Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014592

Physical Location & Seq

Genomic Coordinates Forward (+)

34305717 .. 34307882

Gene Structure 2166 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1255800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGCCAACAATACTCAAAACCAGCTTTTTCCTTAAGCACTTTTCCTACTGTAGCGTTTCGCGTTGTAAGATCCACTGCTCATATCTGTCCACTCAAGCTTGTTCAAAACCCCAAAGCGTGTCCAAAAGCAGAAAAGCCAGCAGCTTTCTTGTTTACTGTAATTCCCAGATCACCCAAAACGGAAGAAATGGTGATATCAAACAAGCCGAATCAATATTCAATCGCATGCCCGAGAAAAACACTATTTCCTGGACTGCTATGCTCACTGCATATGCCGAAAACGGCCAAACTTCCAAAGCACGTAAACTGTTCGATGAAATTCCCCAACGAAACATAGCATCGTATAATGCGATGATCACGGCTTATATTAGAAATAAGTATATGGTAGGTGAAGCTTTTGAGTTGTTTTCTGGGATGCCGGAGCGCAATGAGGTGTCTTATAGTGCCATGATCACGGGTTTTGTAAAGGCGGGGATGTTTGACAAGGCTGAGAAGCTTTACTGCGAAATGCCAGTTAGGTGGCGGGAACCAGTTTGTTCAAATGTTTTGATAAATGGGTATTTGAAGGTTGGAAGAACTGAAGACGCAGTTCGAGTTTTTGAGGGTATGATAAATAGCAATGTGGTTTCTCAGAGCTGTATGGTTGATGGGTATTGCAAAATGGGAAGGATTATTGATGCTAGAAATTTGTTTGATCAGATGCTGGAGAGGAATGTGGTTACTTGGACAGCACTGATTGATGGCTATATGAAGATGGGAATTTTTGAAGCCGGGTTTGAATTGTTCTTGGATATGAGTAGGGAAGGGCTAGTGAAGGTCAATAGCACTACAATGGCTGTACTGTTTGAAGCTTGTGGCAGCTTTGATAGATATCAAGAAGGGATTCAGATGCACGGGTTAGTTTCTCGTAAGGGGTTTGACTATGATGTCTTTTTAGGAAATTCTGTTATCATTATGTATTGTAGATTTGGATGTATGAATGAAGCTTCTAAGGTATTTAATATGATGAACAAGAAAGATGTAGTTTCCTGGAATTCCTTAATTGCGGGTTATGTTCAATGTGCTGAAACTGAAGAAGCTTTTAGATTGTTTGAGGTGATGCCTGCAAAGGATATATTCTCGTGGACGACCATGTTATCGGGATTCTCCTGCAAAGGGATGACTGAGAAAGCCATGGAGTTGTTCAAAATGATGCCTGAGAAGGATGATGTTGCTTGGACTGCCGTAATTTCGGGTTTTGTAAATAATGGGCAATATGAAGAGGCTCTCATTTGGTTTATTCAGATGCGTCGAGAGGAAATCAGGATCAATCCCCTTACTTTGAGCAGTGCACTCAGTGCTTCTGCCAGTTTGGCCAGTATAAACGAGGGGATGCAGATCCATGCTCTTTCATTTAAGATGGATATGGAGTTTGAATTATCCGTTCAAAATTCTCTGGTCTCTATGTATTCAAAGTGTGGGAACACTGTTGATGCCAATCATATCTTCAGAAGTATCACTTCGCCCAATACTGTTTCATTCAACTCATTGATTACAGGGTTTGCGCAAAATGGGTTTGGGAAAGAAGCTCTTAACTTATTTCGGAGCATGCAGAATGAAGATTGCGAGCCAAACCAAATCACCTTTCTGGGTGTTCTTTCAGCCTGCGTCCACATGGGACTAGTAGAAGAAGGATGGCAGTATTTTAATTCGATGAAATTGGAACACAATATAGAACCAGGACCGGATCACTACGCTTGCATGGTTGATCTCCTTGGTCGAGCTGGTTTGCTACATGAAGCTGTCGATTTGATTCATTCAATGCCGTTTGAGCCCCATACCGGGGTTTGGGGAGCTCTTCTTAGTGCTAGCAGGACTCATTTATGTCTTGATCTTGCACAGCTTGCAACTCGAAAGCTAATCAGATTGGAGCCTGATGACGCGACCCCTTATGTGGTCTTGTCTAATTTATATTCTACTTTAGGGAAAATGAAGGATGGAAACCAAGTGATGATGACCAAAAAATCAAGGGGGATAAGGAAGAGTCCAGGCTGCAGCTGGATTACAGTGAAGGACAAGGTTCATTTGTTTCTTGCAGGAGATCAATCCCATTTGGACTCGCAAGCAATTAAAGGATTACTCTCGATCATATCAATGGAAATGGGACAGCTACATTTTTATAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

