Rw4G000660

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011327
Physical Location & Seq
Reverse (-)
1279119 .. 1287520
8402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000660.1

Sequence Viewer

Length: 1731 bp
ATGGAGGCAGCGCCTCAGCTCGTCTCCACTGGAATCGAATCCCTTCGCCGGAGCTCCAAGCATTCATTCCCGACCACAACCTCCAGTAGAGTTCGAGTTCGCGTTCCGAACCGATTGAACCGGGTTCTTGCGGTTGCCACCGACCCCAAACCCACCAAGTCCGGCTCATCACCATCAAAGCCACCCAAATCTCCATCGCCAGACTCTGTCAATGGCTCCTCCTCGAGGTCCCCGCCCATCAATGGAGTTTCCACGAGAATTGGTGATGTTTCAAAGGAAATCAAAAGGGTGCGAGCACAGATGGAAGAAAACGAAGACTTGGCAATACTCATGAAAGGTCTTCGCGGACAAAATTTGAAGGATTCACAGTTTGCTGAGGATGATGTTCAGCTTCGTCTTGTTGAGGTAGATGAAAGCAGTGAATTTTTGCCTGTGGTATATGATCCTGCTAGCATCTCTGCTTATTGGGGAAAACGGCCTCGTGCTGTTGTGACACGTGTAATTCAATTACTCTCTGTAACTGGGGGTTTCCTTTCACGTCTTGCAGGGGATTTTATAAACAAGAAAATCAAAGAGAATGAAGTTGCTAGGGCCATTGCAATAAGGGAAATTGTGACCTCTTTGGGTCCCGCATATATCAAACTTGGGCAAGCATTGAGCATTCGACCAGATATACTGTCACCCGCTGCAATGACTGAGCTGCAAAAGCTTTGTGATAAGGTTCCTTCATTTCCGGATGATATAGCTATGGCTCTCATTGAGGAGGAGCTTGGTCAGCCATGGCAAAACATGTACTCTGAGCTCTCTTCCTCTCCAATTGCTGCTGCATCTCTTGGGCAAGTATATAAGGGCCGGCTAAAAGAAAATGGAGATCTCGTGGCTGTTAAAGTGCAGAGACCTTTTGTTCTTGAAACAGTAACTGTTGATTTGTTTATCATACGGAACTTGGGTTTGGTTCTCCGGAGGTTTCCTCAGATCTCTGTAGATGTGGTTGGATTAGTTGATGAATGGGCAGCTCGTTTCTTTGAAGAGTTGGATTATATTAACGAGGGTGAGAATGGGCTACTCTTTGCTGAAATGATGAGAAAAGATCTTCCACAGGTGGTTGTACCAAAAACCTACCCAAAATATACATCAAGAAAGGTTCTTACTACATCATGGTTAGAAGGAGAGAAGCTATCACAAAGTACTGAAAGTGATGTTGGAGAACTGGTCAATGTTGGAGTCATATGCTACCTGAAGCAGTTGCTTGACACTGGGTTATTTCATGCTGATCCTCATCCTGGGAATTTGATTCGCACTCCAGATGGCAAGCTTGCCATACTTGATTTTGGTCTGGTCACCAAGTTAACTGATGATCAAAAGTATGGAATGATTGAGGCAATTGCTCACCTTATTCATCGTGATTATGGAGCCATAGTCAAAGACTTTGTCAAGCTCGGTTTTATTGATGAGGGGGTTAATCTAGAACCTATTTTGCCTGTTCTGGCCAAGGTTTTTGATCAGGCTCTTGAAGGTGGAGGAGCAAAAAATATCAACTTCCAAGACTTGGCAGCAGATTTGGCACAAATCACATTTGACTATCCATTTAAGATACCGCCTTATTTCGCTCTTATAATTAGAGCAATTGGGGTCCTGGAAGGCATAGCTTTAGTGGGAAATCCTGATTTTGCTATTGTGGATGAGGCCTATCCATATATTGCCCAGGTATTTGTACTCTCATTAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 APH

Protein Analysis

576

Amino Acids

63.37

Weight (kDa)

5.49

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 236 - 475 2.3e-63 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 557, 1616
AbsI CCTCGAGG 1 cut(s) 223
AccB7I CCANNNNNTGG 1 cut(s) 1549
AccII CGCG 2 cut(s) 102, 345
AccIII TCCGGA 2 cut(s) 733, 960
AciI CCGC 6 cut(s) 131, 233, 345, 630, 684, 1598
AclWI GGATC 2 cut(s) 437, 1268
AcoI YGGCCR 1 cut(s) 1488
AcsI RAATTY 3 cut(s) 352, 422, 1288
AcuI CTGAAG 1 cut(s) 1259
AcvI CACGTG 1 cut(s) 497
AfaI GTAC 4 cut(s) 794, 1110, 1189, 1716
AfiI CCNNNNNNNGG 5 cut(s) 48, 225, 242, 1283, 1549
AflIII ACRYGT 3 cut(s) 494, 496, 789
