Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009809

Physical Location & Seq

Genomic Coordinates Reverse (-)

33844890 .. 33847964

Gene Structure 3075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1250400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGGGTTTCCCCGACATGGGTTCATATACCCCCAAGAACATCCTCATAACTGGGGCTGCTGGCTTTATTGCATCTCATGTTGCCAACCGGCTCATCCGCAGTTACCCTGACTACAACATTGTTGTGCTTGACAAGCTTGACTATTGTTCAAATCTGAAAAACCTCTTTCCCTCGAAGTCATCACCCAATTTCAAGTTTGTTAAGGGGGACATTGGCAGTGCTGACCTTGTCAACTATCTCCTCATCACTGAGTCGATTGATACCATAATGCATTTTGCTGCCCAGACCCATGTAGACAACTCCTTTGGTAACAGCTTTGAGTTCACAAAGAACAACATTTACGGCACCCATGTTCTCCTAGAAGCTTGCAAAGTCACTGGACAAATCAAGAGGTTCATCCATGTCAGCACAGATGAGGTCTATGGTGAGACTGATGAAGATGCTGTTGTGGGAAACCATGAGGCATCTCAACTTCTCCCAACAAATCCATACTCCGCAACAAAAGCGGGAGCAGAAATGCTTGTTATGGCATATGGCAGATCCTATGGATTGCCTGTTATAACGACCCGTGGAAACAATGTCTATGGACCAAATCAATTTCCTGAAAAATTGATTCCGAAGTTTATTCTGTTGGCCATGCAAGGGAAGCCTCTGCCCATTCACGGGGATGGTTCTAACGTGAGAAGCTATCTGTATTGCGAGGATGTTGCTGAGGCCTTTGAGCTCATTCTTCACAAAGGAGAGATTGGACATGTGTACAATATTGGAACAAAGAGGGAAAGGAGAGTTATTGATGTGGCCAAAGATATATGCAGACTTTTCTCAATTGACCCAGAGACAAACATCAAGTTTGTTGAAAACAGACCTTTCAATGATCAGAGGTATTTCCTAGATGATGAGAAGCTGAAGATTTTGGGGTGGTCAGAACGAACTCAGTGGCAAGATGGGTTGAAGAAGACTATAGAATGGTACACTCAGAATCCTGATTGGTGGGGTGATGTATCTGGGGCTCTGCTACCTCATCCTCGAATGCTGATGATGCCTGGTGGGATTGAGAGACAATCGGAAGAGTCTGAAGAGGGTAAATCTGAATCCTTTGTCTCAAGTAATCCCCGAATGTTGGTTCCACCTTCCAAAGCCAGTGGCACTCCTCTTAAACCTCCCTATAAGTTCTTGATCTATGGTAGGACTGGCTGGATTGGGGGTGTACTTGGGAAGCTATGTGAAAAACAAGGGATTCCTTATGAATATGGAAAAGGGCGTCTGGAGGATCGCTCATCACTATTGGCAGATATTCAGAATGTCAAGCCAACCCATGTGTTCAATGCTGCTGGTGTCACTGGTAGACCTAATGTTGATTGGTGCGAATCTCATAAAGCAGAAACAATTCGAGTCAATGTTGCTGGAACCTTGACCTTGGCAGATGTTTGCAGACAGCATGGACTCCTGATGATGAACTTTGCTACTGGGTGTATATTTGAATATGACGCTAAACATCCAGAGGGTTCGGGAATTGGGTTCAAGGAAGAAGACACACCCAATTTCCACGGTTCTTTCTACTCAAAAACCAAGGCCATGGTTGAGGAACTCTTGAAAGAATTCGACAATGTCTGCACTCTCAGAGTTCGGATGCCCATCTCATCTGACCTGAACAACCCTCGCAACTTCATCACCAAGATCTCTCGTTATAACAAAGTGGTAAACATTCCTAACAGCATGACCATCCTGGATGAATTACTACCCATTTCTATTGAAATGGCGAAGCGGAACTTGAGGGGCATATGGAACTTCACAAACCCTGGCGTCGTGAGCCATAATGAGATCCTGGAGATGTACAAGAAATACATTGACCCGAAGTTTACATGGGCTAACTTCACGCTCGAAGAGCAAGCCAAGGTTATAGTTGCAGCTCGAAGCAACAACGAAATGGATGCGTCCAAGTTGAAGAAAGAGTTCCCCGAGTTGCTACCAATCAAGGAGTCGCTGATTAAGTATGTCTTTGAACCAAACAAGAAGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

