Rroxscaffold_6G00389080

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013599
Physical Location & Seq
Reverse (-)
2457929 .. 2459578
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00389080.1

Sequence Viewer

Length: 1650 bp
ATGGACCGGCTCATAAGCTTGGAGCCATCAAATTTCGTTGCAATACGGATCGAACCAGGTCAGAAATGTTACGGCCTACTCACTTTGCGCAACGTCATGTACACCATGCCTGTGGCCTTCAGGCTTCAAGCCCTGGTCAAGAATCGCTACACGGTGAGGCCTCAATCCGGGATCATATCTCCGCTCGAGAAGCTAACCATTGAGATCGTCTATCACCTTCCACCGGGATCGGGCCTCCCGGACTCGTTCCCTTATTGCCATGACTCCTTCCTTTTGCATAGCGTGGTCGTGCCCGGAGCAGCCATCAAAAACTCTTCATCGACTTTCGATGCGGTCCCCAGTGACTGGTTCACAACAAAGAAGAAGCAAGTGTTCATAGACAGCGGTGTGAAGATTATGTTCATTGGTTCACCTGTTTTGGCACAACTTGTGTCCGATGGTTTGATGGACGATATAAGAGAAGTGCTCGAGAAGAGCGACCCTTCATGGAAAGCAGCCGACTCTGTGGATTCGGATGGTCAATCTTTACTTCACTTGGCTATTGCTCAAGGCCGACCCGACCTCGTCCAGCTGCTTCTCGAATTCGAGCCAGACGTGGAGGCTCAGAGCCGTTCGGGGTCGAGCCCGCTTGAGGCCGCGGCTGCTTCGGGTGAGGCTTTGATAGTCGAGGTTCTGTTGGCTCGCCGTGCAAGCACTGAAAGATCAGAGTCCTCCACTTGGGGACCTATCCACCTGGCAGCCGGAGCTGGCCACGTCGAAGTTCTGAGGCTTCTTATAATTAAGGGGGCTAATGTTGATGCTCAAACAAAAGACGGAAACACCGCCTTACACCTAGCAGTGGAAGAGCGCAGAAGGGACTGTGCGAGGCTTTTGCTAGCAAGTGGTGCTAAGGCCGAAGTTCGTAATAGCAAGGAGGGTGACACCCCGCTGCACATAGCCGCAGGGTTGGGAGATGAGTACATGGTCAAGCTGTTGCTGCAAAAGGGTACTGCCAATAAAGATATCAGAAACTACGCCGGTCGAACGGCTTATGATGTTGCCGCAGAGAATGGTCACACCAAGCTCTTTGACTCCCTTCGGCTCGGAGATAGCTTGTGTATAGCTGCCAGGAAAGGAGAGGTGAGAACAATTCTGAGGGTGCTGGAGACAGGGGCCGCGATCAACGGAAGAGACCAACATGGGTGGACTGCTCTGCATAGAGCGTGCTTTAAAGGCAAAGTTGACGCCGTGCGAGCGCTGCTGGAGAAGGGTGTGGATGTGGGTGCTAAAGATGAGGACGGATACACGGCATTGCACTGCGCAGCGGAATCGGGGCATGCGGATGTGATAGATATGTTGGTGAAGAAAGGAGCAGATGCTGAAGCCAGGACTAATAAGGGTGTGACTGCGCTGCAGATTGCCGAGTCTTTGCACTATGCTGGGATTACAAGGATTCTTATGACACATGGAGGTGGTGCAATAAAAGATCAGAACATGGCACAGATTCTATCTCACGCTTCAGTTCATTCGTTTGGGAAGAAATCCATGGGCGTTGAGGAAGAGATGATCAAGGGAGGGATGATGGTGAGGAAAAATCGACTCGAGCGAGAGCTCTTCGTGGTAGCTTTGATCGTTCTGTGCCATTGGCTGTGCTTTAATATTTCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

549

Amino Acids

59.49

Weight (kDa)

6.66

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 5 - 107 1.4e-07 MSP (Major sperm protein) domain
Ank_2 PF12796 164 - 230 7.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 239 - 301 6.2e-13 Ankyrin repeats (3 copies)
Ank PF00023 242 - 270 3e-06 Ankyrin repeat
Ank_4 PF13637 243 - 292 2.7e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 263 - 313 2.8e-06 Ankyrin repeats (many copies)
Ank PF00023 271 - 302 7.6e-06 Ankyrin repeat
Ank_2 PF12796 297 - 359 5.3e-09 Ankyrin repeats (3 copies)
Ank PF00023 306 - 337 2.4e-07 Ankyrin repeat
Ank_2 PF12796 365 - 455 1.8e-19 Ankyrin repeats (3 copies)
