Rmu_sc0001127.1_g000020

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001127.1
OG0013599
Physical Location & Seq
Forward (+)
84855 .. 86492
1638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001127.1_g000020.1.cds

Sequence Viewer

Length: 1638 bp
atggaccggctcataagcttggagccatcaaatttcgttgcaatacggattgaaccgggtcagaaatgttacggcctactcactttgcgcaatgtcatgtacaccatgcctgtggccttcaggcttcaagccctggtcaagaatcgctacacggtgagacctcaatccgggatcatatctccgctcgagaagctaaccattgagatcgtctatcaccttccaccgggatcgggcctcccggattcgttcccttattgccatgactccttccttttgcatagcgtggtcgtgcccggagcagccatcaaaaactcttcatcgactttcgatgcggtccccagtgactggttcacaacaaagaagaagcaagtgttcatagacagcggtgtgaagattatgttcattggttcacctgttttggcacaacttgtgtccgatggtttgatggacgatatcagagaagtgctcgagaagagcgacccttcatggaaagcagccgactctgtggattcggatggtcaatctttacttcacttggctattgctcaaggccgacccgacctcgttcagctgcttctcgaattcaagccagacgtggaggctcagagccgttcggggtcgagcccgcttgaggccgcggctgcttcgggtgaggctttgatagtcgagcttctgttggctcgtcgtgcaagcactgaaagatcagagtcctccacttggggacctatccacctggcagccggagctggccacgtcgaagttctcaggcttcttataattaagggggctaatgttgatgctcaaacaaaagacggaaacaccgccttacacctagcagtggaagagcgtagaagggactgtgcgaggcttttgctagcaagtggtgctaaggccgaagttcgtaatagcaaggagggtgacaccccgctgcacatagccgccggcttgggagatgagtacatggtcaagctgttgctgcaaaagggtactgccaataaagatatcagaaactacgccggtaaaacggcttatgatgttgcggcagagaatggtcacaccaagctctttgactcccttcggcttggagatagcttgtgtatagcggccaggaaaggagaggtgagaacaattctgaggctgctggagaccggggccgcgatcaacggaagagaccaacatgggtggactgctctgcatagggcgtgctttaaaggcaaagttgacgccgtgcgatcgctgctggagaagggtgtggatgtggatgctaaagatgaggatggatacacggcattgcactgcgcagcggaatcggggcatgcggatgtgatagatatgttggtgaagaaaggagctgatgctgaagccaggactaataagggtgtgactgcgctgcagattgccgagtctttgcactatgctgggattacaaggattcttatgacacatggaggtggtgcaataaaagatcagaacatggcacagattctgtctcacgcttcagttcattcgtttgggaagaaatccatgggcgttgaggaagagatgatcaagggagggatgatggtgaggaaaaaatcgactcgagctagagctcttcgtggtagctttgatcgttctgtgcccttggctgtgctttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

545

Amino Acids

58.86

Weight (kDa)

