RLG00000022523

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013599
Physical Location & Seq
Reverse (-)
2756177 .. 2758900
2724 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022523

Sequence Viewer

Length: 1731 bp
ATGGACCGGCTCATAAGCTTGGAGCCACCAAATTTCGTTGCAATACGGATTGAACCAGGTCAGAAATGTTACGGCCTACTCACTTTGCGCAACGTCATGTACACCATGCCTGTGGCCTTCAGGCTTCAAGCCCTGGTCAAGAATCGCTACACGGTGAGGCCTCAATCCGGGATCATATCTCCACTCGAGAAGCTAACCATTGAGATCGTCTATCACCTTCCACCGGGATCGGGCCTCCCGGACTCGTTCCCTTATTGCCATGACTCCTTCCTTTTGCATAGCGTGGTCGTGCCCGGAGCAGCCATCAAAAACTCTTCATCGACTTTCGATGCGGTCCCCAGTGACTGGTTCACAACAAAGAAGAAGCAAGTGTTCATAGACAGCGGTGTGAAGATTATGTTCACTGGTTCACCTGTTTTGGCACAACTTGTGTCCGATGGTTTGATGGACGATATCAGAGAAGTGCTCGAGAAGAGCGACCCTTCATGGAAAGCAGCCGACTCTGTGGATTCGGATGGTCAATCTTTACTCCACTTGGCTATTGCTCAAGGCCGACCCGACCTCGTCCAGCTGCTTCTAGAATTCGAGCCAGACGTGGAGGCTCAGAGCCGTTCGGGGTCGAGCCCGCTTGAGGCCGCGGCTGCTTCGGGTGAGGCTTTGATAGTCGAGCTTCTGTTGGCTCGCCGTGCAAGCACTGAAAGATCAGAGTCCTCCACTTGGGGACCTATCCACCTGGCAGCCGGAGCTGGCCACGTCGAAGTTCTAAGGCTTCTTATAATTAAGGGGGCTAATGTTGATGCTCAAACAAAAGACGGAAACACCGCCTTACACCTAGCAGTGGAAGAGCGCAGAAGGGACTGTGCGAGGCTTTTGCTAGCAAGTGGTGCTAAGGCCGAAGTTCGTAATACCAAGGAGGGTGACACCCCGCTGCACATAGCCGCAGGGTTGGGAGATGAGTACATGGTCAAGCTGTTGCTGCAAAAGGGTACTGCCAATAAAGATATCAGAAACTACGCCGGTCGTACGGCTTATGATGTTGCCGCAGAGAATGGTCACACCAAGCTCTTTGACTCCCTTCGGCTCGGAGATAGCTTGTGTATAGCGGCCAGGAAAGGAGAGGTGAGAACAATTCTGAGGGTGCTGGAGACAGGGGCCGCGATCAACGGAAGAGACCAACATGGGTGGACTGCTCTGCATAGGGCGTGCTTTAAAGGCAAAGTTGACGCCGTGCGAGCGCTGCTGGAGAAGGGTGTGGATGTGGGTGCTAAAGATGAGGACGGATACACGGCATTGCACTGCGCAGCGGAATCGGGGCATGCGGATGTGATAGATATGTTGGTGAAGAAAGGAGCTGATGCTGAAGCCAGGACTAATAAGGGTGTGACTGCGCTGCAGATTGCCGAGTCTTTGCACTATGCTGGGATTGCAAGGATTCTTATGACACATGGAGGTGGTGCAATAAAAGATCAGAACATGGCACAGATTCTGTCTCACGCTTCAGTTCATTCGTTTGGGAAGAAATACATGGGCGTTGAGGAAGAGATGATCAAGGGAGGGATGATGGTATTGAAACTAACTACAGTTGATCCGGACGTAGGCATCAGACTTCTGGGTGTAGAAGACGATGATCAAGAGGATAACGAGACCGACGAGATGTATGCGACGGAGAATATTCCGAACATGTCATCGAAGGTGGTGATGGGTTGTTGGGCATTGCTCATAATATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

577

Amino Acids

62.28

Weight (kDa)

5.69

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 5 - 107 1.2e-07 MSP (Major sperm protein) domain
Ank_2 PF12796 164 - 230 4.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 239 - 301 6.9e-13 Ankyrin repeats (3 copies)
Ank PF00023 242 - 270 3.2e-06 Ankyrin repeat
Ank_4 PF13637 243 - 292 2.8e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 263 - 313 5.7e-06 Ankyrin repeats (many copies)
Ank PF00023 271 - 302 9.6e-06 Ankyrin repeat
Ank_2 PF12796 297 - 359 5.1e-09 Ankyrin repeats (3 copies)
Ank PF00023 306 - 337 2.6e-07 Ankyrin repeat
