Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0009809

Physical Location & Seq

Genomic Coordinates Forward (+)

23870768 .. 23875811

Gene Structure 5044 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033159

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGAAGCCGTGACAGGCTTTGGATCACCAAATTTGACTTATCATCCCAATCTTTGCGCACAACCTACCCGCTACAAACCAGGCCAAAAAGGTGCTCCGCCATTTCGCAATCCCCCCCCCACCACCCCAAGGGCATTTTCGCCCATTCACCCCGCCACCACCTCTGATTGTTTCCGCGATCTACAAAGACGCCCTCTTGAGGCTTTTTTCTTTTTATTTTTGACCCTCAATTCTCTTCTCTGGGCTGGTCTTCGATCTTTGATCCCCAAGTCAAAATTAAGTTGCCTTGACATGGCTTCATATACACCCAAAAACATCCTCATAACTGGGGCAGCTGGCTTCATTGCTTCCCATGTGGCCAACCGGCTCGTTCGCAGTCACCCGGAATACAATATCGTTGTGCTTGACAAGCTTGACTATTGTTCGAATCTGAAAAACCTCCTTCCCTCTAGGTCGTCACCCAATTTCAAGTTTGTTAAGGGGGACATAGGAAGTGCTGACCTTGTCAACTATCTCCTCATCACTGAGTCGATAGATACAATAATGCATTTTGCAGCCCAGACCCATGTCGACAACTCCTTTGGTAACAGCTTTGAGTTCACAAAGAACAACATCTATGGCACCCATGTTCTTCTAGAAGCATGTAAAGTCACTGGCCAAATCAGGAGGTTCATCCATGTTAGCACAGATGAGGTCTATGGTGAGACTGATGAAGATGCTGTTGTAGGAAATCATGAGGCATCTCAACTTCTTCCAACAAATCCATACTCTGCAACAAAAGCTGGAGCAGAAATGCTTGTTATGGCATATGGAAGGTCATATGGGTTGCCTGTTATAACGACCCGCGGAAACAATGTTTATGGACCAAATCAATTTCCTGAAAAATTGATTCCAAAGTTCATTCTCTTAGCTATGCAAGGGAAACCTCTTCCCATTCATGGGGATGGTGCCAATGTGAGGAGCTACTTATACTGTGAGGATGTTGCTGAGGCTTTTGAACTCATTCTTCACAAAGGAGAGGTTGGCCATGTGTACAATATTGGGACAAAGAAGGAAAGGAGAGTTATTGATGTGGCCAGAGATATATGCAGACTTTTCAATGTGGACTCGGAAGCATGCATCAAGTTTGTTGAAAACAGACCTTTCAATGACCAGAGGTATTTTCTTGATGATCAGAAGTTGACGGTGTTGGGGTGGTCAGAGCGGACTACCTGGGAAGAGGGGTTGAAGAAGACTATAGAATGGTACACTAAGAATCCTAACTGGTGGGGTGATGTATCTGGGGCACTGCTTCCTCATCCACGAATGTTGATGATGCCCGGTGGAATGGAGAGACATTTTGATGAACCTGAAGAGGAAAAATCTGAGTCTTATGTCTCAAGTAATCCTCGAATGTTGGTTCCCCCTTCCAAATGCAGTGGCTCTCCTCGTAAACCTCCTTATAAGTTCTTGATCTATGGTAAGACCGGGTGGATTGGTGGTGTACTTGGGAAGCTCTGTGAAAAACAAGGGATTCCTTATGAATATGGAAAAGGGCGTCTTGAGGATCGGCCTTCACTTGCTGCAGATATTCAGAATATTAAGCCAACTCATGTGTTTAATGCTGCTGGTGTGACTGGTAGACCTAATGTTGATTGGTGTGAATCTCATAGAGCAGAAACAATTCGAACCAATGTTGCTGGAACCCTAACCCTAGCAGATGTTTGCAGAGAGCATGGGCTCCTTATGATCAATTTTGCGACTGGGTGTATATTTGAGTATGATGCTAAACATCCGGAGGGTTCAGGGATTGGGTTCACTGAAGAAGACAAACCCAATTTCTTTGGTTCTTTCTATTCAAAAACCAAGGCCATGGTTGAGGAGCTATTGAATGAATTCGACAATGTTTGCACTCTTAGAGTTCGAATGCCCATCTCATCAGACCTGAACAACCCACGTAACTTCATCACCAAGATTTCTCGTTATAACAAAGTGGTTAACATTCCTAACAGCATGACCATCTTGGATGAACTACTACCTATTTCAATCGAGATGGCAAAGCGGAACTTGAGGGGTATCTGGAACTTCACAAATCCTGGGGTCGTGAGCCATAATGAGATTCTGGAAATGTACAAGCAATACATTGACCCAAGTTTCAAATGGGCAAACTTCACAATTGAAGAGCAAGCCAAGGTTATAGTTGCCGCCCGAAGCAACAATGAAATGGATGCATCCAAGTTGAAGAAAGAGTTCCCCGAGTTGCTACCAATCAAGGAGTCGCTGATAAAACACACGCTTGTTCTGGCCTCGACCCGCGAAAAGCCTATAGCAGCTGTGAAATCAAAGCCTGTAGCTACAGGCTTTGATTGCGTTGTTGACCAAAGTATAAGCGATGCCTTCATGCAATTCACCATAGTTTGGACTGCAATTTCTGAAGTCTACAGCTGCAGTTTCGCCGCCATGCACTACAGTCAGTTCTTCGGTGGCTTCAGCTCGGGTGGGACAAGAAGATTTGGTCATGGACTCGAGCATCTAACCAGCAGACTCCCTACCGGCTCAGTCTATTGGTCTTTGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Epimerase GDP_Man_Dehyd

Protein Analysis

853

Amino Acids

95.63

Weight (kDa)

7.81

Isoelectric Point (pI)

37.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	105 - 415	1.3e-14	RmlD substrate binding domain
Epimerase	PF01370	106 - 348	7.8e-55	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	106 - 377	8.5e-20	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	107 - 412	6.3e-65	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	107 - 332	4.5e-23	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	108 - 288	4.3e-18	Male sterility protein
RmlD_sub_bind	PF04321	482 - 652	1.6e-13	RmlD substrate binding domain
Epimerase	PF01370	485 - 634	1.2e-10	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78570
fragaria_vesca	FvH4_3g17640 FvH4_3g17640 FvH4_3g17640
malus_domestica	MD03G1250400.v1.1 MD11G1270500.v1.1
prunus_persica	Prupe.4G155800_v2.0.a1 Prupe.4G155800_v2.0.a1 Prupe.4G155800_v2.0.a1
pyrus_communis	pycom11g23940
rosa_chinensis	RchiOBHm_Chr5g0029371
rosa_laevigata	RLG00000033159
rosa_multiflora	Rmu_sc0004373.1_g000022
rosa_roxburghii	Rroxscaffold_1G00050590
rosa_rugosa	Rorug05G0113800 Rorug05G0113900
rosa_samantha	Rh5AG205700 Rh5BG204100 Rh5CG226000 Rh5DG207700
rosa_wichuraiana	Rw5G018720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	836, 1443, 1965
Acc16I	TGCGCA	1 cut(s)	58
AccB1I	GGYRCC	2 cut(s)	620, 947
AccB7I	CCANNNNNTGG	1 cut(s)	2397
AccBSI	CCGCTC	1 cut(s)	1204
AccI	GTMKAC	3 cut(s)	570, 1621, 2418
AccII	CGCG	3 cut(s)	177, 846, 2295
AccIII	TCCGGA	1 cut(s)	1774
AclWI	GGATC	3 cut(s)	31, 256, 1554