722

Amino Acids

81.22

Weight (kDa)

6.74

Isoelectric Point (pI)

38.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	143 - 173	4.9e-06	PPR repeat family
PPR	PF01535	145 - 171	1.7e-07	PPR repeat
PPR_2	PF13041	237 - 285	3e-09	PPR repeat family
PPR_2	PF13041	339 - 370	1.9e-06	PPR repeat family
PPR	PF01535	373 - 401	2.4e-07	PPR repeat
PPR_2	PF13041	402 - 447	4.3e-07	PPR repeat family
PPR	PF01535	404 - 433	4.1e-06	PPR repeat
PPR_1	PF12854	501 - 530	6.1e-06	PPR repeat
PPR_2	PF13041	502 - 550	2e-11	PPR repeat family
PPR_3	PF13812	502 - 551	2.9e-06	Pentatricopeptide repeat domain
PPR	PF01535	505 - 535	6e-07	PPR repeat
E_motif	PF20431	623 - 681	3.8e-13	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014592)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53600 AT1G53600
fragaria_vesca	FvH4_3g16920
malus_domestica	MD03G1255800.v1.1
prunus_persica	Prupe.4G151400_v2.0.a1
pyrus_communis	pycom03g20240
rosa_chinensis	RchiOBHm_Chr5g0028141
rosa_laevigata	RLG00000033071
rosa_multiflora	Rmu_co8381305.1_g000001 Rmu_sc0002598.1_g000012
rosa_roxburghii	Rroxscaffold_1G00051540
rosa_rugosa	Rorug05G0104400
rosa_samantha	Rh5AG197600 Rh5BG196200 Rh5CG216200 Rh5DG197900
rosa_wichuraiana	Rw5G018060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	926
Acc16I	TGCGCA	1 cut(s)	1545
AccII	CGCG	2 cut(s)	61, 1922
AciI	CCGC	3 cut(s)	470, 523, 1046
AclWI	GGATC	4 cut(s)	64, 1313, 1372, 1734
AcoI	YGGCCR	2 cut(s)	283, 1353
AcsI	RAATTY	6 cut(s)	318, 685, 759, 940, 1033, 1429
AcuI	CTGAAG	3 cut(s)	600, 1092, 1471
AdeI	CACNNNGTG	1 cut(s)	1337
AfaI	GTAC	1 cut(s)	840
AfiI	CCNNNNNNNGG	6 cut(s)	1155, 1820, 1821, 1822, 1827, 1933
AflII	CTTAAG	1 cut(s)	32
AhlI	ACTAGT	1 cut(s)	1660
AjnI	CCWGG	4 cut(s)	248, 1028, 1717, 2026
AjuI	GAANNNNNNNTTGG	4 cut(s)	1469, 1501, 1608, 1640
Alw21I	GWGCWC	2 cut(s)	1333, 1837
Alw26I	GTCTC	1 cut(s)	1444
Alw44I	GTGCAC	1 cut(s)	1329
