MD14G1005600.v1.1

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013599
Physical Location & Seq
Reverse (-)
615625 .. 617250
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1005600.v1.1.491

Sequence Viewer

Length: 1626 bp
ATGGACAGGCTCATAAGTTTGGAGCCATCAAACTTTGTTGCAATAAGGATCGAACCGGGACAGAAATGTTACGGCCAGCTCACTTTACGCAACGTTATGTACACTATGCCGGTAGCGTTCAGGCTCCAACCGCTGATCAAGAATCGTTACACGGTGAAGCCTCAGTCCGGGATCATATCTCCTCTCGAGAAGTTGACCGTAGAGATTGTTTATCACCTTCCACCGGGGTCCAGTCTCCCCGACTCCTTCCCTTACTGCAATGACTCCTTCCTTTTGCATAGCGTGGTTGTGCCCGGAGCAGCGATCAAAGACTCTTCTTCGACATTCGATGCTGTCCCAAACGACTGGTTCACCACAAAGAAGAAACAAGTGTTCATAGATAGTGGTGTCAAGATTATGTTTGTTGGTTCACCTGTTTTGGCACAACTTGTGTCCGATGGTTTGATGGATGATATTCGAGAAGTGCTTGAGAAGAGTGAAACTTCATGGAGAGCTGCCGATTCCGTGGATTCTGAGGGCCAATCTTTGCTTCACTTGGCTGTTGCTCAAGGACGGCCTGACCTAGTCCAGCTACTTCTTGAATTTGAGCCTGACGTGGAGGCTCAAAGCCGATCAGGGTCTACCCCACTCGAGGCTGCAGCCTCGAAAGGAGAAGCCTTGATAGTCGAACTTTTAATGGCCCGACGTGCCAGCACTGAACGATCTGAGTCCTCCACTTGGGGCCCCATTCACCTTGCGGCCGGAGGAGGCCACGTAGAGGTTCTGAGGCTTCTTATAATCAAGGGGGCTAATGTTGATGCGCTGACTAAAGATGGTAGCACAGCTTTGCACCTTGCGGTGGAGGAGCGAAGAAGGGACTGCGCAAGGCTCTTATTGGCGAGCGGGGCAAAAGCTGGAGTTCGTGATTGTAGAGATGGTGACACGCCTTTGCATATCGCAGCTAGCATGGGGGACGAGTACATGGTGAAGCTGTTGCTGCAAAAGGGTGCCAATAAAGATATCAGAAACTTTGCCGGCCTAACGGCCTATGATGTTGCGTTGGAGAATGGACACACGAGACTATTTGACGCTCTTCGACTTGGTGATAGCTTGTGCATTGCGGCGAGGAAGGGAGAGGTGAGAACAATTGTAAGGCTGCTTGAGACAGGTGCGACGATCAATGGAAGAGACCAACATGGGTGGACTGCTCTCCATAGGGCATGTTTCAAAGGGAACATTGAAGTCGTTCGAACGCTGCTCGAAAAGGGTGTGGATGTGGATGCTAAAGATGAGGATGGATACACCGCGTTGCACTGCGCAGTGGAGTCAGGACATGCGGATGTGATTGAGATGTTGGTAAAGAAAGGAGCTGATGCTGAAGCTCGAACTAACAAAGGTGTGACCGCACTGAAGATTGCTGAGTCTTTGCACTACGCTGGGATTACTAGGATACTTATTCATGGAGGTGCAACAAAAGCCAATAGTAATATGGCACGTATCTCGACCCAGACCTCAGTTGCATTTGGGAAAAAATCTAAGGGACTAGAGGAGGAGATCATCAAAGGAGGGATGAAGAAGAAGAAATCGAGTCGTGCTAGAGCTCTTCGTGGGAGCTTCGACCATTCGATCCCATTGGTTGTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

542

Amino Acids

58.62

Weight (kDa)

8.06

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 5 - 108 1.9e-07 MSP (Major sperm protein) domain
Ank_2 PF12796 166 - 230 3.9e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 168 - 211 4.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 239 - 302 1.6e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 243 - 292 1.3e-08 Ankyrin repeats (many copies)
Ank_4 PF13637 280 - 326 1.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 295 - 358 2.1e-08 Ankyrin repeats (3 copies)
Ank PF00023 305 - 336 3.6e-09 Ankyrin repeat
Ank_4 PF13637 364 - 413 2.3e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 364 - 423 1.1e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 379 - 431 6e-09 Ankyrin repeats (many copies)