674

Amino Acids

75.91

Weight (kDa)

6.62

Isoelectric Point (pI)

34.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	13 - 323	3.1e-14	RmlD substrate binding domain
Epimerase	PF01370	14 - 256	9.6e-56	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	14 - 288	4.1e-20	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	15 - 320	9.4e-66	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	15 - 240	5e-23	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	16 - 196	2.7e-18	Male sterility protein
RmlD_sub_bind	PF04321	390 - 560	1.8e-13	RmlD substrate binding domain
Epimerase	PF01370	394 - 543	8.7e-12	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78570
fragaria_vesca	FvH4_3g17640 FvH4_3g17640 FvH4_3g17640
malus_domestica	MD03G1250400.v1.1 MD11G1270500.v1.1
prunus_persica	Prupe.4G155800_v2.0.a1 Prupe.4G155800_v2.0.a1 Prupe.4G155800_v2.0.a1
pyrus_communis	pycom11g23940
rosa_chinensis	RchiOBHm_Chr5g0029371
rosa_laevigata	RLG00000033159
rosa_multiflora	Rmu_sc0004373.1_g000022
rosa_roxburghii	Rroxscaffold_1G00050590
rosa_rugosa	Rorug05G0113800 Rorug05G0113900
rosa_samantha	Rh5AG205700 Rh5BG204100 Rh5CG226000 Rh5DG207700
rosa_wichuraiana	Rw5G018720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	560, 1689
AccB1I	GGYRCC	1 cut(s)	344
AccI	GTMKAC	2 cut(s)	294, 1345
AciI	CCGC	4 cut(s)	97, 495, 506, 1765
AclWI	GGATC	3 cut(s)	534, 1278, 1816
AcoI	YGGCCR	2 cut(s)	633, 798
AcsI	RAATTY	1 cut(s)	1598
AcuI	CTGAAG	2 cut(s)	926, 1095
AcyI	GRCGYC	2 cut(s)	1261, 1803
AfaI	GTAC	4 cut(s)	758, 971, 1209, 1835
AfiI	CCNNNNNNNGG	6 cut(s)	16, 17, 642, 662, 663, 1120
AflIII	ACRYGT	1 cut(s)	751
AjnI	CCWGG	4 cut(s)	1042, 1725, 1798, 1824
AluBI	AGCT	8 cut(s)	136, 315, 365, 687, 724, 904, 1219, 1910
AluI	AGCT	8 cut(s)	136, 315, 365, 687, 724, 904, 1219, 1910
Alw21I	GWGCWC	1 cut(s)	726
Alw26I	GTCTC	4 cut(s)	422, 830, 1051, 1105
