MD03G1225900.v1.1

Rare lipoprotein A (RlpA)-like double-psi beta-barrel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014349
Physical Location & Seq
Reverse (-)
31137983 .. 31139962
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1225900.v1.1.491

Sequence Viewer

Length: 708 bp
ATGTATAGAGAGAGAATTAAAATGGGGCATAGTCTTGCAGTTTCTTGTGCTTTTTTCTGGAATATTTTCTTGTTTGCTATCACCAATGTTGAAGCCAATGGATGGCTTAGTGGTCATGCAACTTTCTATGGGACCAACCAGGACCCAACTACCCTTGGTGGTGCTTGTGGTTATGACAGTACCATCCACGCGGGTTTTGGAGTGAATACAGCGGCCGTGAGTAGCACACTTTTCAGAGGAGGCGAGGCGTGTGGGGCTTGTTACCAAGTAATGTGCAACTACAACACAGATCCGAAGTGGTGCCTTAGAGGTCGTTCTGTGACAGTTTCCGCCACCAATTTCTGCCCCCCAAACAACAACGGAGGGTGGGTATCGTGTAAAAGAAGAGGCGGAGTCCGATTCACCTTGAAGGGACAGTCAAGTTTCAACATGGTGATGATCTCCAACGTCGGGGGAAGCGGTGACGTGAAGGCTGCATGGATTAGAGGGTCGAGGATGAGAAGAGGAACGTGGCTGCCCATGCACAGGAACTGGGGTGCAAATTGGCAAAGCAGCATCGACCTTCGAAACCAGAGACTCTCGTTTAAGCTCACATTGATTGATGGAAGAGCAATTGTATTTTCTAATGTTGTTCCTTCCACTTGGGGGGTTTGGGCAAACATTTTCATCCCGCAACCAGTTCTGATCTTACGTACCATTCATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

236

Amino Acids

25.93

Weight (kDa)

