RchiOBHm_Chr4g0385111

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011327
Physical Location & Seq
Forward (+)
592201 .. 600477
8277 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35862

Sequence Viewer

Length: 2385 bp
ATGGAGGCAGCGCCTCAGCTCGTCTCCACTGGAATCGAATCCCTTCGCCGGAGCTCCAAGCATTCATTCCCGACCACAACCTCTAGTAGAGTTCGAGTTCGCGTTCCGAACCGATTGAACCGGGTTCTTGCGGTTGCCACCGACCCCAAACCCACCAAGTCCGGCTCATCACCATCAAAGCCACCCAAATCTCCATCGCCAGACTCTGTCAATGGCTCCTCCTCGAGGCCTCCGCCTATCAATGGAGTTTCCACGAGAATTGGAGATGTTTCAAAGGAAATCAAAAGGGTGCGAGCACAGATGGAAGAAAACGAAGACTTGGCAATACTCATGAAAGGTCTTCGCGGACAAAATTTGAAGGATTCACAGTTTGCTGAGGATGATGTTCAGCTTCGTCTTGTTGAGGTAGATGAAAGCAGTGAATTTTTGCCTGTGGTATATGATCCTGCTAGCATCTCTGCTTATTGGGGAAAACGGCCTCGTGCTGTTGTGACACGTGTAATTCAATTACTCTCTGTAACTGGGGGTTTCCTTTCACGTCTTGCAGGGGATTTTATAAACAAGAAAATCAAAGAGAATGAAGTTGCTAGGGCCATTGAAATAAGGGAAATTGTGACCTCTTTGGGTCCCGCATATATCAAACTTGGGCAAGCATTGAGCATTCGACCAGATATACTATCACCCGCTGCAATGACTGAGCTGCAAAAGCTTTGTGATAAGGTTCCTTCATTTCCGGATGATATAGCTATGGCTCTCATTGAGGAGGAGCTTGGTCAGCCATGGCAAAACATCTACTCTGAGCTCTCTTCCTCTCCAATTGCTGCTGCATCTCTTGGGCAAGTATATAAGGGCCGGCTAAAAGAAAATGGAGATCTTGTGGCTGTTAAAGTGCAGAGACCTTTTGTTCTTGAAACAGTAACTGTTGATTTGTTTATCATACGGAACTTGGGTTTGGTTCTCCGGAGGTTTCCACAGATCTCTGTAGATGTGGTTGGATTAGTTGATGAATGGGCAGCTCGTTTTTTTGAAGAGTTGGATTATATTAATGAGGGTGAGAATGGACTACTCTTTGCTGAAATGATGAGAAAAGATCTTCCACAGGTGGTTGTACCAAAAACCTACCCAAAATATACATCAAGAAAGGTTCTTACTACATCATGGTTAGAAGGAGAGAAGCTATCGCAAAGTACTGAAAGTGATGTTGGAGAACTGGTCAATGTTGGAGTCATATGCTACCTGAAGCAGTTGCTTGACACTGGGTTATTTCATGCTGATCCTCATCCTGGGAATTTGATTCGCACTCCAGATGGCAAGCTTGCCATACTTGATTTTGGTCTGGTCACCAAGTTAACTGATGATCAAAAGTATGGAATGATCGAGGCAATTGCTCACCTTATTCATCGTGATTATGGAGCCATAGTCAAAGACTTTGTCAAGCTTGGTTTTATTGACGAGGGGGTAAATCTAGAACCTATTTTGCCTGTTCTGGCCAAGGTTTTTGATCAGGCTCTTGAAGGTGGAGGAGCAAAAAATATCAACTTCCAAGACTTGGCAGCAGATTTGGCACAAATCACATTTGACTATCCATTTAAGATACCGCCTTATTTCGCTCTTATAATTAGAGCAATTGGGGTCCTGGAAGGCATAGCTTTAGTGGGAAATCCTGATTTTGCTATTGTGGATGAGGCCTATCCATATATTGCCCAGAGGCTACTGACAGATGAATCCCCACGGTTAAGGAATGCTTTACGTTACACAATTTATGGGAAATCTGGAGTTTTTGATGCGGAAAGATTTATTGATGTCATGCAAGCCTTTGAGAGTTTCATAACTGCTGCTAAAAGTGGAGGTGGAGAGGAAATGAATGGCGATATGGCTGAACTTGGACTTTTGCAAAGTCGAACAGAGTATACCCTTCCTGGATTTGCATCAAATGTGCAACCAGTTCAAACAAGGGCGGCTTTAGCATTTGTACTGTCTGATAAAGGAAACTTTTTCAGAGAATTTCTTCTGGATGAGATTGTGAAAGGCATTGATGCAGTTACAAGAGAACAATTGGTTCGTGCAATGGCACTTCTTGGATTCGGGAATGCTCTTCCAGTTTTCAGCATGGCTCCTAGTTTTGGGCCTTTCAAGCCTGCAGGACTTCTACCAACAATAACTGAGGAGGATAGAGTTATATTGAACAATGTTCAAAGAGTAGTCGAGTTCCTGGCTGCAGGGAGTTCGATATCAAGAACATCCAATCAGGAATTAAATGTGTCTCAGGTTATCCAAGAGTTTCTTCCTGTTATGCCGAGTATCTCTTCCAAAGTGCTCCCTGAAGTGCTCAGCCGATTATCTTCACGGGTAATAGCACGTGTGATTAGAGATACAATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 APH

Protein Analysis

794

Amino Acids

87.36

Weight (kDa)

5.41

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 237 - 475 5.3e-63 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 557, 1616
AbsI CCTCGAGG 1 cut(s) 223
AccB7I CCANNNNNTGG 1 cut(s) 1549
AccI GTMKAC 1 cut(s) 1910
AccII CGCG 2 cut(s) 102, 345
AccIII TCCGGA 2 cut(s) 733, 960
AciI CCGC 8 cut(s) 131, 233, 345, 630, 684, 1598, 1787, 1958
AclWI GGATC 2 cut(s) 437, 1268
AcoI YGGCCR 1 cut(s) 1488
AcsI RAATTY 4 cut(s) 352, 422, 1288, 2003
AcuI CTGAAG 2 cut(s) 1259, 2343
AcvI CACGTG 2 cut(s) 497, 2360
AfaI GTAC 3 cut(s) 1110, 1189, 1974
AfiI CCNNNNNNNGG 6 cut(s) 48, 225, 242, 1283, 1549, 2123
AflIII ACRYGT 3 cut(s) 494, 496, 2359
AjiI CACGTC 1 cut(s) 539
AjnI CCWGG 4 cut(s) 1282, 1635, 1918, 2211
AjuI GAANNNNNNNTTGG 8 cut(s) 50, 82, 935, 967, 1185, 1217, 2268, 2300
Alw21I GWGCWC 5 cut(s) 56, 298, 804, 2319, 2331
Alw26I GTCTC 3 cut(s) 28, 889, 2268
AlwI GGATC 2 cut(s) 437, 1268
AlwNI CAGNNNCTG 2 cut(s) 206, 920
Ama87I CYCGRG 1 cut(s) 223
Aor13HI TCCGGA 2 cut(s) 733, 960
AoxI GGCC 7 cut(s) 227, 476, 591, 850, 1488, 1686, 2126
ApeKI GCWGC 9 cut(s) 8, 686, 700, 821, 824, 1013, 1553, 1835, 2216
ApoI RAATTY 4 cut(s) 352, 422, 1288, 2003
ArsI GACNNNNNNTTYG 2 cut(s) 1416, 1448
AseI ATTAAT 1 cut(s) 1044
Asp700I GAANNNNTTC 2 cut(s) 42, 2007
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 5 cut(s) 591, 626, 850, 1633, 2126
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 5 cut(s) 162, 672, 1064, 1333, 1382
AsuNHI GCTAGC 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 2 cut(s) 626, 1633
BalI TGGCCA 1 cut(s) 1490
BanII GRGCYC 2 cut(s) 56, 804
BauI CACGAG 2 cut(s) 253, 480
BbrPI CACGTG 2 cut(s) 497, 2360
BbsI GAAGAC 2 cut(s) 321, 332
Bbv12I GWGCWC 5 cut(s) 56, 298, 804, 2319, 2331