AgsI TTSAA 7 cut(s) 118, 273, 358, 506, 911, 1028, 1514
AjiI CACGTC 1 cut(s) 539
AjnI CCWGG 3 cut(s) 1282, 1635, 1704
AjuI GAANNNNNNNTTGG 6 cut(s) 50, 82, 935, 967, 1185, 1217
Alw21I GWGCWC 3 cut(s) 56, 298, 804
Alw26I GTCTC 2 cut(s) 28, 889
AlwI GGATC 2 cut(s) 437, 1268
AlwNI CAGNNNCTG 2 cut(s) 206, 920
Ama87I CYCGRG 1 cut(s) 223
Aor13HI TCCGGA 2 cut(s) 733, 960
AoxI GGCC 5 cut(s) 476, 591, 850, 1488, 1686
ApeKI GCWGC 7 cut(s) 8, 686, 700, 821, 824, 1013, 1553
ApoI RAATTY 3 cut(s) 352, 422, 1288
ArsI GACNNNNNNTTYG 2 cut(s) 1416, 1448
AseI ATTAAT 1 cut(s) 1724
Asp700I GAANNNNTTC 1 cut(s) 42
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 5 cut(s) 228, 591, 626, 850, 1633
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 6 cut(s) 162, 275, 672, 1064, 1333, 1382
AsuNHI GCTAGC 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 3 cut(s) 228, 626, 1633
BalI TGGCCA 1 cut(s) 1490
BanII GRGCYC 2 cut(s) 56, 804
BauI CACGAG 3 cut(s) 253, 480, 875
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 2 cut(s) 321, 332
Bbv12I GWGCWC 3 cut(s) 56, 298, 804
BbvCI CCTCAGC 2 cut(s) 15, 375
BbvI GCAGC 7 cut(s) 20, 673, 687, 808, 811, 1025, 1565
BccI CCATC 5 cut(s) 181, 202, 245, 295, 1301
BceAI ACGGC 1 cut(s) 491
BciT130I CCWGG 3 cut(s) 1284, 1637, 1706
BclI TGATCA 2 cut(s) 1357, 1501
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 2 cut(s) 28, 889
BfaI CTAG 3 cut(s) 450, 588, 1466
BfmI CTRYAG 1 cut(s) 981
BfoI RGCGCY 1 cut(s) 14
BglII AGATCT 3 cut(s) 871, 975, 1090
BisI GCNGC 7 cut(s) 9, 687, 701, 822, 825, 1014, 1554
BlsI GCNGC 7 cut(s) 10, 688, 702, 823, 826, 1015, 1555
BmcAI AGTACT 1 cut(s) 1189
Bme1390I CCNGG 4 cut(s) 122, 1284, 1637, 1706
Bme18I GGWCC 3 cut(s) 228, 626, 1633
BmeT110I CYCGRG 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 539
BmgT120I GGNCC 5 cut(s) 228, 591, 626, 850, 1633
BmiI GGNNCC 7 cut(s) 217, 230, 627, 628, 723, 1414, 1634
BmrFI CCNGG 4 cut(s) 122, 1284, 1637, 1706
BmrI ACTGGG 2 cut(s) 531, 1266
BmsI GCATC 2 cut(s) 462, 836
BmtI GCTAGC 1 cut(s) 453
BmuI ACTGGG 2 cut(s) 531, 1266
BpiI GAAGAC 2 cut(s) 321, 332
BplI GAGNNNNNCTC 2 cut(s) 955, 987
BpmI CTGGAG 2 cut(s) 67, 1287
Bpu10I CCTNAGC 2 cut(s) 15, 375
BpuMI CCSGG 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 1278
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 4 cut(s) 779, 1283, 1491, 1704
BsaWI WCCGGW 2 cut(s) 733, 960
BsaXI ACNNNNNCTCC 6 cut(s) 861, 891, 1197, 1227, 1404, 1434
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 225, 242, 1283, 1549
Bse118I RCCGGY 1 cut(s) 852
Bse1I ACTGG 5 cut(s) 34, 84, 526, 1215, 1261
Bse3DI GCAATG 2 cut(s) 594, 696
Bse8I GATNNNNATC 1 cut(s) 1278
BseAI TCCGGA 2 cut(s) 733, 960
BseBI CCWGG 3 cut(s) 1284, 1637, 1706