AlwI	GGATC	3 cut(s)	534, 1278, 1816
Ama87I	CYCGRG	1 cut(s)	1958
AoxI	GGCC	4 cut(s)	633, 714, 798, 1572
ApeKI	GCWGC	4 cut(s)	56, 278, 1328, 1907
ApoI	RAATTY	1 cut(s)	1598
ArsI	GACNNNNNNTTYG	2 cut(s)	287, 319
Asp700I	GAANNNNTTC	3 cut(s)	1386, 1598, 1952
AspS9I	GGNCC	1 cut(s)	587
AsuHPI	GGTGA	4 cut(s)	174, 437, 1007, 1663
AvaI	CYCGRG	1 cut(s)	1958
AvaII	GGWCC	1 cut(s)	587
BalI	TGGCCA	2 cut(s)	635, 800
BanI	GGYRCC	1 cut(s)	344
BanII	GRGCYC	2 cut(s)	726, 1012
BbsI	GAAGAC	2 cut(s)	962, 1536
Bbv12I	GWGCWC	1 cut(s)	726
BbvCI	CCTCAGC	1 cut(s)	711
BbvI	GCAGC	4 cut(s)	43, 265, 1315, 1919
BccI	CCATC	4 cut(s)	662, 938, 1643, 1730
BceAI	ACGGC	1 cut(s)	358
BcgI	CGANNNNNNTGC	4 cut(s)	689, 723, 1913, 1947
BciT130I	CCWGG	4 cut(s)	1044, 1727, 1800, 1826
BclI	TGATCA	1 cut(s)	874
BcoDI	GTCTC	4 cut(s)	422, 830, 1051, 1105
BfaI	CTAG	2 cut(s)	359, 890
BfmI	CTRYAG	1 cut(s)	960
BglII	AGATCT	1 cut(s)	1677
BisI	GCNGC	4 cut(s)	57, 279, 1329, 1908
BlsI	GCNGC	4 cut(s)	58, 280, 1330, 1909
Bme1390I	CCNGG	4 cut(s)	1044, 1727, 1800, 1826
Bme18I	GGWCC	1 cut(s)	587
BmeT110I	CYCGRG	1 cut(s)	1958
BmgT120I	GGNCC	1 cut(s)	587
BmiI	GGNNCC	3 cut(s)	346, 1125, 1408
BmrFI	CCNGG	4 cut(s)	1044, 1727, 1800, 1826
BmrI	ACTGGG	2 cut(s)	60, 1476
BmsI	GCATC	6 cut(s)	80, 430, 473, 1029, 1620, 1921
BmuI	ACTGGG	2 cut(s)	60, 1476
BpiI	GAAGAC	2 cut(s)	962, 1536
BplI	GAGNNNNNCTC	2 cut(s)	1259, 1291
BpmI	CTGGAG	2 cut(s)	1286, 1847
Bpu10I	CCTNAGC	1 cut(s)	711
BpuEI	CTTGAG	2 cut(s)	1087, 1792
BsaBI	GATNNNNATC	1 cut(s)	1634
BsaHI	GRCGYC	2 cut(s)	1261, 1803
BsaJI	CCNNGG	7 cut(s)	568, 1416, 1546, 1569, 1575, 1798, 1893
Bsc4I	CCNNNNNNNGG	6 cut(s)	16, 17, 642, 662, 663, 1120
Bse118I	RCCGGY	1 cut(s)	87
Bse1I	ACTGG	6 cut(s)	55, 382, 1140, 1195, 1345, 1471
Bse8I	GATNNNNATC	1 cut(s)	1634
BseBI	CCWGG	4 cut(s)	1044, 1727, 1800, 1826
BseDI	CCNNGG	7 cut(s)	568, 1416, 1546, 1569, 1575, 1798, 1893
BseJI	GATNNNNATC	1 cut(s)	1634
BseLI	CCNNNNNNNGG	6 cut(s)	16, 17, 642, 662, 663, 1120
BseMII	CTCAG	5 cut(s)	240, 702, 947, 989, 1633
BseNI	ACTGG	6 cut(s)	55, 382, 1140, 1195, 1345, 1471
BseRI	GAGGAG	2 cut(s)	230, 1140
BseXI	GCAGC	4 cut(s)	43, 265, 1315, 1919
