MD08G1160600.v1.1

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013599
Physical Location & Seq
Forward (+)
18571737 .. 18572129
393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1160600.v1.1.491

Sequence Viewer

Length: 393 bp
ATGTCAGCCGCGGCAATCAATGGAAGAGACCTACATGGGTGGACTGCTCTCCATAAAGCCTGTTTCAAAGGGAAGATAAAAGTCATTCGAACACTGCTTGAAAAGGGTGTGGATATAGATGCTAAAGATAAGGTTGGATACACCGCGTTGCATTGTGCAGCGGAGTCGGGACATGTAGATGTGATTAAGATGTTGCTAAAGAAAGGAGTTGATGTTGAAGCTCTAACTAACAAAGGTGTGACTGCACTGCAGATTGCTGAGTCTTTGCACTACGTTAGGATTACTAGGATACTTATTCATAGAGGTGCAACAAAAGACAATAGTAATATGGCACATATCTTGACCCATGCCTCAATTTCATTTGGGAAAAAAATCTATGGAACTAGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

131

Amino Acids

14.11

Weight (kDa)

9.77

Isoelectric Point (pI)

16.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 6 - 43 7.1e-07 Ankyrin repeats (3 copies)
Ank PF00023 12 - 44 4.9e-08 Ankyrin repeat
Ank_3 PF13606 12 - 41 8.6e-07 Ankyrin repeat
Ank_4 PF13637 17 - 66 4.6e-15 Ankyrin repeats (many copies)
Ank_5 PF13857 32 - 86 5e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 41 - 107 1.7e-12 Ankyrin repeats (3 copies)
Ank PF00023 46 - 77 1.7e-09 Ankyrin repeat
Ank_3 PF13606 46 - 73 1e-07 Ankyrin repeat
Ank_4 PF13637 50 - 86 3.4e-08 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 11, 146
AciI CCGC 4 cut(s) 9, 11, 144, 161
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 3 cut(s) 67, 101, 218
AluBI AGCT 1 cut(s) 221
AluI AGCT 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 21
ApeKI GCWGC 1 cut(s) 158
AsuII TTCGAA 1 cut(s) 88
BbvI GCAGC 1 cut(s) 170
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciVI GTATCC 2 cut(s) 131, 282
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 2 cut(s) 285, 384
BfmI CTRYAG 1 cut(s) 248
BfuI GTATCC 2 cut(s) 131, 282
BisI GCNGC 3 cut(s) 9, 12, 159
BlsI GCNGC 3 cut(s) 10, 13, 160
BmsI GCATC 1 cut(s) 109
Bpu14I TTCGAA 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
BseDI CCNNGG 1 cut(s) 9
BseMII CTCAG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 170
BsgI GTGCAG 2 cut(s) 177, 228
Bsh1236I CGCG 2 cut(s) 11, 146
BslFI GGGAC 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 183
Bso31I GGTCTC 1 cut(s) 21
Bsp119I TTCGAA 1 cut(s) 88
BspACI CCGC 4 cut(s) 9, 11, 144, 161
BspCNI CTCAG 1 cut(s) 250
BspFNI CGCG 2 cut(s) 11, 146
BspMAI CTGCAG 1 cut(s) 252
BspT104I TTCGAA 1 cut(s) 88
BspTNI GGTCTC 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 9
Bst6I CTCTTC 1 cut(s) 19
BstBI TTCGAA 1 cut(s) 88
BstDEI CTNAG 1 cut(s) 258
BstDSI CCRYGG 1 cut(s) 9
BstFNI CGCG 2 cut(s) 11, 146
BstMAI GTCTC 1 cut(s) 21
BstNSI RCATGY 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 248
BstUI CGCG 2 cut(s) 11, 146
BstV1I GCAGC 1 cut(s) 170
BsuI GTATCC 2 cut(s) 131, 282
BtgI CCRYGG 1 cut(s) 9
BtsI GCAGTG 2 cut(s) 92, 245
BtsIMutI CAGTG 2 cut(s) 92, 245
Cfr42I CCGCGG 1 cut(s) 12
CviAII CATG 3 cut(s) 35, 173, 347
CviJI RGCY 3 cut(s) 8, 59, 221
CviKI_1 RGCY 3 cut(s) 8, 59, 221
DdeI CTNAG 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 21
FaeI CATG 3 cut(s) 38, 176, 350
FaiI YATR 9 cut(s) 36, 54, 116, 174, 300, 329, 336, 348, 378
FaqI GGGAC 1 cut(s) 183
FatI CATG 3 cut(s) 34, 172, 346
Fnu4HI GCNGC 3 cut(s) 9, 12, 159
Fsp4HI GCNGC 3 cut(s) 9, 12, 159
FspBI CTAG 2 cut(s) 285, 384
GluI GCNGC 3 cut(s) 9, 12, 159
Hin1II CATG 3 cut(s) 38, 176, 350
HinfI GANTC 2 cut(s) 164, 260
Hpy166II GTNNAC 1 cut(s) 42
Hpy188III TCNNGA 2 cut(s) 168, 340
Hpy8I GTNNAC 1 cut(s) 42
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 6 cut(s) 151, 158, 245, 250, 268, 308
HpyF3I CTNAG 1 cut(s) 258
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 3 cut(s) 38, 176, 350
KspI CCGCGG 1 cut(s) 12
LpnPI CCDG 1 cut(s) 73
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 1 cut(s) 109
MaeI CTAG 2 cut(s) 285, 384
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 238
MboII GAAGA 2 cut(s) 36, 85
MluCI AATT 1 cut(s) 354
MlyI GAGTC 2 cut(s) 173, 269
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 3 cut(s) 296, 361, 380
MseI TTAA 1 cut(s) 186
MslI CAYNNNNRTG 2 cut(s) 177, 303
MspA1I CMGCKG 2 cut(s) 11, 161
MvnI CGCG 2 cut(s) 11, 146
NlaIII CATG 3 cut(s) 38, 176, 350
NmuCI GTSAC 1 cut(s) 238
NspI RCATGY 1 cut(s) 176
NspV TTCGAA 1 cut(s) 88
PciI ACATGT 1 cut(s) 172
PkrI GCNGC 3 cut(s) 10, 13, 160
PleI GAGTC 2 cut(s) 172, 268
PpsI GAGTC 2 cut(s) 172, 268
PscI ACATGT 1 cut(s) 172
PstI CTGCAG 1 cut(s) 252
RseI CAYNNNNRTG 2 cut(s) 177, 303
SacII CCGCGG 1 cut(s) 12
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 3 cut(s) 9, 12, 159
SchI GAGTC 2 cut(s) 173, 269
SetI ASST 6 cut(s) 33, 135, 223, 238, 276, 307
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 248
Sfr303I CCGCGG 1 cut(s) 12
SfuI TTCGAA 1 cut(s) 88
SgrBI CCGCGG 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 177, 303
Sse9I AATT 1 cut(s) 354
SsiI CCGC 4 cut(s) 9, 11, 144, 161
SspMI CTAG 2 cut(s) 285, 384
TaiI ACGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 88
TasI AATT 1 cut(s) 354
TauI GCSGC 2 cut(s) 11, 14
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 2 cut(s) 99, 252
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 2 cut(s) 287, 348
TspRI CASTG 2 cut(s) 99, 252
XceI RCATGY 1 cut(s) 176
XspI CTAG 2 cut(s) 285, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.