Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013599

Physical Location & Seq

Genomic Coordinates Forward (+)

34079447 .. 34079857

Gene Structure 411 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1254100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 411 bp

ATGGAAGTCATTCGAATGCTGCTTGAAAATGGTGTGGATATGGATGCTAAAGATGAGGATGGATACACCGCGTTGCACTGTGCAGCGGAGTCGATACATGATGATGTGATTGAGATGTTGGTAAAGAAAGGAGCTGATGCTGAAGCTCGAACTAACAAAGATGTAACTACACTGCAGATTGCTGAGTCTTTGCACTACGTTGGGATTACTAGGATACTTATTCATAAAGGTGCAACAAAAGACAATAGTAGTACGGTACATATCTTGACCCATGCCTCAATTTCATTTGGGAAAAAATCTATGGGACTAGAGGAGGAGATGACCAAAGGAGGAATGAAGAAGAAGAAATCGAGTCAAGCTAGAGCTCTTTGTGGGAGCTTCGATCGTTTGATGCCATTGAATGTTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

137

Amino Acids

14.89

Weight (kDa)

6.29

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	2 - 81	6.5e-12	Ankyrin repeats (3 copies)
Ank_4	PF13637	2 - 29	2.2e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	7 - 61	1.2e-06	Ankyrin repeats (many copies)
Ank	PF00023	20 - 52	4.8e-06	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013599)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g28430
malus_domestica	MD03G1254100.v1.1 MD08G1160600.v1.1 MD12G1008200.v1.1 MD12G1165300.v1.1 MD14G1005600.v1.1
prunus_persica	Prupe.7G009400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0496481
rosa_laevigata	RLG00000022523
rosa_multiflora	Rmu_co8439577.1_g000001 Rmu_sc0001127.1_g000020
rosa_roxburghii	Rroxscaffold_6G00389080
rosa_rugosa	Rorug03G0283200
rosa_samantha	Rh3AG329200 Rh3BG365000 Rh3CG362300 Rh3DG365300
rosa_wichuraiana	Rw3G028800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	71
AciI	CCGC	2 cut(s)	69, 86
AcuI	CTGAAG	1 cut(s)	162
AfaI	GTAC	2 cut(s)	253, 258
AgsI	TTSAA	2 cut(s)	26, 400
AluBI	AGCT	5 cut(s)	134, 146, 359, 365, 378
AluI	AGCT	5 cut(s)	134, 146, 359, 365, 378
Alw21I	GWGCWC	1 cut(s)	367
ApeKI	GCWGC	2 cut(s)	19, 83
Asp700I	GAANNNNTTC	1 cut(s)	9
AsuII	TTCGAA	1 cut(s)	13
BanII	GRGCYC	1 cut(s)	367
Bbv12I	GWGCWC	1 cut(s)	367
BbvI	GCAGC	2 cut(s)	6, 95
BccI	CCATC	1 cut(s)	53
BcgI	CGANNNNNNTGC	2 cut(s)	72, 106
BciVI	GTATCC	2 cut(s)	56, 207
BfaI	CTAG	3 cut(s)	210, 308, 360
BfmI	CTRYAG	1 cut(s)	173
BfuI	GTATCC	2 cut(s)	56, 207
BisI	GCNGC	2 cut(s)	20, 84
BlsI	GCNGC	2 cut(s)	21, 85
BmsI	GCATC	3 cut(s)	34, 127, 381
Bpu14I	TTCGAA	1 cut(s)	13
BseGI	GGATG	2 cut(s)	49, 64
BseMII	CTCAG	1 cut(s)	174
BseRI	GAGGAG	2 cut(s)	326, 329
BseXI	GCAGC	2 cut(s)	6, 95
BsgI	GTGCAG	1 cut(s)	102
Bsh1236I	CGCG	1 cut(s)	71
Bsh1285I	CGRYCG	1 cut(s)	385
BsiEI	CGRYCG	1 cut(s)	385
BsiHKAI	GWGCWC	1 cut(s)	367
BslFI	GGGAC	1 cut(s)	318
BsmFI	GGGAC	1 cut(s)	318
BsmI	GAATGC	1 cut(s)	21
Bsp119I	TTCGAA	1 cut(s)	13
Bsp1286I	GDGCHC	1 cut(s)	367
Bsp143I	GATC	1 cut(s)	382
BspACI	CCGC	2 cut(s)	69, 86
BspCNI	CTCAG	1 cut(s)	175
BspFNI	CGCG	1 cut(s)	71
BspMAI	CTGCAG	1 cut(s)	177
BspT104I	TTCGAA	1 cut(s)	13
BssMI	GATC	1 cut(s)	382
Bst4CI	ACNGT	2 cut(s)	80, 256
BstBI	TTCGAA	1 cut(s)	13
BstDEI	CTNAG	1 cut(s)	183
BstF5I	GGATG	2 cut(s)	49, 64
BstFNI	CGCG	1 cut(s)	71
