Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004584

Physical Location & Seq

Genomic Coordinates Reverse (-)

36926692 .. 36930071

Gene Structure 3380 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1292200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGGAGTCCTGTGATTGCGTAGATGCACAATGGCCCCCGGATGAGTTGCTGGTGAAATATCAGTACATATCTGATGTGTTCATTGCCCTTGCCTATTTCTCCATTCCCTTGGAGCTCGTATATTTTGTGCAGAAATCCGCTTTCTTTCCGTACAGATGGGTGCTTATGCAATTCGGTGCTTTCATTATTCTTTGTGGAGCAACCCATTTCATAAATGTCTGGACTTTCTCTATGCACACCAAGACCGTTGCTATAGTTATGACTGTTGCCAAGGTTTCGTGTGCCGTTGTATCGTGTGCTACCGCTCTAATGCTCGTTCACATTATCCCTGATTTGCTAAGCGTCAAGAGCCGGGAGCTGCGTCTGAAGAACAGAGCTGAAGAGCTTGACAGGGAGATGGGGCTCATGCTCACTCAGGAAGAAACAGGAAGGCATGTTAGAATGCTGACTCATGAAATTCGAAGCACATTGGATAGGGACACTATATTGAAAACTACCCTTGTTGAGTTGGGTAGAACTTTAGGCCTCGAGGAATGCGCGTTGTGGATGCCGTCCAGAACCGGTATGAACCTACAGCTTCATCACACTCTGAACTACCAGATACAAGTTGGGTCTACTGTGTCAATAAACCTTCCTGTGGTCAATGAAGTCTTCAATAGTTCTCGAGCAATTCGTATACCATACACCTGCCCACTGGCCAGGATCAGACCGCTTGTGGGAAGATATGTGCCGCCTGAGGTTGTTGCTCTACGGGTACCCTTATTGAATCTTTCGAATTTTGAAATCAATGACTGGCCGGATCTCTCTGCCAAAAGCTACGCAATCATGGTATTGATTCTACCCACTGATAGCACCAGAAAATGGCGAGACCATGAATTGGAGCTCGTTGATGTTGTCGCAGATCAGGTAGCTGTTGCTCTTTCACATGCTGCTATTATGGAAGAGTCTATGCGAGCCCGTGCTCAGCTCGTGGAGCAGAACAACGCTTTAGATTTAGCTCGGAGAGAAGCAGAGATGGCAATCCATGCTCGCAATGATTTCCTTGCTGTCATGAACCATGAAATGAGGACACCAATGCAGGCAATCATTGCATTGTCTTCTCTTCTGTTAGAGACGGAGCTGACTCCGGAACAAAGGCTTATGCTAGAGACAGTTCTAAAGAGTAGTAACCTTTTGGAAACACTTATTAATGATGTTTTAGATCTTTCTAGACTAGATGATGGCAGTCTAGAATTAGACAATGAAATATTTAACCTCCATGGAGTTTTCAGAGAGGTAATGAATTTGGTACAGCCCATCGCATCTGTGAAGAAGTTGTCTATGACTTTGATTCTAGCCCCAGATTTACCTGTGTGCGCTGTTGGAGATGAGAACCGGCTTATGCAAACCATTCTCAATGTTGCCAGTAATGCAGTGAAGTTCACAAAGCAGGGCTATATTTCAATTATAGTGTCTGTTGCGAAACCAGAATCTTCAAGAGACTGGCAACCACCGGAGTTTTATCCAGCGTCAACTGAGGGCCACTTCTACCTACGAGTTCAGGTTAAGGATTCCGGCTGTGGTGTTCTCCCAGAAGATATTCCTCATGTTTTCACCAAGTTTTCTCAGCCCCGAAATGGATCCAATCGAACAAACGAGGGTGCAGGACTTGGACTTGCCATTTGCAAACGGTTTGTAAACACCATGGGAGGTCATATTTGGATTGAAAGCGAGGGTATCGACAAAGGGAGTATCGTTGCATTCATCGTCAAACTGGGGATATGCGACAATCATAGTGATACAACAACGCACCAGGTCCAGATGCCACCTAAAGGACAAGCAAATTATGGAAGCGGAGATCTCGTTGGACGCAAACCATCCTTTAGGGATCGGTTTGGCTCAAGTTCATCGAATGCACGTTATCAAAGAAGTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

639

Amino Acids

71.55

Weight (kDa)

5.97

