Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011280

Physical Location & Seq

Genomic Coordinates Forward (+)

36340647 .. 36344425

Gene Structure 3779 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1283400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1428 bp

ATGAAGGGAGCGCTGGCGAGGCTTCAGCTAGGGGAAACCCTGAACCAATCGTCGTTTTCCACGTCATCATCCAACCCCTCTCGCTTCCAGAACCTTGTAGAAGCCCGAGCCGCCGACGGCAGCCTTCCCTCCTCCGCTCCCCTCGGCACCGACGTCGACACCGATGTCGACGACAGGGAGTTCATTCTCAGTCAAGATTTCTTCTGCACTCCTGATTATATCACACCGGATAATCAACATATGATGAACGGCTGGGATTGCACCAAGCTGGAAAACATCCCTTGCCCCAAATCACCCGAGAAATCGAATACCCTTAAATCGAAGAAGCGAAGACAGTGTCAGAATGATATCTCATTGAATCCTCTCAGCCCTACATTGTCTGGAAATCAGCCGATAGTTGAGCTTGAAGCAGATGTTTTTGGCATGGATGAGGTCATGATGGAGAAACCAACAGCAACAGAACCACGGAAGGTGCAAAATTATGTATCTCAATCTGCTGTGGCTTTACGCTGTCGTGTCATGCCTCCTCCTTGCTTGAAGAATCCGTACATTAAGGATGCTTCAGAAATGGGCATTGATCCTCTTGGCAGCCAAAGATCGAAATGTGCAGCTTTCTTCCCTACTTTTATGGGCGGAAATGGCCTTTCACGTTACTGTGCTGATTTTCACGAGATTGAGCAAATCGGCAGTGGGTACTTCAGTCACGTATTCAAAGTCTTGAACAGAATTGATGGTTGTTTGTATGCCGTGAAACGTAGCATGAAGCAGTTGCATCTGGACACAGAAAGGAGAAAGGCTATGATGGAAGTTCAATCTCTGGCAGCTTTAGGGTCCCATGAAAACATTGTTGGATACTACTCGTCTTGGTTTGAAAATGAGCAGCTCCACATTCAGATGGAATTATGTGATCACAGCTTATCCATAAACAAGTTGTCCCGACCATTCAAAGATGGGGAGATTTTAGAAGCCTTGTATCAGATTGCAAAAGCGTTGCAGTTTATGCATGGGAGAGGAATAGTTCACTTAGATGTAAAACCTGATAATATATATGTGAAGAATGGTATTTATAAGCTTGGTGACTTTGGATGTGCAACTCTACTTGATAAGAGCCTACCGATTGAAGAGGGTGATGCTCGATATACGCCTCAAGAGATCCTGAATGAGAAATATGATGATCTTGACAAGGCTGATATCTTCTCATTGGGAGTCACTATTTACGAGCTGATTAGAGGTTCTCCTTTACCAGAATCAGGGCCTCAAATTTTAAATCTTAGGGAGGGAAAGTTGCCACTTCTTCCTGGTCATTCATTGCAATTTCAGAACTTACTCAAGGTCATGTTGGATCCAAATCCAGTCTGGAGACCGTCAGCGAAAGATTTGGTTGAAAATCCAAATTTTTACAAGGTGCGGCGAAATGGACAAGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000076 GO:0003674 GO:0003824 GO:0004672 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007050 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0031570 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04110 map04110

Pfam Domains

Protein Families

Pkinase

Protein Analysis

476

Amino Acids

53.28

Weight (kDa)

5.77

Isoelectric Point (pI)

