RchiOBHm_Chr5g0033911

Rare lipoprotein A (RlpA)-like double-psi beta-barrel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014349
Physical Location & Seq
Reverse (-)
27797365 .. 27799426
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31295

Sequence Viewer

Length: 765 bp
ATGAAAGCTGCAGGTTCTTTCTGTGTTCTTTTTTGGCAGATTATCTTCTTTGTTAGTGTAGTTTTGGAGGGCATCAATGGCGCTCAAGCTAATGGTTGGCTTAACGCTCATGCAACTTTCTATGGGGTCAACCAGGACCCTGCCACCCTTGGAGGTGCGTGTGGTTATGGAAATACATTAATGCTTGCGGGGTTCGGAGTGAACACAGCAGCAGTTAGTGGCATACTTTTCAGAGGTGGCGAGGCATGTGGTGCTTGTTACCAAGTGATGTGCAACTATAAGTTGGATCCAAAGTGGTGCCTTCAACGACGAGCCGTGACTGTATCAGCCACCAATTTCTGCCCGCCAAACAACAATGGAGGGTGGTGCAATCCCCCTCACCACCACTTCGACATGTCTATGCCGGCGTTTCTTCTCATTGCACGACAAGGAAATGAAGGCATTGTTCCTGTGATCTACAGAAGAGGAGGAGTCCGATTCACGTTGAAAGGGCAATCGAGTTTTAACATGGTGATGATATCGAATGTTGGCGGAAGCGGCGCCGCGAAGGGGGCGTGGATAAGGGTGTCGAGGATGAGAAGCGGCACATGGATACCGATGCATAGGAATTGGGGTGCGAATTGGCAAAGCAGCATCGACCTGAGAAATCAACAACTTTCTTTCAAGCTCACTTTGATTGATGGAAAAGCCCTAGTGTTTTACAACGTTGTTCCTTCCACTTGGAGATTTGGACAAACATTTTCATCCCAGAACCAGTTCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

254

Amino Acids

27.8

Weight (kDa)