Ank_2 PF12796 365 - 424 1e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 381 - 432 3.3e-08 Ankyrin repeats (many copies)
Ank PF00023 393 - 424 5.7e-07 Ankyrin repeat
Ank_4 PF13637 400 - 447 7.9e-12 Ankyrin repeats (many copies)
Ank_5 PF13857 413 - 467 8.1e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 420 - 483 3.5e-10 Ankyrin repeats (3 copies)
Ank PF00023 426 - 458 5.9e-08 Ankyrin repeat
Ank_3 PF13606 426 - 454 7.7e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
Acc16I TGCGCA 2 cut(s) 89, 1300
AccB7I CCANNNNNTGG 1 cut(s) 345
AccII CGCG 2 cut(s) 638, 1157
AccIII TCCGGA 1 cut(s) 1588
AclWI GGATC 3 cut(s) 179, 235, 1580
AcoI YGGCCR 2 cut(s) 748, 1104
AcsI RAATTY 2 cut(s) 31, 581
AcuI CTGAAG 3 cut(s) 103, 1380, 1482
AcyI GRCGYC 1 cut(s) 1224
AfaI GTAC 4 cut(s) 101, 959, 988, 1024
AfeI AGCGCT 1 cut(s) 1236
AfiI CCNNNNNNNGG 8 cut(s) 167, 223, 230, 345, 631, 717, 838, 1595
AflIII ACRYGT 1 cut(s) 1680
AgsI TTSAA 3 cut(s) 53, 128, 1570
AjiI CACGTC 2 cut(s) 595, 754
AjnI CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
AluBI AGCT 9 cut(s) 18, 193, 571, 670, 746, 970, 1063, 1092, 1352
AluI AGCT 9 cut(s) 18, 193, 571, 670, 746, 970, 1063, 1092, 1352
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 4 cut(s) 1139, 1164, 1494, 1637
AlwI GGATC 3 cut(s) 179, 235, 1580
AlwNI CAGNNNCTG 2 cut(s) 345, 1486
Ama87I CYCGRG 2 cut(s) 185, 467
Aor13HI TCCGGA 1 cut(s) 1588
Aor51HI AGCGCT 1 cut(s) 1236
ApoI RAATTY 2 cut(s) 31, 581
AspLEI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
AspS9I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
AsuC2I CCSGG 4 cut(s) 169, 225, 239, 294
AsuHPI GGTGA 8 cut(s) 166, 206, 402, 662, 929, 1132, 1351, 1708
AsuNHI GCTAGC 1 cut(s) 874
AvaI CYCGRG 2 cut(s) 185, 467
AvaII GGWCC 3 cut(s) 4, 334, 722
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 750
BanII GRGCYC 1 cut(s) 626
BbsI GAAGAC 1 cut(s) 1626
Bbv12I GWGCWC 1 cut(s) 468
BccI CCATC 6 cut(s) 311, 431, 439, 509, 1555, 1693
BceAI ACGGC 6 cut(s) 88, 594, 669, 1041, 1211, 1302
BcgI CGANNNNNNTGC 6 cut(s) 853, 887, 1290, 1324, 1640, 1674
BciT130I CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
BciVI GTATCC 1 cut(s) 1274
BclI TGATCA 2 cut(s) 1545, 1627
BcnI CCSGG 4 cut(s) 169, 225, 239, 294
BcoDI GTCTC 4 cut(s) 1139, 1164, 1494, 1637
BfaI CTAG 3 cut(s) 578, 833, 875
BfmI CTRYAG 2 cut(s) 1391, 1578
BfoI RGCGCY 1 cut(s) 1238
BfuI GTATCC 1 cut(s) 1274
Bme1390I CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
Bme18I GGWCC 3 cut(s) 4, 334, 722
BmeT110I CYCGRG 2 cut(s) 185, 467
BmgBI CACGTC 2 cut(s) 595, 754
BmgT120I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
BmiI GGNNCC 4 cut(s) 24, 336, 723, 1153