8.1

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
Acc16I TGCGCA 2 cut(s) 89, 1300
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 1 cut(s) 184
AccII CGCG 2 cut(s) 638, 1157
AclWI GGATC 2 cut(s) 179, 235
AcoI YGGCCR 2 cut(s) 748, 1104
AcsI RAATTY 2 cut(s) 31, 581
AcuI CTGAAG 3 cut(s) 103, 1380, 1482
AcyI GRCGYC 1 cut(s) 1224
AfaI GTAC 3 cut(s) 101, 959, 988
AfiI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
AgsI TTSAA 3 cut(s) 53, 128, 586
AjiI CACGTC 2 cut(s) 595, 754
AjnI CCWGG 4 cut(s) 132, 732, 1106, 1364
Alw21I GWGCWC 2 cut(s) 468, 1594
Alw26I GTCTC 4 cut(s) 151, 1139, 1164, 1494
AlwI GGATC 2 cut(s) 179, 235
AlwNI CAGNNNCTG 2 cut(s) 345, 1486
Ama87I CYCGRG 3 cut(s) 185, 467, 1581
AoxI GGCC 9 cut(s) 73, 114, 232, 550, 633, 748, 891, 1104, 1152
ApoI RAATTY 2 cut(s) 31, 581
AsiSI GCGATCGC 1 cut(s) 1235
AspLEI GCGC 3 cut(s) 90, 1301, 1390
AspS9I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
AsuC2I CCSGG 6 cut(s) 57, 169, 225, 239, 294, 1150
AsuHPI GGTGA 8 cut(s) 166, 206, 402, 662, 929, 1132, 1351, 1576
AsuNHI GCTAGC 1 cut(s) 874
AvaI CYCGRG 3 cut(s) 185, 467, 1581
AvaII GGWCC 3 cut(s) 4, 334, 722
BaeGI GKGCMC 2 cut(s) 294, 1623
BalI TGGCCA 1 cut(s) 750
BanII GRGCYC 2 cut(s) 626, 1594
Bbv12I GWGCWC 2 cut(s) 468, 1594
BccI CCATC 7 cut(s) 34, 311, 431, 439, 509, 1271, 1555
BceAI ACGGC 5 cut(s) 88, 594, 1041, 1211, 1302
BcgI CGANNNNNNTGC 4 cut(s) 853, 887, 1290, 1324
BciT130I CCWGG 4 cut(s) 134, 734, 1108, 1366
BciVI GTATCC 1 cut(s) 1274
BclI TGATCA 1 cut(s) 1545
BcnI CCSGG 6 cut(s) 57, 169, 225, 239, 294, 1150
BcoDI GTCTC 4 cut(s) 151, 1139, 1164, 1494
BfaI CTAG 3 cut(s) 833, 875, 1587
BfmI CTRYAG 1 cut(s) 1391
BfuI GTATCC 1 cut(s) 1274
Bme18I GGWCC 3 cut(s) 4, 334, 722
BmeT110I CYCGRG 3 cut(s) 185, 467, 1581
BmgBI CACGTC 2 cut(s) 595, 754
BmgT120I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
BmiI GGNNCC 4 cut(s) 24, 336, 723, 1153
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 4 cut(s) 319, 787, 1252, 1345
BmtI GCTAGC 1 cut(s) 878
BmuI ACTGGG 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpmI CTGGAG 2 cut(s) 1163, 1262
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 531, 650
BpuMI CCSGG 6 cut(s) 57, 169, 225, 239, 294, 1150
BsaHI GRCGYC 1 cut(s) 1224
BsaI GGTCTC 3 cut(s) 151, 1139, 1164
BsaJI CCNNGG 5 cut(s) 132, 636, 1149, 1524, 1623
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
Bse118I RCCGGY 3 cut(s) 6, 941, 1016
Bse1I ACTGG 2 cut(s) 339, 350
Bse3DI GCAATG 2 cut(s) 97, 1289
BseBI CCWGG 4 cut(s) 134, 734, 1108, 1366
BseDI CCNNGG 5 cut(s) 132, 636, 1149, 1524, 1623
BseGI GGATG 6 cut(s) 520, 1261, 1267, 1282, 1327, 1563
BseLI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
BseMI GCAATG 2 cut(s) 97, 1289
BseMII CTCAG 3 cut(s) 617, 778, 1124
BseNI ACTGG 2 cut(s) 339, 350
BseSI GKGCMC 2 cut(s) 294, 1623
BseYI CCCAGC 1 cut(s) 1418
BsgI GTGCAG 1 cut(s) 914
Bsh1236I CGCG 2 cut(s) 638, 1157
Bsh1285I CGRYCG 1 cut(s) 1235
BshFI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
BsiEI CGRYCG 1 cut(s) 1235
BsiHKAI GWGCWC 2 cut(s) 468, 1594
BsiHKCI CYCGRG 3 cut(s) 185, 467, 1581
