RchiOBHm_Chr6g0293261
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011280
Physical Location & Seq
Reverse (-)
55997264 .. 56001242
3979 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26314

Sequence Viewer

Length: 1419 bp
ATGAGCGCCATGAAGGGACCACTAGAAAGGCTTCACCTAAGTGAAAGCCACCACCAATCACCGTTCCCCAATTCCGACCCAGCTCGATTCCAGTGCCTTCTGGACTCCGTCGCCTCCGACCCCAGTCTTCCTTCTTCCTCGTCTCTCGGTGCTGACCTCGATCCCAATTTGGATGACAAGGATGGCATTCTCAGCCAGGACTTCTTCTGCACTCCAGATTATATCACCCCGGAAAATCAGAATACGTTTAACGGGTTTGATTGCAGTAAGGAAAACATCCCTTGCCCCAAATCTCCGGAGAAAACTACGGTTAAATCCAAGAGGCGTCGACAGGGGCATTATGTTATCTCATCGGATCCTGTTCTTAGCCCCACTTTGTTTGGGAATCAGGAGGTGGGGAAAGATGCAATTGGAATAGATGATATTGAGATAGAGAACACAACTGTAAGTGAAGTGCGGAAGACAACAAATTATGTATCCCAATCTGCAGTTGCTCTGCGCTGTCGGGTTACGCCTCCTCCATGCTTTAAGAATCCTTATTTAAAGGATGCTTCACAAATGGAGATTGATCCTTTTGGCAATCAAAGGTCGAAATGTGCAGCTTTCTTACCTGCAATGAAGGATGGAAATGGCCTTTCACGTTATCGTGCTGATTTTCATGAGATTGAGCAAATTGGGAGGGGGAACTTCAGTCATGTATTTAAAGTCTTGAACCGAATTGATGGTTGCTTGTATGCTGTGAAACAAAGCATTAGGCAGTTGCATCAGGACACAGAAAGGAGAAAAGCTTTAATGGAAGTTCAATGCTTAGCAGCTCTAGGATCCCATGCAAATATTGTTGGGTACTACACTTCTTGGTTTGAAAACGAGAAGCTCTACATTCAGATGGAGCTCTGTGATCACAGCTTATGTGTAGACAGTTTTTCCAGACGTTTCACAGAGGGAGAGATTTTAGTAGCCTTGTATCAGATTGCAAAGGCATTGCAGTTTATGCATGAGAGAGGAATAGCTCACTTAGATGTAAAGCCAGATAATATATATGTGAAGAATGGTATTTATAAGCTTGGTGACTTTGGATGTGCAACACTACTTGATAAAAGCCTACCAATTGAAGAGGGTGATGCTCGCTATATGCCTTCAGAGATCCTGAATGAGAAACATGACGATCTTGACAAGGCTGATATCTTCTCATTGGGAGTTGCTATGTATGAGCTTATTAGAGGTTCACCGTTACCAGAAGAAGGACCTCAAACCTTAAATCTTAAGAAAGGAAAATTGCCACTGCTTCCTGGTCACTCCTTGCAACTTCAGAACTTGCTCAAGGCCATGTTGGATCCAAATCCAGTCTGCAGACCATCAGCTAAAGAGTTGGTTGAAAATCTAATGTTCCACAGGGTGCTAAAAAATGCATGGGCCTAG

Protein Analysis

472

Amino Acids

52.91

Weight (kDa)

