Rmu_co8439577.1_g000001

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8439577.1
OG0013599
Physical Location & Seq
Forward (+)
632 .. 1575
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8439577.1_g000001.1.cds

Sequence Viewer

Length: 944 bp
atggaccggctcataagcttggagccatcaaatttcgttgcaatacggattgaaccgggtcagaaatgttacggcctactcactttgcgcaatgtcatgtacaccatgcctgtggccttcaggcttcaagccctggtcaagaatcgctacacggtgagacctcaatccgggatcatatctccgctcgagaagctaaccattgagatcgtctatcaccttccaccgggatcgggcctcccggattcgttcccttattgccatgactccttccttttgcatagcgtggtcgtgcccggagcagccatcaaaaactcttcatcgactttcgatgcggtccccagtgactggttcacaacaaagaagaagcaagtgttcatagacagcggtgtgaagattatgttcattggttcacctgttttggcacaacttgtgtccgatggtttgatggacgatatcagagaagtgctcgagaagagcgacccttcatggaaagcagccgactctgtggattcggatggtcaatctttacttcacttggctattgctcaaggccgacccgacctcgttcagctgcttctcgaattcaagccagacgtggaggctcagagccgttcggggtcgagcccgcttgaggccgcggctgcttcgggtgaggctttgatagtcgagcttctgttggctcgtcgtgcaagcactgaaagatcagagtcctccacttggggacctatccacctggcagccggagctggccacgtcgaagttctcaggcttcttataattaagggggctaatgttgatgctcaaacaaaagacggaaacaccgccttacacctagcagtggaagagcgtagaagggactgtgcgaggcttttgctagcaagtggtgctaaggccgaagttcgtaatagcaaggagggtgacaccccgctgcacatagccgccgg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

315

Amino Acids

34.07

Weight (kDa)