BseDI CCNNGG 4 cut(s) 779, 1283, 1491, 1704
BseGI GGATG 4 cut(s) 385, 742, 1279, 1687
BseJI GATNNNNATC 1 cut(s) 1278
BseLI CCNNNNNNNGG 5 cut(s) 48, 225, 242, 1283, 1549
BseMI GCAATG 2 cut(s) 594, 696
BseMII CTCAG 5 cut(s) 29, 366, 687, 789, 986
BseNI ACTGG 5 cut(s) 34, 84, 526, 1215, 1261
BseRI GAGGAG 5 cut(s) 208, 211, 776, 779, 1536
BseXI GCAGC 7 cut(s) 20, 673, 687, 808, 811, 1025, 1565
BsgI GTGCAG 1 cut(s) 911
Bsh1236I CGCG 2 cut(s) 102, 345
BshFI GGCC 5 cut(s) 478, 593, 852, 1490, 1688
BsiHKAI GWGCWC 3 cut(s) 56, 298, 804
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
BslFI GGGAC 2 cut(s) 214, 612
BslI CCNNNNNNNGG 5 cut(s) 48, 225, 242, 1283, 1549
BsmAI GTCTC 2 cut(s) 28, 889
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 2 cut(s) 214, 612
BsmI GAATGC 2 cut(s) 61, 660
BsnI GGCC 5 cut(s) 478, 593, 852, 1490, 1688
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 3 cut(s) 56, 298, 804
Bsp13I TCCGGA 2 cut(s) 733, 960
Bsp143I GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
Bsp19I CCATGG 1 cut(s) 779
BspACI CCGC 6 cut(s) 131, 233, 345, 630, 684, 1598
BspANI GGCC 5 cut(s) 478, 593, 852, 1490, 1688
BspCNI CTCAG 5 cut(s) 28, 367, 688, 790, 985
BspEI TCCGGA 2 cut(s) 733, 960
BspFNI CGCG 2 cut(s) 102, 345
BspHI TCATGA 1 cut(s) 330
BspLI GGNNCC 7 cut(s) 217, 230, 627, 628, 723, 1414, 1634
BspOI GCTAGC 1 cut(s) 453
BspPI GGATC 2 cut(s) 437, 1268
BspTNI GGTCTC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 594, 696
BsrFI RCCGGY 1 cut(s) 852
BsrI ACTGG 5 cut(s) 34, 84, 526, 1215, 1261
BssAI RCCGGY 1 cut(s) 852
BssECI CCNNGG 4 cut(s) 779, 1283, 1491, 1704
BssMI GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
BssSI CACGAG 3 cut(s) 253, 480, 875
BssT1I CCWWGG 2 cut(s) 779, 1491
Bst2BI CACGAG 3 cut(s) 253, 480, 875
Bst2UI CCWGG 3 cut(s) 1284, 1637, 1706
Bst4CI ACNGT 4 cut(s) 369, 678, 916, 922
Bst6I CTCTTC 2 cut(s) 811, 1023
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 6 cut(s) 294, 451, 651, 854, 1313, 1317
BstDEI CTNAG 5 cut(s) 15, 375, 696, 798, 972
BstDSI CCRYGG 1 cut(s) 779
BstEII GGTNACC 1 cut(s) 1339
BstENI CCTNNNNNAGG 1 cut(s) 223
BstF5I GGATG 4 cut(s) 385, 742, 1279, 1687
BstFNI CGCG 2 cut(s) 102, 345
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 7 cut(s) 445, 874, 978, 1093, 1276, 1360, 1504
BstMAI GTCTC 2 cut(s) 28, 889
BstMBI GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
BstMWI GCNNNNNNNGC 3 cut(s) 706, 775, 1562
BstNI CCWGG 3 cut(s) 1284, 1637, 1706
BstNSI RCATGY 1 cut(s) 793
BstPI GGTNACC 1 cut(s) 1339
BstSCI CCNGG 4 cut(s) 120, 1282, 1635, 1704
BstSFI CTRYAG 1 cut(s) 981
BstUI CGCG 2 cut(s) 102, 345
BstV1I GCAGC 7 cut(s) 20, 673, 687, 808, 811, 1025, 1565