BmrFI CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 4 cut(s) 319, 787, 1345, 1608
BmtI GCTAGC 1 cut(s) 878
BmuI ACTGGG 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 1626
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpmI CTGGAG 2 cut(s) 1163, 1262
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 531, 650
BpuMI CCSGG 4 cut(s) 169, 225, 239, 294
BsaHI GRCGYC 1 cut(s) 1224
BsaI GGTCTC 2 cut(s) 1164, 1637
BsaJI CCNNGG 3 cut(s) 132, 636, 909
BsaWI WCCGGW 1 cut(s) 1588
Bsc4I CCNNNNNNNGG 8 cut(s) 167, 223, 230, 345, 631, 717, 838, 1595
Bse118I RCCGGY 2 cut(s) 6, 1016
Bse1I ACTGG 3 cut(s) 339, 350, 409
Bse3DI GCAATG 2 cut(s) 1289, 1712
BseAI TCCGGA 1 cut(s) 1588
BseBI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
BseDI CCNNGG 3 cut(s) 132, 636, 909
BseGI GGATG 4 cut(s) 520, 1261, 1327, 1563
BseLI CCNNNNNNNGG 8 cut(s) 167, 223, 230, 345, 631, 717, 838, 1595
BseMI GCAATG 2 cut(s) 1289, 1712
BseMII CTCAG 2 cut(s) 617, 1124
BseNI ACTGG 3 cut(s) 339, 350, 409
BseSI GKGCMC 1 cut(s) 294
BseYI CCCAGC 1 cut(s) 1418
BsgI GTGCAG 1 cut(s) 914
Bsh1236I CGCG 2 cut(s) 638, 1157
Bsh1285I CGRYCG 1 cut(s) 1021
BsiEI CGRYCG 1 cut(s) 1021
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 2 cut(s) 185, 467
BsiSI CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1589
BsiWI CGTACG 1 cut(s) 1022
BslFI GGGAC 3 cut(s) 320, 735, 869
BslI CCNNNNNNNGG 8 cut(s) 167, 223, 230, 345, 631, 717, 838, 1595
BsmAI GTCTC 4 cut(s) 1139, 1164, 1494, 1637
BsmFI GGGAC 3 cut(s) 320, 735, 869
Bso31I GGTCTC 2 cut(s) 1164, 1637
BsoBI CYCGRG 2 cut(s) 185, 467
Bsp1286I GDGCHC 3 cut(s) 294, 468, 626
Bsp13I TCCGGA 1 cut(s) 1588
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
BspCNI CTCAG 2 cut(s) 616, 1125
BspEI TCCGGA 1 cut(s) 1588
BspFNI CGCG 2 cut(s) 638, 1157
BspLI GGNNCC 4 cut(s) 24, 336, 723, 1153
BspMAI CTGCAG 1 cut(s) 1395
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 3 cut(s) 179, 235, 1580
BspQI GCTCTTC 2 cut(s) 467, 837
BspTNI GGTCTC 2 cut(s) 1164, 1637
BsrDI GCAATG 2 cut(s) 1289, 1712
BsrFI RCCGGY 2 cut(s) 6, 1016
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 3 cut(s) 339, 350, 409
BssAI RCCGGY 2 cut(s) 6, 1016
BssECI CCNNGG 3 cut(s) 132, 636, 909
BssMI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
BssNI GRCGYC 1 cut(s) 1224
BssT1I CCWWGG 1 cut(s) 909
Bst2UI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
Bst4CI ACNGT 3 cut(s) 154, 860, 1582
Bst6I CTCTTC 5 cut(s) 319, 467, 837, 1162, 1533
BstACI GRCGYC 1 cut(s) 1224
BstAPI GCANNNNNTGC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 8 cut(s) 626, 682, 691, 748, 876, 1204, 1233, 1317
BstDEI CTNAG 4 cut(s) 603, 764, 888, 1133
BstDSI CCRYGG 1 cut(s) 636