Ank_5 PF13857 381 - 432 3.1e-08 Ankyrin repeats (many copies)
Ank PF00023 393 - 424 5.4e-07 Ankyrin repeat
Ank_4 PF13637 400 - 447 7.4e-12 Ankyrin repeats (many copies)
Ank_5 PF13857 413 - 467 7.6e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 418 - 483 4.5e-10 Ankyrin repeats (3 copies)
Ank PF00023 426 - 458 5.5e-08 Ankyrin repeat
Ank_3 PF13606 426 - 454 7.2e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
Acc16I TGCGCA 2 cut(s) 89, 1300
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 1 cut(s) 184
AccII CGCG 2 cut(s) 638, 1157
AclWI GGATC 3 cut(s) 56, 179, 235
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 2 cut(s) 31, 581
AcuI CTGAAG 3 cut(s) 103, 1380, 1482
AcyI GRCGYC 1 cut(s) 1224
AfaI GTAC 3 cut(s) 101, 959, 988
AfeI AGCGCT 1 cut(s) 1236
AfiI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
AgsI TTSAA 2 cut(s) 128, 1644
AjiI CACGTC 2 cut(s) 595, 754
AjnI CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
Alw21I GWGCWC 2 cut(s) 468, 1593
Alw26I GTCTC 2 cut(s) 1139, 1164
AlwI GGATC 3 cut(s) 56, 179, 235
AlwNI CAGNNNCTG 2 cut(s) 345, 1358
Ama87I CYCGRG 3 cut(s) 185, 467, 1580
Aor51HI AGCGCT 1 cut(s) 1236
AoxI GGCC 9 cut(s) 73, 114, 158, 232, 550, 633, 748, 891, 1152
ApoI RAATTY 2 cut(s) 31, 581
AspLEI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
AspS9I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
AsuC2I CCSGG 4 cut(s) 169, 225, 239, 294
AsuHPI GGTGA 8 cut(s) 166, 206, 402, 662, 929, 1132, 1351, 1576
AsuNHI GCTAGC 1 cut(s) 874
AvaI CYCGRG 3 cut(s) 185, 467, 1580
AvaII GGWCC 3 cut(s) 4, 334, 722
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 750
BanII GRGCYC 2 cut(s) 626, 1593
Bbv12I GWGCWC 2 cut(s) 468, 1593
BccI CCATC 6 cut(s) 34, 311, 431, 439, 509, 1555
BceAI ACGGC 6 cut(s) 88, 594, 669, 1041, 1211, 1302
BcgI CGANNNNNNTGC 4 cut(s) 853, 887, 1290, 1324
BciT130I CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
BciVI GTATCC 1 cut(s) 1274
BclI TGATCA 1 cut(s) 1545
BcnI CCSGG 4 cut(s) 169, 225, 239, 294
BcoDI GTCTC 2 cut(s) 1139, 1164
BfaI CTAG 2 cut(s) 833, 875
BfmI CTRYAG 1 cut(s) 1391
BfoI RGCGCY 1 cut(s) 1238
BfuI GTATCC 1 cut(s) 1274
Bme1390I CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
Bme18I GGWCC 3 cut(s) 4, 334, 722
BmeT110I CYCGRG 3 cut(s) 185, 467, 1580
BmgBI CACGTC 2 cut(s) 595, 754
BmgT120I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
BmiI GGNNCC 4 cut(s) 24, 336, 723, 1153
BmrFI CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 3 cut(s) 319, 787, 1345
BmtI GCTAGC 1 cut(s) 878
BmuI ACTGGG 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpmI CTGGAG 2 cut(s) 1163, 1262
