MD12G1008200.v1.1

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013599
Physical Location & Seq
Reverse (-)
883944 .. 885569
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1008200.v1.1.491

Sequence Viewer

Length: 1626 bp
ATGGACAGGCTCATAAGTTTGGAGCCATCAAACCTTGTTGCAATACGGATCGAACCGGGACAGAAATGCTACGGCCAGCTCACTTTACGCAACGTTATGTACACCATGCCGGTAGCCTTCCGGCTCGAAGCGTTGATCAAGAATCGTTACACGGTGAGGCCTCAGTCCGGGATCATATCTCCTCTCGAGAAGTTGACGGTAGAGATTGTTTATCATCTTCCGCCCGGGTCCAGCCTCCCGGACTCCTTTCCTTACTGCCATGATTCCTTCCTTTTGCATAGCGTGGTTGTGCCTGGAGCGGCGATCAAAGTGTCTTCTTCGACATTCGATGCGGTTCCAAACGATTGGTTCACCACAAAGAAGAAACAAGTGTTCATAGATAGTGGTGTCAAGATTATGTTTGTTGGTTCACCTATTTTGGCACAACTTGTGTCCGATGGTTTGATGGATGATATTCGAGAAGTGCTTGAGAAGAGTGAACCTTCATGGAGAGCCGCCGATTCTGTGGATTCTGAGGGCCAATCTTTGATTCACTTGGCAGTTGCTCAAGGCCGGCCTGACCTTGTCCAGCTACTTCTTGAATTTGAGCCCGATGTGGAGGCTCAAAGCCAGTCAGGATCTACTCCGCTTGAGGTTGCAGCCTCAAGAGGAGAAGCCTTGATAGTTGAGCTTCTATTGGCTCGACGTGCCAGCACTGAACGATCTGAGTCCTCCACTTGGGGACCTATTCACCTCGCGGTCGGAGGAGGCCACGTAGAGGTTCTTAGGCTTCTTATAATCAAAGGGGCAAATGTTGATGCGCTGACAAAGGATGGTAGCACGGCTTTGCACCTTGCTGTTGAGGAGCGAAGAAGGGACAGTGCGAGGCTCTTATTGGCAAGCGGGGCTAAAGCTGAAGTTAGTGATTGTAGAGATGGTGACACGCCTTTGCAAATCGCGGCAAGCCTAGGGGACGAGTACATGGTGAAGCTGTTGCTGCAAAAGGGTGCGAATAAAGATGTAAGAAACTTTGCCGGTCTAACAGCCTATGATGTTGCGGCGGAGAATGGACACACGAGACTATTTGATGCCCTTCGACTTGGTGATAGCTTGTGTATCGCGGCGAGGAAGGGAGAGGTAAGAACAGTTGTAAAGTTGCTTGAGACAGGTGCGGCAATCAATGGAAGAGACCTACATGGGTGGACTGCTCTCCATAGAGCCTGTTTCAAAGGGAAGATAGAAGTCATTCGAACGCTGCTTGAAAAGGGTGTGGATATGGACGCTAAAGATGAGGATGGATACACTGCGTTGCACTGTGCAGCGGAGTCGGGACATGCGGATGTGATTGAGATGTTGGTAAAGAAAGGAGCTGATGCTGAAGCTCGAACTAACAAAGGTGTGACTGCACTGCAGATTGCTGAGTCTTTGCACTACGTTGGGATTACTAGGATACTTATTCATAGAGGTGCAACAAAAGACAATAGTAATATGGCACATATCTTGACCCAGGCCTCAGTTTCATTTGGCAAAAAATCTATGGGACTAGAGGAGGAGATCATCAAAGGAGGAATGAAGAAGAAGAAATCGAGTCGAGCTAGAGCTCTTCATGGAAGCTTCAATCGTTCGATGCCATTAGCTGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

542

Amino Acids

58.63

Weight (kDa)

6.78

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 5 - 105 6.3e-07 MSP (Major sperm protein) domain
Ank_2 PF12796 166 - 230 4.4e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 168 - 213 8.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 239 - 301 4.1e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 243 - 292 2.9e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 297 - 358 1.1e-07 Ankyrin repeats (3 copies)
Ank PF00023 305 - 336 7e-07 Ankyrin repeat