BstV2I GAAGAC 2 cut(s) 321, 332
BstX2I RGATCY 3 cut(s) 871, 975, 1090
BstYI RGATCY 3 cut(s) 871, 975, 1090
BsuRI GGCC 5 cut(s) 478, 593, 852, 1490, 1688
BtgI CCRYGG 1 cut(s) 779
BtgZI GCGATG 1 cut(s) 180
BtrI CACGTC 1 cut(s) 539
BtsCI GGATG 4 cut(s) 385, 742, 1279, 1687
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 3 cut(s) 27, 424, 1254
Cac8I GCNNGC 6 cut(s) 294, 451, 651, 854, 1313, 1317
CaiI CAGNNNCTG 2 cut(s) 206, 920
CciI TCATGA 1 cut(s) 330
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 852
Cfr13I GGNCC 5 cut(s) 228, 591, 626, 850, 1633
Csp6I GTAC 4 cut(s) 793, 1109, 1188, 1715
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 5 cut(s) 331, 780, 790, 1158, 1268
CviQI GTAC 4 cut(s) 793, 1109, 1188, 1715
DdeI CTNAG 5 cut(s) 15, 375, 696, 798, 972
DpnI GATC 7 cut(s) 444, 873, 977, 1092, 1275, 1359, 1503
DpnII GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
EaeI YGGCCR 1 cut(s) 1488
Eam1104I CTCTTC 2 cut(s) 811, 1023
EarI CTCTTC 2 cut(s) 811, 1023
Ecl136II GAGCTC 2 cut(s) 54, 802
Eco130I CCWWGG 2 cut(s) 779, 1491
Eco147I AGGCCT 1 cut(s) 1688
Eco24I GRGCYC 2 cut(s) 56, 804
Eco31I GGTCTC 1 cut(s) 889
Eco47I GGWCC 3 cut(s) 228, 626, 1633
Eco53kI GAGCTC 2 cut(s) 54, 802
Eco57I CTGAAG 1 cut(s) 1259
Eco72I CACGTG 1 cut(s) 497
Eco88I CYCGRG 1 cut(s) 223
Eco91I GGTNACC 1 cut(s) 1339
EcoICRI GAGCTC 2 cut(s) 54, 802
EcoNI CCTNNNNNAGG 1 cut(s) 223
EcoO109I RGGNCCY 3 cut(s) 228, 626, 1633
EcoO65I GGTNACC 1 cut(s) 1339
EcoRII CCWGG 3 cut(s) 1282, 1635, 1704
EcoT14I CCWWGG 2 cut(s) 779, 1491
EcoT38I GRGCYC 2 cut(s) 56, 804
ErhI CCWWGG 2 cut(s) 779, 1491
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 5 cut(s) 334, 783, 793, 1161, 1271
FaqI GGGAC 2 cut(s) 214, 612
FatI CATG 5 cut(s) 330, 779, 789, 1157, 1267
FauI CCCGC 3 cut(s) 240, 637, 691
FauNDI CATATG 1 cut(s) 1229
FbaI TGATCA 2 cut(s) 1357, 1501
Fnu4HI GCNGC 7 cut(s) 9, 687, 701, 822, 825, 1014, 1554
FokI GGATG 4 cut(s) 392, 749, 1266, 1694
FriOI GRGCYC 2 cut(s) 56, 804
Fsp4HI GCNGC 7 cut(s) 9, 687, 701, 822, 825, 1014, 1554
FspBI CTAG 3 cut(s) 450, 588, 1466
GlaI GCGC 1 cut(s) 12
GluI GCNGC 7 cut(s) 9, 687, 701, 822, 825, 1014, 1554
GsuI CTGGAG 2 cut(s) 67, 1287
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 5 cut(s) 478, 593, 852, 1490, 1688
HapII CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
HhaI GCGC 1 cut(s) 13
Hin1II CATG 5 cut(s) 334, 783, 793, 1161, 1271
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 1350
HindII GTYRAC 1 cut(s) 1350
HindIII AAGCTT 2 cut(s) 707, 1313
HinfI GANTC 6 cut(s) 33, 38, 203, 362, 1224, 1294
HpaI GTTAAC 1 cut(s) 1350
HpaII CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
HphI GGTGA 6 cut(s) 162, 275, 672, 1064, 1333, 1382
