MD04G1002700.v1.1

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011327
Physical Location & Seq
Forward (+)
402116 .. 409617
7502 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1002700.v1.1.491

Sequence Viewer

Length: 2604 bp
ATGAGTAGGTCCTTCCAACGCCTCTCAATATTTAATATGCCAGCCTCACCACAACCCACATTTTCCGAGTCCACTTCTTCCTTTTCTGCTCTGCTTCCACACACACCCACACACGCACTCTCTCTCTCTCTCAAGTTCTCAACAAAGAAAGAAAGAAAGAAGAAAGCGTCTCAAATCGGCTCCCGCCCGATGGACGCAGCGCCACAGCTGGTCTGCAGCGGAATCGGCATCTCCACCTCCAAACATTCATTTCCAAACACAAATACTAGAATTCGCATTCCCAAACGACTTCACCGTATTTTAGCGGTTGCCACCGACCCCAAACCCACTCGCTCCGGGTCGCCAGGCTCCTCCAATGGCTCCTCACGCACTCCGCCATCGAAATCTCTCAACAGGGTTTCCAACGTCTCGCTGCCCAAATCTCCGCCCCCAAACGCCGTCAACAAACCTATCAATGGAGTTGGAGTTTCCACGAGAATTGGAGATGTTTCAACTGAAATCAAGAGAGTGAGGGCACAGATGGAAGAAAATGAAGACTTGGCAATACTCATGAGAGGTCTTCGGGGACAAAATCTAAGCGACTCACAGTTCGCTCAGGATGACGTTGAGCTTCGCCTTGTTGAGGTAGACGAAAGCAGTGAATTTTTGCCTTTGGTGTATGATCCTGATAGTATCTCTGCTTATTGGGGAAAACGGCCACGAGCCGTTTTGACACGTGTAACCCAATTACTCTCTGTTGCTGGGGGTTTTCTCTCGCGTCTTGTGTGGGATATTATAAACAAGAAGGTCAAGGAGAATGAAGTTGCTAGAGCCATTGAATTAAGGGAAATTGTAACCTCTTTGGGTCCTGCATATATCAAACTTGGTCAAGCTCTGAGCATTCGACCTGATATACTGTCACCTGCTGCAATGACTGAGCTGCAAAAGCTTTGTGATAAGGTTCCTTCATTTCCAGATGATATAGCTATGGCTCTCATTGAGGAGGAGCTTGGTCAGCCATGGCCTAACATCTACTCTGAGCTTTCTCCTTCTCCAATTGCCGCTGCATCTCTCGGGCAAGTATATAAGGGACGGCTGAAAGAAAATGGAGATCTCGTGGCTGTTAAAGTGCAACGGCCTTTTGTTCTTGAAACAGTGACTGTTGATTTGTTTGTCATACGCAACTTGGGTTTGGTTCTCCGGAAGTTTCCTCAGATCTCGATAGATGTGGTTGGATTAGTTGATGAATGGGCAGCTCGTTTTTTTGAAGAGCTGGATTATGTTAATGAGGGTGAAAATGGAACAATCTTTGCTGATTTGATGAGGAAGGACCTTCCACAGGTAGTTGTGCCAAAAACCTACCAGAAATATACTTCAAGAAAGGTTCTTACTACAGGATGGATAGATGGAGAAAAGTTATCTCAGAGTACTGAAAGTGATGTTGGAGAACTGGTTAATGTTGGAGTCATATGTTACTTGAAGCAGTTGCTTGATACTGGAATGTTTCATGCTGATCCTCATCCTGGGAATATGATTCGCACTCCAGATGGGAAGCTTGCCATACTTGACTTTGGTCTTGTCACTAAATTAACTGATGATCAGAAGTATGGAATGATCGAGGCAATTGCTCACCTTATCCATCGTGACTATGCTGCCATAGTTAAAGACTTTGTCAAGCTTGAATTCATTTCAGAGGGGGTTAATCTAGAACCTATTTTGCCTGTTCTGGCCAAGGTTTTTGATCAGGCTCTTGAAGGTGGAGGAGCAAAAAATATCAACTTCCAGGAGTTGGCATCAGATTTGGCTCAAATCACATTCGACTATCCATTTAGGATACCCCCTTATTTTGCTCTTATAATTAGGGCAATTGGGGTCCTGGAAGGCATAGCTTTAGTAGGAAATCCTGATTTTGCCATTGTGGATGAGGCCTATCCGTATATTGCCCAGAGGCTCCTGACTGATGAATCCCCACGGCTAAGGGGTGCATTGCGTTATACAATATATGGGAAATCTGGAGTGTTTGATGCTGAGAGATTTATTGATGTGATGCAAGCCTTTGAAACTTTCATAACTGCAGCTAAAAGTGGAGGTGGAGAGGAACTGAATGGTGAAATGGCTGAACTTGGTATTCTGCAAGGTCAAACCGAGTCACCCTTTCCCGGATTTATGTCAAATGGCCAACCAATACAAACAAGGACGGCTTTAGCATTTTTACTGTCTGATAAAGGGAGCTTTTTCAGAGAATTTCTTCTGGATGAGATTGTCAAAGGCATTGATGCAGTTACGAGGGAACAATTGGTTCGTACAATGGCTATTCTTGGATTGGGGAATCCTACCCCAGTTTTCAGCATGGTTCCTACCTTCGGGTTGTTCAAGCCAGCAGGACTTGTACCAACAATAACCGAAGAGGATAGAGTTATATTGAACAACGTCCAAACAATACTCGAGTTCCTGACTGCAGGAAGTTCCATGTCAAGGATATCAAATCAGGGTTTGAATGTGTCCCAGGTTATCCAAGAGCTTCTTCCCGTTTTGCCGAGCATCTCTTCCAAAGTGCTCCCTGAAGTGCTCAGTCGATTGTCTTCACGGGTAATAGCACGCGTGATTCGAGATACAATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 APH

Protein Analysis

868

Amino Acids

95.35

Weight (kDa)

6.09

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 310 - 548 1.1e-62 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 776, 1835
AarI CACCTGC 1 cut(s) 910
Acc36I ACCTGC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 1768
AccI GTMKAC 1 cut(s) 627
AccII CGCG 2 cut(s) 757, 2580
AccIII TCCGGA 1 cut(s) 1179
AciI CCGC 6 cut(s) 184, 219, 305, 374, 425, 1041
AclWI GGATC 2 cut(s) 656, 1487
AcoI YGGCCR 3 cut(s) 695, 1707, 2155
AcsI RAATTY 4 cut(s) 270, 641, 1661, 2222
AcuI CTGAAG 1 cut(s) 2562
AcvI CACGTG 1 cut(s) 716
AfaI GTAC 3 cut(s) 1408, 2284, 2370
AfiI CCNNNNNNNGG 9 cut(s) 190, 455, 622, 1318, 1502, 1768, 2138, 2342, 2454
AflIII ACRYGT 3 cut(s) 713, 715, 2578
AjnI CCWGG 5 cut(s) 343, 1501, 1761, 1854, 2484
AjuI GAANNNNNNNTTGG 4 cut(s) 1404, 1436, 2487, 2519
Alw21I GWGCWC 2 cut(s) 2538, 2550
Alw26I GTCTC 2 cut(s) 174, 412
AlwI GGATC 2 cut(s) 656, 1487
AlwNI CAGNNNCTG 1 cut(s) 1139
Ama87I CYCGRG 2 cut(s) 1052, 2423
Aor13HI TCCGGA 1 cut(s) 1179
AoxI GGCC 6 cut(s) 695, 1001, 1115, 1707, 1905, 2155
ApeKI GCWGC 9 cut(s) 197, 216, 412, 905, 919, 1043, 1232, 1631, 2054
ApoI RAATTY 4 cut(s) 270, 641, 1661, 2222
ArsI GACNNNNNNTTYG 4 cut(s) 572, 604, 1129, 1161
Asp700I GAANNNNTTC 1 cut(s) 2226
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 4 cut(s) 9, 845, 1309, 1852
AsuC2I CCSGG 2 cut(s) 337, 2139
AsuHPI GGTGA 7 cut(s) 39, 284, 891, 1283, 1601, 2099, 2121
AvaI CYCGRG 2 cut(s) 1052, 2423
AvaII GGWCC 4 cut(s) 9, 845, 1309, 1852
BaeGI GKGCMC 1 cut(s) 517
BalI TGGCCA 2 cut(s) 1709, 2157
BauI CACGAG 3 cut(s) 472, 699, 1094
BbrPI CACGTG 1 cut(s) 716
BbsI GAAGAC 3 cut(s) 540, 551, 2553
Bbv12I GWGCWC 2 cut(s) 2538, 2550
BbvI GCAGC 9 cut(s) 209, 228, 399, 892, 906, 1030, 1244, 1618, 2066
BccI CCATC 7 cut(s) 184, 385, 514, 1371, 1379, 1520, 1626
BceAI ACGGC 7 cut(s) 422, 689, 710, 1088, 1130, 1967, 2193
BcgI CGANNNNNNTGC 4 cut(s) 205, 239, 1586, 1620
BciT130I CCWGG 5 cut(s) 345, 1503, 1763, 1856, 2486