BslFI GGGAC 3 cut(s) 320, 735, 869
BslI CCNNNNNNNGG 7 cut(s) 167, 223, 230, 345, 631, 717, 838
BsmAI GTCTC 4 cut(s) 151, 1139, 1164, 1494
BsmFI GGGAC 3 cut(s) 320, 735, 869
BsnI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
Bso31I GGTCTC 3 cut(s) 151, 1139, 1164
BsoBI CYCGRG 3 cut(s) 185, 467, 1581
Bsp1286I GDGCHC 5 cut(s) 294, 468, 626, 1594, 1623
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
Bsp19I CCATGG 1 cut(s) 1524
BspANI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
BspCNI CTCAG 3 cut(s) 616, 777, 1125
BspFNI CGCG 2 cut(s) 638, 1157
BspLI GGNNCC 4 cut(s) 24, 336, 723, 1153
BspMAI CTGCAG 1 cut(s) 1395
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 2 cut(s) 179, 235
BspQI GCTCTTC 3 cut(s) 467, 837, 1599
BspTNI GGTCTC 3 cut(s) 151, 1139, 1164
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 97, 1289
BsrFI RCCGGY 3 cut(s) 6, 941, 1016
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 2 cut(s) 339, 350
BssAI RCCGGY 3 cut(s) 6, 941, 1016
BssECI CCNNGG 5 cut(s) 132, 636, 1149, 1524, 1623
BssMI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
BssNI GRCGYC 1 cut(s) 1224
BssT1I CCWWGG 2 cut(s) 1524, 1623
Bst2UI CCWGG 4 cut(s) 134, 734, 1108, 1366
Bst4CI ACNGT 2 cut(s) 154, 860
Bst6I CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1599
BstACI GRCGYC 1 cut(s) 1224
BstAPI GCANNNNNTGC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 7 cut(s) 626, 691, 748, 876, 943, 1204, 1317
BstDEI CTNAG 4 cut(s) 603, 764, 888, 1133
BstDSI CCRYGG 2 cut(s) 636, 1524
BstF5I GGATG 6 cut(s) 520, 1261, 1267, 1282, 1327, 1563
BstFNI CGCG 2 cut(s) 638, 1157
BstHHI GCGC 3 cut(s) 90, 1301, 1390
BstKTI GATC 9 cut(s) 174, 207, 230, 704, 1161, 1235, 1468, 1548, 1612
BstMAI GTCTC 4 cut(s) 151, 1139, 1164, 1494
BstMBI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
BstMCI CGRYCG 1 cut(s) 1235
BstMWI GCNNNNNNNGC 8 cut(s) 190, 641, 686, 743, 884, 976, 1212, 1237
BstNI CCWGG 4 cut(s) 134, 734, 1108, 1366
BstNSI RCATGY 1 cut(s) 1319
BstSFI CTRYAG 1 cut(s) 1391
BstSLI GKGCMC 2 cut(s) 294, 1623
BstUI CGCG 2 cut(s) 638, 1157
BstXI CCANNNNNNTGG 1 cut(s) 112
BsuI GTATCC 1 cut(s) 1274
BsuRI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
BtgI CCRYGG 2 cut(s) 636, 1524
BtrI CACGTC 2 cut(s) 595, 754
BtsCI GGATG 6 cut(s) 520, 1261, 1267, 1282, 1327, 1563
BtsI GCAGTG 2 cut(s) 843, 1294
BtsIMutI CAGTG 4 cut(s) 346, 693, 843, 1294
Cac8I GCNNGC 7 cut(s) 626, 691, 748, 876, 943, 1204, 1317
CaiI CAGNNNCTG 2 cut(s) 345, 1486
CfoI GCGC 3 cut(s) 90, 1301, 1390
Cfr10I RCCGGY 3 cut(s) 6, 941, 1016
Cfr13I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
Cfr42I CCGCGG 1 cut(s) 639
CseI GACGC 1 cut(s) 1232
Csp6I GTAC 3 cut(s) 100, 958, 987
CspCI CAANNNNNGTGG 2 cut(s) 1163, 1198
CviQI GTAC 3 cut(s) 100, 958, 987
DdeI CTNAG 4 cut(s) 603, 764, 888, 1133
DpnI GATC 9 cut(s) 173, 206, 229, 703, 1160, 1234, 1467, 1547, 1611
DpnII GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
DraI TTTAAA 1 cut(s) 1210
EaeI YGGCCR 2 cut(s) 748, 1104
Eam1104I CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1599
EarI CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1599