5.9

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 219 - 460 2.9e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 222 - 461 1.7e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02970
fragaria_vesca FvH4_c6g00040 FvH4_c6g00040 FvH4_c6g00040 FvH4_c6g00040
malus_domestica MD03G1283400.v1.1 MD11G1303200.v1.1
prunus_persica Prupe.8G256300_v2.0.a1
pyrus_communis pycom03g22420 pycom11g26490
rosa_chinensis RchiOBHm_Chr6g0293261
rosa_laevigata RLG00000011963
rosa_multiflora Rmu_ssc0000162.1_g000031
rosa_roxburghii Rroxscaffold_7G00174300
rosa_rugosa Rorug06G0233800
rosa_samantha Rh6AG346800 Rh6BG354200 Rh6CG360700 Rh6DG347200
rosa_wichuraiana Rw6G030190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 619
AccI GTMKAC 2 cut(s) 328, 915
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 1 cut(s) 457
AclWI GGATC 9 cut(s) 155, 350, 363, 563, 816, 829, 1138, 1328, 1341
AcuI CTGAAG 3 cut(s) 673, 1122, 1292
AcyI GRCGYC 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 1396
AfaI GTAC 1 cut(s) 845
AfiI CCNNNNNNNGG 1 cut(s) 1241
AflII CTTAAG 1 cut(s) 1262
AgsI TTSAA 5 cut(s) 712, 803, 863, 1112, 1376
AjnI CCWGG 2 cut(s) 195, 1288
AleI CACNNNNGTG 1 cut(s) 39
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 9 cut(s) 155, 350, 363, 563, 816, 829, 1138, 1328, 1341
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 3 cut(s) 631, 1323, 1413
ApeKI GCWGC 2 cut(s) 599, 812
Asp700I GAANNNNTTC 1 cut(s) 30
AspLEI GCGC 2 cut(s) 8, 501
AspS9I GGNCC 3 cut(s) 17, 1244, 1413
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 6 cut(s) 26, 51, 217, 1079, 1130, 1218
AvaII GGWCC 2 cut(s) 17, 1244
BamHI GGATCC 3 cut(s) 355, 821, 1333
BanII GRGCYC 1 cut(s) 894
BbsI GAAGAC 2 cut(s) 119, 467
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 2 cut(s) 611, 824
BccI CCATC 5 cut(s) 176, 617, 716, 880, 1363
BcgI CGANNNNNNTGC 2 cut(s) 75, 109
BciT130I CCWGG 2 cut(s) 197, 1290
BciVI GTATCC 1 cut(s) 487
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 3 cut(s) 23, 818, 1417
BfmI CTRYAG 2 cut(s) 486, 1348
BfoI RGCGCY 1 cut(s) 9
BfrI CTTAAG 1 cut(s) 1262
BfuAI ACCTGC 1 cut(s) 619
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 2 cut(s) 600, 813
BlpI GCTNAGC 1 cut(s) 808
BlsI GCNGC 2 cut(s) 601, 814
Bme1390I CCNGG 3 cut(s) 197, 230, 1290
Bme18I GGWCC 2 cut(s) 17, 1244
BmgT120I GGNCC 3 cut(s) 17, 1244, 1413
BmiI GGNNCC 4 cut(s) 18, 357, 823, 1335
BmrFI CCNGG 3 cut(s) 197, 230, 1290
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 4 cut(s) 394, 538, 772, 1111
BmuI ACTGGG 1 cut(s) 117
BoxI GACNNNNGTC 1 cut(s) 123
BpiI GAAGAC 2 cut(s) 119, 467
BpmI CTGGAG 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 808
BpuEI CTTGAG 1 cut(s) 1304
BpuMI CCSGG 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 1338
BsaHI GRCGYC 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 1241
Bse1I ACTGG 3 cut(s) 91, 123, 1343
Bse3DI GCAATG 2 cut(s) 621, 980