AlwI	GGATC	4 cut(s)	64, 1313, 1372, 1734
AlwNI	CAGNNNCTG	1 cut(s)	703
Ama87I	CYCGRG	1 cut(s)	230
AoxI	GGCC	2 cut(s)	283, 1353
ApaLI	GTGCAC	1 cut(s)	1329
ApeKI	GCWGC	4 cut(s)	141, 858, 2031, 2034
ApoI	RAATTY	6 cut(s)	318, 685, 759, 940, 1033, 1429
Asp700I	GAANNNNTTC	1 cut(s)	882
AspLEI	GCGC	2 cut(s)	426, 1546
AspS9I	GGNCC	1 cut(s)	1721
AsuC2I	CCSGG	2 cut(s)	772, 1822
AsuHPI	GGTGA	5 cut(s)	163, 203, 401, 1108, 1612
AsuNHI	GCTAGC	1 cut(s)	1847
AvaI	CYCGRG	1 cut(s)	230
AvaII	GGWCC	1 cut(s)	1721
BaeGI	GKGCMC	1 cut(s)	1333
BalI	TGGCCA	1 cut(s)	1355
BanII	GRGCYC	2 cut(s)	1815, 1837
BauI	CACGAG	1 cut(s)	1120
BbsI	GAAGAC	1 cut(s)	588
Bbv12I	GWGCWC	2 cut(s)	1333, 1837
BbvI	GCAGC	4 cut(s)	153, 870, 2018, 2046
BccI	CCATC	6 cut(s)	641, 734, 748, 1393, 1668, 1970
BceAI	ACGGC	4 cut(s)	298, 375, 1208, 1789
BciT130I	CCWGG	4 cut(s)	250, 1030, 1719, 2028
BclI	TGATCA	3 cut(s)	354, 450, 694
BcnI	CCSGG	2 cut(s)	772, 1822
BcoDI	GTCTC	1 cut(s)	1444
BcuI	ACTAGT	1 cut(s)	1660
BfaI	CTAG	4 cut(s)	681, 809, 1661, 1848
BfmI	CTRYAG	2 cut(s)	49, 2032
BfrI	CTTAAG	1 cut(s)	32
BglI	GCCNNNNNGGC	1 cut(s)	1352
BisI	GCNGC	4 cut(s)	142, 859, 2032, 2035
BlsI	GCNGC	4 cut(s)	143, 860, 2033, 2036
Bme1390I	CCNGG	6 cut(s)	250, 772, 1030, 1719, 1822, 2028
Bme18I	GGWCC	1 cut(s)	1721
BmeT110I	CYCGRG	1 cut(s)	230
BmgT120I	GGNCC	1 cut(s)	1721
BmiI	GGNNCC	2 cut(s)	528, 1911
BmrFI	CCNGG	6 cut(s)	250, 772, 1030, 1719, 1822, 2028
BmtI	GCTAGC	1 cut(s)	1851
BpiI	GAAGAC	1 cut(s)	588
BpmI	CTGGAG	1 cut(s)	725
BpuEI	CTTGAG	1 cut(s)	78
BpuMI	CCSGG	2 cut(s)	772, 1822
BsaAI	YACGTR	1 cut(s)	302
BsaBI	GATNNNNATC	3 cut(s)	870, 1376, 1476
BsaI	GGTCTC	1 cut(s)	1444
BsaJI	CCNNGG	3 cut(s)	1173, 1753, 1821
BsaWI	WCCGGW	1 cut(s)	1723
Bsc4I	CCNNNNNNNGG	6 cut(s)	1155, 1820, 1821, 1822, 1827, 1933
Bse1I	ACTGG	4 cut(s)	512, 530, 1347, 1356
Bse3DI	GCAATG	2 cut(s)	433, 625
Bse8I	GATNNNNATC	3 cut(s)	870, 1376, 1476
BseBI	CCWGG	4 cut(s)	250, 1030, 1719, 2028
BseDI	CCNNGG	3 cut(s)	1173, 1753, 1821
BseGI	GGATG	8 cut(s)	420, 480, 977, 1164, 1210, 1377, 1679, 1981
BseJI	GATNNNNATC	3 cut(s)	870, 1376, 1476
BseLI	CCNNNNNNNGG	6 cut(s)	1155, 1820, 1821, 1822, 1827, 1933
BseMI	GCAATG	2 cut(s)	433, 625