BsgI	GTGCAG	1 cut(s)	1597
BshFI	GGCC	4 cut(s)	635, 716, 800, 1574
BshNI	GGYRCC	1 cut(s)	344
BsiHKAI	GWGCWC	1 cut(s)	726
BsiHKCI	CYCGRG	1 cut(s)	1958
BsiSI	CCGG	1 cut(s)	88
BslFI	GGGAC	1 cut(s)	221
BslI	CCNNNNNNNGG	6 cut(s)	16, 17, 642, 662, 663, 1120
BsmAI	GTCTC	4 cut(s)	422, 830, 1051, 1105
BsmFI	GGGAC	1 cut(s)	221
BsmI	GAATGC	1 cut(s)	1035
BsnI	GGCC	4 cut(s)	635, 716, 800, 1574
BsoBI	CYCGRG	1 cut(s)	1958
Bsp1286I	GDGCHC	2 cut(s)	726, 1012
Bsp1407I	TGTACA	2 cut(s)	756, 1833
Bsp143I	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
Bsp19I	CCATGG	1 cut(s)	1575
BspACI	CCGC	4 cut(s)	97, 495, 506, 1765
BspANI	GGCC	4 cut(s)	635, 716, 800, 1574
BspCNI	CTCAG	5 cut(s)	241, 703, 946, 988, 1632
BspLI	GGNNCC	3 cut(s)	346, 1125, 1408
BspPI	GGATC	3 cut(s)	534, 1278, 1816
BspQI	GCTCTTC	1 cut(s)	1878
BspT107I	GGYRCC	1 cut(s)	344
BsrFI	RCCGGY	1 cut(s)	87
BsrGI	TGTACA	2 cut(s)	756, 1833
BsrI	ACTGG	6 cut(s)	55, 382, 1140, 1195, 1345, 1471
BssAI	RCCGGY	1 cut(s)	87
BssECI	CCNNGG	7 cut(s)	568, 1416, 1546, 1569, 1575, 1798, 1893
BssMI	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
BssNI	GRCGYC	2 cut(s)	1261, 1803
BssT1I	CCWWGG	4 cut(s)	1416, 1569, 1575, 1893
Bst2UI	CCWGG	4 cut(s)	1044, 1727, 1800, 1826
Bst4CI	ACNGT	1 cut(s)	1550
Bst6I	CTCTTC	3 cut(s)	1062, 1071, 1878
BstACI	GRCGYC	2 cut(s)	1261, 1803
BstAUI	TGTACA	2 cut(s)	756, 1833
BstC8I	GCNNGC	3 cut(s)	61, 367, 1890
BstDEI	CTNAG	5 cut(s)	249, 711, 933, 975, 1619
BstDSI	CCRYGG	3 cut(s)	568, 1546, 1575
BstKTI	GATC	6 cut(s)	542, 877, 1179, 1273, 1680, 1824
BstMAI	GTCTC	4 cut(s)	422, 830, 1051, 1105
BstMBI	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
BstMWI	GCNNNNNNNGC	6 cut(s)	133, 503, 646, 1039, 1809, 1885
BstNI	CCWGG	4 cut(s)	1044, 1727, 1800, 1826
BstNSI	RCATGY	1 cut(s)	755
BstSCI	CCNGG	4 cut(s)	1042, 1725, 1798, 1824
BstSFI	CTRYAG	1 cut(s)	960
BstV1I	GCAGC	4 cut(s)	43, 265, 1315, 1919
BstV2I	GAAGAC	2 cut(s)	962, 1536
BstX2I	RGATCY	3 cut(s)	539, 1677, 1821
BstXI	CCANNNNNNTGG	1 cut(s)	1576
BstYI	RGATCY	3 cut(s)	539, 1677, 1821
BsuRI	GGCC	4 cut(s)	635, 716, 800, 1574
BtgI	CCRYGG	3 cut(s)	568, 1546, 1575