BbvCI CCTCAGC 2 cut(s) 15, 375
BbvI GCAGC 9 cut(s) 20, 673, 687, 808, 811, 1025, 1565, 1822, 2203
BccI CCATC 4 cut(s) 181, 202, 295, 1301
BceAI ACGGC 1 cut(s) 491
BcgI CGANNNNNNTGC 4 cut(s) 1367, 1401, 2208, 2242
BciT130I CCWGG 4 cut(s) 1284, 1637, 1920, 2213
BclI TGATCA 2 cut(s) 1357, 1501
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 3 cut(s) 28, 889, 2268
BfaI CTAG 5 cut(s) 84, 450, 588, 1466, 2118
BfmI CTRYAG 3 cut(s) 981, 2139, 2217
BfoI RGCGCY 1 cut(s) 14
BglII AGATCT 3 cut(s) 871, 975, 1090
BlpI GCTNAGC 1 cut(s) 2330
BmcAI AGTACT 1 cut(s) 1189
Bme1390I CCNGG 5 cut(s) 122, 1284, 1637, 1920, 2213
Bme18I GGWCC 2 cut(s) 626, 1633
BmeT110I CYCGRG 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 539
BmgT120I GGNCC 5 cut(s) 591, 626, 850, 1633, 2126
BmiI GGNNCC 7 cut(s) 217, 627, 628, 723, 1414, 1634, 2115
BmrFI CCNGG 5 cut(s) 122, 1284, 1637, 1920, 2213
BmrI ACTGGG 2 cut(s) 531, 1266
BmsI GCATC 5 cut(s) 462, 836, 1774, 1937, 2026
BmtI GCTAGC 1 cut(s) 453
BmuI ACTGGG 2 cut(s) 531, 1266
BpiI GAAGAC 2 cut(s) 321, 332
BpmI CTGGAG 2 cut(s) 1287, 1794
Bpu10I CCTNAGC 2 cut(s) 15, 375
Bpu1102I GCTNAGC 1 cut(s) 2330
BpuMI CCSGG 1 cut(s) 122
BsaAI YACGTR 2 cut(s) 497, 2360
BsaBI GATNNNNATC 2 cut(s) 1278, 1927
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 4 cut(s) 779, 1283, 1491, 1730
BsaWI WCCGGW 2 cut(s) 733, 960
BsaXI ACNNNNNCTCC 6 cut(s) 861, 891, 1197, 1227, 1404, 1434
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 225, 242, 1283, 1549, 2123
Bse118I RCCGGY 1 cut(s) 852
Bse1I ACTGG 6 cut(s) 34, 526, 1215, 1261, 1943, 2099
Bse3DI GCAATG 2 cut(s) 696, 2073
Bse8I GATNNNNATC 2 cut(s) 1278, 1927
BseAI TCCGGA 2 cut(s) 733, 960
BseBI CCWGG 4 cut(s) 1284, 1637, 1920, 2213
BseDI CCNNGG 4 cut(s) 779, 1283, 1491, 1730
BseGI GGATG 6 cut(s) 385, 742, 1279, 1687, 2020, 2240
BseJI GATNNNNATC 2 cut(s) 1278, 1927
BseLI CCNNNNNNNGG 6 cut(s) 48, 225, 242, 1283, 1549, 2123
BseMI GCAATG 2 cut(s) 696, 2073
BseMII CTCAG 7 cut(s) 29, 366, 687, 789, 2154, 2279, 2344
BseNI ACTGG 6 cut(s) 34, 526, 1215, 1261, 1943, 2099
BseRI GAGGAG 6 cut(s) 208, 211, 776, 779, 1536, 2180
BseXI GCAGC 9 cut(s) 20, 673, 687, 808, 811, 1025, 1565, 1822, 2203
BsgI GTGCAG 1 cut(s) 911
Bsh1236I CGCG 2 cut(s) 102, 345