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 531, 650
BpuMI CCSGG 4 cut(s) 169, 225, 239, 294
BsaHI GRCGYC 1 cut(s) 1224
BsaI GGTCTC 1 cut(s) 1164
BsaJI CCNNGG 3 cut(s) 132, 636, 1524
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
Bse118I RCCGGY 2 cut(s) 6, 1016
Bse1I ACTGG 2 cut(s) 339, 350
Bse3DI GCAATG 1 cut(s) 1289
BseBI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
BseDI CCNNGG 3 cut(s) 132, 636, 1524
BseGI GGATG 4 cut(s) 520, 1261, 1327, 1563
BseLI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
BseMI GCAATG 1 cut(s) 1289
BseMII CTCAG 3 cut(s) 617, 755, 1124
BseNI ACTGG 2 cut(s) 339, 350
BseSI GKGCMC 1 cut(s) 294
BseYI CCCAGC 1 cut(s) 1418
BsgI GTGCAG 1 cut(s) 914
Bsh1236I CGCG 2 cut(s) 638, 1157
Bsh1285I CGRYCG 1 cut(s) 1021
BshFI GGCC 9 cut(s) 75, 116, 160, 234, 552, 635, 750, 893, 1154
BsiEI CGRYCG 1 cut(s) 1021
BsiHKAI GWGCWC 2 cut(s) 468, 1593
BsiHKCI CYCGRG 3 cut(s) 185, 467, 1580
BsiSI CCGG 7 cut(s) 7, 168, 224, 239, 294, 741, 1017
BslFI GGGAC 3 cut(s) 320, 735, 869
BslI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
BsmAI GTCTC 2 cut(s) 1139, 1164
BsmFI GGGAC 3 cut(s) 320, 735, 869
BsnI GGCC 9 cut(s) 75, 116, 160, 234, 552, 635, 750, 893, 1154
Bso31I GGTCTC 1 cut(s) 1164
BsoBI CYCGRG 3 cut(s) 185, 467, 1580
Bsp1286I GDGCHC 4 cut(s) 294, 468, 626, 1593
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
Bsp19I CCATGG 1 cut(s) 1524
BspANI GGCC 9 cut(s) 75, 116, 160, 234, 552, 635, 750, 893, 1154
BspCNI CTCAG 3 cut(s) 616, 756, 1125
BspFNI CGCG 2 cut(s) 638, 1157
BspLI GGNNCC 4 cut(s) 24, 336, 723, 1153
BspMAI CTGCAG 1 cut(s) 1395
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 3 cut(s) 56, 179, 235
BspQI GCTCTTC 3 cut(s) 467, 837, 1598
BspTNI GGTCTC 1 cut(s) 1164
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 1289
BsrFI RCCGGY 2 cut(s) 6, 1016
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 2 cut(s) 339, 350
BssAI RCCGGY 2 cut(s) 6, 1016
BssECI CCNNGG 3 cut(s) 132, 636, 1524
BssMI GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
BssNI GRCGYC 1 cut(s) 1224
BssT1I CCWWGG 1 cut(s) 1524
Bst2UI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
Bst4CI ACNGT 2 cut(s) 154, 860
Bst6I CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1598
BstACI GRCGYC 1 cut(s) 1224
BstAPI GCANNNNNTGC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 8 cut(s) 626, 682, 691, 748, 876, 1204, 1233, 1317
BstDEI CTNAG 4 cut(s) 603, 764, 888, 1133
BstDSI CCRYGG 2 cut(s) 636, 1524
BstF5I GGATG 4 cut(s) 520, 1261, 1327, 1563
BstFNI CGCG 2 cut(s) 638, 1157
BstH2I RGCGCY 1 cut(s) 1238