9.69

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 38 - 126 2.5e-07 EXPB1-like domain 1
DPBB_1 PF03330 69 - 153 1.6e-18 Lytic transglycolase
Expansin_C PF01357 158 - 238 5e-23 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15370 AT3G15370
fragaria_vesca FvH4_3g20110
malus_domestica MD03G1225900.v1.1 MD11G1245100.v1.1 MD11G1245500.v1.1
prunus_persica Prupe.4G183400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0033911
rosa_laevigata RLG00000033510
rosa_multiflora Rmu_sc0001150.1_g000013 Rmu_sc0016967.1_g000001
rosa_rugosa Rorug05G0143300.1
rosa_samantha Rh5AG234100 Rh5BG234700 Rh5CG265000 Rh5DG241600
rosa_wichuraiana Rw5G021520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2
AccB1I GGYRCC 2 cut(s) 297, 539
AccII CGCG 1 cut(s) 545
AciI CCGC 6 cut(s) 188, 344, 531, 537, 543, 582
AclI AACGTT 1 cut(s) 705
AclWI GGATC 2 cut(s) 281, 294
AcyI GRCGYC 1 cut(s) 540
AfiI CCNNNNNNNGG 1 cut(s) 549
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 3 cut(s) 305, 487, 664
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 3 cut(s) 8, 89, 667
AluI AGCT 3 cut(s) 8, 89, 667
AlwI GGATC 2 cut(s) 281, 294
ApeKI GCWGC 3 cut(s) 8, 209, 630
AseI ATTAAT 1 cut(s) 179
Asp700I GAANNNNTTC 1 cut(s) 755
AspLEI GCGC 2 cut(s) 83, 542
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 371, 523
AvaII GGWCC 1 cut(s) 136
BamHI GGATCC 1 cut(s) 286
BanI GGYRCC 2 cut(s) 297, 539
BbvI GCAGC 2 cut(s) 221, 642
BccI CCATC 1 cut(s) 674
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 585
BfaI CTAG 1 cut(s) 692
BfmI CTRYAG 2 cut(s) 9, 457
BfoI RGCGCY 2 cut(s) 84, 543
BfuAI ACCTGC 1 cut(s) 2
BfuI GTATCC 1 cut(s) 585
BisI GCNGC 6 cut(s) 9, 210, 538, 543, 583, 631
BlsI GCNGC 6 cut(s) 10, 211, 539, 544, 584, 632
Bme1390I CCNGG 1 cut(s) 134
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 4 cut(s) 138, 288, 299, 541
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 3 cut(s) 81, 588, 642
BpuEI CTTGAG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 540
BsaJI CCNNGG 1 cut(s) 148
Bsc4I CCNNNNNNNGG 1 cut(s) 549
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 754
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 2 cut(s) 579, 743
BseLI CCNNNNNNNGG 1 cut(s) 549
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 632
BseNI ACTGG 1 cut(s) 754
BseRI GAGGAG 2 cut(s) 480, 483
BseXI GCAGC 2 cut(s) 221, 642
Bsh1236I CGCG 1 cut(s) 545
BshNI GGYRCC 2 cut(s) 297, 539
BsiSI CCGG 1 cut(s) 404
BslI CCNNNNNNNGG 1 cut(s) 549
Bsp143I GATC 2 cut(s) 286, 453
BspACI CCGC 6 cut(s) 188, 344, 531, 537, 543, 582
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 4 cut(s) 138, 288, 299, 541
BspMAI CTGCAG 1 cut(s) 13
BspMI ACCTGC 1 cut(s) 2
BspPI GGATC 2 cut(s) 281, 294
BspT107I GGYRCC 2 cut(s) 297, 539
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 403
BsrI ACTGG 1 cut(s) 754
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 2 cut(s) 286, 453
BssNI GRCGYC 1 cut(s) 540
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 1 cut(s) 457
BstACI GRCGYC 1 cut(s) 540
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 3 cut(s) 186, 344, 405
BstDEI CTNAG 2 cut(s) 641, 762
BstF5I GGATG 2 cut(s) 579, 743
BstFNI CGCG 1 cut(s) 545
BstH2I RGCGCY 2 cut(s) 84, 543
BstHHI GCGC 2 cut(s) 83, 542
BstKTI GATC 2 cut(s) 289, 456
BstMBI GATC 2 cut(s) 286, 453
BstMWI GCNNNNNNNGC 4 cut(s) 78, 251, 537, 551
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 2 cut(s) 249, 397
BstSCI CCNGG 1 cut(s) 132
BstSFI CTRYAG 2 cut(s) 9, 457
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 2 cut(s) 221, 642
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuI GTATCC 1 cut(s) 585
BtsCI GGATG 2 cut(s) 579, 743
BveI ACCTGC 1 cut(s) 2
Cac8I GCNNGC 3 cut(s) 186, 344, 405
CfoI GCGC 2 cut(s) 83, 542
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 136
CviAII CATG 5 cut(s) 110, 246, 394, 508, 588
CviJI RGCY 7 cut(s) 8, 89, 100, 314, 329, 667, 689
CviKI_1 RGCY 7 cut(s) 8, 89, 100, 314, 329, 667, 689
DdeI CTNAG 2 cut(s) 641, 762
DinI GGCGCC 1 cut(s) 541
DpnI GATC 2 cut(s) 288, 455
DpnII GATC 2 cut(s) 286, 453
Eam1104I CTCTTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 457
EciI GGCGGA 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 148
Eco32I GATATC 1 cut(s) 519
Eco47I GGWCC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 519