Ank PF00023 392 - 423 6.4e-09 Ankyrin repeat
Ank_3 PF13606 392 - 421 1e-06 Ankyrin repeat
Ank_4 PF13637 400 - 446 4.3e-13 Ankyrin repeats (many copies)
Ank_5 PF13857 412 - 466 3.6e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 418 - 483 5.6e-10 Ankyrin repeats (3 copies)
Ank PF00023 425 - 457 3.5e-08 Ankyrin repeat
Ank_3 PF13606 425 - 453 1e-05 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
Acc16I TGCGCA 2 cut(s) 862, 1297
AccB1I GGYRCC 1 cut(s) 986
AccBSI CCGCTC 1 cut(s) 882
AccI GTMKAC 1 cut(s) 620
AccII CGCG 1 cut(s) 1286
AciI CCGC 8 cut(s) 131, 737, 836, 882, 1100, 1284, 1316, 1383
AclI AACGTT 1 cut(s) 93
AclWI GGATC 3 cut(s) 56, 179, 1600
AcoI YGGCCR 2 cut(s) 73, 738
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 2 cut(s) 1377, 1409
AfaI GTAC 2 cut(s) 101, 959
AfiI CCNNNNNNNGG 6 cut(s) 167, 223, 631, 717, 757, 838
AgsI TTSAA 3 cut(s) 581, 1207, 1220
AjiI CACGTC 2 cut(s) 595, 686
AjuI GAANNNNNNNTTGG 2 cut(s) 513, 545
Alw21I GWGCWC 1 cut(s) 1582
Alw26I GTCTC 4 cut(s) 239, 1051, 1136, 1161
AlwI GGATC 3 cut(s) 56, 179, 1600
Ama87I CYCGRG 2 cut(s) 185, 629
AoxI GGCC 9 cut(s) 73, 517, 554, 678, 721, 738, 748, 1015, 1023
ApaI GGGCCC 1 cut(s) 725
ApeKI GCWGC 8 cut(s) 299, 494, 635, 638, 938, 976, 1135, 1234
ApoI RAATTY 1 cut(s) 581
Asp700I GAANNNNTTC 1 cut(s) 1224
AspLEI GCGC 3 cut(s) 802, 863, 1298
AspS9I GGNCC 5 cut(s) 228, 517, 679, 721, 722
AsuC2I CCSGG 4 cut(s) 57, 169, 225, 294
AsuHPI GGTGA 9 cut(s) 166, 206, 343, 402, 722, 929, 976, 1094, 1129
AsuII TTCGAA 1 cut(s) 1228
AsuNHI GCTAGC 1 cut(s) 941
AvaI CYCGRG 2 cut(s) 185, 629
AvaII GGWCC 1 cut(s) 228
BaeGI GKGCMC 2 cut(s) 294, 725
BanI GGYRCC 1 cut(s) 986
BanII GRGCYC 2 cut(s) 725, 1582
BauI CACGAG 1 cut(s) 1054
Bbv12I GWGCWC 1 cut(s) 1582
BbvI GCAGC 8 cut(s) 311, 481, 622, 650, 950, 963, 1122, 1221
BccI CCATC 6 cut(s) 34, 431, 439, 806, 908, 1268
BceAI ACGGC 3 cut(s) 88, 569, 1038
BcgI CGANNNNNNTGC 2 cut(s) 1461, 1495
BciVI GTATCC 2 cut(s) 1271, 1422
BclI TGATCA 1 cut(s) 135
BcnI CCSGG 4 cut(s) 57, 169, 225, 294
BcoDI GTCTC 4 cut(s) 239, 1051, 1136, 1161
BfaI CTAG 5 cut(s) 563, 942, 1425, 1523, 1575
BfmI CTRYAG 1 cut(s) 636
BfuI GTATCC 2 cut(s) 1271, 1422
Bme1390I CCNGG 4 cut(s) 57, 169, 225, 294
Bme18I GGWCC 1 cut(s) 228
BmeT110I CYCGRG 2 cut(s) 185, 629
BmgBI CACGTC 2 cut(s) 595, 686
BmgT120I GGNCC 5 cut(s) 228, 517, 679, 721, 722
BmiI GGNNCC 7 cut(s) 24, 125, 229, 722, 723, 724, 988
BmrFI CCNGG 4 cut(s) 57, 169, 225, 294
BmsI GCATC 4 cut(s) 319, 787, 1249, 1342
BmtI GCTAGC 1 cut(s) 945
BpmI CTGGAG 1 cut(s) 915
