MD06G1001100.v1.1

Ethylene response sensor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004584
Physical Location & Seq
Forward (+)
157782 .. 159580
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1001100.v1.1.491

Sequence Viewer

Length: 1674 bp
ATGGAGTCCTGTGATTGCATAGACGCGCAATGGCCCCCAGATGAGTTGCTGGTGAAATATCAATACATCTCTGATGTGTTCATTGCCCTTGCCTATTTCTCCATTCCCTTGGAGCTCCTATATTTTGTGCAGAAATCCGCTTTCTTTCCGTACAGATGGGTGCTTGTGCAGTTCGGTGCCTTCATTGTTCTTTGTGGAGCAACCCATTTCATAAATCGTCAAGAAACGGGAGCTGTCGCTGAAGAGCTTGACAAGGAGATGGGGTTTATGCTCACTCAGGAAGAAACAGGAAGGCATGTTAGGATGCTGACTCATGAAATTCGAAGCACGTTGGATAGGCACACTATATTGAAAACTACCCTTGTTGAGTTGGGTAGAACTCTAGGCCTCCAGGAATGCGCGTTATGGATGCCGTCTAGAACTGGTATGAACCTACAGCTTTCTCACACTCTGAACTACCACATACAAGCTGGTTCATCTGTGTCAATTGACCTTCATGTTGTCAACGAAGTCTTCAATAGTTCTCGGGCAATTCGTATACCATATACCTGCCCACTGGCAAGAATCAGACCGCTTGTGGGAAGATATGTACCACCTGAGGTTGTTGCTCTGCGGGTGCCCCTTTTAAATCTTTCAAATCTTCAAATCAATGATTGGCCGGATGTCTCTGCCAAAAGCTACGCAATCATGGTTTTGATTCTTCCCACGGATGGCACCAGAAAGTGGCGAGAACATGAACTGGAGCTGGTTGATGTTGTGGCAGATCAGGTAGCTGTTGCTCTTTCACATGCTGCTATTTTGGAAGAGTCTATGCGAGCCCGTAAACAGCTCACGGAGCAGAACGATGCTTTAGATTTAGCTTGGAGAGAAACAGAGATGGCAATCCATACCCGCGATGATTTCCTTGCTGTCATGAACCGTGGAATGAGGACACCAATGCATGCAATCATTGCATTGTCTTCTCTTCTGTTAGAGACGGAACTGACTCCAGAAGAAAGGCTTATGATAGAGACAGCACTAAAGAGTAGCAACCTTTTGGAAACACTTGTTAATGATGTGTTAGATCTTTCTAGACTTGAAGATGATAGCCTAGAATTAGACACTGAAAGATTTAACCTCCATGGAGTTTTCAGAGAGGTAATGAATTTGGTACAGCCCGTCGCATCTATGAAGAAGTTGTCTATGACTTTGATTCAATCCCCAGACTTACTTGTGTATGCCGTTGGAGATGAGAATCGGCTGTTGCAAACTATTCTCAACGTTGCCGGTAATGCTGTGAAGTTCACAAAGCAGGGCTATATTTCAATTACAGCATCTGTTGCGAAATCAGAATCTTCAAGAGACTGGCAACCACCGGAGTTTTATCCAGCGTCAACTGATGGTTACTTCTACCTACGAGTTCAGGTTAAGGATTCTGGCTGTGGGGTTCTCCCAGAAGATATTCCTCATCTTTTCACCAAGTTTTCTCAGCCCCGAAATGGATCCAATCAAATAAACAACGGTGCAGGACTTGGACTTGCCATTTGCAAACGGTTTGTAAACACCATGGGAGGTCATATTTGGATTGAAAGCGAGGGTATCGACAGAGGGAGTATCGTTGCATTCATCGTCCAACTAGGGATATGCGACAATCATAGTGATACAACAGGCCACCAGGTCCAAATAGCCACCTAA

Protein Analysis

558

Amino Acids

62.63

Weight (kDa)