AcoI	YGGCCR	4 cut(s)	357, 655, 1024, 1074
AcsI	RAATTY	2 cut(s)	31, 1874
AcuI	CTGAAG	4 cut(s)	1371, 1821, 2433, 2452
AcyI	GRCGYC	2 cut(s)	190, 1537
AfaI	GTAC	4 cut(s)	1034, 1247, 1485, 2111
AfiI	CCNNNNNNNGG	7 cut(s)	129, 199, 292, 938, 939, 957, 2397
AjnI	CCWGG	3 cut(s)	79, 1211, 2074
AjuI	GAANNNNNNNTTGG	2 cut(s)	121, 153
Alw21I	GWGCWC	1 cut(s)	97
Alw26I	GTCTC	3 cut(s)	698, 1327, 1381
AlwI	GGATC	3 cut(s)	31, 256, 1554
Ama87I	CYCGRG	3 cut(s)	2234, 2473, 2504
Aor13HI	TCCGGA	1 cut(s)	1774
AoxI	GGCC	8 cut(s)	82, 357, 655, 1024, 1074, 1550, 1848, 2283
ApeKI	GCWGC	6 cut(s)	332, 554, 1562, 1604, 2309, 2424
ApoI	RAATTY	2 cut(s)	31, 1874
ArsI	GACNNNNNNTTYG	2 cut(s)	563, 595
Asp700I	GAANNNNTTC	4 cut(s)	1002, 1662, 1874, 2228
AspLEI	GCGC	1 cut(s)	59
AspS9I	GGNCC	1 cut(s)	863
AsuC2I	CCSGG	3 cut(s)	383, 1320, 1468
AsuHPI	GGTGA	8 cut(s)	18, 140, 371, 450, 713, 1283, 1939, 2380
AsuII	TTCGAA	3 cut(s)	425, 1666, 1903
AvaI	CYCGRG	3 cut(s)	2234, 2473, 2504
AvaII	GGWCC	1 cut(s)	863
BaeGI	GKGCMC	1 cut(s)	1288
BalI	TGGCCA	4 cut(s)	359, 657, 1026, 1076
BanI	GGYRCC	2 cut(s)	620, 947
BanII	GRGCYC	1 cut(s)	1722
BbsI	GAAGAC	3 cut(s)	242, 1238, 1812
Bbv12I	GWGCWC	1 cut(s)	97
BbvCI	CCTCAGC	1 cut(s)	987
BbvI	GCAGC	6 cut(s)	344, 566, 1549, 1591, 2321, 2411
BccI	CCATC	4 cut(s)	938, 1919, 2006, 2026
BciT130I	CCWGG	3 cut(s)	81, 1213, 2076
BclI	TGATCA	2 cut(s)	1171, 1728
BcnI	CCSGG	3 cut(s)	383, 1320, 1468
BcoDI	GTCTC	3 cut(s)	698, 1327, 1381
BfaI	CTAG	3 cut(s)	450, 635, 1694
BfmI	CTRYAG	8 cut(s)	1236, 1563, 2304, 2328, 2334, 2419, 2425, 2446
BisI	GCNGC	8 cut(s)	333, 555, 1563, 1605, 2184, 2310, 2425, 2436
BlsI	GCNGC	8 cut(s)	334, 556, 1564, 1606, 2185, 2311, 2426, 2437
Bme1390I	CCNGG	6 cut(s)	81, 383, 1213, 1320, 1468, 2076
Bme18I	GGWCC	1 cut(s)	863
BmeT110I	CYCGRG	3 cut(s)	2234, 2473, 2504
BmgT120I	GGNCC	1 cut(s)	863
BmiI	GGNNCC	5 cut(s)	622, 949, 1401, 1684, 1721
BmrFI	CCNGG	6 cut(s)	81, 383, 1213, 1320, 1468, 2076
BmrI	ACTGGG	2 cut(s)	336, 1752
BmsI	GCATC	9 cut(s)	706, 749, 1128, 1305, 1753, 2197, 2219, 2362, 2518
BmuI	ACTGGG	2 cut(s)	336, 1752
BoxI	GACNNNNGTC	1 cut(s)	566
BpiI	GAAGAC	3 cut(s)	242, 1238, 1812
BpmI	CTGGAG	1 cut(s)	804
Bpu10I	CCTNAGC	1 cut(s)	987
Bpu14I	TTCGAA	3 cut(s)	425, 1666, 1903
BpuEI	CTTGAG	4 cut(s)	218, 1363, 1562, 2068