Bpu14I TTCGAA 1 cut(s) 1228
BpuEI CTTGAG 3 cut(s) 488, 531, 1160
BpuMI CCSGG 4 cut(s) 57, 169, 225, 294
BsaAI YACGTR 2 cut(s) 754, 1475
BsaI GGTCTC 1 cut(s) 1161
BsaJI CCNNGG 2 cut(s) 224, 504
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 6 cut(s) 167, 223, 631, 717, 757, 838
Bse118I RCCGGY 2 cut(s) 109, 1013
Bse1I ACTGG 2 cut(s) 231, 350
Bse3DI GCAATG 2 cut(s) 265, 1095
BseDI CCNNGG 2 cut(s) 224, 504
BseGI GGATG 6 cut(s) 454, 1258, 1264, 1279, 1324, 1554
BseLI CCNNNNNNNGG 6 cut(s) 167, 223, 631, 717, 757, 838
BseMI GCAATG 2 cut(s) 265, 1095
BseMII CTCAG 6 cut(s) 176, 504, 696, 755, 1389, 1506
BseNI ACTGG 2 cut(s) 231, 350
BseRI GAGGAG 5 cut(s) 171, 759, 857, 1541, 1544
BseSI GKGCMC 2 cut(s) 294, 725
BseX3I CGGCCG 1 cut(s) 738
BseXI GCAGC 8 cut(s) 311, 481, 622, 650, 950, 963, 1122, 1221
BseYI CCCAGC 1 cut(s) 1415
Bsh1236I CGCG 1 cut(s) 1286
Bsh1285I CGRYCG 1 cut(s) 741
BshFI GGCC 9 cut(s) 75, 519, 556, 680, 723, 740, 750, 1017, 1025
BshNI GGYRCC 1 cut(s) 986
BsiEI CGRYCG 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 1582
BsiHKCI CYCGRG 2 cut(s) 185, 629
BsiSI CCGG 7 cut(s) 56, 110, 168, 224, 294, 741, 1014
BslFI GGGAC 5 cut(s) 72, 320, 869, 965, 1533
BslI CCNNNNNNNGG 6 cut(s) 167, 223, 631, 717, 757, 838
BsmAI GTCTC 4 cut(s) 239, 1051, 1136, 1161
BsmFI GGGAC 5 cut(s) 72, 320, 869, 965, 1533
BsnI GGCC 9 cut(s) 75, 519, 556, 680, 723, 740, 750, 1017, 1025
Bso31I GGTCTC 1 cut(s) 1161
BsoBI CYCGRG 2 cut(s) 185, 629
Bsp119I TTCGAA 1 cut(s) 1228
Bsp120I GGGCCC 1 cut(s) 721
Bsp1286I GDGCHC 3 cut(s) 294, 725, 1582
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
BspACI CCGC 8 cut(s) 131, 737, 836, 882, 1100, 1284, 1316, 1383
BspANI GGCC 9 cut(s) 75, 519, 556, 680, 723, 740, 750, 1017, 1025
BspCNI CTCAG 6 cut(s) 175, 505, 697, 756, 1390, 1505
BspFNI CGCG 1 cut(s) 1286
BspLI GGNNCC 7 cut(s) 24, 125, 229, 722, 723, 724, 988
BspMAI CTGCAG 1 cut(s) 640
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 3 cut(s) 56, 179, 1600
BspQI GCTCTTC 2 cut(s) 1077, 1587
BspT104I TTCGAA 1 cut(s) 1228
BspT107I GGYRCC 1 cut(s) 986
BspTNI GGTCTC 1 cut(s) 1161
BsrBI CCGCTC 1 cut(s) 882
BsrDI GCAATG 2 cut(s) 265, 1095
BsrFI RCCGGY 2 cut(s) 109, 1013
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 2 cut(s) 231, 350
BssAI RCCGGY 2 cut(s) 109, 1013
BssECI CCNNGG 2 cut(s) 224, 504
BssMI GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
BssSI CACGAG 1 cut(s) 1054
Bst2BI CACGAG 1 cut(s) 1054
Bst4CI ACNGT 2 cut(s) 154, 199
Bst6I CTCTTC 5 cut(s) 319, 467, 1077, 1159, 1587
BstAUI TGTACA 1 cut(s) 99
BstBAI YACGTR 2 cut(s) 754, 1475
BstBI TTCGAA 1 cut(s) 1228
