MD03G1263000.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010527
Physical Location & Seq
Forward (+)
34893409 .. 34895263
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1263000.v1.1.491

Sequence Viewer

Length: 960 bp
ATGCTTACGATGAGGGACTTCCCTTCTTGTTTTGGGGAAAATGGGGTTCAGATTGCGGATTTTTCGTCATCATCTTCCTCGTCGAGAGCCACAAAAACTGCCCAGAACTTGGTATCCTGCGTCTACCAATGCAAATTAAAGGGCAGATTGTGTTTGATCACTGTGACATGGACCAAGAATCTGATGGGGCAAAGCCTCGCCATCGAAATCGATGATGCAGCCGGTCATTGCCTCTGCAGAGTTGAGATTAAGCCATGGATTTTCTCCAAGAGGAAAGGGTTGAAGAATTTGGAGGTGGACTCTACGAAAATCGACATCTTGTGGGATTTAACAAATGCCAAATTTGGTTCAGGACCTGAGCCATTGGAGAAGTTTTGTCTAGCTCTTATGTTTGATCAAGAAATGGTTCTGTTTCTTGGGGACTTGAAGAGGGAGGATTTCAAAAGCCCTGCTGAGTCCAATTCCAAGTCCAAATCCAAATCCAAATCCAAAGCCGAATTCATAGCGAAAAGAGAGCACATTTTTGGGAAAAAAATTTATGGAGCAAAGGCACAGTTCTGTGATTTGGGCAAAACCCATGACGTGAAAATTGAGTGTGAAACTGCCGGGGGCCTCCATGAGCCATACCTTGTAATCAGCATTGACAGCAAGACTGTGTTGCAGGTCAAGAGGTTGAACTGGAAGTTCAGAGGCAATCACACAATTGTGGTAGATGGGATGAGAGTTGAGGTGTTTTGGGATGTGCACAATTGGCTGTTTGGGAATTCAATGGGAGATGCAGTTTTCATGTTCCAAACTTGCCTCAATGTTGCTGCAGATGAAAGCAAGAAGTTGTGGACTGGTTTGCCAGTTCTGGATCCTTCTGTGTTGAGTTGGTCATCCTCCTCTCAGGAGTTGAGAGATAACCAGTTGCAGGATTTTGGGTTTTCACTGATCTTGCATGCTTGGAAGAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

320

Amino Acids

35.94

Weight (kDa)