45.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	222 - 464	6e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	225 - 462	1.5e-22	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011280)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02970
fragaria_vesca	FvH4_c6g00040 FvH4_c6g00040 FvH4_c6g00040 FvH4_c6g00040
malus_domestica	MD03G1283400.v1.1 MD11G1303200.v1.1
prunus_persica	Prupe.8G256300_v2.0.a1
pyrus_communis	pycom03g22420 pycom11g26490
rosa_chinensis	RchiOBHm_Chr6g0293261
rosa_laevigata	RLG00000011963
rosa_multiflora	Rmu_ssc0000162.1_g000031
rosa_roxburghii	Rroxscaffold_7G00174300
rosa_rugosa	Rorug06G0233800
rosa_samantha	Rh6AG346800 Rh6BG354200 Rh6CG360700 Rh6DG347200
rosa_wichuraiana	Rw6G030190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1068
AasI	GACNNNNNNGTC	1 cut(s)	164
AatII	GACGTC	1 cut(s)	156
AccB1I	GGYRCC	1 cut(s)	146
AccBSI	CCGCTC	1 cut(s)	137
AccI	GTMKAC	2 cut(s)	156, 168
AciI	CCGC	4 cut(s)	111, 135, 633, 1408
AclWI	GGATC	4 cut(s)	572, 1147, 1337, 1350
AcsI	RAATTY	2 cut(s)	1260, 1393
AcuI	CTGAAG	3 cut(s)	8, 546, 682
AcyI	GRCGYC	1 cut(s)	153
AfaI	GTAC	2 cut(s)	548, 695
AfeI	AGCGCT	1 cut(s)	12
AfiI	CCNNNNNNNGG	1 cut(s)	1250
AjiI	CACGTC	1 cut(s)	63
AjnI	CCWGG	1 cut(s)	1297
AjuI	GAANNNNNNNTTGG	2 cut(s)	831, 863
AluBI	AGCT	9 cut(s)	28, 268, 403, 611, 824, 883, 915, 1072, 1222
AluI	AGCT	9 cut(s)	28, 268, 403, 611, 824, 883, 915, 1072, 1222
Alw26I	GTCTC	1 cut(s)	1354
AlwI	GGATC	4 cut(s)	572, 1147, 1337, 1350
Ama87I	CYCGRG	2 cut(s)	105, 296
Aor51HI	AGCGCT	1 cut(s)	12
AoxI	GGCC	2 cut(s)	640, 1253
ApeKI	GCWGC	5 cut(s)	120, 588, 608, 821, 880
ApoI	RAATTY	2 cut(s)	1260, 1393
ArsI	GACNNNNNNTTYG	2 cut(s)	322, 354
AspLEI	GCGC	1 cut(s)	13
AspS9I	GGNCC	2 cut(s)	831, 1253
AsuHPI	GGTGA	3 cut(s)	285, 1088, 1139
AvaI	CYCGRG	2 cut(s)	105, 296
AvaII	GGWCC	1 cut(s)	831
BaeI	ACNNNNGTAYC	2 cut(s)	685, 718
BamHI	GGATCC	1 cut(s)	1342
BanI	GGYRCC	1 cut(s)	146
BarI	GAAGNNNNNNTAC	2 cut(s)	530, 562
BauI	CACGAG	1 cut(s)	668
BbsI	GAAGAC	1 cut(s)	337
BbvI	GCAGC	5 cut(s)	132, 600, 620, 833, 892
BccI	CCATC	5 cut(s)	433, 725, 796, 889, 944
BceAI	ACGGC	3 cut(s)	133, 265, 731
BcgI	CGANNNNNNTGC	2 cut(s)	136, 170
BciT130I	CCWGG	1 cut(s)	1299
BciVI	GTATCC	1 cut(s)	845