Isoelectric Point (pI)

36.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	17 - 115	2.2e-37	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	158 - 307	7e-15	GAF domain
HisKA	PF00512	343 - 408	3.9e-18	His Kinase A (phospho-acceptor) domain
HATPase_c	PF02518	455 - 585	1.5e-23	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40940
fragaria_vesca	FvH4_2g26120 FvH4_2g26120 FvH4_2g26120
malus_domestica	MD03G1292200.v1.1 MD06G1001100.v1.1 MD11G1306200.v1.1
prunus_persica	Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1 Prupe.8G265200_v2.0.a1
pyrus_communis	pycom03g23140 pycom11g26800
rosa_chinensis	RchiOBHm_Chr6g0294491
rosa_laevigata	RLG00000011864
rosa_roxburghii	Rroxscaffold_7G00173280
rosa_rugosa	Rorug06G0245200 Rorug06G0245300 Rorug06G0245400
rosa_samantha	Rh6AG357700 Rh6BG364600 Rh6CG371700 Rh6DG358500
rosa_wichuraiana	Rw6G031200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	695
AbsI	CCTCGAGG	1 cut(s)	527
Acc36I	ACCTGC	1 cut(s)	695
Acc65I	GGTACC	1 cut(s)	754
AccB1I	GGYRCC	1 cut(s)	754
AccB7I	CCANNNNNTGG	2 cut(s)	861, 877
AccBSI	CCGCTC	1 cut(s)	305
AccI	GTMKAC	2 cut(s)	614, 676
AccII	CGCG	1 cut(s)	539
AccIII	TCCGGA	1 cut(s)	1126
AciI	CCGC	5 cut(s)	138, 303, 710, 731, 1833
AclWI	GGATC	5 cut(s)	710, 807, 1614, 1627, 1875
AcoI	YGGCCR	2 cut(s)	696, 794
AcsI	RAATTY	3 cut(s)	456, 775, 1282
AcuI	CTGAAG	2 cut(s)	386, 399
AfaI	GTAC	4 cut(s)	65, 152, 756, 1290
AfiI	CCNNNNNNNGG	6 cut(s)	637, 716, 861, 877, 1616, 1811
AgeI	ACCGGT	1 cut(s)	560
AgsI	TTSAA	7 cut(s)	490, 655, 766, 782, 1443, 1476, 1706
AjnI	CCWGG	2 cut(s)	698, 1791
Alw21I	GWGCWC	3 cut(s)	117, 885, 964
Alw26I	GTCTC	4 cut(s)	861, 1106, 1142, 1473
AlwI	GGATC	5 cut(s)	710, 807, 1614, 1627, 1875
Ama87I	CYCGRG	2 cut(s)	527, 663
Aor13HI	TCCGGA	1 cut(s)	1126
AoxI	GGCC	5 cut(s)	32, 523, 696, 794, 1519
ApeKI	GCWGC	2 cut(s)	358, 929
ApoI	RAATTY	3 cut(s)	456, 775, 1282
ArsI	GACNNNNNNTTYG	2 cut(s)	1644, 1676
AseI	ATTAAT	1 cut(s)	1188
AsiGI	ACCGGT	1 cut(s)	560
Asp700I	GAANNNNTTC	1 cut(s)	1578
Asp718I	GGTACC	1 cut(s)	754
AspLEI	GCGC	2 cut(s)	539, 1358
AspS9I	GGNCC	3 cut(s)	33, 1519, 1795
AsuC2I	CCSGG	2 cut(s)	38, 353
AsuHPI	GGTGA	2 cut(s)	64, 1585
AsuII	TTCGAA	2 cut(s)	460, 773
AvaI	CYCGRG	2 cut(s)	527, 663
AvaII	GGWCC	1 cut(s)	1795
AxyI	CCTNAGG	1 cut(s)	735
BalI	TGGCCA	1 cut(s)	698