BstHHI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
BstKTI GATC 9 cut(s) 51, 174, 207, 230, 704, 1161, 1468, 1548, 1611
BstMAI GTCTC 2 cut(s) 1139, 1164
BstMBI GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
BstMCI CGRYCG 1 cut(s) 1021
BstNI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
BstNSI RCATGY 1 cut(s) 1319
BstSCI CCNGG 9 cut(s) 55, 132, 167, 223, 237, 292, 732, 1106, 1364
BstSFI CTRYAG 1 cut(s) 1391
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 2 cut(s) 638, 1157
BstXI CCANNNNNNTGG 1 cut(s) 112
BsuI GTATCC 1 cut(s) 1274
BsuRI GGCC 9 cut(s) 75, 116, 160, 234, 552, 635, 750, 893, 1154
BtgI CCRYGG 2 cut(s) 636, 1524
BtrI CACGTC 2 cut(s) 595, 754
BtsCI GGATG 4 cut(s) 520, 1261, 1327, 1563
BtsI GCAGTG 2 cut(s) 843, 1294
BtsIMutI CAGTG 4 cut(s) 346, 693, 843, 1294
Cac8I GCNNGC 8 cut(s) 626, 682, 691, 748, 876, 1204, 1233, 1317
CaiI CAGNNNCTG 2 cut(s) 345, 1358
CfoI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
Cfr10I RCCGGY 2 cut(s) 6, 1016
Cfr13I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
Cfr42I CCGCGG 1 cut(s) 639
CseI GACGC 1 cut(s) 1232
CsiI ACCWGGT 1 cut(s) 55
Csp6I GTAC 3 cut(s) 100, 958, 987
CspCI CAANNNNNGTGG 2 cut(s) 1163, 1198
CviQI GTAC 3 cut(s) 100, 958, 987
DdeI CTNAG 4 cut(s) 603, 764, 888, 1133
DpnI GATC 9 cut(s) 50, 173, 206, 229, 703, 1160, 1467, 1547, 1610
DpnII GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
DraI TTTAAA 1 cut(s) 1210
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1598
EarI CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1598
Ecl136II GAGCTC 1 cut(s) 1591
Eco130I CCWWGG 1 cut(s) 1524
Eco147I AGGCCT 1 cut(s) 160
Eco24I GRGCYC 2 cut(s) 626, 1593
Eco31I GGTCTC 1 cut(s) 1164
Eco32I GATATC 1 cut(s) 1003
Eco47I GGWCC 3 cut(s) 4, 334, 722
Eco47III AGCGCT 1 cut(s) 1236
Eco53kI GAGCTC 1 cut(s) 1591
Eco57I CTGAAG 3 cut(s) 103, 1380, 1482
Eco88I CYCGRG 3 cut(s) 185, 467, 1580
EcoICRI GAGCTC 1 cut(s) 1591
EcoO109I RGGNCCY 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
EcoRV GATATC 1 cut(s) 1003
EcoT14I CCWWGG 1 cut(s) 1524
EcoT38I GRGCYC 2 cut(s) 626, 1593
ErhI CCWWGG 1 cut(s) 1524
FaqI GGGAC 3 cut(s) 320, 735, 869
FauI CCCGC 2 cut(s) 633, 933
FbaI TGATCA 1 cut(s) 1545
FokI GGATG 4 cut(s) 527, 1268, 1334, 1570
FriOI GRGCYC 2 cut(s) 626, 1593
FspBI CTAG 2 cut(s) 833, 875
FspI TGCGCA 2 cut(s) 89, 1300
GlaI GCGC 5 cut(s) 89, 848, 1236, 1300, 1389
GsaI CCCAGC 1 cut(s) 1422
GsuI CTGGAG 2 cut(s) 1163, 1262
HaeII RGCGCY 1 cut(s) 1238
HaeIII GGCC 9 cut(s) 75, 116, 160, 234, 552, 635, 750, 893, 1154
HapII CCGG 7 cut(s) 7, 168, 224, 239, 294, 741, 1017
HgaI GACGC 1 cut(s) 1232
