Rh5DG242900

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016289
Physical Location & Seq
Forward (+)
27756937 .. 27757912
976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG242900.1

Sequence Viewer

Length: 726 bp
ATGATCATGAGCAGCATGATCTTTAACCCAAGTGCTCTTTTCCATCAATCTCCTGCTCACCCCTGCACTCTTTTATCTTTCATTTTCGCCCTTGGAATCGTTGCCATCTTGTTTTTACTAAGCCAGGACAAGTTTACACCTTCAGGCCGTCTCGCAATCTGGATAGCACTTGCCAAATTAACTCTCTGCACCGAAATTCTATTTCTCATCGCGCTCATGCATCACATTATTGGAGTCATATGTTCTTGGCTTCCATATGGACGCCGTAATGACCGAGATATTGAATCAGCCGGGGTAAGAAGCCACATTCTAACTGGAATTGTTCATTTTGAGCCATCTGATTTCCATCACGATCCCGATGAAGAAACATGGTCGCAAGCACCGGTGCAAAATGTTGTAACTATGTTGCAGATGTTCTTGATGGAAGTGCTTGACAGAGTTTGGGAGGACATGGTAATAGAGGAAGGGCAAGAGCAAAGCTTCCAGGCCATTCCAACCCAAGCCATTGAGGCACTTCCGGGGCCTCTGCAATACTGCAAAGCTAGCCTGGAAATATCGAGCTGCTGCAGCGATCGGTGTGCCATTTGCTTAGAAGATTTCGTGGATGGCGAGTCTTGTCGCGTTTTCGAGTGCAAGCACATCTTTCATGCTAATTGCATTGATAATTGGTTAAAGAATGGTCTAACGTGCCCGATTTGTAGAAACTCTCTTGTGAAGATAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

241

Amino Acids

27.18

Weight (kDa)