BpuMI	CCSGG	3 cut(s)	383, 1320, 1468
BsaAI	YACGTR	1 cut(s)	1937
BsaHI	GRCGYC	2 cut(s)	190, 1537
BsaJI	CCNNGG	7 cut(s)	128, 844, 1212, 1845, 1851, 2075, 2169
BsaWI	WCCGGW	1 cut(s)	1774
Bsc4I	CCNNNNNNNGG	7 cut(s)	129, 199, 292, 938, 939, 957, 2397
Bse118I	RCCGGY	2 cut(s)	363, 2531
Bse1I	ACTGG	5 cut(s)	331, 658, 1268, 1621, 1747
Bse3DI	GCAATG	1 cut(s)	342
BseAI	TCCGGA	1 cut(s)	1774
BseBI	CCWGG	3 cut(s)	81, 1213, 2076
BseDI	CCNNGG	7 cut(s)	128, 844, 1212, 1845, 1851, 2075, 2169
BseLI	CCNNNNNNNGG	7 cut(s)	129, 199, 292, 938, 939, 957, 2397
BseMI	GCAATG	1 cut(s)	342
BseMII	CTCAG	4 cut(s)	516, 978, 1356, 2550
BseNI	ACTGG	5 cut(s)	331, 658, 1268, 1621, 1747
BseRI	GAGGAG	4 cut(s)	506, 973, 1416, 1874
BseSI	GKGCMC	1 cut(s)	1288
BseXI	GCAGC	6 cut(s)	344, 566, 1549, 1591, 2321, 2411
Bsh1236I	CGCG	3 cut(s)	177, 846, 2295
BshFI	GGCC	8 cut(s)	84, 359, 657, 1026, 1076, 1552, 1850, 2285
BshNI	GGYRCC	2 cut(s)	620, 947
BsiHKAI	GWGCWC	1 cut(s)	97
BsiHKCI	CYCGRG	3 cut(s)	2234, 2473, 2504
BsiSI	CCGG	6 cut(s)	364, 383, 1320, 1467, 1775, 2532
BslFI	GGGAC	3 cut(s)	497, 1057, 2494
BslI	CCNNNNNNNGG	7 cut(s)	129, 199, 292, 938, 939, 957, 2397
BsmAI	GTCTC	3 cut(s)	698, 1327, 1381
BsmFI	GGGAC	3 cut(s)	497, 1057, 2494
BsmI	GAATGC	1 cut(s)	1911
BsnI	GGCC	8 cut(s)	84, 359, 657, 1026, 1076, 1552, 1850, 2285
BsoBI	CYCGRG	3 cut(s)	2234, 2473, 2504
Bsp119I	TTCGAA	3 cut(s)	425, 1666, 1903
Bsp1286I	GDGCHC	3 cut(s)	97, 1288, 1722
Bsp13I	TCCGGA	1 cut(s)	1774
Bsp1407I	TGTACA	2 cut(s)	1032, 2109
Bsp143I	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
Bsp19I	CCATGG	1 cut(s)	1851
BspANI	GGCC	8 cut(s)	84, 359, 657, 1026, 1076, 1552, 1850, 2285
BspCNI	CTCAG	4 cut(s)	517, 979, 1357, 2549
BspEI	TCCGGA	1 cut(s)	1774
BspFNI	CGCG	3 cut(s)	177, 846, 2295
BspHI	TCATGA	1 cut(s)	733
BspLI	GGNNCC	5 cut(s)	622, 949, 1401, 1684, 1721
BspMAI	CTGCAG	2 cut(s)	1567, 2429
BspPI	GGATC	3 cut(s)	31, 256, 1554
BspQI	GCTCTTC	1 cut(s)	2154
BspT104I	TTCGAA	3 cut(s)	425, 1666, 1903
BspT107I	GGYRCC	2 cut(s)	620, 947
BsrBI	CCGCTC	1 cut(s)	1204
BsrDI	GCAATG	1 cut(s)	342
BsrFI	RCCGGY	2 cut(s)	363, 2531
BsrGI	TGTACA	2 cut(s)	1032, 2109
BsrI	ACTGG	5 cut(s)	331, 658, 1268, 1621, 1747
BssAI	RCCGGY	2 cut(s)	363, 2531