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 603
EgeI GGCGCC 1 cut(s) 541
EheI GGCGCC 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 5 cut(s) 113, 249, 397, 511, 591
FatI CATG 5 cut(s) 109, 245, 393, 507, 587
FauI CCCGC 2 cut(s) 181, 351
Fnu4HI GCNGC 6 cut(s) 9, 210, 538, 543, 583, 631
FokI GGATG 2 cut(s) 586, 730
Fsp4HI GCNGC 6 cut(s) 9, 210, 538, 543, 583, 631
FspBI CTAG 1 cut(s) 692
GlaI GCGC 2 cut(s) 82, 541
GluI GCNGC 6 cut(s) 9, 210, 538, 543, 583, 631
HaeII RGCGCY 2 cut(s) 84, 543
HapII CCGG 1 cut(s) 404
HhaI GCGC 2 cut(s) 83, 542
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 5 cut(s) 113, 249, 397, 511, 591
Hin6I GCGC 2 cut(s) 81, 540
HinP1I GCGC 2 cut(s) 81, 540
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 2 cut(s) 471, 477
HpaII CCGG 1 cut(s) 404
HphI GGTGA 2 cut(s) 371, 523
Hpy166II GTNNAC 2 cut(s) 130, 202
Hpy188I TCNGA 3 cut(s) 197, 233, 476
Hpy8I GTNNAC 2 cut(s) 130, 202
Hpy99I CGWCG 1 cut(s) 312
HpyAV CCTTC 4 cut(s) 311, 431, 541, 723
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 2 cut(s) 482, 705
HpyCH4V TGCA 6 cut(s) 11, 113, 273, 369, 422, 601
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 251, 537, 551
HpyF3I CTNAG 2 cut(s) 641, 762
HpySE526I ACGT 2 cut(s) 482, 705
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 5 cut(s) 113, 249, 397, 511, 591
HspAI GCGC 2 cut(s) 81, 540
KasI GGCGCC 1 cut(s) 539
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
Kzo9I GATC 2 cut(s) 286, 453
LpnPI CCDG 7 cut(s) 119, 146, 153, 417, 462, 653, 761
Lsp1109I GCAGC 2 cut(s) 221, 642
LweI GCATC 3 cut(s) 81, 588, 642
MaeI CTAG 1 cut(s) 692
MaeII ACGT 2 cut(s) 482, 705
MaeIII GTNAC 2 cut(s) 257, 316
MalI GATC 2 cut(s) 288, 455
MboI GATC 2 cut(s) 286, 453
MboII GAAGA 3 cut(s) 37, 404, 474
MflI RGATCY 1 cut(s) 286
MluCI AATT 3 cut(s) 334, 607, 619
Mly113I GGCGCC 1 cut(s) 540
MlyI GAGTC 1 cut(s) 480
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 9 cut(s) 61, 146, 227, 235, 353, 387, 458, 461, 564
Mph1103I ATGCAT 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 755
MseI TTAA 3 cut(s) 102, 179, 504
MslI CAYNNNNRTG 2 cut(s) 398, 512
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 1 cut(s) 134
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 4 cut(s) 78, 251, 537, 551
NaeI GCCGGC 1 cut(s) 405
NarI GGCGCC 1 cut(s) 540
NdeII GATC 2 cut(s) 286, 453
NgoMIV GCCGGC 1 cut(s) 403
NlaIII CATG 5 cut(s) 113, 249, 397, 511, 591
NlaIV GGNNCC 4 cut(s) 138, 288, 299, 541
NmuCI GTSAC 1 cut(s) 316
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 2 cut(s) 249, 397
PciI ACATGT 1 cut(s) 393
PdiI GCCGGC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 1 cut(s) 477
PkrI GCNGC 6 cut(s) 10, 211, 539, 544, 584, 632
PleI GAGTC 1 cut(s) 479
PluTI GGCGCC 1 cut(s) 543
PpsI GAGTC 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 393
PshBI ATTAAT 1 cut(s) 179
Psp1406I AACGTT 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 4 cut(s) 138, 288, 299, 541
PspPI GGNCC 1 cut(s) 136
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 13
PsuI RGATCY 1 cut(s) 286
RseI CAYNNNNRTG 2 cut(s) 398, 512
SaqAI TTAA 3 cut(s) 102, 179, 504
SatI GCNGC 6 cut(s) 9, 210, 538, 543, 583, 631
Sau3AI GATC 2 cut(s) 286, 453
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 480
ScrFI CCNGG 1 cut(s) 134
SetI ASST 9 cut(s) 10, 16, 91, 157, 238, 485, 642, 669, 708
SfaNI GCATC 3 cut(s) 81, 588, 642
SfcI CTRYAG 2 cut(s) 9, 457
SfoI GGCGCC 1 cut(s) 541
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 398, 512
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 3 cut(s) 334, 607, 619
SsiI CCGC 6 cut(s) 188, 344, 531, 537, 543, 582
SspDI GGCGCC 1 cut(s) 539
SspMI CTAG 1 cut(s) 692
StyD4I CCNGG 1 cut(s) 132
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 2 cut(s) 485, 708
TaqI TCGA 5 cut(s) 390, 497, 521, 569, 636
TasI AATT 3 cut(s) 334, 607, 619
TauI GCSGC 3 cut(s) 540, 545, 585
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 3 cut(s) 102, 179, 504
Tru9I TTAA 3 cut(s) 102, 179, 504
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 8, 209, 630
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 3 cut(s) 17, 450, 732
VpaK11BI GGWCC 1 cut(s) 136
VspI ATTAAT 1 cut(s) 179
XceI RCATGY 2 cut(s) 249, 397
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 1 cut(s) 692
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.