Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0016289

Physical Location & Seq

Genomic Coordinates Reverse (-)

35506353 .. 35507021

Gene Structure 669 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1244100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGAACACCATGATTTCTAATCCCAGAATTCTTCATCAATCTCCTCATAATCCTCTTAGTTTTTTATCGTGCATTTTTGCATTCGGAATTGCAGGTTTCTTGTTTCTTCTAGGCCAAGCAAAATTAACGACACTCAGCCGCCTTGTGGTTTGGATCCGCAACTTAGTGCTCTGCACCGCGGCTTTGTTTCTCATTGCCTTGATGCATCACATTGTTGGAACCATACTTTCTTGGCTTCCAAACAGAAGCCATAGCGATCAGGAATTTGAAGGTGGAGACGACCGGTCTCATCATCCCCGTGATCAAGACACATTGCCACAAGCACCTGCACAAGATATCGTAACATTGTTCCACTTGGTTTTAATGGAAGTGCTAGATAGAGTTTGGGAGGACTTGGTGATGGAGGAAGGGCAAGAACAAAGGCCTCAGGGTCTCCAAGTTCAAGCCCTAGAGGCCCTCCCACCACCTTTGACGTACTGCAAAGGTGGCCGGGACATAACATATAGTTGCTGCAGTGACCGGTGTACCATTTGCTTGGAGGATTTCGGGGACGGCGAGTTCTGTCGTGTTTTCGCATGCAAACACATGTTTCATTCCGACTGTATTGATCATTGGTTAAAGAACCATCTAACTTGCCCAATTTGTCGAAATTCTGTTGTAGATATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

223

Amino Acids

25.17

Weight (kDa)

5.66

Isoelectric Point (pI)