BtsI	GCAGTG	1 cut(s)	223
BtsIMutI	CAGTG	6 cut(s)	223, 246, 375, 941, 1147, 1338
Cac8I	GCNNGC	3 cut(s)	61, 367, 1890
Cfr10I	RCCGGY	1 cut(s)	87
Cfr13I	GGNCC	1 cut(s)	587
CseI	GACGC	4 cut(s)	1250, 1496, 1792, 1923
Csp6I	GTAC	4 cut(s)	757, 970, 1208, 1834
CspCI	CAANNNNNGTGG	2 cut(s)	1123, 1158
CviQI	GTAC	4 cut(s)	757, 970, 1208, 1834
DdeI	CTNAG	5 cut(s)	249, 711, 933, 975, 1619
DpnI	GATC	6 cut(s)	541, 876, 1178, 1272, 1679, 1823
DpnII	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
EaeI	YGGCCR	2 cut(s)	633, 798
Eam1104I	CTCTTC	3 cut(s)	1062, 1071, 1878
EarI	CTCTTC	3 cut(s)	1062, 1071, 1878
Ecl136II	GAGCTC	1 cut(s)	724
Eco130I	CCWWGG	4 cut(s)	1416, 1569, 1575, 1893
Eco147I	AGGCCT	1 cut(s)	716
Eco24I	GRGCYC	2 cut(s)	726, 1012
Eco47I	GGWCC	1 cut(s)	587
Eco53kI	GAGCTC	1 cut(s)	724
Eco57I	CTGAAG	2 cut(s)	926, 1095
Eco88I	CYCGRG	1 cut(s)	1958
EcoICRI	GAGCTC	1 cut(s)	724
EcoRI	GAATTC	1 cut(s)	1598
EcoRII	CCWGG	4 cut(s)	1042, 1725, 1798, 1824
EcoT14I	CCWWGG	4 cut(s)	1416, 1569, 1575, 1893
EcoT22I	ATGCAT	1 cut(s)	273
EcoT38I	GRGCYC	2 cut(s)	726, 1012
ErhI	CCWWGG	4 cut(s)	1416, 1569, 1575, 1893
FalI	AAGNNNNNCTT	4 cut(s)	1754, 1786, 1982, 2014
FaqI	GGGAC	1 cut(s)	221
FauI	CCCGC	1 cut(s)	499
FauNDI	CATATG	2 cut(s)	532, 1781
FbaI	TGATCA	1 cut(s)	874
FblI	GTMKAC	2 cut(s)	294, 1345
Fnu4HI	GCNGC	4 cut(s)	57, 279, 1329, 1908
FriOI	GRGCYC	2 cut(s)	726, 1012
Fsp4HI	GCNGC	4 cut(s)	57, 279, 1329, 1908
FspBI	CTAG	2 cut(s)	359, 890
GluI	GCNGC	4 cut(s)	57, 279, 1329, 1908
GsuI	CTGGAG	2 cut(s)	1286, 1847
HaeIII	GGCC	4 cut(s)	635, 716, 800, 1574
HapII	CCGG	1 cut(s)	88
HgaI	GACGC	4 cut(s)	1250, 1496, 1792, 1923
Hin1I	GRCGYC	2 cut(s)	1261, 1803
HincII	GTYRAC	1 cut(s)	232
HindII	GTYRAC	1 cut(s)	232
HindIII	AAGCTT	2 cut(s)	134, 363
HpaII	CCGG	1 cut(s)	88
HphI	GGTGA	4 cut(s)	174, 437, 1007, 1663
Hpy166II	GTNNAC	9 cut(s)	232, 295, 324, 757, 972, 1208, 1346, 1702, 1860
Hpy8I	GTNNAC	9 cut(s)	232, 295, 324, 757, 972, 1208, 1346, 1702, 1860
Hpy99I	CGWCG	1 cut(s)	1808
HpyAV	CCTTC	2 cut(s)	1140, 2008
HpyCH4III	ACNGT	1 cut(s)	1550
HpyCH4IV	ACGT	1 cut(s)	678
HpyCH4V	TGCA	8 cut(s)	71, 271, 369, 640, 813, 1431, 1614, 1907
HpyF10VI	GCNNNNNNNGC	6 cut(s)	133, 503, 646, 1039, 1809, 1885
HpyF3I	CTNAG	5 cut(s)	249, 711, 933, 975, 1619
HpySE526I	ACGT	1 cut(s)	678
Hsp92I	GRCGYC	2 cut(s)	1261, 1803
Ksp22I	TGATCA	1 cut(s)	874
Kzo9I	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
LguI	GCTCTTC	1 cut(s)	1878
LmnI	GCTCC	1 cut(s)	509
Lsp1109I	GCAGC	4 cut(s)	43, 265, 1315, 1919
LweI	GCATC	6 cut(s)	80, 430, 473, 1029, 1620, 1921
MaeI	CTAG	2 cut(s)	359, 890
MaeII	ACGT	1 cut(s)	678
MaeIII	GTNAC	4 cut(s)	101, 308, 373, 1336
MalI	GATC	6 cut(s)	541, 876, 1178, 1272, 1679, 1823
MboI	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
MfeI	CAATTG	1 cut(s)	825
MflI	RGATCY	3 cut(s)	539, 1677, 1821
MhlI	GDGCHC	2 cut(s)	726, 1012
MlsI	TGGCCA	2 cut(s)	635, 800
MluCI	AATT	9 cut(s)	187, 596, 608, 825, 1386, 1512, 1540, 1598, 1733
MluNI	TGGCCA	2 cut(s)	635, 800
MlyI	GAGTC	5 cut(s)	260, 1079, 1401, 1437, 1988
Mox20I	TGGCCA	2 cut(s)	635, 800
Mph1103I	ATGCAT	1 cut(s)	273
MroXI	GAANNNNTTC	3 cut(s)	1386, 1598, 1952
MscI	TGGCCA	2 cut(s)	635, 800
MseI	TTAA	3 cut(s)	201, 1155, 1989
MslI	CAYNNNNRTG	2 cut(s)	122, 666
Msp20I	TGGCCA	2 cut(s)	635, 800
MspI	CCGG	1 cut(s)	88
MspR9I	CCNGG	4 cut(s)	1044, 1727, 1800, 1826
MunI	CAATTG	1 cut(s)	825
Mva1269I	GAATGC	1 cut(s)	1035
MvaI	CCWGG	4 cut(s)	1044, 1727, 1800, 1826
MwoI	GCNNNNNNNGC	6 cut(s)	133, 503, 646, 1039, 1809, 1885
NcoI	CCATGG	1 cut(s)	1575
NdeI	CATATG	2 cut(s)	532, 1781
NdeII	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
NlaIV	GGNNCC	3 cut(s)	346, 1125, 1408
NmuCI	GTSAC	2 cut(s)	373, 1336
NsiI	ATGCAT	1 cut(s)	273
NspI	RCATGY	1 cut(s)	755
PceI	AGGCCT	1 cut(s)	716
PciI	ACATGT	1 cut(s)	751
PciSI	GCTCTTC	1 cut(s)	1878
PctI	GAATGC	1 cut(s)	1035
PdmI	GAANNNNTTC	3 cut(s)	1386, 1598, 1952
PfeI	GAWTC	5 cut(s)	613, 979, 1091, 1237, 1367
PflFI	GACNNNGTC	2 cut(s)	227, 1607
PfoI	TCCNGGA	2 cut(s)	1725, 1824
PkrI	GCNGC	4 cut(s)	58, 280, 1330, 1909
PleI	GAGTC	5 cut(s)	259, 1078, 1400, 1437, 1987
PpsI	GAGTC	5 cut(s)	259, 1078, 1400, 1437, 1987
PscI	ACATGT	1 cut(s)	751
PsiI	TTATAA	2 cut(s)	560, 1689
Psp124BI	GAGCTC	1 cut(s)	726
Psp6I	CCWGG	4 cut(s)	1042, 1725, 1798, 1824