BshFI GGCC 7 cut(s) 229, 478, 593, 852, 1490, 1688, 2128
BsiHKAI GWGCWC 5 cut(s) 56, 298, 804, 2319, 2331
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
BslFI GGGAC 1 cut(s) 612
BslI CCNNNNNNNGG 6 cut(s) 48, 225, 242, 1283, 1549, 2123
BsmAI GTCTC 3 cut(s) 28, 889, 2268
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 612
BsmI GAATGC 4 cut(s) 61, 660, 1747, 2095
BsnI GGCC 7 cut(s) 229, 478, 593, 852, 1490, 1688, 2128
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 5 cut(s) 56, 298, 804, 2319, 2331
Bsp13I TCCGGA 2 cut(s) 733, 960
Bsp143I GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
Bsp1720I GCTNAGC 1 cut(s) 2330
Bsp19I CCATGG 1 cut(s) 779
BspACI CCGC 8 cut(s) 131, 233, 345, 630, 684, 1598, 1787, 1958
BspANI GGCC 7 cut(s) 229, 478, 593, 852, 1490, 1688, 2128
BspCNI CTCAG 7 cut(s) 28, 367, 688, 790, 2155, 2278, 2343
BspEI TCCGGA 2 cut(s) 733, 960
BspFNI CGCG 2 cut(s) 102, 345
BspHI TCATGA 1 cut(s) 330
BspLI GGNNCC 7 cut(s) 217, 627, 628, 723, 1414, 1634, 2115
BspMAI CTGCAG 2 cut(s) 2143, 2221
BspOI GCTAGC 1 cut(s) 453
BspPI GGATC 2 cut(s) 437, 1268
BspQI GCTCTTC 1 cut(s) 2100
BspTNI GGTCTC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 696, 2073
BsrFI RCCGGY 1 cut(s) 852
BsrI ACTGG 6 cut(s) 34, 526, 1215, 1261, 1943, 2099
BssAI RCCGGY 1 cut(s) 852
BssECI CCNNGG 4 cut(s) 779, 1283, 1491, 1730
BssMI GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
BssNAI GTATAC 1 cut(s) 1911
BssSI CACGAG 2 cut(s) 253, 480
BssT1I CCWWGG 2 cut(s) 779, 1491
Bst1107I GTATAC 1 cut(s) 1911
Bst2BI CACGAG 2 cut(s) 253, 480
Bst2UI CCWGG 4 cut(s) 1284, 1637, 1920, 2213
Bst4CI ACNGT 5 cut(s) 369, 916, 922, 1734, 1977
Bst6I CTCTTC 4 cut(s) 811, 1023, 2100, 2311
BstBAI YACGTR 2 cut(s) 497, 2360
BstC8I GCNNGC 8 cut(s) 294, 451, 651, 854, 1313, 1317, 1812, 2139
BstDEI CTNAG 7 cut(s) 15, 375, 696, 798, 2163, 2265, 2330
BstDSI CCRYGG 2 cut(s) 779, 1730
BstEII GGTNACC 1 cut(s) 1339
BstENI CCTNNNNNAGG 1 cut(s) 223
BstF5I GGATG 6 cut(s) 385, 742, 1279, 1687, 2020, 2240
BstFNI CGCG 2 cut(s) 102, 345
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 8 cut(s) 445, 874, 978, 1093, 1276, 1360, 1377, 1504
BstMAI GTCTC 3 cut(s) 28, 889, 2268
BstMBI GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