48.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	173 - 216	1.7e-06	RING-H2 zinc finger domain
zf-RING_2	PF13639	174 - 216	1.2e-13	Ring finger domain
zf-C3HC4_2	PF13923	174 - 215	1.3e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	175 - 202	4.2e-09	RING-like zinc finger
zf-C3HC4	PF00097	175 - 215	1.9e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	175 - 213	7.4e-06	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0016289)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g20220
malus_domestica	MD03G1226900.v1.1 MD11G1244100.v1.1
prunus_persica	Prupe.4G182600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0034041
rosa_laevigata	RLG00000033519
rosa_multiflora	Rmu_co8356837.1_g000001
rosa_roxburghii	Rroxscaffold_1G00046200
rosa_rugosa	Rorug05G0144300
rosa_samantha	Rh5AG235300 Rh5BG236000 Rh5CG265900 Rh5DG242900
rosa_wichuraiana	Rw5G021240 Rw5G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	334
Acc36I	ACCTGC	2 cut(s)	83, 334
AccII	CGCG	1 cut(s)	179
AciI	CCGC	4 cut(s)	139, 157, 177, 179
AclWI	GGATC	2 cut(s)	148, 161
AcoI	YGGCCR	1 cut(s)	487
AcsI	RAATTY	3 cut(s)	27, 263, 649
AfaI	GTAC	2 cut(s)	476, 526
AfiI	CCNNNNNNNGG	1 cut(s)	145
AflIII	ACRYGT	1 cut(s)	585
AgeI	ACCGGT	2 cut(s)	282, 519
AgsI	TTSAA	2 cut(s)	269, 443
AhdI	GACNNNNNGTC	1 cut(s)	283
AloI	GAACNNNNNNTCC	2 cut(s)	542, 574
Alw21I	GWGCWC	1 cut(s)	171
Alw26I	GTCTC	3 cut(s)	270, 291, 437
AlwI	GGATC	2 cut(s)	148, 161
AoxI	GGCC	4 cut(s)	112, 422, 453, 487
ApeKI	GCWGC	1 cut(s)	510
ApoI	RAATTY	3 cut(s)	27, 263, 649
AsiGI	ACCGGT	2 cut(s)	282, 519
AspS9I	GGNCC	1 cut(s)	454
AsuC2I	CCSGG	1 cut(s)	491
AsuHPI	GGTGA	1 cut(s)	409
AxyI	CCTNAGG	1 cut(s)	426
BaeI	ACNNNNGTAYC	2 cut(s)	508, 541
BamHI	GGATCC	1 cut(s)	153
Bbv12I	GWGCWC	1 cut(s)	171
BbvI	GCAGC	1 cut(s)	497
BccI	CCATC	2 cut(s)	394, 633
BceAI	ACGGC	1 cut(s)	568
BclI	TGATCA	2 cut(s)	301, 607
BcnI	CCSGG	1 cut(s)	491
BcoDI	GTCTC	3 cut(s)	270, 291, 437
BfaI	CTAG	3 cut(s)	110, 374, 449
BfmI	CTRYAG	1 cut(s)	511
BfuAI	ACCTGC	2 cut(s)	83, 334
BglI	GCCNNNNNGGC	1 cut(s)	452
BisI	GCNGC	3 cut(s)	139, 180, 511
BlsI	GCNGC	3 cut(s)	140, 181, 512
Bme1390I	CCNGG	1 cut(s)	491
BmeRI	GACNNNNNGTC	1 cut(s)	283
BmgT120I	GGNCC	1 cut(s)	454
BmiI	GGNNCC	2 cut(s)	155, 220
BmrFI	CCNGG	1 cut(s)	491
BmsI	GCATC	2 cut(s)	192, 214
BpuMI	CCSGG	1 cut(s)	491
BsaI	GGTCTC	2 cut(s)	291, 437
BsaJI	CCNNGG	1 cut(s)	177
BsaWI	WCCGGW	2 cut(s)	282, 519
Bsc4I	CCNNNNNNNGG	1 cut(s)	145
Bse118I	RCCGGY	2 cut(s)	282, 519
Bse21I	CCTNAGG	1 cut(s)	426
Bse3DI	GCAATG	2 cut(s)	192, 311
BseDI	CCNNGG	1 cut(s)	177
BseGI	GGATG	1 cut(s)	292
BseLI	CCNNNNNNNGG	1 cut(s)	145
BseMI	GCAATG	2 cut(s)	192, 311
BseMII	CTCAG	2 cut(s)	148, 440
BseRI	GAGGAG	1 cut(s)	33
BseXI	GCAGC	1 cut(s)	497
BsgI	GTGCAG	2 cut(s)	157, 312
Bsh1236I	CGCG	1 cut(s)	179
Bsh1285I	CGRYCG	1 cut(s)	283
BshFI	GGCC	4 cut(s)	114, 424, 455, 489
BshTI	ACCGGT	2 cut(s)	282, 519
BsiEI	CGRYCG	1 cut(s)	283
BsiHKAI	GWGCWC	1 cut(s)	171
BsiSI	CCGG	3 cut(s)	283, 490, 520
BslFI	GGGAC	2 cut(s)	506, 563