BamHI	GGATCC	1 cut(s)	1619
BanI	GGYRCC	1 cut(s)	754
BanII	GRGCYC	4 cut(s)	117, 405, 885, 958
BauI	CACGAG	1 cut(s)	968
BbsI	GAAGAC	2 cut(s)	643, 1089
Bbv12I	GWGCWC	3 cut(s)	117, 885, 964
BbvI	GCAGC	2 cut(s)	345, 916
BccI	CCATC	6 cut(s)	150, 391, 1009, 1214, 1304, 1864
BceAI	ACGGC	2 cut(s)	269, 535
BciT130I	CCWGG	2 cut(s)	700, 1793
BcnI	CCSGG	2 cut(s)	38, 353
BcoDI	GTCTC	4 cut(s)	861, 1106, 1142, 1473
BfaI	CTAG	5 cut(s)	1145, 1209, 1214, 1229, 1334
BfmI	CTRYAG	2 cut(s)	252, 572
BfuAI	ACCTGC	1 cut(s)	695
BglII	AGATCT	2 cut(s)	1201, 1837
BisI	GCNGC	3 cut(s)	359, 731, 930
BlpI	GCTNAGC	2 cut(s)	338, 963
BlsI	GCNGC	3 cut(s)	360, 732, 931
Bme1390I	CCNGG	4 cut(s)	38, 353, 700, 1793
Bme18I	GGWCC	1 cut(s)	1795
BmeT110I	CYCGRG	2 cut(s)	527, 663
BmgT120I	GGNCC	3 cut(s)	33, 1519, 1795
BmiI	GGNNCC	3 cut(s)	35, 756, 1621
BmrFI	CCNGG	4 cut(s)	38, 353, 700, 1793
BmrI	ACTGGG	1 cut(s)	1763
BmsI	GCATC	4 cut(s)	13, 537, 1310, 1791
BmuI	ACTGGG	1 cut(s)	1763
BpiI	GAAGAC	2 cut(s)	643, 1089
Bpu1102I	GCTNAGC	2 cut(s)	338, 963
Bpu14I	TTCGAA	2 cut(s)	460, 773
BpuEI	CTTGAG	1 cut(s)	1864
BpuMI	CCSGG	2 cut(s)	38, 353
BsaBI	GATNNNNATC	1 cut(s)	1019
BsaI	GGTCTC	1 cut(s)	861
BsaJI	CCNNGG	5 cut(s)	36, 108, 270, 1258, 1683
BsaWI	WCCGGW	3 cut(s)	560, 1126, 1492
BsaXI	ACNNNNNCTCC	2 cut(s)	1827, 1857
Bsc4I	CCNNNNNNNGG	6 cut(s)	637, 716, 861, 877, 1616, 1811
Bse118I	RCCGGY	2 cut(s)	560, 1374
Bse1I	ACTGG	5 cut(s)	699, 797, 1404, 1487, 1758
Bse21I	CCTNAGG	1 cut(s)	735
Bse3DI	GCAATG	3 cut(s)	81, 1039, 1086
Bse8I	GATNNNNATC	1 cut(s)	1019
BseAI	TCCGGA	1 cut(s)	1126
BseBI	CCWGG	2 cut(s)	700, 1793
BseDI	CCNNGG	5 cut(s)	36, 108, 270, 1258, 1683
BseGI	GGATG	3 cut(s)	46, 552, 1856
BseJI	GATNNNNATC	1 cut(s)	1019
BseLI	CCNNNNNNNGG	6 cut(s)	637, 716, 861, 877, 1616, 1811
BseMI	GCAATG	3 cut(s)	81, 1039, 1086
BseMII	CTCAG	5 cut(s)	428, 726, 977, 1506, 1619
BseNI	ACTGG	5 cut(s)	699, 797, 1404, 1487, 1758
BseXI	GCAGC	2 cut(s)	345, 916
BsgI	GTGCAG	2 cut(s)	149, 1662
Bsh1236I	CGCG	1 cut(s)	539
BshFI	GGCC	5 cut(s)	34, 525, 698, 796, 1521
BshNI	GGYRCC	1 cut(s)	754
BshTI	ACCGGT	1 cut(s)	560
BsiHKAI	GWGCWC	3 cut(s)	117, 885, 964
BsiHKCI	CYCGRG	2 cut(s)	527, 663
BsiSI	CCGG	8 cut(s)	38, 352, 561, 797, 1127, 1375, 1493, 1554
BslFI	GGGAC	1 cut(s)	491
BslI	CCNNNNNNNGG	6 cut(s)	637, 716, 861, 877, 1616, 1811
BsmAI	GTCTC	4 cut(s)	861, 1106, 1142, 1473