Ank_2 PF12796 364 - 423 3.9e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 364 - 412 3.4e-07 Ankyrin repeats (many copies)
Ank PF00023 392 - 423 4.6e-07 Ankyrin repeat
Ank_4 PF13637 399 - 446 1.2e-12 Ankyrin repeats (many copies)
Ank_5 PF13857 412 - 466 5e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 417 - 486 7.7e-12 Ankyrin repeats (3 copies)
Ank PF00023 425 - 457 1.9e-08 Ankyrin repeat
Ank_3 PF13606 425 - 453 6.4e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
AccBSI CCGCTC 1 cut(s) 299
AccII CGCG 3 cut(s) 737, 938, 1100
AclI AACGTT 1 cut(s) 93
AclWI GGATC 3 cut(s) 56, 179, 625
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 2 cut(s) 915, 1377
AfaI GTAC 2 cut(s) 101, 959
AfiI CCNNNNNNNGG 3 cut(s) 167, 717, 757
AgsI TTSAA 4 cut(s) 581, 1207, 1241, 1597
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 2 cut(s) 292, 1485
AjuI GAANNNNNNNTTGG 2 cut(s) 513, 545
Alw21I GWGCWC 1 cut(s) 1582
Alw26I GTCTC 3 cut(s) 1051, 1136, 1161
AlwI GGATC 3 cut(s) 56, 179, 625
Ama87I CYCGRG 2 cut(s) 185, 224
AoxI GGCC 7 cut(s) 73, 158, 517, 550, 554, 748, 1488
ApeKI GCWGC 4 cut(s) 638, 976, 1234, 1298
ApoI RAATTY 1 cut(s) 581
Asp700I GAANNNNTTC 1 cut(s) 1224
AspA2I CCTAGG 1 cut(s) 946
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 228, 517, 722
AsuC2I CCSGG 5 cut(s) 57, 169, 225, 226, 239
AsuHPI GGTGA 7 cut(s) 166, 343, 402, 722, 929, 976, 1094
AsuII TTCGAA 1 cut(s) 1228
AvaI CYCGRG 2 cut(s) 185, 224
AvaII GGWCC 2 cut(s) 228, 722
AvrII CCTAGG 1 cut(s) 946
BanII GRGCYC 2 cut(s) 591, 1582
BauI CACGAG 1 cut(s) 1054
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 1582
BbvI GCAGC 4 cut(s) 650, 963, 1221, 1310
BccI CCATC 6 cut(s) 34, 431, 439, 806, 908, 1268
BceAI ACGGC 2 cut(s) 88, 837
BcgI CGANNNNNNTGC 2 cut(s) 1287, 1321
BciT130I CCWGG 2 cut(s) 294, 1487
BciVI GTATCC 2 cut(s) 1271, 1422
BclI TGATCA 1 cut(s) 135
BcnI CCSGG 5 cut(s) 57, 169, 225, 226, 239
BcoDI GTCTC 3 cut(s) 1051, 1136, 1161
BfaI CTAG 4 cut(s) 947, 1425, 1523, 1575
BfmI CTRYAG 1 cut(s) 1388
BfuI GTATCC 2 cut(s) 1271, 1422
BlnI CCTAGG 1 cut(s) 946
Bme1390I CCNGG 7 cut(s) 57, 169, 225, 226, 239, 294, 1487
Bme18I GGWCC 2 cut(s) 228, 722
BmeT110I CYCGRG 2 cut(s) 185, 224
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 3 cut(s) 228, 517, 722
BmiI GGNNCC 4 cut(s) 24, 229, 336, 723
BmrFI CCNGG 7 cut(s) 57, 169, 225, 226, 239, 294, 1487
BmsI GCATC 5 cut(s) 319, 787, 1057, 1342, 1596