Bse8I GATNNNNATC 1 cut(s) 1338
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 2 cut(s) 197, 1290
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 6 cut(s) 178, 187, 276, 553, 628, 1082
BseJI GATNNNNATC 1 cut(s) 1338
BseLI CCNNNNNNNGG 1 cut(s) 1241
BseMI GCAATG 2 cut(s) 621, 980
BseMII CTCAG 1 cut(s) 205
BseNI ACTGG 3 cut(s) 91, 123, 1343
BseRI GAGGAG 1 cut(s) 507
BseXI GCAGC 2 cut(s) 611, 824
BseYI CCCAGC 1 cut(s) 79
BsgI GTGCAG 2 cut(s) 193, 618
BshFI GGCC 3 cut(s) 633, 1325, 1415
BsiHKAI GWGCWC 1 cut(s) 894
BsiSI CCGG 2 cut(s) 230, 296
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 1241
BsmAI GTCTC 1 cut(s) 147
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 3 cut(s) 633, 1325, 1415
Bsp1286I GDGCHC 1 cut(s) 894
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
Bsp1720I GCTNAGC 1 cut(s) 808
BspACI CCGC 1 cut(s) 457
BspANI GGCC 3 cut(s) 633, 1325, 1415
BspCNI CTCAG 1 cut(s) 204
BspEI TCCGGA 1 cut(s) 295
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 4 cut(s) 18, 357, 823, 1335
BspMAI CTGCAG 2 cut(s) 490, 1352
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 9 cut(s) 155, 350, 363, 563, 816, 829, 1138, 1328, 1341
BspTI CTTAAG 1 cut(s) 1262
BsrDI GCAATG 2 cut(s) 621, 980
BsrI ACTGG 3 cut(s) 91, 123, 1343
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
BssNI GRCGYC 1 cut(s) 325
Bst2UI CCWGG 2 cut(s) 197, 1290
Bst4CI ACNGT 5 cut(s) 63, 310, 445, 920, 1230
Bst6I CTCTTC 1 cut(s) 1107
BstACI GRCGYC 1 cut(s) 325
BstAFI CTTAAG 1 cut(s) 1262
BstAPI GCANNNNNTGC 1 cut(s) 991
BstC8I GCNNGC 1 cut(s) 1126
BstDEI CTNAG 5 cut(s) 38, 191, 365, 808, 1015
BstF5I GGATG 6 cut(s) 178, 187, 276, 553, 628, 1082
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 2 cut(s) 8, 501
BstKTI GATC 8 cut(s) 163, 358, 571, 824, 901, 1146, 1168, 1336
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
BstMWI GCNNNNNNNGC 2 cut(s) 192, 991
BstNI CCWGG 2 cut(s) 197, 1290
BstPAI GACNNNNGTC 1 cut(s) 123
BstSCI CCNGG 3 cut(s) 195, 228, 1288
BstSFI CTRYAG 2 cut(s) 486, 1348
BstV1I GCAGC 2 cut(s) 611, 824
BstV2I GAAGAC 2 cut(s) 119, 467
BstX2I RGATCY 4 cut(s) 355, 821, 1143, 1333
BstYI RGATCY 4 cut(s) 355, 821, 1143, 1333
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 3 cut(s) 633, 1325, 1415
BtsCI GGATG 6 cut(s) 178, 187, 276, 553, 628, 1082
BtsI GCAGTG 1 cut(s) 1280
BtsIMutI CAGTG 2 cut(s) 98, 1280
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 1 cut(s) 1126
CciI TCATGA 1 cut(s) 658
CfoI GCGC 2 cut(s) 8, 501
Cfr13I GGNCC 3 cut(s) 17, 1244, 1413
CseI GACGC 1 cut(s) 314
Csp6I GTAC 1 cut(s) 844
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 9 cut(s) 10, 522, 659, 695, 827, 995, 1160, 1327, 1410
CviQI GTAC 1 cut(s) 844
DdeI CTNAG 5 cut(s) 38, 191, 365, 808, 1015
DpnI GATC 8 cut(s) 162, 357, 570, 823, 900, 1145, 1167, 1335
DpnII GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
DraI TTTAAA 2 cut(s) 543, 703