6.08

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
Acc16I TGCGCA 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 1 cut(s) 184
AccII CGCG 1 cut(s) 638
AciI CCGC 9 cut(s) 182, 332, 384, 626, 636, 638, 822, 926, 939
AclWI GGATC 2 cut(s) 179, 235
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 2 cut(s) 31, 581
AcuI CTGAAG 1 cut(s) 103
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
AgsI TTSAA 3 cut(s) 53, 128, 586
AjiI CACGTC 2 cut(s) 595, 754
AjnI CCWGG 2 cut(s) 132, 732
AluBI AGCT 5 cut(s) 18, 193, 571, 670, 746
AluI AGCT 5 cut(s) 18, 193, 571, 670, 746
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 2 cut(s) 179, 235
AlwNI CAGNNNCTG 1 cut(s) 345
Ama87I CYCGRG 2 cut(s) 185, 467
AoxI GGCC 7 cut(s) 73, 114, 232, 550, 633, 748, 891
ApeKI GCWGC 6 cut(s) 299, 494, 571, 641, 737, 928
ApoI RAATTY 2 cut(s) 31, 581
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 4 cut(s) 4, 232, 334, 722
AsuC2I CCSGG 5 cut(s) 57, 169, 225, 239, 294
AsuHPI GGTGA 5 cut(s) 166, 206, 402, 662, 929
AsuNHI GCTAGC 1 cut(s) 874
AvaI CYCGRG 2 cut(s) 185, 467
AvaII GGWCC 3 cut(s) 4, 334, 722
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 750
BanII GRGCYC 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 6 cut(s) 311, 506, 558, 628, 749, 915
BccI CCATC 5 cut(s) 34, 311, 431, 439, 509
BceAI ACGGC 2 cut(s) 88, 594
BcgI CGANNNNNNTGC 2 cut(s) 853, 887
BciT130I CCWGG 2 cut(s) 134, 734
BcnI CCSGG 5 cut(s) 57, 169, 225, 239, 294
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 2 cut(s) 833, 875
BisI GCNGC 9 cut(s) 300, 495, 572, 636, 639, 642, 738, 929, 939
BlsI GCNGC 9 cut(s) 301, 496, 573, 637, 640, 643, 739, 930, 940
Bme1390I CCNGG 7 cut(s) 57, 134, 169, 225, 239, 294, 734
Bme18I GGWCC 3 cut(s) 4, 334, 722
BmeT110I CYCGRG 2 cut(s) 185, 467
BmgBI CACGTC 2 cut(s) 595, 754
BmgT120I GGNCC 4 cut(s) 4, 232, 334, 722
BmiI GGNNCC 3 cut(s) 24, 336, 723
BmrFI CCNGG 7 cut(s) 57, 134, 169, 225, 239, 294, 734
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 2 cut(s) 319, 787
BmtI GCTAGC 1 cut(s) 878
BmuI ACTGGG 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 450, 482
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 531, 650
BpuMI CCSGG 5 cut(s) 57, 169, 225, 239, 294
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 2 cut(s) 132, 636
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
Bse118I RCCGGY 1 cut(s) 6
Bse1I ACTGG 2 cut(s) 339, 350
Bse3DI GCAATG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 134, 734
BseDI CCNNGG 2 cut(s) 132, 636
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 2 cut(s) 617, 778
BseNI ACTGG 2 cut(s) 339, 350
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 6 cut(s) 311, 506, 558, 628, 749, 915
BsgI GTGCAG 1 cut(s) 914
Bsh1236I CGCG 1 cut(s) 638
BshFI GGCC 7 cut(s) 75, 116, 234, 552, 635, 750, 893
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 2 cut(s) 185, 467
BsiSI CCGG 8 cut(s) 7, 56, 168, 224, 239, 294, 741, 942
BslFI GGGAC 3 cut(s) 320, 735, 869
BslI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 3 cut(s) 320, 735, 869
BsnI GGCC 7 cut(s) 75, 116, 234, 552, 635, 750, 893
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 2 cut(s) 185, 467
Bsp1286I GDGCHC 3 cut(s) 294, 468, 626
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 4 cut(s) 171, 204, 227, 701
BspACI CCGC 9 cut(s) 182, 332, 384, 626, 636, 638, 822, 926, 939
BspANI GGCC 7 cut(s) 75, 116, 234, 552, 635, 750, 893
BspCNI CTCAG 2 cut(s) 616, 777
BspFNI CGCG 1 cut(s) 638
BspLI GGNNCC 3 cut(s) 24, 336, 723
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 2 cut(s) 179, 235