5.27

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 17 - 75 3.6e-24 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 112 - 261 2.6e-15 GAF domain
HisKA PF00512 298 - 361 3.1e-13 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 409 - 539 8.1e-22 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 557
AccB1I GGYRCC 3 cut(s) 176, 616, 713
AccB7I CCANNNNNTGG 1 cut(s) 723
AccI GTMKAC 1 cut(s) 538
AccII CGCG 3 cut(s) 26, 401, 894
AciI CCGC 4 cut(s) 138, 572, 613, 892
AclI AACGTT 1 cut(s) 1260
AclWI GGATC 2 cut(s) 1476, 1489
AcoI YGGCCR 1 cut(s) 656
AcsI RAATTY 2 cut(s) 318, 1144
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 3 cut(s) 152, 591, 1152
AfiI CCNNNNNNNGG 4 cut(s) 578, 710, 723, 1478
AgsI TTSAA 9 cut(s) 352, 517, 636, 644, 1079, 1196, 1305, 1338, 1568
AjnI CCWGG 2 cut(s) 390, 1653
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 4 cut(s) 670, 968, 1004, 1335
AlwI GGATC 2 cut(s) 1476, 1489
AlwNI CAGNNNCTG 1 cut(s) 1316
Ama87I CYCGRG 1 cut(s) 525
AoxI GGCC 4 cut(s) 32, 385, 656, 1648
ApeKI GCWGC 1 cut(s) 791
ApoI RAATTY 2 cut(s) 318, 1144
ArsI GACNNNNNNTTYG 2 cut(s) 1506, 1538
Asp700I GAANNNNTTC 1 cut(s) 1440
AspLEI GCGC 2 cut(s) 28, 401
AspS9I GGNCC 2 cut(s) 33, 1657
AsuHPI GGTGA 2 cut(s) 64, 1447
AsuII TTCGAA 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 525
AvaII GGWCC 1 cut(s) 1657
AxyI CCTNAGG 1 cut(s) 597
BaeGI GKGCMC 1 cut(s) 621
BamHI GGATCC 1 cut(s) 1481
BanI GGYRCC 3 cut(s) 176, 616, 713
BanII GRGCYC 2 cut(s) 117, 820
BbsI GAAGAC 2 cut(s) 505, 951
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 778
BccI CCATC 5 cut(s) 150, 253, 704, 871, 1373
BceAI ACGGC 2 cut(s) 397, 1205
BciT130I CCWGG 2 cut(s) 392, 1655
BcoDI GTCTC 4 cut(s) 670, 968, 1004, 1335
BfaI CTAG 5 cut(s) 383, 417, 1071, 1091, 1616
BfmI CTRYAG 1 cut(s) 434
BfuAI ACCTGC 1 cut(s) 557
BglII AGATCT 1 cut(s) 1063
BisI GCNGC 1 cut(s) 792
BlsI GCNGC 1 cut(s) 793
Bme1390I CCNGG 2 cut(s) 392, 1655
Bme18I GGWCC 1 cut(s) 1657
BmeT110I CYCGRG 1 cut(s) 525
BmgT120I GGNCC 2 cut(s) 33, 1657
BmiI GGNNCC 5 cut(s) 35, 178, 618, 715, 1483
BmrFI CCNGG 2 cut(s) 392, 1655
BmsI GCATC 5 cut(s) 294, 399, 835, 1172, 1322
BpiI GAAGAC 2 cut(s) 505, 951
BpmI CTGGAG 3 cut(s) 374, 761, 972
Bpu14I TTCGAA 1 cut(s) 322
BsaBI GATNNNNATC 2 cut(s) 881, 1233
BsaJI CCNNGG 5 cut(s) 108, 705, 919, 1120, 1545
BsaWI WCCGGW 1 cut(s) 1354
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 4 cut(s) 578, 710, 723, 1478
Bse118I RCCGGY 1 cut(s) 1265
Bse1I ACTGG 4 cut(s) 427, 561, 744, 1349
Bse21I CCTNAGG 1 cut(s) 597
Bse3DI GCAATG 3 cut(s) 35, 81, 948
Bse8I GATNNNNATC 2 cut(s) 881, 1233
BseBI CCWGG 2 cut(s) 392, 1655
BseDI CCNNGG 5 cut(s) 108, 705, 919, 1120, 1545
BseGI GGATG 4 cut(s) 309, 414, 667, 715
BseJI GATNNNNATC 2 cut(s) 881, 1233
BseLI CCNNNNNNNGG 4 cut(s) 578, 710, 723, 1478