BciVI GTATCC 1 cut(s) 1806
BclI TGATCA 2 cut(s) 1576, 1720
BcnI CCSGG 2 cut(s) 337, 2139
BcoDI GTCTC 2 cut(s) 174, 412
BfaI CTAG 3 cut(s) 267, 807, 1685
BfmI CTRYAG 4 cut(s) 214, 1371, 2052, 2436
BfoI RGCGCY 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 910
BfuI GTATCC 1 cut(s) 1806
BglII AGATCT 2 cut(s) 1090, 1194
BmcAI AGTACT 1 cut(s) 1408
Bme1390I CCNGG 7 cut(s) 337, 345, 1503, 1763, 1856, 2139, 2486
Bme18I GGWCC 4 cut(s) 9, 845, 1309, 1852
BmeT110I CYCGRG 2 cut(s) 1052, 2423
BmgT120I GGNCC 4 cut(s) 9, 845, 1309, 1852
BmiI GGNNCC 8 cut(s) 181, 349, 361, 846, 942, 1853, 1931, 2334
BmrFI CCNGG 7 cut(s) 337, 345, 1503, 1763, 1856, 2139, 2486
BmrI ACTGGG 1 cut(s) 2312
BmsI GCATC 7 cut(s) 237, 1055, 1781, 1993, 2016, 2245, 2529
BmuI ACTGGG 1 cut(s) 2312
BoxI GACNNNNGTC 1 cut(s) 1551
BpiI GAAGAC 3 cut(s) 540, 551, 2553
BpmI CTGGAG 2 cut(s) 1506, 2013
Bpu10I CCTNAGC 2 cut(s) 594, 1955
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 2 cut(s) 337, 2139
BsaAI YACGTR 1 cut(s) 716
BsaBI GATNNNNATC 1 cut(s) 1497
BsaJI CCNNGG 5 cut(s) 998, 1502, 1710, 1949, 2484
BsaWI WCCGGW 1 cut(s) 1179
BsaXI ACNNNNNCTCC 6 cut(s) 450, 480, 1080, 1110, 1416, 1446
Bsc4I CCNNNNNNNGG 9 cut(s) 190, 455, 622, 1318, 1502, 1768, 2138, 2342, 2454
Bse1I ACTGG 3 cut(s) 1434, 1480, 2318
Bse3DI GCAATG 2 cut(s) 915, 1964
Bse8I GATNNNNATC 1 cut(s) 1497
BseAI TCCGGA 1 cut(s) 1179
BseBI CCWGG 5 cut(s) 345, 1503, 1763, 1856, 2486
BseDI CCNNGG 5 cut(s) 998, 1502, 1710, 1949, 2484
BseGI GGATG 5 cut(s) 604, 1382, 1498, 1906, 2239
BseJI GATNNNNATC 1 cut(s) 1497
BseLI CCNNNNNNNGG 9 cut(s) 190, 455, 622, 1318, 1502, 1768, 2138, 2342, 2454
BseMI GCAATG 2 cut(s) 915, 1964
BseMII CTCAG 8 cut(s) 608, 866, 906, 1008, 1205, 1415, 1998, 2563
BseNI ACTGG 3 cut(s) 1434, 1480, 2318
BseRI GAGGAG 5 cut(s) 340, 352, 995, 998, 1755
BseSI GKGCMC 1 cut(s) 517
BseXI GCAGC 9 cut(s) 209, 228, 399, 892, 906, 1030, 1244, 1618, 2066
BseYI CCCAGC 1 cut(s) 740
Bsh1236I CGCG 2 cut(s) 757, 2580
BshFI GGCC 6 cut(s) 697, 1003, 1117, 1709, 1907, 2157
BsiHKAI GWGCWC 2 cut(s) 2538, 2550
BsiHKCI CYCGRG 2 cut(s) 1052, 2423
BsiSI CCGG 3 cut(s) 336, 1180, 2139
BslFI GGGAC 3 cut(s) 579, 1083, 2467
BslI CCNNNNNNNGG 9 cut(s) 190, 455, 622, 1318, 1502, 1768, 2138, 2342, 2454
BsmAI GTCTC 2 cut(s) 174, 412
BsmBI CGTCTC 2 cut(s) 174, 412
BsmFI GGGAC 3 cut(s) 579, 1083, 2467
BsmI GAATGC 2 cut(s) 276, 879
BsnI GGCC 6 cut(s) 697, 1003, 1117, 1709, 1907, 2157
BsoBI CYCGRG 2 cut(s) 1052, 2423