BsmBI	CGTCTC	1 cut(s)	1106
BsmFI	GGGAC	1 cut(s)	491
BsmI	GAATGC	4 cut(s)	447, 539, 1739, 1897
BsnI	GGCC	5 cut(s)	34, 525, 698, 796, 1521
Bso31I	GGTCTC	1 cut(s)	861
BsoBI	CYCGRG	2 cut(s)	527, 663
Bsp119I	TTCGAA	2 cut(s)	460, 773
Bsp1286I	GDGCHC	5 cut(s)	117, 405, 885, 958, 964
Bsp13I	TCCGGA	1 cut(s)	1126
Bsp143I	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
Bsp1720I	GCTNAGC	2 cut(s)	338, 963
Bsp19I	CCATGG	2 cut(s)	1258, 1683
BspACI	CCGC	5 cut(s)	138, 303, 710, 731, 1833
BspANI	GGCC	5 cut(s)	34, 525, 698, 796, 1521
BspCNI	CTCAG	5 cut(s)	427, 727, 976, 1507, 1618
BspEI	TCCGGA	1 cut(s)	1126
BspFNI	CGCG	1 cut(s)	539
BspHI	TCATGA	2 cut(s)	451, 1050
BspLI	GGNNCC	3 cut(s)	35, 756, 1621
BspMI	ACCTGC	1 cut(s)	695
BspPI	GGATC	5 cut(s)	710, 807, 1614, 1627, 1875
BspQI	GCTCTTC	1 cut(s)	375
BspT104I	TTCGAA	2 cut(s)	460, 773
BspT107I	GGYRCC	1 cut(s)	754
BspTNI	GGTCTC	1 cut(s)	861
BsrBI	CCGCTC	1 cut(s)	305
BsrDI	GCAATG	3 cut(s)	81, 1039, 1086
BsrFI	RCCGGY	2 cut(s)	560, 1374
BsrI	ACTGG	5 cut(s)	699, 797, 1404, 1487, 1758
BssAI	RCCGGY	2 cut(s)	560, 1374
BssECI	CCNNGG	5 cut(s)	36, 108, 270, 1258, 1683
BssMI	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
BssNAI	GTATAC	1 cut(s)	677
BssSI	CACGAG	1 cut(s)	968
BssT1I	CCWWGG	4 cut(s)	108, 270, 1258, 1683
Bst1107I	GTATAC	1 cut(s)	677
Bst2BI	CACGAG	1 cut(s)	968
Bst2UI	CCWGG	2 cut(s)	700, 1793
Bst4CI	ACNGT	5 cut(s)	247, 265, 619, 1153, 1671
Bst6I	CTCTTC	3 cut(s)	375, 936, 1107
BstAPI	GCANNNNNTGC	2 cut(s)	1025, 1088
BstBI	TTCGAA	2 cut(s)	460, 773
BstC8I	GCNNGC	3 cut(s)	954, 1030, 1080
BstDEI	CTNAG	6 cut(s)	338, 414, 735, 963, 1515, 1605
BstDSI	CCRYGG	2 cut(s)	1258, 1683
BstF5I	GGATG	3 cut(s)	46, 552, 1856
BstFNI	CGCG	1 cut(s)	539
BstHHI	GCGC	2 cut(s)	539, 1358
BstKTI	GATC	7 cut(s)	705, 802, 904, 1204, 1622, 1840, 1870
BstMAI	GTCTC	4 cut(s)	861, 1106, 1142, 1473
BstMBI	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
BstMWI	GCNNNNNNNGC	6 cut(s)	348, 973, 1016, 1025, 1088, 1409
BstNI	CCWGG	2 cut(s)	700, 1793
BstNSI	RCATGY	2 cut(s)	437, 929
BstSCI	CCNGG	4 cut(s)	36, 351, 698, 1791
BstSFI	CTRYAG	2 cut(s)	252, 572
BstUI	CGCG	1 cut(s)	539
BstV1I	GCAGC	2 cut(s)	345, 916
BstV2I	GAAGAC	2 cut(s)	643, 1089
BstX2I	RGATCY	4 cut(s)	799, 1201, 1619, 1837
BstXI	CCANNNNNNTGG	1 cut(s)	109