5.27

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 192 - 234 2.5e-14 Ring finger domain
zf-C3HC4_2 PF13923 192 - 233 2.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 192 - 234 6.8e-06 zinc-RING finger domain
zf-RING_11 PF17123 193 - 220 4.2e-09 RING-like zinc finger
zf-C3HC4 PF00097 193 - 233 7.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 193 - 234 4.4e-07 RING-H2 zinc finger domain
zf-RING_UBOX PF13445 193 - 231 1.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016289)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g20220
malus_domestica MD03G1226900.v1.1 MD11G1244100.v1.1
prunus_persica Prupe.4G182600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0034041
rosa_laevigata RLG00000033519
rosa_multiflora Rmu_co8356837.1_g000001
rosa_roxburghii Rroxscaffold_1G00046200
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235300 Rh5BG236000 Rh5CG265900 Rh5DG242900
rosa_wichuraiana Rw5G021240 Rw5G021430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 212, 621
AclWI GGATC 1 cut(s) 347
AcsI RAATTY 1 cut(s) 195
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 262
AgeI ACCGGT 1 cut(s) 382
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 3 cut(s) 123, 483, 546
AluBI AGCT 3 cut(s) 480, 542, 561
AluI AGCT 3 cut(s) 480, 542, 561
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 1 cut(s) 347
AoxI GGCC 3 cut(s) 145, 486, 521
ApeKI GCWGC 4 cut(s) 12, 561, 564, 567
ApoI RAATTY 1 cut(s) 195
AsiGI ACCGGT 1 cut(s) 382
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 521
AsuC2I CCSGG 2 cut(s) 292, 519
AsuHPI GGTGA 1 cut(s) 50
AsuNHI GCTAGC 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 4 cut(s) 24, 548, 551, 579
BccI CCATC 6 cut(s) 51, 113, 343, 354, 415, 599
BceAI ACGGC 2 cut(s) 132, 249
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 3 cut(s) 125, 485, 548
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 292, 519
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 1 cut(s) 543
BfmI CTRYAG 1 cut(s) 565
BglI GCCNNNNNGGC 1 cut(s) 509
BisI GCNGC 4 cut(s) 13, 562, 565, 568
BlsI GCNGC 4 cut(s) 14, 563, 566, 569
Bme1390I CCNGG 5 cut(s) 125, 292, 485, 519, 548
BmgT120I GGNCC 1 cut(s) 521
BmiI GGNNCC 1 cut(s) 522
BmrFI CCNGG 5 cut(s) 125, 292, 485, 519, 548
BmsI GCATC 1 cut(s) 229
BmtI GCTAGC 1 cut(s) 546
BpuMI CCSGG 2 cut(s) 292, 519
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 262
BsaJI CCNNGG 3 cut(s) 91, 291, 518
BsaWI WCCGGW 1 cut(s) 382
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 1 cut(s) 319
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 3 cut(s) 125, 485, 548
BseDI CCNNGG 3 cut(s) 91, 291, 518
BseGI GGATG 1 cut(s) 610
BseJI GATNNNNATC 1 cut(s) 345
BseNI ACTGG 1 cut(s) 319
BseSI GKGCMC 1 cut(s) 692
BseXI GCAGC 4 cut(s) 24, 548, 551, 579
BsgI GTGCAG 2 cut(s) 49, 172
Bsh1236I CGCG 2 cut(s) 212, 621
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 3 cut(s) 147, 488, 523
BshTI ACCGGT 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 3 cut(s) 291, 383, 518
BsmAI GTCTC 1 cut(s) 155
BsmBI CGTCTC 1 cut(s) 155
BsnI GGCC 3 cut(s) 147, 488, 523
Bsp1286I GDGCHC 2 cut(s) 37, 692
Bsp143I GATC 4 cut(s) 3, 18, 352, 571
BspANI GGCC 3 cut(s) 147, 488, 523
BspFNI CGCG 2 cut(s) 212, 621
BspHI TCATGA 1 cut(s) 6
BspLI GGNNCC 1 cut(s) 522
BspMAI CTGCAG 1 cut(s) 569
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 1 cut(s) 319
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 91, 291, 518
BssMI GATC 4 cut(s) 3, 18, 352, 571
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 3 cut(s) 125, 485, 548
BstACI GRCGYC 1 cut(s) 262
BstC8I GCNNGC 3 cut(s) 378, 544, 635
BstDEI CTNAG 2 cut(s) 119, 589
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 2 cut(s) 212, 621
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 4 cut(s) 6, 21, 355, 574
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 4 cut(s) 3, 18, 352, 571
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 3 cut(s) 509, 543, 567
BstNI CCWGG 3 cut(s) 125, 485, 548
BstSCI CCNGG 5 cut(s) 123, 290, 483, 517, 546
BstSFI CTRYAG 1 cut(s) 565
BstSLI GKGCMC 1 cut(s) 692
BstUI CGCG 2 cut(s) 212, 621
BstV1I GCAGC 4 cut(s) 24, 548, 551, 579
BsuRI GGCC 3 cut(s) 147, 488, 523