Bsp1286I GDGCHC 3 cut(s) 517, 2538, 2550
Bsp13I TCCGGA 1 cut(s) 1179
Bsp143I GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
Bsp19I CCATGG 1 cut(s) 998
BspACI CCGC 6 cut(s) 184, 219, 305, 374, 425, 1041
BspANI GGCC 6 cut(s) 697, 1003, 1117, 1709, 1907, 2157
BspCNI CTCAG 8 cut(s) 607, 867, 907, 1009, 1204, 1414, 1999, 2562
BspEI TCCGGA 1 cut(s) 1179
BspFNI CGCG 2 cut(s) 757, 2580
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 8 cut(s) 181, 349, 361, 846, 942, 1853, 1931, 2334
BspMAI CTGCAG 3 cut(s) 218, 2056, 2440
BspMI ACCTGC 1 cut(s) 910
BspPI GGATC 2 cut(s) 656, 1487
BspQI GCTCTTC 1 cut(s) 1242
BsrDI GCAATG 2 cut(s) 915, 1964
BsrI ACTGG 3 cut(s) 1434, 1480, 2318
BssECI CCNNGG 5 cut(s) 998, 1502, 1710, 1949, 2484
BssMI GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
BssSI CACGAG 3 cut(s) 472, 699, 1094
BssT1I CCWWGG 2 cut(s) 998, 1710
Bst2BI CACGAG 3 cut(s) 472, 699, 1094
Bst2UI CCWGG 5 cut(s) 345, 1503, 1763, 1856, 2486
Bst4CI ACNGT 6 cut(s) 296, 588, 897, 1135, 1141, 2196
Bst6I CTCTTC 3 cut(s) 1242, 2379, 2530
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 5 cut(s) 42, 1536, 2031, 2358, 2578
BstDSI CCRYGG 2 cut(s) 998, 1949
BstENI CCTNNNNNAGG 2 cut(s) 620, 1316
BstF5I GGATG 5 cut(s) 604, 1382, 1498, 1906, 2239
BstFNI CGCG 2 cut(s) 757, 2580
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 7 cut(s) 664, 1093, 1197, 1495, 1579, 1596, 1723
BstMAI GTCTC 2 cut(s) 174, 412
BstMBI GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
BstMWI GCNNNNNNNGC 4 cut(s) 225, 366, 925, 994
BstNI CCWGG 5 cut(s) 345, 1503, 1763, 1856, 2486
BstPAI GACNNNNGTC 1 cut(s) 1551
BstSCI CCNGG 7 cut(s) 335, 343, 1501, 1761, 1854, 2137, 2484
BstSFI CTRYAG 4 cut(s) 214, 1371, 2052, 2436
BstSLI GKGCMC 1 cut(s) 517
BstUI CGCG 2 cut(s) 757, 2580
BstV1I GCAGC 9 cut(s) 209, 228, 399, 892, 906, 1030, 1244, 1618, 2066
BstV2I GAAGAC 3 cut(s) 540, 551, 2553
BstX2I RGATCY 2 cut(s) 1090, 1194
BstYI RGATCY 2 cut(s) 1090, 1194
BsuI GTATCC 1 cut(s) 1806
BsuRI GGCC 6 cut(s) 697, 1003, 1117, 1709, 1907, 2157
BtgI CCRYGG 2 cut(s) 998, 1949
BtsCI GGATG 5 cut(s) 604, 1382, 1498, 1906, 2239
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 2 cut(s) 643, 1140
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 5 cut(s) 42, 1536, 2031, 2358, 2578
CaiI CAGNNNCTG 1 cut(s) 1139
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 4 cut(s) 9, 845, 1309, 1852
CseI GACGC 3 cut(s) 156, 203, 746
Csp6I GTAC 3 cut(s) 1407, 2283, 2369
