Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0016289

Physical Location & Seq

Genomic Coordinates Forward (+)

31208052 .. 31208711

Gene Structure 660 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1226900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 660 bp

ATGATTTCTAATCCCAATATTCTTCGTCAATCTTCTCACAATCATCTCAGTTTTTTGTTGTGCATTTTTGCGCTCGGAATAGTAGGTTTCTTGTTTCTTCTAGGCCAAGCGAGATTCACGAATCTCAGCCGCCTTGTAGTTTGGATCGGCAACTTAGCGCTCTGCACTGCGGCTTTGTTTCTCATTGCCTTGATGCATCCCATTATTGGAATCATATTTTCTTGGCTTCCAAACAGAAGCCATAGCGATCGTGAATTTGAAGGAGCAGACGACCTGTCTCACCACCATCATGATCAAGATACATTACCACAAGCACCTGCGCAAGATATTGCAACGTTGTTCCACATGGTGTTAATGGAAGTGCTCGATAGAGTTTGGGAGGACTTGGTGATGGAGGAGGGGCCAGAACAAATGCCTCAAGCTCTTCAAGTTCAAGCCCTAGAGGCCCTCCCACCGCCTTTGAGGTACTGCACAGGCAGCCGGGACATAACATCGAGTTGCTGCAGTGACCGGTGTACCATTTGCTTGGAGGATTACATGGACGGCGAGTCTTGTCGTGTCTTCGCGTGCAAACACATGTTTCATTCCAATTGTATTGATCATTGGTTAAAGAATCACCTAACTTGCCCAGTTTGTCGAAACTCTGTTGTAGATATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

220

Amino Acids

24.78

Weight (kDa)

5.5

Isoelectric Point (pI)