Ecl136II GAGCTC 1 cut(s) 1592
Eco130I CCWWGG 2 cut(s) 1524, 1623
Eco24I GRGCYC 2 cut(s) 626, 1594
Eco31I GGTCTC 3 cut(s) 151, 1139, 1164
Eco32I GATATC 2 cut(s) 454, 1003
Eco47I GGWCC 3 cut(s) 4, 334, 722
Eco53kI GAGCTC 1 cut(s) 1592
Eco57I CTGAAG 3 cut(s) 103, 1380, 1482
Eco88I CYCGRG 3 cut(s) 185, 467, 1581
EcoICRI GAGCTC 1 cut(s) 1592
EcoO109I RGGNCCY 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 4 cut(s) 132, 732, 1106, 1364
EcoRV GATATC 2 cut(s) 454, 1003
EcoT14I CCWWGG 2 cut(s) 1524, 1623
EcoT38I GRGCYC 2 cut(s) 626, 1594
ErhI CCWWGG 2 cut(s) 1524, 1623
FaqI GGGAC 3 cut(s) 320, 735, 869
FauI CCCGC 2 cut(s) 633, 933
FbaI TGATCA 1 cut(s) 1545
FokI GGATG 6 cut(s) 527, 1268, 1274, 1289, 1334, 1570
FriOI GRGCYC 2 cut(s) 626, 1594
FspBI CTAG 3 cut(s) 833, 875, 1587
FspI TGCGCA 2 cut(s) 89, 1300
GlaI GCGC 3 cut(s) 89, 1300, 1389
GsaI CCCAGC 1 cut(s) 1422
GsuI CTGGAG 2 cut(s) 1163, 1262
HaeIII GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
HgaI GACGC 1 cut(s) 1232
HhaI GCGC 3 cut(s) 90, 1301, 1390
Hin1I GRCGYC 1 cut(s) 1224
Hin6I GCGC 3 cut(s) 88, 1299, 1388
HinP1I GCGC 3 cut(s) 88, 1299, 1388
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HindIII AAGCTT 1 cut(s) 16
HphI GGTGA 8 cut(s) 166, 206, 402, 662, 929, 1132, 1351, 1576
Hpy166II GTNNAC 5 cut(s) 102, 351, 410, 1185, 1222
Hpy188I TCNGA 9 cut(s) 63, 436, 458, 514, 606, 706, 1007, 1134, 1470
Hpy188III TCNNGA 4 cut(s) 139, 187, 469, 578
Hpy8I GTNNAC 5 cut(s) 102, 351, 410, 1185, 1222
Hpy99I CGWCG 2 cut(s) 687, 758
HpyAV CCTTC 7 cut(s) 127, 227, 277, 492, 846, 1085, 1240
HpyCH4III ACNGT 2 cut(s) 154, 860
HpyCH4IV ACGT 2 cut(s) 594, 753
HpyF10VI GCNNNNNNNGC 8 cut(s) 190, 641, 686, 743, 884, 976, 1212, 1237
HpyF3I CTNAG 4 cut(s) 603, 764, 888, 1133
HpySE526I ACGT 2 cut(s) 594, 753
Hsp92I GRCGYC 1 cut(s) 1224
HspAI GCGC 3 cut(s) 88, 1299, 1388
KroI GCCGGC 1 cut(s) 941
KroNI GCCGGC 1 cut(s) 943
Ksp22I TGATCA 1 cut(s) 1545
KspI CCGCGG 1 cut(s) 639
Kzo9I GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
LguI GCTCTTC 3 cut(s) 467, 837, 1599
LmnI GCTCC 4 cut(s) 22, 296, 743, 1349
LweI GCATC 4 cut(s) 319, 787, 1252, 1345
MaeI CTAG 3 cut(s) 833, 875, 1587
MaeII ACGT 2 cut(s) 594, 753
MaeIII GTNAC 5 cut(s) 68, 341, 917, 1052, 1381
MalI GATC 9 cut(s) 173, 206, 229, 703, 1160, 1234, 1467, 1547, 1611
MbiI CCGCTC 1 cut(s) 184
MboI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
MhlI GDGCHC 5 cut(s) 294, 468, 626, 1594, 1623
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 4 cut(s) 31, 581, 777, 1128
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 6 cut(s) 257, 494, 716, 1064, 1412, 1573
Mox20I TGGCCA 1 cut(s) 750
MroNI GCCGGC 1 cut(s) 941
MscI TGGCCA 1 cut(s) 750
MseI TTAA 3 cut(s) 780, 1209, 1636
MslI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 5 cut(s) 384, 571, 638, 928, 1304
MvaI CCWGG 4 cut(s) 134, 734, 1108, 1366
MvnI CGCG 2 cut(s) 638, 1157
MwoI GCNNNNNNNGC 8 cut(s) 190, 641, 686, 743, 884, 976, 1212, 1237
NaeI GCCGGC 1 cut(s) 943
NciI CCSGG 6 cut(s) 57, 169, 225, 239, 294, 1150
NcoI CCATGG 1 cut(s) 1524