CspCI CAANNNNNGTGG 2 cut(s) 460, 495
CviAII CATG 5 cut(s) 550, 999, 1487, 2329, 2449
CviQI GTAC 3 cut(s) 1407, 2283, 2369
DpnI GATC 7 cut(s) 663, 1092, 1196, 1494, 1578, 1595, 1722
DpnII GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
EaeI YGGCCR 3 cut(s) 695, 1707, 2155
Eam1104I CTCTTC 3 cut(s) 1242, 2379, 2530
EarI CTCTTC 3 cut(s) 1242, 2379, 2530
EciI GGCGGA 2 cut(s) 363, 414
Eco130I CCWWGG 2 cut(s) 998, 1710
Eco147I AGGCCT 1 cut(s) 1907
Eco32I GATATC 1 cut(s) 2460
Eco47I GGWCC 4 cut(s) 9, 845, 1309, 1852
Eco57I CTGAAG 1 cut(s) 2562
Eco72I CACGTG 1 cut(s) 716
Eco88I CYCGRG 2 cut(s) 1052, 2423
EcoNI CCTNNNNNAGG 2 cut(s) 620, 1316
EcoO109I RGGNCCY 4 cut(s) 9, 845, 1309, 1852
EcoRI GAATTC 2 cut(s) 270, 1661
EcoRII CCWGG 5 cut(s) 343, 1501, 1761, 1854, 2484
EcoRV GATATC 1 cut(s) 2460
EcoT14I CCWWGG 2 cut(s) 998, 1710
ErhI CCWWGG 2 cut(s) 998, 1710
Esp3I CGTCTC 2 cut(s) 174, 412
FaeI CATG 5 cut(s) 553, 1002, 1490, 2332, 2452
FalI AAGNNNNNCTT 4 cut(s) 2164, 2196, 2487, 2519
FaqI GGGAC 3 cut(s) 579, 1083, 2467
FatI CATG 5 cut(s) 549, 998, 1486, 2328, 2448
FauI CCCGC 1 cut(s) 191
FauNDI CATATG 1 cut(s) 1448
FbaI TGATCA 2 cut(s) 1576, 1720
FblI GTMKAC 1 cut(s) 627
FokI GGATG 5 cut(s) 611, 1389, 1485, 1913, 2246
FspBI CTAG 3 cut(s) 267, 807, 1685
GlaI GCGC 1 cut(s) 201
GsaI CCCAGC 1 cut(s) 744
GsuI CTGGAG 2 cut(s) 1506, 2013
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 6 cut(s) 697, 1003, 1117, 1709, 1907, 2157
HapII CCGG 3 cut(s) 336, 1180, 2139
HgaI GACGC 3 cut(s) 156, 203, 746
HhaI GCGC 1 cut(s) 202
Hin1II CATG 5 cut(s) 553, 1002, 1490, 2332, 2452
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 3 cut(s) 926, 1532, 1655
HinfI GANTC 9 cut(s) 68, 222, 581, 1443, 1513, 1943, 2126, 2308, 2584
HpaII CCGG 3 cut(s) 336, 1180, 2139
HphI GGTGA 7 cut(s) 39, 284, 891, 1283, 1601, 2099, 2121
Hpy166II GTNNAC 3 cut(s) 72, 442, 628
Hpy8I GTNNAC 3 cut(s) 72, 442, 628
HpyAV CCTTC 9 cut(s) 22, 778, 954, 1038, 1300, 1322, 1727, 1853, 2350
HpyCH4III ACNGT 6 cut(s) 296, 588, 897, 1135, 1141, 2196
HpyCH4IV ACGT 4 cut(s) 405, 603, 715, 2409
HpyF10VI GCNNNNNNNGC 4 cut(s) 225, 366, 925, 994
HpySE526I ACGT 4 cut(s) 405, 603, 715, 2409
Hsp92II CATG 5 cut(s) 553, 1002, 1490, 2332, 2452
HspAI GCGC 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 1179
Ksp22I TGATCA 2 cut(s) 1576, 1720
Kzo9I GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
LguI GCTCTTC 1 cut(s) 1242