BstMWI GCNNNNNNNGC 5 cut(s) 706, 775, 1562, 1964, 2134
BstNI CCWGG 4 cut(s) 1284, 1637, 1920, 2213
BstPI GGTNACC 1 cut(s) 1339
BstSCI CCNGG 5 cut(s) 120, 1282, 1635, 1918, 2211
BstSFI CTRYAG 3 cut(s) 981, 2139, 2217
BstUI CGCG 2 cut(s) 102, 345
BstV1I GCAGC 9 cut(s) 20, 673, 687, 808, 811, 1025, 1565, 1822, 2203
BstV2I GAAGAC 2 cut(s) 321, 332
BstX2I RGATCY 3 cut(s) 871, 975, 1090
BstYI RGATCY 3 cut(s) 871, 975, 1090
BstZ17I GTATAC 1 cut(s) 1911
BsuRI GGCC 7 cut(s) 229, 478, 593, 852, 1490, 1688, 2128
BtgI CCRYGG 2 cut(s) 779, 1730
BtgZI GCGATG 1 cut(s) 180
BtrI CACGTC 1 cut(s) 539
BtsCI GGATG 6 cut(s) 385, 742, 1279, 1687, 2020, 2240
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 3 cut(s) 27, 424, 1254
Cac8I GCNNGC 8 cut(s) 294, 451, 651, 854, 1313, 1317, 1812, 2139
CaiI CAGNNNCTG 2 cut(s) 206, 920
CciI TCATGA 1 cut(s) 330
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 852
Cfr13I GGNCC 5 cut(s) 591, 626, 850, 1633, 2126
Csp6I GTAC 3 cut(s) 1109, 1188, 1973
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 6 cut(s) 331, 780, 1158, 1268, 1807, 2110
CviQI GTAC 3 cut(s) 1109, 1188, 1973
DdeI CTNAG 7 cut(s) 15, 375, 696, 798, 2163, 2265, 2330
DpnI GATC 8 cut(s) 444, 873, 977, 1092, 1275, 1359, 1376, 1503
DpnII GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
EaeI YGGCCR 1 cut(s) 1488
Eam1104I CTCTTC 4 cut(s) 811, 1023, 2100, 2311
EarI CTCTTC 4 cut(s) 811, 1023, 2100, 2311
EciI GGCGGA 1 cut(s) 222
Ecl136II GAGCTC 2 cut(s) 54, 802
Eco130I CCWWGG 2 cut(s) 779, 1491
Eco147I AGGCCT 2 cut(s) 229, 1688
Eco24I GRGCYC 2 cut(s) 56, 804
Eco31I GGTCTC 1 cut(s) 889
Eco32I GATATC 1 cut(s) 2232
Eco47I GGWCC 2 cut(s) 626, 1633
Eco53kI GAGCTC 2 cut(s) 54, 802
Eco57I CTGAAG 2 cut(s) 1259, 2343
Eco72I CACGTG 2 cut(s) 497, 2360
Eco88I CYCGRG 1 cut(s) 223
Eco91I GGTNACC 1 cut(s) 1339
EcoICRI GAGCTC 2 cut(s) 54, 802
EcoNI CCTNNNNNAGG 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 626, 1633
EcoO65I GGTNACC 1 cut(s) 1339
EcoRII CCWGG 4 cut(s) 1282, 1635, 1918, 2211
EcoRV GATATC 1 cut(s) 2232
EcoT14I CCWWGG 2 cut(s) 779, 1491
EcoT38I GRGCYC 2 cut(s) 56, 804
ErhI CCWWGG 2 cut(s) 779, 1491
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 6 cut(s) 334, 783, 1161, 1271, 1810, 2113