BseMII	CTCAG	5 cut(s)	480, 644, 1155, 1188, 1348
BseNI	ACTGG	4 cut(s)	512, 530, 1347, 1356
BseSI	GKGCMC	1 cut(s)	1333
BseXI	GCAGC	4 cut(s)	153, 870, 2018, 2046
Bsh1236I	CGCG	2 cut(s)	61, 1922
BshFI	GGCC	2 cut(s)	285, 1355
BsiHKAI	GWGCWC	2 cut(s)	1333, 1837
BsiHKCI	CYCGRG	1 cut(s)	230
BsiSI	CCGG	4 cut(s)	419, 771, 1724, 1821
BslFI	GGGAC	2 cut(s)	1671, 2157
BslI	CCNNNNNNNGG	6 cut(s)	1155, 1820, 1821, 1822, 1827, 1933
BsmAI	GTCTC	1 cut(s)	1444
BsmFI	GGGAC	2 cut(s)	1671, 2157
BsnI	GGCC	2 cut(s)	285, 1355
Bso31I	GGTCTC	1 cut(s)	1444
BsoBI	CYCGRG	1 cut(s)	230
Bsp1286I	GDGCHC	3 cut(s)	1333, 1815, 1837
Bsp19I	CCATGG	1 cut(s)	1173
BspACI	CCGC	3 cut(s)	470, 523, 1046
BspANI	GGCC	2 cut(s)	285, 1355
BspCNI	CTCAG	5 cut(s)	481, 643, 1156, 1189, 1347
BspFNI	CGCG	2 cut(s)	61, 1922
BspLI	GGNNCC	2 cut(s)	528, 1911
BspMAI	CTGCAG	1 cut(s)	2036
BspOI	GCTAGC	1 cut(s)	1851
BspPI	GGATC	4 cut(s)	64, 1313, 1372, 1734
BspQI	GCTCTTC	1 cut(s)	1842
BspTI	CTTAAG	1 cut(s)	32
BspTNI	GGTCTC	1 cut(s)	1444
BsrDI	GCAATG	2 cut(s)	433, 625
BsrI	ACTGG	4 cut(s)	512, 530, 1347, 1356
BssECI	CCNNGG	3 cut(s)	1173, 1753, 1821
BssSI	CACGAG	1 cut(s)	1120
BssT1I	CCWWGG	2 cut(s)	1173, 1753
Bst2BI	CACGAG	1 cut(s)	1120
Bst2UI	CCWGG	4 cut(s)	250, 1030, 1719, 2028
Bst4CI	ACNGT	7 cut(s)	50, 158, 309, 843, 1468, 1513, 2047
Bst6I	CTCTTC	3 cut(s)	1254, 1842, 2015
BstAFI	CTTAAG	1 cut(s)	32
BstAPI	GCANNNNNTGC	2 cut(s)	1337, 1883
BstBAI	YACGTR	1 cut(s)	302
BstDEI	CTNAG	7 cut(s)	489, 630, 990, 1164, 1197, 1334, 1841
BstDSI	CCRYGG	1 cut(s)	1173
BstF5I	GGATG	8 cut(s)	420, 480, 977, 1164, 1210, 1377, 1679, 1981
BstFNI	CGCG	2 cut(s)	61, 1922
BstHHI	GCGC	2 cut(s)	426, 1546
BstMAI	GTCTC	1 cut(s)	1444
BstMWI	GCNNNNNNNGC	5 cut(s)	1220, 1337, 1352, 1810, 1883
BstNI	CCWGG	4 cut(s)	250, 1030, 1719, 2028
BstNSI	RCATGY	2 cut(s)	229, 1591
BstSCI	CCNGG	6 cut(s)	248, 770, 1028, 1717, 1820, 2026
BstSFI	CTRYAG	2 cut(s)	49, 2032
BstSLI	GKGCMC	1 cut(s)	1333
BstUI	CGCG	2 cut(s)	61, 1922
BstV1I	GCAGC	4 cut(s)	153, 870, 2018, 2046
BstV2I	GAAGAC	1 cut(s)	588
BstX2I	RGATCY	2 cut(s)	69, 1377
BstXI	CCANNNNNNTGG	1 cut(s)	519
BstYI	RGATCY	2 cut(s)	69, 1377
BsuRI	GGCC	2 cut(s)	285, 1355
BtgI	CCRYGG	1 cut(s)	1173
BtgZI	GCGATG	1 cut(s)	365