BstF5I GGATG 4 cut(s) 520, 1261, 1327, 1563
BstFNI CGCG 2 cut(s) 638, 1157
BstH2I RGCGCY 1 cut(s) 1238
BstHHI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
BstKTI GATC 9 cut(s) 174, 207, 230, 704, 1161, 1468, 1548, 1588, 1630
BstMAI GTCTC 4 cut(s) 1139, 1164, 1494, 1637
BstMBI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
BstMCI CGRYCG 1 cut(s) 1021
BstNI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
BstNSI RCATGY 2 cut(s) 1319, 1684
BstSCI CCNGG 9 cut(s) 55, 132, 167, 223, 237, 292, 732, 1106, 1364
BstSFI CTRYAG 2 cut(s) 1391, 1578
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 2 cut(s) 638, 1157
BstV2I GAAGAC 1 cut(s) 1626
BstXI CCANNNNNNTGG 1 cut(s) 112
BsuI GTATCC 1 cut(s) 1274
BtgI CCRYGG 1 cut(s) 636
BtrI CACGTC 2 cut(s) 595, 754
BtsCI GGATG 4 cut(s) 520, 1261, 1327, 1563
BtsI GCAGTG 2 cut(s) 843, 1294
BtsIMutI CAGTG 5 cut(s) 346, 402, 693, 843, 1294
Cac8I GCNNGC 8 cut(s) 626, 682, 691, 748, 876, 1204, 1233, 1317
CaiI CAGNNNCTG 2 cut(s) 345, 1486
CfoI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
Cfr10I RCCGGY 2 cut(s) 6, 1016
Cfr13I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
Cfr42I CCGCGG 1 cut(s) 639
CseI GACGC 1 cut(s) 1232
CsiI ACCWGGT 1 cut(s) 55
Csp6I GTAC 4 cut(s) 100, 958, 987, 1023
CspCI CAANNNNNGTGG 2 cut(s) 1163, 1198
CviQI GTAC 4 cut(s) 100, 958, 987, 1023
DdeI CTNAG 4 cut(s) 603, 764, 888, 1133
DpnI GATC 9 cut(s) 173, 206, 229, 703, 1160, 1467, 1547, 1587, 1629
DpnII GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
DraI TTTAAA 1 cut(s) 1210
EaeI YGGCCR 2 cut(s) 748, 1104
Eam1104I CTCTTC 5 cut(s) 319, 467, 837, 1162, 1533
EarI CTCTTC 5 cut(s) 319, 467, 837, 1162, 1533
Eco130I CCWWGG 1 cut(s) 909
Eco147I AGGCCT 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 626
Eco31I GGTCTC 2 cut(s) 1164, 1637
Eco32I GATATC 2 cut(s) 454, 1003
Eco47I GGWCC 3 cut(s) 4, 334, 722
Eco47III AGCGCT 1 cut(s) 1236
Eco57I CTGAAG 3 cut(s) 103, 1380, 1482
Eco88I CYCGRG 2 cut(s) 185, 467
EcoO109I RGGNCCY 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
EcoRV GATATC 2 cut(s) 454, 1003
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 1 cut(s) 626
ErhI CCWWGG 1 cut(s) 909
FaqI GGGAC 3 cut(s) 320, 735, 869
FauI CCCGC 2 cut(s) 633, 933
FbaI TGATCA 2 cut(s) 1545, 1627
FokI GGATG 4 cut(s) 527, 1268, 1334, 1570
FriOI GRGCYC 1 cut(s) 626
FspBI CTAG 3 cut(s) 578, 833, 875
FspI TGCGCA 2 cut(s) 89, 1300
GlaI GCGC 5 cut(s) 89, 848, 1236, 1300, 1389
GsaI CCCAGC 1 cut(s) 1422
GsuI CTGGAG 2 cut(s) 1163, 1262
HaeII RGCGCY 1 cut(s) 1238
HapII CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1589
HgaI GACGC 1 cut(s) 1232
HhaI GCGC 5 cut(s) 90, 849, 1237, 1301, 1390
