut(s) 1538
SinI GGWCC 3 cut(s) 8, 97, 509
SlaI CTCGAG 1 cut(s) 1538
SmiMI CAYNNNNRTG 2 cut(s) 963, 1040
SmlI CTYRAG 4 cut(s) 151, 1058, 1089, 1538
SmoI CTYRAG 4 cut(s) 151, 1058, 1089, 1538
SphI GCATGC 1 cut(s) 236
SsiI CCGC 3 cut(s) 363, 1033, 1266
SspI AATATT 4 cut(s) 269, 622, 835, 1104
SspMI CTAG 2 cut(s) 534, 1617
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 5 cut(s) 108, 241, 285, 335, 1995
StyI CCWWGG 7 cut(s) 809, 1071, 1129, 1795, 1921, 2014, 2089
TaaI ACNGT 7 cut(s) 659, 820, 1253, 1344, 1369, 1896, 2028
TaiI ACGT 2 cut(s) 546, 1773
TaqI TCGA 6 cut(s) 258, 321, 1211, 1539, 1971, 2049
TaqII GACCGA 1 cut(s) 663
TauI GCSGC 1 cut(s) 1035
TfiI GAWTC 5 cut(s) 162, 744, 784, 1520, 2000
Tru1I TTAA 8 cut(s) 182, 275, 624, 686, 831, 1059, 1559, 1659
Tru9I TTAA 8 cut(s) 182, 275, 624, 686, 831, 1059, 1559, 1659
TscAI CASTG 7 cut(s) 27, 387, 759, 825, 1256, 1899, 1908
TseFI GTSAC 2 cut(s) 53, 850
Tsp45I GTSAC 2 cut(s) 53, 850
TspDTI ATGAA 4 cut(s) 443, 1268, 1469, 1977
TspGWI ACGGA 4 cut(s) 407, 564, 915, 953
TspRI CASTG 7 cut(s) 27, 387, 759, 825, 1256, 1899, 1908
Van91I CCANNNNNTGG 1 cut(s) 911
Vha464I CTTAAG 1 cut(s) 1058
VpaK11BI GGWCC 3 cut(s) 8, 97, 509
VspI ATTAAT 1 cut(s) 624
XceI RCATGY 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 1538
XspI CTAG 2 cut(s) 534, 1617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.