BtsCI	GGATG	8 cut(s)	420, 480, 977, 1164, 1210, 1377, 1679, 1981
BtsI	GCAGTG	3 cut(s)	73, 264, 1333
BtsIMutI	CAGTG	7 cut(s)	73, 264, 731, 1333, 1342, 1464, 2052
CaiI	CAGNNNCTG	1 cut(s)	703
CfoI	GCGC	2 cut(s)	426, 1546
Cfr13I	GGNCC	1 cut(s)	1721
CseI	GACGC	4 cut(s)	593, 1277, 1636, 1928
Csp6I	GTAC	1 cut(s)	839
CviAII	CATG	9 cut(s)	226, 448, 1132, 1174, 1382, 1588, 1654, 1741, 1775
CviQI	GTAC	1 cut(s)	839
DdeI	CTNAG	7 cut(s)	489, 630, 990, 1164, 1197, 1334, 1841
DraIII	CACNNNGTG	1 cut(s)	1337
DrdI	GACNNNNNNGTC	1 cut(s)	926
DseDI	GACNNNNNNGTC	1 cut(s)	926
EaeI	YGGCCR	2 cut(s)	283, 1353
Eam1104I	CTCTTC	3 cut(s)	1254, 1842, 2015
EarI	CTCTTC	3 cut(s)	1254, 1842, 2015
Ecl136II	GAGCTC	1 cut(s)	1835
Eco130I	CCWWGG	2 cut(s)	1173, 1753
Eco24I	GRGCYC	2 cut(s)	1815, 1837
Eco31I	GGTCTC	1 cut(s)	1444
Eco32I	GATATC	2 cut(s)	196, 872
Eco47I	GGWCC	1 cut(s)	1721
Eco53kI	GAGCTC	1 cut(s)	1835
Eco57I	CTGAAG	3 cut(s)	600, 1092, 1471
Eco88I	CYCGRG	1 cut(s)	230
EcoICRI	GAGCTC	1 cut(s)	1835
EcoRI	GAATTC	1 cut(s)	1033
EcoRII	CCWGG	4 cut(s)	248, 1028, 1717, 2026
EcoRV	GATATC	2 cut(s)	196, 872
EcoT14I	CCWWGG	2 cut(s)	1173, 1753
EcoT38I	GRGCYC	2 cut(s)	1815, 1837
ErhI	CCWWGG	2 cut(s)	1173, 1753
FaeI	CATG	9 cut(s)	229, 451, 1135, 1177, 1385, 1591, 1657, 1744, 1778
FalI	AAGNNNNNCTT	4 cut(s)	1483, 1515, 1554, 1586
FaqI	GGGAC	2 cut(s)	1671, 2157
FatI	CATG	9 cut(s)	225, 447, 1131, 1173, 1381, 1587, 1653, 1740, 1774
FauI	CCCGC	3 cut(s)	463, 516, 1039
FauNDI	CATATG	1 cut(s)	271
FbaI	TGATCA	3 cut(s)	354, 450, 694
Fnu4HI	GCNGC	4 cut(s)	142, 859, 2032, 2035
FokI	GGATG	8 cut(s)	427, 487, 984, 1171, 1217, 1384, 1686, 1988
FriOI	GRGCYC	2 cut(s)	1815, 1837
Fsp4HI	GCNGC	4 cut(s)	142, 859, 2032, 2035
FspBI	CTAG	4 cut(s)	681, 809, 1661, 1848
FspI	TGCGCA	1 cut(s)	1545
GlaI	GCGC	2 cut(s)	425, 1545
GluI	GCNGC	4 cut(s)	142, 859, 2032, 2035
GsuI	CTGGAG	1 cut(s)	725
HaeIII	GGCC	2 cut(s)	285, 1355
HapII	CCGG	4 cut(s)	419, 771, 1724, 1821
HgaI	GACGC	4 cut(s)	593, 1277, 1636, 1928
HhaI	GCGC	2 cut(s)	426, 1546
Hin1II	CATG	9 cut(s)	229, 451, 1135, 1177, 1385, 1591, 1657, 1744, 1778
Hin6I	GCGC	2 cut(s)	424, 1544
HinP1I	GCGC	2 cut(s)	424, 1544
HindIII	AAGCTT	6 cut(s)	96, 393, 494, 849, 984, 1077