LmnI GCTCC 9 cut(s) 185, 338, 353, 365, 985, 1742, 1935, 2208, 2541
Lsp1109I GCAGC 9 cut(s) 209, 228, 399, 892, 906, 1030, 1244, 1618, 2066
LweI GCATC 7 cut(s) 237, 1055, 1781, 1993, 2016, 2245, 2529
MaeI CTAG 3 cut(s) 267, 807, 1685
MaeII ACGT 4 cut(s) 405, 603, 715, 2409
MaeIII GTNAC 9 cut(s) 718, 832, 897, 1135, 1451, 1558, 1622, 2127, 2260
MalI GATC 7 cut(s) 663, 1092, 1196, 1494, 1578, 1595, 1722
MboI GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
MfeI CAATTG 4 cut(s) 1035, 1602, 1845, 2273
MflI RGATCY 2 cut(s) 1090, 1194
MhlI GDGCHC 3 cut(s) 517, 2538, 2550
MlsI TGGCCA 2 cut(s) 1709, 2157
MluI ACGCGT 1 cut(s) 2578
MluNI TGGCCA 2 cut(s) 1709, 2157
MlyI GAGTC 4 cut(s) 77, 575, 1452, 2135
MmeI TCCRAC 6 cut(s) 40, 426, 442, 1192, 1402, 1420
Mox20I TGGCCA 2 cut(s) 1709, 2157
MroI TCCGGA 1 cut(s) 1179
MroXI GAANNNNTTC 1 cut(s) 2226
MscI TGGCCA 2 cut(s) 1709, 2157
MseI TTAA 8 cut(s) 33, 821, 1104, 1263, 1434, 1568, 1641, 1680
Msp20I TGGCCA 2 cut(s) 1709, 2157
MspA1I CMGCKG 3 cut(s) 208, 219, 1043
MspI CCGG 3 cut(s) 336, 1180, 2139
MspR9I CCNGG 7 cut(s) 337, 345, 1503, 1763, 1856, 2139, 2486
MunI CAATTG 4 cut(s) 1035, 1602, 1845, 2273
Mva1269I GAATGC 2 cut(s) 276, 879
MvaI CCWGG 5 cut(s) 345, 1503, 1763, 1856, 2486
MvnI CGCG 2 cut(s) 757, 2580
MwoI GCNNNNNNNGC 4 cut(s) 225, 366, 925, 994
NciI CCSGG 2 cut(s) 337, 2139
NcoI CCATGG 1 cut(s) 998
NdeI CATATG 1 cut(s) 1448
NdeII GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
NlaIII CATG 5 cut(s) 553, 1002, 1490, 2332, 2452
NlaIV GGNNCC 8 cut(s) 181, 349, 361, 846, 942, 1853, 1931, 2334
NmeAIII GCCGAG 1 cut(s) 2541
NmuCI GTSAC 5 cut(s) 897, 1135, 1558, 1622, 2127
PaeR7I CTCGAG 1 cut(s) 2423
PagI TCATGA 1 cut(s) 549
PaqCI CACCTGC 1 cut(s) 910
PceI AGGCCT 1 cut(s) 1907
PciSI GCTCTTC 1 cut(s) 1242
PcsI WCGNNNNNNNCGW 2 cut(s) 292, 2584
PctI GAATGC 2 cut(s) 276, 879
PdmI GAANNNNTTC 1 cut(s) 2226
PfeI GAWTC 5 cut(s) 222, 1513, 1943, 2308, 2584
PflFI GACNNNGTC 1 cut(s) 1649
PflMI CCANNNNNTGG 1 cut(s) 1768
PfoI TCCNGGA 3 cut(s) 1761, 1854, 2137
PleI GAGTC 4 cut(s) 76, 575, 1451, 2134
PmaCI CACGTG 1 cut(s) 716
PmlI CACGTG 1 cut(s) 716
PpsI GAGTC 4 cut(s) 76, 575, 1451, 2134
Ppu21I YACGTR 1 cut(s) 716
PpuMI RGGWCCY 4 cut(s) 9, 845, 1309, 1852
PshAI GACNNNNGTC 1 cut(s) 1551
PsiI TTATAA 2 cut(s) 776, 1835
Psp5II RGGWCCY 4 cut(s) 9, 845, 1309, 1852
Psp6I CCWGG 5 cut(s) 343, 1501, 1761, 1854, 2484
PspCI CACGTG 1 cut(s) 716
PspFI CCCAGC 1 cut(s) 740
PspGI CCWGG 5 cut(s) 343, 1501, 1761, 1854, 2484