FalI AAGNNNNNCTT 6 cut(s) 1729, 1761, 1945, 1977, 2268, 2300
FaqI GGGAC 1 cut(s) 612
FatI CATG 6 cut(s) 330, 779, 1157, 1267, 1806, 2109
FauI CCCGC 2 cut(s) 637, 691
FauNDI CATATG 1 cut(s) 1229
FbaI TGATCA 2 cut(s) 1357, 1501
FblI GTMKAC 1 cut(s) 1910
FokI GGATG 6 cut(s) 392, 749, 1266, 1694, 2027, 2227
FriOI GRGCYC 2 cut(s) 56, 804
FspBI CTAG 5 cut(s) 84, 450, 588, 1466, 2118
GlaI GCGC 1 cut(s) 12
GsuI CTGGAG 2 cut(s) 1287, 1794
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 7 cut(s) 229, 478, 593, 852, 1490, 1688, 2128
HapII CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
HhaI GCGC 1 cut(s) 13
Hin1II CATG 6 cut(s) 334, 783, 1161, 1271, 1810, 2113
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 1350
HindII GTYRAC 1 cut(s) 1350
HindIII AAGCTT 3 cut(s) 707, 1313, 1436
HinfI GANTC 8 cut(s) 33, 38, 203, 362, 1224, 1294, 1724, 2082
HpaI GTTAAC 1 cut(s) 1350
HpaII CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
HphI GGTGA 5 cut(s) 162, 672, 1064, 1333, 1382
Hpy166II GTNNAC 2 cut(s) 1350, 1911
Hpy188I TCNGA 4 cut(s) 108, 799, 1981, 2000
Hpy8I GTNNAC 2 cut(s) 1350, 1911
HpyAV CCTTC 7 cut(s) 53, 352, 735, 1160, 1508, 1634, 1925
HpyCH4III ACNGT 5 cut(s) 369, 916, 922, 1734, 1977
HpyCH4IV ACGT 4 cut(s) 496, 538, 1750, 2359
HpyF10VI GCNNNNNNNGC 5 cut(s) 706, 775, 1562, 1964, 2134
HpyF3I CTNAG 7 cut(s) 15, 375, 696, 798, 2163, 2265, 2330
HpySE526I ACGT 4 cut(s) 496, 538, 1750, 2359
Hsp92II CATG 6 cut(s) 334, 783, 1161, 1271, 1810, 2113
HspAI GCGC 1 cut(s) 11
KflI GGGWCCC 1 cut(s) 626
Kpn2I TCCGGA 2 cut(s) 733, 960
KroI GCCGGC 1 cut(s) 852
KroNI GCCGGC 1 cut(s) 854
Ksp22I TGATCA 2 cut(s) 1357, 1501
KspAI GTTAAC 1 cut(s) 1350
Kzo9I GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
LguI GCTCTTC 1 cut(s) 2100
LmnI GCTCC 8 cut(s) 51, 59, 221, 766, 1412, 1523, 2119, 2322
Lsp1109I GCAGC 9 cut(s) 20, 673, 687, 808, 811, 1025, 1565, 1822, 2203
LweI GCATC 5 cut(s) 462, 836, 1774, 1937, 2026
MaeI CTAG 5 cut(s) 84, 450, 588, 1466, 2118
MaeII ACGT 4 cut(s) 496, 538, 1750, 2359
MaeIII GTNAC 7 cut(s) 490, 517, 613, 916, 1339, 1751, 2041
MalI GATC 8 cut(s) 444, 873, 977, 1092, 1275, 1359, 1376, 1503
MboI GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