HinfI	GANTC	7 cut(s)	209, 883, 1143, 1792, 1855, 2023, 2096
HpaII	CCGG	4 cut(s)	419, 771, 1724, 1821
HphI	GGTGA	5 cut(s)	163, 203, 401, 1108, 1612
Hpy166II	GTNNAC	6 cut(s)	90, 154, 305, 1125, 1331, 1651
Hpy188I	TCNGA	7 cut(s)	633, 699, 888, 1284, 1490, 1584, 1904
Hpy188III	TCNNGA	6 cut(s)	875, 1140, 1292, 1303, 1868, 2122
Hpy8I	GTNNAC	6 cut(s)	90, 154, 305, 1125, 1331, 1651
Hpy99I	CGWCG	1 cut(s)	1293
HpyAV	CCTTC	9 cut(s)	559, 660, 797, 808, 872, 1195, 1664, 1966, 2044
HpyCH4III	ACNGT	7 cut(s)	50, 158, 309, 843, 1468, 1513, 2047
HpyCH4IV	ACGT	1 cut(s)	301
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1220, 1337, 1352, 1810, 1883
HpyF3I	CTNAG	7 cut(s)	489, 630, 990, 1164, 1197, 1334, 1841
HpySE526I	ACGT	1 cut(s)	301
Hsp92II	CATG	9 cut(s)	229, 451, 1135, 1177, 1385, 1591, 1657, 1744, 1778
HspAI	GCGC	2 cut(s)	424, 1544
Ksp22I	TGATCA	3 cut(s)	354, 450, 694
LguI	GCTCTTC	1 cut(s)	1842
LmnI	GCTCC	4 cut(s)	421, 1584, 1832, 1909
Lsp1109I	GCAGC	4 cut(s)	153, 870, 2018, 2046
MaeI	CTAG	4 cut(s)	681, 809, 1661, 1848
MaeII	ACGT	1 cut(s)	301
MaeIII	GTNAC	1 cut(s)	718
MflI	RGATCY	2 cut(s)	69, 1377
MhlI	GDGCHC	3 cut(s)	1333, 1815, 1837
MlsI	TGGCCA	1 cut(s)	1355
MluNI	TGGCCA	1 cut(s)	1355
MlyI	GAGTC	3 cut(s)	1849, 2032, 2090
MmeI	TCCRAC	1 cut(s)	550
MnlI	CCTC	7 cut(s)	424, 595, 702, 1087, 1255, 1288, 1360
Mox20I	TGGCCA	1 cut(s)	1355
MroXI	GAANNNNTTC	1 cut(s)	882
MscI	TGGCCA	1 cut(s)	1355
MseI	TTAA	7 cut(s)	33, 999, 1040, 1395, 1572, 1686, 2109
MslI	CAYNNNNRTG	2 cut(s)	830, 2132
Msp20I	TGGCCA	1 cut(s)	1355
MspA1I	CMGCKG	1 cut(s)	2037
MspCI	CTTAAG	1 cut(s)	32
MspI	CCGG	4 cut(s)	419, 771, 1724, 1821
MspR9I	CCNGG	6 cut(s)	250, 772, 1030, 1719, 1822, 2028
MvaI	CCWGG	4 cut(s)	250, 1030, 1719, 2028
MvnI	CGCG	2 cut(s)	61, 1922
MwoI	GCNNNNNNNGC	5 cut(s)	1220, 1337, 1352, 1810, 1883
NciI	CCSGG	2 cut(s)	772, 1822
NcoI	CCATGG	1 cut(s)	1173
NdeI	CATATG	1 cut(s)	271
NheI	GCTAGC	1 cut(s)	1847
NlaIII	CATG	9 cut(s)	229, 451, 1135, 1177, 1385, 1591, 1657, 1744, 1778
NlaIV	GGNNCC	2 cut(s)	528, 1911
NsbI	TGCGCA	1 cut(s)	1545
NspI	RCATGY	2 cut(s)	229, 1591
PaeI	GCATGC	2 cut(s)	229, 1591
PciSI	GCTCTTC	1 cut(s)	1842
PcsI	WCGNNNNNNNCGW	1 cut(s)	347
PdmI	GAANNNNTTC	1 cut(s)	882