BspQI GCTCTTC 2 cut(s) 467, 837
BspTNI GGTCTC 1 cut(s) 151
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 2 cut(s) 339, 350
BssAI RCCGGY 1 cut(s) 6
BssECI CCNNGG 2 cut(s) 132, 636
BssMI GATC 4 cut(s) 171, 204, 227, 701
Bst2UI CCWGG 2 cut(s) 134, 734
Bst4CI ACNGT 2 cut(s) 154, 860
Bst6I CTCTTC 3 cut(s) 319, 467, 837
BstAPI GCANNNNNTGC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 4 cut(s) 626, 691, 748, 876
BstDEI CTNAG 3 cut(s) 603, 764, 888
BstDSI CCRYGG 1 cut(s) 636
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 1 cut(s) 638
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 4 cut(s) 174, 207, 230, 704
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 4 cut(s) 171, 204, 227, 701
BstMWI GCNNNNNNNGC 5 cut(s) 190, 641, 686, 743, 884
BstNI CCWGG 2 cut(s) 134, 734
BstSCI CCNGG 7 cut(s) 55, 132, 167, 223, 237, 292, 732
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 1 cut(s) 638
BstV1I GCAGC 6 cut(s) 311, 506, 558, 628, 749, 915
BstXI CCANNNNNNTGG 1 cut(s) 112
BsuRI GGCC 7 cut(s) 75, 116, 234, 552, 635, 750, 893
BtgI CCRYGG 1 cut(s) 636
BtrI CACGTC 2 cut(s) 595, 754
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 3 cut(s) 346, 693, 843
Cac8I GCNNGC 4 cut(s) 626, 691, 748, 876
CaiI CAGNNNCTG 1 cut(s) 345
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 1 cut(s) 6
Cfr13I GGNCC 4 cut(s) 4, 232, 334, 722
Cfr42I CCGCGG 1 cut(s) 639
Csp6I GTAC 1 cut(s) 100
CviAII CATG 4 cut(s) 97, 106, 260, 486
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 3 cut(s) 603, 764, 888
DpnI GATC 4 cut(s) 173, 206, 229, 703
DpnII GATC 4 cut(s) 171, 204, 227, 701
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 3 cut(s) 319, 467, 837
EarI CTCTTC 3 cut(s) 319, 467, 837
Eco24I GRGCYC 1 cut(s) 626
Eco31I GGTCTC 1 cut(s) 151
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 3 cut(s) 4, 334, 722
Eco57I CTGAAG 1 cut(s) 103
Eco88I CYCGRG 2 cut(s) 185, 467
EcoO109I RGGNCCY 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 132, 732
EcoRV GATATC 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 626
FaeI CATG 4 cut(s) 100, 109, 263, 489
FaqI GGGAC 3 cut(s) 320, 735, 869
FatI CATG 4 cut(s) 96, 105, 259, 485
FauI CCCGC 2 cut(s) 633, 933
Fnu4HI GCNGC 9 cut(s) 300, 495, 572, 636, 639, 642, 738, 929, 939
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 626
Fsp4HI GCNGC 9 cut(s) 300, 495, 572, 636, 639, 642, 738, 929, 939
FspBI CTAG 2 cut(s) 833, 875
FspI TGCGCA 1 cut(s) 89
GlaI GCGC 1 cut(s) 89
GluI GCNGC 9 cut(s) 300, 495, 572, 636, 639, 642, 738, 929, 939
HaeIII GGCC 7 cut(s) 75, 116, 234, 552, 635, 750, 893
HapII CCGG 8 cut(s) 7, 56, 168, 224, 239, 294, 741, 942
HhaI GCGC 1 cut(s) 90
Hin1II CATG 4 cut(s) 100, 109, 263, 489
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 16
HinfI GANTC 6 cut(s) 142, 242, 263, 500, 509, 707
HpaII CCGG 8 cut(s) 7, 56, 168, 224, 239, 294, 741, 942
HphI GGTGA 5 cut(s) 166, 206, 402, 662, 929
Hpy166II GTNNAC 3 cut(s) 102, 351, 410
Hpy188I TCNGA 6 cut(s) 63, 436, 458, 514, 606, 706
Hpy188III TCNNGA 4 cut(s) 139, 187, 469, 578
Hpy8I GTNNAC 3 cut(s) 102, 351, 410
Hpy99I CGWCG 2 cut(s) 687, 758
HpyAV CCTTC 5 cut(s) 127, 227, 277, 492, 846
HpyCH4III ACNGT 2 cut(s) 154, 860
HpyCH4IV ACGT 2 cut(s) 594, 753
HpyCH4V TGCA 4 cut(s) 41, 277, 689, 931
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 641, 686, 743, 884
HpyF3I CTNAG 3 cut(s) 603, 764, 888
HpySE526I ACGT 2 cut(s) 594, 753
Hsp92II CATG 4 cut(s) 100, 109, 263, 489
HspAI GCGC 1 cut(s) 88
KspI CCGCGG 1 cut(s) 639
Kzo9I GATC 4 cut(s) 171, 204, 227, 701