BstC8I GCNNGC 5 cut(s) 77, 691, 880, 943, 1015
BstDEI CTNAG 7 cut(s) 162, 513, 705, 764, 1398, 1492, 1515
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 6 cut(s) 454, 1258, 1264, 1279, 1324, 1554
BstFNI CGCG 1 cut(s) 1286
BstHHI GCGC 3 cut(s) 802, 863, 1298
BstKTI GATC 9 cut(s) 51, 138, 174, 306, 614, 704, 1158, 1536, 1608
BstMAI GTCTC 4 cut(s) 239, 1051, 1136, 1161
BstMBI GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
BstMCI CGRYCG 1 cut(s) 741
BstMWI GCNNNNNNNGC 5 cut(s) 130, 686, 884, 976, 1454
BstNSI RCATGY 2 cut(s) 1203, 1316
BstSCI CCNGG 4 cut(s) 55, 167, 223, 292
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 2 cut(s) 294, 725
BstUI CGCG 1 cut(s) 1286
BstV1I GCAGC 8 cut(s) 311, 481, 622, 650, 950, 963, 1122, 1221
BstXI CCANNNNNNTGG 1 cut(s) 345
BstZI CGGCCG 1 cut(s) 738
BsuI GTATCC 2 cut(s) 1271, 1422
BsuRI GGCC 9 cut(s) 75, 519, 556, 680, 723, 740, 750, 1017, 1025
BtgI CCRYGG 1 cut(s) 504
BtrI CACGTC 2 cut(s) 595, 686
BtsCI GGATG 6 cut(s) 454, 1258, 1264, 1279, 1324, 1554
BtsI GCAGTG 2 cut(s) 1291, 1305
BtsIMutI CAGTG 4 cut(s) 693, 1291, 1305, 1385
Cac8I GCNNGC 5 cut(s) 77, 691, 880, 943, 1015
CfoI GCGC 3 cut(s) 802, 863, 1298
Cfr10I RCCGGY 2 cut(s) 109, 1013
Cfr13I GGNCC 5 cut(s) 228, 517, 679, 721, 722
CseI GACGC 1 cut(s) 1076
Csp6I GTAC 2 cut(s) 100, 958
CspCI CAANNNNNGTGG 2 cut(s) 1160, 1195
CviAII CATG 7 cut(s) 486, 946, 961, 1175, 1200, 1313, 1439
CviQI GTAC 2 cut(s) 100, 958
DdeI CTNAG 7 cut(s) 162, 513, 705, 764, 1398, 1492, 1515
DpnI GATC 9 cut(s) 50, 137, 173, 305, 613, 703, 1157, 1535, 1607
DpnII GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
EaeI YGGCCR 2 cut(s) 73, 738
EagI CGGCCG 1 cut(s) 738
Eam1104I CTCTTC 5 cut(s) 319, 467, 1077, 1159, 1587
EarI CTCTTC 5 cut(s) 319, 467, 1077, 1159, 1587
Ecl136II GAGCTC 1 cut(s) 1580
EclXI CGGCCG 1 cut(s) 738
Eco24I GRGCYC 2 cut(s) 725, 1582
Eco31I GGTCTC 1 cut(s) 1161
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 1 cut(s) 228
Eco52I CGGCCG 1 cut(s) 738
Eco53kI GAGCTC 1 cut(s) 1580
Eco57I CTGAAG 2 cut(s) 1377, 1409
Eco88I CYCGRG 2 cut(s) 185, 629
EcoICRI GAGCTC 1 cut(s) 1580
EcoO109I RGGNCCY 2 cut(s) 721, 722
EcoRV GATATC 1 cut(s) 1000
EcoT38I GRGCYC 2 cut(s) 725, 1582
FaeI CATG 7 cut(s) 489, 949, 964, 1178, 1203, 1316, 1442
FaqI GGGAC 5 cut(s) 72, 320, 869, 965, 1533
FatI CATG 7 cut(s) 485, 945, 960, 1174, 1199, 1312, 1438
FauI CCCGC 1 cut(s) 875
FbaI TGATCA 1 cut(s) 135
FblI GTMKAC 1 cut(s) 620
FokI GGATG 6 cut(s) 461, 1265, 1271, 1286, 1331, 1561
FriOI GRGCYC 2 cut(s) 725, 1582
FspBI CTAG 5 cut(s) 563, 942, 1425, 1523, 1575
FspI TGCGCA 2 cut(s) 862, 1297
GlaI GCGC 3 cut(s) 801, 862, 1297