BclI	TGATCA	1 cut(s)	907
BcoDI	GTCTC	1 cut(s)	1354
BfaI	CTAG	2 cut(s)	29, 1426
BfoI	RGCGCY	1 cut(s)	14
BfuI	GTATCC	1 cut(s)	845
BisI	GCNGC	7 cut(s)	111, 121, 589, 609, 822, 881, 1409
BlsI	GCNGC	7 cut(s)	112, 122, 590, 610, 823, 882, 1410
Bme1390I	CCNGG	1 cut(s)	1299
Bme18I	GGWCC	1 cut(s)	831
BmeT110I	CYCGRG	2 cut(s)	105, 296
BmgBI	CACGTC	1 cut(s)	63
BmgT120I	GGNCC	2 cut(s)	831, 1253
BmiI	GGNNCC	4 cut(s)	148, 832, 833, 1344
BmrFI	CCNGG	1 cut(s)	1299
BmsI	GCATC	3 cut(s)	547, 781, 1120
BpiI	GAAGAC	1 cut(s)	337
BpmI	CTGGAG	1 cut(s)	1378
BpuEI	CTTGAG	2 cut(s)	1131, 1313
BsaAI	YACGTR	1 cut(s)	706
BsaBI	GATNNNNATC	1 cut(s)	1347
BsaHI	GRCGYC	1 cut(s)	153
BsaI	GGTCTC	1 cut(s)	1354
BsaJI	CCNNGG	2 cut(s)	142, 464
BsaWI	WCCGGW	1 cut(s)	226
BsaXI	ACNNNNNCTCC	2 cut(s)	1268, 1298
Bsc4I	CCNNNNNNNGG	1 cut(s)	1250
Bse1I	ACTGG	1 cut(s)	1352
Bse3DI	GCAATG	1 cut(s)	1307
Bse8I	GATNNNNATC	1 cut(s)	1347
BseBI	CCWGG	1 cut(s)	1299
BseDI	CCNNGG	2 cut(s)	142, 464
BseGI	GGATG	5 cut(s)	68, 276, 433, 562, 1091
BseJI	GATNNNNATC	1 cut(s)	1347
BseLI	CCNNNNNNNGG	1 cut(s)	1250
BseMI	GCAATG	1 cut(s)	1307
BseMII	CTCAG	2 cut(s)	202, 379
BseNI	ACTGG	1 cut(s)	1352
BseRI	GAGGAG	2 cut(s)	121, 516
BseXI	GCAGC	5 cut(s)	132, 600, 620, 833, 892
BseYI	CCCAGC	1 cut(s)	252
BsgI	GTGCAG	2 cut(s)	190, 627
BshFI	GGCC	2 cut(s)	642, 1255
BshNI	GGYRCC	1 cut(s)	146
BsiHKCI	CYCGRG	2 cut(s)	105, 296
BsiSI	CCGG	1 cut(s)	227
BslFI	GGGAC	2 cut(s)	817, 919
BslI	CCNNNNNNNGG	1 cut(s)	1250
BsmAI	GTCTC	1 cut(s)	1354
BsmFI	GGGAC	2 cut(s)	817, 919
BsnI	GGCC	2 cut(s)	642, 1255
Bso31I	GGTCTC	1 cut(s)	1354
BsoBI	CYCGRG	2 cut(s)	105, 296
Bsp143I	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
BspACI	CCGC	4 cut(s)	111, 135, 633, 1408
BspANI	GGCC	2 cut(s)	642, 1255
BspCNI	CTCAG	2 cut(s)	201, 378
BspHI	TCATGA	1 cut(s)	435
BspLI	GGNNCC	4 cut(s)	148, 832, 833, 1344
BspPI	GGATC	4 cut(s)	572, 1147, 1337, 1350
BspT107I	GGYRCC	1 cut(s)	146
BspTNI	GGTCTC	1 cut(s)	1354
BsrBI	CCGCTC	1 cut(s)	137
BsrDI	GCAATG	1 cut(s)	1307
BsrI	ACTGG	1 cut(s)	1352
BssECI	CCNNGG	2 cut(s)	142, 464
BssMI	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
BssNI	GRCGYC	1 cut(s)	153
BssSI	CACGAG	1 cut(s)	668
Bst2BI	CACGAG	1 cut(s)	668
Bst2UI	CCWGG	1 cut(s)	1299