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 315
Bpu14I TTCGAA 1 cut(s) 1228
BpuEI CTTGAG 5 cut(s) 488, 531, 628, 650, 1160
BpuMI CCSGG 5 cut(s) 57, 169, 225, 226, 239
BsaAI YACGTR 1 cut(s) 754
BsaI GGTCTC 1 cut(s) 1161
BsaJI CCNNGG 3 cut(s) 224, 946, 1485
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 717, 757
Bse118I RCCGGY 3 cut(s) 109, 552, 1013
Bse1I ACTGG 1 cut(s) 610
BseBI CCWGG 2 cut(s) 294, 1487
BseDI CCNNGG 3 cut(s) 224, 946, 1485
BseGI GGATG 4 cut(s) 454, 817, 1279, 1324
BseLI CCNNNNNNNGG 3 cut(s) 167, 717, 757
BseMII CTCAG 5 cut(s) 176, 504, 696, 1389, 1506
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 6 cut(s) 171, 663, 759, 857, 1541, 1544
BseXI GCAGC 4 cut(s) 650, 963, 1221, 1310
BsgI GTGCAG 2 cut(s) 1317, 1368
Bsh1236I CGCG 3 cut(s) 737, 938, 1100
Bsh1285I CGRYCG 1 cut(s) 741
BshFI GGCC 7 cut(s) 75, 160, 519, 552, 556, 750, 1490
BsiEI CGRYCG 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 1582
BsiHKCI CYCGRG 2 cut(s) 185, 224
BsiSI CCGG 8 cut(s) 56, 110, 121, 168, 225, 239, 553, 1014
BslFI GGGAC 6 cut(s) 72, 735, 869, 965, 1323, 1533
BslI CCNNNNNNNGG 3 cut(s) 167, 717, 757
BsmAI GTCTC 3 cut(s) 1051, 1136, 1161
BsmFI GGGAC 6 cut(s) 72, 735, 869, 965, 1323, 1533
BsnI GGCC 7 cut(s) 75, 160, 519, 552, 556, 750, 1490
Bso31I GGTCTC 1 cut(s) 1161
BsoBI CYCGRG 2 cut(s) 185, 224
Bsp119I TTCGAA 1 cut(s) 1228
Bsp1286I GDGCHC 2 cut(s) 591, 1582
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
BspANI GGCC 7 cut(s) 75, 160, 519, 552, 556, 750, 1490
BspCNI CTCAG 5 cut(s) 175, 505, 697, 1390, 1505
BspFNI CGCG 3 cut(s) 737, 938, 1100
BspLI GGNNCC 4 cut(s) 24, 229, 336, 723
BspMAI CTGCAG 1 cut(s) 1392
BspPI GGATC 3 cut(s) 56, 179, 625
BspQI GCTCTTC 1 cut(s) 1587
BspT104I TTCGAA 1 cut(s) 1228
BspTNI GGTCTC 1 cut(s) 1161
BsrBI CCGCTC 1 cut(s) 299
BsrFI RCCGGY 3 cut(s) 109, 552, 1013
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 3 cut(s) 109, 552, 1013
BssECI CCNNGG 3 cut(s) 224, 946, 1485
BssMI GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
BssSI CACGAG 1 cut(s) 1054
BssT1I CCWWGG 1 cut(s) 946
Bst2BI CACGAG 1 cut(s) 1054
Bst2UI CCWGG 2 cut(s) 294, 1487
Bst4CI ACNGT 5 cut(s) 154, 199, 860, 1126, 1295
Bst6I CTCTTC 3 cut(s) 467, 1159, 1587
BstAUI TGTACA 1 cut(s) 99
BstBAI YACGTR 1 cut(s) 754
BstBI TTCGAA 1 cut(s) 1228
BstC8I GCNNGC 5 cut(s) 77, 554, 691, 880, 943
BstDEI CTNAG 6 cut(s) 162, 513, 705, 764, 1398, 1492