BslI	CCNNNNNNNGG	1 cut(s)	145
BsmAI	GTCTC	3 cut(s)	270, 291, 437
BsmBI	CGTCTC	1 cut(s)	270
BsmFI	GGGAC	2 cut(s)	506, 563
BsmI	GAATGC	1 cut(s)	80
BsnI	GGCC	4 cut(s)	114, 424, 455, 489
Bso31I	GGTCTC	2 cut(s)	291, 437
Bsp1286I	GDGCHC	1 cut(s)	171
Bsp143I	GATC	4 cut(s)	153, 256, 301, 607
BspACI	CCGC	4 cut(s)	139, 157, 177, 179
BspANI	GGCC	4 cut(s)	114, 424, 455, 489
BspCNI	CTCAG	2 cut(s)	147, 439
BspFNI	CGCG	1 cut(s)	179
BspLI	GGNNCC	2 cut(s)	155, 220
BspMAI	CTGCAG	1 cut(s)	515
BspMI	ACCTGC	2 cut(s)	83, 334
BspPI	GGATC	2 cut(s)	148, 161
BspTNI	GGTCTC	2 cut(s)	291, 437
BsrDI	GCAATG	2 cut(s)	192, 311
BsrFI	RCCGGY	2 cut(s)	282, 519
BssAI	RCCGGY	2 cut(s)	282, 519
BssECI	CCNNGG	1 cut(s)	177
BssMI	GATC	4 cut(s)	153, 256, 301, 607
Bst4CI	ACNGT	1 cut(s)	602
BstC8I	GCNNGC	1 cut(s)	577
BstDEI	CTNAG	4 cut(s)	56, 134, 163, 426
BstDSI	CCRYGG	1 cut(s)	177
BstF5I	GGATG	1 cut(s)	292
BstFNI	CGCG	1 cut(s)	179
BstKTI	GATC	4 cut(s)	156, 259, 304, 610
BstMAI	GTCTC	3 cut(s)	270, 291, 437
BstMBI	GATC	4 cut(s)	153, 256, 301, 607
BstMCI	CGRYCG	1 cut(s)	283
BstMWI	GCNNNNNNNGC	2 cut(s)	452, 486
BstNSI	RCATGY	2 cut(s)	579, 589
BstSCI	CCNGG	1 cut(s)	489
BstSFI	CTRYAG	1 cut(s)	511
BstUI	CGCG	1 cut(s)	179
BstV1I	GCAGC	1 cut(s)	497
BstX2I	RGATCY	1 cut(s)	153
BstXI	CCANNNNNNTGG	1 cut(s)	535
BstYI	RGATCY	1 cut(s)	153
Bsu36I	CCTNAGG	1 cut(s)	426
BsuRI	GGCC	4 cut(s)	114, 424, 455, 489
BtgI	CCRYGG	1 cut(s)	177
BtsCI	GGATG	1 cut(s)	292
BtsI	GCAGTG	1 cut(s)	520
BtsIMutI	CAGTG	1 cut(s)	520
BveI	ACCTGC	2 cut(s)	83, 334
Cac8I	GCNNGC	1 cut(s)	577
Cfr10I	RCCGGY	2 cut(s)	282, 519
Cfr13I	GGNCC	1 cut(s)	454
Cfr42I	CCGCGG	1 cut(s)	180
Csp6I	GTAC	2 cut(s)	475, 525
CspAI	ACCGGT	2 cut(s)	282, 519
CspCI	CAANNNNNGTGG	2 cut(s)	450, 485
CviAII	CATG	3 cut(s)	10, 576, 586
CviJI	RGCY	9 cut(s)	114, 138, 182, 235, 249, 424, 446, 455, 489
CviKI_1	RGCY	9 cut(s)	114, 138, 182, 235, 249, 424, 446, 455, 489
CviQI	GTAC	2 cut(s)	475, 525
DdeI	CTNAG	4 cut(s)	56, 134, 163, 426
DpnI	GATC	4 cut(s)	155, 258, 303, 609
DpnII	GATC	4 cut(s)	153, 256, 301, 607
DriI	GACNNNNNGTC	1 cut(s)	283
EaeI	YGGCCR	1 cut(s)	487
Eam1105I	GACNNNNNGTC	1 cut(s)	283
Eco147I	AGGCCT	1 cut(s)	424
Eco31I	GGTCTC	2 cut(s)	291, 437
Eco32I	GATATC	2 cut(s)	337, 664
Eco81I	CCTNAGG	1 cut(s)	426
EcoO109I	RGGNCCY	1 cut(s)	454
EcoRI	GAATTC	1 cut(s)	27
EcoRV	GATATC	2 cut(s)	337, 664
EcoT22I	ATGCAT	1 cut(s)	207
Esp3I	CGTCTC	1 cut(s)	270
FaeI	CATG	3 cut(s)	13, 579, 589
FaiI	YATR	9 cut(s)	11, 48, 224, 252, 497, 502, 504, 577, 587
FaqI	GGGAC	2 cut(s)	506, 563
FatI	CATG	3 cut(s)	9, 575, 585
FbaI	TGATCA	2 cut(s)	301, 607
Fnu4HI	GCNGC	3 cut(s)	139, 180, 511
FokI	GGATG	1 cut(s)	279
Fsp4HI	GCNGC	3 cut(s)	139, 180, 511
FspBI	CTAG	3 cut(s)	110, 374, 449
GluI	GCNGC	3 cut(s)	139, 180, 511
HaeIII	GGCC	4 cut(s)	114, 424, 455, 489
HapII	CCGG	3 cut(s)	283, 490, 520
Hin1II	CATG	3 cut(s)	13, 579, 589
HpaII	CCGG	3 cut(s)	283, 490, 520
HphI	GGTGA	1 cut(s)	409
Hpy166II	GTNNAC	1 cut(s)	525
Hpy188I	TCNGA	3 cut(s)	86, 598, 668
Hpy188III	TCNNGA	2 cut(s)	260, 305