GsaI CCCAGC 1 cut(s) 1419
GsuI CTGGAG 1 cut(s) 915
HaeIII GGCC 9 cut(s) 75, 519, 556, 680, 723, 740, 750, 1017, 1025
HapII CCGG 7 cut(s) 56, 110, 168, 224, 294, 741, 1014
HgaI GACGC 1 cut(s) 1076
HhaI GCGC 3 cut(s) 802, 863, 1298
Hin1II CATG 7 cut(s) 489, 949, 964, 1178, 1203, 1316, 1442
Hin6I GCGC 3 cut(s) 800, 861, 1296
HinP1I GCGC 3 cut(s) 800, 861, 1296
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HpaII CCGG 7 cut(s) 56, 110, 168, 224, 294, 741, 1014
HphI GGTGA 9 cut(s) 166, 206, 343, 402, 722, 929, 976, 1094, 1129
Hpy166II GTNNAC 6 cut(s) 102, 195, 351, 410, 621, 1182
Hpy188I TCNGA 5 cut(s) 436, 514, 706, 765, 1004
Hpy188III TCNNGA 9 cut(s) 139, 185, 187, 391, 458, 578, 902, 1308, 1480
Hpy8I GTNNAC 6 cut(s) 102, 195, 351, 410, 621, 1182
Hpy99I CGWCG 2 cut(s) 687, 1156
HpyAV CCTTC 5 cut(s) 227, 256, 277, 846, 1102
HpyCH4III ACNGT 2 cut(s) 154, 199
HpyCH4IV ACGT 5 cut(s) 93, 594, 685, 753, 1474
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 686, 884, 976, 1454
HpyF3I CTNAG 7 cut(s) 162, 513, 705, 764, 1398, 1492, 1515
HpySE526I ACGT 5 cut(s) 93, 594, 685, 753, 1474
Hsp92II CATG 7 cut(s) 489, 949, 964, 1178, 1203, 1316, 1442
HspAI GCGC 3 cut(s) 800, 861, 1296
KroI GCCGGC 1 cut(s) 1013
KroNI GCCGGC 1 cut(s) 1015
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
LguI GCTCTTC 2 cut(s) 1077, 1587
LmnI GCTCC 6 cut(s) 22, 129, 296, 844, 1346, 1590
Lsp1109I GCAGC 8 cut(s) 311, 481, 622, 650, 950, 963, 1122, 1221
LweI GCATC 4 cut(s) 319, 787, 1249, 1342
MaeI CTAG 5 cut(s) 563, 942, 1425, 1523, 1575
MaeII ACGT 5 cut(s) 93, 594, 685, 753, 1474
MaeIII GTNAC 4 cut(s) 68, 146, 917, 1378
MalI GATC 9 cut(s) 50, 137, 173, 305, 613, 703, 1157, 1535, 1607
MbiI CCGCTC 1 cut(s) 882
MboI GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
MfeI CAATTG 1 cut(s) 1125
MhlI GDGCHC 3 cut(s) 294, 725, 1582
MluCI AATT 2 cut(s) 581, 1125
MlyI GAGTC 7 cut(s) 236, 257, 305, 716, 1313, 1409, 1576
MmeI TCCRAC 2 cut(s) 151, 1020
MroNI GCCGGC 1 cut(s) 1013
MroXI GAANNNNTTC 1 cut(s) 1224
MseI TTAA 1 cut(s) 674
MslI CAYNNNNRTG 2 cut(s) 1317, 1443
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 7 cut(s) 56, 110, 168, 224, 294, 741, 1014
MspR9I CCNGG 4 cut(s) 57, 169, 225, 294
MunI CAATTG 1 cut(s) 1125
MvnI CGCG 1 cut(s) 1286
MwoI GCNNNNNNNGC 5 cut(s) 130, 686, 884, 976, 1454
NaeI GCCGGC 1 cut(s) 1015
NciI CCSGG 4 cut(s) 57, 169, 225, 294
NdeII GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
NgoMIV GCCGGC 1 cut(s) 1013
NheI GCTAGC 1 cut(s) 941
NlaIII CATG 7 cut(s) 489, 949, 964, 1178, 1203, 1316, 1442
NlaIV GGNNCC 7 cut(s) 24, 125, 229, 722, 723, 724, 988
NmuCI GTSAC 2 cut(s) 917, 1378