BstF5I GGATG 4 cut(s) 454, 817, 1279, 1324
BstFNI CGCG 3 cut(s) 737, 938, 1100
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 7 cut(s) 51, 138, 174, 306, 620, 704, 1536
BstMAI GTCTC 3 cut(s) 1051, 1136, 1161
BstMBI GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
BstMCI CGRYCG 1 cut(s) 741
BstMWI GCNNNNNNNGC 3 cut(s) 686, 884, 976
BstNI CCWGG 2 cut(s) 294, 1487
BstNSI RCATGY 1 cut(s) 1316
BstSCI CCNGG 7 cut(s) 55, 167, 223, 224, 237, 292, 1485
BstSFI CTRYAG 1 cut(s) 1388
BstUI CGCG 3 cut(s) 737, 938, 1100
BstV1I GCAGC 4 cut(s) 650, 963, 1221, 1310
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 617
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 1 cut(s) 617
BsuI GTATCC 2 cut(s) 1271, 1422
BsuRI GGCC 7 cut(s) 75, 160, 519, 552, 556, 750, 1490
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 4 cut(s) 454, 817, 1279, 1324
BtsI GCAGTG 2 cut(s) 1281, 1385
BtsIMutI CAGTG 5 cut(s) 693, 865, 1281, 1291, 1385
Cac8I GCNNGC 5 cut(s) 77, 554, 691, 880, 943
CfoI GCGC 1 cut(s) 802
Cfr10I RCCGGY 3 cut(s) 109, 552, 1013
Cfr13I GGNCC 3 cut(s) 228, 517, 722
Cfr9I CCCGGG 1 cut(s) 224
CseI GACGC 1 cut(s) 1268
Csp6I GTAC 2 cut(s) 100, 958
CviAII CATG 7 cut(s) 106, 260, 486, 961, 1175, 1313, 1586
CviQI GTAC 2 cut(s) 100, 958
DdeI CTNAG 6 cut(s) 162, 513, 705, 764, 1398, 1492
DpnI GATC 7 cut(s) 50, 137, 173, 305, 619, 703, 1535
DpnII GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 3 cut(s) 467, 1159, 1587
EarI CTCTTC 3 cut(s) 467, 1159, 1587
EciI GGCGGA 2 cut(s) 210, 1055
Ecl136II GAGCTC 1 cut(s) 1580
Eco130I CCWWGG 1 cut(s) 946
Eco147I AGGCCT 2 cut(s) 160, 1490
Eco24I GRGCYC 2 cut(s) 591, 1582
Eco31I GGTCTC 1 cut(s) 1161
Eco47I GGWCC 2 cut(s) 228, 722
Eco53kI GAGCTC 1 cut(s) 1580
Eco57I CTGAAG 2 cut(s) 915, 1377
Eco88I CYCGRG 2 cut(s) 185, 224
EcoICRI GAGCTC 1 cut(s) 1580
EcoO109I RGGNCCY 1 cut(s) 722
EcoRII CCWGG 2 cut(s) 292, 1485
EcoT14I CCWWGG 1 cut(s) 946
EcoT38I GRGCYC 2 cut(s) 591, 1582
ErhI CCWWGG 1 cut(s) 946
FaeI CATG 7 cut(s) 109, 263, 489, 964, 1178, 1316, 1589
FaqI GGGAC 6 cut(s) 72, 735, 869, 965, 1323, 1533
FatI CATG 7 cut(s) 105, 259, 485, 960, 1174, 1312, 1585
FauI CCCGC 1 cut(s) 875
FbaI TGATCA 1 cut(s) 135
FokI GGATG 4 cut(s) 461, 824, 1286, 1331
FriOI GRGCYC 2 cut(s) 591, 1582
FseI GGCCGGCC 1 cut(s) 556
FspBI CTAG 4 cut(s) 947, 1425, 1523, 1575
GlaI GCGC 1 cut(s) 801
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 7 cut(s) 75, 160, 519, 552, 556, 750, 1490