PspN4I GGNNCC 8 cut(s) 181, 349, 361, 846, 942, 1853, 1931, 2334
PspPI GGNCC 4 cut(s) 9, 845, 1309, 1852
PspPPI RGGWCCY 4 cut(s) 9, 845, 1309, 1852
PspXI VCTCGAGB 1 cut(s) 2423
PstI CTGCAG 3 cut(s) 218, 2056, 2440
PstNI CAGNNNCTG 1 cut(s) 1139
PsuI RGATCY 2 cut(s) 1090, 1194
PsyI GACNNNGTC 1 cut(s) 1649
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 3 cut(s) 1408, 2284, 2370
RsaNI GTAC 3 cut(s) 1407, 2283, 2369
SapI GCTCTTC 1 cut(s) 1242
SaqAI TTAA 8 cut(s) 33, 821, 1104, 1263, 1434, 1568, 1641, 1680
Sau3AI GATC 7 cut(s) 661, 1090, 1194, 1492, 1576, 1593, 1720
Sau96I GGNCC 4 cut(s) 9, 845, 1309, 1852
ScaI AGTACT 1 cut(s) 1408
SchI GAGTC 4 cut(s) 77, 575, 1452, 2135
ScrFI CCNGG 7 cut(s) 337, 345, 1503, 1763, 1856, 2139, 2486
SduI GDGCHC 3 cut(s) 517, 2538, 2550
SfaNI GCATC 7 cut(s) 237, 1055, 1781, 1993, 2016, 2245, 2529
SfcI CTRYAG 4 cut(s) 214, 1371, 2052, 2436
Sfr274I CTCGAG 1 cut(s) 2423
SinI GGWCC 4 cut(s) 9, 845, 1309, 1852
SlaI CTCGAG 1 cut(s) 2423
SmlI CTYRAG 2 cut(s) 131, 2423
SmoI CTYRAG 2 cut(s) 131, 2423
SseBI AGGCCT 1 cut(s) 1907
SsiI CCGC 6 cut(s) 184, 219, 305, 374, 425, 1041
SspI AATATT 1 cut(s) 30
SspMI CTAG 3 cut(s) 267, 807, 1685
StuI AGGCCT 1 cut(s) 1907
StyD4I CCNGG 7 cut(s) 335, 343, 1501, 1761, 1854, 2137, 2484
StyI CCWWGG 2 cut(s) 998, 1710
TaaI ACNGT 6 cut(s) 296, 588, 897, 1135, 1141, 2196
TaiI ACGT 4 cut(s) 408, 606, 718, 2412
TaqI TCGA 8 cut(s) 380, 883, 1199, 1596, 1797, 2424, 2554, 2587
TatI WGTACW 1 cut(s) 1406
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 5 cut(s) 222, 1513, 1943, 2308, 2584
Tru1I TTAA 8 cut(s) 33, 821, 1104, 1263, 1434, 1568, 1641, 1680
Tru9I TTAA 8 cut(s) 33, 821, 1104, 1263, 1434, 1568, 1641, 1680
TscAI CASTG 2 cut(s) 643, 1140
TseFI GTSAC 5 cut(s) 897, 1135, 1558, 1622, 2127
TseI GCWGC 9 cut(s) 197, 216, 412, 905, 919, 1043, 1232, 1631, 2054
Tsp45I GTSAC 5 cut(s) 897, 1135, 1558, 1622, 2127
TspDTI ATGAA 9 cut(s) 237, 546, 813, 936, 1239, 1475, 1654, 1956, 2035
TspGWI ACGGA 1 cut(s) 1902
TspRI CASTG 2 cut(s) 643, 1140
Tth111I GACNNNGTC 1 cut(s) 1649
Van91I CCANNNNNTGG 1 cut(s) 1768
VpaK11BI GGWCC 4 cut(s) 9, 845, 1309, 1852
XagI CCTNNNNNAGG 2 cut(s) 620, 1316
XapI RAATTY 4 cut(s) 270, 641, 1661, 2222
XbaI TCTAGA 1 cut(s) 1684
XhoI CTCGAG 1 cut(s) 2423
XmiI GTMKAC 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 2226
XspI CTAG 3 cut(s) 267, 807, 1685
ZrmI AGTACT 1 cut(s) 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.