DraIII CACNNNGTG 1 cut(s) 1396
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
Ecl136II GAGCTC 1 cut(s) 892
Eco24I GRGCYC 1 cut(s) 894
Eco32I GATATC 1 cut(s) 1183
Eco47I GGWCC 2 cut(s) 17, 1244
Eco53kI GAGCTC 1 cut(s) 892
Eco57I CTGAAG 3 cut(s) 673, 1122, 1292
EcoICRI GAGCTC 1 cut(s) 892
EcoO109I RGGNCCY 1 cut(s) 1244
EcoRII CCWGG 2 cut(s) 195, 1288
EcoRV GATATC 1 cut(s) 1183
EcoT22I ATGCAT 2 cut(s) 996, 1411
EcoT38I GRGCYC 1 cut(s) 894
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 9 cut(s) 13, 525, 662, 698, 830, 998, 1163, 1330, 1413
FaqI GGGAC 1 cut(s) 30
FatI CATG 9 cut(s) 9, 521, 658, 694, 826, 994, 1159, 1326, 1409
FbaI TGATCA 1 cut(s) 898
FblI GTMKAC 2 cut(s) 328, 915
Fnu4HI GCNGC 2 cut(s) 600, 813
FokI GGATG 6 cut(s) 185, 194, 263, 560, 635, 1089
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 2 cut(s) 600, 813
FspBI CTAG 3 cut(s) 23, 818, 1417
GlaI GCGC 2 cut(s) 7, 500
GluI GCNGC 2 cut(s) 600, 813
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 3 cut(s) 633, 1325, 1415
HapII CCGG 2 cut(s) 230, 296
HgaI GACGC 1 cut(s) 314
HhaI GCGC 2 cut(s) 8, 501
Hin1I GRCGYC 1 cut(s) 325
Hin1II CATG 9 cut(s) 13, 525, 662, 698, 830, 998, 1163, 1330, 1413
Hin6I GCGC 2 cut(s) 6, 499
HinP1I GCGC 2 cut(s) 6, 499
HincII GTYRAC 1 cut(s) 329
HindII GTYRAC 1 cut(s) 329
HindIII AAGCTT 2 cut(s) 786, 1061
HinfI GANTC 4 cut(s) 87, 104, 385, 532
HpaII CCGG 2 cut(s) 230, 296
HphI GGTGA 6 cut(s) 26, 51, 217, 1079, 1130, 1218
Hpy166II GTNNAC 3 cut(s) 329, 916, 1226
Hpy188I TCNGA 8 cut(s) 76, 118, 240, 355, 885, 969, 1141, 1311
Hpy8I GTNNAC 3 cut(s) 329, 916, 1226
Hpy99I CGWCG 2 cut(s) 113, 330
HpyAV CCTTC 6 cut(s) 7, 107, 141, 613, 1146, 1235
HpyCH4III ACNGT 5 cut(s) 63, 310, 445, 920, 1230
HpyCH4IV ACGT 3 cut(s) 245, 640, 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 991
HpyF3I CTNAG 5 cut(s) 38, 191, 365, 808, 1015
HpySE526I ACGT 3 cut(s) 245, 640, 931
Hsp92I GRCGYC 1 cut(s) 325
Hsp92II CATG 9 cut(s) 13, 525, 662, 698, 830, 998, 1163, 1330, 1413
HspAI GCGC 2 cut(s) 6, 499
Kpn2I TCCGGA 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
LmnI GCTCC 1 cut(s) 889
Lsp1109I GCAGC 2 cut(s) 611, 824
LweI GCATC 4 cut(s) 394, 538, 772, 1111
MaeI CTAG 3 cut(s) 23, 818, 1417
MaeII ACGT 3 cut(s) 245, 640, 931
MaeIII GTNAC 4 cut(s) 508, 1067, 1230, 1292
MalI GATC 8 cut(s) 162, 357, 570, 823, 900, 1145, 1167, 1335
MboI GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
MboII GAAGA 8 cut(s) 119, 126, 196, 472, 1057, 1124, 1177, 1250
MfeI CAATTG 2 cut(s) 408, 1107
MflI RGATCY 4 cut(s) 355, 821, 1143, 1333
MhlI GDGCHC 1 cut(s) 894
MluCI AATT 8 cut(s) 70, 166, 408, 469, 672, 717, 1107, 1274
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 3 cut(s) 99, 141, 1311
Mph1103I ATGCAT 2 cut(s) 996, 1411
MroI TCCGGA 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 30
MseI TTAA 8 cut(s) 249, 312, 528, 542, 702, 791, 1256, 1263
MslI CAYNNNNRTG 3 cut(s) 39, 884, 1017