53.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	169 - 213	4.2e-07	RING-H2 zinc finger domain
zf-RING_2	PF13639	171 - 213	9.6e-14	Ring finger domain
zf-C3HC4_2	PF13923	171 - 212	1.8e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	172 - 199	1.6e-08	RING-like zinc finger
zf-C3HC4	PF00097	172 - 212	2.7e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0016289)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g20220
malus_domestica	MD03G1226900.v1.1 MD11G1244100.v1.1
prunus_persica	Prupe.4G182600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0034041
rosa_laevigata	RLG00000033519
rosa_multiflora	Rmu_co8356837.1_g000001
rosa_roxburghii	Rroxscaffold_1G00046200
rosa_rugosa	Rorug05G0144300
rosa_samantha	Rh5AG235300 Rh5BG236000 Rh5CG265900 Rh5DG242900
rosa_wichuraiana	Rw5G021240 Rw5G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	325
Acc16I	TGCGCA	1 cut(s)	321
Acc36I	ACCTGC	1 cut(s)	325
AccII	CGCG	1 cut(s)	566
AciI	CCGC	3 cut(s)	130, 170, 455
AclI	AACGTT	1 cut(s)	335
AclWI	GGATC	1 cut(s)	152
AcsI	RAATTY	1 cut(s)	254
AdeI	CACNNNGTG	1 cut(s)	349
AfaI	GTAC	2 cut(s)	467, 517
AfeI	AGCGCT	1 cut(s)	159
AfiI	CCNNNNNNNGG	1 cut(s)	206
AflIII	ACRYGT	1 cut(s)	576
AgeI	ACCGGT	1 cut(s)	510
AgsI	TTSAA	3 cut(s)	260, 428, 434
AhdI	GACNNNNNGTC	2 cut(s)	274, 547
AluBI	AGCT	1 cut(s)	422
AluI	AGCT	1 cut(s)	422
Alw21I	GWGCWC	1 cut(s)	366
Alw26I	GTCTC	1 cut(s)	282
AlwI	GGATC	1 cut(s)	152
Aor51HI	AGCGCT	1 cut(s)	159
AoxI	GGCC	3 cut(s)	103, 401, 444
ApeKI	GCWGC	2 cut(s)	477, 501
ApoI	RAATTY	1 cut(s)	254
AsiGI	ACCGGT	1 cut(s)	510
AspLEI	GCGC	3 cut(s)	73, 160, 322
AspS9I	GGNCC	2 cut(s)	401, 445
AsuC2I	CCSGG	1 cut(s)	482
AsuHPI	GGTGA	3 cut(s)	272, 400, 608
BaeI	ACNNNNGTAYC	2 cut(s)	499, 532
BbsI	GAAGAC	1 cut(s)	553
Bbv12I	GWGCWC	1 cut(s)	366
BbvI	GCAGC	2 cut(s)	488, 489
BccI	CCATC	2 cut(s)	294, 385
BceAI	ACGGC	1 cut(s)	559
BclI	TGATCA	2 cut(s)	292, 598
BcnI	CCSGG	1 cut(s)	482
BcoDI	GTCTC	1 cut(s)	282
BfaI	CTAG	2 cut(s)	101, 440
BfmI	CTRYAG	1 cut(s)	502
BfoI	RGCGCY	1 cut(s)	161
BfuAI	ACCTGC	1 cut(s)	325
BglI	GCCNNNNNGGC	1 cut(s)	443
BisI	GCNGC	4 cut(s)	130, 171, 478, 502
BlsI	GCNGC	4 cut(s)	131, 172, 479, 503
Bme1390I	CCNGG	1 cut(s)	482
BmeRI	GACNNNNNGTC	2 cut(s)	274, 547
BmgT120I	GGNCC	2 cut(s)	401, 445
BmiI	GGNNCC	1 cut(s)	402
BmrFI	CCNGG	1 cut(s)	482
BmrI	ACTGGG	1 cut(s)	623
BmsI	GCATC	2 cut(s)	183, 205
BmuI	ACTGGG	1 cut(s)	623
BpiI	GAAGAC	1 cut(s)	553
BpuEI	CTTGAG	1 cut(s)	402
BpuMI	CCSGG	1 cut(s)	482
BsaWI	WCCGGW	1 cut(s)	510
Bsc4I	CCNNNNNNNGG	1 cut(s)	206
Bse118I	RCCGGY	1 cut(s)	510
Bse1I	ACTGG	1 cut(s)	629
Bse3DI	GCAATG	1 cut(s)	183
BseGI	GGATG	1 cut(s)	196
BseLI	CCNNNNNNNGG	1 cut(s)	206
BseMI	GCAATG	1 cut(s)	183
BseMII	CTCAG	2 cut(s)	61, 139
BseNI	ACTGG	1 cut(s)	629
BseRI	GAGGAG	1 cut(s)	410
BseXI	GCAGC	2 cut(s)	488, 489
BsgI	GTGCAG	2 cut(s)	148, 454
Bsh1236I	CGCG	1 cut(s)	566
Bsh1285I	CGRYCG	1 cut(s)	250
BshFI	GGCC	3 cut(s)	105, 403, 446
BshTI	ACCGGT	1 cut(s)	510
BsiEI	CGRYCG	1 cut(s)	250
BsiHKAI	GWGCWC	1 cut(s)	366