HhaI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
Hin1I GRCGYC 1 cut(s) 1224
Hin6I GCGC 5 cut(s) 88, 847, 1235, 1299, 1388
HinP1I GCGC 5 cut(s) 88, 847, 1235, 1299, 1388
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HindIII AAGCTT 1 cut(s) 16
HpaII CCGG 7 cut(s) 7, 168, 224, 239, 294, 741, 1017
HphI GGTGA 8 cut(s) 166, 206, 402, 662, 929, 1132, 1351, 1576
Hpy166II GTNNAC 5 cut(s) 102, 351, 410, 1185, 1222
Hpy188III TCNNGA 4 cut(s) 139, 187, 469, 578
Hpy8I GTNNAC 5 cut(s) 102, 351, 410, 1185, 1222
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 7 cut(s) 127, 227, 277, 492, 846, 1085, 1240
HpyCH4III ACNGT 2 cut(s) 154, 860
HpyCH4IV ACGT 3 cut(s) 93, 594, 753
HpyF3I CTNAG 4 cut(s) 603, 764, 888, 1133
HpySE526I ACGT 3 cut(s) 93, 594, 753
Hsp92I GRCGYC 1 cut(s) 1224
HspAI GCGC 5 cut(s) 88, 847, 1235, 1299, 1388
Ksp22I TGATCA 1 cut(s) 1545
KspI CCGCGG 1 cut(s) 639
Kzo9I GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
LguI GCTCTTC 3 cut(s) 467, 837, 1598
LmnI GCTCC 4 cut(s) 22, 296, 743, 1349
LweI GCATC 3 cut(s) 319, 787, 1345
MabI ACCWGGT 1 cut(s) 55
MaeI CTAG 2 cut(s) 833, 875
MaeII ACGT 3 cut(s) 93, 594, 753
MaeIII GTNAC 5 cut(s) 68, 341, 917, 1052, 1381
MalI GATC 9 cut(s) 50, 173, 206, 229, 703, 1160, 1467, 1547, 1610
MbiI CCGCTC 1 cut(s) 184
MboI GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
MhlI GDGCHC 4 cut(s) 294, 468, 626, 1593
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 5 cut(s) 31, 581, 777, 1128, 1645
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 7 cut(s) 236, 257, 494, 716, 1064, 1412, 1572
Mox20I TGGCCA 1 cut(s) 750
MscI TGGCCA 1 cut(s) 750
MseI TTAA 3 cut(s) 780, 1209, 1635
MslI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 5 cut(s) 384, 571, 638, 928, 1304
MspI CCGG 7 cut(s) 7, 168, 224, 239, 294, 741, 1017
MspR9I CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
MvaI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
MvnI CGCG 2 cut(s) 638, 1157
NciI CCSGG 4 cut(s) 169, 225, 239, 294
NcoI CCATGG 1 cut(s) 1524
NdeII GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
NheI GCTAGC 1 cut(s) 874
NlaIV GGNNCC 4 cut(s) 24, 336, 723, 1153
NmeAIII GCCGAG 1 cut(s) 1426
NmuCI GTSAC 4 cut(s) 341, 917, 1052, 1381
NsbI TGCGCA 2 cut(s) 89, 1300
NspI RCATGY 1 cut(s) 1319
PaeI GCATGC 1 cut(s) 1319
PaeR7I CTCGAG 3 cut(s) 185, 467, 1580
PceI AGGCCT 1 cut(s) 160
PciSI GCTCTTC 3 cut(s) 467, 837, 1598
PcsI WCGNNNNNNNCGW 3 cut(s) 474, 591, 1582
PfeI GAWTC 5 cut(s) 142, 509, 1307, 1432, 1483
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 2 cut(s) 167, 237
PleI GAGTC 7 cut(s) 236, 257, 494, 715, 1064, 1411, 1572