HapII CCGG 8 cut(s) 56, 110, 121, 168, 225, 239, 553, 1014
HgaI GACGC 1 cut(s) 1268
HhaI GCGC 1 cut(s) 802
Hin1II CATG 7 cut(s) 109, 263, 489, 964, 1178, 1316, 1589
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HindIII AAGCTT 1 cut(s) 1591
HpaII CCGG 8 cut(s) 56, 110, 121, 168, 225, 239, 553, 1014
HphI GGTGA 7 cut(s) 166, 343, 402, 722, 929, 976, 1094
Hpy166II GTNNAC 6 cut(s) 102, 195, 351, 410, 479, 1182
Hpy188I TCNGA 4 cut(s) 436, 514, 706, 743
Hpy8I GTNNAC 6 cut(s) 102, 195, 351, 410, 479, 1182
Hpy99I CGWCG 1 cut(s) 687
HpyAV CCTTC 6 cut(s) 127, 277, 492, 846, 1082, 1102
HpyCH4III ACNGT 5 cut(s) 154, 199, 860, 1126, 1295
HpyCH4IV ACGT 4 cut(s) 93, 685, 753, 1413
HpyF10VI GCNNNNNNNGC 3 cut(s) 686, 884, 976
HpyF3I CTNAG 6 cut(s) 162, 513, 705, 764, 1398, 1492
HpySE526I ACGT 4 cut(s) 93, 685, 753, 1413
Hsp92II CATG 7 cut(s) 109, 263, 489, 964, 1178, 1316, 1589
HspAI GCGC 1 cut(s) 800
KroI GCCGGC 1 cut(s) 552
KroNI GCCGGC 1 cut(s) 554
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
LguI GCTCTTC 1 cut(s) 1587
LmnI GCTCC 4 cut(s) 22, 296, 844, 1346
Lsp1109I GCAGC 4 cut(s) 650, 963, 1221, 1310
LweI GCATC 5 cut(s) 319, 787, 1057, 1342, 1596
MaeI CTAG 4 cut(s) 947, 1425, 1523, 1575
MaeII ACGT 4 cut(s) 93, 685, 753, 1413
MaeIII GTNAC 3 cut(s) 146, 917, 1378
MalI GATC 7 cut(s) 50, 137, 173, 305, 619, 703, 1535
MbiI CCGCTC 1 cut(s) 299
MboI GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 2 cut(s) 591, 1582
MluCI AATT 1 cut(s) 581
MlyI GAGTC 5 cut(s) 236, 716, 1313, 1409, 1576
MmeI TCCRAC 1 cut(s) 721
MroNI GCCGGC 1 cut(s) 552
MroXI GAANNNNTTC 1 cut(s) 1224
MslI CAYNNNNRTG 2 cut(s) 1317, 1443
MspA1I CMGCKG 1 cut(s) 1301
MspI CCGG 8 cut(s) 56, 110, 121, 168, 225, 239, 553, 1014
MspR9I CCNGG 7 cut(s) 57, 169, 225, 226, 239, 294, 1487
MvaI CCWGG 2 cut(s) 294, 1487
MvnI CGCG 3 cut(s) 737, 938, 1100
MwoI GCNNNNNNNGC 3 cut(s) 686, 884, 976
NaeI GCCGGC 1 cut(s) 554
NciI CCSGG 5 cut(s) 57, 169, 225, 226, 239
NdeII GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
NgoMIV GCCGGC 1 cut(s) 552
NlaIII CATG 7 cut(s) 109, 263, 489, 964, 1178, 1316, 1589
NlaIV GGNNCC 4 cut(s) 24, 229, 336, 723
NmuCI GTSAC 2 cut(s) 917, 1378
NspI RCATGY 1 cut(s) 1316
NspV TTCGAA 1 cut(s) 1228
PaeR7I CTCGAG 1 cut(s) 185
PceI AGGCCT 2 cut(s) 160, 1490
PciSI GCTCTTC 1 cut(s) 1587
PdiI GCCGGC 1 cut(s) 554
PdmI GAANNNNTTC 1 cut(s) 1224
PfeI GAWTC 5 cut(s) 142, 263, 500, 509, 529