Hin1I GRCGYC 1 cut(s) 1224
Hin6I GCGC 5 cut(s) 88, 847, 1235, 1299, 1388
HinP1I GCGC 5 cut(s) 88, 847, 1235, 1299, 1388
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HindIII AAGCTT 1 cut(s) 16
HpaII CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1589
HphI GGTGA 8 cut(s) 166, 206, 402, 662, 929, 1132, 1351, 1708
Hpy166II GTNNAC 6 cut(s) 102, 351, 402, 410, 1185, 1222
Hpy188III TCNNGA 6 cut(s) 139, 187, 469, 578, 1589, 1631
Hpy8I GTNNAC 6 cut(s) 102, 351, 402, 410, 1185, 1222
Hpy99I CGWCG 3 cut(s) 758, 1652, 1666
HpyAV CCTTC 8 cut(s) 127, 227, 277, 492, 846, 1085, 1240, 1684
HpyCH4III ACNGT 3 cut(s) 154, 860, 1582
HpyCH4IV ACGT 4 cut(s) 93, 594, 753, 1593
HpyF3I CTNAG 4 cut(s) 603, 764, 888, 1133
HpySE526I ACGT 4 cut(s) 93, 594, 753, 1593
Hsp92I GRCGYC 1 cut(s) 1224
HspAI GCGC 5 cut(s) 88, 847, 1235, 1299, 1388
Kpn2I TCCGGA 1 cut(s) 1588
Ksp22I TGATCA 2 cut(s) 1545, 1627
KspI CCGCGG 1 cut(s) 639
Kzo9I GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
LguI GCTCTTC 2 cut(s) 467, 837
LmnI GCTCC 4 cut(s) 22, 296, 743, 1349
LweI GCATC 4 cut(s) 319, 787, 1345, 1608
MabI ACCWGGT 1 cut(s) 55
MaeI CTAG 3 cut(s) 578, 833, 875
MaeII ACGT 4 cut(s) 93, 594, 753, 1593
MaeIII GTNAC 5 cut(s) 68, 341, 917, 1052, 1381
MalI GATC 9 cut(s) 173, 206, 229, 703, 1160, 1467, 1547, 1587, 1629
MboI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
MhlI GDGCHC 3 cut(s) 294, 468, 626
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 4 cut(s) 31, 581, 777, 1128
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 6 cut(s) 236, 257, 494, 716, 1064, 1412
Mox20I TGGCCA 1 cut(s) 750
MroI TCCGGA 1 cut(s) 1588
MscI TGGCCA 1 cut(s) 750
MseI TTAA 2 cut(s) 780, 1209
MslI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 5 cut(s) 384, 571, 638, 928, 1304
MspI CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1589
MspR9I CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
MvaI CCWGG 5 cut(s) 57, 134, 734, 1108, 1366
MvnI CGCG 2 cut(s) 638, 1157
NciI CCSGG 4 cut(s) 169, 225, 239, 294
NdeII GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
NheI GCTAGC 1 cut(s) 874
NlaIV GGNNCC 4 cut(s) 24, 336, 723, 1153
NmeAIII GCCGAG 1 cut(s) 1426
NmuCI GTSAC 4 cut(s) 341, 917, 1052, 1381
NsbI TGCGCA 2 cut(s) 89, 1300
NspI RCATGY 2 cut(s) 1319, 1684
PaeI GCATGC 1 cut(s) 1319
PaeR7I CTCGAG 2 cut(s) 185, 467
PceI AGGCCT 1 cut(s) 160
PciI ACATGT 1 cut(s) 1680
PciSI GCTCTTC 2 cut(s) 467, 837
PcsI WCGNNNNNNNCGW 3 cut(s) 474, 591, 1647
PfeI GAWTC 5 cut(s) 142, 509, 1307, 1432, 1483
Pfl23II CGTACG 1 cut(s) 1022
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 2 cut(s) 167, 237
PleI GAGTC 6 cut(s) 236, 257, 494, 715, 1064, 1411