LguI GCTCTTC 2 cut(s) 467, 837
LmnI GCTCC 3 cut(s) 22, 296, 743
Lsp1109I GCAGC 6 cut(s) 311, 506, 558, 628, 749, 915
LweI GCATC 2 cut(s) 319, 787
MaeI CTAG 2 cut(s) 833, 875
MaeII ACGT 2 cut(s) 594, 753
MaeIII GTNAC 3 cut(s) 68, 341, 917
MalI GATC 4 cut(s) 173, 206, 229, 703
MbiI CCGCTC 1 cut(s) 184
MboI GATC 4 cut(s) 171, 204, 227, 701
MboII GAAGA 5 cut(s) 306, 373, 403, 484, 854
MhlI GDGCHC 3 cut(s) 294, 468, 626
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 3 cut(s) 31, 581, 777
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 3 cut(s) 257, 494, 716
MnlI CCTC 9 cut(s) 171, 245, 572, 592, 625, 646, 721, 858, 907
Mox20I TGGCCA 1 cut(s) 750
MscI TGGCCA 1 cut(s) 750
MseI TTAA 1 cut(s) 780
MslI CAYNNNNRTG 1 cut(s) 110
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 4 cut(s) 384, 571, 638, 928
MspI CCGG 8 cut(s) 7, 56, 168, 224, 239, 294, 741, 942
MspR9I CCNGG 7 cut(s) 57, 134, 169, 225, 239, 294, 734
MvaI CCWGG 2 cut(s) 134, 734
MvnI CGCG 1 cut(s) 638
MwoI GCNNNNNNNGC 5 cut(s) 190, 641, 686, 743, 884
NciI CCSGG 5 cut(s) 57, 169, 225, 239, 294
NdeII GATC 4 cut(s) 171, 204, 227, 701
NheI GCTAGC 1 cut(s) 874
NlaIII CATG 4 cut(s) 100, 109, 263, 489
NlaIV GGNNCC 3 cut(s) 24, 336, 723
NmuCI GTSAC 2 cut(s) 341, 917
NsbI TGCGCA 1 cut(s) 89
PaeR7I CTCGAG 2 cut(s) 185, 467
PciSI GCTCTTC 2 cut(s) 467, 837
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PfeI GAWTC 3 cut(s) 142, 242, 509
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 2 cut(s) 167, 237
PkrI GCNGC 9 cut(s) 301, 496, 573, 637, 640, 643, 739, 930, 940
PleI GAGTC 3 cut(s) 257, 494, 715
PpsI GAGTC 3 cut(s) 257, 494, 715
PpuMI RGGWCCY 1 cut(s) 722
PsiI TTATAA 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 132, 732
PspGI CCWGG 2 cut(s) 132, 732
PspN4I GGNNCC 3 cut(s) 24, 336, 723
PspPI GGNCC 4 cut(s) 4, 232, 334, 722
PspPPI RGGWCCY 1 cut(s) 722
PstNI CAGNNNCTG 1 cut(s) 345
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 110
SacII CCGCGG 1 cut(s) 639
SapI GCTCTTC 2 cut(s) 467, 837
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 9 cut(s) 300, 495, 572, 636, 639, 642, 738, 929, 939
Sau3AI GATC 4 cut(s) 171, 204, 227, 701
Sau96I GGNCC 4 cut(s) 4, 232, 334, 722
SchI GAGTC 3 cut(s) 257, 494, 716
ScrFI CCNGG 7 cut(s) 57, 134, 169, 225, 239, 294, 734
SduI GDGCHC 3 cut(s) 294, 468, 626
SfaNI GCATC 2 cut(s) 319, 787
Sfr274I CTCGAG 2 cut(s) 185, 467
Sfr303I CCGCGG 1 cut(s) 639
SgrBI CCGCGG 1 cut(s) 639
SinI GGWCC 3 cut(s) 4, 334, 722
SlaI CTCGAG 2 cut(s) 185, 467
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 4 cut(s) 185, 467, 546, 629
SmoI CTYRAG 4 cut(s) 185, 467, 546, 629
Sse9I AATT 3 cut(s) 31, 581, 777
SsiI CCGC 9 cut(s) 182, 332, 384, 626, 636, 638, 822, 926, 939
SspMI CTAG 2 cut(s) 833, 875
StyD4I CCNGG 7 cut(s) 55, 132, 167, 223, 237, 292, 732
TaaI ACNGT 2 cut(s) 154, 860
TaiI ACGT 2 cut(s) 597, 756
TaqI TCGA 8 cut(s) 186, 320, 327, 468, 579, 620, 666, 756
TasI AATT 3 cut(s) 31, 581, 777
TatI WGTACW 1 cut(s) 99
TauI GCSGC 3 cut(s) 638, 641, 941
TfiI GAWTC 3 cut(s) 142, 242, 509
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 3 cut(s) 346, 700, 843
TseFI GTSAC 2 cut(s) 341, 917
TseI GCWGC 6 cut(s) 299, 494, 571, 641, 737, 928
Tsp45I GTSAC 2 cut(s) 341, 917
TspDTI ATGAA 4 cut(s) 306, 364, 391, 474
TspGWI ACGGA 2 cut(s) 61, 828
TspRI CASTG 3 cut(s) 346, 700, 843
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 4, 334, 722
XapI RAATTY 2 cut(s) 31, 581
XhoI CTCGAG 2 cut(s) 185, 467
XspI CTAG 2 cut(s) 833, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.