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 610
Cac8I GCNNGC 3 cut(s) 378, 544, 635
CciI TCATGA 1 cut(s) 6
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 521
CseI GACGC 1 cut(s) 270
CspAI ACCGGT 1 cut(s) 382
CviAII CATG 6 cut(s) 7, 16, 217, 369, 451, 647
DdeI CTNAG 2 cut(s) 119, 589
DpnI GATC 4 cut(s) 5, 20, 354, 573
DpnII GATC 4 cut(s) 3, 18, 352, 571
Eco130I CCWWGG 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 521
EcoRII CCWGG 3 cut(s) 123, 483, 546
EcoT14I CCWWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 91
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 6 cut(s) 10, 19, 220, 372, 454, 650
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FatI CATG 6 cut(s) 6, 15, 216, 368, 450, 646
FauNDI CATATG 2 cut(s) 239, 256
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 4 cut(s) 13, 562, 565, 568
FokI GGATG 1 cut(s) 617
Fsp4HI GCNGC 4 cut(s) 13, 562, 565, 568
FspBI CTAG 1 cut(s) 543
GlaI GCGC 1 cut(s) 213
GluI GCNGC 4 cut(s) 13, 562, 565, 568
HaeIII GGCC 3 cut(s) 147, 488, 523
HapII CCGG 3 cut(s) 291, 383, 518
HgaI GACGC 1 cut(s) 270
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 6 cut(s) 10, 19, 220, 372, 454, 650
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HindIII AAGCTT 1 cut(s) 478
HinfI GANTC 4 cut(s) 96, 234, 284, 611
HpaII CCGG 3 cut(s) 291, 383, 518
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 135
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 5 cut(s) 7, 160, 350, 356, 418
Hpy8I GTNNAC 1 cut(s) 135
HpyAV CCTTC 2 cut(s) 150, 458
HpyCH4IV ACGT 1 cut(s) 686
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 543, 567
HpyF3I CTNAG 2 cut(s) 119, 589
HpySE526I ACGT 1 cut(s) 686
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 6 cut(s) 10, 19, 220, 372, 454, 650
HspAI GCGC 1 cut(s) 212
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 18, 352, 571
Lsp1109I GCAGC 4 cut(s) 24, 548, 551, 579
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 686
MaeIII GTNAC 1 cut(s) 397
MalI GATC 4 cut(s) 5, 20, 354, 573
MboI GATC 4 cut(s) 3, 18, 352, 571
MboII GAAGA 2 cut(s) 374, 605
MhlI GDGCHC 2 cut(s) 37, 692
MluCI AATT 5 cut(s) 176, 195, 318, 652, 664
MlyI GAGTC 2 cut(s) 243, 620
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 4 cut(s) 439, 454, 502, 534
Mph1103I ATGCAT 1 cut(s) 222
MseI TTAA 3 cut(s) 24, 179, 671
MspI CCGG 3 cut(s) 291, 383, 518
MspR9I CCNGG 5 cut(s) 125, 292, 485, 519, 548
MvaI CCWGG 3 cut(s) 125, 485, 548
MvnI CGCG 2 cut(s) 212, 621
MwoI GCNNNNNNNGC 3 cut(s) 509, 543, 567
NciI CCSGG 2 cut(s) 292, 519
NdeI CATATG 2 cut(s) 239, 256
NdeII GATC 4 cut(s) 3, 18, 352, 571
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 6 cut(s) 10, 19, 220, 372, 454, 650
NlaIV GGNNCC 1 cut(s) 522
NsiI ATGCAT 1 cut(s) 222
PagI TCATGA 1 cut(s) 6
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PfeI GAWTC 2 cut(s) 96, 284
PinAI ACCGGT 1 cut(s) 382
PkrI GCNGC 4 cut(s) 14, 563, 566, 569
Ple19I CGATCG 1 cut(s) 574
PleI GAGTC 2 cut(s) 242, 619
PpsI GAGTC 2 cut(s) 242, 619
Psp6I CCWGG 3 cut(s) 123, 483, 546
PspGI CCWGG 3 cut(s) 123, 483, 546
PspN4I GGNNCC 1 cut(s) 522
PspPI GGNCC 1 cut(s) 521
PstI CTGCAG 1 cut(s) 569
PvuI CGATCG 1 cut(s) 574
SaqAI TTAA 3 cut(s) 24, 179, 671
SatI GCNGC 4 cut(s) 13, 562, 565, 568
Sau3AI GATC 4 cut(s) 3, 18, 352, 571
Sau96I GGNCC 1 cut(s) 521
SchI GAGTC 2 cut(s) 243, 620
ScrFI CCNGG 5 cut(s) 125, 292, 485, 519, 548
SduI GDGCHC 2 cut(s) 37, 692
SetI ASST 5 cut(s) 142, 482, 544, 563, 689
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 1 cut(s) 565
SgrAI CRCCGGYG 1 cut(s) 382
Sse9I AATT 5 cut(s) 176, 195, 318, 652, 664
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 5 cut(s) 123, 290, 483, 517, 546
StyI CCWWGG 1 cut(s) 91
TaiI ACGT 1 cut(s) 689
TaqI TCGA 2 cut(s) 557, 627
TaqII GACCGA 1 cut(s) 288
TasI AATT 5 cut(s) 176, 195, 318, 652, 664
TfiI GAWTC 2 cut(s) 96, 284
Tru1I TTAA 3 cut(s) 24, 179, 671
Tru9I TTAA 3 cut(s) 24, 179, 671
TseI GCWGC 4 cut(s) 12, 561, 564, 567
TspDTI ATGAA 4 cut(s) 70, 314, 375, 635
XapI RAATTY 1 cut(s) 195
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XspI CTAG 1 cut(s) 543
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.