Hpy166II GTNNAC 1 cut(s) 1350
Hpy188I TCNGA 3 cut(s) 108, 799, 975
Hpy8I GTNNAC 1 cut(s) 1350
HpyAV CCTTC 6 cut(s) 53, 352, 735, 1160, 1508, 1634
HpyCH4III ACNGT 4 cut(s) 369, 678, 916, 922
HpyCH4IV ACGT 2 cut(s) 496, 538
HpyCH4V TGCA 6 cut(s) 545, 599, 689, 703, 827, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 706, 775, 1562
HpyF3I CTNAG 5 cut(s) 15, 375, 696, 798, 972
HpySE526I ACGT 2 cut(s) 496, 538
Hsp92II CATG 5 cut(s) 334, 783, 793, 1161, 1271
HspAI GCGC 1 cut(s) 11
KflI GGGWCCC 1 cut(s) 626
Kpn2I TCCGGA 2 cut(s) 733, 960
KroI GCCGGC 1 cut(s) 852
KroNI GCCGGC 1 cut(s) 854
Ksp22I TGATCA 2 cut(s) 1357, 1501
KspAI GTTAAC 1 cut(s) 1350
Kzo9I GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
LmnI GCTCC 6 cut(s) 51, 59, 221, 766, 1412, 1523
Lsp1109I GCAGC 7 cut(s) 20, 673, 687, 808, 811, 1025, 1565
LweI GCATC 2 cut(s) 462, 836
MaeI CTAG 3 cut(s) 450, 588, 1466
MaeII ACGT 2 cut(s) 496, 538
MaeIII GTNAC 6 cut(s) 490, 517, 613, 678, 916, 1339
MalI GATC 7 cut(s) 444, 873, 977, 1092, 1275, 1359, 1503
MboI GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
MboII GAAGA 6 cut(s) 317, 326, 332, 798, 1040, 1085
MfeI CAATTG 3 cut(s) 816, 1383, 1626
MflI RGATCY 3 cut(s) 871, 975, 1090
MhlI GDGCHC 3 cut(s) 56, 298, 804
MlsI TGGCCA 1 cut(s) 1490
MluNI TGGCCA 1 cut(s) 1490
MlyI GAGTC 2 cut(s) 197, 1233
MmeI TCCRAC 4 cut(s) 973, 1014, 1183, 1201
Mox20I TGGCCA 1 cut(s) 1490
MroI TCCGGA 2 cut(s) 733, 960
MroNI GCCGGC 1 cut(s) 852
MroXI GAANNNNTTC 1 cut(s) 42
MscI TGGCCA 1 cut(s) 1490
MseI TTAA 6 cut(s) 885, 1044, 1349, 1461, 1590, 1724
MslI CAYNNNNRTG 1 cut(s) 1726
Msp20I TGGCCA 1 cut(s) 1490
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
MspR9I CCNGG 4 cut(s) 122, 1284, 1637, 1706
MunI CAATTG 3 cut(s) 816, 1383, 1626
Mva1269I GAATGC 2 cut(s) 61, 660
MvaI CCWGG 3 cut(s) 1284, 1637, 1706
MvnI CGCG 2 cut(s) 102, 345
MwoI GCNNNNNNNGC 3 cut(s) 706, 775, 1562
NaeI GCCGGC 1 cut(s) 854
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 779
NdeI CATATG 1 cut(s) 1229
NdeII GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
NgoMIV GCCGGC 1 cut(s) 852
NheI GCTAGC 1 cut(s) 449
NlaIII CATG 5 cut(s) 334, 783, 793, 1161, 1271
NlaIV GGNNCC 7 cut(s) 217, 230, 627, 628, 723, 1414, 1634
NmuCI GTSAC 4 cut(s) 490, 613, 678, 1339
NspI RCATGY 1 cut(s) 793
PaeR7I CTCGAG 1 cut(s) 223
PagI TCATGA 1 cut(s) 330
PceI AGGCCT 1 cut(s) 1688
PciI ACATGT 1 cut(s) 789
PctI GAATGC 2 cut(s) 61, 660
PdiI GCCGGC 1 cut(s) 854
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 4 cut(s) 33, 38, 362, 1294
PflFI GACNNNGTC 2 cut(s) 206, 1430
PflMI CCANNNNNTGG 1 cut(s) 1549
PfoI TCCNGGA 1 cut(s) 1635
PkrI GCNGC 7 cut(s) 10, 688, 702, 823, 826, 1015, 1555