BstYI	RGATCY	4 cut(s)	799, 1201, 1619, 1837
BstZ17I	GTATAC	1 cut(s)	677
Bsu36I	CCTNAGG	1 cut(s)	735
BsuRI	GGCC	5 cut(s)	34, 525, 698, 796, 1521
BtgI	CCRYGG	2 cut(s)	1258, 1683
BtgZI	GCGATG	1 cut(s)	1282
BtsCI	GGATG	3 cut(s)	46, 552, 1856
BtsI	GCAGTG	1 cut(s)	1419
BtsIMutI	CAGTG	3 cut(s)	692, 843, 1419
BveI	ACCTGC	1 cut(s)	695
Cac8I	GCNNGC	3 cut(s)	954, 1030, 1080
CciI	TCATGA	2 cut(s)	451, 1050
CfoI	GCGC	2 cut(s)	539, 1358
Cfr10I	RCCGGY	2 cut(s)	560, 1374
Cfr13I	GGNCC	3 cut(s)	33, 1519, 1795
CseI	GACGC	4 cut(s)	331, 350, 1497, 1857
CsiI	ACCWGGT	1 cut(s)	1791
Csp6I	GTAC	4 cut(s)	64, 151, 755, 1289
CspAI	ACCGGT	1 cut(s)	560
CviQI	GTAC	4 cut(s)	64, 151, 755, 1289
DdeI	CTNAG	6 cut(s)	338, 414, 735, 963, 1515, 1605
DpnI	GATC	7 cut(s)	704, 801, 903, 1203, 1621, 1839, 1869
DpnII	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
EaeI	YGGCCR	2 cut(s)	696, 794
Eam1104I	CTCTTC	3 cut(s)	375, 936, 1107
EarI	CTCTTC	3 cut(s)	375, 936, 1107
Ecl136II	GAGCTC	2 cut(s)	115, 883
Eco130I	CCWWGG	4 cut(s)	108, 270, 1258, 1683
Eco147I	AGGCCT	1 cut(s)	525
Eco24I	GRGCYC	4 cut(s)	117, 405, 885, 958
Eco31I	GGTCTC	1 cut(s)	861
Eco47I	GGWCC	1 cut(s)	1795
Eco53kI	GAGCTC	2 cut(s)	115, 883
Eco57I	CTGAAG	2 cut(s)	386, 399
Eco81I	CCTNAGG	1 cut(s)	735
Eco88I	CYCGRG	2 cut(s)	527, 663
EcoICRI	GAGCTC	2 cut(s)	115, 883
EcoRII	CCWGG	2 cut(s)	698, 1791
EcoT14I	CCWWGG	4 cut(s)	108, 270, 1258, 1683
EcoT38I	GRGCYC	4 cut(s)	117, 405, 885, 958
ErhI	CCWWGG	4 cut(s)	108, 270, 1258, 1683
Esp3I	CGTCTC	1 cut(s)	1106
FaqI	GGGAC	1 cut(s)	491
FblI	GTMKAC	2 cut(s)	614, 676
Fnu4HI	GCNGC	3 cut(s)	359, 731, 930
FokI	GGATG	3 cut(s)	53, 559, 1843
FriOI	GRGCYC	4 cut(s)	117, 405, 885, 958
Fsp4HI	GCNGC	3 cut(s)	359, 731, 930
FspBI	CTAG	5 cut(s)	1145, 1209, 1214, 1229, 1334
GlaI	GCGC	2 cut(s)	538, 1357
GluI	GCNGC	3 cut(s)	359, 731, 930
HaeIII	GGCC	5 cut(s)	34, 525, 698, 796, 1521
HapII	CCGG	8 cut(s)	38, 352, 561, 797, 1127, 1375, 1493, 1554
HgaI	GACGC	4 cut(s)	331, 350, 1497, 1857
HhaI	GCGC	2 cut(s)	539, 1358
Hin6I	GCGC	2 cut(s)	537, 1356
HinP1I	GCGC	2 cut(s)	537, 1356
HincII	GTYRAC	1 cut(s)	1512
HindII	GTYRAC	1 cut(s)	1512
HinfI	GANTC	9 cut(s)	5, 448, 766, 835, 944, 1123, 1330, 1469, 1550
HpaII	CCGG	8 cut(s)	38, 352, 561, 797, 1127, 1375, 1493, 1554
HphI	GGTGA	2 cut(s)	64, 1585
Hpy166II	GTNNAC	6 cut(s)	319, 615, 677, 1422, 1512, 1678