MspCI CTTAAG 1 cut(s) 1262
MspI CCGG 2 cut(s) 230, 296
MspR9I CCNGG 3 cut(s) 197, 230, 1290
MunI CAATTG 2 cut(s) 408, 1107
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 2 cut(s) 197, 1290
MwoI GCNNNNNNNGC 2 cut(s) 192, 991
NciI CCSGG 1 cut(s) 230
NdeII GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
NlaIII CATG 9 cut(s) 13, 525, 662, 698, 830, 998, 1163, 1330, 1413
NlaIV GGNNCC 4 cut(s) 18, 357, 823, 1335
NmuCI GTSAC 2 cut(s) 1067, 1292
NsiI ATGCAT 2 cut(s) 996, 1411
OliI CACNNNNGTG 1 cut(s) 39
PagI TCATGA 1 cut(s) 658
PctI GAATGC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 30
PfeI GAWTC 3 cut(s) 87, 385, 532
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 2 cut(s) 601, 814
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 1244
PshAI GACNNNNGTC 1 cut(s) 123
PsiI TTATAA 1 cut(s) 1059
Psp124BI GAGCTC 1 cut(s) 894
Psp5II RGGWCCY 1 cut(s) 1244
Psp6I CCWGG 2 cut(s) 195, 1288
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 2 cut(s) 195, 1288
PspN4I GGNNCC 4 cut(s) 18, 357, 823, 1335
PspPI GGNCC 3 cut(s) 17, 1244, 1413
PspPPI RGGWCCY 1 cut(s) 1244
PstI CTGCAG 2 cut(s) 490, 1352
PsuI RGATCY 4 cut(s) 355, 821, 1143, 1333
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 1 cut(s) 845
RsaNI GTAC 1 cut(s) 844
RseI CAYNNNNRTG 3 cut(s) 39, 884, 1017
SacI GAGCTC 1 cut(s) 894
SalI GTCGAC 1 cut(s) 327
SaqAI TTAA 8 cut(s) 249, 312, 528, 542, 702, 791, 1256, 1263
SatI GCNGC 2 cut(s) 600, 813
Sau3AI GATC 8 cut(s) 160, 355, 568, 821, 898, 1143, 1165, 1333
Sau96I GGNCC 3 cut(s) 17, 1244, 1413
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 197, 230, 1290
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 4 cut(s) 394, 538, 772, 1111
SfcI CTRYAG 2 cut(s) 486, 1348
SinI GGWCC 2 cut(s) 17, 1244
SmiMI CAYNNNNRTG 3 cut(s) 39, 884, 1017
SmlI CTYRAG 2 cut(s) 1262, 1319
SmoI CTYRAG 2 cut(s) 1262, 1319
Sse9I AATT 8 cut(s) 70, 166, 408, 469, 672, 717, 1107, 1274
SsiI CCGC 1 cut(s) 457
SspI AATATT 1 cut(s) 835
SspMI CTAG 3 cut(s) 23, 818, 1417
SstI GAGCTC 1 cut(s) 894
StyD4I CCNGG 3 cut(s) 195, 228, 1288
TaaI ACNGT 5 cut(s) 63, 310, 445, 920, 1230
TaiI ACGT 3 cut(s) 248, 643, 934
TaqI TCGA 4 cut(s) 85, 159, 328, 590
TasI AATT 8 cut(s) 70, 166, 408, 469, 672, 717, 1107, 1274
TfiI GAWTC 3 cut(s) 87, 385, 532
Tru1I TTAA 8 cut(s) 249, 312, 528, 542, 702, 791, 1256, 1263
Tru9I TTAA 8 cut(s) 249, 312, 528, 542, 702, 791, 1256, 1263
TscAI CASTG 2 cut(s) 98, 1287
TseFI GTSAC 2 cut(s) 1067, 1292
TseI GCWGC 2 cut(s) 599, 812
Tsp45I GTSAC 2 cut(s) 1067, 1292
TspDTI ATGAA 3 cut(s) 26, 632, 647
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 2 cut(s) 98, 1287
Tth111I GACNNNGTC 1 cut(s) 107
Vha464I CTTAAG 1 cut(s) 1262
VpaK11BI GGWCC 2 cut(s) 17, 1244
XmiI GTMKAC 2 cut(s) 328, 915
XmnI GAANNNNTTC 1 cut(s) 30
XspI CTAG 3 cut(s) 23, 818, 1417
Zsp2I ATGCAT 2 cut(s) 996, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.