PpsI GAGTC 6 cut(s) 236, 257, 494, 715, 1064, 1411
PpuMI RGGWCCY 1 cut(s) 722
PscI ACATGT 1 cut(s) 1680
PsiI TTATAA 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
PspFI CCCAGC 1 cut(s) 1418
PspGI CCWGG 5 cut(s) 55, 132, 732, 1106, 1364
PspLI CGTACG 1 cut(s) 1022
PspN4I GGNNCC 4 cut(s) 24, 336, 723, 1153
PspPI GGNCC 5 cut(s) 4, 232, 334, 722, 1152
PspPPI RGGWCCY 1 cut(s) 722
PstI CTGCAG 1 cut(s) 1395
PstNI CAGNNNCTG 2 cut(s) 345, 1486
PsyI GACNNNGTC 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 4 cut(s) 101, 959, 988, 1024
RsaNI GTAC 4 cut(s) 100, 958, 987, 1023
RseI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SacII CCGCGG 1 cut(s) 639
SapI GCTCTTC 2 cut(s) 467, 837
SaqAI TTAA 2 cut(s) 780, 1209
Sau3AI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1465, 1545, 1585, 1627
Sau96I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
SchI GAGTC 6 cut(s) 236, 257, 494, 716, 1064, 1412
ScrFI CCNGG 9 cut(s) 57, 134, 169, 225, 239, 294, 734, 1108, 1366
SduI GDGCHC 3 cut(s) 294, 468, 626
SexAI ACCWGGT 1 cut(s) 55
SfaNI GCATC 4 cut(s) 319, 787, 1345, 1608
SfcI CTRYAG 2 cut(s) 1391, 1578
Sfr274I CTCGAG 2 cut(s) 185, 467
Sfr303I CCGCGG 1 cut(s) 639
SgrBI CCGCGG 1 cut(s) 639
SinI GGWCC 3 cut(s) 4, 334, 722
SlaI CTCGAG 2 cut(s) 185, 467
SmiMI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SmlI CTYRAG 4 cut(s) 185, 467, 546, 629
SmoI CTYRAG 4 cut(s) 185, 467, 546, 629
SphI GCATGC 1 cut(s) 1319
Sse9I AATT 4 cut(s) 31, 581, 777, 1128
SseBI AGGCCT 1 cut(s) 160
SspI AATATT 1 cut(s) 1672
SspMI CTAG 3 cut(s) 578, 833, 875
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 9 cut(s) 55, 132, 167, 223, 237, 292, 732, 1106, 1364
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 3 cut(s) 154, 860, 1582
TaiI ACGT 4 cut(s) 96, 597, 756, 1596
TaqI TCGA 9 cut(s) 186, 320, 327, 468, 585, 620, 666, 756, 1688
TaqII GACCGA 1 cut(s) 1661
TasI AATT 4 cut(s) 31, 581, 777, 1128
TatI WGTACW 2 cut(s) 99, 957
TauI GCSGC 6 cut(s) 638, 641, 941, 1043, 1106, 1157
TfiI GAWTC 5 cut(s) 142, 509, 1307, 1432, 1483
Tru1I TTAA 2 cut(s) 780, 1209
Tru9I TTAA 2 cut(s) 780, 1209
TscAI CASTG 5 cut(s) 346, 409, 700, 843, 1301
TseFI GTSAC 4 cut(s) 341, 917, 1052, 1381
Tsp45I GTSAC 4 cut(s) 341, 917, 1052, 1381
TspDTI ATGAA 4 cut(s) 306, 364, 474, 1493
TspGWI ACGGA 5 cut(s) 61, 828, 1179, 1293, 1679
TspRI CASTG 5 cut(s) 346, 409, 700, 843, 1301
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 4, 334, 722
XapI RAATTY 2 cut(s) 31, 581
XbaI TCTAGA 1 cut(s) 577
XceI RCATGY 2 cut(s) 1319, 1684
XhoI CTCGAG 2 cut(s) 185, 467
XspI CTAG 3 cut(s) 578, 833, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.