MfeI CAATTG 4 cut(s) 816, 1383, 1626, 2054
MflI RGATCY 3 cut(s) 871, 975, 1090
MhlI GDGCHC 5 cut(s) 56, 298, 804, 2319, 2331
MlsI TGGCCA 1 cut(s) 1490
MluNI TGGCCA 1 cut(s) 1490
MlyI GAGTC 2 cut(s) 197, 1233
MmeI TCCRAC 4 cut(s) 973, 1014, 1183, 1201
Mox20I TGGCCA 1 cut(s) 1490
MroI TCCGGA 2 cut(s) 733, 960
MroNI GCCGGC 1 cut(s) 852
MroXI GAANNNNTTC 2 cut(s) 42, 2007
MscI TGGCCA 1 cut(s) 1490
MseI TTAA 6 cut(s) 885, 1044, 1349, 1590, 1736, 2255
Msp20I TGGCCA 1 cut(s) 1490
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 6 cut(s) 49, 121, 162, 734, 853, 961
MspR9I CCNGG 5 cut(s) 122, 1284, 1637, 1920, 2213
MunI CAATTG 4 cut(s) 816, 1383, 1626, 2054
Mva1269I GAATGC 4 cut(s) 61, 660, 1747, 2095
MvaI CCWGG 4 cut(s) 1284, 1637, 1920, 2213
MvnI CGCG 2 cut(s) 102, 345
MwoI GCNNNNNNNGC 5 cut(s) 706, 775, 1562, 1964, 2134
NaeI GCCGGC 1 cut(s) 854
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 779
NdeI CATATG 1 cut(s) 1229
NdeII GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
NgoMIV GCCGGC 1 cut(s) 852
NheI GCTAGC 1 cut(s) 449
NlaIII CATG 6 cut(s) 334, 783, 1161, 1271, 1810, 2113
NlaIV GGNNCC 7 cut(s) 217, 627, 628, 723, 1414, 1634, 2115
NmeAIII GCCGAG 1 cut(s) 2322
NmuCI GTSAC 3 cut(s) 490, 613, 1339
PaeR7I CTCGAG 1 cut(s) 223
PagI TCATGA 1 cut(s) 330
PceI AGGCCT 2 cut(s) 229, 1688
PciSI GCTCTTC 1 cut(s) 2100
PctI GAATGC 4 cut(s) 61, 660, 1747, 2095
PdiI GCCGGC 1 cut(s) 854
PdmI GAANNNNTTC 2 cut(s) 42, 2007
PfeI GAWTC 6 cut(s) 33, 38, 362, 1294, 1724, 2082
PflFI GACNNNGTC 2 cut(s) 206, 1430
PflMI CCANNNNNTGG 1 cut(s) 1549
PfoI TCCNGGA 2 cut(s) 1635, 1918
PleI GAGTC 2 cut(s) 197, 1232
PmaCI CACGTG 2 cut(s) 497, 2360
PmlI CACGTG 2 cut(s) 497, 2360
PpsI GAGTC 2 cut(s) 197, 1232
Ppu21I YACGTR 2 cut(s) 497, 2360
PpuMI RGGWCCY 2 cut(s) 626, 1633
PshBI ATTAAT 1 cut(s) 1044
PsiI TTATAA 2 cut(s) 557, 1616
Psp124BI GAGCTC 2 cut(s) 56, 804
Psp5II RGGWCCY 2 cut(s) 626, 1633
Psp6I CCWGG 4 cut(s) 1282, 1635, 1918, 2211
PspCI CACGTG 2 cut(s) 497, 2360
PspEI GGTNACC 1 cut(s) 1339
PspGI CCWGG 4 cut(s) 1282, 1635, 1918, 2211
PspN4I GGNNCC 7 cut(s) 217, 627, 628, 723, 1414, 1634, 2115
PspPI GGNCC 5 cut(s) 591, 626, 850, 1633, 2126
PspPPI RGGWCCY 2 cut(s) 626, 1633
PspXI VCTCGAGB 1 cut(s) 223