10.01

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 41 - 127 5.9e-07 EXPB1-like domain 1
DPBB_1 PF03330 70 - 119 2.2e-11 Lytic transglycolase
Expansin_C PF01357 132 - 212 1.8e-25 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15370 AT3G15370
fragaria_vesca FvH4_3g20110
malus_domestica MD03G1225900.v1.1 MD11G1245100.v1.1 MD11G1245500.v1.1
prunus_persica Prupe.4G183400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0033911
rosa_laevigata RLG00000033510
rosa_multiflora Rmu_sc0001150.1_g000013 Rmu_sc0016967.1_g000001
rosa_rugosa Rorug05G0143300.1
rosa_samantha Rh5AG234100 Rh5BG234700 Rh5CG265000 Rh5DG241600
rosa_wichuraiana Rw5G021520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 300
AccB7I CCANNNNNTGG 1 cut(s) 102
AccII CGCG 1 cut(s) 191
AciI CCGC 6 cut(s) 191, 212, 330, 390, 459, 671
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 1 cut(s) 213
AfaI GTAC 2 cut(s) 181, 694
AfiI CCNNNNNNNGG 4 cut(s) 102, 450, 525, 645
AgsI TTSAA 3 cut(s) 92, 409, 427
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 1 cut(s) 589
AluI AGCT 1 cut(s) 589
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 1 cut(s) 284
AlwNI CAGNNNCTG 1 cut(s) 531
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 3 cut(s) 473, 514, 552
Asp700I GAANNNNTTC 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 132, 142
AsuHPI GGTGA 4 cut(s) 73, 394, 445, 473
AsuII TTCGAA 1 cut(s) 565
AvaII GGWCC 2 cut(s) 132, 142
BanI GGYRCC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 460, 501, 564
BccI CCATC 3 cut(s) 96, 191, 596
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 568
BisI GCNGC 4 cut(s) 213, 474, 515, 553
BlsI GCNGC 4 cut(s) 214, 475, 516, 554
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 2 cut(s) 132, 142
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 2 cut(s) 132, 142
BmiI GGNNCC 3 cut(s) 133, 144, 302
BmrFI CCNGG 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 541
BmsI GCATC 1 cut(s) 564
BmuI ACTGGG 1 cut(s) 541
Bpu14I TTCGAA 1 cut(s) 565
BsaAI YACGTR 1 cut(s) 692
BsaJI CCNNGG 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 450, 525, 645
Bse1I ACTGG 2 cut(s) 536, 677
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 4 cut(s) 107, 183, 501, 666
BseLI CCNNNNNNNGG 4 cut(s) 102, 450, 525, 645
BseNI ACTGG 2 cut(s) 536, 677
BseRI GAGGAG 1 cut(s) 252
BseX3I CGGCCG 1 cut(s) 213
BseXI GCAGC 3 cut(s) 460, 501, 564
Bsh1236I CGCG 1 cut(s) 191
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 1 cut(s) 215
BshNI GGYRCC 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 216
BslFI GGGAC 2 cut(s) 145, 426
BslI CCNNNNNNNGG 4 cut(s) 102, 450, 525, 645
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 2 cut(s) 145, 426
BsnI GGCC 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 565
Bsp143I GATC 3 cut(s) 289, 438, 684
BspACI CCGC 6 cut(s) 191, 212, 330, 390, 459, 671
BspANI GGCC 1 cut(s) 215
BspFNI CGCG 1 cut(s) 191
BspLI GGNNCC 3 cut(s) 133, 144, 302
BspPI GGATC 1 cut(s) 284
BspQI GCTCTTC 1 cut(s) 601
BspT104I TTCGAA 1 cut(s) 565
BspT107I GGYRCC 1 cut(s) 300
BsrI ACTGG 2 cut(s) 536, 677
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 289, 438, 684
BssT1I CCWWGG 1 cut(s) 154
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 179, 325, 417
Bst6I CTCTTC 3 cut(s) 379, 496, 601
BstBAI YACGTR 1 cut(s) 692
BstBI TTCGAA 1 cut(s) 565
BstDEI CTNAG 2 cut(s) 107, 305
BstF5I GGATG 4 cut(s) 107, 183, 501, 666
BstFNI CGCG 1 cut(s) 191
BstKTI GATC 3 cut(s) 292, 441, 687
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 3 cut(s) 289, 438, 684
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 2 cut(s) 254, 520
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSNI TACGTA 1 cut(s) 692
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 3 cut(s) 460, 501, 564
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BstZI CGGCCG 1 cut(s) 213
BsuRI GGCC 1 cut(s) 215
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 4 cut(s) 107, 183, 501, 666
CaiI CAGNNNCTG 1 cut(s) 531
Cfr13I GGNCC 2 cut(s) 132, 142
Csp6I GTAC 2 cut(s) 180, 693
CviAII CATG 4 cut(s) 116, 430, 477, 520
CviJI RGCY 7 cut(s) 95, 106, 215, 257, 473, 514, 589