PpsI GAGTC 7 cut(s) 236, 257, 494, 715, 1064, 1411, 1572
PpuMI RGGWCCY 1 cut(s) 722
PsiI TTATAA 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 1593
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
PspFI CCCAGC 1 cut(s) 1418
PspGI CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
PspN4I GGNNCC 4 cut(s) 24, 336, 723, 1153
PspPI GGNCC 5 cut(s) 4, 232, 334, 722, 1152
PspPPI RGGWCCY 1 cut(s) 722
PspXI VCTCGAGB 1 cut(s) 1580
PstI CTGCAG 1 cut(s) 1395
PstNI CAGNNNCTG 2 cut(s) 345, 1358
PsyI GACNNNGTC 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 3 cut(s) 101, 959, 988
RsaNI GTAC 3 cut(s) 100, 958, 987
RseI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SacI GAGCTC 1 cut(s) 1593
SacII CCGCGG 1 cut(s) 639
SapI GCTCTTC 3 cut(s) 467, 837, 1598
SaqAI TTAA 3 cut(s) 780, 1209, 1635
Sau3AI GATC 9 cut(s) 48, 171, 204, 227, 701, 1158, 1465, 1545, 1608
Sau96I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
SchI GAGTC 7 cut(s) 236, 257, 494, 716, 1064, 1412, 1572
ScrFI CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
SduI GDGCHC 4 cut(s) 294, 468, 626, 1593
SexAI ACCWGGT 1 cut(s) 55
SfaNI GCATC 3 cut(s) 319, 787, 1345
SfcI CTRYAG 1 cut(s) 1391
Sfr274I CTCGAG 3 cut(s) 185, 467, 1580
Sfr303I CCGCGG 1 cut(s) 639
SgrBI CCGCGG 1 cut(s) 639
SinI GGWCC 3 cut(s) 4, 334, 722
SlaI CTCGAG 3 cut(s) 185, 467, 1580
SmiMI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SmlI CTYRAG 5 cut(s) 185, 467, 546, 629, 1580
SmoI CTYRAG 5 cut(s) 185, 467, 546, 629, 1580
SphI GCATGC 1 cut(s) 1319
Sse9I AATT 5 cut(s) 31, 581, 777, 1128, 1645
SseBI AGGCCT 1 cut(s) 160
SspI AATATT 1 cut(s) 1639
SspMI CTAG 2 cut(s) 833, 875
SstI GAGCTC 1 cut(s) 1593
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 9 cut(s) 55, 132, 167, 223, 237, 292, 732, 1106, 1364
StyI CCWWGG 1 cut(s) 1524
TaaI ACNGT 2 cut(s) 154, 860
TaiI ACGT 3 cut(s) 96, 597, 756
TasI AATT 5 cut(s) 31, 581, 777, 1128, 1645
TatI WGTACW 2 cut(s) 99, 957
TauI GCSGC 5 cut(s) 638, 641, 941, 1043, 1157
TfiI GAWTC 5 cut(s) 142, 509, 1307, 1432, 1483
Tru1I TTAA 3 cut(s) 780, 1209, 1635
Tru9I TTAA 3 cut(s) 780, 1209, 1635
TscAI CASTG 4 cut(s) 346, 700, 843, 1301
TseFI GTSAC 4 cut(s) 341, 917, 1052, 1381
Tsp45I GTSAC 4 cut(s) 341, 917, 1052, 1381
TspDTI ATGAA 5 cut(s) 306, 364, 391, 474, 1493
TspGWI ACGGA 4 cut(s) 61, 828, 1179, 1293
TspRI CASTG 4 cut(s) 346, 700, 843, 1301
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 4, 334, 722
XapI RAATTY 2 cut(s) 31, 581
XceI RCATGY 1 cut(s) 1319
XhoI CTCGAG 3 cut(s) 185, 467, 1580
XspI CTAG 2 cut(s) 833, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.