8.25

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 34 - 317 3.1e-100 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 652
AccB7I CCANNNNNTGG 1 cut(s) 109
AccI GTMKAC 1 cut(s) 123
AciI CCGC 1 cut(s) 56
AclWI GGATC 2 cut(s) 851, 864
AcsI RAATTY 5 cut(s) 286, 341, 497, 534, 763
AfiI CCNNNNNNNGG 2 cut(s) 109, 913
AgsI TTSAA 5 cut(s) 283, 427, 442, 676, 768
AjiI CACGTC 1 cut(s) 583
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AleI CACNNNNGTG 1 cut(s) 704
AloI GAACNNNNNNTCC 2 cut(s) 98, 130
AluBI AGCT 1 cut(s) 383
AluI AGCT 1 cut(s) 383
Alw21I GWGCWC 2 cut(s) 519, 747
Alw44I GTGCAC 1 cut(s) 743
AlwI GGATC 2 cut(s) 851, 864
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 1 cut(s) 610
ApaLI GTGCAC 1 cut(s) 743
ApeKI GCWGC 2 cut(s) 218, 812
ApoI RAATTY 5 cut(s) 286, 341, 497, 534, 763
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 171, 353, 610
AsuC2I CCSGG 1 cut(s) 607
AvaII GGWCC 2 cut(s) 171, 353
BaeGI GKGCMC 1 cut(s) 747
BamHI GGATCC 1 cut(s) 856
Bbv12I GWGCWC 2 cut(s) 519, 747
BbvI GCAGC 2 cut(s) 230, 799
BccI CCATC 3 cut(s) 178, 209, 707
BciVI GTATCC 1 cut(s) 124
BclI TGATCA 2 cut(s) 156, 394
BcnI CCSGG 1 cut(s) 607
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 2 cut(s) 235, 813
BfuAI ACCTGC 1 cut(s) 652
BfuI GTATCC 1 cut(s) 124
BisI GCNGC 2 cut(s) 219, 813
BlsI GCNGC 2 cut(s) 220, 814
Bme1390I CCNGG 1 cut(s) 607
Bme18I GGWCC 2 cut(s) 171, 353
BmgBI CACGTC 1 cut(s) 583
BmgT120I GGNCC 3 cut(s) 171, 353, 610
BmiI GGNNCC 2 cut(s) 611, 858
BmrFI CCNGG 1 cut(s) 607
BmsI GCATC 2 cut(s) 205, 766
BplI GAGNNNNNCTC 2 cut(s) 284, 316
Bpu10I CCTNAGC 1 cut(s) 357
BpuMI CCSGG 1 cut(s) 607
Bsa29I ATCGAT 1 cut(s) 210
BsaJI CCNNGG 2 cut(s) 254, 606
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 913
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 4 cut(s) 683, 844, 848, 907
Bse3DI GCAATG 1 cut(s) 226
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 254, 606
BseGI GGATG 3 cut(s) 723, 745, 878
BseLI CCNNNNNNNGG 2 cut(s) 109, 913
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 3 cut(s) 348, 444, 902
BseNI ACTGG 4 cut(s) 683, 844, 848, 907
BseRI GAGGAG 1 cut(s) 874
BseSI GKGCMC 1 cut(s) 747
BseXI GCAGC 2 cut(s) 230, 799
BshFI GGCC 1 cut(s) 612
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 519, 747
BsiSI CCGG 2 cut(s) 222, 606
BslFI GGGAC 2 cut(s) 29, 434
BslI CCNNNNNNNGG 2 cut(s) 109, 913
BsmFI GGGAC 2 cut(s) 29, 434
BsnI GGCC 1 cut(s) 612
Bsp1286I GDGCHC 2 cut(s) 519, 747
Bsp143I GATC 4 cut(s) 156, 394, 856, 933
Bsp19I CCATGG 1 cut(s) 254
BspACI CCGC 1 cut(s) 56
BspANI GGCC 1 cut(s) 612
BspCNI CTCAG 3 cut(s) 349, 445, 901
BspDI ATCGAT 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 611, 858
BspMAI CTGCAG 2 cut(s) 239, 817
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 2 cut(s) 851, 864
BsrDI GCAATG 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 4 cut(s) 683, 844, 848, 907
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 254, 606
BssMI GATC 4 cut(s) 156, 394, 856, 933
BssT1I CCWWGG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 163, 555, 655
Bst6I CTCTTC 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 942
BstDEI CTNAG 3 cut(s) 357, 453, 888
BstDSI CCRYGG 1 cut(s) 254
BstF5I GGATG 3 cut(s) 723, 745, 878
BstKTI GATC 4 cut(s) 159, 397, 859, 936
BstMBI GATC 4 cut(s) 156, 394, 856, 933
BstMWI GCNNNNNNNGC 2 cut(s) 645, 751
BstNSI RCATGY 1 cut(s) 944
BstSCI CCNGG 1 cut(s) 605
BstSFI CTRYAG 2 cut(s) 235, 813
BstSLI GKGCMC 1 cut(s) 747
BstV1I GCAGC 2 cut(s) 230, 799
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 1 cut(s) 612
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 254
BtrI CACGTC 1 cut(s) 583
BtsCI GGATG 3 cut(s) 723, 745, 878
BtsIMutI CAGTG 2 cut(s) 159, 929
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 942
CaiI CAGNNNCTG 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 3 cut(s) 171, 353, 610
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 1 cut(s) 109
CviAII CATG 6 cut(s) 168, 255, 578, 617, 787, 941