Hpy188I	TCNGA	6 cut(s)	73, 366, 591, 707, 1002, 1271
Hpy8I	GTNNAC	6 cut(s)	319, 615, 677, 1422, 1512, 1678
HpyAV	CCTTC	2 cut(s)	423, 641
HpyCH4III	ACNGT	5 cut(s)	247, 265, 619, 1153, 1671
HpyCH4IV	ACGT	1 cut(s)	1897
HpyF10VI	GCNNNNNNNGC	6 cut(s)	348, 973, 1016, 1025, 1088, 1409
HpyF3I	CTNAG	6 cut(s)	338, 414, 735, 963, 1515, 1605
HpySE526I	ACGT	1 cut(s)	1897
HspAI	GCGC	2 cut(s)	537, 1356
Kpn2I	TCCGGA	1 cut(s)	1126
KpnI	GGTACC	1 cut(s)	758
Kzo9I	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
LguI	GCTCTTC	1 cut(s)	375
LmnI	GCTCC	6 cut(s)	112, 197, 355, 880, 973, 1117
Lsp1109I	GCAGC	2 cut(s)	345, 916
LweI	GCATC	4 cut(s)	13, 537, 1310, 1791
MabI	ACCWGGT	1 cut(s)	1791
MaeI	CTAG	5 cut(s)	1145, 1209, 1214, 1229, 1334
MaeII	ACGT	1 cut(s)	1897
MaeIII	GTNAC	1 cut(s)	1166
MalI	GATC	7 cut(s)	704, 801, 903, 1203, 1621, 1839, 1869
MbiI	CCGCTC	1 cut(s)	305
MboI	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
MflI	RGATCY	4 cut(s)	799, 1201, 1619, 1837
MhlI	GDGCHC	5 cut(s)	117, 405, 885, 958, 964
MlsI	TGGCCA	1 cut(s)	698
MluCI	AATT	9 cut(s)	170, 456, 669, 775, 875, 1232, 1282, 1443, 1822
MluNI	TGGCCA	1 cut(s)	698
MlyI	GAGTC	4 cut(s)	14, 442, 953, 1117
MmeI	TCCRAC	2 cut(s)	1342, 1825
Mox20I	TGGCCA	1 cut(s)	698
MroI	TCCGGA	1 cut(s)	1126
MroXI	GAANNNNTTC	1 cut(s)	1578
MscI	TGGCCA	1 cut(s)	698
MseI	TTAA	3 cut(s)	1188, 1251, 1545
Msp20I	TGGCCA	1 cut(s)	698
MspI	CCGG	8 cut(s)	38, 352, 561, 797, 1127, 1375, 1493, 1554
MspR9I	CCNGG	4 cut(s)	38, 353, 700, 1793
Mva1269I	GAATGC	4 cut(s)	447, 539, 1739, 1897
MvaI	CCWGG	2 cut(s)	700, 1793
MvnI	CGCG	1 cut(s)	539
MwoI	GCNNNNNNNGC	6 cut(s)	348, 973, 1016, 1025, 1088, 1409
NciI	CCSGG	2 cut(s)	38, 353
NcoI	CCATGG	2 cut(s)	1258, 1683
NdeII	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
NlaIV	GGNNCC	3 cut(s)	35, 756, 1621
NspI	RCATGY	2 cut(s)	437, 929
NspV	TTCGAA	2 cut(s)	460, 773
PaeR7I	CTCGAG	2 cut(s)	527, 663
PagI	TCATGA	2 cut(s)	451, 1050
PaqCI	CACCTGC	1 cut(s)	695
PceI	AGGCCT	1 cut(s)	525
PciSI	GCTCTTC	1 cut(s)	375
PcsI	WCGNNNNNNNCGW	1 cut(s)	670
PctI	GAATGC	4 cut(s)	447, 539, 1739, 1897
PdmI	GAANNNNTTC	1 cut(s)	1578
PfeI	GAWTC	5 cut(s)	766, 835, 1330, 1469, 1550
PflMI	CCANNNNNTGG	2 cut(s)	861, 877
PinAI	ACCGGT	1 cut(s)	560
PkrI	GCNGC	3 cut(s)	360, 732, 931
PleI	GAGTC	4 cut(s)	13, 442, 952, 1117