Hpy8I	GTNNAC	1 cut(s)	525
HpyAV	CCTTC	2 cut(s)	263, 401
HpyCH4III	ACNGT	1 cut(s)	602
HpyCH4IV	ACGT	1 cut(s)	473
HpyCH4V	TGCA	9 cut(s)	72, 80, 92, 174, 205, 329, 480, 513, 579
HpyF10VI	GCNNNNNNNGC	2 cut(s)	452, 486
HpyF3I	CTNAG	4 cut(s)	56, 134, 163, 426
HpySE526I	ACGT	1 cut(s)	473
Hsp92II	CATG	3 cut(s)	13, 579, 589
Ksp22I	TGATCA	2 cut(s)	301, 607
KspI	CCGCGG	1 cut(s)	180
Kzo9I	GATC	4 cut(s)	153, 256, 301, 607
LpnPI	CCDG	8 cut(s)	37, 78, 245, 296, 339, 413, 503, 533
Lsp1109I	GCAGC	1 cut(s)	497
LweI	GCATC	2 cut(s)	192, 214
MaeI	CTAG	3 cut(s)	110, 374, 449
MaeII	ACGT	1 cut(s)	473
MaeIII	GTNAC	2 cut(s)	340, 515
MalI	GATC	4 cut(s)	155, 258, 303, 609
MboI	GATC	4 cut(s)	153, 256, 301, 607
MboII	GAAGA	2 cut(s)	23, 98
MflI	RGATCY	1 cut(s)	153
MhlI	GDGCHC	1 cut(s)	171
MluCI	AATT	6 cut(s)	27, 87, 122, 263, 639, 649
MmeI	TCCRAC	2 cut(s)	196, 621
MnlI	CCTC	8 cut(s)	54, 63, 382, 397, 435, 445, 467, 532
Mph1103I	ATGCAT	1 cut(s)	207
MseI	TTAA	3 cut(s)	125, 362, 617
MslI	CAYNNNNRTG	1 cut(s)	297
MspA1I	CMGCKG	1 cut(s)	179
MspI	CCGG	3 cut(s)	283, 490, 520
MspR9I	CCNGG	1 cut(s)	491
Mva1269I	GAATGC	1 cut(s)	80
MvnI	CGCG	1 cut(s)	179
MwoI	GCNNNNNNNGC	2 cut(s)	452, 486
NciI	CCSGG	1 cut(s)	491
NdeII	GATC	4 cut(s)	153, 256, 301, 607
NlaIII	CATG	3 cut(s)	13, 579, 589
NlaIV	GGNNCC	2 cut(s)	155, 220
NmuCI	GTSAC	1 cut(s)	515
NsiI	ATGCAT	1 cut(s)	207
NspI	RCATGY	2 cut(s)	579, 589
PaeI	GCATGC	1 cut(s)	579
PaqCI	CACCTGC	1 cut(s)	334
PceI	AGGCCT	1 cut(s)	424
PciI	ACATGT	1 cut(s)	585
PcsI	WCGNNNNNNNCGW	1 cut(s)	552
PctI	GAATGC	1 cut(s)	80
PinAI	ACCGGT	2 cut(s)	282, 519
PkrI	GCNGC	3 cut(s)	140, 181, 512
PscI	ACATGT	1 cut(s)	585
PspN4I	GGNNCC	2 cut(s)	155, 220
PspPI	GGNCC	1 cut(s)	454
PstI	CTGCAG	1 cut(s)	515
PsuI	RGATCY	1 cut(s)	153
RsaI	GTAC	2 cut(s)	476, 526
RsaNI	GTAC	2 cut(s)	475, 525
RseI	CAYNNNNRTG	1 cut(s)	297
SacII	CCGCGG	1 cut(s)	180
SaqAI	TTAA	3 cut(s)	125, 362, 617
SatI	GCNGC	3 cut(s)	139, 180, 511
Sau3AI	GATC	4 cut(s)	153, 256, 301, 607
Sau96I	GGNCC	1 cut(s)	454
ScrFI	CCNGG	1 cut(s)	491
SduI	GDGCHC	1 cut(s)	171
SetI	ASST	6 cut(s)	97, 274, 328, 469, 476, 487
SfaNI	GCATC	2 cut(s)	192, 214
SfcI	CTRYAG	1 cut(s)	511
Sfr303I	CCGCGG	1 cut(s)	180
SgrBI	CCGCGG	1 cut(s)	180
SmiMI	CAYNNNNRTG	1 cut(s)	297
SphI	GCATGC	1 cut(s)	579
Sse9I	AATT	6 cut(s)	27, 87, 122, 263, 639, 649
SseBI	AGGCCT	1 cut(s)	424
SsiI	CCGC	4 cut(s)	139, 157, 177, 179
SspMI	CTAG	3 cut(s)	110, 374, 449
StuI	AGGCCT	1 cut(s)	424
StyD4I	CCNGG	1 cut(s)	489
TaaI	ACNGT	1 cut(s)	602
TaiI	ACGT	1 cut(s)	476
TaqI	TCGA	1 cut(s)	646
TasI	AATT	6 cut(s)	27, 87, 122, 263, 639, 649
TauI	GCSGC	2 cut(s)	141, 182
Tru1I	TTAA	3 cut(s)	125, 362, 617
Tru9I	TTAA	3 cut(s)	125, 362, 617
TscAI	CASTG	1 cut(s)	520
TseFI	GTSAC	1 cut(s)	515
TseI	GCWGC	1 cut(s)	510
Tsp45I	GTSAC	1 cut(s)	515
TspDTI	ATGAA	3 cut(s)	17, 23, 581
TspRI	CASTG	1 cut(s)	520
XapI	RAATTY	3 cut(s)	27, 263, 649
XceI	RCATGY	2 cut(s)	579, 589
XspI	CTAG	3 cut(s)	110, 374, 449
Zsp2I	ATGCAT	1 cut(s)	207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.