DdeI CTNAG 3 cut(s) 357, 453, 888
DpnI GATC 4 cut(s) 158, 396, 858, 935
DpnII GATC 4 cut(s) 156, 394, 856, 933
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Eco130I CCWWGG 1 cut(s) 254
Eco47I GGWCC 2 cut(s) 171, 353
EcoO109I RGGNCCY 2 cut(s) 353, 610
EcoRI GAATTC 2 cut(s) 497, 763
EcoT14I CCWWGG 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 6 cut(s) 171, 258, 581, 620, 790, 944
FaqI GGGAC 2 cut(s) 29, 434
FatI CATG 6 cut(s) 167, 254, 577, 616, 786, 940
FbaI TGATCA 2 cut(s) 156, 394
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 2 cut(s) 219, 813
FokI GGATG 3 cut(s) 730, 752, 865
Fsp4HI GCNGC 2 cut(s) 219, 813
FspBI CTAG 1 cut(s) 380
GluI GCNGC 2 cut(s) 219, 813
HaeIII GGCC 1 cut(s) 612
HapII CCGG 2 cut(s) 222, 606
HgaI GACGC 1 cut(s) 109
Hin1II CATG 6 cut(s) 171, 258, 581, 620, 790, 944
HinfI GANTC 3 cut(s) 178, 299, 455
HpaII CCGG 2 cut(s) 222, 606
Hpy166II GTNNAC 4 cut(s) 124, 298, 745, 837
Hpy188I TCNGA 3 cut(s) 51, 183, 689
Hpy188III TCNNGA 6 cut(s) 84, 351, 398, 667, 854, 890
Hpy8I GTNNAC 4 cut(s) 124, 298, 745, 837
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 2 cut(s) 33, 870
HpyCH4III ACNGT 3 cut(s) 163, 555, 655
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 9 cut(s) 132, 218, 237, 661, 745, 779, 815, 913, 940
HpyF10VI GCNNNNNNNGC 2 cut(s) 645, 751
HpyF3I CTNAG 3 cut(s) 357, 453, 888
HpySE526I ACGT 1 cut(s) 582
Hsp92II CATG 6 cut(s) 171, 258, 581, 620, 790, 944
Ksp22I TGATCA 2 cut(s) 156, 394
Kzo9I GATC 4 cut(s) 156, 394, 856, 933
LmnI GCTCC 1 cut(s) 542
Lsp1109I GCAGC 2 cut(s) 230, 799
LweI GCATC 2 cut(s) 205, 766
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 1 cut(s) 163
MalI GATC 4 cut(s) 158, 396, 858, 935
MboI GATC 4 cut(s) 156, 394, 856, 933
MboII GAAGA 3 cut(s) 66, 295, 439
MfeI CAATTG 2 cut(s) 702, 748
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 2 cut(s) 519, 747
MlyI GAGTC 2 cut(s) 293, 464
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 3 cut(s) 137, 249, 329
MslI CAYNNNNRTG 1 cut(s) 704
MspI CCGG 2 cut(s) 222, 606
MspR9I CCNGG 1 cut(s) 607
MunI CAATTG 2 cut(s) 702, 748
MwoI GCNNNNNNNGC 2 cut(s) 645, 751
NciI CCSGG 1 cut(s) 607
NcoI CCATGG 1 cut(s) 254
NdeII GATC 4 cut(s) 156, 394, 856, 933
NlaIII CATG 6 cut(s) 171, 258, 581, 620, 790, 944
NlaIV GGNNCC 2 cut(s) 611, 858
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 1 cut(s) 944
OliI CACNNNNGTG 1 cut(s) 704
PaeI GCATGC 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 109
PkrI GCNGC 2 cut(s) 220, 814
PleI GAGTC 2 cut(s) 293, 463
PpsI GAGTC 2 cut(s) 293, 463
PpuMI RGGWCCY 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 353
PspN4I GGNNCC 2 cut(s) 611, 858
PspPI GGNCC 3 cut(s) 171, 353, 610
PspPPI RGGWCCY 1 cut(s) 353
PstI CTGCAG 2 cut(s) 239, 817
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 856
RseI CAYNNNNRTG 1 cut(s) 704
SaqAI TTAA 3 cut(s) 137, 249, 329
SatI GCNGC 2 cut(s) 219, 813
Sau3AI GATC 4 cut(s) 156, 394, 856, 933
Sau96I GGNCC 3 cut(s) 171, 353, 610
SchI GAGTC 2 cut(s) 293, 464
ScrFI CCNGG 1 cut(s) 607
SduI GDGCHC 2 cut(s) 519, 747
SetI ASST 8 cut(s) 297, 358, 385, 585, 630, 666, 674, 732
SfaNI GCATC 2 cut(s) 205, 766
SfcI CTRYAG 2 cut(s) 235, 813
SinI GGWCC 2 cut(s) 171, 353
SmiMI CAYNNNNRTG 1 cut(s) 704
SphI GCATGC 1 cut(s) 944
SsiI CCGC 1 cut(s) 56
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 605
StyI CCWWGG 1 cut(s) 254
TaaI ACNGT 3 cut(s) 163, 555, 655
TaiI ACGT 1 cut(s) 585
TaqI TCGA 4 cut(s) 83, 204, 210, 312
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 3 cut(s) 137, 249, 329
Tru9I TTAA 3 cut(s) 137, 249, 329
TscAI CASTG 2 cut(s) 166, 936
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 2 cut(s) 218, 812
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 3 cut(s) 490, 775, 834
TspRI CASTG 2 cut(s) 166, 936
Van91I CCANNNNNTGG 1 cut(s) 109
VneI GTGCAC 1 cut(s) 743
VpaK11BI GGWCC 2 cut(s) 171, 353
XapI RAATTY 5 cut(s) 286, 341, 497, 534, 763
XceI RCATGY 1 cut(s) 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.