BseMI GCAATG 3 cut(s) 35, 81, 948
BseMII CTCAG 3 cut(s) 290, 588, 1481
BseNI ACTGG 4 cut(s) 427, 561, 744, 1349
BseSI GKGCMC 1 cut(s) 621
BseXI GCAGC 1 cut(s) 778
BsgI GTGCAG 3 cut(s) 149, 188, 1524
Bsh1236I CGCG 3 cut(s) 26, 401, 894
BshFI GGCC 4 cut(s) 34, 387, 658, 1650
BshNI GGYRCC 3 cut(s) 176, 616, 713
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 525
BsiSI CCGG 3 cut(s) 659, 1266, 1355
BslI CCNNNNNNNGG 4 cut(s) 578, 710, 723, 1478
BsmAI GTCTC 4 cut(s) 670, 968, 1004, 1335
BsmBI CGTCTC 1 cut(s) 968
BsmI GAATGC 2 cut(s) 401, 1601
BsnI GGCC 4 cut(s) 34, 387, 658, 1650
BsoBI CYCGRG 1 cut(s) 525
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 3 cut(s) 117, 621, 820
Bsp143I GATC 3 cut(s) 763, 1063, 1481
Bsp19I CCATGG 2 cut(s) 1120, 1545
BspACI CCGC 4 cut(s) 138, 572, 613, 892
BspANI GGCC 4 cut(s) 34, 387, 658, 1650
BspCNI CTCAG 3 cut(s) 289, 589, 1480
BspFNI CGCG 3 cut(s) 26, 401, 894
BspHI TCATGA 2 cut(s) 313, 912
BspLI GGNNCC 5 cut(s) 35, 178, 618, 715, 1483
BspMI ACCTGC 1 cut(s) 557
BspPI GGATC 2 cut(s) 1476, 1489
BspQI GCTCTTC 1 cut(s) 237
BspT104I TTCGAA 1 cut(s) 322
BspT107I GGYRCC 3 cut(s) 176, 616, 713
BsrDI GCAATG 3 cut(s) 35, 81, 948
BsrFI RCCGGY 1 cut(s) 1265
BsrI ACTGG 4 cut(s) 427, 561, 744, 1349
BssAI RCCGGY 1 cut(s) 1265
BssECI CCNNGG 5 cut(s) 108, 705, 919, 1120, 1545
BssMI GATC 3 cut(s) 763, 1063, 1481
BssNAI GTATAC 1 cut(s) 539
BssT1I CCWWGG 3 cut(s) 108, 1120, 1545
Bst1107I GTATAC 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 392, 1655
Bst4CI ACNGT 3 cut(s) 920, 1502, 1533
Bst6I CTCTTC 3 cut(s) 237, 798, 969
BstAPI GCANNNNNTGC 2 cut(s) 950, 1319
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 816, 942
BstDEI CTNAG 3 cut(s) 276, 597, 1467
BstDSI CCRYGG 4 cut(s) 705, 919, 1120, 1545
BstF5I GGATG 4 cut(s) 309, 414, 667, 715
BstFNI CGCG 3 cut(s) 26, 401, 894
BstHHI GCGC 2 cut(s) 28, 401
BstKTI GATC 3 cut(s) 766, 1066, 1484
BstMAI GTCTC 4 cut(s) 670, 968, 1004, 1335
BstMBI GATC 3 cut(s) 763, 1063, 1481
BstMWI GCNNNNNNNGC 4 cut(s) 835, 950, 1271, 1319
BstNI CCWGG 2 cut(s) 392, 1655
BstNSI RCATGY 3 cut(s) 299, 791, 944
BstSCI CCNGG 2 cut(s) 390, 1653
BstSFI CTRYAG 1 cut(s) 434
BstSLI GKGCMC 1 cut(s) 621
BstUI CGCG 3 cut(s) 26, 401, 894
BstV1I GCAGC 1 cut(s) 778
BstV2I GAAGAC 2 cut(s) 505, 951
BstX2I RGATCY 2 cut(s) 1063, 1481
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 2 cut(s) 1063, 1481
BstZ17I GTATAC 1 cut(s) 539
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 4 cut(s) 34, 387, 658, 1650
BtgI CCRYGG 4 cut(s) 705, 919, 1120, 1545
BtgZI GCGATG 1 cut(s) 909
BtsCI GGATG 4 cut(s) 309, 414, 667, 715
BtsIMutI CAGTG 2 cut(s) 554, 1101
BveI ACCTGC 1 cut(s) 557
Cac8I GCNNGC 2 cut(s) 816, 942
CaiI CAGNNNCTG 1 cut(s) 1316
CciI TCATGA 2 cut(s) 313, 912
CfoI GCGC 2 cut(s) 28, 401
Cfr10I RCCGGY 1 cut(s) 1265
Cfr13I GGNCC 2 cut(s) 33, 1657