PsrI GAACNNNNNNTAC 2 cut(s) 1894, 1926
PstI CTGCAG 2 cut(s) 2143, 2221
PstNI CAGNNNCTG 2 cut(s) 206, 920
PsuI RGATCY 3 cut(s) 871, 975, 1090
PsyI GACNNNGTC 2 cut(s) 206, 1430
RsaI GTAC 3 cut(s) 1110, 1189, 1974
RsaNI GTAC 3 cut(s) 1109, 1188, 1973
SacI GAGCTC 2 cut(s) 56, 804
SapI GCTCTTC 1 cut(s) 2100
SaqAI TTAA 6 cut(s) 885, 1044, 1349, 1590, 1736, 2255
Sau3AI GATC 8 cut(s) 442, 871, 975, 1090, 1273, 1357, 1374, 1501
Sau96I GGNCC 5 cut(s) 591, 626, 850, 1633, 2126
SbfI CCTGCAGG 1 cut(s) 2143
ScaI AGTACT 1 cut(s) 1189
SchI GAGTC 2 cut(s) 197, 1233
ScrFI CCNGG 5 cut(s) 122, 1284, 1637, 1920, 2213
SdaI CCTGCAGG 1 cut(s) 2143
SduI GDGCHC 5 cut(s) 56, 298, 804, 2319, 2331
SfaNI GCATC 5 cut(s) 462, 836, 1774, 1937, 2026
SfcI CTRYAG 3 cut(s) 981, 2139, 2217
Sfr274I CTCGAG 1 cut(s) 223
SinI GGWCC 2 cut(s) 626, 1633
SlaI CTCGAG 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse8387I CCTGCAGG 1 cut(s) 2143
SseBI AGGCCT 2 cut(s) 229, 1688
SsiI CCGC 8 cut(s) 131, 233, 345, 630, 684, 1598, 1787, 1958
SspMI CTAG 5 cut(s) 84, 450, 588, 1466, 2118
SstI GAGCTC 2 cut(s) 56, 804
StuI AGGCCT 2 cut(s) 229, 1688
StyD4I CCNGG 5 cut(s) 120, 1282, 1635, 1918, 2211
StyI CCWWGG 2 cut(s) 779, 1491
TaaI ACNGT 5 cut(s) 369, 916, 922, 1734, 1977
TaiI ACGT 4 cut(s) 499, 541, 1753, 2362
TaqI TCGA 8 cut(s) 36, 94, 224, 664, 1377, 1900, 2205, 2228
TatI WGTACW 2 cut(s) 1187, 1972
TauI GCSGC 1 cut(s) 1961
TfiI GAWTC 6 cut(s) 33, 38, 362, 1294, 1724, 2082
Tru1I TTAA 6 cut(s) 885, 1044, 1349, 1590, 1736, 2255
Tru9I TTAA 6 cut(s) 885, 1044, 1349, 1590, 1736, 2255
TscAI CASTG 3 cut(s) 34, 424, 1261
TseFI GTSAC 3 cut(s) 490, 613, 1339
TseI GCWGC 9 cut(s) 8, 686, 700, 821, 824, 1013, 1553, 1835, 2216
Tsp45I GTSAC 3 cut(s) 490, 613, 1339
TspGWI ACGGA 1 cut(s) 955
TspRI CASTG 3 cut(s) 34, 424, 1261
Tth111I GACNNNGTC 2 cut(s) 206, 1430
Van91I CCANNNNNTGG 1 cut(s) 1549
VpaK11BI GGWCC 2 cut(s) 626, 1633
VspI ATTAAT 1 cut(s) 1044
XagI CCTNNNNNAGG 1 cut(s) 223
XapI RAATTY 4 cut(s) 352, 422, 1288, 2003
XbaI TCTAGA 1 cut(s) 1465
XhoI CTCGAG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 1910
XmnI GAANNNNTTC 2 cut(s) 42, 2007
XspI CTAG 5 cut(s) 84, 450, 588, 1466, 2118
ZrmI AGTACT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.