PleI GAGTC 2 cut(s) 197, 1232
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 2 cut(s) 197, 1232
Ppu21I YACGTR 1 cut(s) 497
PpuMI RGGWCCY 3 cut(s) 228, 626, 1633
PscI ACATGT 1 cut(s) 789
PshBI ATTAAT 1 cut(s) 1724
PsiI TTATAA 2 cut(s) 557, 1616
Psp124BI GAGCTC 2 cut(s) 56, 804
Psp5II RGGWCCY 3 cut(s) 228, 626, 1633
Psp6I CCWGG 3 cut(s) 1282, 1635, 1704
PspCI CACGTG 1 cut(s) 497
PspEI GGTNACC 1 cut(s) 1339
PspGI CCWGG 3 cut(s) 1282, 1635, 1704
PspN4I GGNNCC 7 cut(s) 217, 230, 627, 628, 723, 1414, 1634
PspPI GGNCC 5 cut(s) 228, 591, 626, 850, 1633
PspPPI RGGWCCY 3 cut(s) 228, 626, 1633
PspXI VCTCGAGB 1 cut(s) 223
PstNI CAGNNNCTG 2 cut(s) 206, 920
PsuI RGATCY 3 cut(s) 871, 975, 1090
PsyI GACNNNGTC 2 cut(s) 206, 1430
RsaI GTAC 4 cut(s) 794, 1110, 1189, 1716
RsaNI GTAC 4 cut(s) 793, 1109, 1188, 1715
RseI CAYNNNNRTG 1 cut(s) 1726
SacI GAGCTC 2 cut(s) 56, 804
SaqAI TTAA 6 cut(s) 885, 1044, 1349, 1461, 1590, 1724
SatI GCNGC 7 cut(s) 9, 687, 701, 822, 825, 1014, 1554
Sau3AI GATC 7 cut(s) 442, 871, 975, 1090, 1273, 1357, 1501
Sau96I GGNCC 5 cut(s) 228, 591, 626, 850, 1633
ScaI AGTACT 1 cut(s) 1189
SchI GAGTC 2 cut(s) 197, 1233
ScrFI CCNGG 4 cut(s) 122, 1284, 1637, 1706
SduI GDGCHC 3 cut(s) 56, 298, 804
SfaNI GCATC 2 cut(s) 462, 836
SfcI CTRYAG 1 cut(s) 981
Sfr274I CTCGAG 1 cut(s) 223
SinI GGWCC 3 cut(s) 228, 626, 1633
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 1726
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
SseBI AGGCCT 1 cut(s) 1688
SsiI CCGC 6 cut(s) 131, 233, 345, 630, 684, 1598
SspMI CTAG 3 cut(s) 450, 588, 1466
SstI GAGCTC 2 cut(s) 56, 804
StuI AGGCCT 1 cut(s) 1688
StyD4I CCNGG 4 cut(s) 120, 1282, 1635, 1704
StyI CCWWGG 2 cut(s) 779, 1491
TaaI ACNGT 4 cut(s) 369, 678, 916, 922
TaiI ACGT 2 cut(s) 499, 541
TaqI TCGA 4 cut(s) 36, 94, 224, 664
TatI WGTACW 3 cut(s) 792, 1187, 1714
TfiI GAWTC 4 cut(s) 33, 38, 362, 1294
Tru1I TTAA 6 cut(s) 885, 1044, 1349, 1461, 1590, 1724
Tru9I TTAA 6 cut(s) 885, 1044, 1349, 1461, 1590, 1724
TscAI CASTG 3 cut(s) 34, 424, 1261
TseFI GTSAC 4 cut(s) 490, 613, 678, 1339
TseI GCWGC 7 cut(s) 8, 686, 700, 821, 824, 1013, 1553
Tsp45I GTSAC 4 cut(s) 490, 613, 678, 1339
TspDTI ATGAA 8 cut(s) 54, 347, 426, 594, 717, 1020, 1256, 1388
TspGWI ACGGA 1 cut(s) 955
TspRI CASTG 3 cut(s) 34, 424, 1261
Tth111I GACNNNGTC 2 cut(s) 206, 1430
Van91I CCANNNNNTGG 1 cut(s) 1549
VpaK11BI GGWCC 3 cut(s) 228, 626, 1633
VspI ATTAAT 1 cut(s) 1724
XagI CCTNNNNNAGG 1 cut(s) 223
XapI RAATTY 3 cut(s) 352, 422, 1288
XbaI TCTAGA 1 cut(s) 1465
XceI RCATGY 1 cut(s) 793
XhoI CTCGAG 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 3 cut(s) 450, 588, 1466
ZrmI AGTACT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.