CviKI_1 RGCY 7 cut(s) 95, 106, 215, 257, 473, 514, 589
CviQI GTAC 2 cut(s) 180, 693
DdeI CTNAG 2 cut(s) 107, 305
DpnI GATC 3 cut(s) 291, 440, 686
DpnII GATC 3 cut(s) 289, 438, 684
EaeI YGGCCR 1 cut(s) 213
EagI CGGCCG 1 cut(s) 213
Eam1104I CTCTTC 3 cut(s) 379, 496, 601
EarI CTCTTC 3 cut(s) 379, 496, 601
EciI GGCGGA 2 cut(s) 319, 405
EclXI CGGCCG 1 cut(s) 213
Eco105I TACGTA 1 cut(s) 692
Eco130I CCWWGG 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 132, 142
Eco52I CGGCCG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 4 cut(s) 119, 433, 480, 523
FaiI YATR 8 cut(s) 6, 30, 117, 129, 174, 431, 478, 521
FaqI GGGAC 2 cut(s) 145, 426
FatI CATG 4 cut(s) 115, 429, 476, 519
FauI CCCGC 2 cut(s) 184, 678
Fnu4HI GCNGC 4 cut(s) 213, 474, 515, 553
FokI GGATG 4 cut(s) 114, 170, 508, 653
Fsp4HI GCNGC 4 cut(s) 213, 474, 515, 553
GluI GCNGC 4 cut(s) 213, 474, 515, 553
HaeIII GGCC 1 cut(s) 215
Hin1II CATG 4 cut(s) 119, 433, 480, 523
HinfI GANTC 3 cut(s) 393, 399, 576
HphI GGTGA 4 cut(s) 73, 394, 445, 473
Hpy188I TCNGA 4 cut(s) 236, 294, 398, 684
Hpy188III TCNNGA 1 cut(s) 58
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 4 cut(s) 403, 463, 572, 645
HpyCH4III ACNGT 3 cut(s) 179, 325, 417
HpyCH4IV ACGT 4 cut(s) 447, 465, 509, 691
HpyCH4V TGCA 6 cut(s) 38, 119, 276, 476, 523, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 520
HpyF3I CTNAG 2 cut(s) 107, 305
HpySE526I ACGT 4 cut(s) 447, 465, 509, 691
Hsp92II CATG 4 cut(s) 119, 433, 480, 523
Kzo9I GATC 3 cut(s) 289, 438, 684
LguI GCTCTTC 1 cut(s) 601
LpnPI CCDG 7 cut(s) 43, 125, 152, 511, 517, 584, 690
Lsp1109I GCAGC 3 cut(s) 460, 501, 564
LweI GCATC 1 cut(s) 564
MaeII ACGT 4 cut(s) 447, 465, 509, 691
MaeIII GTNAC 3 cut(s) 260, 319, 461
MalI GATC 3 cut(s) 291, 440, 686
MboI GATC 3 cut(s) 289, 438, 684
MboII GAAGA 3 cut(s) 396, 513, 618
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 289
MluCI AATT 4 cut(s) 15, 337, 541, 612
MlyI GAGTC 2 cut(s) 402, 570
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 9 cut(s) 230, 233, 238, 302, 356, 380, 479, 486, 497
MroXI GAANNNNTTC 1 cut(s) 65
MseI TTAA 3 cut(s) 18, 585, 706
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 140
MunI CAATTG 1 cut(s) 612
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 254, 520
NdeII GATC 3 cut(s) 289, 438, 684
NlaIII CATG 4 cut(s) 119, 433, 480, 523
NlaIV GGNNCC 3 cut(s) 133, 144, 302
NmuCI GTSAC 2 cut(s) 319, 461
NspV TTCGAA 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 601
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 399
PflMI CCANNNNNTGG 1 cut(s) 102
PkrI GCNGC 4 cut(s) 214, 475, 516, 554
PleI GAGTC 2 cut(s) 401, 570
PpsI GAGTC 2 cut(s) 401, 570
Ppu21I YACGTR 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 133, 144, 302
PspPI GGNCC 2 cut(s) 132, 142
PspPPI RGGWCCY 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 531
PsuI RGATCY 1 cut(s) 289
RsaI GTAC 2 cut(s) 181, 694
RsaNI GTAC 2 cut(s) 180, 693
RseI CAYNNNNRTG 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 601
SaqAI TTAA 3 cut(s) 18, 585, 706
SatI GCNGC 4 cut(s) 213, 474, 515, 553
Sau3AI GATC 3 cut(s) 289, 438, 684
Sau96I GGNCC 2 cut(s) 132, 142
SchI GAGTC 2 cut(s) 402, 570
ScrFI CCNGG 1 cut(s) 140
SetI ASST 8 cut(s) 313, 407, 450, 468, 512, 564, 591, 694
SfaNI GCATC 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 565
SinI GGWCC 2 cut(s) 132, 142
SmiMI CAYNNNNRTG 1 cut(s) 434
SnaBI TACGTA 1 cut(s) 692
Sse9I AATT 4 cut(s) 15, 337, 541, 612
SsiI CCGC 6 cut(s) 191, 212, 330, 390, 459, 671
SspI AATATT 1 cut(s) 64
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 179, 325, 417
TaiI ACGT 4 cut(s) 450, 468, 512, 694
TaqI TCGA 3 cut(s) 491, 558, 565
TasI AATT 4 cut(s) 15, 337, 541, 612
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 399
Tru1I TTAA 3 cut(s) 18, 585, 706
Tru9I TTAA 3 cut(s) 18, 585, 706
TseFI GTSAC 2 cut(s) 319, 461
TseI GCWGC 3 cut(s) 473, 514, 552
Tsp45I GTSAC 2 cut(s) 319, 461
TspDTI ATGAA 2 cut(s) 655, 689
TspGWI ACGGA 1 cut(s) 375
Van91I CCANNNNNTGG 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 132, 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.