BssECI	CCNNGG	7 cut(s)	128, 844, 1212, 1845, 1851, 2075, 2169
BssMI	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
BssNI	GRCGYC	2 cut(s)	190, 1537
BssT1I	CCWWGG	4 cut(s)	128, 1845, 1851, 2169
Bst2UI	CCWGG	3 cut(s)	81, 1213, 2076
Bst4CI	ACNGT	3 cut(s)	974, 1186, 2450
Bst6I	CTCTTC	5 cut(s)	240, 933, 1212, 1347, 2154
BstACI	GRCGYC	2 cut(s)	190, 1537
BstAUI	TGTACA	2 cut(s)	1032, 2109
BstBAI	YACGTR	1 cut(s)	1937
BstBI	TTCGAA	3 cut(s)	425, 1666, 1903
BstC8I	GCNNGC	3 cut(s)	337, 1117, 2166
BstDEI	CTNAG	7 cut(s)	525, 907, 987, 1251, 1365, 1895, 2536
BstDSI	CCRYGG	2 cut(s)	844, 1851
BstFNI	CGCG	3 cut(s)	177, 846, 2295
BstHHI	GCGC	1 cut(s)	59
BstKTI	GATC	8 cut(s)	26, 181, 257, 264, 1174, 1455, 1549, 1731
BstMAI	GTCTC	3 cut(s)	698, 1327, 1381
BstMBI	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
BstMWI	GCNNNNNNNGC	3 cut(s)	409, 779, 2346
BstNI	CCWGG	3 cut(s)	81, 1213, 2076
BstNSI	RCATGY	2 cut(s)	645, 1119
BstPAI	GACNNNNGTC	1 cut(s)	566
BstSCI	CCNGG	6 cut(s)	79, 381, 1211, 1318, 1466, 2074
BstSFI	CTRYAG	8 cut(s)	1236, 1563, 2304, 2328, 2334, 2419, 2425, 2446
BstSLI	GKGCMC	1 cut(s)	1288
BstUI	CGCG	3 cut(s)	177, 846, 2295
BstV1I	GCAGC	6 cut(s)	344, 566, 1549, 1591, 2321, 2411
BstV2I	GAAGAC	3 cut(s)	242, 1238, 1812
BstXI	CCANNNNNNTGG	1 cut(s)	1852
BsuRI	GGCC	8 cut(s)	84, 359, 657, 1026, 1076, 1552, 1850, 2285
BtgI	CCRYGG	2 cut(s)	844, 1851
BtgZI	GCGATG	1 cut(s)	2385
BtsI	GCAGTG	2 cut(s)	1286, 1423
BtsIMutI	CAGTG	5 cut(s)	522, 651, 1286, 1423, 1797
Cac8I	GCNNGC	3 cut(s)	337, 1117, 2166
CciI	TCATGA	1 cut(s)	733
CfoI	GCGC	1 cut(s)	59
Cfr10I	RCCGGY	2 cut(s)	363, 2531
Cfr13I	GGNCC	1 cut(s)	863
Cfr42I	CCGCGG	1 cut(s)	847
CseI	GACGC	2 cut(s)	198, 1526
Csp6I	GTAC	4 cut(s)	1033, 1246, 1484, 2110
CspCI	CAANNNNNGTGG	4 cut(s)	1290, 1325, 1399, 1434
CviQI	GTAC	4 cut(s)	1033, 1246, 1484, 2110
DdeI	CTNAG	7 cut(s)	525, 907, 987, 1251, 1365, 1895, 2536
DpnI	GATC	8 cut(s)	25, 180, 256, 263, 1173, 1454, 1548, 1730
DpnII	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
EaeI	YGGCCR	4 cut(s)	357, 655, 1024, 1074
Eam1104I	CTCTTC	5 cut(s)	240, 933, 1212, 1347, 2154
EarI	CTCTTC	5 cut(s)	240, 933, 1212, 1347, 2154
EciI	GGCGGA	1 cut(s)	87
Eco130I	CCWWGG	4 cut(s)	128, 1845, 1851, 2169
Eco24I	GRGCYC	1 cut(s)	1722