PpsI	GAGTC	4 cut(s)	13, 442, 952, 1117
PshBI	ATTAAT	1 cut(s)	1188
Psp124BI	GAGCTC	2 cut(s)	117, 885
Psp6I	CCWGG	2 cut(s)	698, 1791
PspGI	CCWGG	2 cut(s)	698, 1791
PspN4I	GGNNCC	3 cut(s)	35, 756, 1621
PspPI	GGNCC	3 cut(s)	33, 1519, 1795
PspXI	VCTCGAGB	1 cut(s)	527
PsuI	RGATCY	4 cut(s)	799, 1201, 1619, 1837
RsaI	GTAC	4 cut(s)	65, 152, 756, 1290
RsaNI	GTAC	4 cut(s)	64, 151, 755, 1289
SacI	GAGCTC	2 cut(s)	117, 885
SapI	GCTCTTC	1 cut(s)	375
SaqAI	TTAA	3 cut(s)	1188, 1251, 1545
SatI	GCNGC	3 cut(s)	359, 731, 930
Sau3AI	GATC	7 cut(s)	702, 799, 901, 1201, 1619, 1837, 1867
Sau96I	GGNCC	3 cut(s)	33, 1519, 1795
SchI	GAGTC	4 cut(s)	14, 442, 953, 1117
ScrFI	CCNGG	4 cut(s)	38, 353, 700, 1793
SduI	GDGCHC	5 cut(s)	117, 405, 885, 958, 964
SexAI	ACCWGGT	1 cut(s)	1791
SfaNI	GCATC	4 cut(s)	13, 537, 1310, 1791
SfcI	CTRYAG	2 cut(s)	252, 572
Sfr274I	CTCGAG	2 cut(s)	527, 663
SfuI	TTCGAA	2 cut(s)	460, 773
SinI	GGWCC	1 cut(s)	1795
SlaI	CTCGAG	2 cut(s)	527, 663
SmlI	CTYRAG	3 cut(s)	527, 663, 1879
SmoI	CTYRAG	3 cut(s)	527, 663, 1879
Sse9I	AATT	9 cut(s)	170, 456, 669, 775, 875, 1232, 1282, 1443, 1822
SseBI	AGGCCT	1 cut(s)	525
SsiI	CCGC	5 cut(s)	138, 303, 710, 731, 1833
SspI	AATATT	1 cut(s)	1248
SspMI	CTAG	5 cut(s)	1145, 1209, 1214, 1229, 1334
SstI	GAGCTC	2 cut(s)	117, 885
StuI	AGGCCT	1 cut(s)	525
StyD4I	CCNGG	4 cut(s)	36, 351, 698, 1791
StyI	CCWWGG	4 cut(s)	108, 270, 1258, 1683
TaaI	ACNGT	5 cut(s)	247, 265, 619, 1153, 1671
TaiI	ACGT	1 cut(s)	1900
TaqI	TCGA	7 cut(s)	460, 528, 664, 773, 1627, 1719, 1889
TasI	AATT	9 cut(s)	170, 456, 669, 775, 875, 1232, 1282, 1443, 1822
TatI	WGTACW	1 cut(s)	63
TauI	GCSGC	1 cut(s)	733
TfiI	GAWTC	5 cut(s)	766, 835, 1330, 1469, 1550
Tru1I	TTAA	3 cut(s)	1188, 1251, 1545
Tru9I	TTAA	3 cut(s)	1188, 1251, 1545
TscAI	CASTG	3 cut(s)	699, 850, 1419
TseI	GCWGC	2 cut(s)	358, 929
TspGWI	ACGGA	2 cut(s)	138, 1130
TspRI	CASTG	3 cut(s)	699, 850, 1419
Van91I	CCANNNNNTGG	2 cut(s)	861, 877
VpaK11BI	GGWCC	1 cut(s)	1795
VspI	ATTAAT	1 cut(s)	1188
XapI	RAATTY	3 cut(s)	456, 775, 1282
XbaI	TCTAGA	2 cut(s)	1208, 1228
XceI	RCATGY	2 cut(s)	437, 929
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XhoI	CTCGAG	2 cut(s)	527, 663
XmiI	GTMKAC	2 cut(s)	614, 676
XmnI	GAANNNNTTC	1 cut(s)	1578
XspI	CTAG	5 cut(s)	1145, 1209, 1214, 1229, 1334

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.