BstKTI	GATC	1 cut(s)	385
BstMBI	GATC	1 cut(s)	382
BstMCI	CGRYCG	1 cut(s)	385
BstSFI	CTRYAG	1 cut(s)	173
BstUI	CGCG	1 cut(s)	71
BstV1I	GCAGC	2 cut(s)	6, 95
BsuI	GTATCC	2 cut(s)	56, 207
BtsCI	GGATG	2 cut(s)	49, 64
BtsI	GCAGTG	1 cut(s)	170
BtsIMutI	CAGTG	2 cut(s)	76, 170
Csp6I	GTAC	2 cut(s)	252, 257
CviAII	CATG	2 cut(s)	98, 272
CviJI	RGCY	5 cut(s)	134, 146, 359, 365, 378
CviKI_1	RGCY	5 cut(s)	134, 146, 359, 365, 378
CviQI	GTAC	2 cut(s)	252, 257
DdeI	CTNAG	1 cut(s)	183
DpnI	GATC	1 cut(s)	384
DpnII	GATC	1 cut(s)	382
Ecl136II	GAGCTC	1 cut(s)	365
Eco24I	GRGCYC	1 cut(s)	367
Eco53kI	GAGCTC	1 cut(s)	365
Eco57I	CTGAAG	1 cut(s)	162
EcoICRI	GAGCTC	1 cut(s)	365
EcoT38I	GRGCYC	1 cut(s)	367
FaeI	CATG	2 cut(s)	101, 275
FaiI	YATR	6 cut(s)	41, 99, 225, 261, 273, 302
FaqI	GGGAC	1 cut(s)	318
FatI	CATG	2 cut(s)	97, 271
Fnu4HI	GCNGC	2 cut(s)	20, 84
FokI	GGATG	2 cut(s)	56, 71
FriOI	GRGCYC	1 cut(s)	367
Fsp4HI	GCNGC	2 cut(s)	20, 84
FspBI	CTAG	3 cut(s)	210, 308, 360
GluI	GCNGC	2 cut(s)	20, 84
Hin1II	CATG	2 cut(s)	101, 275
HinfI	GANTC	3 cut(s)	89, 185, 352
Hpy188III	TCNNGA	1 cut(s)	265
HpyCH4III	ACNGT	2 cut(s)	80, 256
HpyCH4IV	ACGT	1 cut(s)	198
HpyCH4V	TGCA	5 cut(s)	76, 83, 175, 193, 233
HpyF3I	CTNAG	1 cut(s)	183
HpySE526I	ACGT	1 cut(s)	198
Hsp92II	CATG	2 cut(s)	101, 275
Kzo9I	GATC	1 cut(s)	382
LmnI	GCTCC	2 cut(s)	131, 375
Lsp1109I	GCAGC	2 cut(s)	6, 95
LweI	GCATC	3 cut(s)	34, 127, 381
MaeI	CTAG	3 cut(s)	210, 308, 360
MaeII	ACGT	1 cut(s)	198
MaeIII	GTNAC	1 cut(s)	163
MalI	GATC	1 cut(s)	384
MboI	GATC	1 cut(s)	382
MboII	GAAGA	3 cut(s)	349, 352, 355
MhlI	GDGCHC	1 cut(s)	367
MluCI	AATT	1 cut(s)	279
MlyI	GAGTC	3 cut(s)	98, 194, 361
MnlI	CCTC	5 cut(s)	49, 286, 304, 307, 323
MroXI	GAANNNNTTC	1 cut(s)	9
MslI	CAYNNNNRTG	3 cut(s)	14, 102, 228
MspA1I	CMGCKG	1 cut(s)	86
Mva1269I	GAATGC	1 cut(s)	21
MvnI	CGCG	1 cut(s)	71
NdeII	GATC	1 cut(s)	382
NlaIII	CATG	2 cut(s)	101, 275
NspV	TTCGAA	1 cut(s)	13
PctI	GAATGC	1 cut(s)	21
PdmI	GAANNNNTTC	1 cut(s)	9
PkrI	GCNGC	2 cut(s)	21, 85
Ple19I	CGATCG	1 cut(s)	385
PleI	GAGTC	3 cut(s)	97, 193, 360
PpsI	GAGTC	3 cut(s)	97, 193, 360
Psp124BI	GAGCTC	1 cut(s)	367
PstI	CTGCAG	1 cut(s)	177
PvuI	CGATCG	1 cut(s)	385
RsaI	GTAC	2 cut(s)	253, 258
RsaNI	GTAC	2 cut(s)	252, 257
RseI	CAYNNNNRTG	3 cut(s)	14, 102, 228
SacI	GAGCTC	1 cut(s)	367
SatI	GCNGC	2 cut(s)	20, 84
Sau3AI	GATC	1 cut(s)	382
SchI	GAGTC	3 cut(s)	98, 194, 361
SduI	GDGCHC	1 cut(s)	367
SetI	ASST	7 cut(s)	136, 148, 201, 232, 361, 367, 380
SfaNI	GCATC	3 cut(s)	34, 127, 381
SfcI	CTRYAG	1 cut(s)	173
SfuI	TTCGAA	1 cut(s)	13
SmiMI	CAYNNNNRTG	3 cut(s)	14, 102, 228
Sse9I	AATT	1 cut(s)	279
SsiI	CCGC	2 cut(s)	69, 86
SspMI	CTAG	3 cut(s)	210, 308, 360
SstI	GAGCTC	1 cut(s)	367
TaaI	ACNGT	2 cut(s)	80, 256
TaiI	ACGT	1 cut(s)	201
TaqI	TCGA	5 cut(s)	13, 92, 148, 350, 381
TasI	AATT	1 cut(s)	279
TscAI	CASTG	2 cut(s)	83, 177
TseI	GCWGC	2 cut(s)	19, 83
TspDTI	ATGAA	3 cut(s)	212, 273, 350
TspRI	CASTG	2 cut(s)	83, 177
XmnI	GAANNNNTTC	1 cut(s)	9
XspI	CTAG	3 cut(s)	210, 308, 360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.