Eco47I	GGWCC	1 cut(s)	863
Eco57I	CTGAAG	4 cut(s)	1371, 1821, 2433, 2452
Eco88I	CYCGRG	3 cut(s)	2234, 2473, 2504
EcoRI	GAATTC	1 cut(s)	1874
EcoRII	CCWGG	3 cut(s)	79, 1211, 2074
EcoT14I	CCWWGG	4 cut(s)	128, 1845, 1851, 2169
EcoT22I	ATGCAT	3 cut(s)	549, 1121, 2212
EcoT38I	GRGCYC	1 cut(s)	1722
ErhI	CCWWGG	4 cut(s)	128, 1845, 1851, 2169
FalI	AAGNNNNNCTT	4 cut(s)	1524, 1556, 2030, 2062
FaqI	GGGAC	3 cut(s)	497, 1057, 2494
FauI	CCCGC	4 cut(s)	77, 160, 851, 2300
FauNDI	CATATG	2 cut(s)	808, 820
FbaI	TGATCA	2 cut(s)	1171, 1728
FblI	GTMKAC	3 cut(s)	570, 1621, 2418
Fnu4HI	GCNGC	8 cut(s)	333, 555, 1563, 1605, 2184, 2310, 2425, 2436
FriOI	GRGCYC	1 cut(s)	1722
Fsp4HI	GCNGC	8 cut(s)	333, 555, 1563, 1605, 2184, 2310, 2425, 2436
FspBI	CTAG	3 cut(s)	450, 635, 1694
FspI	TGCGCA	1 cut(s)	58
GlaI	GCGC	1 cut(s)	58
GluI	GCNGC	8 cut(s)	333, 555, 1563, 1605, 2184, 2310, 2425, 2436
GsuI	CTGGAG	1 cut(s)	804
HaeIII	GGCC	8 cut(s)	84, 359, 657, 1026, 1076, 1552, 1850, 2285
HapII	CCGG	6 cut(s)	364, 383, 1320, 1467, 1775, 2532
HgaI	GACGC	2 cut(s)	198, 1526
HhaI	GCGC	1 cut(s)	59
Hin1I	GRCGYC	2 cut(s)	190, 1537
Hin6I	GCGC	1 cut(s)	57
HinP1I	GCGC	1 cut(s)	57
HincII	GTYRAC	5 cut(s)	508, 571, 1182, 1978, 2356
HindII	GTYRAC	5 cut(s)	508, 571, 1182, 1978, 2356
HindIII	AAGCTT	1 cut(s)	410
HpaI	GTTAAC	1 cut(s)	1978
HpaII	CCGG	6 cut(s)	364, 383, 1320, 1467, 1775, 2532
HphI	GGTGA	8 cut(s)	18, 140, 371, 450, 713, 1283, 1939, 2380
Hpy188I	TCNGA	9 cut(s)	166, 432, 1111, 1176, 1201, 1366, 1575, 1921, 2413
HpyAV	CCTTC	6 cut(s)	452, 807, 1045, 1416, 1563, 2386
HpyCH4III	ACNGT	3 cut(s)	974, 1186, 2450
HpyCH4IV	ACGT	1 cut(s)	1936
HpyF10VI	GCNNNNNNNGC	3 cut(s)	409, 779, 2346
HpyF3I	CTNAG	7 cut(s)	525, 907, 987, 1251, 1365, 1895, 2536
HpySE526I	ACGT	1 cut(s)	1936
Hsp92I	GRCGYC	2 cut(s)	190, 1537
HspAI	GCGC	1 cut(s)	57
Kpn2I	TCCGGA	1 cut(s)	1774
Ksp22I	TGATCA	2 cut(s)	1171, 1728
KspAI	GTTAAC	1 cut(s)	1978
KspI	CCGCGG	1 cut(s)	847
Kzo9I	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
LguI	GCTCTTC	1 cut(s)	2154
LmnI	GCTCC	5 cut(s)	100, 785, 960, 1725, 1861
Lsp1109I	GCAGC	6 cut(s)	344, 566, 1549, 1591, 2321, 2411
LweI	GCATC	9 cut(s)	706, 749, 1128, 1305, 1753, 2197, 2219, 2362, 2518
MaeI	CTAG	3 cut(s)	450, 635, 1694
MaeII	ACGT	1 cut(s)	1936