CseI GACGC 2 cut(s) 32, 1359
CsiI ACCWGGT 1 cut(s) 1653
Csp6I GTAC 3 cut(s) 151, 590, 1151
CviQI GTAC 3 cut(s) 151, 590, 1151
DdeI CTNAG 3 cut(s) 276, 597, 1467
DpnI GATC 3 cut(s) 765, 1065, 1483
DpnII GATC 3 cut(s) 763, 1063, 1481
DraI TTTAAA 1 cut(s) 627
EaeI YGGCCR 1 cut(s) 656
Eam1104I CTCTTC 3 cut(s) 237, 798, 969
EarI CTCTTC 3 cut(s) 237, 798, 969
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 3 cut(s) 108, 1120, 1545
Eco147I AGGCCT 1 cut(s) 387
Eco24I GRGCYC 2 cut(s) 117, 820
Eco47I GGWCC 1 cut(s) 1657
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 261
Eco81I CCTNAGG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 525
EcoICRI GAGCTC 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 390, 1653
EcoT14I CCWWGG 3 cut(s) 108, 1120, 1545
EcoT22I ATGCAT 1 cut(s) 942
EcoT38I GRGCYC 2 cut(s) 117, 820
ErhI CCWWGG 3 cut(s) 108, 1120, 1545
Esp3I CGTCTC 1 cut(s) 968
FalI AAGNNNNNCTT 2 cut(s) 984, 1016
FauI CCCGC 2 cut(s) 606, 899
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 1 cut(s) 792
FokI GGATG 4 cut(s) 316, 421, 674, 722
FriOI GRGCYC 2 cut(s) 117, 820
Fsp4HI GCNGC 1 cut(s) 792
FspBI CTAG 5 cut(s) 383, 417, 1071, 1091, 1616
GlaI GCGC 2 cut(s) 27, 400
GluI GCNGC 1 cut(s) 792
GsuI CTGGAG 3 cut(s) 374, 761, 972
HaeIII GGCC 4 cut(s) 34, 387, 658, 1650
HapII CCGG 3 cut(s) 659, 1266, 1355
HgaI GACGC 2 cut(s) 32, 1359
HhaI GCGC 2 cut(s) 28, 401
Hin6I GCGC 2 cut(s) 26, 399
HinP1I GCGC 2 cut(s) 26, 399
HincII GTYRAC 2 cut(s) 505, 1374
HindII GTYRAC 2 cut(s) 505, 1374
HpaII CCGG 3 cut(s) 659, 1266, 1355
HphI GGTGA 2 cut(s) 64, 1447
Hpy166II GTNNAC 6 cut(s) 505, 539, 824, 1284, 1374, 1540
Hpy188I TCNGA 5 cut(s) 73, 453, 569, 1133, 1330
Hpy188III TCNNGA 8 cut(s) 221, 278, 314, 417, 913, 989, 1071, 1338
Hpy8I GTNNAC 6 cut(s) 505, 539, 824, 1284, 1374, 1540
Hpy99I CGWCG 1 cut(s) 1163
HpyAV CCTTC 3 cut(s) 190, 285, 503
HpyCH4III ACNGT 3 cut(s) 920, 1502, 1533
HpyCH4IV ACGT 2 cut(s) 329, 1260
HpyF10VI GCNNNNNNNGC 4 cut(s) 835, 950, 1271, 1319
HpyF3I CTNAG 3 cut(s) 276, 597, 1467
HpySE526I ACGT 2 cut(s) 329, 1260
HspAI GCGC 2 cut(s) 26, 399
Kzo9I GATC 3 cut(s) 763, 1063, 1481
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 6 cut(s) 112, 120, 197, 230, 742, 835
Lsp1109I GCAGC 1 cut(s) 778
LweI GCATC 5 cut(s) 294, 399, 835, 1172, 1322
MabI ACCWGGT 1 cut(s) 1653
MaeI CTAG 5 cut(s) 383, 417, 1071, 1091, 1616
MaeII ACGT 2 cut(s) 329, 1260
MaeIII GTNAC 1 cut(s) 1382
MalI GATC 3 cut(s) 765, 1065, 1483
MboI GATC 3 cut(s) 763, 1063, 1481
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 2 cut(s) 1063, 1481
MhlI GDGCHC 3 cut(s) 117, 621, 820
MluCI AATT 6 cut(s) 318, 486, 531, 1094, 1144, 1305
MlyI GAGTC 4 cut(s) 14, 304, 815, 979
MmeI TCCRAC 3 cut(s) 312, 1204, 1636
MnlI CCTC 9 cut(s) 398, 592, 921, 1127, 1129, 1455, 1544, 1567, 1580