Bst4CI	ACNGT	3 cut(s)	336, 656, 1365
Bst6I	CTCTTC	1 cut(s)	1116
BstACI	GRCGYC	1 cut(s)	153
BstAPI	GCANNNNNTGC	1 cut(s)	1000
BstBAI	YACGTR	1 cut(s)	706
BstC8I	GCNNGC	1 cut(s)	15
BstDEI	CTNAG	4 cut(s)	188, 365, 1024, 1271
BstDSI	CCRYGG	1 cut(s)	464
BstF5I	GGATG	5 cut(s)	68, 276, 433, 562, 1091
BstH2I	RGCGCY	1 cut(s)	14
BstHHI	GCGC	1 cut(s)	13
BstKTI	GATC	6 cut(s)	580, 599, 910, 1155, 1177, 1345
BstMAI	GTCTC	1 cut(s)	1354
BstMBI	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
BstMWI	GCNNNNNNNGC	5 cut(s)	19, 110, 258, 639, 1000
BstNI	CCWGG	1 cut(s)	1299
BstSCI	CCNGG	1 cut(s)	1297
BstV1I	GCAGC	5 cut(s)	132, 600, 620, 833, 892
BstV2I	GAAGAC	1 cut(s)	337
BstX2I	RGATCY	2 cut(s)	1152, 1342
BstYI	RGATCY	2 cut(s)	1152, 1342
BsuI	GTATCC	1 cut(s)	845
BsuRI	GGCC	2 cut(s)	642, 1255
BtgI	CCRYGG	1 cut(s)	464
BtrI	CACGTC	1 cut(s)	63
BtsCI	GGATG	5 cut(s)	68, 276, 433, 562, 1091
BtsI	GCAGTG	1 cut(s)	694
BtsIMutI	CAGTG	2 cut(s)	341, 694
Cac8I	GCNNGC	1 cut(s)	15
CciI	TCATGA	1 cut(s)	435
CfoI	GCGC	1 cut(s)	13
Cfr13I	GGNCC	2 cut(s)	831, 1253
Csp6I	GTAC	2 cut(s)	547, 694
CviAII	CATG	7 cut(s)	424, 436, 520, 760, 836, 1004, 1336
CviQI	GTAC	2 cut(s)	547, 694
DdeI	CTNAG	4 cut(s)	188, 365, 1024, 1271
DpnI	GATC	6 cut(s)	579, 598, 909, 1154, 1176, 1344
DpnII	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
DraI	TTTAAA	1 cut(s)	1266
DrdI	GACNNNNNNGTC	1 cut(s)	164
DseDI	GACNNNNNNGTC	1 cut(s)	164
Eam1104I	CTCTTC	1 cut(s)	1116
EarI	CTCTTC	1 cut(s)	1116
EciI	GGCGGA	1 cut(s)	648
Eco31I	GGTCTC	1 cut(s)	1354
Eco32I	GATATC	2 cut(s)	349, 1192
Eco47I	GGWCC	1 cut(s)	831
Eco47III	AGCGCT	1 cut(s)	12
Eco57I	CTGAAG	3 cut(s)	8, 546, 682
Eco88I	CYCGRG	2 cut(s)	105, 296
EcoO109I	RGGNCCY	2 cut(s)	831, 1253
EcoRII	CCWGG	1 cut(s)	1297
EcoRV	GATATC	2 cut(s)	349, 1192
EcoT22I	ATGCAT	1 cut(s)	1005
FaeI	CATG	7 cut(s)	427, 439, 523, 763, 839, 1007, 1339
FaqI	GGGAC	2 cut(s)	817, 919
FatI	CATG	7 cut(s)	423, 435, 519, 759, 835, 1003, 1335
FauNDI	CATATG	1 cut(s)	240
FbaI	TGATCA	1 cut(s)	907
FblI	GTMKAC	2 cut(s)	156, 168
Fnu4HI	GCNGC	7 cut(s)	111, 121, 589, 609, 822, 881, 1409
FokI	GGATG	5 cut(s)	55, 263, 440, 569, 1098
Fsp4HI	GCNGC	7 cut(s)	111, 121, 589, 609, 822, 881, 1409
FspBI	CTAG	2 cut(s)	29, 1426