PfeI	GAWTC	4 cut(s)	209, 883, 1143, 1792
PfoI	TCCNGGA	2 cut(s)	248, 1028
PkrI	GCNGC	4 cut(s)	143, 860, 2033, 2036
PleI	GAGTC	3 cut(s)	1849, 2031, 2090
PpsI	GAGTC	3 cut(s)	1849, 2031, 2090
Ppu21I	YACGTR	1 cut(s)	302
Psp124BI	GAGCTC	1 cut(s)	1837
Psp6I	CCWGG	4 cut(s)	248, 1028, 1717, 2026
PspGI	CCWGG	4 cut(s)	248, 1028, 1717, 2026
PspN4I	GGNNCC	2 cut(s)	528, 1911
PspPI	GGNCC	1 cut(s)	1721
PstI	CTGCAG	1 cut(s)	2036
PstNI	CAGNNNCTG	1 cut(s)	703
PsuI	RGATCY	2 cut(s)	69, 1377
PvuII	CAGCTG	1 cut(s)	2037
RsaI	GTAC	1 cut(s)	840
RsaNI	GTAC	1 cut(s)	839
RseI	CAYNNNNRTG	2 cut(s)	830, 2132
SacI	GAGCTC	1 cut(s)	1837
SapI	GCTCTTC	1 cut(s)	1842
SaqAI	TTAA	7 cut(s)	33, 999, 1040, 1395, 1572, 1686, 2109
SatI	GCNGC	4 cut(s)	142, 859, 2032, 2035
Sau96I	GGNCC	1 cut(s)	1721
SchI	GAGTC	3 cut(s)	1849, 2032, 2090
ScrFI	CCNGG	6 cut(s)	250, 772, 1030, 1719, 1822, 2028
SduI	GDGCHC	3 cut(s)	1333, 1815, 1837
SfcI	CTRYAG	2 cut(s)	49, 2032
SinI	GGWCC	1 cut(s)	1721
SmiMI	CAYNNNNRTG	2 cut(s)	830, 2132
SmlI	CTYRAG	2 cut(s)	32, 93
SmoI	CTYRAG	2 cut(s)	32, 93
SpeI	ACTAGT	1 cut(s)	1660
SphI	GCATGC	2 cut(s)	229, 1591
SsiI	CCGC	3 cut(s)	470, 523, 1046
SspI	AATATT	1 cut(s)	216
SspMI	CTAG	4 cut(s)	681, 809, 1661, 1848
SstI	GAGCTC	1 cut(s)	1837
StyD4I	CCNGG	6 cut(s)	248, 770, 1028, 1717, 1820, 2026
StyI	CCWWGG	2 cut(s)	1173, 1753
TaaI	ACNGT	7 cut(s)	50, 158, 309, 843, 1468, 1513, 2047
TaiI	ACGT	1 cut(s)	304
TaqI	TCGA	8 cut(s)	312, 592, 1291, 1691, 1759, 1785, 1891, 2123
TatI	WGTACW	1 cut(s)	838
TfiI	GAWTC	4 cut(s)	209, 883, 1143, 1792
Tru1I	TTAA	7 cut(s)	33, 999, 1040, 1395, 1572, 1686, 2109
Tru9I	TTAA	7 cut(s)	33, 999, 1040, 1395, 1572, 1686, 2109
TscAI	CASTG	7 cut(s)	80, 271, 738, 1333, 1342, 1471, 2052
TseI	GCWGC	4 cut(s)	141, 858, 2031, 2034
TspGWI	ACGGA	2 cut(s)	195, 1411
TspRI	CASTG	7 cut(s)	80, 271, 738, 1333, 1342, 1471, 2052
Vha464I	CTTAAG	1 cut(s)	32
VneI	GTGCAC	1 cut(s)	1329
VpaK11BI	GGWCC	1 cut(s)	1721
XapI	RAATTY	6 cut(s)	318, 685, 759, 940, 1033, 1429
XceI	RCATGY	2 cut(s)	229, 1591
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2034
XmnI	GAANNNNTTC	1 cut(s)	882
XspI	CTAG	4 cut(s)	681, 809, 1661, 1848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.