Mph1103I ATGCAT 1 cut(s) 942
MroXI GAANNNNTTC 1 cut(s) 1440
MseI TTAA 4 cut(s) 626, 1050, 1113, 1407
MspI CCGG 3 cut(s) 659, 1266, 1355
MspR9I CCNGG 2 cut(s) 392, 1655
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 2 cut(s) 401, 1601
MvaI CCWGG 2 cut(s) 392, 1655
MvnI CGCG 3 cut(s) 26, 401, 894
MwoI GCNNNNNNNGC 4 cut(s) 835, 950, 1271, 1319
NcoI CCATGG 2 cut(s) 1120, 1545
NdeII GATC 3 cut(s) 763, 1063, 1481
NlaIV GGNNCC 5 cut(s) 35, 178, 618, 715, 1483
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 3 cut(s) 299, 791, 944
NspV TTCGAA 1 cut(s) 322
PaeI GCATGC 1 cut(s) 944
PagI TCATGA 2 cut(s) 313, 912
PceI AGGCCT 1 cut(s) 387
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PctI GAATGC 2 cut(s) 401, 1601
PdmI GAANNNNTTC 1 cut(s) 1440
PfeI GAWTC 6 cut(s) 564, 697, 1192, 1234, 1331, 1412
PflMI CCANNNNNTGG 1 cut(s) 723
PfoI TCCNGGA 1 cut(s) 390
PkrI GCNGC 1 cut(s) 793
PleI GAGTC 4 cut(s) 13, 304, 814, 979
PpsI GAGTC 4 cut(s) 13, 304, 814, 979
Psp124BI GAGCTC 1 cut(s) 117
Psp1406I AACGTT 1 cut(s) 1260
Psp6I CCWGG 2 cut(s) 390, 1653
PspGI CCWGG 2 cut(s) 390, 1653
PspN4I GGNNCC 5 cut(s) 35, 178, 618, 715, 1483
PspPI GGNCC 2 cut(s) 33, 1657
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 2 cut(s) 1063, 1481
RsaI GTAC 3 cut(s) 152, 591, 1152
RsaNI GTAC 3 cut(s) 151, 590, 1151
SacI GAGCTC 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 4 cut(s) 626, 1050, 1113, 1407
SatI GCNGC 1 cut(s) 792
Sau3AI GATC 3 cut(s) 763, 1063, 1481
Sau96I GGNCC 2 cut(s) 33, 1657
SchI GAGTC 4 cut(s) 14, 304, 815, 979
ScrFI CCNGG 2 cut(s) 392, 1655
SduI GDGCHC 3 cut(s) 117, 621, 820
SexAI ACCWGGT 1 cut(s) 1653
SfaNI GCATC 5 cut(s) 294, 399, 835, 1172, 1322
SfcI CTRYAG 1 cut(s) 434
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 1 cut(s) 1657
SphI GCATGC 1 cut(s) 944
Sse9I AATT 6 cut(s) 318, 486, 531, 1094, 1144, 1305
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 4 cut(s) 138, 572, 613, 892
SspMI CTAG 5 cut(s) 383, 417, 1071, 1091, 1616
SstI GAGCTC 1 cut(s) 117
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 390, 1653
StyI CCWWGG 3 cut(s) 108, 1120, 1545
TaaI ACNGT 3 cut(s) 920, 1502, 1533
TaiI ACGT 2 cut(s) 332, 1263
TaqI TCGA 2 cut(s) 322, 1581
TasI AATT 6 cut(s) 318, 486, 531, 1094, 1144, 1305
TfiI GAWTC 6 cut(s) 564, 697, 1192, 1234, 1331, 1412
Tru1I TTAA 4 cut(s) 626, 1050, 1113, 1407
Tru9I TTAA 4 cut(s) 626, 1050, 1113, 1407
TscAI CASTG 2 cut(s) 561, 1108
TseI GCWGC 1 cut(s) 791
TspGWI ACGGA 4 cut(s) 138, 722, 848, 992
TspRI CASTG 2 cut(s) 561, 1108
Van91I CCANNNNNTGG 1 cut(s) 723
VpaK11BI GGWCC 1 cut(s) 1657
XapI RAATTY 2 cut(s) 318, 1144
XbaI TCTAGA 2 cut(s) 416, 1070
XceI RCATGY 3 cut(s) 299, 791, 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 1440
XspI CTAG 5 cut(s) 383, 417, 1071, 1091, 1616
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.