MaeIII	GTNAC	7 cut(s)	10, 377, 456, 584, 649, 1612, 1937
MalI	GATC	8 cut(s)	25, 180, 256, 263, 1173, 1454, 1548, 1730
MbiI	CCGCTC	1 cut(s)	1204
MboI	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
MfeI	CAATTG	1 cut(s)	2154
MhlI	GDGCHC	3 cut(s)	97, 1288, 1722
MlsI	TGGCCA	4 cut(s)	359, 657, 1026, 1076
MluNI	TGGCCA	4 cut(s)	359, 657, 1026, 1076
MlyI	GAGTC	6 cut(s)	536, 1100, 1376, 2264, 2496, 2517
MmeI	TCCRAC	1 cut(s)	779
Mox20I	TGGCCA	4 cut(s)	359, 657, 1026, 1076
Mph1103I	ATGCAT	3 cut(s)	549, 1121, 2212
MroI	TCCGGA	1 cut(s)	1774
MroXI	GAANNNNTTC	4 cut(s)	1002, 1662, 1874, 2228
MscI	TGGCCA	4 cut(s)	359, 657, 1026, 1076
MseI	TTAA	5 cut(s)	278, 477, 1581, 1599, 1977
MslI	CAYNNNNRTG	2 cut(s)	942, 1341
Msp20I	TGGCCA	4 cut(s)	359, 657, 1026, 1076
MspA1I	CMGCKG	4 cut(s)	335, 846, 2312, 2424
MspI	CCGG	6 cut(s)	364, 383, 1320, 1467, 1775, 2532
MspR9I	CCNGG	6 cut(s)	81, 383, 1213, 1320, 1468, 2076
MunI	CAATTG	1 cut(s)	2154
Mva1269I	GAATGC	1 cut(s)	1911
MvaI	CCWGG	3 cut(s)	81, 1213, 2076
MvnI	CGCG	3 cut(s)	177, 846, 2295
MwoI	GCNNNNNNNGC	3 cut(s)	409, 779, 2346
NciI	CCSGG	3 cut(s)	383, 1320, 1468
NcoI	CCATGG	1 cut(s)	1851
NdeI	CATATG	2 cut(s)	808, 820
NdeII	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
NlaIV	GGNNCC	5 cut(s)	622, 949, 1401, 1684, 1721
NmuCI	GTSAC	5 cut(s)	10, 377, 456, 649, 1612
NsbI	TGCGCA	1 cut(s)	58
NsiI	ATGCAT	3 cut(s)	549, 1121, 2212
NspI	RCATGY	2 cut(s)	645, 1119
NspV	TTCGAA	3 cut(s)	425, 1666, 1903
PaeI	GCATGC	1 cut(s)	1119
PaeR7I	CTCGAG	1 cut(s)	2504
PagI	TCATGA	1 cut(s)	733
PciSI	GCTCTTC	1 cut(s)	2154
PctI	GAATGC	1 cut(s)	1911
PdmI	GAANNNNTTC	4 cut(s)	1002, 1662, 1874, 2228
PfeI	GAWTC	6 cut(s)	427, 889, 1255, 1513, 1643, 2098
PflFI	GACNNNGTC	1 cut(s)	503
PflMI	CCANNNNNTGG	1 cut(s)	2397
PkrI	GCNGC	8 cut(s)	334, 556, 1564, 1606, 2185, 2311, 2426, 2437
PleI	GAGTC	6 cut(s)	535, 1100, 1375, 2263, 2496, 2517
PpsI	GAGTC	6 cut(s)	535, 1100, 1375, 2263, 2496, 2517
Ppu21I	YACGTR	1 cut(s)	1937
PshAI	GACNNNNGTC	1 cut(s)	566
PsiI	TTATAA	3 cut(s)	836, 1443, 1965
Psp6I	CCWGG	3 cut(s)	79, 1211, 2074
PspGI	CCWGG	3 cut(s)	79, 1211, 2074
PspN4I	GGNNCC	5 cut(s)	622, 949, 1401, 1684, 1721
PspPI	GGNCC	1 cut(s)	863
PspXI	VCTCGAGB	1 cut(s)	2504
PstI	CTGCAG	2 cut(s)	1567, 2429