BsiSI	CCGG	2 cut(s)	481, 511
BslFI	GGGAC	1 cut(s)	497
BslI	CCNNNNNNNGG	1 cut(s)	206
BsmAI	GTCTC	1 cut(s)	282
BsmFI	GGGAC	1 cut(s)	497
BsnI	GGCC	3 cut(s)	105, 403, 446
Bsp1286I	GDGCHC	1 cut(s)	366
Bsp143I	GATC	4 cut(s)	144, 247, 292, 598
BspACI	CCGC	3 cut(s)	130, 170, 455
BspANI	GGCC	3 cut(s)	105, 403, 446
BspCNI	CTCAG	2 cut(s)	60, 138
BspFNI	CGCG	1 cut(s)	566
BspHI	TCATGA	1 cut(s)	289
BspLI	GGNNCC	1 cut(s)	402
BspMAI	CTGCAG	1 cut(s)	506
BspMI	ACCTGC	1 cut(s)	325
BspPI	GGATC	1 cut(s)	152
BspQI	GCTCTTC	1 cut(s)	429
BsrDI	GCAATG	1 cut(s)	183
BsrFI	RCCGGY	1 cut(s)	510
BsrI	ACTGG	1 cut(s)	629
BssAI	RCCGGY	1 cut(s)	510
BssMI	GATC	4 cut(s)	144, 247, 292, 598
Bst6I	CTCTTC	1 cut(s)	429
BstC8I	GCNNGC	1 cut(s)	568
BstDEI	CTNAG	3 cut(s)	47, 125, 154
BstF5I	GGATG	1 cut(s)	196
BstFNI	CGCG	1 cut(s)	566
BstH2I	RGCGCY	1 cut(s)	161
BstHHI	GCGC	3 cut(s)	73, 160, 322
BstKTI	GATC	4 cut(s)	147, 250, 295, 601
BstMAI	GTCTC	1 cut(s)	282
BstMBI	GATC	4 cut(s)	144, 247, 292, 598
BstMCI	CGRYCG	1 cut(s)	250
BstMWI	GCNNNNNNNGC	2 cut(s)	443, 477
BstNSI	RCATGY	1 cut(s)	580
BstSCI	CCNGG	1 cut(s)	480
BstSFI	CTRYAG	1 cut(s)	502
BstUI	CGCG	1 cut(s)	566
BstV1I	GCAGC	2 cut(s)	488, 489
BstV2I	GAAGAC	1 cut(s)	553
BstXI	CCANNNNNNTGG	1 cut(s)	526
BsuRI	GGCC	3 cut(s)	105, 403, 446
BtsCI	GGATG	1 cut(s)	196
BtsI	GCAGTG	2 cut(s)	165, 511
BtsIMutI	CAGTG	2 cut(s)	165, 511
BveI	ACCTGC	1 cut(s)	325
Cac8I	GCNNGC	1 cut(s)	568
CciI	TCATGA	1 cut(s)	289
CfoI	GCGC	3 cut(s)	73, 160, 322
Cfr10I	RCCGGY	1 cut(s)	510
Cfr13I	GGNCC	2 cut(s)	401, 445
Csp6I	GTAC	2 cut(s)	466, 516
CspAI	ACCGGT	1 cut(s)	510
CspCI	CAANNNNNGTGG	2 cut(s)	441, 476
CviAII	CATG	4 cut(s)	290, 346, 538, 577
CviQI	GTAC	2 cut(s)	466, 516
DdeI	CTNAG	3 cut(s)	47, 125, 154
DpnI	GATC	4 cut(s)	146, 249, 294, 600
DpnII	GATC	4 cut(s)	144, 247, 292, 598
DraIII	CACNNNGTG	1 cut(s)	349
DriI	GACNNNNNGTC	2 cut(s)	274, 547
Eam1104I	CTCTTC	1 cut(s)	429
Eam1105I	GACNNNNNGTC	2 cut(s)	274, 547
EarI	CTCTTC	1 cut(s)	429
Eco32I	GATATC	1 cut(s)	655
Eco47III	AGCGCT	1 cut(s)	159
EcoO109I	RGGNCCY	1 cut(s)	445
EcoRV	GATATC	1 cut(s)	655
EcoT22I	ATGCAT	1 cut(s)	198
FaeI	CATG	4 cut(s)	293, 349, 541, 580
FaiI	YATR	7 cut(s)	215, 243, 291, 347, 488, 539, 578
FaqI	GGGAC	1 cut(s)	497
FatI	CATG	4 cut(s)	289, 345, 537, 576
FbaI	TGATCA	2 cut(s)	292, 598
Fnu4HI	GCNGC	4 cut(s)	130, 171, 478, 502
FokI	GGATG	1 cut(s)	183
Fsp4HI	GCNGC	4 cut(s)	130, 171, 478, 502
FspBI	CTAG	2 cut(s)	101, 440
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	3 cut(s)	72, 159, 321
GluI	GCNGC	4 cut(s)	130, 171, 478, 502
HaeII	RGCGCY	1 cut(s)	161
HaeIII	GGCC	3 cut(s)	105, 403, 446
HapII	CCGG	2 cut(s)	481, 511
HhaI	GCGC	3 cut(s)	73, 160, 322
Hin1II	CATG	4 cut(s)	293, 349, 541, 580
Hin6I	GCGC	3 cut(s)	71, 158, 320
HinP1I	GCGC	3 cut(s)	71, 158, 320
HinfI	GANTC	5 cut(s)	114, 121, 210, 548, 613
HpaII	CCGG	2 cut(s)	481, 511
HphI	GGTGA	3 cut(s)	272, 400, 608
Hpy166II	GTNNAC	1 cut(s)	516
Hpy188I	TCNGA	2 cut(s)	77, 659
Hpy188III	TCNNGA	4 cut(s)	118, 251, 290, 296
Hpy8I	GTNNAC	1 cut(s)	516