NdeII GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
NgoMIV GCCGGC 1 cut(s) 941
NheI GCTAGC 1 cut(s) 874
NlaIV GGNNCC 4 cut(s) 24, 336, 723, 1153
NmeAIII GCCGAG 1 cut(s) 1426
NmuCI GTSAC 4 cut(s) 341, 917, 1052, 1381
NsbI TGCGCA 2 cut(s) 89, 1300
NspI RCATGY 1 cut(s) 1319
PaeI GCATGC 1 cut(s) 1319
PaeR7I CTCGAG 3 cut(s) 185, 467, 1581
PciSI GCTCTTC 3 cut(s) 467, 837, 1599
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PdiI GCCGGC 1 cut(s) 943
PfeI GAWTC 6 cut(s) 142, 242, 509, 1307, 1432, 1483
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 2 cut(s) 167, 237
Ple19I CGATCG 1 cut(s) 1235
PleI GAGTC 6 cut(s) 257, 494, 715, 1064, 1411, 1573
PpsI GAGTC 6 cut(s) 257, 494, 715, 1064, 1411, 1573
PpuMI RGGWCCY 1 cut(s) 722
PsiI TTATAA 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 1594
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 4 cut(s) 132, 732, 1106, 1364
PspFI CCCAGC 1 cut(s) 1418
PspGI CCWGG 4 cut(s) 132, 732, 1106, 1364
PspN4I GGNNCC 4 cut(s) 24, 336, 723, 1153
PspPI GGNCC 5 cut(s) 4, 232, 334, 722, 1152
PspPPI RGGWCCY 1 cut(s) 722
PspXI VCTCGAGB 1 cut(s) 1581
PstI CTGCAG 1 cut(s) 1395
PstNI CAGNNNCTG 2 cut(s) 345, 1486
PvuI CGATCG 1 cut(s) 1235
PvuII CAGCTG 1 cut(s) 571
RgaI GCGATCGC 1 cut(s) 1235
RsaI GTAC 3 cut(s) 101, 959, 988
RsaNI GTAC 3 cut(s) 100, 958, 987
RseI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SacI GAGCTC 1 cut(s) 1594
SacII CCGCGG 1 cut(s) 639
SapI GCTCTTC 3 cut(s) 467, 837, 1599
SaqAI TTAA 3 cut(s) 780, 1209, 1636
Sau3AI GATC 9 cut(s) 171, 204, 227, 701, 1158, 1232, 1465, 1545, 1609
Sau96I GGNCC 5 cut(s) 4, 232, 334, 722, 1152
SchI GAGTC 6 cut(s) 257, 494, 716, 1064, 1412, 1573
SduI GDGCHC 5 cut(s) 294, 468, 626, 1594, 1623
SfaAI GCGATCGC 1 cut(s) 1235
SfaNI GCATC 4 cut(s) 319, 787, 1252, 1345
SfcI CTRYAG 1 cut(s) 1391
Sfr274I CTCGAG 3 cut(s) 185, 467, 1581
Sfr303I CCGCGG 1 cut(s) 639
SgfI GCGATCGC 1 cut(s) 1235
SgrBI CCGCGG 1 cut(s) 639
SinI GGWCC 3 cut(s) 4, 334, 722
SlaI CTCGAG 3 cut(s) 185, 467, 1581
SmiMI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SmlI CTYRAG 5 cut(s) 185, 467, 546, 629, 1581
SmoI CTYRAG 5 cut(s) 185, 467, 546, 629, 1581
SphI GCATGC 1 cut(s) 1319
Sse9I AATT 4 cut(s) 31, 581, 777, 1128
SspMI CTAG 3 cut(s) 833, 875, 1587
SstI GAGCTC 1 cut(s) 1594
StyI CCWWGG 2 cut(s) 1524, 1623
TaaI ACNGT 2 cut(s) 154, 860
TaiI ACGT 2 cut(s) 597, 756
TasI AATT 4 cut(s) 31, 581, 777, 1128
TatI WGTACW 2 cut(s) 99, 957
TauI GCSGC 6 cut(s) 638, 641, 941, 1043, 1106, 1157
TfiI GAWTC 6 cut(s) 142, 242, 509, 1307, 1432, 1483
Tru1I TTAA 3 cut(s) 780, 1209, 1636
Tru9I TTAA 3 cut(s) 780, 1209, 1636
TscAI CASTG 4 cut(s) 346, 700, 843, 1301
TseFI GTSAC 4 cut(s) 341, 917, 1052, 1381
Tsp45I GTSAC 4 cut(s) 341, 917, 1052, 1381
TspDTI ATGAA 5 cut(s) 306, 364, 391, 474, 1493
TspGWI ACGGA 3 cut(s) 61, 828, 1179
TspRI CASTG 4 cut(s) 346, 700, 843, 1301
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 4, 334, 722
XapI RAATTY 2 cut(s) 31, 581
XceI RCATGY 1 cut(s) 1319
XhoI CTCGAG 3 cut(s) 185, 467, 1581
XspI CTAG 3 cut(s) 833, 875, 1587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.