PsyI	GACNNNGTC	1 cut(s)	503
PvuII	CAGCTG	3 cut(s)	335, 2312, 2424
RsaI	GTAC	4 cut(s)	1034, 1247, 1485, 2111
RsaNI	GTAC	4 cut(s)	1033, 1246, 1484, 2110
RseI	CAYNNNNRTG	2 cut(s)	942, 1341
SacII	CCGCGG	1 cut(s)	847
SalI	GTCGAC	1 cut(s)	569
SapI	GCTCTTC	1 cut(s)	2154
SaqAI	TTAA	5 cut(s)	278, 477, 1581, 1599, 1977
SatI	GCNGC	8 cut(s)	333, 555, 1563, 1605, 2184, 2310, 2425, 2436
Sau3AI	GATC	8 cut(s)	23, 178, 254, 261, 1171, 1452, 1546, 1728
Sau96I	GGNCC	1 cut(s)	863
SchI	GAGTC	6 cut(s)	536, 1100, 1376, 2264, 2496, 2517
ScrFI	CCNGG	6 cut(s)	81, 383, 1213, 1320, 1468, 2076
SduI	GDGCHC	3 cut(s)	97, 1288, 1722
SfaNI	GCATC	9 cut(s)	706, 749, 1128, 1305, 1753, 2197, 2219, 2362, 2518
SfcI	CTRYAG	8 cut(s)	1236, 1563, 2304, 2328, 2334, 2419, 2425, 2446
Sfr274I	CTCGAG	1 cut(s)	2504
Sfr303I	CCGCGG	1 cut(s)	847
SfuI	TTCGAA	3 cut(s)	425, 1666, 1903
SgrBI	CCGCGG	1 cut(s)	847
SinI	GGWCC	1 cut(s)	863
SlaI	CTCGAG	1 cut(s)	2504
SmiMI	CAYNNNNRTG	2 cut(s)	942, 1341
SmlI	CTYRAG	5 cut(s)	197, 1378, 1541, 2047, 2504
SmoI	CTYRAG	5 cut(s)	197, 1378, 1541, 2047, 2504
SphI	GCATGC	1 cut(s)	1119
SspI	AATATT	2 cut(s)	1039, 1579
SspMI	CTAG	3 cut(s)	450, 635, 1694
StyD4I	CCNGG	6 cut(s)	79, 381, 1211, 1318, 1466, 2074
StyI	CCWWGG	4 cut(s)	128, 1845, 1851, 2169
TaaI	ACNGT	3 cut(s)	974, 1186, 2450
TaiI	ACGT	1 cut(s)	1939
TatI	WGTACW	3 cut(s)	1032, 1483, 2109
TauI	GCSGC	2 cut(s)	2186, 2438
TfiI	GAWTC	6 cut(s)	427, 889, 1255, 1513, 1643, 2098
Tru1I	TTAA	5 cut(s)	278, 477, 1581, 1599, 1977
Tru9I	TTAA	5 cut(s)	278, 477, 1581, 1599, 1977
TscAI	CASTG	5 cut(s)	529, 658, 1293, 1423, 1804
TseFI	GTSAC	5 cut(s)	10, 377, 456, 649, 1612
TseI	GCWGC	6 cut(s)	332, 554, 1562, 1604, 2309, 2424
Tsp45I	GTSAC	5 cut(s)	10, 377, 456, 649, 1612
TspRI	CASTG	5 cut(s)	529, 658, 1293, 1423, 1804
Tth111I	GACNNNGTC	1 cut(s)	503
Van91I	CCANNNNNTGG	1 cut(s)	2397
VpaK11BI	GGWCC	1 cut(s)	863
XapI	RAATTY	2 cut(s)	31, 1874
XbaI	TCTAGA	1 cut(s)	634
XceI	RCATGY	2 cut(s)	645, 1119
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2136
XhoI	CTCGAG	1 cut(s)	2504
XmiI	GTMKAC	3 cut(s)	570, 1621, 2418
XmnI	GAANNNNTTC	4 cut(s)	1002, 1662, 1874, 2228
XspI	CTAG	3 cut(s)	450, 635, 1694
Zsp2I	ATGCAT	3 cut(s)	549, 1121, 2212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.