PflFI GACNNNGTC 1 cut(s) 563
PfoI TCCNGGA 2 cut(s) 167, 237
PleI GAGTC 5 cut(s) 236, 715, 1312, 1408, 1575
PpsI GAGTC 5 cut(s) 236, 715, 1312, 1408, 1575
Ppu21I YACGTR 1 cut(s) 754
PpuMI RGGWCCY 1 cut(s) 722
PsiI TTATAA 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 1582
Psp1406I AACGTT 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 292, 1485
PspGI CCWGG 2 cut(s) 292, 1485
PspN4I GGNNCC 4 cut(s) 24, 229, 336, 723
PspPI GGNCC 3 cut(s) 228, 517, 722
PspPPI RGGWCCY 1 cut(s) 722
PstI CTGCAG 1 cut(s) 1392
PsuI RGATCY 1 cut(s) 617
PsyI GACNNNGTC 1 cut(s) 563
RigI GGCCGGCC 1 cut(s) 556
RsaI GTAC 2 cut(s) 101, 959
RsaNI GTAC 2 cut(s) 100, 958
RseI CAYNNNNRTG 2 cut(s) 1317, 1443
SacI GAGCTC 1 cut(s) 1582
SapI GCTCTTC 1 cut(s) 1587
Sau3AI GATC 7 cut(s) 48, 135, 171, 303, 617, 701, 1533
Sau96I GGNCC 3 cut(s) 228, 517, 722
SchI GAGTC 5 cut(s) 236, 716, 1313, 1409, 1576
ScrFI CCNGG 7 cut(s) 57, 169, 225, 226, 239, 294, 1487
SduI GDGCHC 2 cut(s) 591, 1582
SfaNI GCATC 5 cut(s) 319, 787, 1057, 1342, 1596
SfcI CTRYAG 1 cut(s) 1388
Sfr274I CTCGAG 1 cut(s) 185
SfuI TTCGAA 1 cut(s) 1228
SinI GGWCC 2 cut(s) 228, 722
SlaI CTCGAG 1 cut(s) 185
SmaI CCCGGG 1 cut(s) 226
SmiMI CAYNNNNRTG 2 cut(s) 1317, 1443
SmlI CTYRAG 6 cut(s) 185, 467, 546, 629, 643, 1139
SmoI CTYRAG 6 cut(s) 185, 467, 546, 629, 643, 1139
Sse9I AATT 1 cut(s) 581
SseBI AGGCCT 2 cut(s) 160, 1490
SspMI CTAG 4 cut(s) 947, 1425, 1523, 1575
SstI GAGCTC 1 cut(s) 1582
StuI AGGCCT 2 cut(s) 160, 1490
StyD4I CCNGG 7 cut(s) 55, 167, 223, 224, 237, 292, 1485
StyI CCWWGG 1 cut(s) 946
TaaI ACNGT 5 cut(s) 154, 199, 860, 1126, 1295
TaiI ACGT 4 cut(s) 96, 688, 756, 1416
TasI AATT 1 cut(s) 581
TatI WGTACW 2 cut(s) 99, 957
TauI GCSGC 6 cut(s) 302, 497, 941, 1040, 1103, 1154
TfiI GAWTC 5 cut(s) 142, 263, 500, 509, 529
TscAI CASTG 5 cut(s) 700, 865, 1288, 1298, 1392
TseFI GTSAC 2 cut(s) 917, 1378
TseI GCWGC 4 cut(s) 638, 976, 1234, 1298
Tsp45I GTSAC 2 cut(s) 917, 1378
TspDTI ATGAA 6 cut(s) 364, 474, 1427, 1488, 1565, 1574
TspGWI ACGGA 1 cut(s) 61
TspMI CCCGGG 1 cut(s) 224
TspRI CASTG 5 cut(s) 700, 865, 1288, 1298, 1392
Tth111I GACNNNGTC 1 cut(s) 563
VpaK11BI GGWCC 2 cut(s) 228, 722
XapI RAATTY 1 cut(s) 581
XceI RCATGY 1 cut(s) 1316
XhoI CTCGAG 1 cut(s) 185
XmaI CCCGGG 1 cut(s) 224
XmaJI CCTAGG 1 cut(s) 946
XmnI GAANNNNTTC 1 cut(s) 1224
XspI CTAG 4 cut(s) 947, 1425, 1523, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.