GlaI	GCGC	1 cut(s)	12
GluI	GCNGC	7 cut(s)	111, 121, 589, 609, 822, 881, 1409
GsaI	CCCAGC	1 cut(s)	256
GsuI	CTGGAG	1 cut(s)	1378
HaeII	RGCGCY	1 cut(s)	14
HaeIII	GGCC	2 cut(s)	642, 1255
HapII	CCGG	1 cut(s)	227
HhaI	GCGC	1 cut(s)	13
Hin1I	GRCGYC	1 cut(s)	153
Hin1II	CATG	7 cut(s)	427, 439, 523, 763, 839, 1007, 1339
Hin6I	GCGC	1 cut(s)	11
HinP1I	GCGC	1 cut(s)	11
HincII	GTYRAC	2 cut(s)	157, 169
HindII	GTYRAC	2 cut(s)	157, 169
HindIII	AAGCTT	1 cut(s)	1070
HinfI	GANTC	4 cut(s)	358, 541, 1206, 1247
HpaII	CCGG	1 cut(s)	227
HphI	GGTGA	3 cut(s)	285, 1088, 1139
Hpy166II	GTNNAC	3 cut(s)	157, 169, 1021
Hpy188I	TCNGA	5 cut(s)	342, 565, 894, 978, 1320
Hpy8I	GTNNAC	3 cut(s)	157, 169, 1021
Hpy99I	CGWCG	5 cut(s)	55, 119, 155, 158, 173
HpyAV	CCTTC	2 cut(s)	134, 463
HpyCH4III	ACNGT	3 cut(s)	336, 656, 1365
HpyCH4IV	ACGT	5 cut(s)	62, 153, 649, 705, 754
HpyF10VI	GCNNNNNNNGC	5 cut(s)	19, 110, 258, 639, 1000
HpyF3I	CTNAG	4 cut(s)	188, 365, 1024, 1271
HpySE526I	ACGT	5 cut(s)	62, 153, 649, 705, 754
Hsp92I	GRCGYC	1 cut(s)	153
Hsp92II	CATG	7 cut(s)	427, 439, 523, 763, 839, 1007, 1339
HspAI	GCGC	1 cut(s)	11
KflI	GGGWCCC	1 cut(s)	831
Ksp22I	TGATCA	1 cut(s)	907
Kzo9I	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
LmnI	GCTCC	3 cut(s)	8, 142, 888
Lsp1109I	GCAGC	5 cut(s)	132, 600, 620, 833, 892
LweI	GCATC	3 cut(s)	547, 781, 1120
MaeI	CTAG	2 cut(s)	29, 1426
MaeII	ACGT	5 cut(s)	62, 153, 649, 705, 754
MaeIII	GTNAC	4 cut(s)	650, 701, 1076, 1207
MalI	GATC	6 cut(s)	579, 598, 909, 1154, 1176, 1344
MbiI	CCGCTC	1 cut(s)	137
MboI	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
MboII	GAAGA	9 cut(s)	193, 334, 342, 550, 607, 1066, 1133, 1186, 1286
MflI	RGATCY	2 cut(s)	1152, 1342
MluCI	AATT	6 cut(s)	478, 726, 899, 1260, 1313, 1393
MlyI	GAGTC	1 cut(s)	1215
MmeI	TCCRAC	3 cut(s)	96, 829, 1320
Mph1103I	ATGCAT	1 cut(s)	1005
MseI	TTAA	3 cut(s)	315, 552, 1265
MslI	CAYNNNNRTG	2 cut(s)	893, 1026
MspI	CCGG	1 cut(s)	227
MspR9I	CCNGG	1 cut(s)	1299
MvaI	CCWGG	1 cut(s)	1299
MwoI	GCNNNNNNNGC	5 cut(s)	19, 110, 258, 639, 1000
NdeI	CATATG	1 cut(s)	240
NdeII	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
NlaIII	CATG	7 cut(s)	427, 439, 523, 763, 839, 1007, 1339
NlaIV	GGNNCC	4 cut(s)	148, 832, 833, 1344