HpyAV	CCTTC	1 cut(s)	254
HpyCH4IV	ACGT	1 cut(s)	335
HpyCH4V	TGCA	7 cut(s)	63, 165, 196, 332, 471, 504, 570
HpyF10VI	GCNNNNNNNGC	2 cut(s)	443, 477
HpyF3I	CTNAG	3 cut(s)	47, 125, 154
HpySE526I	ACGT	1 cut(s)	335
Hsp92II	CATG	4 cut(s)	293, 349, 541, 580
HspAI	GCGC	3 cut(s)	71, 158, 320
Ksp22I	TGATCA	2 cut(s)	292, 598
Kzo9I	GATC	4 cut(s)	144, 247, 292, 598
LguI	GCTCTTC	1 cut(s)	429
LmnI	GCTCC	1 cut(s)	263
LpnPI	CCDG	7 cut(s)	287, 330, 417, 459, 494, 524, 642
Lsp1109I	GCAGC	2 cut(s)	488, 489
LweI	GCATC	2 cut(s)	183, 205
MaeI	CTAG	2 cut(s)	101, 440
MaeII	ACGT	1 cut(s)	335
MaeIII	GTNAC	1 cut(s)	506
MalI	GATC	4 cut(s)	146, 249, 294, 600
MboI	GATC	4 cut(s)	144, 247, 292, 598
MboII	GAAGA	5 cut(s)	14, 24, 89, 416, 553
MfeI	CAATTG	1 cut(s)	589
MhlI	GDGCHC	1 cut(s)	366
MluCI	AATT	2 cut(s)	254, 589
MlyI	GAGTC	1 cut(s)	557
MnlI	CCTC	8 cut(s)	373, 388, 391, 426, 436, 456, 458, 523
Mph1103I	ATGCAT	1 cut(s)	198
MseI	TTAA	2 cut(s)	353, 608
MslI	CAYNNNNRTG	1 cut(s)	288
MspI	CCGG	2 cut(s)	481, 511
MspR9I	CCNGG	1 cut(s)	482
MunI	CAATTG	1 cut(s)	589
MvnI	CGCG	1 cut(s)	566
MwoI	GCNNNNNNNGC	2 cut(s)	443, 477
NciI	CCSGG	1 cut(s)	482
NdeII	GATC	4 cut(s)	144, 247, 292, 598
NlaIII	CATG	4 cut(s)	293, 349, 541, 580
NlaIV	GGNNCC	1 cut(s)	402
NmuCI	GTSAC	1 cut(s)	506
NsbI	TGCGCA	1 cut(s)	321
NsiI	ATGCAT	1 cut(s)	198
NspI	RCATGY	1 cut(s)	580
PagI	TCATGA	1 cut(s)	289
PaqCI	CACCTGC	1 cut(s)	325
PciI	ACATGT	1 cut(s)	576
PciSI	GCTCTTC	1 cut(s)	429
PfeI	GAWTC	4 cut(s)	114, 121, 210, 613
PinAI	ACCGGT	1 cut(s)	510
PkrI	GCNGC	4 cut(s)	131, 172, 479, 503
Ple19I	CGATCG	1 cut(s)	250
PleI	GAGTC	1 cut(s)	556
PpsI	GAGTC	1 cut(s)	556
PscI	ACATGT	1 cut(s)	576
Psp1406I	AACGTT	1 cut(s)	335
PspN4I	GGNNCC	1 cut(s)	402
PspPI	GGNCC	2 cut(s)	401, 445
PstI	CTGCAG	1 cut(s)	506
PvuI	CGATCG	1 cut(s)	250
RsaI	GTAC	2 cut(s)	467, 517
RsaNI	GTAC	2 cut(s)	466, 516
RseI	CAYNNNNRTG	1 cut(s)	288
SapI	GCTCTTC	1 cut(s)	429
SaqAI	TTAA	2 cut(s)	353, 608
SatI	GCNGC	4 cut(s)	130, 171, 478, 502
Sau3AI	GATC	4 cut(s)	144, 247, 292, 598
Sau96I	GGNCC	2 cut(s)	401, 445
SchI	GAGTC	1 cut(s)	557
ScrFI	CCNGG	1 cut(s)	482
SduI	GDGCHC	1 cut(s)	366
SetI	ASST	7 cut(s)	88, 276, 319, 338, 424, 467, 621
SfaNI	GCATC	2 cut(s)	183, 205
SfcI	CTRYAG	1 cut(s)	502
SmiMI	CAYNNNNRTG	1 cut(s)	288
SmlI	CTYRAG	1 cut(s)	417
SmoI	CTYRAG	1 cut(s)	417
Sse9I	AATT	2 cut(s)	254, 589
SsiI	CCGC	3 cut(s)	130, 170, 455
SspI	AATATT	1 cut(s)	19
SspMI	CTAG	2 cut(s)	101, 440
StyD4I	CCNGG	1 cut(s)	480
TaiI	ACGT	1 cut(s)	338
TaqI	TCGA	3 cut(s)	366, 494, 637
TasI	AATT	2 cut(s)	254, 589
TauI	GCSGC	2 cut(s)	132, 173
TfiI	GAWTC	4 cut(s)	114, 121, 210, 613
Tru1I	TTAA	2 cut(s)	353, 608
Tru9I	TTAA	2 cut(s)	353, 608
TscAI	CASTG	2 cut(s)	172, 511
TseFI	GTSAC	1 cut(s)	506
TseI	GCWGC	2 cut(s)	477, 501
Tsp45I	GTSAC	1 cut(s)	506
TspDTI	ATGAA	1 cut(s)	572
TspRI	CASTG	2 cut(s)	172, 511
XapI	RAATTY	1 cut(s)	254
XceI	RCATGY	1 cut(s)	580
XspI	CTAG	2 cut(s)	101, 440
Zsp2I	ATGCAT	1 cut(s)	198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.