PspGI	CCWGG	4 cut(s)	1042, 1725, 1798, 1824
PspN4I	GGNNCC	3 cut(s)	346, 1125, 1408
PspPI	GGNCC	1 cut(s)	587
PsrI	GAACNNNNNNTAC	2 cut(s)	323, 355
PsuI	RGATCY	3 cut(s)	539, 1677, 1821
PsyI	GACNNNGTC	2 cut(s)	227, 1607
RsaI	GTAC	4 cut(s)	758, 971, 1209, 1835
RsaNI	GTAC	4 cut(s)	757, 970, 1208, 1834
RseI	CAYNNNNRTG	2 cut(s)	122, 666
SacI	GAGCTC	1 cut(s)	726
SapI	GCTCTTC	1 cut(s)	1878
SaqAI	TTAA	3 cut(s)	201, 1155, 1989
SatI	GCNGC	4 cut(s)	57, 279, 1329, 1908
Sau3AI	GATC	6 cut(s)	539, 874, 1176, 1270, 1677, 1821
Sau96I	GGNCC	1 cut(s)	587
SchI	GAGTC	5 cut(s)	260, 1079, 1401, 1437, 1988
ScrFI	CCNGG	4 cut(s)	1044, 1727, 1800, 1826
SduI	GDGCHC	2 cut(s)	726, 1012
SfaNI	GCATC	6 cut(s)	80, 430, 473, 1029, 1620, 1921
SfcI	CTRYAG	1 cut(s)	960
SinI	GGWCC	1 cut(s)	587
SmiMI	CAYNNNNRTG	2 cut(s)	122, 666
SmlI	CTYRAG	2 cut(s)	1102, 1771
SmoI	CTYRAG	2 cut(s)	1102, 1771
Sse9I	AATT	9 cut(s)	187, 596, 608, 825, 1386, 1512, 1540, 1598, 1733
SseBI	AGGCCT	1 cut(s)	716
SsiI	CCGC	4 cut(s)	97, 495, 506, 1765
SspI	AATATT	1 cut(s)	763
SspMI	CTAG	2 cut(s)	359, 890
SstI	GAGCTC	1 cut(s)	726
StuI	AGGCCT	1 cut(s)	716
StyD4I	CCNGG	4 cut(s)	1042, 1725, 1798, 1824
StyI	CCWWGG	4 cut(s)	1416, 1569, 1575, 1893
TaaI	ACNGT	1 cut(s)	1550
TaiI	ACGT	1 cut(s)	681
TaqI	TCGA	7 cut(s)	173, 254, 1027, 1390, 1602, 1881, 1912
TasI	AATT	9 cut(s)	187, 596, 608, 825, 1386, 1512, 1540, 1598, 1733
TatI	WGTACW	3 cut(s)	756, 1207, 1833
TfiI	GAWTC	5 cut(s)	613, 979, 1091, 1237, 1367
Tru1I	TTAA	3 cut(s)	201, 1155, 1989
Tru9I	TTAA	3 cut(s)	201, 1155, 1989
TscAI	CASTG	6 cut(s)	223, 253, 382, 941, 1147, 1345
TseFI	GTSAC	2 cut(s)	373, 1336
TseI	GCWGC	4 cut(s)	56, 278, 1328, 1907
Tsp45I	GTSAC	2 cut(s)	373, 1336
TspDTI	ATGAA	7 cut(s)	12, 385, 450, 1260, 1469, 1657, 1746
TspRI	CASTG	6 cut(s)	223, 253, 382, 941, 1147, 1345
Tth111I	GACNNNGTC	2 cut(s)	227, 1607
VpaK11BI	GGWCC	1 cut(s)	587
XapI	RAATTY	1 cut(s)	1598
XceI	RCATGY	1 cut(s)	755
XmiI	GTMKAC	2 cut(s)	294, 1345
XmnI	GAANNNNTTC	3 cut(s)	1386, 1598, 1952
XspI	CTAG	2 cut(s)	359, 890
Zsp2I	ATGCAT	1 cut(s)	273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.