NmeAIII	GCCGAG	1 cut(s)	123
NmuCI	GTSAC	3 cut(s)	701, 1076, 1207
NsiI	ATGCAT	1 cut(s)	1005
PagI	TCATGA	1 cut(s)	435
PcsI	WCGNNNNNNNCGW	3 cut(s)	59, 150, 159
PfeI	GAWTC	3 cut(s)	358, 541, 1247
PflFI	GACNNNGTC	1 cut(s)	336
PkrI	GCNGC	7 cut(s)	112, 122, 590, 610, 823, 882, 1410
PleI	GAGTC	1 cut(s)	1214
PpsI	GAGTC	1 cut(s)	1214
Ppu21I	YACGTR	1 cut(s)	706
PpuMI	RGGWCCY	1 cut(s)	831
PsiI	TTATAA	1 cut(s)	1068
Psp5II	RGGWCCY	1 cut(s)	831
Psp6I	CCWGG	1 cut(s)	1297
PspFI	CCCAGC	1 cut(s)	252
PspGI	CCWGG	1 cut(s)	1297
PspN4I	GGNNCC	4 cut(s)	148, 832, 833, 1344
PspPI	GGNCC	2 cut(s)	831, 1253
PspPPI	RGGWCCY	1 cut(s)	831
PsuI	RGATCY	2 cut(s)	1152, 1342
PsyI	GACNNNGTC	1 cut(s)	336
RsaI	GTAC	2 cut(s)	548, 695
RsaNI	GTAC	2 cut(s)	547, 694
RseI	CAYNNNNRTG	2 cut(s)	893, 1026
SalI	GTCGAC	2 cut(s)	155, 167
SaqAI	TTAA	3 cut(s)	315, 552, 1265
SatI	GCNGC	7 cut(s)	111, 121, 589, 609, 822, 881, 1409
Sau3AI	GATC	6 cut(s)	577, 596, 907, 1152, 1174, 1342
Sau96I	GGNCC	2 cut(s)	831, 1253
SchI	GAGTC	1 cut(s)	1215
ScrFI	CCNGG	1 cut(s)	1299
SfaNI	GCATC	3 cut(s)	547, 781, 1120
SinI	GGWCC	1 cut(s)	831
SmiMI	CAYNNNNRTG	2 cut(s)	893, 1026
SmlI	CTYRAG	2 cut(s)	1146, 1328
SmoI	CTYRAG	2 cut(s)	1146, 1328
Sse9I	AATT	6 cut(s)	478, 726, 899, 1260, 1313, 1393
SsiI	CCGC	4 cut(s)	111, 135, 633, 1408
SspMI	CTAG	2 cut(s)	29, 1426
StyD4I	CCNGG	1 cut(s)	1297
TaaI	ACNGT	3 cut(s)	336, 656, 1365
TaiI	ACGT	5 cut(s)	65, 156, 652, 708, 757
TaqI	TCGA	6 cut(s)	156, 168, 305, 320, 599, 1135
TasI	AATT	6 cut(s)	478, 726, 899, 1260, 1313, 1393
TauI	GCSGC	2 cut(s)	113, 1411
TfiI	GAWTC	3 cut(s)	358, 541, 1247
Tru1I	TTAA	3 cut(s)	315, 552, 1265
Tru9I	TTAA	3 cut(s)	315, 552, 1265
TscAI	CASTG	2 cut(s)	341, 694
TseFI	GTSAC	3 cut(s)	701, 1076, 1207
TseI	GCWGC	5 cut(s)	120, 588, 608, 821, 880
Tsp45I	GTSAC	3 cut(s)	701, 1076, 1207
TspDTI	ATGAA	6 cut(s)	17, 172, 260, 776, 852, 1296
TspGWI	ACGGA	2 cut(s)	481, 534
TspRI	CASTG	2 cut(s)	341, 694
Tth111I	GACNNNGTC	1 cut(s)	336
VpaK11BI	GGWCC	1 cut(s)	831
XapI	RAATTY	2 cut(s)	1260, 1393
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1353
XmiI	GTMKAC	2 cut(s)	156, 168
XspI	CTAG	2 cut(s)	29, 1426
ZraI	GACGTC	1 cut(s)	154
Zsp2I	ATGCAT	1 cut(s)	1005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.