NsbI TGCGCA 2 cut(s) 862, 1297
NspI RCATGY 2 cut(s) 1203, 1316
NspV TTCGAA 1 cut(s) 1228
PaeR7I CTCGAG 2 cut(s) 185, 629
PciSI GCTCTTC 2 cut(s) 1077, 1587
PdiI GCCGGC 1 cut(s) 1015
PdmI GAANNNNTTC 1 cut(s) 1224
PfeI GAWTC 3 cut(s) 142, 500, 509
PflFI GACNNNGTC 1 cut(s) 563
PfoI TCCNGGA 1 cut(s) 167
PleI GAGTC 7 cut(s) 236, 257, 305, 715, 1312, 1408, 1575
PpsI GAGTC 7 cut(s) 236, 257, 305, 715, 1312, 1408, 1575
Ppu21I YACGTR 2 cut(s) 754, 1475
PsiI TTATAA 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 1582
Psp1406I AACGTT 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 1415
PspN4I GGNNCC 7 cut(s) 24, 125, 229, 722, 723, 724, 988
PspOMI GGGCCC 1 cut(s) 721
PspPI GGNCC 5 cut(s) 228, 517, 679, 721, 722
PspXI VCTCGAGB 1 cut(s) 629
PstI CTGCAG 1 cut(s) 640
PsyI GACNNNGTC 1 cut(s) 563
RsaI GTAC 2 cut(s) 101, 959
RsaNI GTAC 2 cut(s) 100, 958
RseI CAYNNNNRTG 2 cut(s) 1317, 1443
SacI GAGCTC 1 cut(s) 1582
SapI GCTCTTC 2 cut(s) 1077, 1587
SaqAI TTAA 1 cut(s) 674
Sau3AI GATC 9 cut(s) 48, 135, 171, 303, 611, 701, 1155, 1533, 1605
Sau96I GGNCC 5 cut(s) 228, 517, 679, 721, 722
SchI GAGTC 7 cut(s) 236, 257, 305, 716, 1313, 1409, 1576
ScrFI CCNGG 4 cut(s) 57, 169, 225, 294
SduI GDGCHC 3 cut(s) 294, 725, 1582
SfaNI GCATC 4 cut(s) 319, 787, 1249, 1342
SfcI CTRYAG 1 cut(s) 636
Sfr274I CTCGAG 2 cut(s) 185, 629
SfuI TTCGAA 1 cut(s) 1228
SinI GGWCC 1 cut(s) 228
SlaI CTCGAG 2 cut(s) 185, 629
SmiMI CAYNNNNRTG 2 cut(s) 1317, 1443
SmlI CTYRAG 5 cut(s) 185, 467, 546, 629, 1139
SmoI CTYRAG 5 cut(s) 185, 467, 546, 629, 1139
Sse9I AATT 2 cut(s) 581, 1125
SsiI CCGC 8 cut(s) 131, 737, 836, 882, 1100, 1284, 1316, 1383
SspMI CTAG 5 cut(s) 563, 942, 1425, 1523, 1575
SstI GAGCTC 1 cut(s) 1582
StyD4I CCNGG 4 cut(s) 55, 167, 223, 292
TaaI ACNGT 2 cut(s) 154, 199
TaiI ACGT 5 cut(s) 96, 597, 688, 756, 1477
TasI AATT 2 cut(s) 581, 1125
TatI WGTACW 2 cut(s) 99, 957
TauI GCSGC 2 cut(s) 740, 1103
TfiI GAWTC 3 cut(s) 142, 500, 509
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 4 cut(s) 700, 1298, 1305, 1392
TseFI GTSAC 2 cut(s) 917, 1378
TseI GCWGC 8 cut(s) 299, 494, 635, 638, 938, 976, 1135, 1234
Tsp45I GTSAC 2 cut(s) 917, 1378
TspDTI ATGAA 4 cut(s) 364, 474, 1427, 1565
TspGWI ACGGA 1 cut(s) 493
TspRI CASTG 4 cut(s) 700, 1298, 1305, 1392
Tth111I GACNNNGTC 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 228
XapI RAATTY 1 cut(s) 581
XceI RCATGY 2 cut(s) 1203, 1316
XcmI CCANNNNNNNNNTGG 1 cut(s) 1465
XhoI CTCGAG 2 cut(s) 185, 629
XmiI GTMKAC 1 cut(s) 620
XmnI GAANNNNTTC 1 cut(s) 1224
XspI CTAG 5 cut(s) 563, 942, 1425, 1523, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.